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Общее количество найденных документов : 8
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondar V.S., Boersma M.G., Golovlev E.L., Vervoort J..., Van Berkel WJH, Finkelstein Z.I., Solyanikova I.P., Golovleva L.A., Rietjens IMCM
Заглавие : (19)F NMR study on the biodegradation of fluorophenols by various Rhodococcus species
Колич.характеристики :12 с
Место публикации : Biodegradation: SPRINGER, 1998. - Vol. 9: NMR in Environmental Sciences Symposium (OCT 24, 1997, EDE, NETHERLANDS), Is. 6. - С. 475-486. - ISSN 0923-9820, DOI 10.1023/A:1008391906885
Примечания : Cited References: 37
Предметные рубрики: NUCLEAR-MAGNETIC-RESONANCE
PSEUDOMONAS-PUTIDA MT-2
PHENOL HYDROXYLASE
BACTERIAL-DEGRADATION
3-OXOADIPATE PATHWAY
FLUOROBENZOIC ACID
CONVERSION
METABOLISM
CYCLOISOMERASES
2-CHLORO-CIS,CIS-MUCONATE
Ключевые слова (''Своб.индексиров.''): biodegradation--fluorophenols--(19)f nmr--oxidative defluorination--rhodococcus species
Аннотация: Of all NMR observable isotopes (19)F is the one perhaps most convenient for studies on biodegradation of environmental pollutants. The reasons underlying this potential of (19)F NMR are discussed and illustrated on the basis of a study on the biodegradation of fluorophenols by four Rhodococcus strains. The results indicate marked differences between the biodegradation pathways of fluorophenols among the various Rhodococcus species. This holds not only for the level and nature of the fluorinated biodegradation pathway intermediates that accumulate, but also for the regioselectivity of the initial hydroxylation step. Several of the Rhodococcus species contain a phenol hydroxylase that catalyses the oxidative defluorination of ortho-fluorinated di- and trifluorophenols. Furthermore, it is illustrated how the (19)F NMR technique can be used as a tool in the process of identification of an accumulated unknown metabolite, in this case most likely 5-fluoromaleylacetate. Altogether, the (19)F NMR technique proved valid to obtain detailed information on the microbial biodegradation pathways of fluorinated organics, but also to provide information on the specificity of enzymes generally considered unstable and, for this reason, not much studied so far.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N.N., Kalacheva G.S., Gladyshev M.I.
Заглавие : Effect of temperature on the extracellur free fatty acid composition in cultures of green and blue-green algae
Колич.характеристики :4 с
Место публикации : Dokl. Akad. Nauk: MEZHDUNARODNAYA KNIGA, 1999. - Vol. 367, Is. 4. - С. 567-570. - ISSN 0869-5652
Примечания : Cited References: 9
Предметные рубрики: METABOLISM
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Trusova M.Y., Tyul'kova N.A., Puzyr' A.P.
Заглавие : Intracellular degradation of poly-beta-hydroxybutyrate in the hydrogen-oxidizing bacterium Alcaligenes eutrophus
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1999. - Vol. 68, Is. 1. - С. 52-57. - 6. - ISSN 0026-2617
Примечания : Cited References: 16
Предметные рубрики: METABOLISM
Ключевые слова (''Своб.индексиров.''): hydrogen-oxidizing bacteria--poly-beta-hydroxybutyrate--degradation--cell size
Аннотация: The intracellular degradation of poly-beta-hydroxybutyrate (PHB) in Alcaligenes eutrophus culture was found to occur at different: rates, depending on growth conditions, The degradation of PHB probably allows protein. synthesis and cell growth under nonoptimal environmental conditions. The isolated native granules of poly-beta-hydroxybutyrate were stable, unless they were isolated from biomass subjected to freezing-thawing treatment. Degradation of isolated granules could also be stimulated by increasing the ionic strength of the medium or-adding cell-free extracts, It is anticipated that bacterial cells contain soluble factors that either stimulate or inhibit the degradation of PHB granules.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondar V.S., Boersma M.G., van Berkel WJH, Finkelstein Z.I., Golovlev E.L., Baskunov B.P., Vervoort J..., Golovleva L.A., Rietjens IMCM
Заглавие : Preferential oxidative dehalogenation upon conversion of 2-halophenols by Rhodococcus opacus 1G
Колич.характеристики :10 с
Место публикации : FEMS Microbiol. Lett.: ELSEVIER SCIENCE BV, 1999. - Vol. 181, Is. 1. - С. 73-82. - ISSN 0378-1097, DOI 10.1111/j.1574-6968.1999.tb08828.x
Примечания : Cited References: 30
Предметные рубрики: PHENOL HYDROXYLASE
POLYCHLORINATED PHENOLS
MICROBIAL-DEGRADATION
TRICHOSPORON-CUTANEUM
METABOLISM
PURIFICATION
REGIOSELECTIVITY
CHLOROAROMATICS
COMPONENT
BENZOATE
Ключевые слова (''Своб.индексиров.''): phenol hydroxylase--oxidative dehalogenation--2-halophenol--f-19 nmr--rhodococcus opacus
Аннотация: The regiospecificity of hydroxylation of C2-halogenated phenols by Rhodococcus opacus 1G was investigated. Oxidative defluorination at the C2 position ortho with respect to the hydroxyl moiety was preferred over hydroxylation at the non fluorinated C6 position for all 2-fluorophenol compounds studied. Initial hydroxylation of 2,3,5-trichlorophenol resulted in the exclusive formation of 3,5-dichlorocatechol. These results indicate that, in contrast to all other phenol ortho-hydroxylases studied so far, phenol hydroxylase from R. opacus 1G is capable of catalyzing preferential oxidative defluorination but also oxidative dechlorination. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kalacheva G.S., Zhila N.O., Volova T.G.
Заглавие : Lipids of the green alga Botryococcus cultured in a batch mode
Колич.характеристики :7 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 2001. - Vol. 70, Is. 3. - P256-262. - ISSN 0026-2617, DOI 10.1023/A:1010438908047
Примечания : Cited References: 17
Предметные рубрики: BRAUNII
HYDROCARBON
METABOLISM
RACE
Ключевые слова (''Своб.индексиров.''): botryococcus braunii--batch culture--lipids--fatty acids
Аннотация: The lipid fraction of the green alga Botryococcus cultured in a batch mode was found to contain polar lipids (more than 50% of the total lipids), di- and triacylglycerols, sterols and their eaters, free fatty acids, and hydrocarbons. In aging culture, the content of polar lipids somewhat decreased and that of triacylglycerols increased by more than four times. The content of hydrocarbons in the algal biomass did not exceed 0.9% and depended little on the culture age. Intracellular lipids contained saturated and unsaturated (mono-, di-, and trienoic) fatty acids. The maximum content of C-16:3 and alpha -C-18 :3 fatty acids (up to 35% of the total fatty acids) was detected in the phase of active growth. The extracellular and intracellular lipids of the al,oa differed in the proportion of particular lipids and in the fatty acid pattern.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Altukhova O.V.
Заглавие : Autotrophic synthesis of polyhydroxyalkanoates by the bacteria Ralstonia eutropha in the presence of carbon monoxide
Колич.характеристики :4 с
Место публикации : Appl. Microbiol. Biotechnol.: SPRINGER-VERLAG, 2002. - Vol. 58, Is. 5. - P675-678. - ISSN 0175-7598, DOI 10.1007/s00253-002-0941-8
Примечания : Cited References: 35
Предметные рубрики: PSEUDOMONAS-OLEOVORANS
ALCALIGENES-EUTROPHUS
COAL
CULTIVATION
METABOLISM
SUBSTRATE
CULTURE
CO2
H-2
O-2
Аннотация: It has been found that the carbon monoxide (CO)-resistant strain of the hydrogen bacteria Ralstonia eutropha B5786 is able to synthesise polyhydroxyalkanoates (PHAs) in the presence of CO under autotrophic conditions. This strain, grown on model gas mixtures containing 5-25% CO v/v), accumulates up to 70-75% (of absolutely dry matter) PHA, without significant variation in the yield coefficient on hydrogen. No suppression of the activities of the key enzymes of PHA synthesis (beta-ketothiolase, acetoacetyl-CoA-reductase, butyrate dehydrogenase and poly-3-hydroxybutyrate synthase) was recorded. The PHA synthesised is a copolymer containing mostly beta-hydroxybutyrate (more than 99 mol%) with trace amounts of beta-hydroxyvalerate. The investigated properties of the polymer (molecular weight, crystallinity, temperature characteristics) do not differ from those of the polymer synthesised on electrolytic hydrogen.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kalacheva G.S., Zhila N.O., Volova T.G., Gladyshev M.I.
Заглавие : The effect of temperature on the lipid composition of the green alga Botryococcus
Колич.характеристики :8 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 2002. - Vol. 71, Is. 3. - P286-293. - ISSN 0026-2617, DOI 10.1023/A:1015898426573
Примечания : Cited References: 14
Предметные рубрики: METABOLISM
CULTURES
BRAUNII
Ключевые слова (''Своб.индексиров.''): green alga--lipids--fatty acids--temperature
Аннотация: The lipid composition of the green alga Botryococcus was studied at three different cultivation temperatures: suboptimal (18degreesC), optimal (25degreesC), and supraoptimal (32degreesC). Cultivation at the supraoptimal temperature was found to considerably inhibit the synthesis of nearly all intracellular lipids, except for triacylglycerides, and to influence their fatty acid composition. In particular, the content of trienoic fatty acids was significantly lower at the supraoptimal than at the optimal cultivation temperature. At the same time, the fatty acid composition of the extracellular lipids of the alga virtually did not depend on cultivation temperature.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mitiouchkina, Tatiana, Mishin, Alexander S., Somermeyer, Louisa Gonzalez, Markina, Nadezhda M., Chepurnyh, Tatiana, V, Guglya, Elena B., Karataeva, Tatiana A., Palkina, Kseniia A., Shakhova, Ekaterina S., Fakhranurova, Liliia, I, Chekova, Sofia, V, Tsarkova, Aleksandra S., Golubev, Yaroslav, V, Negrebetsky, Vadim V., Dolgushin, Sergey A., Shalaev, Pavel, V, Shlykov, Dmitry, Melnik, Olesya A., Shipunova, Victoria O., Deyev, Sergey M., Bubyrev, Andrey, I, Pushin, Alexander S., Choob, Vladimir V., Dolgov, Sergey, V, Kondrashov, Fyodor A., Yampolsky, Ilia, V, Sarkisyan, Karen S.
Заглавие : Plants with genetically encoded autoluminescence
Колич.характеристики :7 с
Коллективы : Planta LLC; Skolkovo Innovation Centre; MRC London Institute of Medical Sciences [UKRI MC-A658-5QEA0]; Imperial College Research Fellowship; Institute of Bioorganic Chemistry of the Russian Academy of Sciences.ore Facility (CKP IBCH - Russian Ministry of Education and Science Grant) [RFMEFI62117X0018]; ERC grant [771209-CharFL]; European Union's Horizon 2020 Research and Innovation Programme under Marie Sklodowska-Curie Grant [665385]; Russian Science Foundation grantRussian Science Foundation (RSF) [19-74-10102, 17-14-01169p, 16-14-00052p]; Ministry of Science and Higher Education of the Russian Federation [075-15-2019-1789]; [075-15-2019-411]
Место публикации : Nat. Biotechnol.: NATURE PUBLISHING GROUP, 2020. - Article in press. - ISSN 1087-0156, DOI 10.1038/s41587-020-0500-9. - ISSN 1546-1696(eISSN)
Примечания : Cited References:17. - This study was designed, performed and funded by Planta LLC. We thank K. Wood for assisting in manuscript development. Planta acknowledges support from the Skolkovo Innovation Centre. We thank D. Bolotin and the Milaboratory (milaboratory. com) for access to computing and storage infrastructure. We thank S. Shakhov for providing photography equipment. The Synthetic Biology Group is funded by the MRC London Institute of Medical Sciences (UKRI MC-A658-5QEA0, K.S.S.). K.S.S. is supported by an Imperial College Research Fellowship. Experiments were partially carried out using equipment provided by the Institute of Bioorganic Chemistry of the Russian Academy of Sciences.ore Facility (CKP IBCH; supported by the Russian Ministry of Education and Science Grant RFMEFI62117X0018). The F.A.K. lab is supported by ERC grant agreement 771209-CharFL. This project received funding from the European Union's Horizon 2020 Research and Innovation Programme under Marie Sklodowska-Curie Grant Agreement 665385. K.S.S. acknowledges support by President's Grant 075-15-2019-411. Design and assembly of some of the plasmids was supported by Russian Science Foundation grant 19-74-10102. Imaging experiments were partially supported by Russian Science Foundation grant 17-14-01169p. LC-MS/MS analyses of extracts were supported by Russian Science Foundation grant 16-14-00052p. Design and assembly of plasmids was partially supported by grant 075-15-2019-1789 from the Ministry of Science and Higher Education of the Russian Federation allocated to the Center for Precision Genome Editing and Genetic Technologies for Biomedicine.
Предметные рубрики: METABOLISM
LIBRARY
Аннотация: Autoluminescent plants engineered to express a bacterial bioluminescence gene cluster in plastids have not been widely adopted because of low light output. We engineered tobacco plants with a fungal bioluminescence system that converts caffeic acid (present in all plants) into luciferin and report self-sustained luminescence that is visible to the naked eye. Our findings could underpin development of a suite of imaging tools for plants.
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