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Coelenterazine-dependent luciferases / S. V. Markova, E. S. Vysotski // Biochemistry Moscow. - 2015. - Vol. 80, Is. 6. - P714-732, DOI 10.1134/S0006297915060073 . - ISSN 0006-2979
Кл.слова (ненормированные):
bioluminescence -- coelenterazine -- luciferase -- luciferin -- Coelenterata -- Cypridina luciferin -- Fungi -- Hexapoda -- Mollusca -- Protozoa
Аннотация: Bioluminescence is a widespread natural phenomenon. Luminous organisms are found among bacteria, fungi, protozoa, coelenterates, worms, molluscs, insects, and fish. Studies on bioluminescent systems of various organisms have revealed an interesting feature - the mechanisms underlying visible light emission are considerably different in representatives of different taxa despite the same final result of this biochemical process. Among the several substrates of bioluminescent reactions identified in marine luminous organisms, the most commonly used are imidazopyrazinone derivatives such as coelenterazine and Cypridina luciferin. Although the substrate used is the same, bioluminescent proteins that catalyze light emitting reactions in taxonomically remote luminous organisms do not show similarity either in amino acid sequences or in spatial structures. In this review, we consider luciferases of various luminous organisms that use coelenterazine or Cypridina luciferin as a substrate, as well as modifications of these proteins that improve their physicochemical and bioluminescent properties and therefore their applicability in bioluminescence imaging in vivo. © 2015 Pleiades Publishing, Ltd.

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Держатели документа:
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Markova, S.V.; Vysotski, E.S.

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Estimating In Situ Zooplankton Non-Predation Mortality in an Oligo-Mesotrophic Lake from Sediment Trap Data: Caveats and Reality Check [Text] / O. P. Dubovskaya [et al.] // PLoS One. - 2015. - Vol. 10, Is. 7. - Ст. e0131431, DOI 10.1371/journal.pone.0131431. - Cited References:60. - This work was supported by joint projects of Russian Foundation for Basic Research and Belarusian Republican Foundation for Fundamental Research (No 14-05-90005-Bel-a to O.P.D and A.P.T., and No B14R-066 to Zh.B.). K.W.T. was supported by a Humboldt Fellowship for Experienced Researchers (Germany), M.I.G. was supported by Russian Federal Tasks of Fundamental Research (project No. 51.1.1), H.P.G. and G.K. were supported by grants from the German Science Foundation (GR 1540/20-1 and KI-853/8-1). . - ISSN 1932-6203

РУБ Multidisciplinary Sciences
Рубрики:
FRESH-WATER
NONCONSUMPTIVE MORTALITY
NONPREDATORY MORTALITY
Аннотация: Background Mortality is a main driver in zooplankton population biology but it is poorly constrained in models that describe zooplankton population dynamics, food web interactions and nutrient dynamics. Mortality due to non-predation factors is often ignored even though anecdotal evidence of non-predation mass mortality of zooplankton has been reported repeatedly. One way to estimate non-predation mortality rate is to measure the removal rate of carcasses, for which sinking is the primary removal mechanism especially in quiescent shallow water bodies. Objectives and Results We used sediment traps to quantify in situ carcass sinking velocity and non-predation mortality rate on eight consecutive days in 2013 for the cladoceran Bosmina longirostris in the oligo-mesotrophic Lake Stechlin; the outcomes were compared against estimates derived from in vitro carcass sinking velocity measurements and an empirical model correcting in vitro sinking velocity for turbulence resuspension and microbial decomposition of carcasses. Our results show that the latter two approaches produced unrealistically high mortality rates of 0.58-1.04 d(-1), whereas the sediment trap approach, when used properly, yielded a mortality rate estimate of 0.015 d(-1), which is more consistent with concurrent population abundance data and comparable to physiological death rate from the literature. Ecological implications Zooplankton carcasses may be exposed to water column microbes for days before entering the benthos; therefore, non-predation mortality affects not only zooplankton population dynamics but also microbial and benthic food webs. This would be particularly important for carbon and nitrogen cycles in systems where recurring mid-summer decline of zooplankton population due to non-predation mortality is observed.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
Swansea Univ, Dept Biosci, Swansea SA2 8PP, W Glam, Wales.
Swansea Univ, Ctr Sustainable Aquat Res, Swansea SA2 8PP, W Glam, Wales.
MU, Leibniz Inst Freshwater Ecol & Inland Fisheries, Dept Ecohydrol, D-12587 Berlin, Germany.
Natl Acad Sci Belarus Bioresources, Sci & Pract Ctr, Minsk 220072, Byelarus.
Leibniz Inst Freshwater Ecol & Inland Fisheries, Dept Expt Limnol, D-16775 Neuglobsow, Germany.
Univ Potsdam, Inst Biochem & Biol, D-14469 Potsdam, Germany.

Доп.точки доступа:
Dubovskaya, Olga P.; Tang, Kam W.; Gladyshev, Michail I.; Kirillin, Georgiy; Buseva, Zhanna; Kasprzak, Peter; Tolomeev, Aleksandr P.; Grossart, Hans-Peter; Russian Foundation for Basic Research; Belarusian Republican Foundation for Fundamental Research [14-05-90005-Bel-a, B14R-066]; Humboldt Fellowship for Experienced Researchers (Germany); Russian Federal Tasks of Fundamental Research [51.1.1]; German Science Foundation [GR 1540/20-1, KI-853/8-1]

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Application of Enzyme Bioluminescence in Ecology [Text] / E. Esimbekova, V. Kratasyuk, O. Shimomura // Adv. Biochem. Eng. Biotechnol. : SPRINGER-VERLAG BERLIN, 2014. - Vol. 144. - P67-109. - (Advances in Biochemical Engineering-Biotechnology), DOI 10.1007/978-3-662-43385-0_3. - Cited References:85 . -

РУБ Biotechnology & Applied Microbiology
Рубрики:
BACTERIAL LUCIFERASE
   IN-VITRO
   PYRETHROID INSECTICIDES
   FRESH-WATER
Кл.слова (ненормированные):
Bioluminescence -- Ecological monitoring -- Enzymatic assay -- Immobilization -- Integral water toxicity -- Luciferase
Аннотация: This review examines the general principles of bioluminescent enzymatic toxicity bioassays and describes the applications of these methods and the implementation in commercial biosensors. Bioluminescent enzyme system technology (BEST) has been proposed in the bacterial coupled enzyme system, wherein NADH: FMN-oxidoreductase-luciferase substitutes for living organisms. BEST was introduced to facilitate and accelerate the development of cost-competitive enzymatic systems for use in biosensors for medical, environmental, and industrial applications. For widespread use of BEST, the multicomponent reagent "Enzymolum'' has been developed, which contains the bacterial luciferase, NADH: FMN-oxidoreductase, and their substrates, co-immobilized in starch or gelatin gel. Enzymolum is the central part of Portable Laboratory for Toxicity Detection (PLTD), which consists of a biodetector module, a sampling module, a sample preparation module, and a reagent module. PLTD instantly signals chemical-biological hazards and allows us to detect a wide range of toxic substances. Enzymolum can be integrated as a biological module into the portable biodetector-biosensor originally constructed for personal use. Based on the example of Enzymolum and the algorithm for creating new enzyme biotests with tailored characteristics, a new approach was demonstrated in biotechnological design and construction. The examples of biotechnological design of various bioluminescent methods for ecological monitoring were provided. Possible applications of enzyme bioassays are seen in the examples for medical diagnostics, assessment of the effect of physical load on sportsmen, analysis of food additives, and in practical courses for higher educational institutions and schools. The advantages of enzymatic assays are their rapidity (the period of time required does not exceed 3-5 min), high sensitivity, simplicity and safety of procedure, and possibility of automation of ecological monitoring; the required luminometer is easily available.

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Держатели документа:
Inst Biophys SB RAS, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
ИБФ СО РАН

Доп.точки доступа:
Esimbekova, Elena; Kratasyuk, Valentina; Shimomura, Osamu

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The smallest natural high-active luciferase: Cloning and characterization of novel 16.5-kDa luciferase from copepod Metridia longa [Text] / S. V. Markova [et al.] // Biochem. Biophys. Res. Commun. - 2015. - Vol. 457, Is. 1. - P77-82, DOI 10.1016/j.bbrc.2014.12.082. - Cited References:20. - The cloning of cDNA encoding MLuc7 luciferase of M. longa was supported by Bayer AG (Germany); all other studies - by the grant 14-14-01119 of the Russian Science Foundation. We declare that authors have no conflict of interest. . - ISSN 0006-291X. - ISSN 1090-2104

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
CDNA CLONING
   SECRETED LUCIFERASE
   ESCHERICHIA-COLI
   EXPRESSION
Кл.слова (ненормированные):
Bioluminescence -- Coelenterazine -- Copepod luciferase -- Mammalian -- expression -- Real-time imaging
Аннотация: Coelenterazine-dependent copepod luciferases containing natural signal peptide for secretion are a very convenient analytical tool as they enable monitoring of intracellular events with high sensitivity, without destroying cells or tissues. This property is well suited for application in biomedical research and development of cell-based assays for high throughput screening. We report the cloning of cDNA gene encoding a novel secreted non-allelic 16.5-kDa isoform (MLuc7) of Metridia longa luciferase, which, in fact, is the smallest natural luciferase of known for today. Despite the small size, isoform contains 10 conservative Cys residues suggesting the presence of up to 5 S-S bonds. This hampers the efficient production of functionally active recombinant luciferase in bacterial expression systems. With the use of the baculovirus expression system, we produced substantial amounts of the proper folded MLuc7 luciferase with a yield of similar to 3 mg/L of a high purity protein. We demonstrate that MLuc7 produced in insect cells is highly active and extremely thermostable, and is well suited as a secreted reporter when expressed in mammalian cells ensuring higher sensitivity of detection as compared to another Metridia luciferase isoform (MLuc164) which is widely employed in real-time imaging. (C) 2014 Elsevier Inc. All rights reserved.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Photobiol Lab, Siberian Branch, Krasnoyarsk, Russia.
Siberian Fed Univ, Chair Biophys, Krasnoyarsk, Russia.
ИБФ СО РАН

Доп.точки доступа:
Markova, Svetlana V.; Larionova, Marina D.; Burakova, Ludmila P.; Vysotski, Eugene S.; Bayer AG (Germany); Russian Science Foundation [14-14-01119]

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The population dynamics of cancer incidence: The model of a second-order phase transition / V. G. Soukhovolsky [et al.] // Biophysics. - 2015. - Vol. 60, Is. 4. - P639-646, DOI 10.1134/S0006350915040235 . - ISSN 0006-3509
Кл.слова (ненормированные):
cancer incidence -- models of cancer incidence development -- phase transitions of the second order
Аннотация: This paper considers an approach to the description of the age-specific and time-dependent dynamics of cancer incidence. This approach is based on a model that describes the age-specific dynamics of cancer as a phase transition of the second order and is widely used in the study of physical systems. The proposed model of cancer development as the second-order phase transitions is quite consistent with the data of medical statistics, is well described using only two free parameters, easily verified by the statistical data, and is interpreted well. The applicability of the model of second-order phase transitions to the description of the processes in the nonphysical systems is likely to be determined by the versatile nature of the processes that occur during the phase transitions. © 2015, Pleiades Publishing, Inc.

Scopus
Держатели документа:
International Scientific Center for Research on Extreme States of an Organism, Krasnoyarsk Scientific Center, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50, Krasnoyarsk, Russian Federation
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50/50, Krasnoyarsk, Russian Federation
Hillel Yaffe Medical Center, Ha-Shalom str., Hadera, Israel
Research Institute for Complex Problems of Hygiene and Occupational Diseases, ul. Kutuzova 23, Novokuznetsk, Kemerovo oblast, Russian Federation
Voino-Yasenetsky Krasnoyarsk State Medical University, ul. Partizana Zheleznyaka 1, Krasnoyarsk, Russian Federation
Siberian Federal University, Svobodniy prosp. 79, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Soukhovolsky, V. G.; Ivanova, Y. D.; Shulman, K.; Mazharov, V. F.; Tarasova, I. V.; Tarasova, O. V.; Khlebopros, R. G.

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Bioassay of products of organic waste mineralization: An approach for closed ecosystems / S. V. Trifonov, Y. A. Kudenko, A. A. Tikhomirov // Ecol. Eng. - 2016. - Vol. 91. - P139-142, DOI 10.1016/j.ecoleng.2016.02.032 . - ISSN 0925-8574
Кл.слова (ненормированные):
Closed life support systems (CLSS) -- Organic waste -- Physicochemical oxidation -- Products of mineralization -- Radish growth -- Bioassay -- Electric fields -- Solutions -- Space flight -- Wastes -- Alternating current -- Closed life support systems (CLSS) -- Gaseous environments -- High sensitivity -- Liquid products -- Organic wastes -- Plant productivity -- Products of mineralization -- Mineralogy -- Embryophyta -- Raphanus sativus
Аннотация: The study assesses the usability of the method of organic waste mineralization in the hydrogen peroxide aqueous solution under application of an alternating current electric field in closed life support systems (CLSS). The effects of the mineralized organic wastes on the higher plant component of the CLSS intended for space flights were studied experimentally. Radish plants, representing the higher plant compartment of the CLSS, were chosen for their high sensitivity to the pollution of the gaseous environment. The study showed that plant productivity remained comparable to that of control plants in the experiments with gaseous and liquid products of mineralization of human wastes and inedible plant parts used both separately and simultaneously. Results of the study suggest that this method is eco-friendly and suitable for use in the CLSS. © 2016 Elsevier B.V.

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Смотреть статью
Держатели документа:
Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Akademgorodok, 50/50, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Trifonov, S. V.; Kudenko, Y. A.; Tikhomirov, A. A.

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Mercury, selenium and fish oils in marine food webs and implications for human health / M. O. Gribble [et al.] // J. Mar. Biol. Assoc. U.K. - 2016. - Vol. 96, Is. 1. - P43-59, DOI 10.1017/S0025315415001356 . - ISSN 0025-3154
Кл.слова (ненормированные):
Docosahexaenoic acid -- Ecotoxicology -- Eicosapentaenoic acid -- Fish oils -- Mercury -- N-3 fatty acids -- Oceans and human health -- OHH -- Public health -- Selenium
Аннотация: Humans who eat fish are exposed to mixtures of healthful nutrients and harmful contaminants that are influenced by environmental and ecological factors. Marine fisheries are composed of a multitude of species with varying life histories, and harvested in oceans, coastal waters and estuaries where environmental and ecological conditions determine fish exposure to both nutrients and contaminants. Many of these nutrients and contaminants are thought to influence similar health outcomes (i.e., neurological, cardiovascular, immunological systems). Therefore, our understanding of the risks and benefits of consuming seafood require balanced assessments of contaminants and nutrients found in fish and shellfish. In this paper, we review some of the reported benefits of fish consumption with a focus on the potential hazards of mercury exposure, and compare the environmental variability of fish oils, selenium and mercury in fish. A major scientific gap identified is that fish tissue concentrations are rarely measured for both contaminants and nutrients across a range of species and geographic regions. Interpreting the implications of seafood for human health will require a better understanding of these multiple exposures, particularly as environmental conditions in the oceans change. © Marine Biological Association of the United Kingdom, 2015.

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Держатели документа:
Department of Preventive Medicine, University of Southern California, Keck School of Medicine, Los Angeles, CA, United States
School of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, NY, United States
Department of Environmental Health Sciences, University at Albany School of Public Health, State University of New York, Rensselaer, NY, United States
Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, SC, United States
Department of Veterinary Medicine, College of Natural Science and Mathematics, University of Alaska Fairbanks, Fairbanks, AK, United States
Institute of Biophysics of Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Department of Biological Sciences, Dartmouth College, Hanover, NH, United States

Доп.точки доступа:
Gribble, M. O.; Karimi, R.; Feingold, B. J.; Nyland, J. F.; O'Hara, T. M.; Gladyshev, M. I.; Chen, C. Y.

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Prospects for using a full-scale installation for wet combustion of organic wastes in closed life support systems / S. V. Trifonov, Y. A. Kudenko, A. A. Tikhomirov // Life Sci. Space Res. - 2015. - Vol. 7. - P15-21, DOI 10.1016/j.lssr.2015.08.003 . - ISSN 2214-5524
Кл.слова (ненормированные):
Automatic control -- Human waste -- Life support system -- Physicochemical oxidation -- Radish growth -- hydrogen peroxide -- alternating current -- Article -- closed life support system -- combustion -- control system -- electric field -- electricity -- human -- microclimate -- mineralization -- organic waste -- oxidation -- radish -- recycling -- scale up -- waste -- wet combustion
Аннотация: The issue of recycling organic wastes in closed life support systems (CLSS) includes both fundamental aspects of environmental safety of the recycled products and their effective involvement in material cycles and technical aspects related to the structure of the system and the crew's demands. This study estimates the effectiveness of wet combustion of different amounts of organic wastes in hydrogen peroxide under application of an alternating current electric field. The study also addresses the possibility of controlling the process automatically. The results show that processing of greater amounts of wastes reduces specific power consumption and shortens the duration of the process, without significantly affecting the level of oxidation of the products. An automatic control system for a semi-commercial installation has been constructed and tested experimentally. The solution of mineralized human wastes prepared in the automatically controlled process in this installation was successfully used to grow radish plants, with the main production parameters being similar to those of the control. © 2015 The Committee on Space Research (COSPAR). Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, Akademgorodok 50/50, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Trifonov, S. V.; Kudenko, Y. A.; Tikhomirov, A. A.

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Applications of Luminous Bacteria Enzymes in Toxicology [Text] / V. A. Kratasyuk, E. N. Esimbekova // Comb. Chem. High Throughput Screen. - 2015. - Vol. 18, Is. 10. - P952-959, DOI 10.2174/1386207318666150917100257. - Cited References:88. - The research was supported by the Russian Science Foundation, project No. 15-19-10041. . - ISSN 1386-2073. - ISSN 1875-5402

РУБ Biochemical Research Methods + Chemistry, Applied + Pharmacology &
Рубрики:
NADHFMN-OXIDOREDUCTASE-LUCIFERASE
HUMIC SUBSTANCES
BIOLUMINESCENT
Кл.слова (ненормированные):
Bioluminescence -- bioluminescent toxicity enzymatic assay -- immobilization -- of enzymes -- luciferase -- total toxicity
Аннотация: This review describes the principle and applications of bioluminescent enzymatic toxicity bioassays. This type of assays uses bacterial coupled enzyme systems: NADH: FMN-oxidoreductase and luciferase to replace living organisms in developing cost-competitive biosensors for environmental, medical and industrial applications. These biosensors instantly signal chemical and biological hazards and allow for detecting a great amount of toxic compounds with advantages associated with fast results, high sensitivity, simplicity, low cost and safety of the procedure.

WOS
Держатели документа:
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Inst Biophys, Siberian Branch, Photobiol Lab, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Kratasyuk, Valentina A.; Esimbekova, Elena N.; Russian Science Foundation [15-19-10041]

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10.

Stoichiometric constraints and complete closure of long-term life support systems [Text] / S. I. Bartsev ; ed.: DL Henninger, AE Drysdale, E Drysdale, // SPACE LIFE SCIENCES: LIFE SUPPORT SYSTEMS AND BIOLOGICAL SYSTEMS UNDER INFLUENCE OF PHYSICAL FACTORS. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2004. - Vol. 34: 2nd World Space Congress/34th COSPAR Scientific Assembly (OCT 10-19, 2002, HOUSTON, TX), Is. 7. - P. 1509-1516, DOI 10.1016/j.asr.2003.04.069. - Cited References: 14 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Biophysics + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences

Кл.слова (ненормированные):
long-term life support systems -- stoichiometric constraints
Аннотация: High closure of matter recycling is an obvious requirement for long-term life support systems (LSS). Biological species are obligate components of the LSS since physical/chemical components are not able yet to provide food for crew. However including biological species into LSS is difficult due to specific stoichiometric configuration of their inputs and outputs. Formally the problem is to estimate the ability for given set of species to provide complete closure of LSS. Two possible models of metabolism organization can be considered: rigid and flexible ones. Stoichiometric analyses showed that the rigid metabolism case is not typical and takes place with very specific requirements. The flexible metabolic model can be applied to describing wide range of systems. Some formal indications of ability to provide complete closure and stationarity of LSS state are considered in the paper. These indications establish some constraints on the form of mathematical models intended to describe artificial and natural ecological systems. (C) 2004 COSPAR. Published by Elsevier Ltd. All rights reserved.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bartsev, S.I.; Henninger, DL \ed.\; Drysdale, AE \ed.\; Drysdale,, E \ed.\

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11.

Using DCMU-fluorescence method for the identification of dominant phytoplankton groups [Text] / N. A. Gaevsky [et al.] // J. Appl. Phycol. - 2005. - Vol. 17, Is. 6. - P. 483-494, DOI 10.1007/s10811-005-2903-x. - Cited References: 28 . - ISSN 0921-8971

РУБ Biotechnology & Applied Microbiology + Marine & Freshwater Biology
Рубрики:
IN-VIVO
   CHLOROPHYLL FLUORESCENCE
   FLUOROMETRIC METHOD
   SITU
   PHOTOSYNTHESIS
   CYANOBACTERIA
   ALGAE
   EVOLUTION
   CULTURES
   LAKES
Кл.слова (ненормированные):
algae -- chlorophyll -- fluorescence -- vizualization
Аннотация: For the identification of ecologically significant dominant groups of phytoplanktonic algae a polychromatic DCMU-induced fluorescence method is recommended. A special fluorometer equipped with a system of replaceable filters is used to differentiate three regions of the spectrum (410 +/- 20, 510 +/- 20 and 540 +/- 10 nm) that can excite the basic light-harvesting pigments. Total and differential (for every algal taxon studied) chlorophyll a calculated from the fluorescence signals is in good agreement with biomass estimates from direct cell counts for several different trophic types of aquatic systems. This is made possible by the vizualization of the ratios of fluorescence signal values in their own coordinates: first, to decide whether it is necessary to correct linear equations in order to eliminate negative solutions; second, to determine the possibility of nulling the negative solution if a point is situated close to a side of the triangle; and third, to reduce the number of linear algebraic equations to two if the points are situated along one of the triangle sides or to one if the points are gathered at the apex. The polychromatic DCMU-induced fluorescence method can be used for monitoring natural phytoplankton populations to detect changes in their taxonomic structure.

WOS
Держатели документа:
Krasnoyarsk State Univ, Dept Biol, Krasnoyarsk, Russia
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gaevsky, N.A.; Kolmakov, V.I.; Anishchenko, O.V.; Gorbaneva, T.B.

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12.

Spatial and temporal anomalies of sea surface temperature in global scale (by space-based data) [Text] / A. . Shevyrnogov, G. . Vysotskaya, E. . Shevyrnogov ; ed. P Schlussel [et al.] // CLIMATE CHANGE PROCESSES IN THE STRATOSPHERE, EARTH-ATMOSPHERE-OCEAN SYSTEMS, AND OCEANOGRAPHIC PROCESSES FROM SATELLITE DATA. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2004. - Vol. 33: 2nd World Space Congress/34th COSPAR Scientific Assembly (OCT 10-19, 2002, HOUSTON, TX), Is. 7. - P. 1179-1183, DOI 10.1016/S0273-1177(03)00369-7. - Cited References: 6 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Environmental Sciences + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences + Remote Sensing

Кл.слова (ненормированные):
temperature -- anomalies -- hydrological structure -- chlorophyll -- space-based data
Аннотация: The work presents the data on the spatial distribution of temperature anomalies in the Global Ocean, based on the long-term measurements with the AVHRR satellite equipment. It is noteworthy that such results can be only obtained by using long time series of satellite images. To obtain them by ship-borne methods is actually impossible. The proposed anomaly criterion is equal to a difference between minimum/maximum and average temperature, normed for the mean square deviation. The difference is calculated from the whole time series (with regard to a season). Based on this criterion, anomalies can be distinguished on the background of usual seasonal dynamics at various absolute values of deviations of the measured parameter. Besides, rare, single, fluctuations can be also revealed. Based on the proposed criterion, connected zones have been defined. These zones occur almost in every part of the Global Ocean. The data obtained have been analyzed in association with the CZCS (1978-1986) and SeaWiFS satellite images in order to compare them with the areas of anomalous dynamics of chlorophyll concentration and the regions quasistationary in the seasonal dynamics of chlorophyll. Anomalies in the temperature dynamics are of exceptional importance for the formation of climatic deviations; they affect the ocean-atmosphere interaction and reflect variations in the hydrological structure of the surface waters. The work presents a comparison with the dynamics of chlorophyll concentration distribution determined from the SeaWiFS data for the Atlantic Ocean. (C) 2003 COSPAR. Published by Elsevier Ltd. All rights reserved.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, SB, Krasnoyarsk 660036, Russia
Russian Acad Sci, Inst Computat Modeling, SB, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shevyrnogov, A...; Vysotskaya, G...; Shevyrnogov, E...; Schlussel, P \ed.\; Stuhlmann, R \ed.\; Campbell, JW \ed.\

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13.

Results of analysis of spatial and temporal long-term stability of quasistationary areas in the Atlantic Ocean based on multi-satellite measurements (CZCS and SeaWiFS data) [Text] / A. . Shevyrnogov, G. . Vysotskaya, E. . Shevyrnogov ; ed. P Schlussel [et al.] // CLIMATE CHANGE PROCESSES IN THE STRATOSPHERE, EARTH-ATMOSPHERE-OCEAN SYSTEMS, AND OCEANOGRAPHIC PROCESSES FROM SATELLITE DATA. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2004. - Vol. 33: 2nd World Space Congress/34th COSPAR Scientific Assembly (OCT 10-19, 2002, HOUSTON, TX), Is. 7. - P. 1184-1188, DOI 10.1016/S0273-1177(03)00366-1. - Cited References: 6 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Environmental Sciences + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences + Remote Sensing

Кл.слова (ненормированные):
chlorophyll -- seasonal dynamics -- hydrological structure -- space-based data
Аннотация: The paper analyzes the stability of areas in the Global Ocean, which are quasistationary with respect to the seasonal dynamics of chlorophyll concentration. Such areas were first discovered based on the CZCS data. They occur almost in all regions of the Global Ocean. They reflect typical seasonal dynamics of phytoplankton, which depends on both large ocean currents and local hydrological instability. It is related to the displacement of frontal zones and to local hydrological anomalies. Since the completion of the CZCS work there has been a 10 years gap in monitoring chlorophyll spatial distribution dynamics in the ocean. The reason is the long absence of equipment on the orbit, which would be suitable for the measurement of chlorophyll concentration. Recently, SeaWiFS data have been accumulated and their quantity is sufficient for the crude statistical analysis of image time series for 25 years. Thus, it has become possible to obtain data on the long-term stability of quasistationary areas and, therefore, on the stability of large-scale hydrological phenomena in the Atlantic Ocean. (C) 2003 COSPAR. Published by Elsevier Ltd. All rights reserved.

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Держатели документа:
Russian Acad Sci, Inst Biophys, SB, Krasnoyarsk 660036, Russia
Russian Acad Sci, Inst Computat Modeling, SB, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shevyrnogov, A...; Vysotskaya, G...; Shevyrnogov, E...; Schlussel, P \ed.\; Stuhlmann, R \ed.\; Campbell, JW \ed.\

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14.

Immobilization of bioluminescent systems and their applications [Text] / E. N. Esimbekova, V. A. Kratasyuk ; ed. A Tsuji [et al.] // Bioluminescence & Chemiluminescence: Progress and Perspectives : WORLD SCIENTIFIC PUBL CO PTE LTD, 2005. - 13th International Symposium on Bioluminescence and Chemiluminescence (AUG 02-06, 2004, Yokohama, JAPAN). - P. 237-240, DOI 10.1142/9789812702203_0055. - Cited References: 14 . - ISBN 981-256-118-8

РУБ Biochemical Research Methods + Biochemistry & Molecular Biology + Chemistry, Applied + Chemistry, Physical + Optics
Рубрики:
ACIDS

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Esimbekova, E.N.; Kratasyuk, V.A.; Tsuji, A \ed.\; Matsurnoto, M \ed.\; Maeda, M \ed.\; Kricka, LJ \ed.\; Kricka,, J \ed.\

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15.

CONTINUOUS CULTIVATION OF SOME WATER INVERTEBRATES / V. E. Kokova, N. I. Spitskaya // . - 1984. - Third European Congress on Biotechnology., Munich, W Ger) Conference code: 6973. - P. 539-543
Кл.слова (ненормированные):
MARINE BIOLOGY -- ARTIFICIAL ECOLOGICAL SYSTEMS -- CONTINUOUS CULTIVATION -- POPULATION GROWTH RATE -- RANGE CONDITIONS -- SUSPENDED NUTRIENTS -- WATER INVERTEBRATES -- ECOLOGY
Аннотация: Continuous cultivation of water invertebrates facilitates the study of their biological characteristics and productivity and hence contributes to creation of artifical ecological systems. When applying the technique, suspended nutrients are being passed through the cultivator and culture is outflowing continuously, with the volume inside the vessel being constant. The objective of the study is to examine the effect of a range conditions of cultivating on population growth rate.

Scopus
Держатели документа:
Acad of Sciences of the USSR, Inst, of Biophysics, Krasnoyarsk, USSR, Acad of Sciences of the USSR, Inst of Biophysics, Krasnoyarsk, USSR
ИБФ СО РАН

Доп.точки доступа:
Kokova, V.E.; Spitskaya, N.I.

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16.

Application of Enzyme Bioluminescence for Medical Diagnostics [Text] / L. A. Frank, V. V. Krasitskaya // Adv. Biochem. Eng. Biotechnol. : SPRINGER-VERLAG BERLIN, 2014. - Vol. 144. - P175-197. - (Advances in Biochemical Engineering-Biotechnology), DOI 10.1007/978-3-662-43385-0_6. - Cited References:63 . -

РУБ Biotechnology & Applied Microbiology
Рубрики:
RESONANCE ENERGY-TRANSFER
POLYMERASE-CHAIN-REACTION
LUCIFERASE
Кл.слова (ненормированные):
Bioluminescence -- Ca2+-regulated photoprotein -- Diagnostics -- Immunoassay -- Luciferase -- Nucleic acid hybridization assay
Аннотация: Nowadays luciferases are effectively used as analytical instruments in a great variety of research fields. Of special interest are the studies dealing with elaboration of novel analytical systems for the purposes of medical diagnostics. The ever-expanding spectrum of clinically important analytes accounts for the increasing demand for new techniques for their detection. In this chapter we have made an attempt to summarize the results on applications of luciferases as reporters in binding assays including immunoassay, nucleic acid hybridization assay, and so on. The data over the last 15 years have been analyzed and clearly show that luciferase-based assays, due to extremely high sensitivity, low cost, and the lack of need for skilled personnel, hold much promise for clinical diagnostics.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Photobiol Lab, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
ИБФ СО РАН

Доп.точки доступа:
Frank, Ludmila A.; Krasitskaya, Vasilisa V.

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17.

Experimental and mathematical modeling of the consumer's influence on productivity of algae in a model aquatic ecosystem [Text] / T. I. Pisman, Y. V. Galayda, I. M. Shirobokova ; ed. YV Galayd // SPACE LIFE SCIENCES: CLOSED ECOLOGICAL SYSTEMS: EARTH AND SPACE APPLICATIONS. Ser. ADVANCES IN SPACE RESEARCH-SERIES : PERGAMON-ELSEVIER SCIENCE LTD, 2005. - Vol. 35: Workshop on Closed Ecological Systems (JUL, 2004, Paris, FRANCE), Is. 9. - P. 1521-1527, DOI 10.1016/j.asr.2004.12.048. - Cited References: 10 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Ecology + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences

Кл.слова (ненормированные):
producer -- consumer -- nitrogen cycling -- mathematical modeling
Аннотация: A "producer-consumer" (Chlorella vulgaris-Paramecium caudatum) closed aquatic system has been investigated experimentally and theoretically. It has been found that there is a direct relationship between the growth of the paramecia population and their release of ammonia nitrogen, which is the best form of nitrogen for Chlorella growth. The theoretical study of a model of a "producer-consumer" aquatic biotic cycle with spatially separated compartments has confirmed the contribution of paramecia to nitrogen cycling. It has been shown that an increase in the concentration of nitrogen released as metabolites of paramecia is accompanied by an increase in the productivity of microalgae. (c) 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.

WOS
Держатели документа:
Krasnoyarsk State Univ, Dept Biophys, Krasnoyarsk 660041, Russia
RAS, SB, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pisman, T.I.; Galayda, Y.V.; Shirobokova, I.M.; Galayd, YV \ed.\

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18.

Pre-biotic stage of life origin under non-photo synthetic conditions [Text] / S. I. Bartsev, V. V. Mezhevikin ; ed. SI Bartse // SPACE LIFE SCIENCES: CLOSED ECOLOGICAL SYSTEMS: EARTH AND SPACE APPLICATIONS. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2005. - Vol. 35: Workshop on Closed Ecological Systems (JUL, 2004, Paris, FRANCE), Is. 9. - P. 1643-1647, DOI 10.1016/j.asr.2005.04.072. - Cited References: 14 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Ecology + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences

Кл.слова (ненормированные):
life origin -- pre-biotic autocatalytic system -- phase-separated autocatalytic particles -- multivariate oligomeric autocatalyst
Аннотация: Spontaneous assembling of a simplest bacterial cell even if all necessary molecules are present in a solution seems to be extremely rare event and from the scientific standpoint has to be considered as impossible. Therefore, a predecessor of a living cell has to be very simple for providing its self-assembling and at the same time it should be able of progressive increase in complexity. Now phase-separated particles, first of all micelles, are put forward as possible predecessors of living cell. According to the offered working concept only phase-separated particles possessing autocatalytic properties can be considered as predecessors of living cells. The first stage of evolution of these phase-separated autocatalytic systems is the appearance of pre-biotic metabolism providing synthesis of amphiphiles for formation of capsules of these systems. This synthesis is maintained by the energy of a base reaction being a component of a planet-chemical cycle. Catalytic system providing functioning of pre-biotic metabolism is based on multivariate oligomeric autocatalyst, which reproduces itself from monomers, penetrating the particles from the outside. Since the autocatalyst realizes random polymerization then a collection of other oligomers possessing different catalytic functions is produced. In the paper the functioning of multivariate oligomeric autocatalyst in flow reactor is analyzed. (c) 2005 Published by Elsevier Ltd on behalf of COSPAR.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Lab Theoret Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bartsev, S.I.; Mezhevikin, V.V.; Bartse, SI \ed.\

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19.

Applications of luminous bacteria enzymes in toxicology / V. A. Kratasyuk, E. N. Esimbekova // Comb. Chem. High Throughput Screen. - 2015. - Vol. 18, Is. 10. - P952-959 . - ISSN 1386-2073
Кл.слова (ненормированные):
Bioluminescence -- Bioluminescent toxicity enzymatic assay -- Immobilization of enzymes -- Luciferase -- Total toxicity
Аннотация: This review describes the principle and applications of bioluminescent enzymatic toxicity bioassays. This type of assays uses bacterial coupled enzyme systems: NADH:FMN-oxidoreductase and luciferase to replace living organisms in developing cost-competitive biosensors for environmental, medical and industrial applications. These biosensors instantly signal chemical and biological hazards and allow for detecting a great amount of toxic compounds with advantages associated with fast results, high sensitivity, simplicity, low cost and safety of the procedure. © 2015 Bentham Science Publishers.

Scopus
Держатели документа:
Siberian Federal University, Svobodnii Ave., 79, Krasnoyarsk, Russian Federation
Photobiology Laboratory, Russian Academy of Sciences, Siberian Branch, Institute of Biophysics SB RAS, Akademgorodok 50/50, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Kratasyuk, V. A.; Esimbekova, E. N.

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20.

Development of human exometabolite deep mineralization method for closed ecosystems / A. A. Tikhomirov [et al.] // Dokl. Biochem. Biophys. - 2016. - Vol. 470, Is. 1. - P316-318, DOI 10.1134/S1607672916050021. - Cited References:9. - The study was supported by the Russian Science Foundation (project no. 14-14-00599). . - ISSN 1607-6729. - ISSN 1608-3091

РУБ Biochemistry & Molecular Biology + Biophysics

Аннотация: Methods of physicochemical further oxidation of hardly soluble sediment obtained from "wet combustion" of human exometabolites applied to space-purpose Bio Technological Life Support Systems (BTLLS) were studied. Most hardly dissoluble sediment containing Ca, P, Mg, and other essential plant nutrition elements were shown to dissolve in H2O2 and HNO3 aqueous media activated by alternating electric current. Dissolved additional mineral elements allowed (as demonstrated for lettuce) to increase the productivity of BTLLS phototrophic unit plants more than twice, which is comparable to their productivity on standard Knop solution with balanced chemical composition. Thus, dissolved mineral elements can be involved into BTLLS turnover process and increase its closure degree.

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Смотреть статью,
Scopus
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Tikhomirov, A. A.; Trifonov, S. V.; Morozov, E. A.; Kudenko, Yu. A.; Kalacheva, G. S.; Ushakova, S. A.; Russian Science Foundation [14-14-00599]

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