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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vysotski E.S., Lee J...
Заглавие : Ca2+-regulated photoproteins: Structural insight into the bioluminescence mechanism
Колич.характеристики :11 с
Место публикации : Accounts Chem. Res.: AMER CHEMICAL SOC, 2004. - Vol. 37, Is. 6. - С. 405-415. - ISSN 0001-4842, DOI 10.1021/ar0400037
Примечания : Cited References: 44
Предметные рубрики: AEQUORIN BIOLUMINESCENCE
SEQUENCE-ANALYSIS
CALCIUM-BINDING
CA2+-BINDING PHOTOPROTEIN
VIOLET BIOLUMINESCENCE
ANGSTROM RESOLUTION
FUNCTIONAL PART
EXCITED-STATES
W92F OBELIN
COELENTERAZINE
Аннотация: The bioluminescent jellyfish has contributed two famous proteins to modern science: green fluorescent protein or GFP, which finds wide use as a probe in cell biology studies, and aequorin, which has been used for intracellular calcium measurement for more than 30 years. More recently, obelin, a protein from the bioluminescent hydroid and also in the family of what are called "Ca2+-regulated photoproteins", has been shown to have very attractive properties both in general applications and for basic structural biology investigations. This review will survey the new information into their molecular mechanism of bioluminescence action.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gealageas R..., Malikova N.P., Picaud S..., Borgdorff A.J., Burakova L.P., Brulet P..., Vysotski E.S., Dodd R.H.
Заглавие : Bioluminescent properties of obelin and aequorin with novel coelenterazine analogues
Колич.характеристики :13 с
Коллективы : ICSN; CNRS Physics, Chemistry and Biology interface grant; RFBR [12-04-00131]; Government of Russian Federation [11.G34.31.0058]; ANR
Место публикации : Anal. Bioanal. Chem.: SPRINGER HEIDELBERG, 2014. - Vol. 406, Is. 11. - С. 2695-2707. - ISSN 1618-2642, DOI 10.1007/s00216-014-7656-4. - ISSN 1618-2650
Примечания : Cited References: 57. - R.G. acknowledges the ICSN for a fellowship. We are grateful for the ANR grant to P.B. and a CNRS Physics, Chemistry and Biology interface grant to R.H.D. and P.B.; N.P.M, L.P.B., and E.S.V. acknowledge the RFBR grant 12-04-00131 and the Program of the Government of Russian Federation "Measures to attract leading scientists to Russian educational institutions" (grant 11.G34.31.0058). P.B. and A.J.B. are indebted to Eric Karplus from Science Wares Inc. for helping with single-photon imaging software.
Предметные рубрики: PHOTOPROTEIN OBELIN
CRYSTAL-STRUCTURE
CA2+-REGULATED PHOTOPROTEINS
CA2+-ACTIVATED PHOTOPROTEIN
SEMISYNTHETIC AEQUORINS
ANGSTROM RESOLUTION
RECOMBINANT OBELIN
BINDING PROTEIN
CALCIUM-BINDING
CA2+ DYNAMICS
Ключевые слова (''Своб.индексиров.''): bioluminescence--luciferase--photoprotein--coelenterazine
Аннотация: The main analytical use of Ca2+-regulated photoproteins from luminous coelenterates is for real-time non-invasive visualization of intracellular calcium concentration ([Ca2+](i)) dynamics in cells and whole organisms. A limitation of this approach for in vivo deep tissue imaging is the fact that blue light emitted by the photoprotein is highly absorbed by tissue. Seven novel coelenterazine analogues were synthesized and their effects on the bioluminescent properties of recombinant obelin from Obelia longissima and aequorin from Aequorea victoria were evaluated. Only analogues having electron-donating groups (m-OCH3 and m-OH) on the C6 phenol moiety or an extended resonance system at the C8 position (1-naphthyl and alpha-styryl analogues) showed a significant red shift of light emission. Of these, only the alpha-styryl analogue displayed a sufficiently high light intensity to allow eventual tissue penetration. The possible suitability of this compound for in vivo assays was corroborated by studies with aequorin which allowed the monitoring of [Ca2+](i) dynamics in cultured CHO cells and in hippocampal brain slices. Thus, the alpha-styryl coelenterazine analogue might be potentially useful for non-invasive, in vivo bioluminescence imaging in deep tissues of small animals.
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