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полный информационныйкраткий
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1.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Setkov N.A.
Заглавие : Protein synthesis inhibitors stimulated DNA synthesis in resting cells NIT 3T3
Колич.характеристики :4 с
Место публикации : Dokl. Akad. Nauk: MEZHDUNARODNAYA KNIGA, 1996. - Vol. 347, Is. 4. - P552-555. - ISSN 0869-5652
Примечания : Cited References: 15
Предметные рубрики: GROWTH
FIBROBLASTS
NUCLEI
IDENTIFICATION
CYCLOHEXIMIDE
FUSION
PERIOD
STATE
WOS
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2.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : SETKOV N.A., KAZAKOV V.N., ROSENWALD I.B., MAKAROVA G.F., EPIFANOVA O.I.
Заглавие : PROTEIN-SYNTHESIS INHIBITORS, LIKE GROWTH-FACTORS, MAY RENDER RESTING 3T3 CELLS COMPETENT FOR DNA-SYNTHESIS - A AUTORADIOGRAPHIC AND CELL-FUSION STUDY
Колич.характеристики :11 с
Место публикации : Cell Prolif.: BLACKWELL SCIENCE LTD, 1992. - Vol. 25, Is. 3. - P181-191. - ISSN 0960-7722, DOI 10.1111/j.1365-2184.1992.tb01393.x
Примечания : Cited References: 20
Предметные рубрики: C-MYC
CYCLOHEXIMIDE
FIBROBLASTS
EXPRESSION
INDUCTION
GENES
FOS
Аннотация: Serum-deprived (0.1-0.2%) resting NIH 3T3 mouse fibroblasts pre-incubated with cycloheximide (7.5-mu-g/ml), or puromycin (10-mu-g/ml), were fused with stimulated cells taken 10 h after changing the medium to one containing 10% serum, and DNA synthesis was investigated in the nuclei of monokaryons, homodikaryons and heterodikaryons using radioautography with the double-labelling technique. Pre-incubation of resting cells with inhibitors of protein synthesis for 1-4 h abolished their ability to suppress DNA synthesis in stimulated nuclei in heterokaryons. Three hours after the removal of cycloheximide from the medium, the resting cells acquired once again the inhibitory capacity for entry of stimulated nuclei into the S period. This inhibitory influence disappeared also in the case of post-fusion cycloheximide application as well as following an 8-12 h pre-treatment of resting cells with actinomycin D (1-mu-g/ml) prior to fusion. Pre-incubation of resting cells for 12 h with PDGF (1 u/ml-1) followed by an 8-48 h incubation in serum-free medium stimulated the onset of DNA synthesis. A brief exposure (45 min) of resting cells to cycloheximide (7.5-mu-g/ml), or puromycin (7.5-mu-g/ml), exerted a similar effect, inducing by itself the entry of cells into the S period. The results support the assumption that acquirement, by resting cells, of competence for DNA replication includes as a necessary step the down-regulation of intracellular growth inhibitors whose formation depends on protein synthesis.
WOS
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