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2-ENZYME NADH-FMN-OXIDOREDUCTASE-LUCIFERASE SYSTEM FROM LUMINESCENT BACTERIA [Text] / V. N. PETUSHKOV [et al.] // Biochem.-Moscow. - 1984. - Vol. 49, Is. 4. - P593-603. - Cited References: 24 . - 11. - ISSN 0006-2979

РУБ Biochemistry & Molecular Biology

Держатели документа:
LV KIRENSKII PHYS INST,KRASNOYARSK,USSR : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
PETUSHKOV, V.N.; KRATASYUK, G.A.; RODIONOVA, N.S.; FISH, A.M.; BELOBROV, P.I.

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A biological luciferase test for the bioluminescent assay of wheat grain infection with Fusarium [Text] / V. A. Kratasyuk [et al.] // Appl. Biochem. Microbiol. - 1998. - Vol. 34, Is. 6. - P. 622-624. - Cited References: 7 . - ISSN 0003-6838

РУБ Biotechnology & Applied Microbiology + Microbiology

Аннотация: The extent of inhibition of the bioluminescence reaction by wheat grain extracts was studied as a function of the scabby kernel content in wheat. The NADH : flavine mononucleotide oxidoreductase-luciferase bienzyme bioluminescence system was found to be the most sensitive to mycotoxins produced by fungi of the genus Fusarium. A biological luciferase test was developed for monitoring wheat grain infection with Fusarium.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
All Russia Res Inst Grain & Grain Prod, Moscow 127434, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kratasyuk, V.A.; Egorova, O.I.; Esimbekova, E.N.; Kudryashova, N.S.; Orlova, N.Y.; L'vova, L.S.

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A biological method of including mineralized human liquid and solid wastes into the mass exchange of bio-technical life support systems / S. A. Ushakova [et al.] // Advances in Space Research. - 2012. - Vol. 50, Is. 7. - P932-940, DOI 10.1016/j.asr.2012.05.023 . - ISSN 0273-1177
Кл.слова (ненормированные):
Bio-technical life support system -- Conveyor-grown uneven-aged communities of wheat and Salicornia -- Utilization of human wastes -- Age groups -- Biological methods -- Dry weight -- Expanded clay -- Harvest index -- Human waste -- Leafy vegetables -- Liquid wastes -- Mass exchange -- Mineral element -- Nutrient solution -- Plant communities -- Salicornia europaea -- Salt content -- Time interval -- Uneven-aged -- Water culture -- Wheat biomass -- Conveyors -- Incineration -- Irrigation -- Minerals -- Nutrients -- Plants (botany) -- Productivity -- Sodium chloride -- Waste incineration -- Waste utilization -- Liquids
Аннотация: The main obstacle to using mineralized human solid and liquid wastes as a source of mineral elements for plants cultivated in bio-technical life support systems (BLSS) is that they contain NaCl. The purpose of this study is to determine whether mineralized human wastes can be used to prepare the nutrient solution for long-duration conveyor cultivation of uneven-aged wheat and Salicornia europaea L. plant community. Human solid and liquid wastes were mineralized by the method of "wet incineration" developed by Yu. Kudenko. They served as a basis for preparing the solutions that were used for conveyor-type cultivation of wheat community represented by 5 age groups, planted with a time interval of 14 days. Wheat was cultivated hydroponically on expanded clay particles. To reduce salt content of the nutrient solution, every two weeks, after wheat was harvested, 12 L of solution was removed from the wheat irrigation tank and used for Salicornia europaea cultivation in water culture in a conveyor mode. The Salicornia community was represented by 2 age groups, planted with a time interval of 14 days. As some portion of the nutrient solution used for wheat cultivation was regularly removed, sodium concentration in the wheat irrigation solution did not exceed 400 mg/L, and mineral elements contained in the removed portion were used for Salicornia cultivation. The experiment lasted 4 months. The total wheat biomass productivity averaged 30.1 gВ·m -2В·day -1, and the harvest index amounted to 36.8%. The average productivity of Salicornia edible biomass on a dry weight basis was 39.3 gВ·m -2В·day -1, and its aboveground mass contained at least 20% of NaCl. Thus, the proposed technology of cultivation of wheat and halophyte plant community enables using mineralized human wastes as a basis for preparing nutrient solutions and including NaCl in the mass exchange of the BLSS; moreover, humans are supplied with additional amounts of leafy vegetables. В© 2012 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Siberian State Technological University, 82, Mir Avenue, Krasnoyarsk 660049, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Ushakova, S.A.; Tikhomirov, A.A.; Tikhomirova, N.A.; Kudenko, Y.A.; Litovka, Y.A.; Anishchenko, O.V.

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A closed ecological system as a means of providing high quality of life in an antarctic station and as a model of a life-support system for the martian mission / J. I. Gitelson [et al.] // International Astronautical Federation - 55th International Astronautical Congress 2004. - 2004. - Vol. 3: International Astronautical Federation - 55th International Astronautical Congress 2004 (4 October 2004 through 8 October 2004, Vancouver) Conference code: 69653. - P1428-1435
Кл.слова (ненормированные):
Biotechnology -- Environmental impact -- Martian surface analysis -- Photosynthesis -- Psychophysiology -- Water pollution -- Antarctic station -- Closing technology -- Ecological life support system -- Environmental pollution -- Ecosystems
Аннотация: This closing technology developed for experimental BIOS-3 facility seems to be highly expedient for providing normal life conditions in high-latitude settlements and first of all for life-support of Antarctic stations. Obvious advantages of ecological life-support system based on photosynthesis, in comparison with used now in Antarctic Continent, are: 1) highly effective cascade usage of energy for performance of all functions of life-support; 2) minimization of environmental pollution due to processing human excretions and polluted water; 3) providing crew with fresh vegetable food all-the-year-round; 4) contact with alive plants is powerful source of positive emotions and effective means of psychological relaxation; 5) significant reduction of the transport charges on delivery of foodstuff to Antarctic Continent. First of all, the usage of closed ecosystem can be recommended for high-mountainous station "Concordia". Similar ecosystems can be used in northern high-latitude settlements - Russian North, Canadian Northern territories, Alaska. Finally, the experience of closed ecological life-support system operation in Antarctic Continent can serve as the best range for modeling some aspects of Martian mission - its ecological, biotechnological and psychophysiological problems - since there are no natural conditions on the Earth closer to Mars with respect to severity of a place.

Scopus
Держатели документа:
Institute of Biophysics, SB, RAS, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, J.I.; Bartsev, S.I.; Okhonin, V.A.; Mezhevikin, V.V.

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A hypothesis about double surging climate change in the 20th century [Text] / P. V. Belolipetskii, S. I. Bartsev, A. G. Degermendzhi // Dokl. Earth Sci. - 2015. - Vol. 460, Is. 1. - P46-49, DOI 10.1134/S1028334X1501002X. - Cited References:15. - This work was supported by the Program of presidium of Russian Academyof Science "Research Scientific Studies for Development of Arctic Zoneof the Russian Federation," project no. 12. . - ISSN 1028-334X. - ISSN 1531-8354

РУБ Geosciences, Multidisciplinary
Рубрики:
REGIME SHIFTS
   NORTH PACIFIC
   OCEAN
   SYSTEM
   HEAT

WOS,
Scopus
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Computat Modeling, Krasnoyarsk, Russia.
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia.
ИВМ СО РАН
ИБФ СО РАН

Доп.точки доступа:
Belolipetskii, P.V.; Bartsev, S.I.; Degermendzhi, A.G.; presidium of Russian Academy of Science "Research Scientific Studies forDevelopment of Arctic Zone of the Russian Federation" [12]

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A low-cost underwater particle tracking velocimetry system for measuring in situ particle flux and sedimentation rate in low-turbulence environments / S. Simoncelli [et al.] // Limnol. Oceanogr. Methods. - 2019, DOI 10.1002/lom3.10341 . - Article in press. - ISSN 1541-5856
Аннотация: We describe a low-cost three-dimensional underwater particle tracking velocimetry system to directly measure particle settling rate and flux in low-turbulence aquatic environments. The system consists of two waterproof cameras that acquire stereoscopic videos of sinking particles at 48 frames s?1 over a tunable sampling volume of about 45 ? 25 ? 24 cm. A dedicated software package has been developed to allow evaluation of particle velocities, concentration and flux, but also of morphometric parameters such as particle area, sinking angle, shape irregularity, and density. Our method offers several advantages over traditional approaches, like sediment trap or expensive in situ camera systems: (1) it does not require beforehand particle collection and handling; (2) it is not subjected to sediment trap biases from turbulence, horizontal advection, or presence of swimmers, that may alter particulate load and flux; (3) the camera system enables faster data processing and flux computation at higher spatial resolution; (4) apart from the particle settling rates, the particle size distribution, and morphology is determined. We tested the camera system in Lake Stechlin (Germany) in low turbulence and mean flow, and analyzed the morphological properties and settling rates of particles to determine their sinking behavior. The particle flux assessed from conventional sediment trap measurements agreed well with that determined by our system. By this, the low-cost approach demonstrated its reliability in low turbulence environments and a strong potential to provide new insights into particulate carbon transport in aquatic systems. Extension of the method to more turbulent and advective conditions is also discussed. © 2019 The Authors. Limnology and Oceanography: Methods published by Wiley Periodicals, Inc. on behalf of Association for the Sciences of Limnology and Oceanography.

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Держатели документа:
Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB), Berlin, Germany
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Potsdam University, Institute for Biochemistry and Biology, Potsdam, Germany

Доп.точки доступа:
Simoncelli, S.; Kirillin, G.; Tolomeev, A. P.; Grossart, H. -P.

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A mathematical model of the interaction of the components in a system plant-rhizospheric microorganisms at a higher CO2 level in the atmosphere [Текст] / T. I. Pisman, L. A. Somova, N. S. Pechurkin // Biofizika. - 2002. - Vol. 47, Is. 5. - P. 920-925. - Cited References: 13 . - ISSN 0006-3029

РУБ Biophysics
Рубрики:
CARBON-DIOXIDE
ENRICHMENT
ECOSYSTEMS
Кл.слова (ненормированные):
plant -- rhizospheric microorganisms -- ecosystem -- CO2-enrichnient
Аннотация: A mathematical model describing the interaction of plants and rhizospheric microorganisms on complete mineral medium at a higher CO2 level in the atmosphere was constructed. The positive effect of CO2-enrichment on the system plant - rhizospheric micro organisms was shown. The effect of rhizospheric microorganisms on plant growth at normal and high level of carbon dioxide was demonstrated. It was shown that the biomass of plant in the system is smaller than the biomass of plant growing without microorganisms. It was experimentally demonstrated that a simple ecosystem wheat - Pseudomonas putida - artificial soil develops and functions differently than its individual constituents in the case of a wheat-artificial soil system. With unlimited nutrition and a higher CO2 level (0.06%), plants with roots inoculated with micro organisms have a smaller biomass than plants that were not inoculated with micro organisms.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pisman, T.I.; Somova, L.A.; Pechurkin, N.S.

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A Mathematical Model of the Interaction of the Components in a System Plant-Rhizospheric Microorganisms at a Higher CO2 Level in the Atmosphere / T. I. Pisman, L. A. Somova, N. S. Pechurkin // Biofizika. - 2002. - Vol. 47, Is. 5. - С. 924-925 . - ISSN 0006-3029
Кл.слова (ненормированные):
CO2-enrichment -- Ecosystem -- Plant -- Rhizospheric microorganisms
Аннотация: A mathematical model describing the interaction of plants and rhizospheric microorganisms on complete mineral medium at a higher CO2 level in the atmosphere was constructed. The positive effect of CO2-enrichment on the system plant - rhizospheric microorganisms was shown. The effect of rhizospheric microorganisms on plant growth at normal and high level of carbon dioxide was demonstrated. It was shown that the biomass of plant in the system is smaller than the biomass of plant growing without microorganisms. It was experimentally demonstrated that a simple ecosystem wheat - Pseudomonas putida - artificial soil develops and functions differently than its individual constituents in the case of a wheat-artificial soil system. With unlimited nutrition and a higher CO2 level (0.06%), plants with roots inoculated with microorganisms have a smaller biomass than plants that were not inoculated with microorganisms.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pisman, T.I.; Somova, L.A.; Pechurkin, N.S.

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A mathematical model of the interactions in the mixed culture of invertebrates and algae in the "producer-consumer" aquatic biotic cycle [Text] / T. I. Pisman, O. N. Bogdanova // Aquat. Ecol. - 2004. - Vol. 38, Is. 3. - P. 415-423. - Cited References: 19 . - ISSN 1386-2588

РУБ Ecology + Limnology + Marine & Freshwater Biology
Рубрики:
RESOURCE COMPETITION
Кл.слова (ненормированные):
competition -- metabolite interaction -- principle of competitive exclusion
Аннотация: This paper presents a mathematical model of interactions between two herbivorous invertebrates (ciliate Paramecium caudatum and rotifer Brachionus plicatilis) and two planktonic algae (Chlorella vulgaris and Scenedesmus quadricauda) spatially segregated in two compartments of a chemostat - type experimental microcosm system. The model mimics a "producer-consumer" aquatic biotic cycle, describing the dynamics of the mixed culture of ciliates and rotifers, as "consumer" compartment, feeding on the mixed algal culture, as "producer" compartment, under N-limiting conditions. We experimentally found that metabolites of the alga Scenedesmus produce an adverse effect on the reproduction of ciliate Paramecium. Taking this effect into account improved the behavior of the model, the results of which came into qualitative agreement with the experimental results. Both our experimental and modeling approaches demonstrated that, even in conditions of a spatially - segregated "producer-consumer" biotic cycle, species coexistence is impossible either in the mixed algal culture or in the mixed invertebrate culture. Scenedesmus excluded Chlorella, whereas Brachionus excluded Paramecium.

WOS
Держатели документа:
Inst Biophys SB RAS, Krasnoyarsk 660036, Russia
Krasnoyarsk State Univ, Krasnoyarsk 660041, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pisman, T.I.; Bogdanova, O.N.

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10.

A new composite material based on alumina nanofibers and detonation nanodiamonds: synthesis, characterization, and sensing application / N. O. Ronzhin, E. D. Posokhina, E. V. Mikhlina [et al.] // J. Nanopart. Res. - 2021. - Vol. 23, Is. 9. - Ст. 199, DOI 10.1007/s11051-021-05309-y. - Cited References:57. - This work is partially supported by the Russian Foundation for Basic Research, Project 18-29-19078 (E. V. Mikhlina, M. M. Simunin, I. Ryzhkov). . - ISSN 1388-0764. - ISSN 1572-896X

РУБ Chemistry, Multidisciplinary + Nanoscience & Nanotechnology + Materials
Рубрики:
ELECTROCHEMICAL ENERGY-STORAGE
SELECTIVE DETECTION
PHENOL DETECTION
Кл.слова (ненормированные):
Nanodiamonds -- Alumina nanofibers -- Composite -- Indicator system -- Phenol
Аннотация: The development of inexpensive, easy-to-produce, and easy-to-use analytical tools for detection of harmful and toxic substances is a relevant research problem with direct applications in environmental monitoring and protection. In this work, we propose a novel composite material based on alumina nanofibers and detonation nanodiamonds for detection of phenol in aqueous medium. The composite material was obtained by mixing an aqueous suspension of alumina nanofibers with a diameter of 10-15 nm and a length of several microns and a hydrosol of nanodiamonds with an average cluster size of 70 nm. The mechanisms underlying the interaction of these nanomaterials are clarified and the physicochemical properties of the composite are investigated. The SEM and TEM studies show that the obtained composite has a network structure, in which clusters of nanodiamonds (10-20 nm in diameter) are distributed over the surface of nanofibers. Coupling of nanomaterials occurs due to opposite signs of their zeta potentials, which results in electrostatic attraction and subsequent chemical bonding as indicated by the X-ray photoelectron spectroscopy and simultaneous thermal analysis. The bonding apparently occurs between functional groups (mainly carboxyl) on the surface of nanodiamonds and amphoteric hydroxyl groups on the surface of alumina nanofibers. The proposed composite allows an easy-to-perform colorimetric analysis for qualitative and quantitative determination of phenol in aqueous samples with linear response over a wide range of concentrations (0.5-106 mu M). Multiple tests have shown that the composite is reusable and retains its catalytic function for at least 1 year during storage at room temperature.

WOS
Держатели документа:
Inst Biophys SB RAS, Akademgorodok 50-50, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Svobodny 79, Krasnoyarsk 660041, Russia.
Inst Computat Modelling SB RAS, Akademgorodok 50-44, Krasnoyarsk 660036, Russia.
Inst Chem & Chem Technol SB RAS, Akademgorodok 50-24, Krasnoyarsk 660036, Russia.
Fed Res Ctr KSC SB RAS, Akademgorodok 50-38, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Ronzhin, Nikita O.; Posokhina, Ekaterina D.; Mikhlina, Elena, V; Mikhlin, Yuri L.; Simunin, Mikhail M.; Tarasova, Lyudmila S.; Vorobyev, Sergey A.; Bondar, Vladimir S.; Ryzhkov, Ilya I.; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [18-29-19078]

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11.

A noninvasive and qualitative bioluminescent assay for express diagnostics of athletes' responses to physical exertion / V. A. Kratasyuk, L. V. Stepanova, R. Ranjan [et al.] // Luminescence. - 2020, DOI 10.1002/bio.3954. - Cited References:33. - The Ministry of Science and Higher Education of the Russian Federation, Grant/Award Number: FSRZ-2020-0006; Krasnoyarsk Regional Foundation of Science, Grant/Award Number: KF-537 . - Article in press. - ISSN 1522-7235. - ISSN 1522-7243

РУБ Chemistry, Analytical
Рубрики:
SALIVARY BIOMARKERS
EXERCISE
Кл.слова (ненормированные):
athletes -- BLuc‐ -- Red coupled enzyme system -- catalase activity -- saliva -- training load
Аннотация: Upcoming professional sports authorities seek rapid noninvasive biosensing tools for regular monitoring of athletes' physiological states. The analysis of saliva through luminescence-based biosensors has been perceived as a suitable candidate for such purposes. The present study reports a qualitative bioluminescence assay based on a coupled enzyme system that consists of bacterial luciferase (BLuc) and nicotinamide adenine dinucleotide (NADH):flavin mononucleotide (FMN) oxidoreductase (Red), BLuc-Red, for the express diagnostics of athletes' stress levels before and after physical exertion. The volunteers who participated in the study were grouped as freestyle wrestlers and students who adapted to different levels of physical activities. Under physical exertion modelling conditions, the influence of participant saliva on BLuc-Red catalyzed light emission was investigated. Results showed a significant increase in residual luminescence (I-exp, mean maximum bioluminescence intensity of the experimental measurement (I-exp); I-c, luminescence intensity in control; I-exp/I-c, %) values for participants in the wrestler group while a decrease in the student group (P < 0.05). Such contrasting residual luminescence values in both groups were found to be dependent on the catalase activity of saliva. The proposed bioluminescence assay can be utilized as a potential nonspecific biosensing tool for determining the physical state of athletes under high loads.

WOS
Держатели документа:
Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Dept Biophys, Svobodny Prospect 79, Krasnoyarsk 660041, Russia.
Inst Biophys SB RAS, Fed Res Ctr Krasnoyarsk Sci Ctr SB RAS, Akademgorodok 50-50, Akademgorodok, Russia.
Krasnoyarsk State Med Univ, Minist Hlth Russian Federat, Av Partizan Zheleznyak 1, Krasnoyarsk, Russia.
Krasnoyarsk Matern & Childhood Protect Ctr, Kirenskogo St 2a, Krasnoyarsk, Russia.
Siberian Fed Univ, Sch Nonferrous Met & Mat Sci, Svobodny Prospect 79, Krasnoyarsk, Russia.
Sci Res Inst Med Problems North, Av Partizan Zheleznyak 3g, Krasnoyarsk, Russia.

Доп.точки доступа:
Kratasyuk, Valentina A.; Stepanova, Lyudmila, V; Ranjan, Rajeev; Sutormin, Oleg S.; Pande, Shubhra; Zhukova, Galina, V; Miller, Olga M.; Maznyak, Natalya, V; Kolenchukova, Oksana A.; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]; Krasnoyarsk Regional Foundation of Science [KF-537]

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12.

A novel ATP-dependent bioluminescent system from the Siberian earthworm Fridericia heliota: structure elucidation of luciferin and its analogs [Text] / V. . Petushkov [et al.] // Luminescence. - 2014. - Vol. 29. - P54-55. - Cited References: 3 . - ISSN 1522-7235. - ISSN 1522-7243

WOS
Держатели документа:
[Dubinnyi, Maxim
Tsarkova, Aleksandra
Baranov, Mikhail
Yampolsky, Ilia] Russian Acad Sci, Inst Bioorgan Chem, Moscow, Russia
[Petushkov, Valentin
Rodionova, Natalja
Shimomura, Osamu] Russian Acad Sci, Inst Biophys, Lab Photobiol, Siberian Branch, Krasnoyarsk, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V...; Dubinnyi, M...; Tsarkova, A...; Rodionova, N...; Baranov, M...; Shimomura, O...; Yampolsky, I...

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13.

A Novel Type of Luciferin from the Siberian Luminous Earthworm Fridericia heliota: Structure Elucidation by Spectral Studies and Total Synthesis [Text] / V. N. Petushkov [et al.] // Angew. Chem.-Int. Edit. - 2014. - Vol. 53, Is. 22. - P5566-5568, DOI 10.1002/anie.201400529. - Cited References: 13. - We thank Dr. Alexander O. Chizhov for recording mass spectra and Dr. K. S. Mineev for NMR analysis of synthetic intermediates. We acknowledge support from the Program of the Government of the Russian Federation "Measures to attract leading scientists to Russian educational institutions" (grant no. 11. G34.31.0058), the programs MCB RAS, President of the Russian Federation "Leading science school" (grant 3951.2012.4) and the Russian Foundation for Basic Research (grant 14-03-01015). B. M. S. was supported by a stipend from the Program of the President of the Russian Federation. . - ISSN 1433-7851. - ISSN 1521-3773

РУБ Chemistry, Multidisciplinary
Рубрики:
BIOLUMINESCENT EARTHWORM
Кл.слова (ненормированные):
bioluminescence -- luciferin -- natural products -- NMR spectroscopy -- total synthesis
Аннотация: The structure elucidation and synthesis of the luciferin from the recently discovered luminous earthworm Fridericia heliota is reported. This luciferin is a key component of a novel ATP-dependent bioluminescence system. UV, fluorescence, NMR, and HRMS spectroscopy studies were performed on 0.005 mg of the isolated substance and revealed four isomeric structures that conform to spectral data. These isomers were chemically synthesized and one of them was found to produce light when reacted with a protein extract from F. heliota. The novel luciferin was found to have an unusual extensively modified peptidic nature, thus implying an unprecedented mechanism of action.

WOS
Держатели документа:
[Petushkov, Valentin N.
Rodionova, Natalja S.
Shimomura, Osamu] Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Lab Bioluminescent Biotechnol, Krasnoyarsk 660041, Russia
[Petushkov, Valentin N.
Rodionova, Natalja S.] Russian Acad Sci, Siberian Branch, Inst Biophys, Lab Photobiol, Krasnoyarsk 660036, Russia
[Dubinnyi, Maxim A.
Tsarkova, Aleksandra S.
Baranov, Mikhail S.
Kublitski, Vadim S.
Yampolsky, Ilia V.] Russian Acad Sci, Inst Bioorgan Chem, Moscow 117997, Russia
[Shimomura, Osamu] Marine Biol Lab, Woods Hole, MA 02543 USA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V.N.; Dubinnyi, M.A.; Tsarkova, A.S.; Rodionova, N.S.; Baranov, M.S.; Kublitski, V.S.; Shimomura, O...; Yampolsky, I.V.; Government of the Russian Federation "Measures to attract leading scientists to Russian educational institutions" [11. G34.31.0058]; programs MCB RAS; Russian Federation "Leading science school" [3951.2012.4]; Russian Foundation for Basic Research [14-03-01015]; Russian Federation

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14.

A small closed ecosystem with an estimated portion of human metabolism / A. A. Tikhomirov [et al.] // Life Sci. Space Res. - 2018. - Vol. 19. - P63-67, DOI 10.1016/j.lssr.2018.10.001. - Cited References:11. - The study was supported by the Russian Science Foundation (Project No. 14-14-00599.) and carried out in the IBP SB RAS at FRC KRC SB RAS. . - ISSN 2214-5524. - ISSN 2214-5532

РУБ Astronomy & Astrophysics + Biology + Multidisciplinary Sciences

Кл.слова (ненормированные):
Closed ecosystem -- Physicochemical waste processing -- Biochemical waste -- processing -- Cycling process
Аннотация: The study describes a small closed ecosystem used to test technologies to be further employed in full-scale manned closed ecosystems. The experimental ecosystem is designed to use a certain portion of human metabolism, which is included in the gas, water, and organic waste loops of the system. In this experimental ecosystem, gas and water loops are fully closed, and the model enables processing of human waste and plant inedible biomass. A physicochemical method is used to remove pollutants from the air in the system. A human takes part in the gas exchange of the system through its respiration loop. This experimental ecosystem can be used for testing and improving new technologies to be further used in the future space stations.

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Держатели документа:
Krasnoyarsk Sci Ctr SB RAS, Inst Biophys SB RAS, Fed Res Ctr, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Tikhomirov, Alexander A.; Ushakova, Sofya A.; Velichko, Vladimir V.; Trifonov, Sergey, V; Tikhomirova, Natalia A.; Kalacheva, Galina S.; Russian Science Foundation [14-14-00599]

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15.

A study of neonothopanus nambi luminescent system / V. S. Bondar [et al.] // Luminescence. - 2012. - Vol. 27, Is. 2. - P101-102. - Cited References: 6 . - ISSN 1522-7235

РУБ Biochemistry & Molecular Biology

Держатели документа:
SB RAS, Inst Biophys, Krasnoyarsk 660036, Russia
Siberian Fed Univ, Krasnoyarsk 660041, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
Bondar, V.S.; Puzyr, A.P.; Purtov, K.V.; Medvedeva, S.E.; Rodicheva, E.K.; Kalacheva, G.S.; Gitelson, J.I.

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16.

A study of the stationary and the anomalous in the ocean surface chlorophyll distribution by satellite data [Text] / A. . Shevyrnogov, G. . Vysotskaya, E. . Shevyrnogov // Int. J. Remote Sens. - 2004. - Vol. 25: Oceans from Space Venice 2000 Symposium (OCT 09-13, 2000, Venice, ITALY), Is. 07.08.2013. - P. 1383-1387, DOI 10.1080/01431160310001592337. - Cited References: 8 . - ISSN 0143-1161

РУБ Remote Sensing + Imaging Science & Photographic Technology

Аннотация: Variability of chlorophyll concentration in the ocean is one of the most important components of the primary production process on the planet. To preserve the biosphere and to make appropriate use of it, it is imperative to have a deep insight into the long-term dynamics of the primary production on the planet. To investigate the dynamics of chlorophyll concentration based on satellite data, the Institute of Biophysics (Russian Academy of Sciences, Siberian Branch) has developed a geoinformation system. It was used to detect the areas in the global ocean that are quasistationary in relation to seasonal dynamics of chlorophyll concentration. Areas such as these, found in the Indian Ocean, are described in this work. These areas form the basis for the analysis of long-term dynamics of chlorophyll concentration. In these quasistationary zones systematic monitoring of phytopigment concentration is conducted by space-borne and marine craft. The work presents long-term satellite-based data on the space distribution of anomalous deviations of chlorophyll concentration in the ocean. An anomaly criterion is proposed and maps of Pacific Ocean areas with a high probability of anomalies are presented.

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Держатели документа:
RAS, SB, Inst Biophys, Krasnoyarsk 660036, Russia
RAS, SB, Inst Computat Modeling, Krasnoyarsk, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shevyrnogov, A...; Vysotskaya, G...; Shevyrnogov, E...

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17.

A study of the stationary and the anomalous in the ocean surface chlorophyll distribution by satellite data / A. Shevyrnogov, G. Vysotskaya, E. Shevyrnogov // International Journal of Remote Sensing. - 2004. - Vol. 25, Is. 7-8. - P1383-1387 . - ISSN 0143-1161
Кл.слова (ненормированные):
Chlorophyll -- Data reduction -- Oceanography -- Planets -- Probability -- Satellite communication systems -- Biospheres -- Remote sensing -- chlorophyll -- concentration (composition) -- remote sensing -- satellite data -- sea surface
Аннотация: Variability of chlorophyll concentration in the ocean is one of the most important components of the primary production process on the planet. To preserve the biosphere and to make appropriate use of it, it is imperative to have a deep insight into the long-term dynamics of the primary production on the planet. To investigate the dynamics of chlorophyll concentration based on satellite data, the Institute of Biophysics (Russian Academy of Sciences, Siberian Branch) has developed a geoinformation system. It was used to detect the areas in the global ocean that are quasistationary in relation to seasonal dynamics of chlorophyll concentration. Areas such as these, found in the Indian Ocean, are described in this work. These areas form the basis for the analysis of long-term dynamics of chlorophyll concentration. In these quasistationary zones systematic monitoring of phytopigment concentration is conducted by space-borne and marine craft. The work presents long-term satellite-based data on the space distribution of anomalous deviations of chlorophyll concentration in the ocean. An anomaly criterion is proposed and maps of Pacific Ocean areas with a high probability of anomalies are presented. В© 2004 Taylor and Francis Ltd.

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Держатели документа:
Institute of Biophysics SB RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Inst. Computational Modeling SB RAS, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shevyrnogov, A.; Vysotskaya, G.; Shevyrnogov, E.

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18.

A test system for tick-borne encephalitis virus detection based on bioluminescent immunoassay / A. N. Kudryavtsev, L. P. Burakova, K. A. Barinova, L. A. Frank // J. Sib. Fed. Univ. - Biol. - 2020. - Vol. 13, Is. 3. - С. 310-321, DOI 10.17516/1997-1389-0296 . - ISSN 1997-1389
Кл.слова (ненормированные):
Bioluminescent microassay -- Hybrid protein 14D5a-Rm7 -- Tick-borne encephalitis virus (TBEV)
Аннотация: The tick-borne encephalitis virus (TBEV) is the causative agent of one of the most severe human neuroinfections. The infection transmitted by ixodid ticks is spread throughout the forest and forest-steppe zones of the temperate climatic belt of the Eurasian continent, including the Siberian region of the Russian Federation. Despite the availability of commercial analytical systems for the detection of TBEV, the task of developing approaches to a quick and reliable analysis that can be performed routinely, particularly in environmental studies, remains topical. A solid-phase bioluminescent immunoassay for determining the tick-borne encephalitis virus (TBEV) in ticks was developed. The assay is based on the hybrid protein consisting of a modified thermostable version of Renilla muelleri luciferase and a single-chain mini-antibody to protein E. This unique protein had been obtained and investigated by the authors earlier. The current study describes the expression of the hybrid protein in two different strains of recombinant E. coli cells. The optimal conditions for obtaining a highly purified protein were found. The bioluminescent reaction of the luciferase domain was triggered with the help of the stable natural form of the substrate, a Ca-dependent coelenterazine-binding protein, the recombinant variant of which was obtained by the authors. The conditions for production and storage of the immunoassay components (the hybrid protein, the stable form of the luciferase substrate, and activated microplates) were determined. Using the developed test system, more than 900 tick samples were analyzed for TBEV. In terms of sensitivity (89.5%) and specificity (98.9%), the proposed method is not inferior to colorimetric detection and is much simpler and faster than the latter. © Siberian Federal University. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, FRC Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Kudryavtsev, A. N.; Burakova, L. P.; Barinova, K. A.; Frank, L. A.

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19.

Accumulation and release of 99Tc by a macrophyte of the Yenisei River (Elodea canadensis) in laboratory experiments / A. Bolsunovsky, L. Bondareva // Journal of Radioanalytical and Nuclear Chemistry. - 2008. - Vol. 277, Is. 3. - P631-636, DOI 10.1007/s10967-007-7148-5 . - ISSN 0236-5731
Кл.слова (ненормированные):
technetium 99m -- aquatic flora -- article -- biomass -- controlled study -- dry weight -- liquid scintillation counting -- macrophyte -- nonhuman -- radiation absorption -- radiation detection -- radiation dose fractionation -- radiation measurement -- radioactivity -- river -- water sampling
Аннотация: The study addresses 99Tc accumulation and release by Elodea canadensis, one of the abundant species of submerged plants in the Yenisei River. 99Tc in water samples of the "Elodea - Yenisei River water" model system and in the biomass fractions was measured using a liquid scintillation analyzer. Experiments on accumulation of 99Tc by Elodea showed that 99Tc activity concentration can reach 120В±6 Bq/g dry wt, with the concentration factor for 99Tc 2700В±500 l/kg dry wt. In experiments on 99Tc release, over 504 hours about 82% of the total 99Tc activity was released into the water from the plant; most of 99Tc was released within the first 192 hours. The data obtained using sequential chemical fractionation of biomass confirmed the experimental data on 99Tc release, which suggested that most of the biomass-bound 99Tc was adsorbed on the surface of Elodea. 99Tc tightly bound to biomass (fractions of organics and mineral residue) constituted just 17% of the total 99Tc activity. В© 2008 Akademiai Kiado, Budapest.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.; Bondareva, L.

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20.

Accumulation of 242Pu by a macrophyte of the Yenisei River (Elodea canadensis) in laboratory experiments / A. Bolsunovsky [et al.] // Chemosphere. - 2009. - Vol. 75, Is. 3. - P284-288, DOI 10.1016/j.chemosphere.2008.12.036 . - ISSN 0045-6535
Кл.слова (ненормированные):
242Pu -- Accumulation -- Laboratory experiments -- Sequential extraction technique -- Submerged plant Elodea canadensis -- Yenisei River -- sup242/supPu -- Accumulation -- Laboratory experiments -- Sequential extraction technique -- Submerged plant Elodea canadensis -- Yenisei River -- Biological materials -- Biomass -- Chemical plants -- Experiments -- Positive ions -- Rivers -- Plutonium -- concentration (composition) -- experimental study -- laboratory method -- macrophyte -- plutonium isotope -- river water -- submerged vegetation -- Eurasia -- Russian Federation -- Yenisei River -- plutonium -- article -- biomass -- chemistry -- Hydrocharitaceae -- physiology -- plant -- radiation monitoring -- river -- time -- water pollutant -- Biomass -- Hydrocharitaceae -- Plant Shoots -- Plutonium -- Radiation Monitoring -- Rivers -- Time Factors -- Water Pollutants, Radioactive
Аннотация: The study addresses 242Pu accumulation by Elodea canadensis, one of the abundant species of submerged plants in the Yenisei River. 242Pu in water samples of the "Elodea - Yenisei River water" model system and in the biomass fractions was determined ?-spectrometrically, following radiochemical recovery of 242Pu using 236Pu - a chemical yield tracer. The experiments on accumulation of 242Pu by Elodea biomass showed that the activity concentration of 242Pu can reach 21 В± 2 Bq/g dry wt, with the concentration factor for 242Pu 13100 В± 2100 L/kg dry wt. Results of chemical fractionation proved that during the first few hours of the experiment 242Pu contained in Elodea was mainly concentrated in the exchangeable and the adsorbed fractions of biomass (about 100%). As Elodea biomass accumulated 242Pu, the absolute amount of 242Pu in the exchangeable and the adsorbed fractions remained almost unchanged, although the portion of 242Pu tightly bound to biomass increased. At the end of the experiment, on day 7, 242Pu tightly bound to biomass (fractions of organics and mineral residue) constituted 43-63% (in different experiments) of the total 242Pu in the biomass. В© 2008 Elsevier Ltd. All rights reserved.

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Держатели документа:
Institute of Biophysics, Siberian Branch, the Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Institute of Geology and Mineralogy, Siberian Branch, the Russian Academy of Sciences, Novosibirsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.; Bondareva, L.; Sukhorukov, F.; Melgunov, M.

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