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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : POPOVA T.G., SADOVSKII M.G.
Заглавие : INTRONS DIFFER FROM EXONS BY THEIR REDUNDANCY
Колич.характеристики :5 с
Место публикации : Genetika: MEZHDUNARODNAYA KNIGA, 1995. - Vol. 31, Is. 10. - P1365-1369. - ISSN 0016-6758
Примечания : Cited References: 12
Предметные рубрики: SEQUENCES
DNA
Аннотация: This paper is devoted to an analysis of the intrinsic structure of the gene from the point of view of the redundancy of different structural units - exons and introns. Human genes for which the exon-intron structure has - been clearly established were Studied. The redundancy of each exon and intron in the gene was determined. It was shown that, in human genes, introns are more redundant than exons. Redundancy is determined as the smallest length of a word (oligonucleotide) beginning with which all words in the studied nucleotide sequence are found exactly once. Mechanisms leading to the disruption of the general pattern of ratios of redundancy of exons and introns are studied.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ranjan, Rajeev, Esimbekova, Elena N., Kratasyuk, Valentina A.
Заглавие : Rapid biosensing tools for cancer biomarkers
Колич.характеристики :13 с
Коллективы : Russian Foundation for Basic Research [16-34-60100, 16-06-00439]; state budget allocated to the fundamental research Russian Academy of Sciences Project [01201351504]
Место публикации : Biosens. Bioelectron.: ELSEVIER ADVANCED TECHNOLOGY, 2017. - Vol. 87. - С. 918-930. - ISSN 0956-5663, DOI 10.1016/j.bios.2016.09.061. - ISSN 1873-4235(eISSN)
Примечания : Cited References:115. - The research was partially supported by the Russian Foundation for Basic Research (Project no. 16-34-60100 and No. 16-06-00439), the state budget allocated to the fundamental research Russian Academy of Sciences (Project no. 01201351504).
Предметные рубрики: POINT-OF-CARE
HIGHLY SENSITIVE DETECTION
ACOUSTIC-WAVE BIOSENSOR
DNA
Ключевые слова (''Своб.индексиров.''): biosensor--cancer biomarker--functional nanomaterials--point-of-care--devices--microfluidics
Аннотация: The present review critically discusses the latest developments in the field of smart diagnostic systems for cancer biomarkers. A wide coverage of recent biosensing approaches involving aptamers, enzymes, DNA probes, fluorescent probes, interacting proteins and antibodies in vicinity to transducers such as electrochemical, optical and piezoelectric is presented. Recent advanced developments in biosensing approaches for cancer biomarker owes much credit to functionalized nanomaterials due to their unique opto-electronic properties and enhanced surface to volume ratio. Biosensing methods for a plenty of cancer biomarkers has been summarized emphasizing the key principles involved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Davydova, Anna, Vorobyeva, Mariya, Bashmakova, Eugenia, Vorobjev, Pavel, Krasheninina, Olga, Tupikin, Alexey, Kabilov, Marsel, Krasitskaya, Vasilisa, Frank, Ludmila, Venyaminova, Alya
Заглавие : Development and characterization of novel 2 '-F-RNA aptamers specific to human total and glycated hemoglobins
Колич.характеристики :8 с
Коллективы : Russian Science Foundation [16-14-10296]
Место публикации : Anal. Biochem.: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2019. - Vol. 570. - С. 43-50. - ISSN 0003-2697, DOI 10.1016/j.ab.2019.02.004. - ISSN 1096-0309(eISSN)
Примечания : Cited References:32. - We want to thank Dr. Alexander Lomzov (ICBFM SB RAS) for his valuable assistance with circular dichroism studies. The work was supported by the Russian Science Foundation (grant number 16-14-10296).
Предметные рубрики: RNA APTAMER
SEQUENCES
PROTEIN
DNA
Аннотация: Aptamers are short DNA and RNA fragments which bind their molecular targets with affinity and specificity comparable to those of antibodies. Here, we describe the selection of novel 2'-F-RNA aptamers against total human hemoglobin or its glycated form HbA1c. After SELEX and high-throughput sequencing of the enriched libraries, affinities and specificities of candidate aptamers and their truncated variants were examined by the solid-phase bioluminescent assay. As a result, we identified aptamers specific to both hemoglobins or only glycated HbA1c. The developed 2'-F-RNA aptamers have shown their applicability for detection of total and glycated hemoglobin in one sample.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shen, Y.a., Chen, Sheng-Chung, Lai, Mei-Chin, Huang, Hsing-Hua, Chiu, Hsiu-Hui, Tang, Sen-Lin, Rogozin, Denis Yu, Degermendzhy, Andrey G.
Заглавие : Methanolobus halotolerans sp. nov., isolated from the saline Lake Tus in Siberia
Колич.характеристики :8 с
Коллективы : Ministry of Education, Taiwan, ROC under the ATU planMinistry of Education, Taiwan; thematic project funding of Taiwan-Russian Joint Project [NSC99-2923-B-001-001-MY3, NSC102-2923-B-001-004]; Ministry of Science and Technology, TaiwanMinistry of Science and Technology, Taiwan [MOST103/104/105/106/107-3113-M-005-001]; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [14-04-01060-a]
Место публикации : Int. J. Syst. Evol. Microbiol.: MICROBIOLOGY SOC, 2020. - Vol. 70, Is. 10. - С. 5586-5593. - ISSN 1466-5026, DOI 10.1099/ijsem.0.004453. - ISSN 1466-5034(eISSN)
Примечания : Cited References:38. - This work was supported by grants from the Ministry of Education, Taiwan, ROC under the ATU plan (to M.C.L.), by the thematic project funding of Taiwan-Russian Joint Project (NSC99-2923-B-001-001-MY3 and NSC102-2923-B-001-004 to S.L.T.), by the Ministry of Science and Technology (MOST103/104/105/106/107-3113-M-005-001 to M.C.L.), Taiwan and the Russian Foundation for Basic Research, Grant No. 14-04-01060-a (to D.Y.R. and A.G.D.).
Предметные рубрики: TAIWANENSIS SP NOV.
METHYLOTROPHIC METHANOGEN
SEDIMENTS
ACCURATE
DNA
Аннотация: A halotolerant, psychrotolerant and methylotrophic methanogen, strain SY-01(T), was isolated from the saline Lake Tus in Siberia. Cells of strain SY-01(T) were non-motile, cocci and 0.8-1.0 mu m in diameter. The only methanogenic substrate utilized by strain SY-01(T) was methanol. The temperature range of growth for strain SY-01(T) was from 4 to 40 degrees C and the optimal temperature for growth was 30 degrees C. The pH range of growth was from pH 7.2 to 9.0, with optimal growth at pH 8.0. The NaCl range of growth was 0-1.55 M with optimal growth at 0.51 M NaCl. The G+C content of the genome of strain SY-01(T) was 43.6 mol% as determined by genome sequencing. Phylogenetic analysis revealed that strain SY-01(T) was most closely related to Methanolobus zinderi SD1(T) (97.3% 16S rRNA gene sequence similarity), and had 95.5-97.2 % similarities to other Methanolobus species with valid names. Genome relatedness between strain SY-01(T) and DSM 21339(T) was computed using average nucleotide identity and digital DNA-DNA-hybridization, which yielded values of 79.7 and 21.7 %, respectively. Based on morphological, phenotypic, phylogenetic and genomic relatedness data presented here, it is evident that strain SY-01(T) represents a novel species of the genus Methanolobus, and the name Methanolobus halotolerans sp. nov. is proposed. The type strain is SY-01(T) (=BCRC AR10051(T)=NBRC 113166(T)=DSM 107642(T)).
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