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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : GITELZON I.I., SANDALOVA T.P.
Заглавие : PROSPECTS FOR APPLICATION OF BIOLUMINESCENCE METHOD IN MEDICINE
Колич.характеристики :5 с
Место публикации : VESTNIK AKADEMII MEDITSINSKIKH NAUK SSSR: IZD VO MEDITSINA, 1990. - Is. 9. - С. 31-35. - ISSN 0002-3027
Примечания : Cited References: 41
Предметные рубрики: AMINO-ACID SEQUENCE
NUCLEOTIDE-SEQUENCE
VIBRIO-HARVEYI
BACTERIAL LUCIFERASE
FIREFLY LUCIFERASE
SUBUNIT
CELLS
GENE
PHOTOPROTEINS
EXPRESSION
Аннотация: Major advances in the development and application of the bioluminescent analysis to detect certain biologically active substances are discussed. The main merit of the method lies in its high sensitivity and specificity along with its simplicity and rapid performance. The available methodologies allow for detection of substances of varying nature: Ca2+, ATP, FMN, NAD(P), long-chain aldehydes, ATP- and NAD(P)-dependent enzymes and their substrates, many xenobiotics and antibiotics, and mutagens. The bioluminescence methodologies may be widely applied in clinical laboratory diagnosis.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Eke G..., Kuzmina A.M., Goreva A.V., Shishatskaya E.I., Hasirci N..., Hasirci V...
Заглавие : In vitro and transdermal penetration of PHBV micro/nanoparticles
Колич.характеристики :11 с
Коллективы : Government of the Russian Federation [220, 11.G34.31.0013, MD-3112.2012.4]; EC FP7 SKINTREAT project; State Planning Organization (Turkey)
Место публикации : J. Mater. Sci.-Mater. Med.: SPRINGER, 2014. - Vol. 25, Is. 6. - С. 1471-1481. - ISSN 0957-4530, DOI 10.1007/s10856-014-5169-5. - ISSN 1573-4838
Примечания : Cited References: 38. - The study was supported by the Government of the Russian Federation (Decree No. 220 of 09.04.2010) (Agreement No. 11.G34.31.0013) and (Grant No MD-3112.2012.4). We gratefully acknowledge the EC FP7 SKINTREAT project and the State Planning Organization (Turkey) for the grant to establish BIOMATEN. Mr. A. Buyuksungur is acknowledged for his contributions with CLSM.
Предметные рубрики: DRUG-DELIVERY
PLGA NANOPARTICLES
CELLULAR UPTAKE
MICROPARTICLES
POLYHYDROXYALKANOATES
CYTOTOXICITY
SIZE
POLYESTERS
MECHANISM
CELLS
Аннотация: The purpose of this study was to develop micro and nano sized drug carriers from poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), and study the cell and skin penetration of these particles. PHBV micro/nanospheres were prepared by o/w emulsion method and were stained with a fluorescent dye, Nile Red. The particles were fractionated by centrifugation to produce different sized populations. Topography was studied by SEM and average particle size and its distribution were determined with particle sizer. Cell viability assay (MTT) was carried out using L929 fibroblastic cell line, and particle penetration into the cells were studied. Transdermal permeation of PHBV micro/nanospheres and tissue reaction were studied using a BALB/c mouse model. Skin response was evaluated histologically and amount of PHBV in skin was determined by gas chromatography-mass spectrometry. The average diameters of the PHBV micro/nanosphere batches were found to be 1.9 mu m, 426 and 166 nm. Polydispersity indices showed that the size distribution of micro sized particles was broader than the smaller ones. In vitro studies showed that the cells had a normal growth trend. MTT showed no signs of particle toxicity. The 426 and 166 nm sized PHBV spheres were seen to penetrate the cell membrane. The histological sections revealed no adverse effects. In view of this data nano and micro sized PHBV particles appeared to have potential to serve as topical and transdermal drug delivery carriers for use on aged or damaged skin or in cases of skin diseases such as psoriasis, and may even be used in gene transfer to cells.
WOS
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : PARAMONOV L.E.
Заглавие : A THEORETICAL-ANALYSIS OF OPTICAL-ABSORPTION SPECTRA OF ALGAE
Колич.характеристики :6 с
Место публикации : Okeanologiya: MEZHDUNARODNAYA KNIGA, 1995. - Vol. 35, Is. 5. - P719-724. - ISSN 0030-1574
Примечания : Cited References: 30
Предметные рубрики: NATURAL-WATERS
PHYTOPLANKTON PIGMENTS
LIGHT
ATTENUATION
CELLS
CHLOROPHYLL
SCATTERING
COASTAL
Аннотация: The formula for the absorption cross sections of macroscopically isotropic volume element which consists of ''soft'' particles is proposed. The transformation formula for absorption cross sections on disperse composition is given. The dependence of absorption spectra of green, blue-green and diatom algae on microstructure of suspension is considered. Some inverse problems are discussed.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vinokurov A.P., Eremeev A.V., Setkov N.A.
Заглавие : Dobutamine prevents experimental postintoxication liver cirrhosis in mice
Колич.характеристики :4 с
Место публикации : Bull. Exp. Biol. Med.: CONSULTANTS BUREAU, 2002. - Vol. 134, Is. 1. - P43-46. - ISSN 0007-4888, DOI 10.1023/A:1020604621142
Примечания : Cited References: 14
Предметные рубрики: GROWTH-FACTOR-BETA
HEPATIC-FIBROSIS
RAT HEPATOCYTES
NOREPINEPHRINE
INJURY
CELLS
Ключевые слова (''Своб.индексиров.''): hepatocytes--dobutamine--proliferation--liver cirrhosis
Аннотация: Various chronic inflammatory and necrotic processes in the liver parenchyma are accompanied by pathological morphofunctional changes, which are associated with hepatocyte death and, hyperplasia of the connective tissue. Regeneration of the liver parenchyma should include not only prevention of fibrosis, but also stimulation of hepatocyte proliferation. The adrenoceptor agonist dobutamine stimulated proliferative activity of cultured hepatocytes and prevented the development of postintoxication liver cirrhosis in mice produced by chronic poisoning with CCl4.
WOS
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bolsunovsky, A. Ya., Dementyev D. V., Trofimova E. A., Iniatkina E. M., Kladko, Yu. V., Petrichenkov M. V.
Заглавие : Cytogenetic Effects of -Radiation in Onion (Allium cepa L.) Seedlings
Колич.характеристики :5 с
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2018. - Vol. 481, Is. 1. - С. 181-185. - ISSN 1607-6729, DOI 10.1134/S1607672918040014. - ISSN 1608-3091(eISSN)
Примечания : Cited References:15
Предметные рубрики: YENISEI RIVER
CELLS
Аннотация: The effect of -radiation on the cytogenetic parameters of root meristem cells of onion seedlings was studied in laboratory experiments (Allium-test). An increase in the overall frequency of chromosomal aberrations and micronucleus frequencies in seedling cells at low -radiation doses (0.1 Gy) was detected for the first time. At a maximum absorbed dose of 13 Gy, chromosomal aberrations were detected in the majority of cells in the anaphase and telophase stages of the cell cycle, and the number of cells with multiple aberrations increased. The main contribution to the overall frequency of chromosomal aberrations, in addition to multiple aberrations, is made by the bridge-type aberrations, fragments, and lagging chromosomes. The data obtained allow using the cytogenetic indices of Allium cepa seedlings to assess the biological effects of lowdose -radiation.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bolsunovsky, A. Ya., Dementyev D. V., Frolova T. S., Trofimova E. A., Iniatkina E. M., Vasilyev S. A., Sinitsyna O. I.
Заглавие : Effects of Gamma-Radiation on DNA Damage in Onion (Allium cepa L.) Seedlings
Колич.характеристики :5 с
Коллективы : Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [18-44-240001]; Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2019. - Vol. 489, Is. 1. - С. 362-366. - ISSN 1607-6729, DOI 10.1134/S1607672919060024. - ISSN 1608-3091(eISSN)
Примечания : Cited References:14. - Work on assessing DNA damage in onion seedling nuclei was performed using the equipment of the Core Facility for Microscopic Analysis of Biological Objects, Siberian Branch, Russian Academy of Sciences, funded under the research at the Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences. This work was supported in part by the Russian Foundation for Basic Research (project no. 18-44-240001).
Предметные рубрики: COMET ASSAY
REPAIR
CELLS
Аннотация: The effect of gamma-radiation on the level of nuclear DNA damage in onion seedlings (Allium-test) was studied using the comet assay. DNA breaks were first found in cells of onion seedlings exposed to low-dose radiation (
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