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Вид документа : Однотомное издание
Шифр издания : Е041/М 54
Автор(ы) : Мазин, Александр Владимирович, Кузнеделов К. Д., Краев А. С., Холодилов Н. Г.
Заглавие : Методы молекулярной генетики и генной инженерии : научное издание
Выходные данные : Новосибирск: Наука. Сиб. отд-ние, 1990
Колич.характеристики :247 с.
Коллективы : АН СССР. Сибирское отделение, Институт цитологии и генетики (Новосибирск)
Примечания : Библиогр. в конце глав. - Авт. указаны на обороте тит. л.
ISBN, Цена 5-02-029565-5: 3.10, 3.10, р.
ГРНТИ : 34.15.27 + 34.15.23 + 34.23.19
УДК : 577.21.08
ББК : Е041.10в7 + Е041.15в7
Предметные рубрики: Молекулярная генетика
Генная инженерия
Аннотация: Выделение ДНК... 7 Эндонуклеазы рестрикции II типа: свойства иприменение... 14 Введение метки в ДНК... 25 Блот-гибридизация ДНК накапроновых мембранах... 35 Трансформация E. coli плазмидной ДНК... 39Коннекторный способ клонирования кДНК... 44 Получение геномныхбиблиотек в -векторах... 56 Детекция рекомбинантной ДНК методоммолекулярной гибридизации... 74 Белоксинтезирующие системы in vitro иin vivo... 80 Направленный мутагенез in vitro. Индукция транзиций GC AT... 91 Метод килосеквенирования... 99 Определение нуклеотиднойпоследовательности ДНК методом Максама-Гилберта... 107 Клонирование исеквенирование в М13... 115 Анализ функциональных сайтов в геномахпро- и эукариот... 154DNA separation... 7 Type II restriction endonuclease:properties and application... 14 DNA labelling... 25 DNA blothybridization on kapron membranes... 35 E. coli plasmid DNAtransformation... 39 Connection method of cDNA cloning... 44Producing genomic libraries in lambda-vectors... 56 Recombinant DNAdetection by molecular hybridization technique... 74 Proteinsynthesizing systems in vitro and in vivo... 80 Directed mutagenesisin vitro GC AT transition induction... 91 Kilosequencing methods...99 Detection of DNA nucleotide sequence by Maxam and Gilbertmethod... 107 Cloning and sequencing in M13... 115 Analysis offunctional sites in genomes of pro- and eucells... 154
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Вид документа : Однотомное издание
Шифр издания : Е071/Б 63
Автор(ы) : Гительзон, Иосиф Исаевич, Волова, Татьяна Григорьевна, Дегерменджи, Андрей Георгиевич, Дегерменджи Н. Н., Шевырногов, Анатолий Петрович, Кратасюк В. А., Барцев, Сергей иванович, Болсуновский, Александр Яковлевич, Бондарь, Владимир Антонович, Буров А. Е., Величко В. В., Гладышев, Михаил Иванович, Есимбекова Е. Н., Дементьев Д. В., Есимбекова Е. Н., Задереев, Егор Сергеевич, Зотина Т. А., Косиненко, Сергей Васильевич, Медведева С. Е., Петушков В. Н., Печуркин, Николай Савельевич, Прокопкин И. Г., Пузырь А. П., Пуртов К. В., Рогозин, Денис Юрьевич, Родионова Н. С., Ронжин Н. О., Сомова, Лидия Александровна, Тихомиров, Александр Аполлинариевич, Тихомирова, Наталья Александровна, Трифонов С. В., Ушакова, Софья Аврумовна, Франк Л. А., Хромечек Е. Б., Шишацкая Е. И., Шуваев А. Н.
Заглавие : Биофизика для экологии и медицины: к 90-летию академика РАН И. И. Гительзона
Выходные данные : Новосибирск: Издательство Сибирского отделения Российской академии наук, 2019
Колич.характеристики :292, [2] с.: ил., цв. ил.; 25 см.
Коллективы : Российская академия наук. Сибирское отделение, Институт биофизики (Красноярск)
Примечания : Рез. ст. англ. - Библиогр. в конце ст.
ISBN, Цена 978-5-7692-1650-3: 1635.00 р.
ГРНТИ : 31.27 + 76.03
УДК : 577 + 574 + 61
ББК : Е071я43 + Р252.0я43
Предметные рубрики: Экологическая биофизика
Медицинская биофизика
Ключевые слова (''Своб.индексиров.''): биолюминесценция--люцифераза--целентаразин--олигохеты--замкнутые экосистемы--управляемый биосинтез--наноалмазы--радиоизотопные методы--биосфера--жирные кислоты--системы жизнеобеспечения--меромиктические озера--геобиосфера--эволюция--глобальный климат--медицинская биофизика
Содержание : Краткий очерк истории, состояния и перспектив/ И. И. Гительзон. Коллекция культур ибсо как база для исследований биолюминесценции й и грибов в ИБФ СО РАН/ С. Е. Медведева. Биолюминесценция Мирового океана/ И. И. Гительзон, Л. А. Левин, А. С. Артемкин, Р. Н., Чепилов В. В., Молвинских С.Л., Черепанов О. А., Чугунов Ю. В., Караев Н. Д., Загородний Ю. А., Шевырногов А. П. Утюшев Р. Н. Бактериальная люцифераза в биолюминесцентном анализе/ В. А. Кратасюк, Е. Н. Есимбекова. Целентеразин-зависимые биолюминесцентные системы/ Л. А. Франк. Изучение химического механизма биолюминесценции грибов/ К. В. Пуртов, В. Н. Петушков, Н. С. Родионова. Исследование биолюминесценции сибирских почвенных олигохет/ Н. С. Родионова, А. А. Петушков. Экспериментальные модели замкнутых экосистем с расчетной долей человека как перспективное направление исследований по созданию биолого-технической системы жизнеобеспечения/ А. А. Тихомиров, С. А. Ушакова, Н. А. Тихомирова, С. В., Величко В. В. Трифонов С. В. Управляемый биосинтез: от параметрически управляемых продуцирующих биосистем до новейших биофизических технологий/ Т. Г. Волова, Е. И. Шишацкая. Биомедицинские приложения наноалмазов взрывного синтеза/ В. С. Бондарь, А. П. Пузырь, Н. О. Ронжин, А. В., Буров А. Е. Барон А. В. Применение радиоизотопных методов в институте биофизики СО РАН: от клеток крови до экосистем/ А. Я. Болсуновский, С. В. Косиненко, Т. А. Зотина, Д. В. Дементьев. Биосфера - взгляд сверху (экспрессные методы мониторинга биосферы в ИБФ СО РАН – ХХ–ХХI вв.)/ А. П. Шевырногов. Жирные кислоты в экологической биофизике водных систем/ М. И. Гладышев. Сравнительное исследование устойчивости стратификации и структуры трофической сети в меромиктических озерах Шира и Шунет (Южная Сибирь, Россия)/ Д. Ю. Рогозин, Е. С. Задереев, И. Г. Прокопкин [и др.]. Непрерывный рост интенсивности энерго-вещественных взаимодействий в эволюции геобиосферы Земли/ Н. С. Печуркин, А. Н. Шуваев, Л. А. Сомова. Малоразмерные модели биосферы и феноменология изменения глобального климата/ С. И. Барцев, А. Г. Дегерменджи. Направления развития биофизики в Красноярске/ А. Г. Дегерменджи.
Аннотация: Сборник посвящен широкому кругу исследований в области экологической биофизики – научного направления на стыке наук – от исследований на молекулярном уровне до вопросов управления большими природными экосистемами. Рассмотрены исторические вехи развития экологического направления биофизики. Основной акцент сборника основан на современных, актуальных достижениях красноярских биофизиков, которым удалось сохранить и развить многоплановые направления, которые были заложены в 50-х гг. ХХ века И. И. Гительзоном. Наряду с обзорными материалами и результатами фундаментальных исследований представлен ряд готовых к внедрению биотехнологий. Книга адресована биофизикам, экологам и химикам, а также преподавателям и студентам биофизических, биологических и экологических кафедр университетов.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Soukhovolsky V. G., Ivanova Y. D., Shulman K., Mazharov V. F., Tarasova I. V., Tarasova O. V., Khlebopros R. G.
Заглавие : The population dynamics of cancer incidence: The model of a second-order phase transition
Место публикации : Biophysics. - 2015. - Vol. 60, Is. 4. - С. 639-646. - ISSN 00063509 (ISSN) , DOI 10.1134/S0006350915040235
Ключевые слова (''Своб.индексиров.''): cancer incidence--models of cancer incidence development--phase transitions of the second order
Аннотация: This paper considers an approach to the description of the age-specific and time-dependent dynamics of cancer incidence. This approach is based on a model that describes the age-specific dynamics of cancer as a phase transition of the second order and is widely used in the study of physical systems. The proposed model of cancer development as the second-order phase transitions is quite consistent with the data of medical statistics, is well described using only two free parameters, easily verified by the statistical data, and is interpreted well. The applicability of the model of second-order phase transitions to the description of the processes in the nonphysical systems is likely to be determined by the versatile nature of the processes that occur during the phase transitions. © 2015, Pleiades Publishing, Inc.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Dubovskaya, Olga P., Tang, Kam W., Gladyshev, Michail I., Kirillin, Georgiy, Buseva, Zhanna, Kasprzak, Peter, Tolomeev, Aleksandr P., Grossart, Hans-Peter
Заглавие : Estimating In Situ Zooplankton Non-Predation Mortality in an Oligo-Mesotrophic Lake from Sediment Trap Data: Caveats and Reality Check
Колич.характеристики :17 с
Коллективы : Russian Foundation for Basic Research, Belarusian Republican Foundation for Fundamental Research [14-05-90005-Bel-a, B14R-066], Humboldt Fellowship for Experienced Researchers (Germany), Russian Federal Tasks of Fundamental Research [51.1.1], German Science Foundation [GR 1540/20-1, KI-853/8-1]
Место публикации : PLoS One: PUBLIC LIBRARY SCIENCE, 2015. - Vol. 10, Is. 7. - Ст.e0131431. - ISSN 1932-6203, DOI 10.1371/journal.pone.0131431
Примечания : Cited References:60. - This work was supported by joint projects of Russian Foundation for Basic Research and Belarusian Republican Foundation for Fundamental Research (No 14-05-90005-Bel-a to O.P.D and A.P.T., and No B14R-066 to Zh.B.). K.W.T. was supported by a Humboldt Fellowship for Experienced Researchers (Germany), M.I.G. was supported by Russian Federal Tasks of Fundamental Research (project No. 51.1.1), H.P.G. and G.K. were supported by grants from the German Science Foundation (GR 1540/20-1 and KI-853/8-1).
Предметные рубрики: FRESH-WATER
NONCONSUMPTIVE MORTALITY
NONPREDATORY MORTALITY
Аннотация: Background Mortality is a main driver in zooplankton population biology but it is poorly constrained in models that describe zooplankton population dynamics, food web interactions and nutrient dynamics. Mortality due to non-predation factors is often ignored even though anecdotal evidence of non-predation mass mortality of zooplankton has been reported repeatedly. One way to estimate non-predation mortality rate is to measure the removal rate of carcasses, for which sinking is the primary removal mechanism especially in quiescent shallow water bodies. Objectives and Results We used sediment traps to quantify in situ carcass sinking velocity and non-predation mortality rate on eight consecutive days in 2013 for the cladoceran Bosmina longirostris in the oligo-mesotrophic Lake Stechlin; the outcomes were compared against estimates derived from in vitro carcass sinking velocity measurements and an empirical model correcting in vitro sinking velocity for turbulence resuspension and microbial decomposition of carcasses. Our results show that the latter two approaches produced unrealistically high mortality rates of 0.58-1.04 d(-1), whereas the sediment trap approach, when used properly, yielded a mortality rate estimate of 0.015 d(-1), which is more consistent with concurrent population abundance data and comparable to physiological death rate from the literature. Ecological implications Zooplankton carcasses may be exposed to water column microbes for days before entering the benthos; therefore, non-predation mortality affects not only zooplankton population dynamics but also microbial and benthic food webs. This would be particularly important for carbon and nitrogen cycles in systems where recurring mid-summer decline of zooplankton population due to non-predation mortality is observed.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Markova, Svetlana V., Larionova, Marina D., Burakova, Ludmila P., Vysotski, Eugene S.
Заглавие : The smallest natural high-active luciferase: Cloning and characterization of novel 16.5-kDa luciferase from copepod Metridia longa
Колич.характеристики :6 с
Коллективы : Bayer AG (Germany); Russian Science Foundation [14-14-01119]
Место публикации : Biochem. Biophys. Res. Commun.: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2015. - Vol. 457, Is. 1. - С. 77-82. - ISSN 0006-291X, DOI 10.1016/j.bbrc.2014.12.082. - ISSN 1090-2104(eISSN)
Примечания : Cited References:20. - The cloning of cDNA encoding MLuc7 luciferase of M. longa was supported by Bayer AG (Germany); all other studies - by the grant 14-14-01119 of the Russian Science Foundation. We declare that authors have no conflict of interest.
Предметные рубрики: CDNA CLONING
SECRETED LUCIFERASE
ESCHERICHIA-COLI
EXPRESSION
Ключевые слова (''Своб.индексиров.''): bioluminescence--coelenterazine--copepod luciferase--mammalian--expression--real-time imaging
Аннотация: Coelenterazine-dependent copepod luciferases containing natural signal peptide for secretion are a very convenient analytical tool as they enable monitoring of intracellular events with high sensitivity, without destroying cells or tissues. This property is well suited for application in biomedical research and development of cell-based assays for high throughput screening. We report the cloning of cDNA gene encoding a novel secreted non-allelic 16.5-kDa isoform (MLuc7) of Metridia longa luciferase, which, in fact, is the smallest natural luciferase of known for today. Despite the small size, isoform contains 10 conservative Cys residues suggesting the presence of up to 5 S-S bonds. This hampers the efficient production of functionally active recombinant luciferase in bacterial expression systems. With the use of the baculovirus expression system, we produced substantial amounts of the proper folded MLuc7 luciferase with a yield of similar to 3 mg/L of a high purity protein. We demonstrate that MLuc7 produced in insect cells is highly active and extremely thermostable, and is well suited as a secreted reporter when expressed in mammalian cells ensuring higher sensitivity of detection as compared to another Metridia luciferase isoform (MLuc164) which is widely employed in real-time imaging. (C) 2014 Elsevier Inc. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Markova S.V., Vysotski E.S.
Заглавие : Coelenterazine-dependent luciferases
Место публикации : Biochemistry Moscow. - 2015. - Vol. 80, Is. 6. - С. 714-732. - ISSN 00062979 (ISSN) , DOI 10.1134/S0006297915060073
Ключевые слова (''Своб.индексиров.''): bioluminescence--coelenterazine--luciferase--luciferin--coelenterata--cypridina luciferin--fungi--hexapoda--mollusca--protozoa
Аннотация: Bioluminescence is a widespread natural phenomenon. Luminous organisms are found among bacteria, fungi, protozoa, coelenterates, worms, molluscs, insects, and fish. Studies on bioluminescent systems of various organisms have revealed an interesting feature - the mechanisms underlying visible light emission are considerably different in representatives of different taxa despite the same final result of this biochemical process. Among the several substrates of bioluminescent reactions identified in marine luminous organisms, the most commonly used are imidazopyrazinone derivatives such as coelenterazine and Cypridina luciferin. Although the substrate used is the same, bioluminescent proteins that catalyze light emitting reactions in taxonomically remote luminous organisms do not show similarity either in amino acid sequences or in spatial structures. In this review, we consider luciferases of various luminous organisms that use coelenterazine or Cypridina luciferin as a substrate, as well as modifications of these proteins that improve their physicochemical and bioluminescent properties and therefore their applicability in bioluminescence imaging in vivo. © 2015 Pleiades Publishing, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Trifonov S. V., Kudenko Y. A., Tikhomirov A. A.
Заглавие : Bioassay of products of organic waste mineralization: An approach for closed ecosystems
Место публикации : Ecol. Eng. - 2016. - Vol. 91. - С. 139-142. - ISSN 09258574 (ISSN) , DOI 10.1016/j.ecoleng.2016.02.032
Ключевые слова (''Своб.индексиров.''): closed life support systems (clss)--organic waste--physicochemical oxidation--products of mineralization--radish growth--bioassay--electric fields--solutions--space flight--wastes--alternating current--closed life support systems (clss)--gaseous environments--high sensitivity--liquid products--organic wastes--plant productivity--products of mineralization--mineralogy--embryophyta--raphanus sativus
Аннотация: The study assesses the usability of the method of organic waste mineralization in the hydrogen peroxide aqueous solution under application of an alternating current electric field in closed life support systems (CLSS). The effects of the mineralized organic wastes on the higher plant component of the CLSS intended for space flights were studied experimentally. Radish plants, representing the higher plant compartment of the CLSS, were chosen for their high sensitivity to the pollution of the gaseous environment. The study showed that plant productivity remained comparable to that of control plants in the experiments with gaseous and liquid products of mineralization of human wastes and inedible plant parts used both separately and simultaneously. Results of the study suggest that this method is eco-friendly and suitable for use in the CLSS. © 2016 Elsevier B.V.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gribble M. O., Karimi R., Feingold B. J., Nyland J. F., O'Hara T. M., Gladyshev M. I., Chen C. Y.
Заглавие : Mercury, selenium and fish oils in marine food webs and implications for human health
Место публикации : J. Mar. Biol. Assoc. U.K. - 2016. - Vol. 96, Is. 1. - С. 43-59. - ISSN 00253154 (ISSN) , DOI 10.1017/S0025315415001356
Ключевые слова (''Своб.индексиров.''): docosahexaenoic acid--ecotoxicology--eicosapentaenoic acid--fish oils--mercury--n-3 fatty acids--oceans and human health--ohh--public health--selenium
Аннотация: Humans who eat fish are exposed to mixtures of healthful nutrients and harmful contaminants that are influenced by environmental and ecological factors. Marine fisheries are composed of a multitude of species with varying life histories, and harvested in oceans, coastal waters and estuaries where environmental and ecological conditions determine fish exposure to both nutrients and contaminants. Many of these nutrients and contaminants are thought to influence similar health outcomes (i.e., neurological, cardiovascular, immunological systems). Therefore, our understanding of the risks and benefits of consuming seafood require balanced assessments of contaminants and nutrients found in fish and shellfish. In this paper, we review some of the reported benefits of fish consumption with a focus on the potential hazards of mercury exposure, and compare the environmental variability of fish oils, selenium and mercury in fish. A major scientific gap identified is that fish tissue concentrations are rarely measured for both contaminants and nutrients across a range of species and geographic regions. Interpreting the implications of seafood for human health will require a better understanding of these multiple exposures, particularly as environmental conditions in the oceans change. © Marine Biological Association of the United Kingdom, 2015.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Trifonov S. V., Kudenko Y. A., Tikhomirov A. A.
Заглавие : Prospects for using a full-scale installation for wet combustion of organic wastes in closed life support systems
Место публикации : Life Sci. Space Res. - 2015. - Vol. 7. - С. 15-21. - ISSN 22145524 (ISSN) , DOI 10.1016/j.lssr.2015.08.003
Ключевые слова (''Своб.индексиров.''): automatic control--human waste--life support system--physicochemical oxidation--radish growth--hydrogen peroxide--alternating current--article--closed life support system--combustion--control system--electric field--electricity--human--microclimate--mineralization--organic waste--oxidation--radish--recycling--scale up--waste--wet combustion
Аннотация: The issue of recycling organic wastes in closed life support systems (CLSS) includes both fundamental aspects of environmental safety of the recycled products and their effective involvement in material cycles and technical aspects related to the structure of the system and the crew's demands. This study estimates the effectiveness of wet combustion of different amounts of organic wastes in hydrogen peroxide under application of an alternating current electric field. The study also addresses the possibility of controlling the process automatically. The results show that processing of greater amounts of wastes reduces specific power consumption and shortens the duration of the process, without significantly affecting the level of oxidation of the products. An automatic control system for a semi-commercial installation has been constructed and tested experimentally. The solution of mineralized human wastes prepared in the automatically controlled process in this installation was successfully used to grow radish plants, with the main production parameters being similar to those of the control. © 2015 The Committee on Space Research (COSPAR). Published by Elsevier Ltd. All rights reserved.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kratasyuk, Valentina A., Esimbekova, Elena N.
Заглавие : Applications of Luminous Bacteria Enzymes in Toxicology
Колич.характеристики :8 с
Коллективы : Russian Science Foundation [15-19-10041]
Место публикации : Comb. Chem. High Throughput Screen: BENTHAM SCIENCE PUBL LTD, 2015. - Vol. 18, Is. 10. - С. 952-959. - ISSN 1386-2073, DOI 10.2174/1386207318666150917100257. - ISSN 1875-5402(eISSN)
Примечания : Cited References:88. - The research was supported by the Russian Science Foundation, project No. 15-19-10041.
Предметные рубрики: NADHFMN-OXIDOREDUCTASE-LUCIFERASE
HUMIC SUBSTANCES
BIOLUMINESCENT
Ключевые слова (''Своб.индексиров.''): bioluminescence--bioluminescent toxicity enzymatic assay--immobilization--of enzymes--luciferase--total toxicity
Аннотация: This review describes the principle and applications of bioluminescent enzymatic toxicity bioassays. This type of assays uses bacterial coupled enzyme systems: NADH: FMN-oxidoreductase and luciferase to replace living organisms in developing cost-competitive biosensors for environmental, medical and industrial applications. These biosensors instantly signal chemical and biological hazards and allow for detecting a great amount of toxic compounds with advantages associated with fast results, high sensitivity, simplicity, low cost and safety of the procedure.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gaevsky N.A., Kolmakov V.I., Anishchenko O.V., Gorbaneva T.B.
Заглавие : Using DCMU-fluorescence method for the identification of dominant phytoplankton groups
Колич.характеристики :12 с
Место публикации : J. Appl. Phycol.: SPRINGER, 2005. - Vol. 17, Is. 6. - P483-494. - ISSN 0921-8971, DOI 10.1007/s10811-005-2903-x
Примечания : Cited References: 28
Предметные рубрики: IN-VIVO
CHLOROPHYLL FLUORESCENCE
FLUOROMETRIC METHOD
SITU
PHOTOSYNTHESIS
CYANOBACTERIA
ALGAE
EVOLUTION
CULTURES
LAKES
Ключевые слова (''Своб.индексиров.''): algae--chlorophyll--fluorescence--vizualization
Аннотация: For the identification of ecologically significant dominant groups of phytoplanktonic algae a polychromatic DCMU-induced fluorescence method is recommended. A special fluorometer equipped with a system of replaceable filters is used to differentiate three regions of the spectrum (410 +/- 20, 510 +/- 20 and 540 +/- 10 nm) that can excite the basic light-harvesting pigments. Total and differential (for every algal taxon studied) chlorophyll a calculated from the fluorescence signals is in good agreement with biomass estimates from direct cell counts for several different trophic types of aquatic systems. This is made possible by the vizualization of the ratios of fluorescence signal values in their own coordinates: first, to decide whether it is necessary to correct linear equations in order to eliminate negative solutions; second, to determine the possibility of nulling the negative solution if a point is situated close to a side of the triangle; and third, to reduce the number of linear algebraic equations to two if the points are situated along one of the triangle sides or to one if the points are gathered at the apex. The polychromatic DCMU-induced fluorescence method can be used for monitoring natural phytoplankton populations to detect changes in their taxonomic structure.
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12.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Esimbekova E.N., Kratasyuk V.A.
Заглавие : Immobilization of bioluminescent systems and their applications
Колич.характеристики :4 с
Место публикации : Bioluminescence & Chemiluminescence: Progress and Perspectives: WORLD SCIENTIFIC PUBL CO PTE LTD, 2005. - 13th International Symposium on Bioluminescence and Chemiluminescence (AUG 02-06, 2004, Yokohama, JAPAN). - P237-240. - ISBN 981-256-118-8, DOI 10.1142/9789812702203_0055
Примечания : Cited References: 14
Предметные рубрики: ACIDS
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13.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Shevyrnogov A..., Vysotskaya G..., Shevyrnogov E...
Заглавие : Results of analysis of spatial and temporal long-term stability of quasistationary areas in the Atlantic Ocean based on multi-satellite measurements (CZCS and SeaWiFS data)
Колич.характеристики :5 с
Место публикации : CLIMATE CHANGE PROCESSES IN THE STRATOSPHERE, EARTH-ATMOSPHERE-OCEAN SYSTEMS, AND OCEANOGRAPHIC PROCESSES FROM SATELLITE DATA. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON-ELSEVIER SCIENCE LTD, 2004. - Vol. 33: 2nd World Space Congress/34th COSPAR Scientific Assembly (OCT 10-19, 2002, HOUSTON, TX), Is. 7. - P1184-1188. - ISBN 0273-1177, DOI 10.1016/S0273-1177(03)00366-1
Примечания : Cited References: 6
Ключевые слова (''Своб.индексиров.''): chlorophyll--seasonal dynamics--hydrological structure--space-based data
Аннотация: The paper analyzes the stability of areas in the Global Ocean, which are quasistationary with respect to the seasonal dynamics of chlorophyll concentration. Such areas were first discovered based on the CZCS data. They occur almost in all regions of the Global Ocean. They reflect typical seasonal dynamics of phytoplankton, which depends on both large ocean currents and local hydrological instability. It is related to the displacement of frontal zones and to local hydrological anomalies. Since the completion of the CZCS work there has been a 10 years gap in monitoring chlorophyll spatial distribution dynamics in the ocean. The reason is the long absence of equipment on the orbit, which would be suitable for the measurement of chlorophyll concentration. Recently, SeaWiFS data have been accumulated and their quantity is sufficient for the crude statistical analysis of image time series for 25 years. Thus, it has become possible to obtain data on the long-term stability of quasistationary areas and, therefore, on the stability of large-scale hydrological phenomena in the Atlantic Ocean. (C) 2003 COSPAR. Published by Elsevier Ltd. All rights reserved.
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14.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Bartsev S.I.
Заглавие : Stoichiometric constraints and complete closure of long-term life support systems
Колич.характеристики :8 с
Место публикации : SPACE LIFE SCIENCES: LIFE SUPPORT SYSTEMS AND BIOLOGICAL SYSTEMS UNDER INFLUENCE OF PHYSICAL FACTORS. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON-ELSEVIER SCIENCE LTD, 2004. - Vol. 34: 2nd World Space Congress/34th COSPAR Scientific Assembly (OCT 10-19, 2002, HOUSTON, TX), Is. 7. - P1509-1516. - ISBN 0273-1177, DOI 10.1016/j.asr.2003.04.069
Примечания : Cited References: 14
Ключевые слова (''Своб.индексиров.''): long-term life support systems--stoichiometric constraints
Аннотация: High closure of matter recycling is an obvious requirement for long-term life support systems (LSS). Biological species are obligate components of the LSS since physical/chemical components are not able yet to provide food for crew. However including biological species into LSS is difficult due to specific stoichiometric configuration of their inputs and outputs. Formally the problem is to estimate the ability for given set of species to provide complete closure of LSS. Two possible models of metabolism organization can be considered: rigid and flexible ones. Stoichiometric analyses showed that the rigid metabolism case is not typical and takes place with very specific requirements. The flexible metabolic model can be applied to describing wide range of systems. Some formal indications of ability to provide complete closure and stationarity of LSS state are considered in the paper. These indications establish some constraints on the form of mathematical models intended to describe artificial and natural ecological systems. (C) 2004 COSPAR. Published by Elsevier Ltd. All rights reserved.
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15.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Pisman T.I., Galayda Y.V., Shirobokova I.M.
Заглавие : Experimental and mathematical modeling of the consumer's influence on productivity of algae in a model aquatic ecosystem
Колич.характеристики :7 с
Место публикации : SPACE LIFE SCIENCES: CLOSED ECOLOGICAL SYSTEMS: EARTH AND SPACE APPLICATIONS. Ser. ADVANCES IN SPACE RESEARCH-SERIES: PERGAMON-ELSEVIER SCIENCE LTD, 2005. - Vol. 35: Workshop on Closed Ecological Systems (JUL, 2004, Paris, FRANCE), Is. 9. - P1521-1527. - ISBN 0273-1177, DOI 10.1016/j.asr.2004.12.048
Примечания : Cited References: 10
Ключевые слова (''Своб.индексиров.''): producer--consumer--nitrogen cycling--mathematical modeling
Аннотация: A "producer-consumer" (Chlorella vulgaris-Paramecium caudatum) closed aquatic system has been investigated experimentally and theoretically. It has been found that there is a direct relationship between the growth of the paramecia population and their release of ammonia nitrogen, which is the best form of nitrogen for Chlorella growth. The theoretical study of a model of a "producer-consumer" aquatic biotic cycle with spatially separated compartments has confirmed the contribution of paramecia to nitrogen cycling. It has been shown that an increase in the concentration of nitrogen released as metabolites of paramecia is accompanied by an increase in the productivity of microalgae. (c) 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.
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16.

Вид документа : Статья из сборника (выпуск монографической серии)
Шифр издания :
Автор(ы) : Frank, Ludmila A., Krasitskaya, Vasilisa V.
Заглавие : Application of Enzyme Bioluminescence for Medical Diagnostics
Колич.характеристики :23 с
Место публикации : Adv. Biochem. Eng. Biotechnol.: SPRINGER-VERLAG BERLIN, 2014. - Vol. 144. - С. 175-197. - (Advances in Biochemical Engineering-Biotechnology). - , DOI 10.1007/978-3-662-43385-0_6
Примечания : Cited References:63
Предметные рубрики: RESONANCE ENERGY-TRANSFER
POLYMERASE-CHAIN-REACTION
LUCIFERASE
Ключевые слова (''Своб.индексиров.''): bioluminescence--ca2+-regulated photoprotein--diagnostics--immunoassay--luciferase--nucleic acid hybridization assay
Аннотация: Nowadays luciferases are effectively used as analytical instruments in a great variety of research fields. Of special interest are the studies dealing with elaboration of novel analytical systems for the purposes of medical diagnostics. The ever-expanding spectrum of clinically important analytes accounts for the increasing demand for new techniques for their detection. In this chapter we have made an attempt to summarize the results on applications of luciferases as reporters in binding assays including immunoassay, nucleic acid hybridization assay, and so on. The data over the last 15 years have been analyzed and clearly show that luciferase-based assays, due to extremely high sensitivity, low cost, and the lack of need for skilled personnel, hold much promise for clinical diagnostics.
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17.

Вид документа : Статья из сборника (выпуск монографической серии)
Шифр издания :
Автор(ы) : Esimbekova, Elena, Kratasyuk, Valentina, Shimomura, Osamu
Заглавие : Application of Enzyme Bioluminescence in Ecology
Колич.характеристики :43 с
Место публикации : Adv. Biochem. Eng. Biotechnol.: SPRINGER-VERLAG BERLIN, 2014. - Vol. 144. - С. 67-109. - (Advances in Biochemical Engineering-Biotechnology). - , DOI 10.1007/978-3-662-43385-0_3
Примечания : Cited References:85
Предметные рубрики: BACTERIAL LUCIFERASE
IN-VITRO
PYRETHROID INSECTICIDES
FRESH-WATER
Ключевые слова (''Своб.индексиров.''): bioluminescence--ecological monitoring--enzymatic assay--immobilization--integral water toxicity--luciferase
Аннотация: This review examines the general principles of bioluminescent enzymatic toxicity bioassays and describes the applications of these methods and the implementation in commercial biosensors. Bioluminescent enzyme system technology (BEST) has been proposed in the bacterial coupled enzyme system, wherein NADH: FMN-oxidoreductase-luciferase substitutes for living organisms. BEST was introduced to facilitate and accelerate the development of cost-competitive enzymatic systems for use in biosensors for medical, environmental, and industrial applications. For widespread use of BEST, the multicomponent reagent "Enzymolum'' has been developed, which contains the bacterial luciferase, NADH: FMN-oxidoreductase, and their substrates, co-immobilized in starch or gelatin gel. Enzymolum is the central part of Portable Laboratory for Toxicity Detection (PLTD), which consists of a biodetector module, a sampling module, a sample preparation module, and a reagent module. PLTD instantly signals chemical-biological hazards and allows us to detect a wide range of toxic substances. Enzymolum can be integrated as a biological module into the portable biodetector-biosensor originally constructed for personal use. Based on the example of Enzymolum and the algorithm for creating new enzyme biotests with tailored characteristics, a new approach was demonstrated in biotechnological design and construction. The examples of biotechnological design of various bioluminescent methods for ecological monitoring were provided. Possible applications of enzyme bioassays are seen in the examples for medical diagnostics, assessment of the effect of physical load on sportsmen, analysis of food additives, and in practical courses for higher educational institutions and schools. The advantages of enzymatic assays are their rapidity (the period of time required does not exceed 3-5 min), high sensitivity, simplicity and safety of procedure, and possibility of automation of ecological monitoring; the required luminometer is easily available.
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18.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Bartsev S.I., Mezhevikin V.V.
Заглавие : Pre-biotic stage of life origin under non-photo synthetic conditions
Колич.характеристики :5 с
Место публикации : SPACE LIFE SCIENCES: CLOSED ECOLOGICAL SYSTEMS: EARTH AND SPACE APPLICATIONS. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON-ELSEVIER SCIENCE LTD, 2005. - Vol. 35: Workshop on Closed Ecological Systems (JUL, 2004, Paris, FRANCE), Is. 9. - P1643-1647. - ISBN 0273-1177, DOI 10.1016/j.asr.2005.04.072
Примечания : Cited References: 14
Ключевые слова (''Своб.индексиров.''): life origin--pre-biotic autocatalytic system--phase-separated autocatalytic particles--multivariate oligomeric autocatalyst
Аннотация: Spontaneous assembling of a simplest bacterial cell even if all necessary molecules are present in a solution seems to be extremely rare event and from the scientific standpoint has to be considered as impossible. Therefore, a predecessor of a living cell has to be very simple for providing its self-assembling and at the same time it should be able of progressive increase in complexity. Now phase-separated particles, first of all micelles, are put forward as possible predecessors of living cell. According to the offered working concept only phase-separated particles possessing autocatalytic properties can be considered as predecessors of living cells. The first stage of evolution of these phase-separated autocatalytic systems is the appearance of pre-biotic metabolism providing synthesis of amphiphiles for formation of capsules of these systems. This synthesis is maintained by the energy of a base reaction being a component of a planet-chemical cycle. Catalytic system providing functioning of pre-biotic metabolism is based on multivariate oligomeric autocatalyst, which reproduces itself from monomers, penetrating the particles from the outside. Since the autocatalyst realizes random polymerization then a collection of other oligomers possessing different catalytic functions is produced. In the paper the functioning of multivariate oligomeric autocatalyst in flow reactor is analyzed. (c) 2005 Published by Elsevier Ltd on behalf of COSPAR.
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19.

Вид документа : Статья из сборника (выпуск продолж. издания)
Шифр издания :
Автор(ы) : Kokova V.E., Spitskaya N.I.
Заглавие : CONTINUOUS CULTIVATION OF SOME WATER INVERTEBRATES
Место публикации :. - 1984. - Third European Congress on Biotechnology., Munich, W Ger) Conference code: 6973. - P539-543
Ключевые слова (''Своб.индексиров.''): marine biology--artificial ecological systems--continuous cultivation--population growth rate--range conditions--suspended nutrients--water invertebrates--ecology
Аннотация: Continuous cultivation of water invertebrates facilitates the study of their biological characteristics and productivity and hence contributes to creation of artifical ecological systems. When applying the technique, suspended nutrients are being passed through the cultivator and culture is outflowing continuously, with the volume inside the vessel being constant. The objective of the study is to examine the effect of a range conditions of cultivating on population growth rate.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kratasyuk V. A., Esimbekova E. N.
Заглавие : Applications of luminous bacteria enzymes in toxicology
Место публикации : Comb. Chem. High Throughput Screen. - 2015. - Vol. 18, Is. 10. - С. 952-959. - ISSN 13862073 (ISSN)
Ключевые слова (''Своб.индексиров.''): bioluminescence--bioluminescent toxicity enzymatic assay--immobilization of enzymes--luciferase--total toxicity
Аннотация: This review describes the principle and applications of bioluminescent enzymatic toxicity bioassays. This type of assays uses bacterial coupled enzyme systems: NADH:FMN-oxidoreductase and luciferase to replace living organisms in developing cost-competitive biosensors for environmental, medical and industrial applications. These biosensors instantly signal chemical and biological hazards and allow for detecting a great amount of toxic compounds with advantages associated with fast results, high sensitivity, simplicity, low cost and safety of the procedure. © 2015 Bentham Science Publishers.
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