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1.

Вид документа : Статья из сборника (выпуск монографической серии)
Шифр издания :
Автор(ы) : Frank, Ludmila A., Krasitskaya, Vasilisa V.
Заглавие : Application of Enzyme Bioluminescence for Medical Diagnostics
Колич.характеристики :23 с
Место публикации : Adv. Biochem. Eng. Biotechnol.: SPRINGER-VERLAG BERLIN, 2014. - Vol. 144. - С. 175-197. - (Advances in Biochemical Engineering-Biotechnology). - , DOI 10.1007/978-3-662-43385-0_6
Примечания : Cited References:63
Предметные рубрики: RESONANCE ENERGY-TRANSFER
POLYMERASE-CHAIN-REACTION
LUCIFERASE
Ключевые слова (''Своб.индексиров.''): bioluminescence--ca2+-regulated photoprotein--diagnostics--immunoassay--luciferase--nucleic acid hybridization assay
Аннотация: Nowadays luciferases are effectively used as analytical instruments in a great variety of research fields. Of special interest are the studies dealing with elaboration of novel analytical systems for the purposes of medical diagnostics. The ever-expanding spectrum of clinically important analytes accounts for the increasing demand for new techniques for their detection. In this chapter we have made an attempt to summarize the results on applications of luciferases as reporters in binding assays including immunoassay, nucleic acid hybridization assay, and so on. The data over the last 15 years have been analyzed and clearly show that luciferase-based assays, due to extremely high sensitivity, low cost, and the lack of need for skilled personnel, hold much promise for clinical diagnostics.
WOS
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2.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Frank L.A.
Заглавие : Ca2+-Regulated Photoproteins: Effective Immunoassay Reporters
Колич.характеристики :14 с
Коллективы :
Место публикации : Sensors: MDPI AG, 2010. - Vol. 10, Is. 12. - С. 11287-11300. - ISSN 1424-8220, DOI 10.3390/s101211287
Примечания : Cited References: 70. - This work was supported by the grant No. 76 of the Russian Academy of Sciences, Siberian Branch.
Предметные рубрики: POLYMERASE-CHAIN-REACTION
CYTOKINE MESSENGER-RNA
BIOLUMINESCENT IMMUNOASSAY
MYCOBACTERIUM-TUBERCULOSIS
BIOTINYLATED AEQUORIN
RECOMBINANT AEQUORIN
ANGSTROM RESOLUTION
FUSION PROTEIN
PCR ASSAY
OBELIN
Ключевые слова (''Своб.индексиров.''): bioluminescence--ca2+-regulated photoprotein--immunoassay--pcr-elisa--multiplex assay--re-engineered photoproteins
Аннотация: Ca2+-regulated photoproteins of luminous marine coelenterates are of interest and a challenge for researchers as a unique bioluminescent system and as a promising analytical instrument for both in vivo and in vitro applications. The proteins are comprehensively studied as to biochemical properties, tertiary structures, bioluminescence mechanism, etc. This knowledge, along with available recombinant proteins serves the basis for development of unique bioluminescent detection systems that are "self-contained", triggerable, fast, highly sensitive, and non-hazardous. In the paper, we focus on the use of photoproteins as reporters in binding assays based on immunological recognition element-bioluminescent immunoassay and hybridization immunoassay, their advantages and prospects.
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3.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vysotski E.S., Markova S.V., Frank L.A.
Заглавие : Calcium-regulated photoproteins of marine coelenterates
Колич.характеристики :13 с
Место публикации : Mol. Biol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2006. - Vol. 40, Is. 3. - С. 355-367. - ISSN 0026-8933, DOI 10.1134/S0026893306030022
Примечания : Cited References: 99
Предметные рубрики: BIOLUMINOMETRIC HYBRIDIZATION ASSAYS
HYDROID OBELIA-GENICULATA
GREEN-FLUORESCENT PROTEIN
POLYMERASE-CHAIN-REACTION
BIOLUMINESCENT IMMUNOASSAY
RECOMBINANT AEQUORIN
CRYSTAL-STRUCTURE
BIOTINYLATED AEQUORIN
ANGSTROM RESOLUTION
CA2+-REGULATED PHOTOPROTEINS
Ключевые слова (''Своб.индексиров.''): bioluminescence--obelin--aequorin--intracellular calcium--molecular diagnosis
Аннотация: Calcium-regulated photoproteins are bioluminescent proteins that are responsible for the luminescence of marine coelenterates. A photoprotein molecule is a stable enzyme-substrate complex consisting of a single polypeptide chain and an oxygen-preactivated substrate, 2-hydroperoxcoelenterazine, which is tightly but noncovalently bound with the protein. Bioluminescence is triggered by Ca2+ and results from decarboxylation of the substrate bound with the protein. This review considers the current information about the structure of photoproteins, the mechanism of the bioluminescent reaction, the function of particular amino acid residues of the active center in catalysis and the formation of the emitter, and the use of photoproteins in bioluminescent microanalysis.
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