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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Гительзон И.И., Сандалова Т.П.
Заглавие : Перспективы применения биолюминесцентных методов в медицине : научное издание
Место публикации : Вестн. АМН СССР. - 1990. - N 9. - С. 31-35. - ISSN 0002-3027
ГРНТИ : 34.17.09
Предметные рубрики: БИОЛЮМИНЕСЦЕНЦИЯ
ПРИМЕНЕНИЕ
МЕДИЦИНА
BIOLUMINESCENCE
APPLICATION IN MEDICINE
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Барцев С.И., Гительзон И.И.
Заглавие : К вопросу о временной организации бактериальной люминесценции : научное издание
Место публикации : Stud. biophys. - 1985. - Т. 105, N 3. - С. 149-156. - ISSN 0081-6337
ГРНТИ : 34.17.09
Предметные рубрики: БИОЛЮМИНЕСЦЕНЦИЯ
МЕХАНИЗМЫ
МАТЕМАТИЧЕСКИЕ МОДЕЛИ
БАКТЕРИИ
BACTERIAS
BIOLUMINESCENCE
MATHEMATICAL MODELS
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Высоцкий Е.С., Бондарь В.С., Летунов В.Н.
Заглавие : Выделение и очистка Ca-зависимого фотопротеина - обелина из гидроидных полипов Obelia longissima : научное издание
Место публикации : Биохимия. - 1989. - Т. 54, N 6. - С. 965-973. - ISSN 0320-9725
ГРНТИ : 31.23.27
Предметные рубрики: ФОТОПРОТЕИН
КАЛЬЦИЙ-ЗАВИСИМЫЙ
ОБЕЛИН
ВЫДЕЛЕНИЕ
ОЧИСТКА
OBELIA LONGISSIMA
ПОЛИПЫ ГИДРОИДНЫЕ
OBELIN
CA-ACTIVATED PHOTOPROTEIN
EXTRACTION/PURIFICATION
HYDROID POLYPS
BIOLUMINESCENCE
Аннотация: Описан способ выделения и очистки Ca-зависимого фотопротеина - обелина из гидроидных полипов Obelia longissima, включающий: разрушение материала в гипотонич. буфере, фракционирование ПЭГ 6000, фракционирование (NH[4])[2]SO[4] в диапазоне 40-75% насыщения, хроматографию на ДЭАЭ-целлюлозе DE-52, хроматографию на фенил-сефарозе 4В, гель-фильтрацию на сефадексе G-75, гель-фильтрацию на колонке Superose 12 HR 10/30 при помощи системы FPLC. С помощью предложенного метода получен практически чистый обелин с выходом 30-40%. Мол. м. полученного белка, определенная гель-фильтрацией в неденатурирующих условиях, составляет 39,6 кДа, тогда как электрофорез в полиакриламидном геле с додецилсульфатом натрия показывал наличие двух белков с мол. м. 27 и 15,6 кДа соответственно. Библ. 28. Ин-т биофизики СО АН СССР, Красноярск, СССР
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Frank L.A., Borisova V.V., Markova S.V., Malikova N.P., Stepanyuk G.A., Vysotski E.S.
Заглавие : Violet and greenish photoprotein obelin mutants for reporter applications in dual-color assay
Колич.характеристики :6 с
Место публикации : Anal. Bioanal. Chem.: SPRINGER HEIDELBERG, 2008. - Vol. 391, Is. 8. - С. 2891-2896. - ISSN 1618-2642, DOI 10.1007/s00216-008-2223-5
Примечания : Cited References: 22
Предметные рубрики: ANGSTROM RESOLUTION
RECOMBINANT OBELIN
CRYSTAL-STRUCTURE
BIOLUMINESCENCE
AEQUORIN
IMMUNOASSAY
EXPRESSION
CDNA
PURIFICATION
CLONING
Ключевые слова (''Своб.индексиров.''): ca(2+)-regulated photoprotein--bioluminescence--dual-color assay
Аннотация: Two kinds of Ca(2+)-regulated photoprotein obelin with altered color of bioluminescence were obtained by active-center amino acid substitution. The mutant W92F-H22E emits violet light (lambda(max)=390 nm) and the mutant Y139F emits greenish light (lambda (max)=498 nm), with small spectral overlap, both display high activity and stability and thus may be used as reporters. For demonstration, the mutants were applied in dual-color simultaneous immunoassay of two gonadotropic hormones-follicle-stimulating hormone and luteinizing hormone. Bioluminescence of the reporters was simultaneously triggered by single injection of Ca(2+) solution, divided using band-pass optical filters and measured with a two-channel photometer. The sensitivity of simultaneous bioluminescence assay was close to that of a separate radioimmunoassay.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Burakova, Ludmila P., Lyakhovich, Maria S., Mineev, Konstantin S., Petushkov, Valentin N., Zagitova, Renata, I, Tsarkova, Aleksandra S., Kovalchuk, Sergey, I, Yampolsky, Ilia, V, Vysotski, Eugene S., Kaskova, Zinaida M.
Заглавие : Unexpected Coelenterazine Degradation Products of Beroe abyssicola Photoprotein Photoinactivation
Колич.характеристики :4 с
Коллективы : Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [20-04-00085]; Russian Foundation for Basic Research, Krasnoyarsk Territory [20-44-242003]; Krasnoyarsk Regional Fund of Science in part of purification and spectral characterization of native compounds; Russian Science FoundationRussian Science Foundation (RSF) [17-1401169p]; Russian FederationRussian Federation [LS-2605.2020.4]
Место публикации : Org. Lett.: AMER CHEMICAL SOC, 2021. - Vol. 23, Is. 17. - С. 6846-6849. - ISSN 1523-7060, DOI 10.1021/acs.orglett.1c02410. - ISSN 1523-7052(eISSN)
Примечания : Cited References:20. - This work was supported by grant 20-04-00085 of the Russian Foundation for Basic Research, grant 20-44-242003 of the Russian Foundation for Basic Research, Krasnoyarsk Territory, and Krasnoyarsk Regional Fund of Science in part of purification and spectral characterization of native compounds, grant 17-1401169p of the Russian Science Foundation, and the President of Russian Federation grant for Leading Scientific Schools LS-2605.2020.4 in part of structural elucidation of native products and organic synthesis. We thank Konstantin Antonov (IBCh RAS) and Igor Ivanov (IBCh RAS) for the registration of HRMS spectra.
Предметные рубрики: CRYSTAL-STRUCTURE
BIOLUMINESCENCE
OBELIN
RESIDUES
BINDING
Аннотация: Ca2+-regulated photoproteins of ctenophores lose bioluminescence activity when exposed to visible light. Little is known about the chemical nature of chromophore photo-inactivation. Using a total synthesis strategy, we have established the structures of two unusual coelenterazine products, isolated from recombinant berovin of the ctenophore Beroe abyssicola, which are Z/E isomers. We propose that during light irradiation, these derivatives are formed from 2-hydroperoxycoelenterazine via the intermediate 8a-peroxide by a mechanism reminiscent of that previously described for the auto-oxidation of green-fluorescent-protein-like chromophores.
WOS
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mogil'naya, O. A., Ronzhin, N. O., Medvedeva, S. E., Bondar', V. S.
Заглавие : Total peroxidase and catalase activity of luminous basidiomycetes Armillaria borealis and Neonothopanus nambi in comparison with the level of light emission
Колич.характеристики :6 с
Коллективы : Siberian Branch of the Russian Academy of Sciences [71]
Место публикации : Appl. Biochem. Microbiol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2015. - Vol. 51, Is. 4. - С. 419-424. - ISSN 0003-6838, DOI 10.1134/S0003683815040110. - ISSN 1573-8183(eISSN)
Примечания : Cited References:35. - The authors are grateful to N. V. Psurtseva (curator of the collection of basidiomycetes of the Botanical Institute, Russian Academy of Science) for help with the species affiliation of the IBSO 2328 culture. This work was supported by the Program of Interdisciplinary Projects of the Siberian Branch of the Russian Academy of Sciences, project no. 71.
Предметные рубрики: OXIDATIVE STRESS
SYSTEM
FUNGI
BIOLUMINESCENCE
LUMINESCENCE
Ключевые слова (''Своб.индексиров.''): basidiomycetes--luminescence--peroxidase--catalase
Аннотация: The peroxidase and catalase activities in the mycelium of luminous basidiomycetes Armillaria borealis and Neonothopanus nambi in normal conditions and under stress were compared. An increase in the luminescence level was observed under stress, as well as an increase in peroxidase and catalase activities. Moreover, the peroxidase activity in extracts of A. borealis mycelium was found to be almost one and a half orders of magnitude lower, and the catalase activity more than two orders of magnitude higher in comparison with the N. nambi mycelium. It can be suggested that the difference between the brightly luminescent and dimly luminescent mycelium of N. nambi is due to the content of (HO2)-O-2 or other peroxide compounds.
WOS,
Scopus
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Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Eremeeva E.V., Markova S.V., Frank L.A., Vysotski E.S.
Заглавие : THE MAIN FUNCTION OF HIS175, TRP179, AND TYR190 RESIDUES OF THE OBELIN BINDING SITE IS TO STABILIZE THE HYDROPEROXYCOELENTERAZINE INTERMEDIATE
Колич.характеристики :4 с
Место публикации : BIOLUMINESCENCE AND CHEMILUMINESCENCE: CHEMISTRY, BIOLOGY AND APPLICATIONS: WORLD SCIENTIFIC PUBL CO PTE LTD, 2007. - 14th International Symposium on Bioluminescence and Chemiluminescence (OCT 15-19, 2006, San Diego, CA). - С. 7-10. - ISBN 978-981-270-816-8, DOI 10.1142/9789812770196_0002
Примечания : Cited References: 7
Предметные рубрики: PHOTOPROTEIN OBELIN
BIOLUMINESCENCE
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Krasitskaya, Vasilisa V., Goncharova, Natalia S., Biriukov, Vladislav V., Bashmakova, Eugenia E., Kabilov, Marsel R., Baykov, Ivan K., Sokolov, Aleksey E., Frank, Ludmila A.
Заглавие : The Ca2+-Regulated Photoprotein Obelin as a Tool for SELEX Monitoring and DNA Aptamer Affinity Evaluation
Колич.характеристики :6 с
Коллективы : Russian Foundation for Basic Research (RFBR)Russian Foundation for Basic Research (RFBR) [18-38-00531]
Место публикации : Photochem. Photobiol.: WILEY, 2020. - Article in press. - ISSN 0031-8655, DOI 10.1111/php.13274. - ISSN 1751-1097(eISSN)
Примечания : Cited References:25. - This work has been supported by the Russian Foundation for Basic Research (RFBR) under the grant no 18-38-00531.
Предметные рубрики: CARDIAC TROPONIN-I
BIOLUMINESCENCE
IMMUNOASSAY
APTASENSOR
DIAGNOSIS
Аннотация: Bioluminescent solid-phase analysis was proposed to monitor the selection process and to determine binding characteristics of the aptamer-target complexes during design and development of the specific aptamers. The assay involves Ca2+-regulated photoprotein obelin as a simple, sensitive and fast reporter. Applicability and the prospects of the approach were exemplified by identification of DNA aptamers to cardiac troponin I, a highly specific early biomarker for acute myocardial infarction. Two structurally different aptamers specific to various epitopes of troponin I were obtained and then tested in a model bioluminescent assay.
WOS
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Stepanyuk G.A., Liu Z.J., Vysotski E.S., Lee J..., Rose J.P., Wang B.C.
Заглавие : Structure based mechanism of the Ca2+ -induced release of coelenterazine from the Renilla binding protein
Колич.характеристики :11 с
Место публикации : Proteins: WILEY-BLACKWELL, 2009. - Vol. 74, Is. 3. - С. 583-593. - ISSN 0887-3585, DOI 10.1002/prot.22173
Примечания : Cited References: 26
Предметные рубрики: GREEN-FLUORESCENT PROTEIN
CRYSTAL-STRUCTURES
RENIFORMIS
LUCIFERASE
BIOLUMINESCENCE
PURIFICATION
ANGSTROM
MUELLERI
Ключевые слова (''Своб.индексиров.''): bioluminescence--ef-hand--coelenteramider--luciferase--ca2+-binding protein
Аннотация: The crystal structure of the Ca2+-loaded coelenterazine binding protein from Renilla muelleri in its apo-state has been determined at resolution 1.8 angstrom. Although calcium binding hardly affects the compact scaffold and overall fold of the structure before calcium addition, there are easily discerned shifts in the residues that were interacting with the coelenterazine and a repositioning of helices, to expose a cavity to the external solvent. Altogether these changes offer a straightforward explanation for how following the addition of Ca2+, the coelenterazine could escape and become available for bioluminescence on Renilla luciferase. A docking computation supports the possibility of a luciferase-binding protein complex.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kobzeva T.V., Melnikov A.R., Karogodina T.Y., Zikirin S.B., Stass D.V., Molin Y.N., Rodicheva E.K., Medvedeva S.E., Puzyr A.P., Burov A.A., Bondar V.S., Gitelson J.I.
Заглавие : Stimulation of luminescence of mycelium of luminous fungus Neonothopanus nambi by ionizing radiation
Колич.характеристики :8 с
Коллективы : Program of Siberian Branch of Russian Academy of Sciences [71]; Council for Grants of the President of the Russian Federation for Support of Leading Scientific Schools [NSh 2272.2012.3]; Russian Foundation for Basic Research [12-03-33082]; Program of Government of Russian Federation "On the Efforts for Attracting Leading Researchers to Educational Institutions of Russia" [11.G34.31.0058]
Место публикации : Luminescence: WILEY-BLACKWELL, 2014. - Vol. 29, Is. 7. - С. 703-710. - ISSN 1522-7235, DOI 10.1002/bio.2656. - ISSN 1522-7243
Примечания : Cited References: 29. - The work was supported by the Program of Siberian Branch of Russian Academy of Sciences (project no. 71), Council for Grants of the President of the Russian Federation for Support of Leading Scientific Schools (project no. NSh 2272.2012.3), the Russian Foundation for Basic Research (project no. 12-03-33082), and the Program of Government of Russian Federation "On the Efforts for Attracting Leading Researchers to Educational Institutions of Russia" (grant no. 11.G34.31.0058).
Предметные рубрики: BIOLUMINESCENCE
COMPONENTS
MECHANISMS
SYSTEM
Ключевые слова (''Своб.индексиров.''): higher luminous fungi--neonothopanus nambi--ionizing irradiation--reactive oxygen species--lipid peroxidation
Аннотация: The luminescent system of higher luminous fungi is not fully understood and the enzyme/substrate pair of the light emission reaction has not been isolated. It was suggested that luminescence of fungi involves oxidase-type enzymes, and reactive oxygen species are important for fungal light production. Generation of reactive oxygen species can be stimulated by ionizing irradiation, which has not been studied for luminous fungi. We report the effect of X-irradiation on the luminescence of fungus Neonothopanus nambi. Experiments were performed withmyceliumon a home-built setup based on an X-ray tube and monochromator/photomultiplier tube. Application of X-rays does not change the emission spectrum, but after approximately 20 min of continuous irradiation, light production from unsupported mycelium starts growing and increases up to approximately five times. After peaking, its level decreases irrespective of the presence of X-irradiation. After staying at a certain level, light production collapses to zero, which is not related to the drying of the mycelium or thermal impact of radiation. The observed shape of kinetics is characteristic of a multistage and/or chain reaction. The time profile of light production must reflect the current levels of radicals present in the system and/or the activity of enzyme complexes involved in light production. Copyright (C) 2014 John Wiley & Sons, Ltd.
WOS
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Purtov, K., V, Petushkov V. N., Rodionova N. S., Chepurnykh, T., V, Kozhemyako V. B., Zagitova, R., I, Shcheglov A. S., Ziganshin R. H., Tsarkova A. S.
Заглавие : SIMILARITIES AND DIFFERENCES BETWEEN THE CHAETOPTERUS VARIOPEDATUS POLYCHAETE LUCIFERASES DEPENDING ON THE TYPE OF HABITAT
Колич.характеристики :6 с
Место публикации : Bull. Russ. State Med. Univ.: PIROGOV RUSSIAN NATL RESEARCH MEDICAL UNIV, 2021. - Is. 5. - С. 41-46. - ISSN 2500-1094, DOI 10.24075/vrgmu.2021.049. - ISSN 2542-1204(eISSN)
Примечания : Cited References:20
Предметные рубрики: BIOLUMINESCENCE
ANNELIDA
SYSTEM
Аннотация: The marine polychaete Chaetopterus variopedatus (Renier) (family Chaetopteridae) is a cosmopolitan species complex, consisting of distinct populations/subspecies. The worms release glowing (460 nm) clouds of mucus when disturbed, and their parapodia often glow brightly. Currently, it is still unclear how exactly the bioluminescence system of these polychaetes functions. It has been previously assumed that the C. variopedatus luciferase may be used for detection of ferroptosis, the recently explored pathway of programmed cell death, resulting from accumulation of the ferrous ions. This study was aimed to extract and characterize the C. variopedatus luciferases, as well as to compare luciferases obtained from C. variopedatus of different populations. When extracting the enzyme responsible for bioluminescence from the frozen samples of Brazilian C. variopedatus using the improved method, two active luciferases, L1 and L2, were obtained. We assumed that one of the listed above luciferases was responsible for luminescence of the mucus and the other luciferase was responsible for luminescence in parapodia, and used the method for the distinct samples of mucus and parapodia of the living Far Eastern C. variopedatus. However, mucus of the latter turned out to be non-glowing. It is shown that luciferase L2 is responsible for luminescence in the parapodia of the C. variopedatus polychaete, since this luciferase has been found in the total biomass of Brazilian polychaetes and parapodia of Far Eastern polychaetes. Luminescence of the Brazilian C. variopedatus mucus is attributed to the functioning of luciferase L1, which is lacking in the mucus of the Far Eastern subspecies. The range of luciferase isoforms in polychaetes C. variopedatus depends on the place of origin.
WOS
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Illarionov B.A., Frank L.A., Illarionova V.A., Bondar V.S., Vysotski E.S., Blinks J.R.
Заглавие : Recombinant obelin: Cloning and expression of cDNA, purification, and characterization as a calcium indicator
Колич.характеристики :27 с
Место публикации : Methods Enzymol.: ACADEMIC PRESS INC, 2000. - Vol. 305. - С. 223-249. - ISSN 0076-6879
Примечания : Cited References: 58
Предметные рубрики: PHOTOPROTEIN OBELIN
MESSENGER-RNA
CA-2+-ACTIVATED PHOTOPROTEIN
DIRECTED MUTAGENESIS
SEQUENCE-ANALYSIS
HYDROID OBELIA
AEQUORIN
PROTEIN
BIOLUMINESCENCE
LUMINESCENCE
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Eremeeva E.V., Natashin P.V., Song L..., Zhou Y.G., van Berkel WJH, Liu Z.J., Vysotski E.S.
Заглавие : Oxygen Activation of Apo-obelin-Coelenterazine Complex
Колич.характеристики :7 с
Место публикации : ChemBioChem: WILEY-V C H VERLAG GMBH, 2013. - Vol. 14, Is. 6. - С. 739-745. - ISSN 1439-4227, DOI 10.1002/cbic.201300002
Примечания : Cited References: 46. - The work was supported by grants from the RFBR 12-04-91153, and NSFC 31270795 and 31021062, by the Russian Federation Government Program "Measures to Attract Leading Scientists to Russian Educational Institutions" (grant 11.G34.31.0058), "Molecular and Cellular Biology" of RAS, President of the Russian Federation "Leading Science School" (grant 1044.2012.2). E.V.E. was supported by a Wageningen University Sandwich PhD Fellowship Program.
Предметные рубрики: GREEN-FLUORESCENT PROTEIN
JELLYFISH CLYTIA-GREGARIA
CRYSTAL-STRUCTURE
CA2+-DISCHARGED PHOTOPROTEIN
ANGSTROM RESOLUTION
RECOMBINANT OBELIN
MOLECULAR-OXYGEN
AEQUORIN
CALCIUM
BIOLUMINESCENCE
Ключевые слова (''Своб.индексиров.''): aequorin--coelenterazine--luminescence--photoprotein--protein folding
Аннотация: Ca2+-regulated photoproteins use a noncovalently bound 2-hydroperoxycoelenterazine ligand to emit light in response to Ca2+ binding. To better understand the mechanism of formation of active photoprotein from apoprotein, coelenterazine and molecular oxygen, we investigated the spectral properties of the anaerobic apo-obelincoelenterazine complex and the kinetics of its conversion into active photoprotein after exposure to air. Our studies suggest that coelenterazine bound within the anaerobic complex might be a mixture of N7-protonated and C2() anionic forms, and that oxygen shifts the equilibrium in favor of the C2() anion as a result of peroxy anion formation. Proton removal from N7 and further protonation of peroxy anion and the resulting formation of 2-hydroperoxycoelenterazine in obelin might occur with the assistance of His175. It is proposed that this conserved His residue might play a key role both in formation of active photoprotein and in Ca2+-triggering of the bioluminescence reaction.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A..., Kalacheva G.S., Medvedeva S..., Rodicheva E..., Volova T.G.
Заглавие : Luminous bacteria as producers of polyhydroxyalkanoates
Место публикации : Macromol. Symp.: WILEY-V C H VERLAG GMBH, 2008. - Vol. 269: 4th European Symposium on Biopolymers (OCT 02-04, 2007, Kusadasi, TURKEY). - С. 17-22. - 6. - ISSN 1022-1360, DOI 10.1002/masy.200850904
Примечания : Cited References: 16
Предметные рубрики: VIBRIO-HARVEYI
BIOLUMINESCENCE
Ключевые слова (''Своб.индексиров.''): luminous bacteria--polyhydroxyalkanoates--polyhydroxybutyrate
Аннотация: The study addresses the ability of luminous bacteria of different taxa (Photobacterium leiognathi, Photobacterium phosphoreum, Vibrio harveyi, Vibrio fischeri) to synthesize polyesters of hydrocarbon acids (polyhydroxyalkanoates, PHAs) as storage macromolecules. The screened strains widely varied in their PHA productivity. Conditions for attaining high polymer yields (including two- and three-component polymers) in batch culture have been determined. The attained polymer yields reached 40-70% of dry cell biomass. The results suggest a conclusion that luminous microorganisms can be considered as producers of multi-component PHAs.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : VYDRYAKOVA G.A., KUZNETSOV A.M., PRIMAKOVA G.A., CHUGAEVA Y.V., FISH A.M.
Заглавие : LUMINESCENT BACTERIAL SYMBIONTS AND COMMENSALS OF LUMINESCENT AND NONLUMINESCENT MARINE ANIMALS OF THE INDIAN-OCEAN
Колич.характеристики :4 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1995. - Vol. 64, Is. 5. - P589-592. - ISSN 0026-2617
Примечания : Cited References: 18
Предметные рубрики: BIOLUMINESCENCE
SEAWATER
Аннотация: Approximately 100 fish belonging to 24 families and several representatives of cephalopods, prawns, and euphausiids were investigated for the presence of luminescent bacteria. Species identification of isolated luminescent bacteria was performed, and the frequency and ratio of their occurrence in the gastrointestinal microflora of marine animals were determined. Luminescent bacteria occurred in 23 - 65% of the fish, depending on the habitat depth, and their ratio varied from 8 to 60% of the total gastrointestinal microflora of fish. The free-living luminescent bacteria were found in 50% of the seawater samples from depths down to 1000 m. The luminescent bacterium Photobacterium phosphoreum was dominant among the isolated cultures.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Eremeeva E.V., Vysotski E.S., Westphal A.H., van Mierlo CPM, van Berkel WJH
Заглавие : Ligand binding and conformational states of the photoprotein obelin
Колич.характеристики :7 с
Место публикации : FEBS Lett.: ELSEVIER SCIENCE BV, 2012. - Vol. 586, Is. 23. - С. 4173-4179. - ISSN 0014-5793, DOI 10.1016/j.febslet.2012.10.015
Примечания : Cited References: 24. - The work was supported by RFBR grant 12-04-00131, by the Program of the Government of Russian Federation "Measures to Attract Leading Scientists to Russian Educational Institutions" (grant 11.G34.31.058), by the Program "Molecular and Cellular Biology" of RAS. The Wageningen University Sandwich PhD-Fellowship Program supported E.V.E.
Предметные рубрики: RECOMBINANT OBELIN
CRYSTAL-STRUCTURE
LIGHT-EMISSION
APO-AEQUORIN
BIOLUMINESCENCE
COELENTERAZINE
LUMINESCENCE
STABILITY
ANGSTROM
PROTEINS
Ключевые слова (''Своб.индексиров.''): bioluminescence--coelenterazine--photoprotein--thermostability
Аннотация: Many proteins require a non-covalently bound ligand to be functional. How ligand binding affects protein conformation is often unknown. Here we address thermal unfolding of the free and ligand-bound forms of photoprotein obelin. Fluorescence and far-UV circular dichroism ( CD) data show that the various ligand-dependent conformational states of obelin differ significantly in stability against thermal unfolding. Binding of coelenterazine and calcium considerably stabilizes obelin. In solution, all obelin structures are similar, except for apo-obelin without calcium. This latter protein is an ensemble of conformational states, the populations of which alter upon increasing temperature. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : SANDALOVA T.P., TYULKOVA N.A.
Заглавие : INACTIVATION OF BACTERIAL LUCIFERASES BY N-ETHYLMALEIMIDE
Колич.характеристики :7 с
Место публикации : Biochem.-Moscow: PLENUM PUBL CORP, 1992. - Vol. 57, Is. 6. - P552-558. - ISSN 0006-2979
Примечания : Cited References: 21
Предметные рубрики: AMINO-ACID SEQUENCE
NUCLEOTIDE-SEQUENCE
REACTIVE SULFHYDRYL
PHOTOBACTERIUM-LEIOGNATHI
VIBRIO-HARVEYI
BIOLUMINESCENCE
SUBUNIT
REGION
GENE
Ключевые слова (''Своб.индексиров.''): luciferase--n-ethylmaleimide
Аннотация: The kinetics of inactivation of luciferases from four species of luminescent bacteria by the thiol reagent N-ethylmaleimide were investigated The dependencies of inactivation on ionic strength differed among the enzymes. Increasing the molarity of the buffer increased the rate of inactivation of all luciferases except that of Vibrio harveyi. Modification of Photobacterium phosphoreum luciferase decreased the maximal intensity of bioluminescence, whereas modification of Photobacterium leiognathi and Vibrio fischeri luciferases in high ionic strength buffers decreased the maximal intensity of bioluminescence and changed the luminescence decay rate constant. High ionic strength apparently alters the conformational states of the luciferases.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Eremeeva E.V., Markova S.V., Vysotski E.S.
Заглавие : Highly active BRET-reporter based on yellow mutant of Renilla muelleri luciferase
Колич.характеристики :4 с
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2013. - Vol. 450, Is. 1. - С. 147-150. - ISSN 1607-6729, DOI 10.1134/S1607672913030095
Примечания : Cited References: 14. - This work was supported by the Ministry of Education and Science of the Russian Federation (Government Contract no. 16.512.11.2141) and Council of the President of the Russian Federation on Grants and State Support of Leading Scientific Schools (project no. NSh-64987.2010.4).
Предметные рубрики: GREEN-FLUORESCENT PROTEIN
GENE-EXPRESSION
CDNA
CLONING
BIOLUMINESCENCE
RENIFORMIS
Scopus
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Markova S.V., Burakova L.P., Vysotski E.S.
Заглавие : High-active truncated luciferase of copepod Metridia longa
Колич.характеристики :6 с
Место публикации : Biochem. Biophys. Res. Commun.: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2012. - Vol. 417, Is. 1. - С. 98-103. - ISSN 0006-291X, DOI 10.1016/j.bbrc.2011.11.063
Примечания : Cited References: 31. - This study was supported by the Grants 16.512.11.2141 and 64987.2010.4 of the Ministry of Education and Science of Russian Federation.
Предметные рубрики: COELENTERAZINE-BINDING PROTEIN
REPORTER-GENE-EXPRESSION
RENILLA LUCIFERASE
GAUSSIA LUCIFERASE
LIGHT-EMITTER
IN-VIVO
BIOLUMINESCENCE
PHOTOPROTEINS
CDNA
SUBSTRATE
Ключевые слова (''Своб.индексиров.''): bioluminescence--coelenterazine--mammalian expression--secretion
Аннотация: The technology of real-time imaging in living cells is crucial for understanding of intracellular events. For this purpose, bioluminescent reporters have been introduced as sensitive and convenient tools. Metridia luciferase (MLuc) from the copepod Metridia longa is a coelenterazine-dependent luciferase containing a natural signal peptide for secretion. We report the high-active MLuc mutants with deletion of the N-terminal variable part of amino acid sequence. The MLuc variants were produced in Escherichia coil cells, converted to an active protein, and characterized. We demonstrate that the truncated MLucs have significantly increased bioluminescent activity as against the wild type enzyme but substantially retain other properties. One of the truncated variants of MLuc was transiently expressed in HEK 293 cells. The results clearly suggest that the truncated Metridia luciferase is well suited as a secreted reporter ensuring higher detection sensitivity in comparison with a wild type enzyme. (C) 2011 Elsevier Inc. All rights reserved.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Medvedeva S. E., Artemenko K. S., Krivosheenko A. A., Rusinova A. G., Rodicheva E. K., Puzyr A. P., Bondar V. S.
Заглавие : Growth and light emission of luminous basidiomycetes cultivated on solid media and in submerged culture
Колич.характеристики :13 с
Коллективы : RF Government [11.G34.31.058]; SB RAS [71, 38]
Место публикации : Mycosphere: MYCOSPHERE PRESS, 2014. - Vol. 5, Is. 4. - С. 565-577. - ISSN 2077-7000, DOI 10.5943/mycosphere/5/4/9
Примечания : Cited References:23. - This study was supported by grant No. 11.G34.31.058 (RF Government) and Projects No. 71 and No. 38 (SB RAS).
Предметные рубрики: MYCELIAL GROWTH
PANELLUS-STYPTICUS
BIOLUMINESCENCE
LUMINESCENCE
Ключевые слова (''Своб.индексиров.''): luminescence--luminous higher fungi--mycelium
Аннотация: There are higher fungi that emit visible light; however, little is known about their requirements for good growth and bright luminescence. Knowledge of these requirements is extremely important for maintaining fungal cultures in laboratory conditions and preparation of luminous mycelia for research purposes. Luminous higher fungi Panellus stipticus, Armillaria sp. and Neonothopanus nambi isolated from different climatic areas and maintained in CCIBSO 836 (Collection of IBP SB RAS, Russia) were used for experiments. Techniques for static and submerged cultivation of mycelia of higher fungi have been developed and optimized for the production of samples of aerial and globular mycelia with prolonged and stable luminescence. We investigated the growth characteristics and luminescence of mycelia cultivated in/on different nutrient media, and the effects of deionized water and mechanical damage on the light emission of mycelia. An increase in luminescence intensity of fungal mycelia can be obtained during cultivation of fungi on a nutrient medium with a certain composition. A significant increase in light emission from N. nambi mycelium can also be obtained after its incubation in water and mechanical damage. The light emission from N. nambi mycelium was greatly enhanced after these treatments, in contrast to the mycelia of Armillaria sp. or P. stipticus. Cultivation conditions that enable growing mycelia with high levels of luminescence will expedite further studies to gain a better understanding of fungal bioluminescence.
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