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Вид документа : Статья из сборника (однотомник)
Шифр издания : Е071/Б 63
Автор(ы) : Франк Л. А.
Заглавие : Целентеразин-зависимые биолюминесцентные системы
Параллельн. заглавия :Coelenterazine-dependent bioluminescent systems
Коллективы : Российская академия наук, Институт биофизики
Место публикации : Биофизика для экологии и медицины: к 90-летию академика РАН И. И. Гительзона/ И. И. Гительзон, Т. Г. Волова, А. Г. Дегерменджи [и др.] ; ред., авт. предисл. Т. Г. Волова. - Новосибирск: Издательство Сибирского отделения Российской академии наук, 2019. - С. 72-87. - ISBN 978-5-7692-1650-3 (Шифр Е071/Б 63-478048446)
Примечания : Библиогр.: с. 85-87
УДК : 577 + 574 + 61
ББК : Е071я43 + Р252.0я43
Ключевые слова (''Своб.индексиров.''): люцифераза
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Вид документа : Однотомное издание
Шифр издания : Е041/М 54
Автор(ы) : Мазин, Александр Владимирович, Кузнеделов К. Д., Краев А. С., Холодилов Н. Г.
Заглавие : Методы молекулярной генетики и генной инженерии : научное издание
Выходные данные : Новосибирск: Наука. Сиб. отд-ние, 1990
Колич.характеристики :247 с.
Коллективы : АН СССР. Сибирское отделение, Институт цитологии и генетики (Новосибирск)
Примечания : Библиогр. в конце глав. - Авт. указаны на обороте тит. л.
ISBN, Цена 5-02-029565-5: 3.10, 3.10, р.
ГРНТИ : 34.15.27 + 34.15.23 + 34.23.19
УДК : 577.21.08
ББК : Е041.10в7 + Е041.15в7
Предметные рубрики: Молекулярная генетика
Генная инженерия
Аннотация: Выделение ДНК... 7 Эндонуклеазы рестрикции II типа: свойства иприменение... 14 Введение метки в ДНК... 25 Блот-гибридизация ДНК накапроновых мембранах... 35 Трансформация E. coli плазмидной ДНК... 39Коннекторный способ клонирования кДНК... 44 Получение геномныхбиблиотек в -векторах... 56 Детекция рекомбинантной ДНК методоммолекулярной гибридизации... 74 Белоксинтезирующие системы in vitro иin vivo... 80 Направленный мутагенез in vitro. Индукция транзиций GC AT... 91 Метод килосеквенирования... 99 Определение нуклеотиднойпоследовательности ДНК методом Максама-Гилберта... 107 Клонирование исеквенирование в М13... 115 Анализ функциональных сайтов в геномахпро- и эукариот... 154DNA separation... 7 Type II restriction endonuclease:properties and application... 14 DNA labelling... 25 DNA blothybridization on kapron membranes... 35 E. coli plasmid DNAtransformation... 39 Connection method of cDNA cloning... 44Producing genomic libraries in lambda-vectors... 56 Recombinant DNAdetection by molecular hybridization technique... 74 Proteinsynthesizing systems in vitro and in vivo... 80 Directed mutagenesisin vitro GC AT transition induction... 91 Kilosequencing methods...99 Detection of DNA nucleotide sequence by Maxam and Gilbertmethod... 107 Cloning and sequencing in M13... 115 Analysis offunctional sites in genomes of pro- and eucells... 154
Экземпляры :КФ(1)
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Вид документа : Однотомное издание
Шифр издания : Е071/Б 63
Автор(ы) : Гительзон, Иосиф Исаевич, Волова, Татьяна Григорьевна, Дегерменджи, Андрей Георгиевич, Дегерменджи Н. Н., Шевырногов, Анатолий Петрович, Кратасюк В. А., Барцев, Сергей иванович, Болсуновский, Александр Яковлевич, Бондарь, Владимир Антонович, Буров А. Е., Величко В. В., Гладышев, Михаил Иванович, Есимбекова Е. Н., Дементьев Д. В., Есимбекова Е. Н., Задереев, Егор Сергеевич, Зотина Т. А., Косиненко, Сергей Васильевич, Медведева С. Е., Петушков В. Н., Печуркин, Николай Савельевич, Прокопкин И. Г., Пузырь А. П., Пуртов К. В., Рогозин, Денис Юрьевич, Родионова Н. С., Ронжин Н. О., Сомова, Лидия Александровна, Тихомиров, Александр Аполлинариевич, Тихомирова, Наталья Александровна, Трифонов С. В., Ушакова, Софья Аврумовна, Франк Л. А., Хромечек Е. Б., Шишацкая Е. И., Шуваев А. Н.
Заглавие : Биофизика для экологии и медицины: к 90-летию академика РАН И. И. Гительзона
Выходные данные : Новосибирск: Издательство Сибирского отделения Российской академии наук, 2019
Колич.характеристики :292, [2] с.: ил., цв. ил.; 25 см.
Коллективы : Российская академия наук. Сибирское отделение, Институт биофизики (Красноярск)
Примечания : Рез. ст. англ. - Библиогр. в конце ст.
ISBN, Цена 978-5-7692-1650-3: 1635.00 р.
ГРНТИ : 31.27 + 76.03
УДК : 577 + 574 + 61
ББК : Е071я43 + Р252.0я43
Предметные рубрики: Экологическая биофизика
Медицинская биофизика
Ключевые слова (''Своб.индексиров.''): биолюминесценция--люцифераза--целентаразин--олигохеты--замкнутые экосистемы--управляемый биосинтез--наноалмазы--радиоизотопные методы--биосфера--жирные кислоты--системы жизнеобеспечения--меромиктические озера--геобиосфера--эволюция--глобальный климат--медицинская биофизика
Содержание : Краткий очерк истории, состояния и перспектив/ И. И. Гительзон. Коллекция культур ибсо как база для исследований биолюминесценции й и грибов в ИБФ СО РАН/ С. Е. Медведева. Биолюминесценция Мирового океана/ И. И. Гительзон, Л. А. Левин, А. С. Артемкин, Р. Н., Чепилов В. В., Молвинских С.Л., Черепанов О. А., Чугунов Ю. В., Караев Н. Д., Загородний Ю. А., Шевырногов А. П. Утюшев Р. Н. Бактериальная люцифераза в биолюминесцентном анализе/ В. А. Кратасюк, Е. Н. Есимбекова. Целентеразин-зависимые биолюминесцентные системы/ Л. А. Франк. Изучение химического механизма биолюминесценции грибов/ К. В. Пуртов, В. Н. Петушков, Н. С. Родионова. Исследование биолюминесценции сибирских почвенных олигохет/ Н. С. Родионова, А. А. Петушков. Экспериментальные модели замкнутых экосистем с расчетной долей человека как перспективное направление исследований по созданию биолого-технической системы жизнеобеспечения/ А. А. Тихомиров, С. А. Ушакова, Н. А. Тихомирова, С. В., Величко В. В. Трифонов С. В. Управляемый биосинтез: от параметрически управляемых продуцирующих биосистем до новейших биофизических технологий/ Т. Г. Волова, Е. И. Шишацкая. Биомедицинские приложения наноалмазов взрывного синтеза/ В. С. Бондарь, А. П. Пузырь, Н. О. Ронжин, А. В., Буров А. Е. Барон А. В. Применение радиоизотопных методов в институте биофизики СО РАН: от клеток крови до экосистем/ А. Я. Болсуновский, С. В. Косиненко, Т. А. Зотина, Д. В. Дементьев. Биосфера - взгляд сверху (экспрессные методы мониторинга биосферы в ИБФ СО РАН – ХХ–ХХI вв.)/ А. П. Шевырногов. Жирные кислоты в экологической биофизике водных систем/ М. И. Гладышев. Сравнительное исследование устойчивости стратификации и структуры трофической сети в меромиктических озерах Шира и Шунет (Южная Сибирь, Россия)/ Д. Ю. Рогозин, Е. С. Задереев, И. Г. Прокопкин [и др.]. Непрерывный рост интенсивности энерго-вещественных взаимодействий в эволюции геобиосферы Земли/ Н. С. Печуркин, А. Н. Шуваев, Л. А. Сомова. Малоразмерные модели биосферы и феноменология изменения глобального климата/ С. И. Барцев, А. Г. Дегерменджи. Направления развития биофизики в Красноярске/ А. Г. Дегерменджи.
Аннотация: Сборник посвящен широкому кругу исследований в области экологической биофизики – научного направления на стыке наук – от исследований на молекулярном уровне до вопросов управления большими природными экосистемами. Рассмотрены исторические вехи развития экологического направления биофизики. Основной акцент сборника основан на современных, актуальных достижениях красноярских биофизиков, которым удалось сохранить и развить многоплановые направления, которые были заложены в 50-х гг. ХХ века И. И. Гительзоном. Наряду с обзорными материалами и результатами фундаментальных исследований представлен ряд готовых к внедрению биотехнологий. Книга адресована биофизикам, экологам и химикам, а также преподавателям и студентам биофизических, биологических и экологических кафедр университетов.
Экземпляры :ИБФ-КФ(1)
Свободны : ИБФ-КФ(1)
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Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Петушков В.Н., Шефер Л.П., Родионова Н.С.
Заглавие : Биолюминесцентный анализ НАДН-зависимых дегидрогеназ : научное издание
Место публикации : Получ. и применение биокатализаторов в нар. х-ве и мед. Тез. докл. 5 Всес. симп. по инж. энзимол., Кобулети, май, 1985. Т. 1. - Олайне, 1985. - С. 168
ГРНТИ : 34.15.19
Предметные рубрики: НАДНДЕГИДРОГЕНАЗА
ОПРЕДЕЛЕНИЕ
БИОЛЮМИНЕСЦЕНТНЫЙ МЕТОД
НАДН-ФМНОКСИДОРЕДУКТАЗА
ЛЮЦИФЕРАЗА
БИОЛЮМИНЕСЦЕНТНАЯ СИСТЕМА
ENZYME SYSTEMS
ANALYTICAL USES
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Neubauer, Darshan, Kolmakova, Olesya, Woodhouse, Jason, Taube, Robert, Mangelsdorf, Kai, Gladyshev, Michail, Premke, Katrin, Grossart, Hans-Peter
Заглавие : Zooplankton carcasses stimulate microbial turnover of allochthonous particulate organic matter
Колич.характеристики :16 с
Коллективы : Projekt DEAL
Место публикации : ISME J.: SPRINGERNATURE, 2021. - Article in press. - ISSN 1751-7362, DOI 10.1038/s41396-020-00883-w. - ISSN 1751-7370(eISSN)
Примечания : Cited References:83. - Open Access funding enabled and organized by Projekt DEAL.
Предметные рубрики: FRESH-WATER
SEASONAL-CHANGES
CARBON
LAKE
DECOMPOSITION
DEGRADATION
Аннотация: Carbon turnover in aquatic environments is dependent on biochemical properties of organic matter (OM) and its degradability by the surrounding microbial community. Non-additive interactive effects represent a mechanism where the degradation of biochemically persistent OM is stimulated by the provision of bioavailable OM to the degrading microbial community. Whilst this is well established in terrestrial systems, whether it occurs in aquatic ecosystems remains subject to debate. We hypothesised that OM from zooplankton carcasses can stimulate the degradation of biochemically persistent leaf material, and that this effect is influenced by the daphnia:leaf OM ratio and the complexity of the degrading microbial community. Fresh Daphnia magna carcasses and C-13-labelled maize leaves (Zea mays) were incubated at different ratios (1:1, 1:3 and 1:5) alongside either a complex microbial community (50 mu m) or solely bacteria (0.8 mu m). C-13 stable-isotope measurements of CO2 analyses were combined with phospholipid fatty acids (PLFA) analysis and DNA sequencing to link metabolic activities, biomass and taxonomic composition of the microbial community. Our experiments indicated a significantly higher respiration of leaf-derived C when daphnia-derived OM was most abundant (i.e. daphnia:leaf OM ratio of 1:1). This process was stronger in a complex microbial community, including eukaryotic microorganisms, than a solely bacterial community. We concluded that non-additive interactive effects were a function of increased C-N chemodiversity and microbial complexity, with the highest net respiration to be expected when chemodiversity is high and the degrading community complex. This study indicates that identifying the interactions and processes of OM degradation is one important key for a deeper understanding of aquatic and thus global carbon cycle.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tang K.W., Gladyshev M.I., Dubovskaya O.P., Kirillin G..., Grossart H.P.
Заглавие : Zooplankton carcasses and non-predatory mortality in freshwater and inland sea environments
Колич.характеристики :16 с
Коллективы : Humboldt Foundation; Ministry of Education and Science of Russian Federation (Siberian Federal University) [B-15]; Leibniz-Association [SAW-2011-IGB-2]; German Science foundation [KI-853/7-1, GR1540/20-1]
Место публикации : J. Plankton Res.: OXFORD UNIV PRESS, 2014. - Vol. 36, Is. 3. - С. 597-612. - ISSN 0142-7873, DOI 10.1093/plankt/fbu014. - ISSN 1464-3774
Примечания : Cited References: 168. - This work was supported by the Humboldt Foundation (Fellowship for Experienced Researchers to K. W. T.); Siberian Branch of Russian Academy of Science (Partner Project No. 8 to M. I. G. and O.P.D.); Federal Tasks of Ministry of Education and Science of Russian Federation (project B-15 of Siberian Federal University to M. I. G.); the Leibniz-Association (SAW-2011-IGB-2 to G. K.); and the German Science foundation (KI-853/7-1 to G. K., GR1540/20-1 to H. P. G.). The manuscript benefited from the constructive comments from three reviewers.
Предметные рубрики: SMALL-SCALE TURBULENCE
NON-CALANOID COPEPODS
AGGREGATES LAKE SNOW
DAPHNIA-GALEATA
MIDSUMMER DECLINE
NONCONSUMPTIVE MORTALITY
CRUSTACEAN ZOOPLANKTON
CLIMATE-CHANGE
VERTICAL-DISTRIBUTION
POPULATION-GROWTH
Ключевые слова (''Своб.индексиров.''): carbon flux--inland waters--lakes--live--dead sorting--non-predatory mortality--zooplankton carcasses
Аннотация: Zooplankton carcasses are ubiquitous in marine and freshwater systems, implicating the importance of non-predatory mortality, but both are often overlooked in ecological studies compared with predatory mortality. The development of several microscopic methods allows the distinction between live and dead zooplankton in field samples, and the reported percentages of dead zooplankton average 11.6 (minimum) to 59.8 (maximum) in marine environments, and 7.4 (minimum) to 47.6 (maximum) in fresh and inland waters. Common causes of non-predatory mortality among zooplankton include senescence, temperature change, physical and chemical stresses, parasitism and food-related factors. Carcasses resulting from non-predatory mortality may undergo decomposition leading to an increase in microbial production and a shift in microbial composition in the water column. Alternatively, sinking carcasses may contribute significantly to vertical carbon flux especially outside the phytoplankton growth seasons, and become a food source for the benthos. Global climate change is already altering freshwater ecosystems on multiple levels, and likely will have significant positive or negative effects on zooplankton non-predatory mortality. Better spatial and temporal studies of zooplankton carcasses and non-predatory mortality rates will improve our understanding of this important but under-appreciated topic.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Diaz A. V., Ageev, Aleksander Vladimirovich, Ivanova, Elena Anatolevna, Anishchenko, Olesia Valerevna
Заглавие : Winter atmospheric nutrient and pollutant deposition on Western Sayan Mountain lakes (Siberia)
Колич.характеристики :18 с
Коллективы : Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [20-04-00960]; Ministry of Science and Higher Education of the Russian Federation [5-100, FSRZ-2020-0014]
Место публикации : Biogeosciences: COPERNICUS GESELLSCHAFT MBH, 2021. - Vol. 18, Is. 5. - С. 1601-1618. - ISSN 1726-4170, DOI 10.5194/bg-18-1601-2021. - ISSN 1726-4189(eISSN)
Примечания : Cited References:86. - This research has been supported by the Russian Foundation for Basic Research (grant no. 20-04-00960) and the Ministry of Science and Higher Education of the Russian Federation (postdoctoral program project no. 5-100, grant no. FSRZ-2020-0014).
Предметные рубрики: FLY-ASH PARTICLES
NITROGEN DEPOSITION
PHOSPHORUS DEPOSITION
Аннотация: The world map of anthropogenic atmospheric nitrogen deposition and its effects on natural ecosystems is not described with equal precision everywhere. In this paper, we report atmospheric nutrient, sulfate and spheroidal carbonaceous particle (SCP) deposition rates, based on snowpack analyses of a formerly unexplored Siberian mountain region. Then, we discuss their potential effects on lake phytoplankton biomass limitation. We estimate that the nutrient depositions observed in the late-season snowpack (40 +/- 16 mgNO(3)-Nm(-2) and 0.58 +/- 0.13 mg TP-Pm-2; TP for total phosphorous) would correspond to yearly depositions lower than 119 +/- 71 mgNO(3)-Nm(-2) yr(-1) and higher than 1.71 +/- 0.91 mg TP-Pm-2 yr(-1). These yearly deposition estimates would approximately fit the predictions of global deposition models and correspond to the very low nutrient deposition range, although they are still higher than world background values. In spite of the fact that such a low atmospheric nitrogen deposition rate would be enough to induce nitrogen limitation in unproductive mountain lakes, phosphorus deposition was also extremely low, and the resulting lake water N: P ratio was unaffected by atmospheric nutrient deposition. In the end, the studied lakes' phytoplankton appeared to be split between phosphorus and nitrogen limitation. We conclude that these pristine lakes are fragile sensitive systems exposed to the predicted climate warming, increased winter precipitation, enhanced forest fires and shifts in anthropogenic nitrogen emissions that could finally couple their water chemistry to that of atmospheric nutrient deposition and unlock temperature-inhibited responses of phytoplankton to nutrient shifts.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Diaz-De-Quijano D., Vladimirovich Ageev A., Anatolevna Ivanova E., Valerevna Anishchenko O.
Заглавие : Winter atmospheric nutrient and pollutant deposition on Western Sayan Mountain lakes (Siberia)
Место публикации : Biogeosciences: Copernicus GmbH, 2021. - Vol. 18, Is. 5. - С. 1601-1618. - ISSN 17264170 (ISSN), DOI 10.5194/bg-18-1601-2021
Аннотация: The world map of anthropogenic atmospheric nitrogen deposition and its effects on natural ecosystems is not described with equal precision everywhere. In this paper, we report atmospheric nutrient, sulfate and spheroidal carbonaceous particle (SCP) deposition rates, based on snowpack analyses of a formerly unexplored Siberian mountain region. Then, we discuss their potential effects on lake phytoplankton biomass limitation. We estimate that the nutrient depositions observed in the late-season snowpack (4016 mgNO3-Nm2 and 0.580.13 mg TP-Pm2; TP for total phosphorous) would correspond to yearly depositions lower than 11971 mgNO3-Nm2 yr1 and higher than 1.710.91 mg TP-Pm2 yr1. These yearly deposition estimates would approximately fit the predictions of global deposition models and correspond to the very low nutrient deposition range, although they are still higher than world background values. In spite of the fact that such a low atmospheric nitrogen deposition rate would be enough to induce nitrogen limitation in unproductive mountain lakes, phosphorus deposition was also extremely low, and the resulting lake water N: P ratio was unaffected by atmospheric nutrient deposition. In the end, the studied lakes' phytoplankton appeared to be split between phosphorus and nitrogen limitation. We conclude that these pristine lakes are fragile sensitive systems exposed to the predicted climate warming, increased winter precipitation, enhanced forest fires and shifts in anthropogenic nitrogen emissions that could finally couple their water chemistry to that of atmospheric nutrient deposition and unlock temperature-inhibited responses of phytoplankton to nutrient shifts. © Author(s) 2021. This work is distributed under the Creative Commons Attribution 4.0 License.
Scopus
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10.

Вид документа : Статья из сборника (выпуск монографической серии)
Шифр издания :
Автор(ы) : Gros J.-B., Lasseur C., Tikhomirov A.A., Manukovsky N.S., Ushakova S.A., Zolotukhin I.G., Tirranen L.S., Borodina E.V., Kovalev V.S.
Заглавие : Wheat growth on neutral and soil-like substrates: Carbon dioxide exchange and microflora
Место публикации : Acta Horticulturae. - 2004. - Vol. 644. - С. 243-248
Ключевые слова (''Своб.индексиров.''): carbon dioxide--closed ecological system
Аннотация: The soil-like substrate (SLS), which is a potential candidate for employment in closed ecological systems, has been tested. CO2 exchange, composition and numbers of soil microflora have been examined in .wheat-SLS. system. The results produced have been compared to analogous characteristics of .wheat-neutral substrate. system. A hydroponic method was used under wheat growing on the neutral substrate (expanded clay aggregate). Plants for both studies were grown in closed environment from seed to physiological maturity. In the .wheat-SLS. system, the net photosynthetic rate of canopy was positive in the course of 6.55 days after planting. The net photosynthetic rate of canopy in .wheat-neutral substrate. system was positive in the entire course of vegetation. According to calculations in the course of vegetation, photosynthesis has withdrawn 3.28 kg m-2 CO2 from the .wheat-SLS. system and 3.40 kg m-2 CO2 from the .wheat-neutral substrate. system. On the SLS dominant among bacteria were the spore-forming bacteria from Bacillus genus, among fungi . from Trichoderma genus. In the hydroponic cultivation on neutral substrate dominant were bacteria from Pseudomonas genus, most commonly found fungi were species from Fusarium and Botrytis genera.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik, Nadezhda N., Yurchenko, Yuri A., Belevich, Olga E., Kalachova, Galina S., Kolmakova, Anzhelika A., Gladyshev, Michail I.
Заглавие : Waterbugs (Heteroptera: Nepomorpha and Gerromorpha) as sources of essential n-3 polyunsaturated fatty acids in Central Siberian ecoregions
Колич.характеристики :15 с
Коллективы : Russian Foundation for Basic Research [13-04-00860]; Russian Federal Tasks of Fundamental Research [51.1.1, VI.51.1.9]; Russian Federation [NSh-9249.2016.5]
Место публикации : Freshw. Biol.: WILEY-BLACKWELL, 2016. - Vol. 61, Is. 10. - С. 1787-1801. - ISSN 0046-5070, DOI 10.1111/fwb.12818. - ISSN 1365-2427(eISSN)
Примечания : Cited References:77. - The work was supported by award no. 13-04-00860 from the Russian Foundation for Basic Research and by the Russian Federal Tasks of Fundamental Research (projects no. 51.1.1 and VI.51.1.9). The research was partially supported by grant NSh-9249.2016.5 from the President of the Russian Federation.
Предметные рубрики: FRESH-WATER
TERRESTRIAL ECOSYSTEMS
BUGS HETEROPTERA
AQUATIC INSECTS
Ключевые слова (''Своб.индексиров.''): essential fatty acids--heteroptera--subsidies--terrestrial consumers--waterbugs--water-land transfers
Аннотация: 1. Aquatic systems are considered to be a main source of essential long-chain n-3 polyunsaturated fatty acids (PUFA), which are preferentially synthesized by microalgae and transferred along food chains to terrestrial consumers. Emerging aquatic insects comprise a significant part of this transfer of the essential PUFA from water to land. Quantitative data on PUFA content and composition are available mainly for rheophilic insects while taxa that are characteristic of wetlands and stagnant water bodies, such as aquatic Heteroptera, remain relatively unstudied. 2. We investigated the role of various waterbug taxa (Heteroptera: Nepomorpha and Gerromorpha) inhabiting different ecoregions in temperate Central Siberia (Russia) as potential sources of PUFA. The ecoregions were steppe, forest-steppe, hemiboreal forest and montane coniferous forest. Although these waterbugs insects are aquatic in both larval and adult stages, they can disperse through the landscape and transfer essential PUFAs from water to land so making them potentially available to terrestrial consumers. 3. Species of Naucoridae, Notonectidae and Corixidae were generally dominant in all ecoregions, attaining maximum biomass in the steppe. We showed that habitat or ecoregion played a major role in the variability of fatty acid composition of Notonectidae and Gerridae but not Corixidae. In contrast, the biochemical composition of the only naucoridae, Ilyocoris cimicoides, was largely affected by life stage. 4. Both the dominant families and species within them differed with respect to their mass-specific contents of essential long-chain PUFA of the n-3 family, namely eicosapentaenoic and docosahexaenoic acids. Corixid species had the highest content of these two essential PUFAs among the waterbug studies, and relative to literature reports for other aquatic insects. Corixids thus appear to be a potentially important vector for transfer of the essential biochemical compounds from water to land, especially in steppe ecoregions with numerous ephemeral water bodies.
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12.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Manukovsky N.S., Kovalev V.S., Rygalov V.Y., Zolotukhin I.G.
Заглавие : Waste bioregeneration in life support CES: Development of soil organic substrate
Колич.характеристики :6 с
Место публикации : LIFE SCIENCES: LIFE SUPPORT SYSTEMS STUDIES-I. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON PRESS LTD, 1997. - Vol. 20: F4.6, F4.8, F4.2 and F4.9 Symposia of COSPAR Scientific Commission F on Life Sciences - Life Support System Studies-I, at the 31st COSPAR Scientific Assembly (JUL 14-SEP 21, 1996, BIRMINGHAM, ENGLAND), Is. 10. - P1827-1832. - ISBN 0273-1177, DOI 10.1016/S0273-1177(97)00848-X. - ISBN 0-08-043307-3
Примечания : Cited References: 13
Аннотация: An experimental model of matter turnover in the biotic cycle: plants (plant biomass) -- mushrooms (residual substrate + mushroom fruit bodies) -- worms (biohumus) -- microorganisms (soil-like substrate) -- plants is presented. The initial mass of soillike substrate was produced from wheat plants grown in a hydroponic system, Three cycles of matter turnover in the biotic cycle were carried out. Grain productivity on soillike substrate was 21.87 g/m(2) day(1). The results obtained were used for designing a CES containing man, plants, soillike substrate, bioregeneration module and aquaculture. It was shown, that the circulating dry mass of the CES is 756 kg. The main part (88%) of the circulating mass accumulates in the soillike substrate and bioregeneration module. (C) 1997 COSPAR. Published by Elsevier Science Ltd.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gitelson I.I., Tikhomirov A.A., Parshina O.V., Ushakova S.A., Kalacheva G.S.
Заглавие : Volatile metabolites of higher plant crops as a photosynthesizing life support system component under temperature stress at different light intensities
Место публикации : Advances in Space Research. - 2003. - Vol. 31, Is. 7. - С. 1781-1786. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(03)00121-2
Ключевые слова (''Своб.индексиров.''): composition--crops--heat resistance--metabolites--photosynthesis--volatile metabolites--space research--biosphere--article--comparative study--gas--growth, development and aging--heat--indoor air pollution--light--metabolism--microclimate--photon--photosynthesis--physiology--radiation exposure--volatilization--wheat--air pollution, indoor--environment, controlled--gases--heat--life support systems--light--photons--photosynthesis--triticum--volatilization
Аннотация: The effect of elevated temperatures of 35 and 45В°C (at the intensities of photosynthetically active radiation 322, 690 and 1104 ?mol-m-2-s-1) on the photosynthesis, respiration, and qualitative and quantitative composition of the volatiles emitted by wheat (Triticum aestuvi L., cultivar 232) crops was investigated in growth chambers. Identification and quantification of more than 20 volatile compounds (terpenoids - ?-pinene, ?3 carene, limonene, benzene, ?-and transcaryophyllene, ?- and ?-terpinene, their derivatives, aromatic hydrocarbons, etc.) were conducted by gas chromatograph/mass spectrometry. Under light intensity of 1104 ?mol-m-2-s-1, heat resistance of photosynthesis and respiration increased at 35В°C and decreased at 45В°C. The action of elevated temperatures brought about variations in the rate and direction of the synthesis of volatile metabolites. The emission of volatile compounds was the greatest under a reduced irradiation of 322 ?mol-m-2-s-1 and the smallest under 1104 ?mol-m-2-s-1 at 35В°C. During the repair period, the contents and proportions of volatile compounds were different from their initial values, too. The degree of disruption and the following recovery of the functional state depended on the light intensity during the exposure to elevated temperatures. The investigation of the atmosphere of the growth chamber without plants has revaled the substances that were definitely technogenic in origin: tetramethylurea, dimethylsulfide, dibutylsulfide, dibutylphthalate, and a number of components of furan and silane nature. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Alieva R. R., Kudryasheva N. S.
Заглавие : Variability of fluorescence spectra of coelenteramide-containing proteins as a basis for toxicity monitoring
Место публикации : Talanta: Elsevier B.V., 2017. - Vol. 170. - С. 425-431. - ISSN 00399140 (ISSN) , DOI 10.1016/j.talanta.2017.04.043
Ключевые слова (''Своб.индексиров.''): coelenteramide-containing fluorescent protein--multicolor fluorescent bioassay--obelin--primary photochemical process--protein destruction--proton transfer--bioassay--biomarkers--excited states--fluorescence--fluorophores--ionizing radiation--proton transfer--toxicity--electron-excited state--fluorescence spectra--fluorescent protein--green fluorescent protein--obelin--photochemical process--photochemical properties--physicochemical process--proteins
Аннотация: Nowadays, physicochemical approach to understanding toxic effects remains underdeveloped. A proper development of such mode would be concerned with simplest bioassay systems. Coelenteramide-Containing Fluorescent Proteins (CLM-CFPs) can serve as proper tools for study primary physicochemical processes in organisms under external exposures. CLM-CFPs are products of bioluminescent reactions of marine coelenterates. As opposed to Green Fluorescent Proteins, the CLM-CFPs are not widely applied in biomedical research, and their potential as colored biomarkers is undervalued now. Coelenteramide, fluorophore of CLM-CFPs, is a photochemically active molecule; it acts as a proton donor in its electron-excited states, generating several forms of different fluorescent state energy and, hence, different fluorescence color, from violet to green. Contributions of the forms to the visible fluorescence depend on the coelenteramide microenvironment in proteins. Hence, CLM-CFPs can serve as fluorescence biomarkers with color differentiation to monitor results of destructive biomolecule exposures. The paper reviews experimental and theoretical studies of spectral-luminescent and photochemical properties of CLM-CFPs, as well as their variation under different exposures – chemicals, temperature, and ionizing radiation. Application of CLM-CFPs as toxicity bioassays of a new type is justified. © 2017
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gaevsky N.A., Kolmakov V.I., Anishchenko O.V., Gorbaneva T.B.
Заглавие : Using DCMU-fluorescence method for the identification of dominant phytoplankton groups
Колич.характеристики :12 с
Место публикации : J. Appl. Phycol.: SPRINGER, 2005. - Vol. 17, Is. 6. - P483-494. - ISSN 0921-8971, DOI 10.1007/s10811-005-2903-x
Примечания : Cited References: 28
Предметные рубрики: IN-VIVO
CHLOROPHYLL FLUORESCENCE
FLUOROMETRIC METHOD
SITU
PHOTOSYNTHESIS
CYANOBACTERIA
ALGAE
EVOLUTION
CULTURES
LAKES
Ключевые слова (''Своб.индексиров.''): algae--chlorophyll--fluorescence--vizualization
Аннотация: For the identification of ecologically significant dominant groups of phytoplanktonic algae a polychromatic DCMU-induced fluorescence method is recommended. A special fluorometer equipped with a system of replaceable filters is used to differentiate three regions of the spectrum (410 +/- 20, 510 +/- 20 and 540 +/- 10 nm) that can excite the basic light-harvesting pigments. Total and differential (for every algal taxon studied) chlorophyll a calculated from the fluorescence signals is in good agreement with biomass estimates from direct cell counts for several different trophic types of aquatic systems. This is made possible by the vizualization of the ratios of fluorescence signal values in their own coordinates: first, to decide whether it is necessary to correct linear equations in order to eliminate negative solutions; second, to determine the possibility of nulling the negative solution if a point is situated close to a side of the triangle; and third, to reduce the number of linear algebraic equations to two if the points are situated along one of the triangle sides or to one if the points are gathered at the apex. The polychromatic DCMU-induced fluorescence method can be used for monitoring natural phytoplankton populations to detect changes in their taxonomic structure.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tikhomirov A.A., Kudenko Y.A., Ushakova S.A., Tirranen L.S., Gribovskaya I.A., Gros J.-B., Lasseur C.
Заглавие : Use of human wastes oxidized to different degrees in cultivation of higher plants on the soil-like substrate intended for closed ecosystems
Место публикации : Advances in Space Research. - 2010. - Vol. 46, Is. 6. - С. 744-750. - ISSN 02731177 (ISSN) , DOI 10.1016/j.asr.2010.02.024
Ключевые слова (''Своб.индексиров.''): life support systems--microflora--mineralized human wastes--phototrophic unit--wet incineration--alternating electromagnetic field--bioregenerative life support systems--degree of oxidations--denitrifying microorganisms--growth and development--higher plants--human waste--life support systems--mass exchange--microbiotas--microflora--microscopic fungi--mineralized human wastes--nutrient solution--oxidation level--phytopathogenic bacteria--plant productivity--soil-like substrate--wheat plants--biomolecules--electromagnetic fields--irrigation--magnetic field effects--metabolism--metabolites--oxidation--plants (botany)--soils--solvent extraction--wastes--waste incineration
Аннотация: To close mass exchange loops in bioregenerative life support systems more efficiently, researchers of the Institute of Biophysics SB RAS (Krasnoyarsk, Russia) have developed a procedure of wet combustion of human wastes and inedible parts of plants using H2O2 in alternating electromagnetic field. Human wastes pretreated in this way can be used as nutrient solutions to grow plants in the phototrophic unit of the LSS. The purpose of this study was to explore the possibilities of using human wastes oxidized to different degrees to grow plants cultivated on the soil-like substrate (SLS). The treated human wastes were analyzed to test their sterility. Then we investigated the effects produced by human wastes oxidized to different degrees on growth and development of wheat plants and on the composition of microflora in the SLS. The irrigation solution contained water, substances extracted from the substrate, and certain amounts of the mineralized human wastes. The experiments showed that the human wastes oxidized using reduced amounts of 30% H2O2: 1 ml/g of feces and 0.25 ml/ml of urine were still sterile. The experiments with wheat plants grown on the SLS and irrigated by the solution containing treated human wastes in the amount simulating 1/6 of the daily diet of a human showed that the degree of oxidation of human wastes did not significantly affect plant productivity. On the other hand, the composition of the microbiota of irrigation solutions was affected by the oxidation level of the added metabolites. In the solutions supplemented with partially oxidized metabolites yeast-like microscopic fungi were 20 times more abundant than in the solutions containing fully oxidized metabolites. Moreover, in the solutions containing incompletely oxidized human wastes the amounts of phytopathogenic bacteria and denitrifying microorganisms were larger. Thus, insufficiently oxidized sterile human wastes added to the irrigation solutions significantly affect the composition of the microbiological component of these solutions, which can ultimately unbalance the system as a whole. В© 2010 COSPAR. Published by Elsevier Ltd. All rights reserved.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Balnokin Y., Nikolai M., Popova L., Tikhomirov A., Ushakova S., Lasseur C., Gros J.-B.
Заглавие : Use of halophytic plants for recycling NaCl in human liquid waste in a bioregenerative life support system
Место публикации : Advances in Space Research. - 2010. - Vol. 46, Is. 6. - С. 768-774. - ISSN 02731177 (ISSN) , DOI 10.1016/j.asr.2010.03.020
Ключевые слова (''Своб.индексиров.''): biological life support system--halophytes--human waste recycling--nacl recycling--a plants--biological life support systems--biomass productions--bioregenerative life support systems--daily ration--europaea--growth conditions--halophytes--halophytic plants--human waste--liquid wastes--nutrient solution--optimal conditions--salicornia europaea--body fluids--liquids--nutrients--plant shutdowns--plants (botany)--recycling--sodium alloys--sodium chloride
Аннотация: The purpose of this work was to develop technology for recycling NaCl containing in human liquid waste as intrasystem matter in a bioregenerative life support system (BLSS). The circulation of Na+ and Cl- excreted in urine is achieved by inclusion of halophytes, i.e. plants that naturally inhabit salt-rich soils and accumulate NaCl in their organs. A model of Na+ and Cl- recycling in a BLSS was designed, based on the NaCl turnover in the human-urine-nutrient solution-halophytic plant-human cycle. The study consisted of (i) selecting a halophyte suitable for inclusion in a BLSS, and (ii) determining growth conditions supporting maximal Na + and Cl- accumulation in the shoots of the halophyte growing in a nutrient solution simulating mineralized urine. For the selected halophytic plant, Salicornia europaea, growth rate under optimal conditions, biomass production and quantities of Na+ and Cl- absorbed were determined. Characteristics of a plant production conveyor consisting of S. europaea at various ages, and allowing continuity of Na+ and Cl - turnover, were estimated. It was shown that closure of the NaCl cycle in a BLSS can be attained if the daily ration of fresh Salicornia biomass for a BLSS inhabitant is approximately 360 g. В© 2010 COSPAR. Published by Elsevier Ltd. All rights reserved.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kudryasheva N.S., Nemtseva E.V., Meshalkin Y.P., Sizykh A.G.
Заглавие : Upper electron-excited states in bioluminescence: experimental indication
Колич.характеристики :4 с
Место публикации : Luminescence: JOHN WILEY & SONS LTD, 2001. - Vol. 16, Is. 3. - P243-246. - ISSN 1522-7235, DOI 10.1002/bio.613
Примечания : Cited References: 22
Ключевые слова (''Своб.индексиров.''): bioluminescence--upper electron-excited states--energy transfer
Аннотация: The involvement of upper electron-excited states in bacterial bioluminescence process was studied with excitation energy-accepting molecules. The fluorescent aromatic compounds, anthracene and 1.4-bis(5-phenyloxazol-2-yl)benzene, were chosen. Energies of their lowest excited singlet states are higher than the energy of the analogous state of the bioluminescence emitter; their absorption spectra and bioluminescence do not overlap. Hence, the excitation of these molecules by singlet-singlet energy transfer or by light absorption is excluded. Sensitized fluorescence of these compounds in the bioluminescence systems has been recorded, indicating the activity of upper electron-excited states in the bioluminescent process. Copyright (C) 2001 John Wiley & Sons, Ltd.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Van Stokkum I.H.M., Gobets B., Van Mourik F., Lee J., Van Grondelle R., Visser A.J.W.G.
Заглавие : Ultrafast fluorescence relaxation spectroscopy of 6,7-dimethyl-(8-ribityl)-lumazine and riboflavin, free and bound to antenna proteins from bioluminescent bacteria
Место публикации : Journal of Physical Chemistry B. - 2003. - Vol. 107, Is. 39. - С. 10934-10939. - ISSN 15206106 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacteria--bioluminescence--chemical relaxation--chromophores--dielectric properties--proteins--solvents--bioluminescent bacteria--dimethyl ribityl lumazine--photobacterium leiognathi--riboflavin--ultrafast fluorescence relaxation spectroscopy--fluorescence
Аннотация: The solvation dynamics of interesting bioluminescent chromophores have been determined, using subpicosecond and wavelength-resolved fluorescence spectroscopy, in combination with global analysis of the multidimensional data sets. The systems investigated comprise the free ligands 6,7-dimethyl-(8-ribityl)-lumazine (lumazine) and riboflavin in an aqueous buffer and both ligands when noncovalently bound to two bacterial bioluminescent antenna proteins: lumazine protein (from Photobacterium leiognathi) and the blue fluorescent protein (from Vibrio fischeri Y1). Fluorescence spectral relaxation of the free ligands is complete within a few picoseconds. Subsequently, the fluorescence intensity increases by ?7% on a time scale of 15-30 ps. Fluorescence spectral relaxation of the protein-bound ligands is largely complete within 1 ps but reveals a small red shift with a minor, but distinctly longer, relaxation time than that of the free ligands, which is tentatively assigned to the relaxation of protein-bound water in the vicinity of the excited chromophore.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., van Stokkum IHM, Gobets B..., van Mourik F..., Lee J..., van Grondelle R..., Visser AJWG
Заглавие : Ultrafast fluorescence relaxation spectroscopy of 6,7-dimethyl-(8-ribityl)-lumazine and riboflavin, free and bound to antenna proteins from bioluminescent bacteria
Колич.характеристики :6 с
Место публикации : J. Phys. Chem. B: AMER CHEMICAL SOC, 2003. - Vol. 107, Is. 39. - P10934-10939. - ISSN 1520-6106, DOI 10.1021/jp034266e
Примечания : Cited References: 52
Предметные рубрики: TIME-RESOLVED FLUORESCENCE
VIBRIO-FISCHERI Y1
FEMTOSECOND SOLVATION DYNAMICS
FLAVIN ADENINE-DINUCLEOTIDE
PHOTOBACTERIUM-LEIOGNATHI
BIOLOGICAL WATER
SOLVENT DYNAMICS
DIELECTRIC-RELAXATION
MOLECULAR-DYNAMICS
TRYPTOPHAN
Аннотация: The solvation dynamics of interesting bioluminescent chromophores have been determined, using subpicosecond and wavelength-resolved fluorescence spectroscopy, in combination with global analysis of the multidimensional data sets. The systems investigated comprise the free ligands 6,7-dimethyl-(8-ribityl)-lumazine (lumazine) and riboflavin in an aqueous buffer and both ligands when noncovalently bound to two bacterial bioluminescent antenna proteins: lumazine protein (from Photobacterium leiognathi) and the blue fluorescent protein (from Vibrio fischeri Y1). Fluorescence spectral relaxation of the free ligands is complete within a few picoseconds. Subsequently, the fluorescence intensity increases by similar to7% on a time scale of 15-30 ps. Fluorescence spectral relaxation of the protein-bound ligands is largely complete within 1 ps but reveals a small red shift with a minor, but distinctly longer, relaxation time than that of the free ligands, which is tentatively assigned to the relaxation of protein-bound water in the vicinity of the excited chromophore.
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