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полный	информационный	краткий

Поисковый запрос: (<.>S=METAGENOME<.>)

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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Chen, Yu-Hsiang, Chiang, Pei-Wen, Rogozin, Denis Yu, Degermendzhy, Andrey G., Chiu, Hsiu-Hui, Tang, Sen-Lin
Заглавие : Salvaging high-quality genomes of microbial species from a meromictic lake using a hybrid sequencing approach
Колич.характеристики :12 с
Коллективы : Ministry of Science and Technology in Taiwan through the Taiwan-Russia Joint Project [NSC 102-2923-B-001-004-MY3]; Russian Foundation for Basic Research GrantRussian Foundation for Basic Research (RFBR) [21-54-52001, MOST 105-2923-B-001-001-MY3]; Taiwan International Graduate Program (TIGP)
Место публикации : Commun. Biol.: NATURE PORTFOLIO, 2021. - Vol. 4, Is. 1. - Ст.996. - ISSN 2399-3642(eISSN), DOI 10.1038/s42003-021-02510-6
Примечания : Cited References:99. - Y This work was supported by the Ministry of Science and Technology in Taiwan through the Taiwan-Russia Joint Project Grant NSC 102-2923-B-001-004-MY3 and Russian Foundation for Basic Research Grant 21-54-52001 and MOST 105-2923-B-001-001-MY3. Y.H.C. would like to acknowledge the Taiwan International Graduate Program (TIGP) for its fellowship towards his graduate studies. We would like to thank Noah Last of Third Draft Editing for his English language editing.
Предметные рубрики: DIMETHYL SULFIDE
SULFUR BACTERIUM
SP-NOV.
CHLAMYDIALES
METAGENOME
Аннотация: Most of Earth's bacteria have yet to be cultivated. The metabolic and functional potentials of these uncultivated microorganisms thus remain mysterious, and the metagenome-assembled genome (MAG) approach is the most robust method for uncovering these potentials. However, MAGs discovered by conventional metagenomic assembly and binning are usually highly fragmented genomes with heterogeneous sequence contamination. In this study, we combined Illumina and Nanopore data to develop a new workflow to reconstruct 233 MAGs-six novel bacterial orders, 20 families, 66 genera, and 154 species-from Lake Shunet, a secluded meromictic lake in Siberia. With our workflow, the average N50 of reconstructed MAGs greatly increased 10-40-fold compared to when the conventional Illumina assembly and binning method were used. More importantly, six complete MAGs were recovered from our datasets. The recovery of 154 novel species MAGs from a rarely explored lake greatly expands the current bacterial genome encyclopedia. Chen and colleagues develop a workflow for assembling high quality metagenome-associated genomes for microbial species using long and short reads, in this case from a meromictic lake. A full, detailed workflow is provided in for use by the community.
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