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1.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Puzyr A.P.
Заглавие : Effect of the intracellular poly-beta-hydroxybutyrate pool on the growth of a hydrogen-oxidizing bacterium under nonoptimum conditions
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1996. - Vol. 65, Is. 5. - С. 521-524. - 4. - ISSN 0026-2617
Примечания : Cited References: 16
Предметные рубрики: MEMBRANES
Аннотация: Growth of the hydrogen-oxidizing bacterium Alcaligenes eutrophus under nonoptimum conditions (acidic and alkaline pH, elevated and lowered temperatures, absence of exogenous sources of carbon and energy) was studied in relation to the initial intracellular concentration of poly-beta-hydroxybutyrate (PHB), which varied from virtually zero to 60-70% of the dry biomass. An initial PHB pool of greater than 30-40% provided for a 2- to 2.5-fold higher biomass yield in acidic and alkaline media or at nonoptimum temperatures as compared with cultures initially containing no polymer. The intracellular PHB pool also provides for the growth of the culture in the absence of exogenous carbon sources and its survival (without growth) with no exogenous energy supply.
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2.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T..., Goncharov D..., Sukovatyi A..., Shabanov A..., Nikolaeva E..., Shishatskaya E...
Заглавие : Electrospinning of polyhydroxyalkanoate fibrous scaffolds: effects on electrospinning parameters on structure and properties
Колич.характеристики :24 с
Коллективы : Project 'Biotechnologies of novel biomaterials: innovative biopolymers and devices for biomedicine' [1, 11.G34.31.0013]; Government of the Russian Federation [220]; RF President for supporting young Doctors of Sciences [MD-3112.2012.4]
Место публикации : J. Biomater. Sci.-Polym. Ed.: TAYLOR & FRANCIS LTD, 2014. - Vol. 25, Is. 4. - С. 370-393. - ISSN 0920-5063, DOI 10.1080/09205063.2013.862400
Примечания : Cited References: 52. - This study was financially supported by Project 'Biotechnologies of novel biomaterials: innovative biopolymers and devices for biomedicine' (Agreement No. 1 of 15.02.2013 to Agreement No. 11.G34.31.0013) in accordance with Resolution No. 220 of the Government of the Russian Federation of April 9, 2010, 'On measures designed to attract leading scientists to the Russian institutions of higher learning' and Grant of the RF President for supporting young Doctors of Sciences No. MD-3112.2012.4.
Предметные рубрики: TISSUE ENGINEERING APPLICATIONS
FIBER MATS
POLY 3-HYDROXYBUTYRATE
POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)
BIOCOMPATIBILITY
PROLIFERATION
FABRICATION
NANOFIBERS
COPOLYMERS
MEMBRANES
Ключевые слова (''Своб.индексиров.''): electrospinning--polyhydroxyalkanoates--ultrafine fibers--physical-mechanical properties--fibroblast cells
Аннотация: In this study, electrospinning was used to prepare ultrafine fibers from PHAs with different chemical compositions: P(3HB) and copolymers: P(3HB-co-4HB), P(3HB-co-3HV), and P(3HB-co-3HHx). The main process parameters that influence ultrafine fiber diameter and properties (polymer concentration, solution feeding rate, working distance, and applied voltage) have been investigated and their effects evaluated. The study revealed electrospinning parameters for the production of high-quality ultrafine fibers and determined which parameters should be varied to tailor the properties of the products. This study is the first to compare biological and physical-mechanical parameters of PHAs with different chemical compositions as dependent upon the fractions of monomers constituting the polymers and ultrafine fiber orientation. Mechanical strength of aligned ultrafine fibers prepared from different PHAs is higher than that of randomly oriented ones; no significant effect of ultrafine fiber orientation on surface properties has been found. None of the fibrous scaffolds produced by electrospinning from PHAs had any adverse effects on attachment, growth, and viability of NIH 3T3 mouse fibroblast cells, and all of them were found to be suitable for tissue engineering applications.
WOS,
Scopus
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