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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N.N., Gladyshev M.I., Makhutova O.N., Kalachova G.S., Kravchuk E.S., Ivanova E.A.
Заглавие : Associating particulate essential fatty acids of the omega 3 family with phytoplankton species composition in a Siberian reservoir
Колич.характеристики :14 с
Место публикации : Freshw. Biol.: BLACKWELL PUBLISHING LTD, 2004. - Vol. 49, Is. 9. - P1206-1219. - ISSN 0046-5070, DOI 10.1111/j.1365-2427.2004.01263.x
Примечания : Cited References: 48
Предметные рубрики: ENCLOSED EXPERIMENTAL ECOSYSTEM
FOOD QUALITY
BENTHIC INVERTEBRATES
GREEN-ALGAE
CYANOBACTERIA
DAPHNIA
MICROALGAE
ZOOPLANKTON
LAKE
APHANIZOMENON
Ключевые слова (''Своб.индексиров.''): cyanobacteria--diatoms--fatty acids--food quality--species composition
Аннотация: 1. We studied variation in the composition of fatty acids in the seston of a small freshwater reservoir with changes in phytoplankton composition during four growth seasons. We focused on the dynamics of the omega3 fatty acids because of their potential importance for zooplankton nutrition. 2. Total diatoms were related to the 20:5omega3 fatty acid (eicosapentaenoic, EPA) content in seston. Among two dominant diatom genera, Cyclotella was not associated with EPA content. In contrast, there was a significant correlation between Stephanodiscus and the percentage contribution and content of EPA throughout the study. Hence, freshwater diatoms can differ strongly in content of the essential EPA. 3. We considered abundant cyanobacteria as a potential source of 18:3omega3 fatty acid (linolenic, ALA) to aquatic food webs. Among four dominant cyanobacteria species, two (Anabaena flos-aquae and Planktothrix agardhii) showed significant correlation with the ALA content of the seston, while the other two (Aphanizomenon flos-aquae and Microcystis aeruginosa) did not. 4. Dinophyta had a relatively high level of 22:6omega3 (docosahexaenoic, DHA) for freshwater species and can be also a source of EPA to aquatic food webs. 5. Our results show that various species of diatoms as well as cyanobacteria can be of contrasting nutritional value for zooplankton because of their different content of the essential PUFAs. Diatoms, which are low in EPA, could not be considered as a valuable food, while some field populations of cyanobacteria might be valuable sources of essential ALA.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gaevsky N.A., Kolmakov V.I., Anishchenko O.V., Gorbaneva T.B.
Заглавие : Using DCMU-fluorescence method for the identification of dominant phytoplankton groups
Колич.характеристики :12 с
Место публикации : J. Appl. Phycol.: SPRINGER, 2005. - Vol. 17, Is. 6. - P483-494. - ISSN 0921-8971, DOI 10.1007/s10811-005-2903-x
Примечания : Cited References: 28
Предметные рубрики: IN-VIVO
CHLOROPHYLL FLUORESCENCE
FLUOROMETRIC METHOD
SITU
PHOTOSYNTHESIS
CYANOBACTERIA
ALGAE
EVOLUTION
CULTURES
LAKES
Ключевые слова (''Своб.индексиров.''): algae--chlorophyll--fluorescence--vizualization
Аннотация: For the identification of ecologically significant dominant groups of phytoplanktonic algae a polychromatic DCMU-induced fluorescence method is recommended. A special fluorometer equipped with a system of replaceable filters is used to differentiate three regions of the spectrum (410 +/- 20, 510 +/- 20 and 540 +/- 10 nm) that can excite the basic light-harvesting pigments. Total and differential (for every algal taxon studied) chlorophyll a calculated from the fluorescence signals is in good agreement with biomass estimates from direct cell counts for several different trophic types of aquatic systems. This is made possible by the vizualization of the ratios of fluorescence signal values in their own coordinates: first, to decide whether it is necessary to correct linear equations in order to eliminate negative solutions; second, to determine the possibility of nulling the negative solution if a point is situated close to a side of the triangle; and third, to reduce the number of linear algebraic equations to two if the points are situated along one of the triangle sides or to one if the points are gathered at the apex. The polychromatic DCMU-induced fluorescence method can be used for monitoring natural phytoplankton populations to detect changes in their taxonomic structure.
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