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BIOLUMINESCENT ANALYSIS - THE ACTION OF TOXICANTS - PHYSICAL-CHEMICAL REGULARITIES OF THE TOXICANTS EFFECTS [Text] / N. S. KUDRYASHEVA, V. A. KRATASYUK, P. I. BELOBROV // Anal. Lett. - 1994. - Vol. 27, Is. 15. - P2931-2947. - Cited References: 13 . - 17. - ISSN 0003-2719

РУБ Chemistry, Analytical

Кл.слова (ненормированные):
BACTERIAL LUCIFERASE BIOTEST -- FOREIGN COMPOUNDS -- ENERGY OF ELECTRON EXCITED STATES LEVEL -- REDOX POTENTIAL -- REDUCING OF BIOLUMINESCENT INTENSITY -- INDUCTION PERIOD -- TIME OF MAXIMUM LIGHT INTENSITY
Аннотация: The physical-chemical regularities of aromatic compounds' effects in luciferase to toxicity biotesting have been studied, The structures and physical-chemical characteristics of the toxicants and of the bioluminescent emitter were taken into account. The inhibition constants of bioluminescence intensity (I) were calculated and interpreted from the viewpoint of the energy (electron) transfer processes. The induction period (P) and the increase of the rime of the maximum light intensity (t(M)) which take place in the quinones presence, have been shown to deal with hydrogen transfer processes. The values of I, P and t(M) have been shown to be connected with a size of the quinones' aromatic and aliphatic parts, P- and t(M)-dependencies on quinone's redox potential have been demonstrated.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
KUDRYASHEVA, N.S.; KRATASYUK, V.A.; BELOBROV, P.I.

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Patterns of inhibition of bacterial bioluminescence in vitro by quinones and phenols - Components of sewage / N. S. Kudryasheva [et al.] // Biophysics. - 1994. - Vol. 39, Is. 3. - P. 441-451 . - ISSN 0006-3509
Аннотация: Quenching of bioluminescence of the bienzyme system bacterial luciferase-NAD В· H:FMN-oxidoreductaseвЂ вЂ NADВ·H is reduced nicotinamide adenine dinucleotide and FMN is flavine manonucleotide. is considered in terms of the patterns of intermolecular transfer of an electron and hydrogen between donor (bioluminescent centre) and acceptor (compound to be analysed) taking into account the structure of the acceptor molecule. The link between the induction period of bioluminescence and the redox potential of the quinone-acceptor has been confirmed. It is shown that the induction period and the time of passage to the bioluminescence maximum in the presence of quinones are determined by the size of the aromatic and aliphatic fragments of the quinone molecule; the intensity of bioluminescence depends on the efficiency of the processes of intermolecular electron transfer (donor-acceptor) and the competition of the compound being analysed with aldehyde for the binding site on luciferase. В© 1994.

Scopus
Держатели документа:
Institute of Biophysics, the Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Irkutsk State University Biology Research Institute, Irkutsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kudryasheva, N.S.; Shalayeva, Ye.V.; Zadorozhnaya, Ye.N.; Kratasyuk, V.A.; Balayan, A.E.; Stom, D.I.

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Properties of recombinant fluorescent proteins from Photobacterium leiognathi and their interaction with luciferase intermediates / V. N. Petushkov, B. G. Gibson, J. Lee // Biochemistry. - 1995. - Vol. 34, Is. 10. - P3300-3309 . - ISSN 0006-2960
Кл.слова (ненормированные):
luciferase -- recombinant protein -- article -- ligand binding -- nonhuman -- priority journal -- protein isolation -- protein protein interaction -- protein stability -- vibrionaceae -- Bacterial Proteins -- Binding Sites -- Carrier Proteins -- Circular Dichroism -- Flavin Mononucleotide -- Fluorescence Polarization -- Genes, Bacterial -- Kinetics -- Ligands -- Luciferase -- Luminescence -- Molecular Sequence Data -- Photobacterium -- Recombinant Proteins -- Spectrophotometry -- Support, U.S. Gov't, P.H.S. -- Photobacterium leiognathi -- Vibrionaceae
Аннотация: Ligand binding and luciferase interaction properties of the recombinant protein corresponding to the lumazine protein gene (EMBL X56534) of Photobacterium leiognathi have been determined by fluorescence dynamics, circular dichroism, gel filtration, and SDS-PAGE. Scatchard analysis of a fluorescence titration shows that the apoprotein possess one binding site, and at 30В°C the KdS (?M) are as follows: 6,7-dimethyl-8-ribityllumazine, 0.26; riboflavin, 0.53; and much more weakly bound FMN, 30. All holoproteins are highly fluorescent and have absorption spectra distinct from each other and from the free ligands. The longest wavelength absorption maxima are, respectively (nm, 2В°C), 420,463, and 458. Ligand binding produces no change in the far-UV circular dichroism; all have mean residual ellipticity at 210 nm of -6500 deg cm2 dmol-1, the same as the native protein. However, in the bioluminescence reaction only the lumazine holoprotein shows a bioluminescence effect. Fluorescence emission anisotropy decay was used to establish that none of these holoproteins complexed with native luciferase and that the lumazine protein alone formed a 1:1 complex with the luciferase hydroxyflavin fluorescent transient and the luciferase peroxyflavin intermediates, revealed by a dominant channel of anisotropy loss, with rotational correlation time of 2.5 ns, and attributed to excitation transfer from the luciferase flavin donor to the acceptor, the lumazine ligand. The complex stability was sufficient to allow its isolation by FPLC gel filtration and verification by SDS-PAGE. These methods also confirmed the absence of interaction of the holoflavoproteins.

Scopus
Держатели документа:
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, United States
Institute of Biophysics, Academy of Sciences of Russia (Siberian Branch), 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V.N.; Gibson, B.G.; Lee, J.

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Interaction of Photobacterium leiognathi and Vibrio fischeri Y1 luciferases with fluorescent (antenna) proteins: Bioluminescence effects of the aliphatic additive / V. N. Petushkov [et al.] // Biochemistry. - 1996. - Vol. 35, Is. 37. - P12086-12093, DOI 10.1021/bi9608931 . - ISSN 0006-2960
Кл.слова (ненормированные):
luciferase -- anisotropy -- antenna -- article -- bioluminescence -- complex formation -- energy transfer -- enzyme active site -- enzyme kinetics -- nonhuman -- priority journal -- protein protein interaction -- spectroscopy -- vibrionaceae -- Bacterial Proteins -- Carrier Proteins -- Cloning, Molecular -- Dithionite -- Flavin Mononucleotide -- Kinetics -- Luciferases -- Luminescent Measurements -- Luminescent Proteins -- Models, Structural -- Photobacterium -- Protein Binding -- Protein Conformation -- Recombinant Proteins -- Spectrophotometry -- Vibrio -- Bacteria (microorganisms) -- Photobacterium -- Photobacterium leiognathi -- Vibrio fischeri -- Vibrionaceae
Аннотация: The kinetics of the bacterial bioluminescence reaction is altered in the presence of the fluorescent (antenna) proteins, lumazine protein (LumP) from Photobacterium or the yellow fluorescence proteins (YFP) having FMN or Rf bound, from Vibrio fischeri strain Y1. Depending on reaction conditions, the bioluminescence intensity and its decay rate may be either enhanced or strongly quenched in the presence of the fluorescent proteins. These effects can be simply explained on the basis of the same protein-protein complex model that accounts for the bioluminescence spectral shifts induced by these fluorescent proteins. In such a complex, where the fluorophore evidently is in proximity to the luciferase active site, it is expected that the on off rate of certain aliphatic components of the reaction should be altered with a consequent shift in the equilibria among the luciferase intermediates, as recently elaborated in a kinetic scheme. These aliphatic components are the bioluminescence reaction substrate, tetradecanal or other long-chain aldehyde, its carboxylic acid product, or dodecanol used as a stabilizer of the luciferase peroxyflavin. No evidence can be found for the protein- protein interaction in the absence of the aliphatic component.

Scopus
Держатели документа:
Department of Biochemistry, University of Georgia, Athens, GA 30602, United States
Institute of Biophysics, Acad. of Sci. of Russia, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V.N.; Ketelaars, M.; Gibson, B.G.; Lee, J.

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Photosynthetic apparatus of cucumber and pea plants grown under red light with discrete or continuous spectra / E. N. Zavorueva [et al.] // Russian Journal of Plant Physiology. - 1996. - Vol. 43, Is. 2. - P191-199 . - ISSN 1021-4437
Кл.слова (ненормированные):
And thermoinduced changes in fluorescence yield -- Cucumis sativum -- Photo -- Photophosphorylation -- Pigments -- Pisum sativum
Аннотация: The effect of light sources with discrete or continuous spectra of red light (50 W/m2, 600-700 nm) on the structural and functional characteristics of chloroplasts were studied in leaves of cucumber (Cucumis sativum L.) plants, which die under strong red light, and pea (Pisum sativum L.) plants, which tolerate red light under the same conditions. Leaf condition was assessed by measuring thermo-and photoinduced changes in the chlorophyll fluorescence yield and the photochemical and photophosphorylating activities of the chloroplasts. Different responses of the pigment apparatus of pea and cucumber plants to red light with continuous or line spectra were revealed. Pea plants responded to discrete-spectrum light by changing P700 content, the ratio of antenna chlorophyll to P700, and the position of the low-temperature peak of the temperature-induced chlorophyll fluorescence yield. In cucumber plants, disturbances in the energy transfer from the short-wavelength pigments to chlorophyll a were observed. In both plants, the effects of line spectrum light on the pigment apparatus were reversible; the ratio of cyclic to noncyclic photophosphorylation, photosynthetic control, and the extent of the coupling of photosynthetic electron transport to photophosphorylation did not change compared to the control light. The need for examining the line spectra of light sources for growing plants under moderate intensities of artificial light (about 50 W/m2 of photosynthetically active radiation) is discussed.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zavorueva, E.N.; Nesterenko, T.V.; Volkova, E.K.; Tikhomirov, A.A.

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Direct measurement of excitation transfer in the protein complex of bacterial luciferase hydroxyflavin and the associated yellow fluorescence proteins from Vibrio fischeri Y1 [Text] / V. N. Petushkov, B. G. Gibson, J. . Lee // Biochemistry. - 1996. - Vol. 35, Is. 25. - P8413-8418, DOI 10.1021/bi952691v. - Cited References: 24 . - ISSN 0006-2960

РУБ Biochemistry & Molecular Biology
Рубрики:
LUMAZINE PROTEIN
   LUMINOUS BACTERIUM
   STRAIN Y-1
   BIOLUMINESCENCE
   EMISSION
   PURIFICATION
   TRANSIENT
   LIGHT
Аннотация: Time-resolved fluorescence was used to directly measure the energy transfer rate constant in the protein-protein complex involved in the yellow bioluminescence of Vibrio fischeri, strain Y1. In this reaction the putative donor is the fluorescent transient intermediate, luciferase hydroxyflavin, which exhibits a major fluorescence lifetime of the bound flavin of 10 ns. On addition of the acceptor, the V. fischeri yellow fluorescence protein containing either FMN or riboflavin as ligand, a rapid decay time, 0.25 ns, becomes predominant. The same results are observed using rec-luciferase from Photobacterium leiognathi to produce the donor. Because of favorable spectral separation in this system, this rapid decay rate of 4 ns(-1), can be directly equated to the energy transfer rate. This rate is ten times higher than the rate previously observed in the Photobacterium luciferase hydroxyflavin-lumazine protein, donor-acceptor system, derived from emission anisotropy measurements. This ten-times ratio is close to the ratio of spectral overlaps of the donor fluorescence with the acceptor absorption, between these two systems, so it is concluded that the topology of the protein complexes in both cases, must be very similar. Energy transfer is also monitored by the loss of steady-state fluorescence intensity at 460 nm of the donor, on addition of the acceptor protein. A fluorescence titration indicates that luciferase hydroxyflavin and the yellow protein complex with a 1:1 stoichiometry with a K-d of 0.7 mu M (0 degrees C). These parameters account for the bioluminescence spectral shifting effects observed in these reactions.

Держатели документа:
UNIV GEORGIA,DEPT BIOCHEM & MOLEC BIOL,ATHENS,GA 30602
RUSSIAN ACAD SCI,INST BIOPHYS,SIBERIAN BRANCH,KRASNOYARSK 660036,RUSSIA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V.N.; Gibson, B.G.; Lee, J...

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Brief exposures of resting fibroblasts to okadaic acid stimulate DNA synthesis [Text] / N. A. Setkov, O. I. Epifanova // Cell Prolif. - 1997. - Vol. 30, Is. 1. - P. 7-19, DOI 10.1111/j.1365-2184.1997.tb00912.x. - Cited References: 32 . - ISSN 0960-7722

РУБ Cell Biology
Рубрики:
PDGF-INDUCED PROLIFERATION
   HAMSTER-EMBRYO CELLS
   PROTEIN-PHOSPHORYLATION
   PHOSPHATASE INHIBITORS
   MOUSE FIBROBLASTS
   3T3 CELLS
   FUSION
   TRANSFORMATION
   INDUCTION
   ARREST
Аннотация: To study further the factors providing for cellular quiescence, we used okadaic acid (OA) at concentrations (0.1, 1, 10 or 100 nM) inhibiting type 1 and/or type 2A protein phosphatases in mammalian cell cultures. Brief (2 h) exposure of resting (0.2% serum for 72 h) NIH 3T3 mouse fibroblasts to OA with subsequent incubation of cells in a medium with 0.2% serum, stimulated DNA synthesis at all concentrations studied. Maximal stimulation was observed following pre-incubation of resting cells with 10 nM OA. Treatment of cycling cells (10% serum) with OA (2 h pulses at 12 h intervals for 72 h) prevented their exit to the resting state on transfer to a medium with 0.2% serum. Brief exposures of resting cells to OA did not affect the rate of protein synthesis. OA pulses in the late pre-replicative period had no effect on the entry of serum-stimulated cells into the S phase. Cell fusion experiments with resting (serum-deprived) and proliferating (serum-stimulated) NIH 3T3 cells, using radioautography with a double-labelling technique, revealed that pre-incubation of resting cells with OA for 2 h before and after fusion abrogates their ability to suppress the onset of DNA synthesis in the nuclei of proliferating cells in heterodikaryons. The results indicate that protein phosphatases of type 1 and/or 2A may be involved in the growth-arrest machinery that provides for cellular quiescence.

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Держатели документа:
VA ENGELHARDT MOL BIOL INST,MOSCOW 117984,RUSSIA
RUSSIAN ACAD SCI,INST BIOPHYS,SIBERIAN BRANCH,KRASNOYARSK,RUSSIA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Setkov, N.A.; Epifanova, O.I.

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Transfer of xenobiotics through cell membranes of luminous bacteria / S. E. Medvedeva // Luminescence. - 1999. - Vol. 14, Is. 5. - P267-270 . - ISSN 1522-7235
Кл.слова (ненормированные):
Luminous bacteria -- Toxicant -- Ultrastructure -- bacterial DNA -- edetic acid -- toluene -- xenobiotic agent -- article -- cell membrane -- DNA damage -- drug effect -- luminescence -- metabolism -- Photobacterium -- sensitivity and specificity -- transport at the cellular level -- ultrastructure -- Vibrio -- Biological Transport -- Cell Membrane -- DNA Damage -- DNA, Bacterial -- Edetic Acid -- Luminescence -- Photobacterium -- Sensitivity and Specificity -- Toluene -- Vibrio -- Xenobiotics
Аннотация: The influence of some chemical substances on luminous bacteria was studied to elucidate the interrelation between the xenobiotics action on bacterial luminescence and cell ultrastructure. Such substances as quinones, phenols, chlorides of heavy metals (in concentrations of substances inhibiting luminescence by 50%) resulted in damaging effects upon bacteria: a lot of cells had damage of membranes due to changes in their permeability. It was found that the high concentration of EDTA and toluene decreased the luminescence and caused the condensation of DNA-fibrils and the cell damage after long-term and short-term action. The low concentration of EDTA and toluene did not decrease the bacterial luminescence; the noticeable damage of cell membranes did not take place during short-term treatment. However, the long action of these substances changed the membrane permeability resulting in increased sensitivity of bacterial luminescence to some toxic substances. Copyright В© 1999 John Wiley & Sons, Ltd.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Medvedeva, S.E.

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Mechanisms of the effect of xenobiotics on bacterial bioluminescence [Text] / N. S. Kudryasheva // Luminescence. - 1999. - Vol. 14: 10th International Symposium on Bioluminescence and Chemiluminescence (1998, BOLOGNA, ITALY), Is. 4. - P. 199-200, DOI 10.1002/(SICI)1522-7243(199907/08)14:4199::AID-BIO5303.0.CO;2-X. - Cited References: 12 . - ISSN 1522-7235

РУБ Biochemistry & Molecular Biology

Кл.слова (ненормированные):
xenobiotics -- bioluminescence quenching -- energy -- electron and hydrogen transfer
Аннотация: The influence of xenobiotics on the bioluminescent enzyme system is considered in terms of molecular action on the primary physicochemical processes-energy, electron and hydrogen (e(-) + H+) transduction. Dyes, non-fluorescent chemically inert organic compounds, redox-active organic compounds and metallic salts were investigated. The influence of the different xenobiotics depends in a complex way on physicochemical characteristics of the xenobiotic molecules (spectral-luminescent characteristics, electron-acceptation energy and redox potential). Copyright (C) 1999 John Wiley & Sons, Ltd.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, SB, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kudryasheva, N.S.

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10.

On the quenching of bacterial luminescence by dyes [Текст] / Y. P. Meshalkin [и др.] // Biofizika. - 1999. - Vol. 44, Is. 6. - P. 1083-1087. - Cited References: 5 . - ISSN 0006-3029

РУБ Biophysics

Кл.слова (ненормированные):
bacterial luminescence -- dyes -- quenching
Аннотация: It was shown that the addition of dyes (sodium fluorescein, rhodamine 6G, unsubstituted rhodamine) to a bienzymic reaction mixture. (luciferin-luciferase. complex): leads to a decrease: in fluorescence intensity and the appearance of the dye fluorescence band. A similar effect was observed when the luciferin-luciferase complex and dye molecules were separated by distances considerably. exceeding the Forster radius of transfer. It is assumed that the mechanism of dye. fluorescence is not related to the excitation energy resonance transfer but is based on the excitation of dye molecules due to direct absorption of quanta of bacterial bioluminescence.

WOS
Держатели документа:
Novosibirsk State Tech Univ, Novosibirsk, Russia
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Meshalkin, Y.P.; Nemtseva, E.V.; Alfimov, E.E.; Kudryasheva, N.S.

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11.

Evaluation of the effect of light intensity on the measurement of the photosynthetic rate in plankton microalgae by the chlorophyll fluorescence method [Text] / N. A. Gaevskii [et al.] // Russ. J. Plant Physiol. - 2000. - Vol. 47, Is. 6. - P. 820-825, DOI 10.1023/A:1026671531500. - Cited References: 21 . - ISSN 1021-4437

РУБ Plant Sciences
Рубрики:
QUANTUM YIELDS
   PHYTOPLANKTON
   CHLOROPLASTS
   ALGAE
Кл.слова (ненормированные):
fluorescence -- chlorophyll -- microalgae -- photosynthetic rate -- photosynthetic activity
Аннотация: The use of relative variable fluorescence (RVF) of chlorophyll, as measured in the presence of Diuron, an inhibitor of electron transfer, for the estimation of the photosynthetic activity of plankton microalgae was analyzed under a wide range of light intensities in the PAR region. Oxygen evolution rates (estimated by the method of light and dark bottles and the amperometric method), RVF, and chlorophyll a concentration were measured in parallel in natural algal cenoses and microecosystems. When the previously used regression equation, in the form A = b(DeltaF/F-d)CchlI, where A is O-2 evolution rate (g/(m(3) h), DeltaF/F-d is RVF (relative units), C-chl is chlorophyll a concentration (mg/m(3)), and I is light intensity (W/m(2)), was verified in the PAR region, we observed a nonlinear dependence of the correction coefficient b on I, which can be described by the formula b = 6.227 x 10(3)rootI. This result agrees with the hypothesis that chlorophyll a fluorescence quenching comprises photochemical (qQ) and energy (qE) components. On the basis of the energy model, we determined the upper limit b(max) = 0.003 for light intensity range I < 4.4 W/m(2) and the lower limit b(min) = 0.0003 for I = 400 W/m(2).

WOS
Держатели документа:
Krasnoyarsk State Univ, Dept Biol, Krasnoyarsk 660036, Russia
Russian Acad Sci, Siberian Div, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gaevskii, N.A.; Kolmakov, V.I.; Popel'nitskii, V.A.; Gold, V.M.; Dubovskaya, O.P.

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12.

Closed artificial ecosystems as a means of ecosystem studies for earth and space needs / N. S. Pechurkin, I. M. Shirobokova // Advances in Space Research. - 2001. - Vol. 27, Is. 9. - P1497-1504, DOI 10.1016/S0273-1177(01)00244-7 . - ISSN 0273-1177
Кл.слова (ненормированные):
artificial ecosystem -- bioremediation -- biosphere -- ecosystem -- environmental monitoring -- model -- Bioremediation -- Ecology -- Ecosystems -- Health -- Biosphere -- Space research -- artificial ecosystem -- Biodegradation, Environmental -- Earth (Planet) -- Ecological Systems, Closed -- Ecology -- Ecosystem -- Energy Transfer -- Environmental Microbiology -- Life Support Systems -- Population Dynamics -- Yeasts
Аннотация: Closed Artificial ecosystems (CAES) have good prospects for wide use as new means for quantitative studies of different types of both natural ecosystems and man-made ones. The paper deals with the discussion of three points of CAES applications. The first one is of importance for theoretical ecology development and is connected with bringing together В«holisticВ» and В«merologicalВ» approaches in ecosystems studies. Using CAES, we can combine both approaches, taking into account the biotic turnover of limiting substrates which few in number even for complicated natural ecosystems. The second CAES use concerns the development of В«ecosystems healthВ» concept and application of a key-factor-approach for the indication and measurement of healthy unhealthy state and functioning of ecosystems or their links. The third use is more of an applied nature, oriented to the intensification of bioremediation or biodepollution processes in different types of ecosystems, including the global biosphere. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, 660036, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pechurkin, N.S.; Shirobokova, I.M.

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13.

Surface bonding states of nano-crystalline diamond balls / J. L. Peng [et al.] // International Journal of Modern Physics B. - 2001. - Vol. 15, Is. 31. - P4071-4085, DOI 10.1142/S0217979201007865 . - ISSN 0217-9792
Кл.слова (ненормированные):
diamond -- article -- crystal structure -- electron -- energy transfer -- nanoparticle -- particulate matter -- structure analysis -- surface property -- transmission electron microscopy
Аннотация: The rough surface of nano-crystalline diamond spheres induces surface electronic states which appear as a broadened pre-peak over approx. 15 eV at the C K-edge energy threshold for carbon in the parallel electron energy loss spectrum (PEELS). This appears to be at least partially due to 1s-?* transitions, although typically the latter occupy a range of only 4 eV for the sp2 edge of highly-oriented pyrollytic graphite (HOPG). No ?* electrons appear in the conduction band inside the diamond particles, where all electrons are sp3 hybridized. PEELS data were also obtained from a chemical vapour deposited diamond film (CVDF) and gem-quality diamond for comparison with the spectra of nano-diamonds. The density of Sp2 and Sp3 states on the surface of diamond nano-crystals is calculated for simple structural models of the diamond balls, including some conjecture about surface structures. The results are used to interpret the sp2/sp3 ratios measured from the PEELS spectra recorded as scans across the particles. Surface roughness at the atomic scale was also examined using high-resolution transmission electron microscopy (HRTEM) and electron nano-diffraction patterns were used to confirm the crystal structures.

Scopus
Держатели документа:
Department of Applied Physics, RMIT University, Swanston Street, Melbourne, Vic. 3051, Australia
Electron Microscope Unit, University of Sydney, NSW 2006, Australia
Molecular Architecture Group, Kirensky Institute of Physics, Institute of Biophysics, 660036 Krasnoyarsk, Russian Federation
School of Physics, University of Melbourne, Parkville, Vic. 3010, Australia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Peng, J.L.; Bulcock, S.; Belobrov, P.I.; Bursill, L.A.

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14.

Upper electron-excited states in bioluminescence: experimental indication [Text] / N. S. Kudryasheva [et al.] // Luminescence. - 2001. - Vol. 16, Is. 3. - P. 243-246, DOI 10.1002/bio.613. - Cited References: 22 . - ISSN 1522-7235

РУБ Biochemistry & Molecular Biology

Кл.слова (ненормированные):
bioluminescence -- upper electron-excited states -- energy transfer
Аннотация: The involvement of upper electron-excited states in bacterial bioluminescence process was studied with excitation energy-accepting molecules. The fluorescent aromatic compounds, anthracene and 1.4-bis(5-phenyloxazol-2-yl)benzene, were chosen. Energies of their lowest excited singlet states are higher than the energy of the analogous state of the bioluminescence emitter; their absorption spectra and bioluminescence do not overlap. Hence, the excitation of these molecules by singlet-singlet energy transfer or by light absorption is excluded. Sensitized fluorescence of these compounds in the bioluminescence systems has been recorded, indicating the activity of upper electron-excited states in the bioluminescent process. Copyright (C) 2001 John Wiley & Sons, Ltd.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, SB, Krasnoyarsk 660036, Russia
Novosibirsk State Tech Univ, Novosibirsk 630092, Russia
Krasnoyarsk State Univ, Dept Phys, Krasnoyarsk 660041, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kudryasheva, N.S.; Nemtseva, E.V.; Meshalkin, Y.P.; Sizykh, A.G.

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15.

Predictive radioecological mathematical model of the Yenisei river / A. G. Degermendzhi, L. G. Kosolapova, V. M. Belolipetskij // Radiatsionnaya Biologiya. Radioekologiya. - 2002. - Vol. 42, Is. 4. - С. 433-439 . - ISSN 0869-8031
Кл.слова (ненормированные):
Cesium -- Contamination -- Mathematical models -- Phosphorus -- Radioisotopes -- River pollution -- Cesium 137 -- Phosphorus 32 -- Radioecology -- The Yenisei river -- Ecosystems -- Bacteria (microorganisms) -- fresh water -- article -- ecosystem -- pollutant -- Russian Federation -- theoretical model -- Ecosystem -- Fresh Water -- Models, Theoretical -- Radioactive Pollutants -- Russia
Аннотация: A one-dimensional mathematical model of the Yenisei river ecosystem including hydrological, ecosystem and radioecologicl blocks has been developed. The model was used to evaluate contribution of different processes (transfer by water masses, dilution, radioactive decay, bioaccumulation) into self-purification of the river water from a radiation pollution. The pollution density of ecosystem components (bacteria, phyto-, zooplankton, phyto-, zoobenthos, detritus) with 137Cs and 32P is calculated.

Scopus
Держатели документа:
Inst. of Biophysics, Siberian Branch, RAS, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Degermendzhi, A.G.; Kosolapova, L.G.; Belolipetskij, V.M.

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16.

Geographical and seasonal distribution of multiple antibiotic resistance of heterotrophic bacteria of Lake Shira / T. I. Lobova [et al.] // Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - P299-307, DOI 10.1023/A:1015618820450 . - ISSN 1386-2588
Кл.слова (ненормированные):
Ampicillin -- Brackish lake -- Halotolerance -- Heterotrophic bacteria -- Horizontal gene transfer -- Kanamycin -- Multiple antibiotic resistance -- antibiotics -- bacterium -- drug resistance -- geographical distribution -- saline lake -- salinity tolerance -- seasonal variation -- Russian Federation -- Bacteria (microorganisms)
Аннотация: From 1996 to 1999 heterotrophic bacteria of the brackish-water Lake Shira (Republic of Khakasia, Russia) were studied to understand the seasonal dynamics of their antibiotic resistance. During the winter, these bacteria were represented primarily by forms that could not be cultured and were psychrotolerant. In the summer period, heterotrophic, mesophilic bacteria increased in number. The percentages of isolates with multiple, antibiotic resistance isolated from the lake region near the resort area of the lake were 2-3 times higher than those from the central part of the lake. A decline in the bacterial numbers with multiple antibiotic resistance was observed during the cold period (February-March). Various mechanisms of multiple, antibiotic resistance of heterotrophic bacteria isolated from Shira lake are discussed.

Scopus
Держатели документа:
Institute of Biophysics of SB RAS, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Lobova, T.I.; Maksimova, E.Ye.; Popova, L.Yu.; Pechurkin, N.S.

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17.

Estimation of energy of the upper electron-excited states of the bacterial bioluminescent emitter [Text] / N. S. Kudryasheva [et al.] // J. Photochem. Photobiol. B-Biol. - 2002. - Vol. 68, Is. 02.03.2013. - P. 88-92, DOI 10.1016/S1011-1344(02)00360-3. - Cited References: 25 . - ISSN 1011-1344

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
MECHANISM
Кл.слова (ненормированные):
bioluminescence -- electron-excited states -- energy transfer
Аннотация: The hypothesis of activity of the upper electron-excited states of the bacterial bioluminescent emitter was verified using dye molecules as foreign energy acceptors. Six compounds were selected having fluorescent state energies ranging from 25 700 to 32 000 cm(-1) (anthracene, pyrene, 1.4-bis(5-phenyloxasol-2-yl)benzene (POPOP), p-bis(o-methylstyryl)benzene (MSB), 2-methoxy-naphtalene, p-terphenyl), exceeding that of the bioluminescent emitter (22 000 cm(-1)). Their absorption spectra do not overlap with the bioluminescence spectrum; the trivial light absorption and the intermolecular resonance S-S energy transfer were excluded. Bacterial bioluminescent spectra of the coupled enzyme system NADH:FMN-oxidoreductase-luciferase in the presence of MSB were presented as an example. The weak sensitized fluorescence of MSB was registered. The results obtained have confirmed the activity of the energetic precursor in the bacterial bioluminescence. Its energy can be located in the interval of 26 000-27 000 cm(-1). (C) 2002 Published by Elsevier Science B.V.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Akademgorodok 660036, Krasnoyarsk, Russia
Krasnoyarsk State Univ, Krasnoyarsk 660049, Russia
Wageningen Univ, Microspect Ctr, Dept Biomol Sci, NL-6703 HA Wageningen, Netherlands
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kudryasheva, N.S.; Nemtseva, E.V.; Sizykh, A.G.; Kratasyuk, V.A.; Visser, AJWG

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18.

Kinetic Parameters of a Culture of the Hydrogen-Oxidizing Bacterium Ralstonia eutropha Grown under Conditions Favoring Polyhydroxybutyrate Biosynthesis / T. G. Volova, N. A. Voinov // Applied Biochemistry and Microbiology. - 2003. - Vol. 39, Is. 2. - P166-170, DOI 10.1023/A:1022585912873 . - ISSN 0003-6838
Кл.слова (ненормированные):
hydrogen -- poly(3 hydroxybutyric acid) -- article -- bacterial growth -- bacterium culture -- biosynthesis -- controlled study -- culture medium -- density -- fermentation -- gas -- gravimetry -- heat exchange -- nonhuman -- oxidation -- Ralstonia eutropha -- surface tension -- temperature dependence -- thermogenesis -- Bacteria (microorganisms) -- Ralstonia -- Wautersia eutropha
Аннотация: Kinetic parameters of a culture of the hydrogen-oxidizing bacterium Ralstonia eutropha grown on a gas substrate under conditions favoring autotrophic biosynthesis of polyhydroxybutyrate were studied. The following parameters, making it possible to control and optimize the process in industrial situations, were determined: specific rate of substrate consumption, physical properties of the culture medium, and coefficients of heat emission and mass transfer.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660033, Russian Federation
Siberian State Technol. University, Min. of Educ. of the Russ. Fed., Krasnoyarsk, 660049, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Voinov, N.A.

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19.

System analysis of links interactions and development of ecosystems of different types / N. S. Pechurkin, I. M. Shirobokova // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1667-1674, DOI 10.1016/S0273-1177(03)80013-3 . - ISSN 0273-1177
Кл.слова (ненормированные):
Free energy -- Heuristic methods -- Hierarchical systems -- Mathematical models -- Photosynthesis -- Systems analysis -- Biological interactions -- Ecosystems -- anthropogenic effect -- ecosystem function -- systems analysis -- article -- biological model -- biomass -- ecology -- ecosystem -- energy transfer -- environmental protection -- food chain -- methodology -- microclimate -- plankton -- population dynamics -- statistics -- Biomass -- Conservation of Natural Resources -- Ecological Systems, Closed -- Ecology -- Ecosystem -- Energy Transfer -- Food Chain -- Models, Biological -- Plankton -- Population Dynamics
Аннотация: The anthropogenic impact on the Earth's ecosystems are leading to dramatic changes in ecosystem functioning and even to destruction of them. System analysis and the use of heuristic modeling can be an effective means to determine the main biological interactions and key factors that are of high importance for understanding the development of ecosystems. Cycling of limiting substances, induced by the external free energy flux, and trophic links interaction is the basis of the mathematical modeling studies presented in this paper. Mathematical models describe the dynamics of simplified ecosystems having different characteristics: 1) different degrees of biotic turnover closure (from open to completely closed); 2) different numbers of trophic links (including both "topdown", "bottom-up" regulation types); 3) different intensities of input - output flows of the limiting nutrient and its total amount in the system. Adaptive values of the changes of lower hierarchical levels (populational, trophic chain level) are to be estimated by integrity indices for total system functioning (e.g. NPP, total photosynthesis). The approach developed can be used for evaluating the contributions of lower hierarchical levels to the functioning of the higher hierarchical levels of the system. This approach may have value for determining biomanipulation management and their assessment. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, SB RAS, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pechurkin, N.S.; Shirobokova, I.M.

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20.

Kinetic parameters of a culture of the hydrogen-oxidizing Ralstonia eutropha, grown under the regimen of biosynthesis of polyhydroxybutyrate / T. G. Volova, N. A. Voinov // Prikladnaia biokhimiia i mikrobiologiia. - 2003. - Vol. 39, Is. 2. - С. 189-193 . - ISSN 0555-1099
Кл.слова (ненормированные):
hydrogen -- hydroxybutyric acid -- polyester -- article -- culture medium -- enzyme specificity -- growth, development and aging -- kinetics -- metabolism -- oxidation reduction reaction -- temperature -- Wautersia eutropha -- Culture Media -- Cupriavidus necator -- Hydrogen -- Hydroxybutyrates -- Kinetics -- Oxidation-Reduction -- Polyesters -- Substrate Specificity -- Temperature
Аннотация: Kinetic parameters of a culture of the hydrogen-oxidizing bacterium Ralstonia eutropha, grown on a gas substrate under the conditions favoring autotrophic biosynthesis of polyhydroxybutyrate, were studied. The following parameters, making it possible to control and optimize the process in industrial situations, were determined: specific rate of substrate consumption, physical properties of culture medium, and coefficients of heat emission and mass transfer.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Voinov, N.A.

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