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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Purtov K.V., Petunin A.I., Burov A.E., Puzyr A.P., Bondar V.S.
Заглавие : Nanodiamonds as Carriers for Address Delivery of Biologically Active Substances
Колич.характеристики :6 с
Коллективы :
Место публикации : Nanoscale Res. Lett.: SPRINGER, 2010. - Vol. 5, Is. 3. - С. 631-636. - ISSN 1931-7573, DOI 10.1007/s11671-010-9526-0
Примечания : Cited References: 24. - This work was supported by the Program # 27 for Basic Research of the Presidium of RAS (project 3.6.3).
Предметные рубрики: ANTICANCER DRUGS
NANOPARTICLES
ADSORPTION
PARTICLES
PROTEINS
Ключевые слова (''Своб.индексиров.''): nanodiamonds--ligand--protein immobilization--nanocarrier--targeted delivery
Аннотация: Surface of detonation nanodiamonds was functionalized for the covalent attachment of immunoglobulin, and simultaneously bovine serum albumin and Rabbit Anti-Mouse Antibody. The nanodiamond-IgG(I125) and RAM-nanodiamond-BSA(I125) complexes are stable in blood serum and the immobilized proteins retain their biological activity. It was shown that the RAM-nanodiamond-BSA(I125) complex is able to bind to the target antigen immobilized on the Sepharose 6B matrix through antibody-antigen interaction. The idea can be extended to use nanodiamonds as carriers for delivery of bioactive substances (i.e., drugs) to various targets in vivo.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mogilnaya O.A., Puzyr A.P., Baron A.V., Bondar V.S.
Заглавие : Hematological Parameters and the State of Liver Cells of Rats After Oral Administration of Aflatoxin B1 Alone and Together with Nanodiamonds
Колич.характеристики :5 с
Коллективы :
Место публикации : Nanoscale Res. Lett.: SPRINGER, 2010. - Vol. 5, Is. 5. - С. 908-912. - ISSN 1931-7573, DOI 10.1007/s11671-010-9571-8
Примечания : Cited References: 23. - The study was financially supported by the Russian Foundation for Basic Research (RFBR) (Grant No. 06-0490234) and the RAS Presidium (Program No. 27, Project No. 64).
Предметные рубрики: B-1
MYCOTOXINS
ADSORPTION
Ключевые слова (''Своб.индексиров.''): nanodiamonds--aflatoxin b(1)--detoxification--adsorbent
Аннотация: Hematological parameters and the state of liver cells of rats were examined in vivo after the animals received aflatoxin B(1) (AfB(1)) alone and together with modified nanodiamonds (MND) synthesized by detonation. The rats that had received the MND hydrosol had elevated leukocyte levels, mainly due to higher granulocyte counts and somewhat increased monocyte counts compared to control rats. Hematological parameters of the rats that had received AfB(1) alone differed from those of the control rats in another way: total white blood cell counts were significantly lower due to the decreased lymphocyte counts. In rats that had consumed AfB(1) with the MND hydrosol, changes in hematological parameters were less pronounced than in rats that had consumed either AfB(1) or MND. Electron microscopy showed that hepatocytes of the rats that had received the MND hydrosol or AfB(1) with the MND hydrosol contained elevated levels of lipid inclusions and lysosomes. Hyperplasia of the smooth endoplasmic reticulum (EPR) was revealed in liver specimens of the rats that had received AfB(1). Results of the study suggest the conclusion about mutual mitigation of the effects of nanoparticles and the mycotoxin on rats blood and liver cells after AfB(1) has adsorbed on MND.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mogilnaya O.A., Puzyr' A.P., Bondar' V.S.
Заглавие : Growth and bioluminescence of luminous bacteria under the action of aflatoxin B1 before and after its treatment with nanodiamonds
Колич.характеристики :5 с
Коллективы :
Место публикации : Appl. Biochem. Microbiol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2010. - Vol. 46, Is. 1. - С. 33-37. - ISSN 0003-6838, DOI 10.1134/S0003683810010059
Примечания : Cited References: 24. - This work was supported by a grant of the Russian Foundation for Basic Research and the National Scientific Fund of the United States no. 06-04-90234.
Предметные рубрики: B-1
MYCOTOXINS
ADSORPTION
FOOD
Аннотация: The effect of aflatoxin B1 on growth and luminescence of marine luminous bacteria P. phosphoreum and recombinant E. coli Z905 cells was investigated. The bidirectional effect of aflatoxin B1 on the studied bacterial species was detected-an inhibition of luminescence in P. phosphoreum and its stimulation in E. coli. It was shown that aflatoxin B1 influences the cell luminescence in the freshly grown cultures and bacteria restored after lyophilization. It was detected that the effect of aflatoxin B1 was graded after interaction with the modified nanodiamond (MND) of detonation synthesis. After mycotoxin's treatment with MND, it does not cause significant changes in bacterial luminescence. The possibilities for the use of P. phosphoreum and E. coli bacteria in the bioluminescent monitoring of aflatoxin B1 and the use of MND for mycotoxin deactivation are discussed.
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