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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Esimbekova E. N., Asanova A. A., Deeva A. A., Kratasyuk V. A.
Заглавие : Inhibition effect of food preservatives on endoproteinases
Место публикации : Food Chem.: Elsevier Ltd, 2017. - Vol. 235. - С. 294-297. - ISSN 03088146 (ISSN) , DOI 10.1016/j.foodchem.2017.05.059
Ключевые слова (''Своб.индексиров.''): endoproteinases--food additives--pancreatic disease--pancreatic enzymes--benzoic acid--enzyme activity--enzymes--food additives--food preservatives--potassium sorbate--sodium--acceptable daily intakes--decay constants--endoproteinases--human metabolisms--inhibition effect--light intensity--protein digestion--sodium benzoate--sorbic acid
Аннотация: The present manuscript proposes a novel approach to assess the impact of food additives on human metabolism by analysing their effect on biomarker enzyme activity. Alterations in the activity of pancreatic enzymes, such as chymotrypsin and trypsin, which are affected by the most common food preservatives, sodium benzoate (E211), potassium sorbate (E202) and sorbic acid (E200), have been evaluated. The proteinase activity was analysed with a bioluminescent method using the light intensity decay constant. Our study revealed that the preservatives reduce proteinase activity by 50% (EC50) at a much lower concentration than their acceptable daily intake (ADI). Thus, sodium benzoate and sorbic acid have an inhibition effect on chymotrypsin at concentrations 14 times lower and 70 times lower than their ADI and this increases with exposure time. Food preservative consumption impacts negatively on protein digestion, which is especially dangerous for patients with pancreatitis. © 2017 Elsevier Ltd
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Eremeeva E. V., Vysotski E. S.
Заглавие : Bioluminescent and biochemical properties of Cys-free Ca2+-regulated photoproteins obelin and aequorin
Место публикации : J. Photochem. Photobiol. B Biol.: Elsevier B.V., 2017. - Vol. 174. - С. 97-105. - ISSN 10111344 (ISSN) , DOI 10.1016/j.jphotobiol.2017.07.021
Ключевые слова (''Своб.индексиров.''): bioluminescence--coelenteramide--coelenterazine--cysteine--photoprotein--serine
Аннотация: Bioluminescence of a variety of marine coelenterates is determined by Ca2+-regulated photoproteins. A strong interest in these proteins is for their wide analytical potential as intracellular calcium indicators and labels for in vitro binding assays. The presently known hydromedusan Ca2+-regulated photoproteins contain three (aequorin and clytin) or five (obelin and mitrocomin) cysteine residues with one of them strictly conserved. We have constructed Cys-free aequorin and obelin by substitution of all cysteines to serine residues. Such mutants should be of interest for researchers by the possibility to avoid the incubation with dithiothreitol (or ?-mercaptoethanol) required for producing an active photoprotein that is important for some prospective analytical assays in which the photoprotein is genetically fused with a target protein sensitive to the reducing agents. Cys-free mutants were expressed in Escherichia coli, purified, and characterized regarding the efficiency of photoprotein complex formation, functional activity, and conformational stability. The replacement of cysteine residues has been demonstrated to affect different properties of aequorin and obelin. Cys-free aequorin displays a two-fold lower specific bioluminescence activity but preserves similar activation properties and light emission kinetics compared to the wild-type aequorin. In contrast, Cys-free obelin retains only ~ 10% of the bioluminescence activity of wild-type obelin as well as binding coelenterazine and forming active photoprotein much less effectively. In addition, the substitution of Cys residues drastically changes the bioluminescence kinetics of obelin completely eliminating a “fast” component from the light signal decay curve. At the same time, the replacement of Cys residues increases conformational flexibility of both aequorin and obelin molecules, but again, the effect is more prominent in the case of obelin. The values of thermal midpoints of unfolding (Tm) were determined to be 53.3 ± 0.2 and 44.6 ± 0.4 °C for aequorin and Cys-free aequorin, and 49.1 ± 0.1 and 28.8 ± 0.3 °C for obelin and Cys-free obelin, respectively. Thus, so far only Cys-free aequorin is suitable as a partner for fusing with a tag sensitive to reducing agents since the aequorin mutant preserves almost 50% of the bioluminescent activity and can be produced with a substantial yield. © 2017 Elsevier B.V.
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Вид документа : Статья из сборника (выпуск монографической серии)
Шифр издания :
Автор(ы) : Bartsev S., Belolipetskii P., Degermendzhi A.
Заглавие : Multistable states in the biosphere-climate system: towards conceptual models
Колич.характеристики :10 с
Коллективы : RFBR-KKFN [15-41-04300]; Complex Program of SB RAS [II.2, 0360-2015-0002]
Место публикации : V INTERNATIONAL WORKSHOP ON MATHEMATICAL MODELS AND THEIR APPLICATIONS: IOP PUBLISHING LTD, 2017. - Vol. 173: 5th International Workshop on Mathematical Models and their Applications (NOV 07-09, 2016, Krasnoyarsk, RUSSIA). - Ст.UNSP 012005. - (IOP Conference Series-Materials Science and Engineering). - , DOI 10.1088/1757-899X/173/1/012005
Примечания : Cited References:31. - This work was supported by grant RFBR-KKFN No 15-41-04300 and Complex Program of SB RAS No II.2. No 0360-2015-0002.
Предметные рубрики: SEA-SURFACE TEMPERATURE
1980S REGIME SHIFT
EL-NINO
PACIFIC
Аннотация: Forecasting response of the biosphere and regional ecosystems to observed and expected climate change is the fundamental problem with obvious practical significance. Fundamental non-linearity of the climate system and biosphere makes feasible implementing multiple states and threshold processes in the biosphere-climate system (BCS) in response to gradually increasing influence factor (greenhouse gas concentrations growth). Really time series analysis of global temperature and other global and local parameters indicates the presence of abrupt transitions between stationary states. Identification of the switching mechanisms using general circulation models of the atmosphere and the ocean is associated with the obvious difficulties due to their complexity. Understanding the nature of such switches at qualitative level can be achieved by using a conceptual small-scale models. Some variants of possible mechanisms capable of generating these shifts and simultaneously supporting quasi-stationary periods between them are discussed.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prudnikova S. V., Vinogradova O. N., Trusova M. Y.
Заглавие : Specific character of bacterial biodegradation of polyhydroxyalkanoates with different chemical structure in soil
Место публикации : Doklad. Biochem. Biophys.: Maik Nauka Publishing / Springer SBM, 2017. - Vol. 473, Is. 1. - С. 94-97. - ISSN 16076729 (ISSN) , DOI 10.1134/S1607672917010185
Аннотация: The study addresses the influence of the physicochemical properties of the reserve cellular macromolecules (polyhydroxyalkanoates, PHA) with different chemical composition on their biodegradation in the agro-transformed field soil of the Siberian region (Krasnoyarsk Territory, Russia). It was shown that the degradation of the PHA samples depends on the degree of polymer crystallinity (Cx). For the first time, it was shown that the range of PHA-degrading microorganisms differs for each of PHA types. The study defines the primary degraders specific to each PHA type and common to all types of examined polymers. © 2017, Pleiades Publishing, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bolsunovsky, Alexander, Melgunov, Mikhail, Chuguevskii, Alexey, Lind, Ole Christian, Salbu, Brit
Заглавие : Unique diversity of radioactive particles found in the Yenisei River floodplain
Колич.характеристики :10 с
Коллективы : Research Council of Norway through its Centres of Excellence funding scheme [223268/F50]; IAEA [17941]
Место публикации : Sci Rep: NATURE PUBLISHING GROUP, 2017. - Vol. 7. - Ст.11132. - ISSN 2045-2322, DOI 10.1038/s41598-017-11557-7
Примечания : Cited References:22. - The authors are grateful to researchers of the Institute of Biophysics FRC KSC SB RAS (Krasnoyarsk, Russia) and the Institute of Geology and Mineralogy SB RAS (Novosibirsk, Russia) for their assistance in isolation and analysis of radioactive particles. Part of the work is also supported by the Research Council of Norway through its Centres of Excellence funding scheme, project number 223268/F50. Investigations of the particles were partially supported by IAEA (Project 17941).
Предметные рубрики: CONTAINING CONTROL RODS
TRANSURANIC ELEMENTS
BOTTOM SEDIMENTS
HOT
Аннотация: The long-term operation of three reactors and the radiochemical plant of the Mining-and-Chemical Combine (MCC), Russia's largest producer of weapons-grade plutonium, has resulted in radioactive contamination of the Yenisei River floodplain. From 1995 to 2016, we found more than 200 radioactive particles (RP) in the Yenisei floodplain, downstream of the MCC. Analytical characterization showed that most of the RP were fuel particles, which were carried into the river after incidents at the MCC reactors. Having compared the Cs-137/Cs-134 ratios in the particles, we determined three time intervals when the RP were formed. The plutonium isotope ratios (Pu-238/Pu-239,Pu-240) vary substantially between the particles and indicate several different source terms. In addition to fuel RP, we found particles that only contained activation products (Co-60 or europium isotopes). SEM and gamma-spectrometry showed that the cobalt particles could have originated from the corrosion of the reactor coolant system and the europium particles -from the damaged compensating rods. No europium particles have been found anywhere else in the world. The presence of RP from different sources (fuel, cobalt, and europium particles) in the Yenisei River floodplain makes this region a unique site for studying environmental effects of the particles. These RP represent point sources of radioecological significance.
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Вид документа :
Шифр издания :
Автор(ы) : Volova T. G., Goncharov D. B., Nikolaeva E. D., Shishatskaya E. I.
Заглавие : Electrospinning of degradable phas: Process, properties, applications
Место публикации :: Nova Science Publishers, Inc., 2017. - С. 1-56
Ключевые слова (''Своб.индексиров.''): biological and physical/mechanical properties--cell proliferation--electrospinning--nonwoven membranes--pha--scaffolds--skin regeneration--ultrafine fibers--wound dressings
Аннотация: An integrated study has been performed to investigate the process of formation of ultrafine fibers and nonwoven membranes by electrospinning from natural degradable polymers-polyhydroxyalkanoates (PHAs); physical, mechanical, and biological properties of the products have been studied. Then, electrospinning was used to prepare ultrafine fibers from PHAs with different compositions: P(3HB) and its copolymers P(3HB-co-4HB), P(3HB-co-3HV), and P(3HB-co-3HHx). The main process parameters, that influence UF-fiber diameter and properties of fibrous non-woven membranes) (polymer concentration, solution feeding rate, working distance, and applied voltage), were investigated and their effects evaluated. This study was the first to compare biological and physical/mechanical parameters of PHAs with different chemical compositions as dependent upon the fractions of monomers, constituting the polymers and fiber orientation. Electrospun polymer membranes, prepared from the [P(3HB-co-4HB)], were tested as wound dressings. The developed nonwoven membranes can be used as the equivalent of collagen skine dressings in the treatment of burns of degree II. Experiments on laboratory animals with model skin defects showed, that the membranes fitted the wound shape good, protected the wound from external influences, and facilitated wound healing, promoting fast repair. The successful experiments on laboratory animals were followed by pilot clinical trials of nanomembranes, comprised of PHA membranes as wound dressings in the treatment of septic wounds. During the regeneration phase, PHA membranes served as a scaffold for the new tissue on the skin and filled out soft tissue defects. The formation of the uniform and sufficiently vascularized tissue is a prerequisite for quicker wound healing and can serve as a basis for the subsequent skin grafting and spontaneous re-epithelialization of superficial wounds. The wound dressing, tested in this clinical trial, performs important physiological functions of natural skin, provides a barrier against secondary infection, reduces fluid loss, and, at the same time, does not keep the air out. © 2017 Nova Science Publishers, Inc.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nemtseva, Elena V., Lashchuk, Olesya O., Gerasimova, Marina A., Melnik, Tatiana N., Nagibina, Galina S., Melnik, Bogdan S.
Заглавие : Fluorescence lifetime components reveal kinetic intermediate states upon equilibrium denaturation of carbonic anhydrase II
Колич.характеристики :9 с
Коллективы : Ministry for Science and Education of the Russian Federation [6.7734.2017/BCH]; Russian Science Foundation [N14-24-00157]
Место публикации : Methods Appl. Fluoresc.: IOP PUBLISHING LTD, 2018. - Vol. 6, Is. 1. - Ст.015006. - ISSN 2050-6120, DOI 10.1088/2050-6120/aa994a
Примечания : Cited References:28. - The study of time-resolved protein fluorescence was supported by the Ministry for Science and Education of the Russian Federation (project 6.7734.2017/BCH). Kinetic and genetic engineering studies of carbonic anhydrase II were supported by grant N14-24-00157 from the Russian Science Foundation.
Предметные рубрики: PROTEIN FLUORESCENCE
TRYPTOPHAN PROTEINS
RESIDUES
STABILITY
Ключевые слова (''Своб.индексиров.''): time-resolved fluorescence spectroscopy--carbonic anhydrase ii--protein--intermediate states--comparison of kinetic and equilibrium experiments--protein fluorescence lifetime
Аннотация: In most cases, intermediate states of multistage folding proteins are not 'visible' under equilibrium conditions but are revealed in kinetic experiments. Time-resolved fluorescence spectroscopy was used in equilibrium denaturation studies. The technique allows for detecting changes in the conformation and environment of tryptophan residues in different structural elements of carbonic anhydrase II which in its turn has made it possible to study the intermediate states of carbonic anhydrase II under equilibrium conditions. The results of equilibrium and kinetic experiments using wild-type bovine carbonic anhydrase II and its mutant form with the substitution of leucine for alanine at position 139 (L139A) were compared. The obtained lifetime components of intrinsic tryptophan fluorescence allowed for revealing that, the same as in kinetic experiments, under equilibrium conditions the unfolding of carbonic anhydrase II ensues through formation of intermediate states.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Eremeeva, Elena V., Vysotski, Eugene S.
Заглавие : Bioluminescent and biochemical properties of Cys-free Ca2+-regulated photoproteins obelin and aequorin
Колич.характеристики :9 с
Коллективы : Russian Academy of Sciences [03562016-0712, 0356-2015-0103]; RFBR [17-04-00764]
Место публикации : J. Photochem. Photobiol. B-Biol.: ELSEVIER SCIENCE SA, 2017. - Vol. 174. - С. 97-105. - ISSN 1011-1344, DOI 10.1016/j.jphotobio1.2017.07.021
Примечания : Cited References:54. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences (projects 03562016-0712 and 0356-2015-0103) and the RFBR grant 17-04-00764.
Предметные рубрики: SEQUENCE-ANALYSIS
APO-OBELIN
INTRINSIC FLUORESCENCE
COELENTERAZINE
Ключевые слова (''Своб.индексиров.''): bioluminescence--coelenterazine--photoprotein--coelenteramide--cysteine--serine
Аннотация: Bioluminescence of a variety of marine coelenterates is determined by Ca2+-regulated photoproteins. A strong interest in these proteins is for their wide analytical potential as intracellular calcium indicators and labels for in vitro binding assays. The presently known hydromedusan Ca2+-regulated photoproteins contain three (aequorin and clytin) or five (obelin and mitrocomin) cysteine residues with one of them strictly conserved. We have constructed Cys-free aequorin and obelin by substitution of all cysteines to serine residues. Such mutants should be of interest for researchers by the possibility to avoid the incubation with dithiothreitol (or p-mercaptoethanol) required for producing an active photoprotein that is important for some prospective analytical assays in which the photoprotein is genetically fused with a target protein sensitive to the reducing agents. Cys-free mutants were expressed in Escherichia coil, purified, and characterized regarding the efficiency of photoprotein complex formation, functional activity, and conformational stability. The replacement of cysteine residues has been demonstrated to affect different properties of aequorin and obelin. Cys-free aequorin displays a two-fold lower specific bioluminescence activity but preserves similar activation properties and light emission kinetics compared to the wild -type aequorin. In contrast, Cys-free obelin retains only 10% of the bioluminescence activity of wild-type obelin as well as binding coelenterazine and forming active photoprotein much less effectively. In addition, the substitution of Cys residues drastically changes the bioluminescence kinetics of obelin completely eliminating a "fast" component from the light signal decay curve. At the same time, the replacement of Cys residues increases conformational flexibility of both aequorin and obelin molecules, but again, the effect is more prominent in the case of obelin. The values of thermal midpoints of unfolding (Tm) were determined to be 53.3 0.2 and 44.6 0.4 C for aequorin and Cys-free aequorin, and 49.1 0.1 and 28.8 0.3 C for obelin and Cys-free obelin, respectively. Thus, so far only Cys-free aequorin is suitable as a partner for fusing with a tag sensitive to reducing agents since the aequorin mutant preserves almost 50% of the bioluminescent activity and can be produced with a substantial yield.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Rogozin D. Y., Tarnovsky M. O., Belolipetskii V. M., Zykov V. V., Zadereev E. S., Tolomeev A. P., Drobotov A. V., Barkhatov Y. V., Gaevsky N. A., Gorbaneva T. B., Kolmakova A. A., Degermendzhi A. G.
Заглавие : Disturbance of meromixis in saline Lake Shira (Siberia, Russia): Possible reasons and ecosystem response
Колич.характеристики :12 с
Коллективы : Russian Foundation for Basic Research [16-05-00091]; Russian Federation [NSh-9249.2016.5]
Место публикации : Limnologica: ELSEVIER GMBH, URBAN & FISCHER VERLAG, 2017. - Vol. 66. - С. 12-23. - ISSN 0075-9511, DOI 10.1016/j.limno.2017.06.004. - ISSN 1873-5851(eISSN)
Примечания : Cited References:43. - We acknowledge the financial support by the Russian Foundation for Basic Research, grant No 16-05-00091. The research was partially supported by the Council on grants from the President of the Russian Federation for support of leading scientific schools (grant NSh-9249.2016.5). Many thanks to colleagues from analytical laboratory of Institute of Biophysics SB RAS for sulphide determination. We thank the employees of Middle Siberian Department of Russian Hydro Meteorological Service in Krasnoyarsk for providing the data on weather and Lake Shira level. We are grateful to Dr. Martin Schmid and two anonymous reviewers for valuable comments which helped us to improve the article considerably.
Предметные рубрики: SHUNET SOUTH SIBERIA
MEROMICTIC LAKE
VERTICAL STRATIFICATION
WINTER
Ключевые слова (''Своб.индексиров.''): meromixis--mixolimnion--stratification--stability--mixing--food chain--purple sulfur bacteria
Аннотация: Saline Lake Shira (Southern Siberia, Russia) was meromictic through the observation period 2002-2015. During the under-ice periods of 2015 and 2016, complete mixing of the water column was recorded for the first time, and hydrogen sulphide temporarily disappeared from the water column of the lake; i.e. in those years the lake turned to holomixis. In the summer of 2015, a sharp increase in chlorophyll a, organic carbon, zooplankton, and phytoflagellates was observed in the lake, which was probably due to the release of nutrients from the monimolimnion. Purple sulfur bacteria completely disappeared from the lake after the first mixing in 2015, and did not reappear despite the restoration of meromixis in 2017. Thus, it was demonstrated that purple sulfur bacteria are sensitive to the weakening of the stratification of Lake Shira. Based on the data of the seasonal monitoring of temperature and salinity profiles over the period 2002-2017, it was presumed that the main cause of deep mixing in 2015 was the weakening of the salinity gradient due to strong wind impact and early ice retreat in the spring of 2014. In addition, it was shown that in previous years a significant contribution to the maintenance of meromixis was made by an additional influx of fresh water, which caused a rise in the lake level in the period 2002-2007. Thus, we identified a relationship between the stratification regime of the lake and the change in its level, which provides valuable information both for the forecast of Water quality and for reconstruction of the Holocene climate humidity in this region of Southern Siberia from the sediment cores of Lake Shira.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Belousov K. I., Denisov I. A., Lukyanenko K. A., Yakimov A. S., Bukatin A. S., Kukhtevich I. V., Sorokin V. V., Esimbekova E. N., Belobrov P. I., Evstrapov A. A.
Заглавие : Dissolution and mixing of flavin mononucleotide in microfluidic chips for bioassay
Место публикации : J. Phys. Conf. Ser.: Institute of Physics Publishing, 2016. - Vol. 741, Is. 1. - ISSN 17426588 (ISSN) , DOI 10.1088/1742-6596/741/1/012058
Ключевые слова (''Своб.индексиров.''): bioassay--biomolecules--dissolution--flow of fluids--fluidic devices--microfluidics--nanostructures--optoelectronic devices--oscillating flow--photonics--analysis of liquids--concentration distributions--constant flow rates--flavin mono nucleotides (fmn)--flavin mononucleotides--frequency of oscillation--uniform distribution--variable flow rate--mixing
Аннотация: Dissolution and mixing of flavin mononucleotide (FMN), which activates a luminescent reaction, were considered in various designs of microfluidic chip for pollution analysis of liquid samples. The aim was to determine the velocity mode of fluid flow ensured the uniform distribution of the FMN in the reaction chamber. Simulation of concentration distribution of FMN in various designs of microfluidic chips was conducted. It was shown that the passive mixing techniques based on the constant flow rate didn't provide mixing of FMN in acceptable time (3 seconds). The most efficient mixing was achieved using variable flow rate with a gradually increasing frequency of oscillation. © Published under licence by IOP Publishing Ltd.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Barkhatov Y. V., Tikhomirov A. A., Ushakova S. A., Shikhov V. N., Bartsev S. I., Degermendzhi A. G.
Заглавие : Experimental modeling of the influence of the rise in average summer temperatures on carbon circulation in tundra ecosystems
Место публикации : Dokl. Earth Sci.: Maik Nauka-Interperiodica Publishing, 2016. - Vol. 471, Is. 1. - С. 1168-1170. - ISSN 1028334X (ISSN) , DOI 10.1134/S1028334X16110106
Ключевые слова (''Своб.индексиров.''): carbon dioxide--ecology--landforms--carbon fluxes--climate condition--comparative evaluations--experimental modeling--physical simulation--soil respiration--summer temperature--temperature rise--ecosystems
Аннотация: A sealed vegetation chamber was designed and constructed for physical simulation of climate conditions in the Subarctic zone during the spring–summer time. The small laboratory tundra-simulating ecosystem (TSE) was created for comparative evaluation of the rates of soil respiration and of the total balance of carbon fluxes in tundra ecosystems. The test experiment was performed to study the TSE response to a temperature rise in air and soil by 2°C in terms of the intensity of the СО2 flux. It was shown that this increase in temperature would cause a pronounced shift in the balance of СО2 production and utilization in the ecosystem from near-zero values to a stable generation of 24 ?mol/h of CO2 per 1 kg of dry biomass. © 2016, Pleiades Publishing, Ltd.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Rogozin D. Y., Balaev D. A., Semenov S. V., Shaikhutdinov K. A., Bayukov O. A.
Заглавие : Magnetic properties of bottom sediments from Meromectic Shira Lake (Siberia, Russia)
Место публикации : Dokl. Earth Sci.: Maik Nauka-Interperiodica Publishing, 2016. - Vol. 469, Is. 2. - С. 819-823. - ISSN 1028334X (ISSN) , DOI 10.1134/S1028334X16080067
Ключевые слова (''Своб.индексиров.''): magnetic properties--magnetite--sediments--biogenic magnetite--bottom sediments--geochemical characteristic--local minimums--magnetite particles--magnetotactic bacteria--single domains--ssbauer spectroscopies--lakes
Аннотация: Magnetic properties were studied in bottom sediments of saline meromictic Shira Lake by the methods of static magnetometry and resonance Mossbauer spectroscopy for the first time. All layers of bottom sediments contain nanosized single-domain magnetite particles produced by magnetotactic bacteria. The concentration of magnetite in bottom sediments decreased with depth, reaching a local minimum in the layer corresponding to the minimal level of the lake observed in 1910–1930. It is demonstrated that biogenic magnetite may indicate climate-related changes in the level of Shira Lake, in addition to the other biological and geochemical characteristics. © 2016, Pleiades Publishing, Ltd.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T., Zhila N., Kiselev E., Shishatskaya E.
Заглавие : A study of synthesis and properties of poly-3-hydroxybutyrate/diethylene glycol copolymers
Место публикации : Biotechnol. Prog.: John Wiley and Sons Inc., 2016. - Vol. 32, Is. 4. - С. 1017-1028. - ISSN 87567938 (ISSN) , DOI 10.1002/btpr.2267
Ключевые слова (''Своб.индексиров.''): biocompatibility--biosynthesis--molecular weight--physicochemical and mechanical properties--poly(3-hydroxybutyrate)/diethylene glycol copolymers--biochemistry--biocompatibility--biosynthesis--cell culture--cell membranes--cells--cytology--fatty acids--glycols--molecular weight--american institute of chemical engineers--biological properties--chemical compositions--cytoplasmic membrane--degree of saturations--physico-chemical and mechanical properties--physiological effects--poly-3-hydroxybutyrate--biomechanics
Аннотация: This study investigates synthesis of poly(3-hydroxybutyrate)/diethylene glycol copolymers (P3HB/DEG) by Cupriavidus eutrophus B-10646 cells as related to DEG concentration in the medium and the time when it is added to the culture of cells synthesizing P3HB. The study determines the limits of physiological effect of DEG on C. eutrophus cells, showing that at DEG concentrations above 30 g/L, it inhibits cell growth, decreasing cell concentration and total P3HB/DEG yield and inducing an increase in the degree of saturation of fatty acids in lipids of cell cytoplasmic membrane. A series of copolymers containing different molar fractions of DEG (between 0.13 and 3.0 mol%) have been synthesized and their physicochemical, physical/mechanical, and biological properties have been investigated as related to the chemical composition and proportions of DEG monomers of the polymers. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1017–1028, 2016. © 2016 American Institute of Chemical Engineers
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kudryasheva N. S., Petrova A. S., Dementyev D. V., Bondar A. A.
Заглавие : Exposure of luminous marine bacteria to low-dose gamma-radiation
Место публикации : J. Environ. Radioact.: Elsevier Ltd, 2017. - Vol. 169-170. - С. 64-69. - ISSN 0265931X (ISSN) , DOI 10.1016/j.jenvrad.2017.01.002
Ключевые слова (''Своб.индексиров.''): bioassay--low-dose gamma-radiation--luminous marine bacteria--mutagenic effect--radiotoxicity--temperature dependence--bacteria--bioassay--bioluminescence--gamma rays--ionizing radiation--irradiation--phosphorescence--physiological models--radiation effects--temperature distribution--low dose--marine bacterium--mutagenic effect--radiotoxicity--temperature dependence--radiation--bacteria (microorganisms)--photobacterium phosphoreum
Аннотация: The study addresses biological effects of low-dose gamma-radiation. Radioactive 137Cs-containing particles were used as model sources of gamma-radiation. Luminous marine bacterium Photobacterium phosphoreum was used as a bioassay with the bioluminescent intensity as the physiological parameter tested. To investigate the sensitivity of the bacteria to the low-dose gamma-radiation exposure (?250 mGy), the irradiation conditions were varied as follows: bioluminescence intensity was measured at 5, 10, and 20°С for 175, 100, and 47 h, respectively, at different dose rates (up to 4100 ?Gy/h). There was no noticeable effect of gamma-radiation at 5 and 10°С, while the 20°С exposure revealed authentic bioluminescence inhibition. The 20°С results of gamma-radiation exposure were compared to those for low-dose alpha- and beta-radiation exposures studied previously under comparable experimental conditions. In contrast to ionizing radiation of alpha and beta types, gamma-emission did not initiate bacterial bioluminescence activation (adaptive response). As with alpha- and beta-radiation, gamma-emission did not demonstrate monotonic dose-effect dependencies; the bioluminescence inhibition efficiency was found to be related to the exposure time, while no dose rate dependence was found. The sequence analysis of 16S ribosomal RNA gene did not reveal a mutagenic effect of low-dose gamma radiation. The exposure time that caused 50% bioluminescence inhibition was suggested as a test parameter for radiotoxicity evaluation under conditions of chronic low-dose gamma irradiation. © 2017 Elsevier Ltd
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Eremeeva, Elena V., Bartsev, Sergey I., van Berkel, Willem J. H., Vysotski, Eugene S.
Заглавие : Unanimous Model for Describing the Fast Bioluminescence Kinetics of Ca2+-regulated Photoproteins of Different Organisms
Колич.характеристики :8 с
Коллективы : RFBR [14-04-31092]; Russian Academy of Sciences [01201351504, 01201351502]
Место публикации : Photochem. Photobiol.: WILEY, 2017. - Vol. 93, Is. 2. - С. 495-502. - ISSN 0031-8655, DOI 10.1111/php.12664. - ISSN 1751-1097(eISSN)
Примечания : Cited References:55. - This work was supported by RFBR grant 14-04-31092 and the state budget allocated to the fundamental research at the Russian Academy of Sciences (projects 01201351504 and 01201351502).
Предметные рубрики: GREEN-FLUORESCENT PROTEIN
AEQUORIN BIOLUMINESCENCE
SEQUENCE-ANALYSIS
Аннотация: Upon binding their metal ion cofactors, Ca2+-regulated photoproteins display a rapid increase of light signal, which reaches its peak within milliseconds. In the present study, we investigate bioluminescence kinetics of the entire photoprotein family. All five recombinant hydromedusan Ca2+-regulated photoproteinsaequorin from Aequorea victoria, clytin from Clytia gregaria, mitrocomin from Mitrocoma cellularia and obelins from Obelia longissima and Obelia geniculatademonstrate the same bioluminescent kinetics pattern. Based on these findings, for the first time we propose a unanimous kinetic model describing the bioluminescence mechanism of Ca2+-regulated photoproteins.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Dgebuadze, Yu. Yu., Sushchik N. N., Bashinskiy I. V., Makhutova O. N., Kalacheva G. S., Osipov V. V., Gladyshev M. I.
Заглавие : Analysis of fatty acid composition revealed differences in the diets of tadpoles of two amphibian species
Колич.характеристики :4 с
Коллективы : basic research program of the Russian Federation [51.1.1]; Grant Council of the President of the Russian Federation for State Support of Leading Scientific Schools [NSh-9249.2016.5]; Russian Foundation for Basic Research [16-04-01248, 16-34-00119 mol_a]
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2017. - Vol. 472, Is. 1. - С. 31-34. - ISSN 1607-6729, DOI 10.1134/S1607672917010082. - ISSN 1608-3091(eISSN)
Примечания : Cited References:15. - This study was supported by the State task within the framework of the basic research program of the Russian Federation (subject no. 51.1.1), the Grant Council of the President of the Russian Federation for State Support of Leading Scientific Schools (project no. NSh-9249.2016.5), and partly by the Russian Foundation for Basic Research (project nos. 16-04-01248 and 16-34-00119 mol_a).
Предметные рубрики: ECOLOGY
Аннотация: The study of the composition of fatty acid markers of tadpoles of cohabiting amphibian species for the first time revealed differences in their diets: the moor frog Rana arvalis prefers bacteria not associated with plant detritus, whereas the diet of the common spadefoot Pelobates fuscus is based on cyanobacteria, green algae, diatoms, and possibly higher plants. Major differences in the fatty acid composition are determined by the difference in the percentage of eicosapentaenoic and myristic acids.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T. G., Vinogradova O. N., Zhila N. O., Kiselev E. G., Peterson I. V., Vasil’ev A. D., Sukovatyi A. G., Shishatskaya E. I.
Заглавие : Physicochemical properties of multicomponent polyhydroxyalkanoates: Novel aspects
Место публикации : Polym. Sci. Ser. A: Maik Nauka-Interperiodica Publishing, 2017. - Vol. 59, Is. 1. - С. 98-106. - ISSN 0965545X (ISSN) , DOI 10.1134/S0965545X17010163
Ключевые слова (''Своб.индексиров.''): glycols--medical problems--melting--molecular mass--monomers--polymers--chemical compositions--degree of crystallinity--degrees of crystallinity--multi-component polyhydroxyalkanoates--physicochemical property--polyhydroxyalkanoates--temperature intervals--thermal degradation temperatures--organic polymers
Аннотация: The physicochemical properties such as the degree of crystallinity and temperature and molecularmass characteristics of a number of polyhydroxyalkanoates of various chemical composition synthesized on a complex carbon substrate by bacteria Cupriavidus eutrophus В10646 have been investigated. Two-, three-, and four-component copolymer samples have different sets and ratios of monomers with various lengths of carbon chains: 3-hydroxybutyrate (3HB), 4-hydroxybutyrate (4HB), 3-hydroxyvalerate (3HV), 3-hydroxyhexanoate (3HH), 3-hydroxy-4-methyl valerate (3H4MV), and diethylene glycol (DEG). It has been shown that weight-average molar mass Мw and polydispersity vary in a wide range with no correlation existing with the composition of copolymer polyhydroxyalkanoates and that thermal stability is preserved in the temperature interval between the melting temperature and the thermal degradation temperature from 100 to 120–140°С. The composition and ratio of monomers most notably affect the degree of crystallinity of polyhydroxyalkanoates. Significant differences between the degrees of crystallinity of three- and four-component polyhydroxyalkanoates have been found for the first time. The degree of crystallinity for copolymers P(3HB/3HV/4HB) is 9–22%, and the degree of crystallinity for copolymers P(3HB/3HV/3HH) and P(3HB/3GV/3H4MV) is 41–63%; this value is close to the degree of crystallinity for diblock copolymers P(3HB)/DEG, which is 56–69%. For the four-component copolymers P(3HB/3GV/4HB/3HH), the degree of crystallinity is 30–41%. The values of Мw for the copolymers P(3HB/DEG) are inhomogeneous and the polymers contain fractions uneven with respect to molecular mass: a high-molecular-mass polymer (Мw from 2700 to 4900 kDa) and a low-molecular-mass polymer (Мw = 46–167 kDa). For the copolymers P(3HB)/DEG and P(3HB/3HV/3H4MV), two peaks are observed in the region of melting with the gap between these peaks being 4–20°С. All of the types of copolymer samples, regardless of the monomer ratio, show an increase in elongation at break against the background of a decrease in tensile stress and Young’s modulus, with these effects being pronounced to different extents. On the whole, the properties of multicomponent polyhydroxyalkanoates differ appreciably. © 2017, Pleiades Publishing, Ltd.
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18.

Вид документа : Статья из сборника (выпуск продолж. издания)
Шифр издания :
Автор(ы) : Yu Saltykov M., Bartsev S. I.
Заглавие : Developing of discrimination experiment to find most adequate model of plant's multi-nutrient functional response
Место публикации : IOP Conference Series: Materials Science and Engineering: Institute of Physics Publishing, 2017. - Vol. 173: 5th International Workshop on Mathematical Models and their Applications 2016, IWMMA 2016 (7 November 2016 through 9 November 2016, ) Conference code: 126825, Is. 1. - , DOI 10.1088/1757-899X/173/1/012017
Ключевые слова (''Своб.индексиров.''): ecology--metabolism--physiology--adequate models--complex model--discrimination experiments--ecological models--functional response--trophic level--typical patterns--nutrients
Аннотация: To create reliable Closed Ecological Life Support Systems (CELSS) it is necessary to have models which can predict CELSS dynamic with good accuracy. However it was shown that conventional ecological models cannot describe CELSS correctly if it is closed by more than one element. This problem can be solved by means more complex models than conventional ones - so called flexible metabolism models. However it is possible that CELSS also can be described correctly in "semi-conventional" framework - when only one trophic level is described by flexible metabolism model. Another problem in CELSS modeling is existence of different and incompatible hypotheses about relationships between plants growth rate and amounts of nutrients (functional responses). Difficulty of testing these hypotheses is associated with multi-nutrient dependency of growth rate and comprehensive experimental studies are expensive and time-consuming. This work is devoted to testing the hypothesis that "semi-conventional" approach is enough to describe CELSS, and to planning the discrimination experiment on selecting correct type of the plant's functional response. To do that three different models of plants (one flexible and two conventional) were investigated both in the scope of CELSS model, and in hemostat model. Numerical simulations show that each of the models has typical patterns which can be determined in experiment with real plants. © Published under licence by IOP Publishing Ltd.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Yermokhin M. V., Sushchik N. N., Tabachishin V. G., Kalacheva G. S., Kolmakova A. A., Gladyshev M. I.
Заглавие : Amphibia as a Vector of Transfer of Long-Chain Polyunsaturated Omega-3 Fatty Acids from Aquatic to Terrestrial Ecosystems
Колич.характеристики :3 с
Коллективы : Russian Foundation for Basic Research [16-04-01248]; Russian Academy of Sciences [II.2P/VI.51-1]; Ministry of Education and Science of the Russian Federation [6.1504.2017/PCh]; program of the President of the Russian Federation for state support of leading scientific schools [NSh-9249.2016.5]
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2018. - Vol. 481, Is. 1. - С. 195-197. - ISSN 1607-6729, DOI 10.1134/S160767291804004X. - ISSN 1608-3091(eISSN)
Примечания : Cited References:15. - This work was supported by the Russian Foundation for Basic Research (project no. 16-04-01248), project of the Russian Academy of Sciences no. II.2P/VI.51-1, the State Task of the Ministry of Education and Science of the Russian Federation to the Siberian Federal University for research (no. 6.1504.2017/PCh), and the program of the President of the Russian Federation for state support of leading scientific schools (project no. NSh-9249.2016.5).
Предметные рубрики: FATTY-ACIDS
WATER
INSECTS
EXPORT
LAND
EMERGENCE
BIOMASS
FLUXES
Аннотация: The flow of long-chain polyunsaturated fatty acids (PUFAs) of the omega-3 family, namely, eicosapentaenoic acid (20:5n-3, EPA) and docosahexaenoic acid (22:6n-3, DHA), exported by amphibian metamorphs from water to terrestrial ecosystems in the Medveditsa River floodplain, was quantified for the first time. The total biomass export by three amphibian species (Pelobates fuscus, Bombina bombina, and Pelophylax ridibundus) per unit area of the lake surface was 0.594 g/m(2) per year (as a mean for 2 years). The biomass flow per unit area of land was 0.726 g/ha per year (0.302 g/ha per year for organic carbon) in 2015-2016. The average annual total removal of EPA + DHA by amphibians from the floodplain lake was 1.47 mg/m(2) of water surface area. Due to the high content of EPA and DHA in biomass, amphibians are potentially a valuable food for terrestrial predators having no access to other sources of essential PUFAs.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vereshchagina, Kseniya, Kondrateva, Elizaveta, Axenov-Gribanov, Denis, Shatilina, Zhanna, Khomich, Andrey, Bedulina, Daria, Zadereev, Egor, Timofeyev, Maxim
Заглавие : Nonspecific stress response to temperature increase in Gammarus lacustris Sars with respect to oxygen-limited thermal tolerance concept
Колич.характеристики :17 с
Коллективы : Russian Science Foundation [17-14-01063]; Russian Foundation for Basic Research [16-34-00687, 16-34-60060, 17-34-50012]; Goszadanie project joint program of DAAD [6.9654.2017/8.9]; Ministry of education and Science M. Lomonosov [6.12735.2018/12.2]; Lake Baikal Foundation [FOB_02-3/05]
Место публикации : PeerJ: PEERJ INC, 2018. - Vol. 6. - Ст.e5571. - ISSN 2167-8359, DOI 10.7717/peerj.5571
Примечания : Cited References:49. - The study was carried out with the main financial support of Russian Science Foundation grant 17-14-01063, with the partial financial support of Russian Foundation for Basic Research grants 16-34-00687, 16-34-60060, 17-34-50012, the base part of Goszadanie project 6.9654.2017/8.9, joint program of DAAD and Ministry of education and Science M. Lomonosov (6.12735.2018/12.2) and Lake Baikal Foundation (FOB_02-3/05). There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Предметные рубрики: COPEPOD TIGRIOPUS-JAPONICUS
SHUNET SOUTH SIBERIA
HEAT-SHOCK PROTEINS
Ключевые слова (''Своб.индексиров.''): gammarus lacustris--heat shock proteins 70 (hsp70)--nonspecific cellular--stress-response (ncsr)--lactate dehydrogenase--diene conjugates--schiff--bases--triene conjugates
Аннотация: The previously undescribed dynamics of the heat shock protein HSP70 and subsequent lipid peroxidation products have been assessed alongside lactate dehydrogenase activity for Gammarus lacustris Sars, an amphipod species from the saltwater Lake Shira (Republic of Khakassia). Individuals were exposed to a gradual temperature increase of 1 degrees C/hour (total exposure duration of 26 hours) starting from the mean annual temperature of their habitat (7 degrees C) up to 33 degrees C. A complex of biochemical reactions occurred when saltwater G. lactustris was exposed to the gradual changes in temperature. This was characterized by a decrease in lactate dehydrogenase activity and the launching of lipid peroxidation. The HSP70 level did not change significantly during the entire experiment. In agreement with the concept of oxygen-limited thermal tolerance, an accumulation of the most toxic lipid peroxides (triene conjugates and Schiff bases) in phospholipids occurred at the same time and temperature as the accumulation of lactate. The main criterion overriding the temperature threshold was, therefore, the transition to anaerobiosis, confirmed by the elevated lactate levels as observed in our previous associated study, and by the development of cellular stress, which was expressed by an accumulation of lipid peroxidation products. An earlier hypothesis, based on freshwater individuals of the same species, has been confirmed whereby the increased thermotolerance of G. lacustris from the saltwater lake was caused by differences in energy metabolism and energy supply of nonspecific cellular stress-response mechanisms. With the development of global climate change, these reactions could be advantageous for saltwater G. lacustris. The studied biochemical reactions can be used as biomarkers for the stress status of aquatic organisms when their habitat temperature changes.
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