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Estimation of transfer of technogenic radionuclides in food chains of the Yenisei river / E. A. Trofimova, T. A. Zotina, A. Y. Bolsunovskii // Contemporary Problems of Ecology. - 2012. - Vol. 5, Is. 4. - P365-370, DOI 10.1134/S1995425512040142 . - ISSN 1995-4255
Кл.слова (ненормированные):
burbot -- dace -- grayling -- pike -- water moss -- zoobenthos -- bioaccumulation -- food chain -- gadoid -- gamma ray radiation -- macrophyte -- moss -- radioactive pollution -- radionuclide -- salmonid -- trophic level -- zoobenthos -- Russian Federation -- Yenisei River -- Bryophyta -- Esocidae -- Lota
Аннотация: The efficiency of the transfer of gamma-emitting radionuclides in food chains, including macrophytes, zoobenthos, and bentho- and ichthyophagist fish, was estimated in the radiation-polluted region of the Yenisei river. Significant differences in the efficiency of the transfer of natural and technogenic radionuclides between components of different trophic levels were revealed. Substantial species-related differences in the accumulation of radionuclides in ichthyophagist fish from food were revealed. В© 2012 Pleiades Publishing, Ltd.

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Держатели документа:
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Trofimova, E.A.; Zotina, T.A.; Bolsunovskii, A.Y.

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Assessment of the rate of accumulation of the transuranium element americium-241 by the aquatic plant Elodea canadensis / A. Ya. Bolsunovskii [et al.] // Doklady Biological Sciences. - 2004. - Vol. 399, Is. 1-6. - P467-469, DOI 10.1007/s10630-005-0014-0 . - ISSN 0012-4966
Кл.слова (ненормированные):
americium -- radioisotope -- water -- article -- biomass -- drug effect -- environmental monitoring -- Hydrocharitaceae -- methodology -- pH -- plant -- plant root -- radiation exposure -- radiochemistry -- time -- toxicity testing -- water pollutant -- Americium -- Biomass -- Environmental Monitoring -- Hydrocharitaceae -- Hydrogen-Ion Concentration -- Plant Roots -- Plant Shoots -- Plants -- Radiochemistry -- Radioisotopes -- Time Factors -- Toxicity Tests -- Water -- Water Pollutants, Radioactive -- Elodea canadensis

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Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovskii, A.Ya.; Zotina, T.A.; Bondareva, L.G.; Degermendzhi, A.G.

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Accumulation of 60Co, 90Sr, 137Cs, 238U and transuranic elements by berry shrubs in forest ecosystems of the Yenisei river basin / D. Dementyev, A. Bolsunovsky // Radioprotection. - 2011. - Vol. 46, Is. 6 SUPPL. - S25-S28, DOI 10.1051/radiopro/20116476s . - ISSN 0033-8451
Аннотация: The study investigates accumulation of radionuclides in 5 species of berry shrubs in forests of the Yenisei River basin (the Krasnoyarskii Krai, Russia). Activity concentrations of 60Co, 90Sr, 137Cs, 238U and transuranic elements in aboveground organs of the shrubs have been determined and their transfer factors calculated. Analysis of the calculated TFs shows that shrubs concentrate 90Sr more effectively than other study elements (TF = 0.25-1.9). The highest concentration of 137Cs is recorded in the berries and leaves of Ribes nigrumand Rubus idaeus. For these species, the range of 137Cs TFs (0.006-0.027) overlaps with the range of 238U TFs (0.004-0.018). For the aboveground parts of Ribes nigrum, TFs for transuranic radionuclides (0.01-0.056) are similar to or higher than the TFs for 238U and 137Cs. В© 2011 EDP Sciences.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Dementyev, D.; Bolsunovsky, A.

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Chemical fractionation of radionuclides and stable elements in aquatic plants of the yenisei river / A. Bolsunovsky // Environmental Science and Technology. - 2011. - Vol. 45, Is. 17. - P7143-7150, DOI 10.1021/es2008853 . - ISSN 0013-936X
Кл.слова (ненормированные):
Aquatic plants -- Artificial radionuclides -- Cell structure -- Chemical Fractionation -- Distribution patterns -- Nuclear plant -- Plant biomass -- Sequential extraction scheme -- Stable elements -- Submerged plants -- Transuranic elements -- Americium -- Biomass -- Neptunium -- River pollution -- Rivers -- Radioisotopes -- americium -- element -- neptunium -- radioisotope -- bioaccumulation -- chemical binding -- gamma ray spectrometry -- isotopic fractionation -- monocotyledon -- phytomass -- radionuclide -- river pollution -- submerged vegetation -- aquatic species -- article -- Batrachium kauffmanii -- biomass -- cell structure -- controlled study -- extraction -- Fontinalis antipyretica -- fractionation -- gamma spectrometry -- higher plant -- nonhuman -- plant growth -- Potamogeton lucens -- Potamogetonaceae -- river -- Russian Federation -- Aquatic Organisms -- Biomass -- Chemical Fractionation -- China -- Industrial Waste -- Plants -- Radiation Monitoring -- Radioisotopes -- Rivers -- Water Pollutants, Radioactive -- Russian Federation -- Yenisei River -- Batrachium -- Fontinalis antipyretica -- Potamogeton lucens
Аннотация: The Yenisei River is contaminated with artificial radionuclides released by one of the Russian nuclear plants. The aquatic plants growing in the radioactively contaminated parts of the river contain artificial radionuclides. The aim of the study was to investigate accumulation of artificial radionuclides and stable elements by submerged plants of the Yenisei River and estimate the strength of their binding to plant biomass by using a new sequential extraction scheme. The aquatic plants sampled were: Potamogeton lucens, Fontinalis antipyretica, and Batrachium kauffmanii. Gamma-spectrometric analysis of the samples of aquatic plants has revealed more than 20 radionuclides. We also investigated the chemical fractionation of radionuclides and stable elements in the biomass and rated radionuclides and stable elements based on their distribution in biomass. The greatest number of radionuclides strongly bound to biomass cell structures was found for Potamogeton lucens and the smallest for Batrachium kauffmanii. For Fontinalis antipyretica, the number of distribution patterns that were similar for both radioactive isotopes and their stable counterparts was greater than for the other studied species. The transuranic elements 239Np and 241Am were found in the intracellular fraction of the biomass, and this suggested their active accumulation by the plants. В© 2011 American Chemical Society.

Scopus
Держатели документа:
Institute of Biophysics SB, Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.

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Bioavailability of 99Tc to a macrophyte of the Yenisei River / L. Bondareva, A. Bolsunovsky // Radioprotection. - 2009. - Vol. 44, Is. 5. - P71-76, DOI 10.1051/radiopro/20095019 . - ISSN 0033-8451
Аннотация: The experiments on accumulation of 99Tc by Elodea biomass showed that 99Tc activity concentration can reach 120 В± 6Bq/g, with the concentration factor for 99Tc 2700 В± 500 L/kg. In experiments on 99Tc release, over 504 h about 82% of the total 99Tc activity was released into the water from plant biomass; most of 99Tc was released within the first 192 h. Results of chemical fractionation of the biomass show that 99Tc contained in biomass was mainly concentrated in the exchangeable and the adsorbed fractions (83%). Thus, the data obtained using chemical fractionation of biomass confirmed the experimental data on 99Tc release, which suggested that most of the biomass-bound 99Tc was adsorbed on the biomass surface. 99Tc tightly bound to Elodea biomass (fractions of organics and mineral residue) constituted just 17% of the total 99Tc activity. Decreased illumination of Elodea shoots during the experiment did not cause any reduction in 99Tc activity concentration or concentration factor. Results of chemical fractionation of the biomass grown under lower illumination conditions show that the percentage of 99Tc tightly bound to Elodea biomass (fractions of organics and mineral residue) decreased while the 99Tc of the adsorbed fractions decreased. Our results and data reported by other authors suggest that some part of 99Tc activity can be bioavailable to living organisms and that the portion of bioavailable 99Tc can be determined by a number of factors. В© 2009 EDP Sciences.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bondareva, L.; Bolsunovsky, A.

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Accumulation of 242Pu by a macrophyte of the Yenisei River (Elodea canadensis) in laboratory experiments / A. Bolsunovsky [et al.] // Chemosphere. - 2009. - Vol. 75, Is. 3. - P284-288, DOI 10.1016/j.chemosphere.2008.12.036 . - ISSN 0045-6535
Кл.слова (ненормированные):
242Pu -- Accumulation -- Laboratory experiments -- Sequential extraction technique -- Submerged plant Elodea canadensis -- Yenisei River -- sup242/supPu -- Accumulation -- Laboratory experiments -- Sequential extraction technique -- Submerged plant Elodea canadensis -- Yenisei River -- Biological materials -- Biomass -- Chemical plants -- Experiments -- Positive ions -- Rivers -- Plutonium -- concentration (composition) -- experimental study -- laboratory method -- macrophyte -- plutonium isotope -- river water -- submerged vegetation -- Eurasia -- Russian Federation -- Yenisei River -- plutonium -- article -- biomass -- chemistry -- Hydrocharitaceae -- physiology -- plant -- radiation monitoring -- river -- time -- water pollutant -- Biomass -- Hydrocharitaceae -- Plant Shoots -- Plutonium -- Radiation Monitoring -- Rivers -- Time Factors -- Water Pollutants, Radioactive
Аннотация: The study addresses 242Pu accumulation by Elodea canadensis, one of the abundant species of submerged plants in the Yenisei River. 242Pu in water samples of the "Elodea - Yenisei River water" model system and in the biomass fractions was determined ?-spectrometrically, following radiochemical recovery of 242Pu using 236Pu - a chemical yield tracer. The experiments on accumulation of 242Pu by Elodea biomass showed that the activity concentration of 242Pu can reach 21 В± 2 Bq/g dry wt, with the concentration factor for 242Pu 13100 В± 2100 L/kg dry wt. Results of chemical fractionation proved that during the first few hours of the experiment 242Pu contained in Elodea was mainly concentrated in the exchangeable and the adsorbed fractions of biomass (about 100%). As Elodea biomass accumulated 242Pu, the absolute amount of 242Pu in the exchangeable and the adsorbed fractions remained almost unchanged, although the portion of 242Pu tightly bound to biomass increased. At the end of the experiment, on day 7, 242Pu tightly bound to biomass (fractions of organics and mineral residue) constituted 43-63% (in different experiments) of the total 242Pu in the biomass. В© 2008 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, the Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Institute of Geology and Mineralogy, Siberian Branch, the Russian Academy of Sciences, Novosibirsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.; Bondareva, L.; Sukhorukov, F.; Melgunov, M.

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Accumulation and release of 99Tc by a macrophyte of the Yenisei River (Elodea canadensis) in laboratory experiments / A. Bolsunovsky, L. Bondareva // Journal of Radioanalytical and Nuclear Chemistry. - 2008. - Vol. 277, Is. 3. - P631-636, DOI 10.1007/s10967-007-7148-5 . - ISSN 0236-5731
Кл.слова (ненормированные):
technetium 99m -- aquatic flora -- article -- biomass -- controlled study -- dry weight -- liquid scintillation counting -- macrophyte -- nonhuman -- radiation absorption -- radiation detection -- radiation dose fractionation -- radiation measurement -- radioactivity -- river -- water sampling
Аннотация: The study addresses 99Tc accumulation and release by Elodea canadensis, one of the abundant species of submerged plants in the Yenisei River. 99Tc in water samples of the "Elodea - Yenisei River water" model system and in the biomass fractions was measured using a liquid scintillation analyzer. Experiments on accumulation of 99Tc by Elodea showed that 99Tc activity concentration can reach 120В±6 Bq/g dry wt, with the concentration factor for 99Tc 2700В±500 l/kg dry wt. In experiments on 99Tc release, over 504 hours about 82% of the total 99Tc activity was released into the water from the plant; most of 99Tc was released within the first 192 hours. The data obtained using sequential chemical fractionation of biomass confirmed the experimental data on 99Tc release, which suggested that most of the biomass-bound 99Tc was adsorbed on the surface of Elodea. 99Tc tightly bound to biomass (fractions of organics and mineral residue) constituted just 17% of the total 99Tc activity. В© 2008 Akademiai Kiado, Budapest.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.; Bondareva, L.

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Actinides and other radionuclides in sediments and submerged plants of the Yenisei River / A. Bolsunovsky, L. Bondareva // Journal of Alloys and Compounds. - 2007. - Vol. 444-445, Is. SPEC. ISS. - P495-499, DOI 10.1016/j.jallcom.2007.01.146 . - ISSN 0925-8388
Кл.слова (ненормированные):
Accumulation -- Actinides in aquatic plants and sediments -- Radiochemical and ?-spectrometric investigations -- Sequential extraction technique -- Yenisei River -- Actinides -- Bioaccumulation -- Plants (botany) -- Radioisotopes -- Rivers -- Sampling -- Sediments -- Mining-and-Chemical combine (MCC) -- Radiochemical and spectrometric investigations -- Sediment samples -- Sequential extraction techniques -- Contamination
Аннотация: The source of radioactive contamination of the Yenisei River floodplain, including contamination with actinides, is the Mining-and-Chemical combine (MCC), which has for many years been producing weapons-grade plutonium. Actinides have been detected not only in the soil and sediment of the river but also in the biomass of aquatic plants. The aim of our investigation was to assess the levels of actinides and other radionuclides in sediments and aquatic plants both near the MCC and at a considerable distance from it, down the Yenisei River. Investigations of the Yenisei River sediment samples revealed high activity concentrations of actinides (Pu isotopes and 241Am), which were 100 times higher than their global fallout levels. Sequential extraction of radionuclides from samples of sediments collected near the MCC showed that the amounts of extracted 241Am were the largest (up to 98% of initial activity). It was found that aquatic plants of the Yenisei River collected both near the MCC discharge site and at a distance up to 200 km downstream contained several actinide isotopes. The aquatic moss, Fontinalis antipyretica, was found to contain higher levels of radionuclides than Potamogeton lucens. Leaves of P. lucens contained higher levels of radionuclides, including 239Np, than stems. Sequential extraction of radionuclides from samples of aquatic plants showed that 239Np levels in exchangeable and adsorption fractions of P. lucens biomass were higher than in the respective fractions of F. antipyretica biomass. В© 2007 Elsevier B.V. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, the Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.; Bondareva, L.

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Accumulation and release of 241Am by a macrophyte of the Yenisei River (Elodea canadensis) / A. Bolsunovsky, T. Zotina, L. Bondareva // Journal of Environmental Radioactivity. - 2005. - Vol. 81, Is. 1. - P33-46, DOI 10.1016/j.jenvrad.2004.10.012 . - ISSN 0265-931X
Кл.слова (ненормированные):
241Am -- Accumulation -- Laboratory experiments -- Release -- Submerged plant Elodea canadensis -- Yenisei River -- Activation analysis -- Biomass -- Concentration (process) -- Effluents -- Plutonium -- Rivers -- Aquatic plants -- Elodea plant -- Macrophytes -- Radioactive contamination -- Transuranium elements -- americium 241 -- river water -- americium -- americium nitrate -- bioaccumulation -- biological uptake -- macrophyte -- pollutant source -- radioactive pollution -- river water -- submerged vegetation -- article -- biomass -- concentration (parameters) -- environmental factor -- fractionation -- laboratory -- macrophyte -- radioactivity -- river -- sampling -- adsorption -- chemistry -- Hydrocharitaceae -- methodology -- physiology -- plant -- radiation exposure -- radiation monitoring -- Russian Federation -- sediment -- soil pollutant -- time -- tissue distribution -- water pollutant -- Eastern Hemisphere -- Eurasia -- Russian Federation -- World -- Yenisei River -- Elodea canadensis -- Adsorption -- Americium -- Biomass -- Chemical Fractionation -- Geologic Sediments -- Hydrocharitaceae -- Plant Shoots -- Radiation Monitoring -- Rivers -- Russia -- Soil Pollutants, Radioactive -- Time Factors -- Tissue Distribution -- Water Pollutants, Radioactive
Аннотация: The source of radioactive contamination of the Yenisei River floodplain, including contamination with transuranic elements, is the Mining-and-Chemical Combine of the Russian Ministry of Atomic Energy, which has for many years been producing weapons-grade plutonium. Transuranic elements have been detected not only in the soil and sediment of the river but also in the biomass of aquatic plants. This work is an investigation of accumulation and release of 241Am by a submerged macrophyte of the Yenisei River (Elodea canadensis) in laboratory experiments. In 2000-2003, laboratory experiments were carried out with biomass of E. canadensis Mich. and filtered river water. The samples were collected from the Yenisei River upstream of the discharge of the Combine's radioactive effluent. The experiments showed that 241Am is accumulated by Elodea biomass: the activity concentration of 241Am can reach 3280 В± 240 Bq/g, with the concentration factor for 241Am 16 600 В± 2200 l/kg. Results of chemical fractionation have proved that in the course of 241Am accumulation by Elodea biomass, 241Am tightly bound to biomass increases from 11% to 27% of the total 241Am in the biomass. Release of 241Am from the decaying Elodea biomass has been evaluated experimentally. By the end of the experiment (lasting up to 127 days), the Elodea plants had lost up to 65% of their initial 241Am activity and the rate of 241Am release into the water environment reached 23 Bq/day. В© 2004 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.; Zotina, T.; Bondareva, L.

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10.

Artificial radionuclides in aquatic plants of the Yenisei River in the area affected by effluents of a Russian plutonium complex / A. Bolsunovsky // Aquatic Ecology. - 2004. - Vol. 38, Is. 1. - P57-62, DOI 10.1023/B:AECO.0000020950.43944.ec . - ISSN 1386-2588
Кл.слова (ненормированные):
?-radionuclides -- Aquatic plants -- Concentration factor -- Isotopes of plutonium -- aquatic plant -- bioaccumulation -- effluent -- radionuclide -- Eurasia -- Russian Federation -- Yenisei River -- Bryophyta -- Fontinalis -- Fontinalis antipyretica -- Potamogeton -- Potamogeton lucens
Аннотация: The Yenisei River, one of the largest rivers in the world, is contaminated with artificial radionuclides released by one of the Russian nuclear plants, which produces weapons-grade plutonium and has been in operation for many years. The aim of the study that was conducted between 1997 and 2002 was to investigate accumulation of artificial radionuclides by aquatic plants of the Yenisei River. The aquatic plants sampled were: Potamogeton lucens (shining weed) and Fontinalis antipyretica (water moss). The ?-spectrometric analysis of the samples of aquatic plants for artificial radionuclides has revealed a wide spectrum of long-lived and short-lived radionuclides. Artificial radionuclides such as 51Cr, 54Mn, 58Co, 60Co, 65Zn, 137Cs, and 152Eu were found in aquatic plants collected both near the plutonium complex and 194 km downstream in the river. The radiochemical analysis of aquatic plants revealed strontium and isotopes of plutonium. Fontinalis antipyretica had very high concentration factors of the principal radionuclides: 14220, 3110 and 500 of 51Cr, 46Sc and 239Np, respectively.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.

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11.

First data on technetium-99 content in the ecosystem of the Yenisei River / A. Ya. Bolsunovskii, Yu. V. Aleksandrova, A. G. Degermendzhi // Doklady Earth Sciences. - 2010. - Vol. 434, Is. 1. - P1219-1221, DOI 10.1134/S1028334X10090163 . - ISSN 1028-334X
Кл.слова (ненормированные):
Aquatic plants -- Concentration factors -- Dumping site -- Filtered water -- Laboratory conditions -- Maximal values -- Radioactive effluents -- Technetium 99 -- Ecology -- Effluents -- Sewage -- Technetium -- Wastewater -- Water content -- aquatic plant -- bioaccumulation -- freshwater ecosystem -- radioactive pollution -- river water -- technetium isotope -- Russian Federation -- Yenisei River -- Potamogeton lucens
Аннотация: The content of a long-lived technogenic radionuclide 99Tc in the Yenisei within the territory of radioactive effluents of the Rosatom mining chemical combine (MCC) has been studied. In 2008-2009 the maximal 99Tc content in water was registered near the dumping site of the MCC and came to 33 mBq/l. According to analysis of filtered water samples, technetium in the Yenisei water is presented as pertechnetate ion (TcO 4). When moving away from the MCC downstream, the 99Tc content in water is decreased, which is caused both by dilution of waste water and by 99Tc accumulation by aquatic plants. The 99Tc content in the biomass of the aquatic plant Potamogeton lucens came to 82 15 Bq/kg, and the concentration factor (CF) to 8200, which is three times more than the maximal values of the CF of 99Tc by the plants in laboratory conditions (2700). В© 2010 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovskii, A.Ya.; Aleksandrova, Yu.V.; Degermendzhi, A.G.

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12.

Ecology of purple sulfur bacteria in the highly stratified meromictic Lake Shunet (Siberia, Khakassia) in 2002-2009 / D. Y. Rogozin, V. V. Zykov, A. G. Degermendzhi // Microbiology (Russian Federation). - 2012. - Vol. 81, Is. 6. - P727-735, DOI 10.1134/S0026261712060148 . - ISSN 0026-2617
Кл.слова (ненормированные):
bacteriochlorophyll a -- chemocline -- meromictic lake -- purple sulfur bacteria -- seasonal dynamics -- Bacteria (microorganisms) -- Chromatiaceae
Аннотация: Phototrophic sulfur bacteria form dense accumulations in the chemocline zones of stratified lakes where light reaches the sulfide-containing layers of water. Many works are dedicated to the ecophysiology of these microorganisms in meromictic lakes. However, the role of these microorganisms in the trophic network of these ecosystems, the ways of biomass utilization, and the contribution to the turnover of biogenic elements have so far been insufficiently understood. This work deals with the analysis of many years' seasonal dynamics of the biomass of purple sulfur bacteria and the physicochemical conditions of their environment in Lake Shunet (Siberia, Khakassia, Russia), unraveling the causes of their anomalous development in the chemocline of this lake, as well as the comparative analysis of such type of ecosystems. Lake Shunet is characterized by markedly pronounced stratification and the high density of purple sulfur bacteria (PSB) in the chemocline, which is comparable to that of Lake Mahoney (Canada) where the number of PSB is the greatest among those known in the world. It was shown that, in the period 2002-2009, the total amount of bacterio-chlorophyll a in the water column of Lake Shunet increased and did not correlate with the seasonal variations in temperature and illumination in the chemocline. It was established that PSB cells in the purple layer experienced the effect of self-shading. The sedimentation rate of purple sulfur bacteria in Lake Shunet was low due to the pronounced density gradient in the chemocline zone. Thus, the high number of PSB in the chemocline was due to the combination of strong illumination, a high sulfide concentration, and a high water density gradient, which was responsible for stable stratification and contributed to the accumulation of the cells in a narrow layer. The data obtained could be useful for the paleoreconstruction of climatically deter-mined changes in the level of the lake and its periods of meromixis by the presence of carotenoids and bacte-riochlorophylls in the bottom sediments. В© 2012 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Rogozin, D.Y.; Zykov, V.V.; Degermendzhi, A.G.

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13.

Principle of the worst scenario in the modelling past and future of biosphere dynamics / S. I. Bartsev, A. G. Degermendzhi, D. V. Erokhin // Ecological Modelling. - 2008. - Vol. 216, Is. 2. - P160-171, DOI 10.1016/j.ecolmodel.2008.03.002 . - ISSN 0304-3800
Кл.слова (ненормированные):
Irreversible biosphere changes -- Minimal model of biosphere -- The worst scenario principle -- Biospherics -- agricultural land -- anthropogenic effect -- atmosphere-biosphere interaction -- carbon dioxide -- deforestation -- Little Ice Age -- numerical model -- Eurasia -- Europe
Аннотация: The "biosphere-climate" system is subjected to different influences (influx of anthropogenic CO2 and pollutants, deforestation, harmful land management, biological species depopulation, etc.). Therefore, the vital question arises: "Can these influences lead to irreversible negative changes in the climate-biosphere system or a global ecological catastrophe?" The possibility of irreversible changes may be not very high, but one cannot ignore it. So the main aim of our investigation is to evaluate possible consequences of human impact on the biosphere focusing on irreversible changes of it. Traditional mathematical complicated models describe the biosphere in great detail, but the large number of equations and parameters leads to accumulation of uncertainties in the forecast due to inevitable uncertainties of experimental estimations of model parameters. An approach based on the principle of the worst scenario was proposed. Minimization of mathematical model with respect to this principle is conducted to study limiting (but possible) versions of models in which the contribution of the possible compensatory and smoothing mechanisms is minimal. Namely the most unfavorable scenarios (corresponding to the values of parameters at the boundaries of confidence interval) have to be considered in estimating consequences of anthropogenic impact. One of the fastest CO2 releasing mechanisms, based on the positive feedback effect, was considered in the context of the worst scenario principle. The family of simple mathematical models was created for biosphere dynamics representation on different timescales. The main result of the investigation consists in confirmation of the possibility of negative and irreversible changes in the "biosphere-climate" system, caused by amplification of the positive feedback: "anthropogenic emission of CO2 - temperature increase - additional CO2 emission due to decomposition of soil organics". Crucial parameters of models, responsible for avalanche-like biosphere changes, are determined. A realizability of hypotheses on anthropogenic causes of Little Ice Age was estimated by corresponding minimal model. Model were used to show the feasibility of the mechanism describing the changing of agricultural field species into indigenous forests in Europe during the Plague, which led to CO2 decrease and temperature fall. В© 2008 Elsevier B.V. All rights reserved.

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Держатели документа:
Institute of Biophysics SB RAS, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bartsev, S.I.; Degermendzhi, A.G.; Erokhin, D.V.

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14.

An estimate of 241Am accumulation by the macrophyte Elodea Canadensis / A. Ya. Bolsunovskij [и др.] // Doklady Akademii Nauk. - 2004. - Vol. 399, Is. 1. - С. 130-133 . - ISSN 0869-5652
Кл.слова (ненормированные):
Biomass -- Fractionation -- Minerals -- Organic compounds -- Plants (botany) -- Radioactivity -- Radioisotopes -- Accumulation -- Macrophytes -- Americium
Аннотация: The lab experiments showed that specific activity of 241Am achieves 3280В±240 Bc/g with 241Am accumulation coefficient = 16600В±2200 l/kg. The chemical fractionation results confirmed that 241Am can be strongly fixed by elodea biomass. At the end of long-term experiment the part of 241Am strongly bonded by the biomass reached 27% of total 241Am content in biomass. The accumulation process proceeds with 241Am redistribution towards decreasing of the part of exchange and adsorbed fractions and increasing of the part of organic and mineral fractions. Thus, the 241Am part in organic fractions doubled during the experiment and in mineral fraction - grew more than 10 times as compared with initial 241Am distribution.

Scopus
Держатели документа:
Inst. Biofiziki SO RAN, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovskij, A.Ya.; Zotina, T.A.; Bondareva, L.G.; Degermendzhi, A.G.

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15.

Accumulation of artificial radionuclides by the Yenisei river aquatic plants in the area affected by the activity of the Mining-and-Chemical Combine / A. Ya. Bolsunovskij [и др.] // Radiatsionnaya Biologiya. Radioekologiya. - 2002. - Vol. 42, Is. 2. - С. 194-199 . - ISSN 0869-8031
Кл.слова (ненормированные):
Contamination -- Ecosystems -- Environmental impact -- Gamma ray spectrometers -- Radioisotopes -- River pollution -- Radioecological concentration -- Radioisotope accumulation -- The Yenisei river -- Ecology -- plutonium -- radioisotope -- strontium -- article -- chemical industry -- chemistry -- comparative study -- gamma spectrometry -- industrial waste -- mining -- nuclear reactor -- plant -- radiochemistry -- Russian Federation -- water pollutant -- Chemical Industry -- Industrial Waste -- Mining -- Nuclear Reactors -- Plants -- Plutonium -- Radiochemistry -- Radioisotopes -- Siberia -- Spectrometry, Gamma -- Strontium Radioisotopes -- Water Pollutants, Radioactive
Аннотация: The aim of the paper is to investigate accumulation of artificial radionuclides by the Yenisei river aquatic plants collected in the area affected by the activity of the Mining-and-Chemical Combine (Zheleznogorsk) from 1997 to 2000. The samples of aquatic plants were of four species: Potamogeton lucens, Fontinalis antipyretica, Elodea canadensis, and Ceratophyllum demersum. The gamma-spectrometric analysis of the samples of aquatic plants for artificial radionuclides revealed a wide spectrum of radionuclides. Radionuclides of activation origin were found in the aquatic plants taken both near the Combine and 200 km down of it. The radiochemical analysis of aquatic plants revealed strontium and plutonium isotopes. Among the aquatic plants, the highest concentration factors for the principal radionuclides were recorded in Fontinalis antipyretica (water moss).

Scopus
Держатели документа:
Inst. of Biophysics, Siberian Branch, RAS, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovskij, A.Ya.; Ermakov, A.I.; Burger, M.; Degermendzhy, A.G.; Sobolev, A.I.

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16.

Cell growth and accumulation of polyhydroxyalkanoates from CO2 and H2 of a hydrogen-oxidizing bacterium, Cupriavidus eutrophus B-10646 / T. G. Volova [et al.] // Bioresource Technology. - 2013. - Vol. 146. - P215-222, DOI 10.1016/j.biortech.2013.07.070 . - ISSN 0960-8524
Кл.слова (ненормированные):
Autotrophic synthesis -- Cupriavidus eutrophus -- Polyhydroxyalkanoates
Аннотация: Synthesis of polyhydroxyalkanoates (PHAs) by a new strain of Cupriavidus - Cupriavidus eutrophus B-10646 - was investigated under autotrophic growth conditions. Under chemostat, at the specific flow rate D=0.1h-1, on sole carbon substrate (CO2), with nitrogen, sulfur, phosphorus, potassium, and manganese used as growth limiting elements, the highest poly(3-hydroxybutyrate) [P(3HB)] yields were obtained under nitrogen deficiency. In batch autotrophic culture, in the fermenter with oxygen mass transfer coefficient 0.460h-1, P(3HB) yields reached 85% of dry cell weight (DCW) and DCW reached 50g/l. Concentrations of supplementary PHA precursor substrates (valerate, hexanoate, ?-butyrolactone) and culture conditions were varied to produce, for the first time under autotrophic growth conditions, PHA ter- and tetra-polymers with widely varying major fractions of 3-hydroxybutyrate, 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate monomer units. Investigation of the high-purity PHA specimens showed significant differences in their physicochemical and physicomechanical properties. В© 2013 Elsevier Ltd.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
L.V. Kirenckii Institute of Physics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Institute of Chemistry and Chemical Technology of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kiselev, E.G.; Shishatskaya, E.I.; Zhila, N.O.; Boyandin, A.N.; Syrvacheva, D.A.; Vinogradova, O.N.; Kalacheva, G.S.; Vasiliev, A.D.; Peterson, I.V.

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17.

Effects of intracellular poly(3-hydroxybutyrate) reserves on physiological-biochemical properties and growth of Ralstonia eutropha / T. G. Volova [et al.] // Research in Microbiology. - 2013. - Vol. 164, Is. 2. - P164-171, DOI 10.1016/j.resmic.2012.10.008 . - ISSN 0923-2508
Кл.слова (ненормированные):
Granules -- PHA cycle -- Poly(3-hydroxybutyrate) -- Ralstonia eutropha -- poly(3 hydroxybutyric acid) -- article -- bacterial growth -- bacterial strain -- bacterium culture -- cell division -- cell granule -- cell size -- controlled study -- Cupriavidus necator -- electron microscopy -- nonhuman -- polymer production -- priority journal -- Cupriavidus necator -- Cytoplasmic Granules -- Hydroxybutyrates -- Microscopy, Electron, Transmission -- Polyesters -- Bacteria (microorganisms) -- Cupriavidus necator
Аннотация: Microbial polyhydroxyalkanoates (PHAs), because of their well studied complex physiology and commercial potential, are vehicles for carbon and potential storage reduction for many microbial species. Even with the wealth of studies about microbial PHAs in the scientific literature, polymer accumulation and degradation are still not comprehensively understood. Poly(3-hydroxybutyrate) (P3HB) granule formation and polymer mobility were studied here in the bacterium Ralstonia eutropha strain B5786 in autotrophic cultures. Electron microscopy studies revealed decreasing cell size concomitant with enlargement of size and number of intracellular granules, and inhibition of cell division during intracellular polymer production. Activities of key P3HB biosynthetic enzymes demonstrated correlations with each other during polymer accumulation, suggesting an intricately regulated P3HB cycle in autotrophically grown R. eutropha cells. В© 2012 .

Scopus
Держатели документа:
Laboratory of Chemoautotrophic Biosynthesis, Institute of Biophysics of the Siberian Branch of The Russian Academy of Sciences, Akademgorodok 50, Krasnoyarsk 660036, Russian Federation
Department of Biotechnology, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Avenue, Krasnoyarsk 660041, Russian Federation
Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge 02139, MA, United States
Health Sciences Technology Division, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge 02139, MA, United States
Engineering Systems Division, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge 02139, MA, United States : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Zhila, N.O.; Kalacheva, G.S.; Brigham, C.J.; Sinskey, A.J.

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18.

Distribution and resorption of polymeric microparticles in visceral organs of laboratory animals after intravenous injection / E. I. Shishatskaya [et al.] // Bulletin of Experimental Biology and Medicine. - 2009. - Vol. 148, Is. 5. - P789-793, DOI 10.1007/s10517-010-0817-3 . - ISSN 0007-4888
Кл.слова (ненормированные):
14C -- Intravenous injection -- Microparticles -- Resorbable polyhydroxybutyrate -- Visceral tissues -- carbon 14 -- microsphere -- polyhydroxyaminobutyric acid -- polymer -- unclassified drug -- animal experiment -- animal tissue -- article -- biocompatibility -- biodegradability -- body growth -- controlled drug release -- controlled study -- degradation kinetics -- drug delivery system -- drug dosage form -- experimental animal -- female -- liver parenchyma -- molecular weight -- nonhuman -- particle size -- physical development -- radioactivity -- rat -- spleen -- tissue distribution -- tissue structure -- Animals -- Animals, Laboratory -- Carbon Radioisotopes -- Female -- Injections, Intravenous -- Particle Size -- Polymers -- Rats -- Rats, Wistar -- Tissue Distribution -- Viscera -- Animalia
Аннотация: Microparticles obtained by using 14C-labeled resorbable hydroxyaminobutyric acid polymer were injected into the caudal vein of laboratory animals without negative aftereffects for their growth and development and without changes in the macro- and microstructure of organs and tissues. The distribution of microparticles in the viscera and the dynamics of accumulation of carbon-containing polymer degradation products in the viscera were studied. The main targets for the particles are liver tissues, as well as renal and splenic tissues. The polymeric matrix of the microparticles is most actively destroyed in the spleen and liver. The presence of high-molecular- weight polymeric matrix in organs indicates the integrity of microparticles and the possibility of long-term (up to 12 weeks) functioning of polymeric particles in vivo. В© 2009 Springer Science+Business Media, Inc.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Institute of Basic Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Goreva, A.V.; Voinova, O.N.; Kalacheva, G.S.; Volova, T.G.

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19.

Physiological-biochemical properties and the ability to synthesize polyhydroxyalkanoates of the glucose-utilizing strain of the hydrogen bacterium Ralstonia eutropha B8562 / T. G. Volova [et al.] // Applied Microbiology and Biotechnology. - 2006. - Vol. 73, Is. 3. - P429-433, DOI 10.1007/s00253-006-0460-0 . - ISSN 0175-7598
Кл.слова (ненормированные):
Biochemistry -- Carbon -- Genetic engineering -- Glucose -- Hydrogen -- Polyesters -- Cultural properties -- Glucose-utilizing mutant strain -- Nitrogen deficiency -- Polyhydroxyalkanoate (PHA) -- Bacteria -- 3 hydroxybutyric acid -- carbon dioxide -- fructose -- glucose -- hydrogen -- monomer -- polyhydroxyalkanoic acid -- sugar -- bacterium -- carbon -- comparative study -- energy -- ester -- experimental study -- glucose -- hydrogen -- physicochemical property -- article -- bacterial strain -- bacterium culture -- bacterium mutant -- biomass -- chemical analysis -- Cupriavidus necator -- energy consumption -- genetic analysis -- nonhuman -- synthesis -- Bacteria (microorganisms) -- Cupriavidus necator
Аннотация: Physiological-biochemical, genetic, and cultural properties of the glucose-utilizing mutant strain Ralstonia eutropha B8562 have been compared with those of its parent strain R. eutropha B5786. It has been shown that growth characteristics of the strain cultured on glucose as the sole carbon and energy source are comparable with those of the parent strain. Strain B8562 is characterized by high polyhydroxyalkanoate (PHA) yields on different carbon sources (CO2, fructose, and glucose). PHA accumulation in the strain batch cultured on glucose under nitrogen deficiency reaches 90 %. The major monomer in the PHA is ?-hydroxybutyric acid (more than 99 mol %); the identified minor components are ?-hydroxyvaleric acid (0.25-0.72 mol %) and ?-hydroxyhexanoic acid (0.08-1.5 mol %). The strain is a promising PHA producer on available sugar-containing media with glucose. В© Springer-Verlag 2006.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russian Federation
Krasnoyarsk State University, Svobodny av. 79, 660041 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Trusova, M.Y.; Kalacheva, G.S.; Kozhevnicov, I.V.

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20.

Physiological-biochemical properties and the ability to synthesize polyhydroxyalkanoates of the glucose-utilizing strain of the hydrogen bacterium Ralstonia eutropha B8562. / T. G. Volova [et al.] // Applied microbiology and biotechnology. - 2006. - Vol. 73, Is. 2. - P429-433, DOI 10.1007/s00253-006-0460-0 . - ISSN 0175-7598
Кл.слова (ненормированные):
beta hydroxyvaleric acid -- beta-hydroxyvaleric acid -- carbon dioxide -- ester -- fatty acid -- fructose -- glucose -- hexanoic acid derivative -- hydrogen -- hydroxyacid -- oxygen -- polymer -- ribosome RNA -- valeric acid derivative -- article -- biotechnology -- chemistry -- metabolism -- methodology -- Wautersia eutropha -- Biotechnology -- Carbon Dioxide -- Cupriavidus necator -- Esters -- Fatty Acids -- Fructose -- Glucose -- Hexanoic Acids -- Hydrogen -- Hydroxy Acids -- Oxygen -- Pentanoic Acids -- Polymers -- RNA, Ribosomal
Аннотация: Physiological-biochemical, genetic, and cultural properties of the glucose-utilizing mutant strain Ralstonia eutropha B8562 have been compared with those of its parent strain R. eutropha B5786. It has been shown that growth characteristics of the strain cultured on glucose as the sole carbon and energy source are comparable with those of the parent strain. Strain B8562 is characterized by high polyhydroxyalkanoate (PHA) yields on different carbon sources (CO(2), fructose, and glucose). PHA accumulation in the strain batch cultured on glucose under nitrogen deficiency reaches 90 %. The major monomer in the PHA is beta-hydroxybutyric acid (more than 99 mol %); the identified minor components are beta-hydroxyvaleric acid (0.25-0.72 mol %) and beta-hydroxyhexanoic acid (0.08-1.5 mol %). The strain is a promising PHA producer on available sugar-containing media with glucose.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Trusova, M.Y.; Kalacheva, G.S.; Kozhevnicov, I.V.

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