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^a314.27.17^2VINITI
Ч-25

Частичная реконструкция in vitro люминесцентной системы светящихся бактерий [Текст] : научное издание / В. В. Межевикин [и др.] // Микробиология. - 1985. - Т. 54, N 2. - С. 209-213 . - ISSN 0026-3656

ГРНТИ

31.27.17

РУБ 314.27.17
Рубрики:
PHOTOBACTERIUM LEIOGNATHI
   БАКТЕРИИ
   СВЕТЯЩИЕСЯ
   МЕТАБОЛИЧЕСКАЯ ОРГАНИЗАЦИЯ
   ЛЮМИНЕСЦЕНТНАЯ СИСТЕМА
   LUMINESCENT BACTERIUM SYSTEM
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Межевикин, В.В.; Высоцкий, Е.С.; Заворуев, В.В.; Кузнецов, П.В.; Райбекас, А.А.

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^a621.39.51^2VINITI
М 59

Микробные тест-системы для оценки степени токсичности химических соединений [Текст] : научное издание / Л. Ю. Попова [и др.] ; Академия наук СССР. Сибирское отделение. Институт биофизики, АН СССР. СО. Ин-т биофиз. // Препр. - 1991. - N 163. - С. 1-33

ГРНТИ

62.39.51

РУБ 621.39.51
Рубрики:
БИОСЕНСОРЫ
   МИКРООРГАНИЗМЫ
   ТЕСТ-СИСТЕМЫ
   СПЕЦИФИЧНОСТЬ
   ЧУВСТВИТЕЛЬНОСТЬ
   МИКОТОКСИНЫ
   ФЕНОЛЫ
   НИТРИТЫ
   ИНСЕКТИЦИДЫ
   ТОКСИЧНЫЕ СОЕДИНЕНИЯ
   ОПРЕДЕЛЕНИЕ
   СТОЧНЫЕ ВОДЫ
   БИОЛЮМИНЕСЦЕНЦИЯ
   ОБЗОРЫ
   БИБЛ 51
   TEST-SYSTEM
   MICROORGANISMS
   BIOSENSOR
   TOXIC SUBSTANCES
Аннотация: Анализируются возможности использования микробных тестов-систем для оценки токсичности химических соединений. Подробно описан биосенсор для определения фенолов на основе иммобилизованных клеток микроорганизмов. Рассматриваются пути повышения специфичности и чувствительности бактериальных биолюминесцентных катодов на конкертных примерах. Показано, что, выбирая оптимальные условия культивирования и обработки тест-объекта, можно повысить порог чувствительности к фенолам, нитритам и инсектицидам. Перспективен также селекционный подход, основанный на подборе яркосветящихся вариантов в популяции исследуемого штамма бактерий, имеющих более высокую чувствительность, которая не зависит от условий культивирования. Библ. 51
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Попова, Л.Ю.; Луцкая, Н.И.; Жуков, А.Г.; Брильков, А.В.; Печуркин, Н.С.; Академия наук СССР. Сибирское отделение. Институт биофизики

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^a621.09.39^2VINITI
И 88

Использование светящихся бактерий для создания тест-системы на гексахлоранциклогексан [Текст] : научное издание / Л. Ю. Попова [и др.] // Прикл. биохимия и микробиол. - 1991. - Т. 27, N 6. - С. 905-910 . - ISSN 0555-1099

ГРНТИ

62.09.39

РУБ 621.09.39
Рубрики:
БАКТЕРИИ
   СВЕТЯЩИЕСЯ
   ИСПОЛЬЗОВАНИЕ
   ГЕКСАХЛОРАНЦИКЛОГЕКСАН
   СОЗДАНИЕ ТЕСТ-СИСТЕМЫ
   TEST SYSTEM
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Попова, Л.Ю.; Медведева, С.Е.; Могильная, О.А.; Пузырь, А.П.; Печуркин, Н.С.

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Wheat growth on neutral and soil-like substrates: Carbon dioxide exchange and microflora / J. -B. Gros [et al.] // Acta Horticulturae. - 2004. - Vol. 644. - P243-248
Кл.слова (ненормированные):
Carbon dioxide -- Closed ecological system
Аннотация: The soil-like substrate (SLS), which is a potential candidate for employment in closed ecological systems, has been tested. CO2 exchange, composition and numbers of soil microflora have been examined in .wheat-SLS. system. The results produced have been compared to analogous characteristics of .wheat-neutral substrate. system. A hydroponic method was used under wheat growing on the neutral substrate (expanded clay aggregate). Plants for both studies were grown in closed environment from seed to physiological maturity. In the .wheat-SLS. system, the net photosynthetic rate of canopy was positive in the course of 6.55 days after planting. The net photosynthetic rate of canopy in .wheat-neutral substrate. system was positive in the entire course of vegetation. According to calculations in the course of vegetation, photosynthesis has withdrawn 3.28 kg m-2 CO2 from the .wheat-SLS. system and 3.40 kg m-2 CO2 from the .wheat-neutral substrate. system. On the SLS dominant among bacteria were the spore-forming bacteria from Bacillus genus, among fungi . from Trichoderma genus. In the hydroponic cultivation on neutral substrate dominant were bacteria from Pseudomonas genus, most commonly found fungi were species from Fusarium and Botrytis genera.

Scopus
Держатели документа:
LGCB, Universte B. Pascal, BP206, FR-63174 Aubiere cedex, France
ESA, Estec 2200 AG Noordwijk, Netherlands
Academgorodok, Institute of Biophysics, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gros, J.-B.; Lasseur, C.; Tikhomirov, A.A.; Manukovsky, N.S.; Ushakova, S.A.; Zolotukhin, I.G.; Tirranen, L.S.; Borodina, E.V.; Kovalev, V.S.

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Waste bioregeneration in life support CES: Development of soil organic substrate [Text] / N. S. Manukovsky [et al.] ; ed. RM Wheeler [et al.] // LIFE SCIENCES: LIFE SUPPORT SYSTEMS STUDIES-I. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON PRESS LTD, 1997. - Vol. 20: F4.6, F4.8, F4.2 and F4.9 Symposia of COSPAR Scientific Commission F on Life Sciences - Life Support System Studies-I, at the 31st COSPAR Scientific Assembly (JUL 14-SEP 21, 1996, BIRMINGHAM, ENGLAND), Is. 10. - P. 1827-1832, DOI 10.1016/S0273-1177(97)00848-X. - Cited References: 13 . - ISBN 0273-1177. - ISBN 0-08-043307-3

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences

Аннотация: An experimental model of matter turnover in the biotic cycle: plants (plant biomass) -- mushrooms (residual substrate + mushroom fruit bodies) -- worms (biohumus) -- microorganisms (soil-like substrate) -- plants is presented. The initial mass of soillike substrate was produced from wheat plants grown in a hydroponic system, Three cycles of matter turnover in the biotic cycle were carried out. Grain productivity on soillike substrate was 21.87 g/m(2) day(1). The results obtained were used for designing a CES containing man, plants, soillike substrate, bioregeneration module and aquaculture. It was shown, that the circulating dry mass of the CES is 756 kg. The main part (88%) of the circulating mass accumulates in the soillike substrate and bioregeneration module. (C) 1997 COSPAR. Published by Elsevier Science Ltd.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Manukovsky, N.S.; Kovalev, V.S.; Rygalov, V.Y.; Zolotukhin, I.G.; Wheeler, RM \ed.\; Garland, JL \ed.\; Tibbitts, TW \ed.\; Nielsen, SS \ed.\

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Volatile metabolites of higher plant crops as a photosynthesizing life support system component under temperature stress at different light intensities / I. I. Gitelson [et al.] // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1781-1786, DOI 10.1016/S0273-1177(03)00121-2 . - ISSN 0273-1177
Кл.слова (ненормированные):
Composition -- Crops -- Heat resistance -- Metabolites -- Photosynthesis -- Volatile metabolites -- Space research -- biosphere -- article -- comparative study -- gas -- growth, development and aging -- heat -- indoor air pollution -- light -- metabolism -- microclimate -- photon -- photosynthesis -- physiology -- radiation exposure -- volatilization -- wheat -- Air Pollution, Indoor -- Environment, Controlled -- Gases -- Heat -- Life Support Systems -- Light -- Photons -- Photosynthesis -- Triticum -- Volatilization
Аннотация: The effect of elevated temperatures of 35 and 45В°C (at the intensities of photosynthetically active radiation 322, 690 and 1104 ?mol-m-2-s-1) on the photosynthesis, respiration, and qualitative and quantitative composition of the volatiles emitted by wheat (Triticum aestuvi L., cultivar 232) crops was investigated in growth chambers. Identification and quantification of more than 20 volatile compounds (terpenoids - ?-pinene, ?3 carene, limonene, benzene, ?-and transcaryophyllene, ?- and ?-terpinene, their derivatives, aromatic hydrocarbons, etc.) were conducted by gas chromatograph/mass spectrometry. Under light intensity of 1104 ?mol-m-2-s-1, heat resistance of photosynthesis and respiration increased at 35В°C and decreased at 45В°C. The action of elevated temperatures brought about variations in the rate and direction of the synthesis of volatile metabolites. The emission of volatile compounds was the greatest under a reduced irradiation of 322 ?mol-m-2-s-1 and the smallest under 1104 ?mol-m-2-s-1 at 35В°C. During the repair period, the contents and proportions of volatile compounds were different from their initial values, too. The degree of disruption and the following recovery of the functional state depended on the light intensity during the exposure to elevated temperatures. The investigation of the atmosphere of the growth chamber without plants has revaled the substances that were definitely technogenic in origin: tetramethylurea, dimethylsulfide, dibutylsulfide, dibutylphthalate, and a number of components of furan and silane nature. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, I.I.; Tikhomirov, A.A.; Parshina, O.V.; Ushakova, S.A.; Kalacheva, G.S.

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Using DCMU-fluorescence method for the identification of dominant phytoplankton groups [Text] / N. A. Gaevsky [et al.] // J. Appl. Phycol. - 2005. - Vol. 17, Is. 6. - P. 483-494, DOI 10.1007/s10811-005-2903-x. - Cited References: 28 . - ISSN 0921-8971

РУБ Biotechnology & Applied Microbiology + Marine & Freshwater Biology
Рубрики:
IN-VIVO
   CHLOROPHYLL FLUORESCENCE
   FLUOROMETRIC METHOD
   SITU
   PHOTOSYNTHESIS
   CYANOBACTERIA
   ALGAE
   EVOLUTION
   CULTURES
   LAKES
Кл.слова (ненормированные):
algae -- chlorophyll -- fluorescence -- vizualization
Аннотация: For the identification of ecologically significant dominant groups of phytoplanktonic algae a polychromatic DCMU-induced fluorescence method is recommended. A special fluorometer equipped with a system of replaceable filters is used to differentiate three regions of the spectrum (410 +/- 20, 510 +/- 20 and 540 +/- 10 nm) that can excite the basic light-harvesting pigments. Total and differential (for every algal taxon studied) chlorophyll a calculated from the fluorescence signals is in good agreement with biomass estimates from direct cell counts for several different trophic types of aquatic systems. This is made possible by the vizualization of the ratios of fluorescence signal values in their own coordinates: first, to decide whether it is necessary to correct linear equations in order to eliminate negative solutions; second, to determine the possibility of nulling the negative solution if a point is situated close to a side of the triangle; and third, to reduce the number of linear algebraic equations to two if the points are situated along one of the triangle sides or to one if the points are gathered at the apex. The polychromatic DCMU-induced fluorescence method can be used for monitoring natural phytoplankton populations to detect changes in their taxonomic structure.

WOS
Держатели документа:
Krasnoyarsk State Univ, Dept Biol, Krasnoyarsk, Russia
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gaevsky, N.A.; Kolmakov, V.I.; Anishchenko, O.V.; Gorbaneva, T.B.

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Use of proZZ-obelin fusion protein in bioluminescent immunoassay [Text] / L. A. Frank, V. A. Illarionova, E. S. Vysotski // Biochem. Biophys. Res. Commun. - 1996. - Vol. 219, Is. 2. - P475-479, DOI 10.1006/bbrc.1996.0258. - Cited References: 21 . - 5. - ISSN 0006-291X

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
ESCHERICHIA-COLI
   EXPRESSION
   AEQUORIN
   PURIFICATION
   SYSTEM
Аннотация: Obelin is a photoprotein that emits light by Ca2+-binding. To develop a bioluminescent immunoassay based on the light emission property of obelin, we have expressed the apoobelin fusion protein with ZZ-domain of S. aureus protein A in E. coil by recombinant DNA techniques. The pro2Z-obelin expressed was purified by one-step affinity chromatography on IgG-Agarose. The purified proZZ-obelin has both the luminescent activity of obelin and the IgG-binding ability of ZZ-domain. The specific activity of fusion protein was 8.5 x 10(15) photons per mg of protein. (C) 1996 Academic Press, Inc.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Frank, L.A.; Illarionova, V.A.; Vysotski, E.S.

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Use of human wastes oxidized to different degrees in cultivation of higher plants on the soil-like substrate intended for closed ecosystems / A. A. Tikhomirov [et al.] // Advances in Space Research. - 2010. - Vol. 46, Is. 6. - P744-750, DOI 10.1016/j.asr.2010.02.024 . - ISSN 0273-1177
Кл.слова (ненормированные):
Life support systems -- Microflora -- Mineralized human wastes -- Phototrophic unit -- Wet incineration -- Alternating electromagnetic field -- Bioregenerative life support systems -- Degree of oxidations -- Denitrifying microorganisms -- Growth and development -- Higher plants -- Human waste -- Life support systems -- Mass exchange -- Microbiotas -- Microflora -- Microscopic fungi -- Mineralized human wastes -- Nutrient solution -- Oxidation level -- Phytopathogenic bacteria -- Plant productivity -- Soil-like substrate -- Wheat plants -- Biomolecules -- Electromagnetic fields -- Irrigation -- Magnetic field effects -- Metabolism -- Metabolites -- Oxidation -- Plants (botany) -- Soils -- Solvent extraction -- Wastes -- Waste incineration
Аннотация: To close mass exchange loops in bioregenerative life support systems more efficiently, researchers of the Institute of Biophysics SB RAS (Krasnoyarsk, Russia) have developed a procedure of wet combustion of human wastes and inedible parts of plants using H2O2 in alternating electromagnetic field. Human wastes pretreated in this way can be used as nutrient solutions to grow plants in the phototrophic unit of the LSS. The purpose of this study was to explore the possibilities of using human wastes oxidized to different degrees to grow plants cultivated on the soil-like substrate (SLS). The treated human wastes were analyzed to test their sterility. Then we investigated the effects produced by human wastes oxidized to different degrees on growth and development of wheat plants and on the composition of microflora in the SLS. The irrigation solution contained water, substances extracted from the substrate, and certain amounts of the mineralized human wastes. The experiments showed that the human wastes oxidized using reduced amounts of 30% H2O2: 1 ml/g of feces and 0.25 ml/ml of urine were still sterile. The experiments with wheat plants grown on the SLS and irrigated by the solution containing treated human wastes in the amount simulating 1/6 of the daily diet of a human showed that the degree of oxidation of human wastes did not significantly affect plant productivity. On the other hand, the composition of the microbiota of irrigation solutions was affected by the oxidation level of the added metabolites. In the solutions supplemented with partially oxidized metabolites yeast-like microscopic fungi were 20 times more abundant than in the solutions containing fully oxidized metabolites. Moreover, in the solutions containing incompletely oxidized human wastes the amounts of phytopathogenic bacteria and denitrifying microorganisms were larger. Thus, insufficiently oxidized sterile human wastes added to the irrigation solutions significantly affect the composition of the microbiological component of these solutions, which can ultimately unbalance the system as a whole. В© 2010 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, Akademgorodok, 50/50, Krasnoyarsk 660036, Russian Federation
Universite Blaise Pascal, 24 avenue des Landais, 63174 Aubiere cedex, France
ESA/ESTEC, Keplerlaan 1, 2201 AZ Noordwijk, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Kudenko, Y.A.; Ushakova, S.A.; Tirranen, L.S.; Gribovskaya, I.A.; Gros, J.-B.; Lasseur, C.

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10.

Use of halophytic plants for recycling NaCl in human liquid waste in a bioregenerative life support system / Y. Balnokin [et al.] // Advances in Space Research. - 2010. - Vol. 46, Is. 6. - P768-774, DOI 10.1016/j.asr.2010.03.020 . - ISSN 0273-1177
Кл.слова (ненормированные):
Biological life support system -- Halophytes -- Human waste recycling -- NaCl recycling -- A plants -- Biological life support systems -- Biomass productions -- Bioregenerative life support systems -- Daily ration -- Europaea -- Growth conditions -- Halophytes -- Halophytic plants -- Human waste -- Liquid wastes -- Nutrient solution -- Optimal conditions -- Salicornia europaea -- Body fluids -- Liquids -- Nutrients -- Plant shutdowns -- Plants (botany) -- Recycling -- Sodium alloys -- Sodium chloride
Аннотация: The purpose of this work was to develop technology for recycling NaCl containing in human liquid waste as intrasystem matter in a bioregenerative life support system (BLSS). The circulation of Na+ and Cl- excreted in urine is achieved by inclusion of halophytes, i.e. plants that naturally inhabit salt-rich soils and accumulate NaCl in their organs. A model of Na+ and Cl- recycling in a BLSS was designed, based on the NaCl turnover in the human-urine-nutrient solution-halophytic plant-human cycle. The study consisted of (i) selecting a halophyte suitable for inclusion in a BLSS, and (ii) determining growth conditions supporting maximal Na + and Cl- accumulation in the shoots of the halophyte growing in a nutrient solution simulating mineralized urine. For the selected halophytic plant, Salicornia europaea, growth rate under optimal conditions, biomass production and quantities of Na+ and Cl- absorbed were determined. Characteristics of a plant production conveyor consisting of S. europaea at various ages, and allowing continuity of Na+ and Cl - turnover, were estimated. It was shown that closure of the NaCl cycle in a BLSS can be attained if the daily ration of fresh Salicornia biomass for a BLSS inhabitant is approximately 360 g. В© 2010 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
K.A. Timiryazev Plant Physiology Institute, Russian Academy of Sciences, 127276 Moscow, Russian Federation
Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, 660036 Krasnoyarsk, Russian Federation
TEC-MCT, ESA/Estec, 1 Keplerlaan, 2201 AG Noordwijk, Netherlands
Universite Blaise Pascal, LGCB, Polytech'Clermont-Ferrand, BP206, 63174 Aubire cedex, France : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Balnokin, Y.; Nikolai, M.; Popova, L.; Tikhomirov, A.; Ushakova, S.; Lasseur, C.; Gros, J.-B.

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11.

Use of bioluminescent enzyme system to detect antioxidant activity of fullerenol C60Oy(OH)(x) [Text] / A. . Tarasova [et al.] // Luminescence. - 2014. - Vol. 29. - P100-101. - Cited References: 6 . - ISSN 1522-7235. - ISSN 1522-7243

WOS
Держатели документа:
[Tarasova, Anna
Kovel, Ekaterina] Siberian Fed Univ, Krasnoyarsk, Russia
[Tarasova, Anna
Kudryasheva, Nadezhda] Inst Biophys SB RAS, Krasnoyarsk, Russia
[Churilov, Grigoriy
Vnukova, Natalia
Isakova, Victoria
Osipova, Irina] Inst Phys SB RAS, Krasnoyarsk, Russia
ИБФ СО РАН
ИФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tarasova, A...; Kudryasheva, N...; Kovel, E...; Churilov, G...; Vnukova, N...; Isakova, V...; Osipova, I...

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12.

Unique diversity of radioactive particles found in the Yenisei River floodplain / A. Bolsunovsky [et al.] // Sci Rep. - 2017. - Vol. 7. - Ст. 11132, DOI 10.1038/s41598-017-11557-7. - Cited References:22. - The authors are grateful to researchers of the Institute of Biophysics FRC KSC SB RAS (Krasnoyarsk, Russia) and the Institute of Geology and Mineralogy SB RAS (Novosibirsk, Russia) for their assistance in isolation and analysis of radioactive particles. Part of the work is also supported by the Research Council of Norway through its Centres of Excellence funding scheme, project number 223268/F50. Investigations of the particles were partially supported by IAEA (Project 17941). . - ISSN 2045-2322

РУБ Multidisciplinary Sciences
Рубрики:
CONTAINING CONTROL RODS
   TRANSURANIC ELEMENTS
   BOTTOM SEDIMENTS
   HOT
Аннотация: The long-term operation of three reactors and the radiochemical plant of the Mining-and-Chemical Combine (MCC), Russia's largest producer of weapons-grade plutonium, has resulted in radioactive contamination of the Yenisei River floodplain. From 1995 to 2016, we found more than 200 radioactive particles (RP) in the Yenisei floodplain, downstream of the MCC. Analytical characterization showed that most of the RP were fuel particles, which were carried into the river after incidents at the MCC reactors. Having compared the Cs-137/Cs-134 ratios in the particles, we determined three time intervals when the RP were formed. The plutonium isotope ratios (Pu-238/Pu-239,Pu-240) vary substantially between the particles and indicate several different source terms. In addition to fuel RP, we found particles that only contained activation products (Co-60 or europium isotopes). SEM and gamma-spectrometry showed that the cobalt particles could have originated from the corrosion of the reactor coolant system and the europium particles -from the damaged compensating rods. No europium particles have been found anywhere else in the world. The presence of RP from different sources (fuel, cobalt, and europium particles) in the Yenisei River floodplain makes this region a unique site for studying environmental effects of the particles. These RP represent point sources of radioecological significance.

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Смотреть статью
Держатели документа:
FRC Krasnoyarsk Sci Ctr SB RAS, Inst Biophys SB RAS, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Inst Geol & Mineral SB, Novosibirsk 630090, Russia.
Norwegian Univ Life Sci, Ctr Environm Radioact, As, Norway.

Доп.точки доступа:
Bolsunovsky, Alexander; Melgunov, Mikhail; Chuguevskii, Alexey; Lind, Ole Christian; Salbu, Brit; Research Council of Norway through its Centres of Excellence funding scheme [223268/F50]; IAEA [17941]

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13.

Ultrastructure and morphology of the bioluminescent system in "bioluminescent" and "non-bioluminescent" scale-worms (Polychaeta, Polynoidae) [Text] / N. B. Aneli [et al.] // Luminescence. - 2014. - Vol. 29. - P41-42. - Cited References: 5 . - ISSN 1522-7235. - ISSN 1522-7243
Рубрики:
ORGANS

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Держатели документа:
[Aneli, N. B.] Russian Acad Sci EZAN, Expt Factory Sci Engn, Chernogolovka, Moscow Region, Russia
[Belousova, A. A.] Moscow MV Lomonosov State Univ, Fac Biol, Dept Invertebrate Zool, Moscow, Russia
[Saprunova, V. B.] Moscow MV Lomonosov State Univ, AN Belozersky Inst Physicochem Biol, Moscow, Russia
[Petushkov, V. N.] Russian Acad Sci, Siberian Branch, Lab Photobiol, Inst Biophys, Krasnoyarsk, Russia
[Kondrashov, F. A.
Plyuscheva, M. V.] CRG, Barcelona, Spain
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Aneli, N.B.; Belousova, A.A.; Saprunova, V.B.; Petushkov, V.N.; Kondrashov, F.A.; Plyuscheva, M.V.

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14.

Two new types of bioluminescent systems of soil enchytraeids (Annelida:Clitellata:Oligochaeta:Enchytraeidae) / V. N. Petushkov, N. S. Rodionova // Doklady Akademii Nauk. - 2005. - Vol. 401, Is. 2. - С. 263-266 . - ISSN 0869-5652
Кл.слова (ненормированные):
Analysis -- Luminescence -- Models -- Soils -- Bioluminescent systems -- Oligochaeta earth-worm -- Biology
Аннотация: For the first time comparative data on localization, structural-functional organization, optimal functioning conditions for two bioluminescent systems of soil enchytraeids Fridericia heliota and Henlea sp. are given. Strong difference of these systems from each other and from all known among Oligochaeta class is shown. The analysis allows stating the discovery of two new systems of bioluminescent systems of enchytraeids, and accounting peroxide-dependent system of Diplocardia longa megascolecid, establishing multiplicity of luminescent mechanisms for Oligochaeta earth-worm.

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Держатели документа:
Inst. Biofiziki SO RAN, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V.N.; Rodionova, N.S.

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15.

Two forms of substrate for the bioluminescent reaction in three species of basidiomycetes / A. P. Puzyr [et al.] // Mycol. - 2019. - Vol. 10, Is. 2. - P84-91, DOI 10.1080/21501203.2019.1583688 . - ISSN 2150-1203
Кл.слова (ненормированные):
Cold and hot extracts -- culture liquid -- enzymatic system -- hispidin -- luminous fungi -- substrate of luminescent reaction
Аннотация: The luminescent response of the enzymatic system of Armillaria borealis on the cold and hot extracts from cell-free culture liquids of Inonotus obliquus, Pholiota sp. and A. borealis was examined. The greatest influence on the light emission produced by the luminescent system of A. borealis was provided by the temperature at which the probes were prepared for assay. Boiling a culture liquid on water bath for a few minutes promoted a multifold increase in the luminescence. The results of luminescence assay suggest that the substance involved in the bioluminescent reaction in higher fungi is presented in culture liquids and mycelia in two forms. In one form, it is ready to interact with the enzymatic system and in the second form, it becomes accessible for the reaction after heat treatment. The pool of thermoactivated substance was found to be much large than the amount of the ready accessible one. We suggest that predecessors of hispidin, which is fungal luciferin precursor, are responsible for this phenomenon. They are not involved in bioluminescence at their original state and are converted into the substrate under the influence of high temperature. © 2019, © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

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Держатели документа:
Institute of Biophysics, Siberian Branch of Russian Academy of Science, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
Institute of Computational Technologies, Siberian Branch of Russian Academy of Science, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Puzyr, A. P.; Burov, A. E.; Medvedeva, S. E.; Burova, O. G.; Bondar, V. S.

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16.

Trace elements exchange in experimental closed life support systems [Text] / I. V. Gribovskaya, V. Y. Rygalov ; ed. ya, IV Gribovs // SIXTH EUROPEAN SYMPOSIUM ON SPACE ENVIRONMENTAL CONTROL SYSTEMS, VOLS 1 AND 2. Ser. ESA SPECIAL PUBLICATIONS : EUROPEAN SPACE AGENCY, 1997. - Vol. 400: 6th European Symposium on Space Environmental Control Systems (MAY 20-22, 1997, NOORDWIJK, NETHERLANDS). - P. 859-862. - Cited References: 0 . - ISBN 0379-6566. - ISBN 92-9092-283-4

РУБ Engineering, Aerospace

Аннотация: Measurements of trace elements incoming and exiting ''Bios-3'' biological-engineering life support system evidence presence of a source of these elements inside the system. This source is, most probably, structural units (steel walls, hose rubber, plastic coatings), catalysts of the thermal catalytic incinerator, expanded clay aggregate mechanically fixing plants and so on. The trace elements are received by the nutrient solutions and build up in the plant biomass. As a consequence the crew may receive elevated doses of such trace elements as aluminum, lead, nickel, chromium. To reduce income of trace elements to LSS requires thorough selection and preliminary treatment of structural materials with reduced desorption capacities and running-in of the system in idle mode prior to exploitation. This mode involves operation of the engineering part of the system (without plants and humans, but with nutrient solutions) for several months.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gribovskaya, I.V.; Rygalov, V.Y.; Gribovs, ya, IV \ed.\

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17.

Toxicity and antioxidant activity of fullerenol c60,70 with low number of oxygen substituents / E. S. Kovel, A. G. Kicheeva, N. G. Vnukova [et al.] // Int. J. Mol. Sci. - 2021. - Vol. 22, Is. 12. - Ст. 6382, DOI 10.3390/ijms22126382 . - ISSN 1661-6596
Кл.слова (ненормированные):
Antioxidant activity -- Bioluminescent assay -- Fullerenol -- Hormesis -- Reactive oxygen species -- Toxicity
Аннотация: Fullerene is a nanosized carbon structure with potential drug delivery applications. We studied the bioeffects of a water-soluble fullerene derivative, fullerenol, with 10-12 oxygen groups (F10-12); its structure was characterized by IR and XPS spectroscopy. A bioluminescent enzyme system was used to study toxic and antioxidant effects of F10-12 at the enzymatic level. Antioxidant characteristics of F10-12 were revealed in model solutions of organic and inorganic oxidizers. Low-concentration activation of bioluminescence was validated statistically in oxidizer solutions. Toxic and antioxidant characteristics of F10-12 were compared to those of homologous fullerenols with a higher number of oxygen groups:F24-28 and F40-42. No simple dependency was found between the toxic/antioxidant characteristics and the number of oxygen groups on the fullerene’s carbon cage. Lower toxicity and higher antioxidant activity of F24-28 were identified and presumptively attributed to its higher solubility. An active role of reactive oxygen species (ROS) in the bioeffects of F10-12 was demonstrated. Correlations between toxic/antioxidant characteristics of F10-12 and ROS content were evaluated. Toxic and antioxidant effects were related to the decrease in ROS content in the enzyme solutions. Our results reveal a complexity of ROS effects in the enzymatic assay system. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, FRC KSC SB RAS, Krasnoyarsk, 660036, Russian Federation
Institute of Physics SB RAS, FRC KSC SB RAS, Krasnoyarsk, 660036, Russian Federation
FRC KSC SB RAS, Krasnoyarsk, 660036, Russian Federation
Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, 660041, Russian Federation

Доп.точки доступа:
Kovel, E. S.; Kicheeva, A. G.; Vnukova, N. G.; Churilov, G. N.; Stepin, E. A.; Kudryasheva, N. S.

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18.

Toxicity and Antioxidant Activity of Fullerenol C-60,C-70 with Low Number of Oxygen Substituents / E. S. Kovel, A. G. Kicheeva, N. G. Vnukova [et al.] // Int. J. Mol. Sci. - 2021. - Vol. 22, Is. 12. - Ст. 6382, DOI 10.3390/ijms22126382. - Cited References:93. - This research was funded by RFBR, N18-29-19003; RFBR, Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science, N20-44-243001; and partly supported by the Program of the Federal Service for Surveillance on Consumer Rights Protection and HumanWellbeing, Fundamental Study 2020-2025 (Russian Federation). . - ISSN 1422-0067

РУБ Biochemistry & Molecular Biology + Chemistry, Multidisciplinary
Рубрики:
HUMIC SUBSTANCES
DETOXIFICATION PROCESSES
BIOLOGICAL-ACTIVITY
Кл.слова (ненормированные):
fullerenol -- toxicity -- antioxidant activity -- reactive oxygen species -- bioluminescent assay -- hormesis
Аннотация: Fullerene is a nanosized carbon structure with potential drug delivery applications. We studied the bioeffects of a water-soluble fullerene derivative, fullerenol, with 10-12 oxygen groups (F10-12); its structure was characterized by IR and XPS spectroscopy. A bioluminescent enzyme system was used to study toxic and antioxidant effects of F10-12 at the enzymatic level. Antioxidant characteristics of F10-12 were revealed in model solutions of organic and inorganic oxidizers. Low-concentration activation of bioluminescence was validated statistically in oxidizer solutions. Toxic and antioxidant characteristics of F10-12 were compared to those of homologous fullerenols with a higher number of oxygen groups:F24-28 and F40-42. No simple dependency was found between the toxic/antioxidant characteristics and the number of oxygen groups on the fullerene's carbon cage. Lower toxicity and higher antioxidant activity of F24-28 were identified and presumptively attributed to its higher solubility. An active role of reactive oxygen species (ROS) in the bioeffects of F10-12 was demonstrated. Correlations between toxic/antioxidant characteristics of F10-12 and ROS content were evaluated. Toxic and antioxidant effects were related to the decrease in ROS content in the enzyme solutions. Our results reveal a complexity of ROS effects in the enzymatic assay system.

WOS
Держатели документа:
FRC KSC SB RAS, Inst Biophys SB RAS, Krasnoyarsk 660036, Russia.
FRC KSC SB RAS, Inst Phys SB RAS, Krasnoyarsk 660036, Russia.
FRC KSC SB RAS, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Kovel, Ekaterina S.; Kicheeva, Arina G.; Vnukova, Natalia G.; Churilov, Grigory N.; Stepin, Evsei A.; Kudryasheva, Nadezhda S.; Kovel, Ekaterina; RFBRRussian Foundation for Basic Research (RFBR) [N18-29-19003]; RFBR, Krasnoyarsk Territory; Krasnoyarsk Regional Fund of Science [N20-44-243001]; Program of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Fundamental Study 2020-2025 (Russian Federation)

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19.

Toxic effects of the fungicide tebuconazole on the root system of fusarium-infected wheat plants / E. Shishatskaya [et al.] // Plant Physiol. Biochem. - 2018. - Vol. 132. - P400-407, DOI 10.1016/j.plaphy.2018.09.025 . - ISSN 0981-9428
Кл.слова (ненормированные):
Border cells -- Carbonylated proteins -- Free proline -- Fusarium -- Malondialdehyde -- Tebuconazole
Аннотация: The study investigates toxic effects of the fungicide tebuconazole (TEB) on Fusarium-infected wheat (Triticum aestivum) plants based on the morphological characteristics of root apices and changes in the integrated parameters of redox homeostasis, including the contents of free proline and products of peroxidation of proteins (carbonylated proteins, CP) and lipids (malondialdehyde, MDA) in roots. In two-day-old wheat sprouts infected by Fusarium graminearum, the levels of proline, CP, and border cells of root apices are higher than in roots of uninfected sprouts by a factor of 1.4, 8.0, and 3, respectively. The triazole fungicide tebuconazole (TEB) at the concentrations of 0.01, 0.10, and 1.00 ?g ml?1 of medium causes a dose-dependent decrease in the number of border cells. The study of the effects of TEB and fusarium infection on wheat plants in a 30-day experiment shows that the effect of the fungicide TEB on redox homeostasis in wheat roots varies depending on the plant growth stage and is significantly different in ecosystems with soil and plants infected by Fusarium phytopathogens. The study of the morphology of root apices shows that the toxic effects of TEB and fusarium infection are manifested in the destructive changes in root apices and the degradation of the root tip mantle. © 2018 Elsevier Masson SAS

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Держатели документа:
Siberian Federal University, 79 Svobodnyi Ave., Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
International and Interuniversity Centre for Nano Science and Nano Technology, Kottayam, Kerala, India

Доп.точки доступа:
Shishatskaya, E.; Menzyanova, N.; Zhila, N.; Prudnikova, S.; Volova, T.; Thomas, S.

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20.

Towards biological quantity theory for nominal property metrology in polyenzymatic devices with living cells / P. I. Belobrov, A. A. Evstrapov, E. N. Esimbekova [et al.] // Journal of Physics: Conference Series : Institute of Physics Publishing, 2019. - Vol. 1379: Joint IMEKO TC1-TC7-TC13-TC18 Symposium 2019 (2 July 2019 through 5 July 2019, ) Conference code: 156337, Is. 1. - Ст. 012036, DOI 10.1088/1742-6596/1379/1/012036
Кл.слова (ненормированные):
Fluidic devices -- Industrial waste disposal -- Biological measurement -- Bioluminescent systems -- Continuous measurements -- Droplet-based microfluidics -- Industrial enterprise -- Microfluidic platforms -- Numerical variables -- Silicon photomultiplier -- Microfluidics
Аннотация: Here we discuss the concepts of "biological quantity" and "nominal property" within the framework of the problem of biological measurements based on new specific results of biological analysis using a microfluidic platform and chips developed by our team earlier. It was shown that based on different microfluidic platforms it is possible to develop chips with a polyenzymatic bioluminescent system NAD(P)H:FMN-oxidoreductase-luciferase (Red + Luc), which can be used in various areas of biological analysis. Thus, disposable microfluidic chips with Red + Luc system suitable for field and indoor use were developed using continuous flow microfluidic platform. The use of droplet-based microfluidic platform allowed to develop microfluidic chips with Red + Luc system for long-term continuous measurements of water samples, for example, in places of waste disposal by industrial enterprises. The reference for comparing different biological quantities with each other in the proposed system was a photodetector, which converted non-numeric values and nominal properties recorded by a biological module Red + Luc into numerical variables. Such a reference was implemented as a portable luminometer based on silicon photomultiplier. The results allow to perform other biological measurements and to start the discussion of modern biological concepts in the language of biological measures. © 2019 IOP Publishing Ltd. All rights reserved.

Scopus
Держатели документа:
Laboratory of Bioluminescent Biotechnology, Siberian Federal University, Krasnoyarsk, 660041, Russian Federation
Institute for Analytical Instrumentation RAS, St.-Petersburg, 190103, Russian Federation
Laboratory of Photobiology, Institute of Biophysics SB RAS, Krasnoyarsk, 660036, Russian Federation
Laboratory for Digital Controlled Drugs and Theranostics, Federal Research Center krasnoyarsk Science Center SB RAS, Krasnoyarsk, 660036, Russian Federation
Research Institute of Molecular Medicine and Pathobiochemistry, Krasnoyarsk State Medical University Named after Prof. V.F. Voino-Yasenetsky, Krasnoyarsk, 660022, Russian Federation

Доп.точки доступа:
Belobrov, P. I.; Evstrapov, A. A.; Esimbekova, E. N.; Denisov, I. A.; Lukyanenko, K. A.; Osipova, E. D.; Yakimov, A. S.

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