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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Okhonin V..., Krylova S.M., Krylov S.N.
Заглавие : Nonequilibrium capillary electrophoresis of equilibrium mixtures, mathematical model
Колич.характеристики :6 с
Место публикации : Anal. Chem.: AMER CHEMICAL SOC, 2004. - Vol. 76, Is. 5. - P1507-1512. - ISSN 0003-2700, DOI 10.1021/ac035259p
Примечания : Cited References: 24
Предметные рубрики: SURFACE-PLASMON RESONANCE
PROTEIN-DNA INTERACTIONS
FLUORESCENCE POLARIZATION
COMPLEX-FORMATION
BINDING
RECOGNITION
DERIVATIVES
TOOL
Аннотация: We recently introduced a new electrophoretic method, nonequilibrium, capillary electrophoresis of equilibrium mixtures (NECEEM). NECEEM provides a unique way of finding kinetic and equilibrium parameters of the formation of intermolecular complexes from a single electropherogram and allows for the use of weak affinity probes in protein quantitation. In this work, we study theoretical bases of NECEEM by developing a mathematical model for the new method. By solving a system of partial differential equations with diffusion in linear approximation, we found the analytical solution for concentrations of components involved in complex formation as functions of time from the beginning of separation and position in the capillary. The nonnumerical nature of the solution makes it a powerful tool in studying the theoretical foundations of the NECEEM method and modeling experimental results. We demonstrate the use of the model for finding binding parameters of complex formation by nonlinear regression of NECEEM electropherograms obtained experimentally.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zotina, Tatiana, Dementyev, Dmitry, Alexandrova, Yuliyana
Заглавие : Long-term trends and speciation of artificial radionuclides in two submerged macrophytes of the Yenisei River: A comparative study of Potamogeton lucens and Fontinalis antipyretica
Колич.характеристики :15 с
Коллективы : Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR); Krasnoyarsk Regional Scientific Foundation [18-44-240003]
Место публикации : J. Environ. Radioact.: ELSEVIER SCI LTD, 2021. - Vol. 227. - Ст.106461. - ISSN 0265-931X, DOI 10.1016/j.jenvrad.2020.106461. - ISSN 1879-1700(eISSN)
Примечания : Cited References:37. - The authors are grateful to the staff of the Radioecology Laboratory and the head of the Laboratory, Dr. Alexander Bolsunovsky, to the staff of Analytical Laboratory (Institute of Biophysics SB RAS) for their help in sample collection and measurement and to Elena Krasova for linguistic check and improvements. We also appreciate comments of anonymous reviewers, which allowed us to improve our manuscript. The research was supported partly (in 2018-2020) by the grant of Russian Foundation for Basic Research and Krasnoyarsk Regional Scientific Foundation No.18-44-240003.
Предметные рубрики: HEAVY-METALS
BRYOPHYTES
CONTAMINATION
BIOMASS
PLANTS
CS-137
TOOL
Аннотация: Long-term trends of artificial radionuclides, from 2003 to 2018, in two abundant species of macrophytes, shining pondweed, Potamogeton lucens, and water moss, Fontinalis antipyretica, have been analyzed to estimate the indicative reliability of these two species as biomonitors of radioactive contamination in a river system and to quantify the decrease in the content of artificial radionuclides in the Yenisei River. Time-dependent trends of artificial radionuclides in the biomass of these species were similar, resulting in estimates of effective half-lives for Mn-54, Co-58, Co-60, Zn-65, Cs-137, and Eu-152 similar for both species. Concentrations of artificial radionuclides in biomass of shining pondweed and water moss correlated with annual discharges of the radionuclides to the Yenisei at different levels of significance, and the strongest (R-2 0.7) positive correlation (p 0.05) was obtained for Co-60, Zn-65, and Eu-152. Concentrations of Co-60, Cs-137, and Eu-152 in water moss were 2-7.5 times higher than in shining pondweed, and considerable percentages of those isotopes were recorded in extracellular particulate matter, which was largely represented by epiphytic diatoms. Higher concentrations of artificial radionuclides in the biomass of water moss can be considered as an advantage of water moss as a monitor of radioactive contamination of the Yenisei, while shining pondweed is more useful for estimation of annual deposits of radionuclides in vegetation of the Yenisei and spatial transfer of radionuclides downstream of the discharge site. Despite differences in concentrations of artificial radionuclides, both species can be considered as reliable indicators of radioactive contamination of the river on a long-term scale.
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