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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prudnikova S. V., Vinogradova O. N., Trusova M. Y.
Заглавие : Specific character of bacterial biodegradation of polyhydroxyalkanoates with different chemical structure in soil
Место публикации : Doklad. Biochem. Biophys.: Maik Nauka Publishing / Springer SBM, 2017. - Vol. 473, Is. 1. - С. 94-97. - ISSN 16076729 (ISSN) , DOI 10.1134/S1607672917010185
Аннотация: The study addresses the influence of the physicochemical properties of the reserve cellular macromolecules (polyhydroxyalkanoates, PHA) with different chemical composition on their biodegradation in the agro-transformed field soil of the Siberian region (Krasnoyarsk Territory, Russia). It was shown that the degradation of the PHA samples depends on the degree of polymer crystallinity (Cx). For the first time, it was shown that the range of PHA-degrading microorganisms differs for each of PHA types. The study defines the primary degraders specific to each PHA type and common to all types of examined polymers. © 2017, Pleiades Publishing, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Larionova, Marina D., Markova, Svetlana V., Vysotski, Eugene S.
Заглавие : The novel extremely psychrophilic luciferase from Metridia longa: Properties of a high-purity protein produced in insect cells
Колич.характеристики :7 с
Коллективы : Bayer AG (Germany); Russian Academy of Sciences [01201351504]; RFBR; Government of Krasnoyarsk Territory [16-44-242099]
Место публикации : Biochem. Biophys. Res. Commun.: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2017. - Vol. 483, Is. 1. - С. 772-778. - ISSN 0006-291X, DOI 10.1016/j.bbrc.2016.12.067. - ISSN 1090-2104(eISSN)
Примечания : Cited References:24. - The cloning of cDNAs encoding MLuc2 isoforms of M. longa was supported by Bayer AG (Germany) and the state budget allocated to the fundamental research at the Russian Academy of Sciences (project No. 01201351504); all other studies were funded by RFBR and Government of Krasnoyarsk Territory according to the research project No. 16-44-242099.
Предметные рубрики: CDNA CLONING
EXPRESSION
ENZYME
Ключевые слова (''Своб.индексиров.''): bioluminescence--coelenterazine--bioluminescent reporter--psychrophilic--enzyme--molecular adaptation
Аннотация: The bright bioluminescence of copepod Metridia longa is conditioned by a small secreted coelenterazinedependent luciferase (MLuc). To date, three isoforms of MLuc differing in length, sequences, and some properties were cloned and successfully applied as high sensitive bioluminescent reporters. In this work, we report cloning of a novel group of genes from M. longa encoding extremely psychrophilic isoforms of MLuc (MLuc2-type). The novel isoforms share only similar to 54-64% of protein sequence identity with the previously cloned isoforms and, consequently, are the product of a separate group of paralogous genes. The MLuc2 isoform with consensus sequence was produced as a natively folded protein using baculovirus/ insect cell expression system, purified, and characterized. The MLuc2 displays a very high bioluminescent activity and high thermostability similar to those of the previously characterized M. longa luciferase isoform MLuc7. However, in contrast to MLuc7 revealing the highest activity at 12-17 degrees C and 0.5 M NaCl, the bioluminescence optima of MLuc2 isoforms are at similar to 5 degrees C and 1 M NaCl. The MLuc(2) adaptation to cold is also accompanied by decrease of melting temperature and affinity to substrate suggesting a more conformational flexibility of a protein structure. The luciferase isoforms with different temperature optima may provide adaptability of the M. longa bioluminescence to the changes of water temperature during diurnal vertical migrations. (C) 2016 Elsevier Inc. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Burakova L. P., Natashin P. V., Markova S. V., Eremeeva E. V., Malikova N. P., Cheng C., Liu Z. -J., Vysotski E. S.
Заглавие : Mitrocomin from the jellyfish Mitrocoma cellularia with deleted C-terminal tyrosine reveals a higher bioluminescence activity compared to wild type photoprotein
Место публикации : J. Photochem. Photobiol. B Biol. - 2016. - Vol. 162. - С. 286-297. - ISSN 10111344 (ISSN) , DOI 10.1016/j.jphotobiol.2016.06.054
Аннотация: The full-length cDNA genes encoding five new isoforms of Ca2 +-regulated photoprotein mitrocomin from a small tissue sample of the outer bell margin containing photocytes of only one specimen of the luminous jellyfish Mitrocoma cellularia were cloned, sequenced, and characterized after their expression in Escherichia coli and subsequent purification. The analysis of cDNA nucleotide sequences encoding mitrocomin isoforms allowed suggestion that two isoforms might be the products of two allelic genes differing in one amino acid residue (64R/Q) whereas other isotypes appear as a result of transcriptional mutations. In addition, the crystal structure of mitrocomin was determined at 1.30 A resolution which expectedly revealed a high similarity with the structures of other hydromedusan photoproteins. Although mitrocomin isoforms reveal a high degree of identity of amino acid sequences, they vary in specific bioluminescence activities. At that, all isotypes displayed the identical bioluminescence spectra (473–474 nm with no shoulder at 400 nm). Fluorescence spectra of Ca2 +-discharged mitrocomins were almost identical to their light emission spectra similar to the case of Ca2 +-discharged aequorin, but different from Ca2 +-discharged obelins and clytin which fluorescence is red-shifted by 25–30 nm from bioluminescence spectra. The main distinction of mitrocomin from other hydromedusan photoproteins is an additional Tyr at the C-terminus. Using site-directed mutagenesis, we showed that this Tyr is not important for bioluminescence because its deletion even increases specific activity and efficiency of apo-mitrocomin conversion into active photoprotein, in contrast to C-terminal Pro of other photoproteins. Since genes in a population generally exist as different isoforms, it makes us anticipate the cloning of even more isoforms of mitrocomin and other hydromedusan photoproteins with different bioluminescence properties. © 2016 Elsevier B.V.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kaskova, Zinaida M., Dorr, Felipe A., Petushkov, Valentin N., Purtov, Konstantin V., Tsarkova, Aleksandra S., Rodionova, Natalja S., Mineev, Konstantin S., Guglya, Elena B., Kotlobay, Alexey, Baleeva, Nadezhda S., Baranov, Mikhail S., Arseniev, Alexander S., Gitelson, Josef I., Lukyanov, Sergey, Suzuki, Yoshiki, Kanie, Shusei, Pinto, Ernani, Di Mascio, Paolo, Waldenmaier, Hans E., Pereira, Tatiana A., Carvalho, Rodrigo P., Oliveira, Anderson G., Oba, Yuichi, Bastos, Erick L., Stevani, Cassius V., Yampolsky, Ilia V.
Заглавие : Mechanism and color modulation of fungal bioluminescence
Колич.характеристики :8 с
Коллективы : Sao Paulo Research Foundation [FAPESP] [10/11578-5, 13/16885-1, 14/14866-2, 13/07914-8, 2012/12663-1]; CEPID Redoxoma [2013/07937-8]; National Council for Scientific and Technological Development (CNPq) [301307/2013-0]; NAP Redoxoma (PRPUSP) [2011.1.9352.1.8]; Japan Society for the Promotion of Science [16K07715]; Chubu University [AII28II M01]; Russian Science Foundation [16-14-00052]
Место публикации : Sci. Adv.: AMER ASSOC ADVANCEMENT SCIENCE, 2017. - Vol. 3, Is. 4. - Ст.e1602847. - ISSN 2375-2548, DOI 10.1126/sciadv.1602847
Примечания : Cited References:40. - This work was supported by the Sao Paulo Research Foundation [FAPESP grants 10/11578-5 (to A.G.O.), 13/16885-1 (to C.V.S.), 14/14866-2 (to E.L.B.), 13/07914-8 (to E.P. and F.A.D.), and 2012/12663-1 (to P.D.M.) and CEPID Redoxoma 2013/07937-8 (to P.D.M.)], the National Council for Scientific and Technological Development (CNPq) [301307/2013-0 (to P.D.M.)], NAP Redoxoma (PRPUSP) [2011.1.9352.1.8. (to P.D.M.)], the Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research (KAKENHI) [grant no. 16K07715 (to Y.O.)], Chubu University [grant AII28II M01 (to Y.O.)], and the Russian Science Foundation (grant 16-14-00052 to all Russian authors).
Предметные рубрики: SINGLET MOLECULAR-OXYGEN
QUANTUM YIELDS
CHEMILUMINESCENCE
Аннотация: Bioluminescent fungi are spread throughout the globe, but details on their mechanism of light emission are still scarce. Usually, the process involves three key components: an oxidizable luciferin substrate, a luciferase enzyme, and a light emitter, typically oxidized luciferin, and called oxyluciferin. We report the structure of fungal oxyluciferin, investigate the mechanism of fungal bioluminescence, and describe theuseof simple synthetic alpha-pyrones as luciferins to produce multicolor enzymatic chemiluminescence. A high-energy endoperoxide is proposed as an intermediate of the oxidation of the native luciferin to the oxyluciferin, which is a pyruvic acid adduct of caffeic acid. Luciferase promiscuity allows the use of simple alpha-pyrones as chemiluminescent substrates.
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Вид документа : Статья из сборника (выпуск монографической серии)
Шифр издания :
Автор(ы) : Rogozin, Denis, Zadereev, Egor, Prokopkin, Igor, Tolomeev, Alexander, Barkhatov, Yuri, Khromechek, Elena, Degermendzhi, Nadezhda, Drobotov, Anton, Degermendzhi, Andrei
Заглавие : Comparative Study of the Stability of Stratification and the Food Web Structure in the Meromictic Lakes Shira and Shunet (South Siberia, Russia)
Колич.характеристики :36 с
Место публикации : Ecol. Stud.: SPRINGER INTERNATIONAL PUBLISHING AG, 2017. - Vol. 228. - С. 89-124. - (Ecological Studies-Analysis and Synthesis). - , DOI 10.1007/978-3-319-49143-1_5
Примечания : Cited References:53
Предметные рубрики: PHOTOTROPHIC SULFUR BACTERIA
VERTICAL STRATIFICATION
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Вид документа : Статья из сборника (выпуск монографической серии)
Шифр издания :
Автор(ы) : Zadereev, Egor S., Gulati, Ramesh D., Camacho, Antonio
Заглавие : Biological and Ecological Features, Trophic Structure and Energy Flow in Meromictic Lakes
Колич.характеристики :26 с
Место публикации : Ecol. Stud.: SPRINGER INTERNATIONAL PUBLISHING AG, 2017. - Vol. 228. - С. 61-86. - (Ecological Studies-Analysis and Synthesis). - , DOI 10.1007/978-3-319-49143-1_4
Примечания : Cited References:98
Предметные рубрики: PHOTOTROPHIC SULFUR BACTERIA
GRADIENT GEL-ELECTROPHORESIS
16S
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Belolipetskii V. M., Degermendzhi A. G., Genova S. N., Rogozin D. Y.
Заглавие : Change in the circulation regime in the stratified saline Lake Shira (Siberia, Republic of Khakassia)
Место публикации : Dokl. Earth Sci.: Maik Nauka-Interperiodica Publishing, 2017. - Vol. 474, Is. 2. - С. 649-652. - ISSN 1028334X (ISSN) , DOI 10.1134/S1028334X17060010
Ключевые слова (''Своб.индексиров.''): arid regions--lakes--mixing--climate scenarios--continental climate--in-situ data--incomplete mixing--simplified mathematical model--strong winds--vertical stratification--vertical structures--reservoirs (water)
Аннотация: The in-situ data on the vertical structure and stability of the vertical stratification of saline Lake Shira over the past decade (2007–2015) are analyzed. Simplified mathematical models have shown that strong wind in the autumn of 2014 together with rather thick ice in the winter of 2015 caused a change in the circulation regime of this water reservoir from meromictic (incomplete mixing) to holomictic (compete mixing). Based on the results obtained, a circulation regime for deep saline lakes located in the continental climate zone, in particular, in the arid zones of Southern Siberia (Khakassia, Transbaikal, and Altai) can be predicted under various climate scenarios of the future. © 2017, Pleiades Publishing, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nemtseva E. V., Lashchuk O. O., Gerasimova M. A.
Заглавие : Similarity of decay-associated spectra for tryptophan fluorescence of proteins with different structures
Место публикации : Biophysics. - 2016. - Vol. 61, Is. 2. - С. 193-199. - ISSN 00063509 (ISSN) , DOI 10.1134/S0006350916020111
Ключевые слова (''Своб.индексиров.''): denaturation--dielectric relaxation--fluorescence lifetime--tertiary protein structure--tryptophan
Аннотация: Tryptophan fluorescence lifetimes were analyzed for three proteins: human serum albumin, bovine serum albumin, and bacterial luciferase, which contain one, two, and seven tryptophan residues, respectively. For all of the proteins, the fluorescence decays were fitted by three lifetimes: ?1 = 6–7 ns, ?2 = 2.0–2.3 ns, and ?3 ? 0.1 ns (the native state), and ?1 = 4.4–4.6 ns, ?2 = 1.7–1.8 ns, and ?3 ? 0.1 ns (the denatured state). Corresponding decay-associated spectra had similar peak wavelengths and spectrum half-widths both in the native state (??1max = 342 nm, ??2max = 328 nm, and ??3max = 315 nm), and in the denatured state (??1max = 350 nm, ??2max= 343 nm, and ??3max= 317 nm). The differences in the steady-state spectra of the studied proteins were accounted for the individual ratio of the lifetime component contributions. The lifetime components were compared with a classification of tryptophan residues in the structure of these proteins within the discrete states model. © 2016, Pleiades Publishing, Inc.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Burakova L. P., Natashin P. V., Malikova N. P., Niu F., Pu M., Vysotski E. S., Liu Z.-J.
Заглавие : All Ca2+-binding loops of light-sensitive ctenophore photoprotein berovin bind magnesium ions: The spatial structure of Mg2 +-loaded apo-berovin
Место публикации : J. Photochem. Photobiol. B Biol. - 2016. - Vol. 154. - С. 57-66. - ISSN 10111344 (ISSN) , DOI 10.1016/j.jphotobiol.2015.11.012
Ключевые слова (''Своб.индексиров.''): aequorin--bioluminescence--calcium--coelenterazine--obelin
Аннотация: Light-sensitive photoprotein berovin accounts for a bright bioluminescence of ctenophore Beroe abyssicola. Berovin is functionally identical to the well-studied Ca2+-regulated photoproteins of jellyfish, however in contrast to those it is extremely sensitive to the visible light. Berovin contains three EF-hand Ca2+-binding sites and consequently belongs to a large family of the EF-hand Ca2+-binding proteins. Here we report the spatial structure of apo-berovin with bound Mg2+ determined at 1.75 A. The magnesium ion is found in each functional EF-hand loop of a photoprotein and coordinated by oxygen atoms donated by the side-chain groups of aspartate, carbonyl groups of the peptide backbone, or hydroxyl group of serine with characteristic oxygen-Mg2+ distances. As oxygen supplied by the side-chain of the twelfth residue of all Ca2+-binding loops participates in the magnesium ion coordination, it was suggested that Ca2+-binding loops of berovin belong to the mixed Ca2+/Mg2+ rather than Ca2+-specific type. In addition, we report an effect of physiological concentration of Mg2+ on bioluminescence of berovin (sensitivity to Ca2+, rapid-mixed kinetics, light-sensitivity, thermostability, and apo-berovin conversion into active protein). The different impact of physiological concentration of Mg2+ on berovin bioluminescence as compared to hydromedusan photoproteins was attributed to different affinities of the Ca2 +-binding sites of these photoproteins to Mg2+. © 2015 Elsevier B.V. All rights reserved.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Trifonov S. V., Kudenko Y. A., Tikhomirov A. A.
Заглавие : Prospects for using a full-scale installation for wet combustion of organic wastes in closed life support systems
Место публикации : Life Sci. Space Res. - 2015. - Vol. 7. - С. 15-21. - ISSN 22145524 (ISSN) , DOI 10.1016/j.lssr.2015.08.003
Ключевые слова (''Своб.индексиров.''): automatic control--human waste--life support system--physicochemical oxidation--radish growth--hydrogen peroxide--alternating current--article--closed life support system--combustion--control system--electric field--electricity--human--microclimate--mineralization--organic waste--oxidation--radish--recycling--scale up--waste--wet combustion
Аннотация: The issue of recycling organic wastes in closed life support systems (CLSS) includes both fundamental aspects of environmental safety of the recycled products and their effective involvement in material cycles and technical aspects related to the structure of the system and the crew's demands. This study estimates the effectiveness of wet combustion of different amounts of organic wastes in hydrogen peroxide under application of an alternating current electric field. The study also addresses the possibility of controlling the process automatically. The results show that processing of greater amounts of wastes reduces specific power consumption and shortens the duration of the process, without significantly affecting the level of oxidation of the products. An automatic control system for a semi-commercial installation has been constructed and tested experimentally. The solution of mineralized human wastes prepared in the automatically controlled process in this installation was successfully used to grow radish plants, with the main production parameters being similar to those of the control. © 2015 The Committee on Space Research (COSPAR). Published by Elsevier Ltd. All rights reserved.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Dubinnyi, Maxim A., Kaskova, Zinaida M., Rodionova, Natalja S., Baranov, Mikhail S., Gorokhovatsky, Andrey Yu., Kotlobay, Alexey, Solntsev, Kyril M., Tsarkova, Aleksandra S., Petushkov, Valentin N., Yampolsky, Ilia V.
Заглавие : Novel Mechanism of Bioluminescence: Oxidative Decarboxylation of a Moiety Adjacent to the Light Emitter of Fridericia Luciferin
Колич.характеристики :3 с
Коллективы : Russian Science Foundation [14-50-00131], President of the Russian Federation; National Science Foundation [CHE-1213047]
Место публикации : Angew. Chem.-Int. Edit.: WILEY-V C H VERLAG GMBH, 2015. - Vol. 54, Is. 24. - С. 7065-7067. - ISSN 1433-7851, DOI 10.1002/anie.201501668. - ISSN 1521-3773(eISSN)
Примечания : Cited References:15. - We thank Dr. K.V. Antonov for acquisition of LC-HRMS spectra and Prof. Gary Schuster for fruitful discussion. This work was supported by the Russian Science Foundation grant 14-50-00131. M.S.B. acknowledges support by a stipend program of the President of the Russian Federation. K.M.S. acknowledges generous support from the National Science Foundation (CHE-1213047). This research was carried out using the equipment provided by IBCH core facility (CKP IBCH).
Предметные рубрики: STRUCTURE ELUCIDATION
CHEMILUMINESCENCE
CYPRIDINA
Ключевые слова (''Своб.индексиров.''): bioluminescence--bioorganic chemistry--luciferin--oxidative--decarboxylation--peptides
Аннотация: A novel luciferin from a bioluminescent Siberian earthworm Fridericia heliota was recently described. In this study, the Fridericia oxyluciferin was isolated and its structure elucidated. The results provide insight into a novel bioluminescence mechanism in nature. Oxidative decarboxylation of a lysine fragment of the luciferin supplies energy for light generation, while a fluorescent CompX moiety remains intact and serves as the light emitter.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vereshchagina T. A., Fedorchak M. A., Sharonova O. M., Fomenko E. V., Shishkina N. N., Zhizhaev A. M., Kudryavtsev A. N., Frank L. A., Anshits A. G.
Заглавие : Ni2+-zeolite/ferrosphere and Ni2+-silica/ferrosphere beads for magnetic affinity separation of histidine-tagged proteins
Место публикации : Dalton Trans. - 2016. - Vol. 45, Is. 4. - С. 1582-1592. - ISSN 14779226 (ISSN) , DOI 10.1039/c5dt03827h
Ключевые слова (''Своб.индексиров.''): alkalinity--amino acids--chemical modification--fly ash--hydrothermal synthesis--ion exchange--magnetic separation--magnetism--proteins--silica--zeolites--conventional methods--core shell structure--green fluorescent protein--histidine-tagged proteins--hydrothermal treatments--magnetic affinity--mesoporous silica--sorption capacities--nickel
Аннотация: Magnetic Ni2+-zeolite/ferrosphere and Ni2+-silica/ferrosphere beads (Ni-ferrosphere beads - NFB) of a core-shell structure were synthesized starting from coal fly ash ferrospheres having diameters in the range of 0.063-0.050 mm. The strategy of NFB fabrication is an oriented chemical modification of the outer surface preserving the magnetic core of parent beads with the formation of micro-mesoporous coverings. Two routes of ferrosphere modification were realized, such as (i) hydrothermal treatment in an alkaline medium resulting in a NaP zeolite layer and (ii) synthesis of micro-mesoporous silica on the glass surface using conventional methods. Immobilization of Ni2+ ions in the siliceous porous shell of the magnetic beads was carried out via (i) the ion exchange of Na+ for Ni2+ in the zeolite layer or (ii) deposition of NiO clusters in the zeolite and silica pores. The final NFB were tested for affinity in magnetic separation of the histidine-tagged green fluorescent protein (GFP) directly from a cell lysate. Results pointed to the high affinity of the magnetic beads towards the protein in the presence of 10 mM EDTA. The sorption capacity of the ferrosphere-based Ni-beads with respect to GFP was in the range 1.5-5.7 mg cm-3. © The Royal Society of Chemistry.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Burakova L. P., Stepanyuk G. A., Eremeeva E. V., Vysotski E. S.
Заглавие : Role of certain amino acid residues of the coelenterazine-binding cavity in bioluminescence of light-sensitive Ca2+-regulated photoprotein berovin
Место публикации : Photochem. Photobiol. Sci. - 2016. - Vol. 15, Is. 5. - С. 691-704. - ISSN 1474905X (ISSN) , DOI 10.1039/c6pp00050a
Аннотация: Bright bioluminescence of ctenophores is caused by Ca2+-regulated photoproteins. Although these photoproteins are functionally identical to and share many properties of cnidarian photoproteins, like aequorin and obelin, and retain the same spatial architecture, they are extremely sensitive to light, i.e. lose the ability to bioluminesce on exposure to light over the entire absorption spectrum. In addition, the degree of identity of their amino acid sequences with those of cnidarian photoproteins is only 29.4%. This suggests that the residues involved in bioluminescence of ctenophore and cnidarian photoproteins significantly differ. Here we describe the bioluminescent properties of berovin mutants with substitution of the residues located in the photoprotein internal cavity. Since the spatial structure of berovin bound with a substrate is not determined yet, to identify these residues we have modeled it with an accommodated substrate using the structures of some cnidarian Ca2+-regulated photoproteins with bound coelenterazine or coelenteramide as templates in order to obtain an adequate sampling and to take into account all possible conformers and variants for ligand-protein docking. Based on the impact of substitutions on the bioluminescent properties and model structures we speculate that within the internal cavity of ctenophore photoproteins, coelenterazine is bound as a 2-peroxy anion adduct which is stabilized owing to Coulomb interaction with a positively charged guanidinium group of Arg41 paired with Tyr204. In this case, the bioluminescence reaction is triggered by only calcium-induced conformational changes leading to the disturbance of charge-charge interaction. © 2016 The Royal Society of Chemistry and Owner Societies.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova, Tatiana, Zhila, Natalia, Vinogradova, Olga, Shumilova, Anna, Prudnikova, Svetlana, Shishatskaya, Ekaterina
Заглавие : Characterization of biodegradable poly-3-hydroxybutyrate films and pellets loaded with the fungicide tebuconazole
Колич.характеристики :12 с
Коллективы : Russian Science Foundation [14-26-00039]
Место публикации : Environ. Sci. Pollut. Res.: SPRINGER HEIDELBERG, 2016. - Vol. 23, Is. 6. - С. 5243-5254. - ISSN 0944-1344, DOI 10.1007/s11356-015-5739-1. - ISSN 1614-7499(eISSN)
Примечания : Cited References:33. - This study was supported by the Russian Science Foundation (grant no. 14-26-00039).
Предметные рубрики: CONTROLLED-RELEASE
MICROBIAL-DEGRADATION
BRASSICA-NAPUS
OILSEED RAPE
Ключевые слова (''Своб.индексиров.''): poly(3-hydroxybutyrate)--tebuconazole--slow-release formulations--controlled release--antifungal activity
Аннотация: Biodegradable polymer poly(3-hydroxybutyrate) (P3HB) has been used as a matrix to construct slow-release formulations of the fungicide tebuconazole (TEB). P3HB/TEB systems constructed as films and pellets have been studied using differential scanning calorimetry, X-ray structure analysis, and Fourier transform infrared spectroscopy. TEB release from the experimental formulations has been studied in aqueous and soil laboratory systems. In the soil with known composition of microbial community, polymer was degraded, and TEB release after 35 days reached 60 and 36 % from films and pellets, respectively. That was 1.23 and 1.8 times more than the amount released to the water after 60 days in a sterile aqueous system. Incubation of P3HB/TEB films and pellets in the soil stimulated development of P3HB-degrading microorganisms of the genera Pseudomonas, Stenotrophomonas, Variovorax, and Streptomyces. Experiments with phytopathogenic fungi F. moniliforme and F. solani showed that the experimental P3HB/TEB formulations had antifungal activity comparable with that of free TEB.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Syromotina D. S., Surmenev R. A., Surmeneva M. A., Boyandin A. N., Nikolaeva E. D., Prymak O., Epple M., Ulbricht M., Oehr C., Volova T. G.
Заглавие : Surface wettability and energy effects on the biological performance of poly-3-hydroxybutyrate films treated with RF plasma
Место публикации : Mater. Sci. Eng. C. - 2016. - Vol. 62. - С. 450-457. - ISSN 09284931 (ISSN) , DOI 10.1016/j.msec.2016.01.075
Ключевые слова (''Своб.индексиров.''): plasma--polar component--poly-3-hydroxybutyrate membranes--surface energy--surface treatment--ammonia--biodegradable polymers--cell adhesion--contact angle--free energy--interfacial energy--plasma applications--plasmas--surface chemistry--surface properties--surface roughness--surface topography--wetting--biological performance--crystalline structure--polar components--poly-3-hydroxybutyrate--surface free energy--surface roughness changes--surface wettability--water contact angle--surface treatment
Аннотация: The surface properties of poly-3-hydroxybutyrate (P3HB) membranes were modified using oxygen and an ammonia radio-frequency (RF, 13.56 MHz) plasma. The plasma treatment procedures used in the study only affected the surface properties, including surface topography, without inducing any significant changes in the crystalline structure of the polymer, with the exception being a power level of 250 W. The wettability of the modified P3HB surfaces was significantly increased after the plasma treatment, irrespective of the treatment procedure used. It was revealed that both surface chemistry and surface roughness changes caused by the plasma treatment affected surface wettability. A treatment-induced surface aging effect was observed and resulted in an increase in the water contact angle and a decrease in the surface free energy. However, the difference in the water contact angle between the polymers that had been treated for 4 weeks and the untreated polymer surfaces was still significant. A dependence between cell adhesion and proliferation and the polar component of the surface energy was revealed. The increase in the polar component after the ammonia plasma modification significantly increased cell adhesion and proliferation on biodegradable polymer surfaces compared to the untreated P3HB and the P3HB modified using an oxygen plasma. © 2016 Elsevier B.V.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kuznetsov, Boris N., Malyar, Yuriy N., Kuznetsova, Svetlana A., Grishechko, Lyudmila I., Kazachenko, Alexander S., Levdansky, Alexander V., Pestunov, Andrey V., Boyandin, Anatoly N., Celzard, Alan
Заглавие : Isolation, Study and Application of Organosolv Lignins
Колич.характеристики :29 с
Место публикации : J. Sib. Fed. Univ.-Chem.: SIBERIAN FEDERAL UNIV, 2016. - Vol. 9, Is. 4. - С. 454-482. - ISSN 1998-2836, DOI 10.17516/1998-2836-2016-9-4-454-482. - ISSN 2313-6049(eISSN)
Примечания : Cited References:137
Предметные рубрики: SIZE-EXCLUSION CHROMATOGRAPHY
MOLECULAR-WEIGHT DISTRIBUTION
Ключевые слова (''Своб.индексиров.''): organosolv lignin--isolation--structure--catalytic depolymerization--molecular weight--application--liquid hydrocarbons--aerogels
Аннотация: The analysis of the literature on the methods of soluble organosolv lignins isolation, their physical-chemical study and on the method of their processing to porous aerogels and liquid hydrocarbons was carried out. A review of the literature allowed us to choice of the most important areas of research. For isolation from wood the soluble lignins free from sulfur the methods of catalytic peroxide delignification at mild conditions (temperature
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shidlovskiy I. P., Shumilova A. A., Shishatskaya E. I., Volova T. G.
Заглавие : Properties of Bacterial Cellulose Composites with Silver Nanoparticles
Место публикации : Biophysics: Pleiades Publishing, 2018. - Vol. 63, Is. 4. - С. 519-525. - ISSN 00063509 (ISSN) , DOI 10.1134/S0006350918040188
Ключевые слова (''Своб.индексиров.''): :bacterial cellulose--antibacterial activity--composites--hydrothermal synthesis--silver nanoparticles
Аннотация: Abstract: Composites of bacterial cellulose, which were synthesized in a culture of the strain of acetic acid bacteria Komagataeibacter xylinus VKPM B-12068, with silver nanoparticles were produced hydrothermally by varying the concentrations of AgNO3 in the medium. The presence of silver in the composites was confirmed by elemental analysis. An increase in the number of silver nanoparticles in the composite from 1.08 to 9.1 wt % (from 0.044 to 0.370 mg/cm2) was shown under increasing AgNO3 concentration in the medium from 0.0001 to 0.01 M. The structure, properties of the surface, and the physicochemical properties of the composites depending on the silver content were investigated using scanning electron microscopy, differential scanning calorimetry, X-ray diffraction, and a water contact-angle measurement system. Using the disk-diffusion method, it was shown that the resulting composites have a pronounced antibacterial activity against pathogenic microflora E. coli, Ps. eruginosa, and St. aureus. © 2018, Pleiades Publishing, Inc.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shershneva, Anna, Murueva, Anastasiya, Nikolaeva, Elena, Shishatskaya, Ekaterina, Volova, Tatiana
Заглавие : Novel spray-dried PHA microparticles for antitumor drug release
Колич.характеристики :12 с
Коллективы : Russian Academy of Sciences [AAAA-A17- 117013050028-8]
Место публикации : Dry. Technol.: TAYLOR & FRANCIS INC, 2018. - Vol. 36, Is. 11. - С. 1387-1398. - ISSN 0737-3937, DOI 10.1080/07373937.2017.1407940. - ISSN 1532-2300(eISSN)
Примечания : Cited References:67. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences: [Project no. AAAA-A17- 117013050028-8].
Предметные рубрики: DRYING METHOD
IN-VITRO
BIODEGRADABLE NANOPARTICLES
ORAL
Ключевые слова (''Своб.индексиров.''): 5-fluorouracil--efficiency--microparticles--paclitaxel--polyhydroxyalkanoates--spray-drying
Аннотация: The production of poly-3-hydroxybutyrate (P3HB) and poly-3-hydroxybutyrate/polyethylene glycol (PEG)-based microparticles, loaded with antitumor drugs paclitaxel (PTX) and 5-Fluorouracil (5-FU) by spray-drying technique, was investigated. The average diameter of microparticles was found to be 3.4 +/- 0.5 pm and zeta potential was about -44 mV. The addition of surfactant PEG did not show any effect on the morphological characteristics of the particles. But the chemical structure of drug influenced on the properties. Microparticles had heterogeneous pores on the surface when the hydrophobic PTX was encapsulated. It was established that the addition of surfactant positively influenced on the properties of particles and led to the loading of 5-FU directly into the matrix. This is confirmed by the results of electron microscopy and dynamics of drug release in vitro. As a whole, the release profiles of PTX and 5-FU from composite P3HB/PEG microparticles were less than from P3HB microparticles. The results of the morphological evaluation of Hela cells demonstrated that the use of cytostatic drugs loaded in P3HB microparticles induces morphological changes associated with apoptosis (chromatin condensation, core fragmentation, margination of nucleus). Thus, the obtained results can serve as the basis for the development of new antitumor drugs of prolonged action, intended for various modes of administration.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Feniova I., Dawidowicz P., Ejsmont-Karabin J., Gladyshev M., Kalinowska K., Karpowicz M., Kostrzewska-Szlakowska I., Majsak N., Petrosyan V., Razlutskij V., Rzepecki M., Sushchik N., Dzialowski A. R.
Заглавие : Effects of zebra mussels on cladoceran communities under eutrophic conditions
Место публикации : Hydrobiologia: Springer International Publishing, 2018. - С. 1-18. - ISSN 00188158 (ISSN) , DOI 10.1007/s10750-018-3699-4
Ключевые слова (''Своб.индексиров.''): chlorophyll--food quality--life-table experiments--phosphorus limitation--zooplankton
Аннотация: The purpose of this study was to determine how zebra mussels affected cladoceran community structure under eutrophic conditions. We conducted a mesocosm study where we manipulated the presence of zebra mussels and the presence of large-bodied Daphnia (Daphnia magna and Daphnia pulicaria). We also conducted a complimentary life-table experiment to determine how water from the zebra mussel treatment affected the life history characteristics of the cladoceran species. We anticipated that small- and large-bodied cladoceran species would respond differently to changes in algal quality and quantity under the effects of zebra mussels. Large-bodied Daphnia successfully established in the zebra mussel treatment but failed to grow in the control. We did not observe positive relationships between food concentrations and cladoceran abundances. However, the phosphorus content in the seston indicated that food quality was below the threshold level for large-bodied cladocerans at the beginning of the experiment. We believe that zebra mussels quickly enhanced the phosphorus content in the seston due to the excretion of inorganic phosphorus, thus facilitating the development of large-bodied Daphnia. In conclusion, our results suggest that zebra mussels can alter the phosphorus content of seston in lakes and this can affect the dynamics of crustacean zooplankton. © 2018 Springer International Publishing AG, part of Springer Nature
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Eremeeva, Elena V., Vysotski, Eugene S.
Заглавие : Exploring Bioluminescence Function of the Ca2+-regulated Photoproteins with Site-directed Mutagenesis
Колич.характеристики :16 с
Коллективы : Russian Foundation for Basic Research [17-04-00764]; Russian Academy of Sciences [0356-2017-0017]
Место публикации : Photochem. Photobiol.: WILEY, 2019. - Vol. 95, Is. 1. - С. 8-23. - ISSN 0031-8655, DOI 10.1111/php.12945. - ISSN 1751-1097(eISSN)
Примечания : Cited References:88. - This work was supported by grant 17-04-00764 of Russian Foundation for Basic Research and the state budgetallocated to the fundamental research at the Russian Academy of Sciences (project 0356-2017-0017).
Предметные рубрики: CALCIUM-BINDING PHOTOPROTEIN
GREEN-FLUORESCENT PROTEIN
JELLYFISH
Ключевые слова (''Своб.индексиров.''): bioluminescence--coelenterazine--aequorin--obelin--clytin--mitrocomin--ef-hand protein
Аннотация: Site-directed mutagenesis is a powerful tool to investigate the structure-function relationship of proteins and a function of certain amino acid residues in catalytic conversion of substrates during enzymatic reactions. Hence, it is not surprising that this approach was repeatedly applied to elucidate the role of certain amino acid residues in various aspects of photoprotein bioluminescence, mostly for aequorin and obelin, and to design mutant photoproteins with altered properties (modified calcium affinity, faster or slower bioluminescence kinetics, different emission color) which would either allow the development of novel bioluminescent assays or improvement of characteristics of the already existing ones. This information, however, is scattered over different articles. In this review, we systematize the findings that were made using site-directed mutagenesis studies regarding the impact of various amino acid residues on bioluminescence of hydromedusan Ca2+-regulated photoproteins. All key residues that have been identified are pinpointed, and their influence on different aspects of photoprotein functioning such as active photoprotein complex formation, bioluminescence reaction, calcium response and light emitter formation is discussed.
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