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Ultrafast fluorescence relaxation spectroscopy of 6,7-dimethyl-(8-ribityl)-lumazine and riboflavin, free and bound to antenna proteins from bioluminescent bacteria [Text] / V. N. Petushkov [et al.]> // J. Phys. Chem. B. - 2003. - Vol. 107, Is. 39. - P. 10934-10939, DOI 10.1021/jp034266e. - Cited References: 52
. - ISSN 1520-6106РУБ Chemistry, Physical Рубрики: TIME-RESOLVED FLUORESCENCE VIBRIO-FISCHERI Y1 FEMTOSECOND SOLVATION DYNAMICS FLAVIN ADENINE-DINUCLEOTIDE PHOTOBACTERIUM-LEIOGNATHI BIOLOGICAL WATER SOLVENT DYNAMICS DIELECTRIC-RELAXATION MOLECULAR-DYNAMICS TRYPTOPHAN Аннотация: The solvation dynamics of interesting bioluminescent chromophores have been determined, using subpicosecond and wavelength-resolved fluorescence spectroscopy, in combination with global analysis of the multidimensional data sets. The systems investigated comprise the free ligands 6,7-dimethyl-(8-ribityl)-lumazine (lumazine) and riboflavin in an aqueous buffer and both ligands when noncovalently bound to two bacterial bioluminescent antenna proteins: lumazine protein (from Photobacterium leiognathi) and the blue fluorescent protein (from Vibrio fischeri Y1). Fluorescence spectral relaxation of the free ligands is complete within a few picoseconds. Subsequently, the fluorescence intensity increases by similar to7% on a time scale of 15-30 ps. Fluorescence spectral relaxation of the protein-bound ligands is largely complete within 1 ps but reveals a small red shift with a minor, but distinctly longer, relaxation time than that of the free ligands, which is tentatively assigned to the relaxation of protein-bound water in the vicinity of the excited chromophore.
WOS Держатели документа: Univ Wageningen & Res Ctr, Biochem & Biophys Lab, MicroSpect Ctr, NL-6703 HA Wageningen, Netherlands Vrije Univ Amsterdam, Fac Sci & Engn, Dept Phys & Astron, NL-1081 HV Amsterdam, Netherlands Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA Vrije Univ Amsterdam, Fac Earth & Life Sci, Dept Biol Struct, NL-1081 HV Amsterdam, Netherlands Russian Acad Sci, Inst Biophys, Krasnoyarsk 660036, Russia ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50 Доп.точки доступа: Petushkov, V.N.; van Stokkum, IHM; Gobets, B...; van Mourik, F...; Lee, J...; van Grondelle, R...; Visser, AJWG Найти похожие
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