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Chlorophyll fluorescence induction and estimation of plant resistance to stress factors / T. V. Nesterenko, A. A. Tikhomirov, V. N. Shikhov // Zhurnal Obshchei Biologii. - 2007. - Vol. 68, Is. 6. - С. 444-458 . - ISSN 0044-4596
Кл.слова (ненормированные):
chlorophyll -- adaptation -- cucumber -- fluorescence -- growth, development and aging -- metabolism -- physiology -- plant leaf -- review -- Adaptation, Physiological -- Chlorophyll -- Cucumis sativus -- Fluorescence -- Plant Leaves -- Cucumis sativus
Аннотация: The usage of chlorophyll fluorescence induction (CFI) for estimating various types of plant resistance (primary, general, initial, adaptive) to stress factors is reviewed. The necessity of ontogenetic approach (considering the age-specific properties of the photosynthetic apparatus) in determining general and adaptive resistance of plants to prolonged action of stress factors by the CFI method is argued. In the plant Cucumbis sativus L., the possibility is shown of using age-specific qualitative and quantitative traits of leaf CFI (changes in the shape of chlorophyll fluorescence induction curves and in the dynamics of CFI parameters in the course of leaf ontogeny) for comparative study of differences between fully active and stressed plants. Possible criteria are suggested for estimating the effect of outer stress factors by the presence or absence of a steady-state phase in the dynamics of CFI parameters during leaf ontogeny. It is also suggested to use the duration of the steady-state phase following the termination of leaf growth (estimated by the dynamics of the slow phase of CFI as the ratio of fluorescence intensity at the peak P and the steady-state fluorescence intensity, FP/FS, or as the viability index Rfd) and the variability of CFI parameters during this period as qualitative estimates of plant resistance to prolonged action of stress factors.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Nesterenko, T.V.; Tikhomirov, A.A.; Shikhov, V.N.

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An ontogenetic approach to the assessment of plant resistance to stress factors based on the method of chlorophyll fluorescence induction. / T. V. Nesterenko, A. A. Tikhomirov // Doklady. Biochemistry and biophysics. - 2003. - Vol. 388. - P4-7 . - ISSN 1607-6729
Кл.слова (ненормированные):
chlorophyll -- adaptation -- aging -- article -- comparative study -- cucumber -- light -- metabolism -- methodology -- photostimulation -- photosynthesis -- physiology -- plant leaf -- radiation dose -- radiation exposure -- spectrofluorometry -- Adaptation, Physiological -- Aging -- Chlorophyll -- Cucumis sativus -- Light -- Photic Stimulation -- Photosynthetic Reaction Center Complex Proteins -- Plant Leaves -- Radiation Dosage -- Spectrometry, Fluorescence

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Nesterenko, T.V.; Tikhomirov, A.A.

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Tolerance of LSS plant component to elevated temperatures / S. A. Ushakova, A. A. Tikhomirov // Acta Astronautica. - 2002. - Vol. 50, Is. 12. - P759-764, DOI 10.1016/S0094-5765(02)00010-3 . - ISSN 0094-5765
Кл.слова (ненормированные):
Metabolism -- Photosynthesis -- Rate constants -- Thermal effects -- Thermoanalysis -- Thermal tolerance -- Life support systems (spacecraft) -- carbon dioxide -- adaptation -- article -- Brassicaceae -- comparative study -- heat -- instrumentation -- light -- metabolism -- microclimate -- photosynthesis -- physiology -- plant physiology -- wheat -- Adaptation, Physiological -- Brassicaceae -- Carbon Dioxide -- Ecological Systems, Closed -- Heat -- Life Support Systems -- Light -- Photosynthesis -- Plant Physiology -- Triticum
Аннотация: Stability of LSS based on biological regeneration of water, air and food subject to damaging factors is largely dependent on the behavior of the photosynthesizing component represented, mainly, by higher plants. The purpose of this study is to evaluate the tolerance of uneven-aged wheat and radish cenoses to temperature effects different in time and value. Estimation of thermal tolerance of plants demonstrated that exposure for 20 h to the temperature increasing to 45В°C brought about irreversible damage both in photosynthetic processes (up to 80% of initial value) and the processes of growth and development. Kinetics of visible photosynthesis during exposure to elevated temperatures can be used to evaluate critical exposure time within the range of which the damage of metabolic processes is reversible. With varying light intensity and air temperature it is possible to find a time period admissible for the plants to stay under adverse conditions without considerable damage of metabolic processes. В© 2002 Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics (Russian Academy of Sciences, Siberian Branch) Academgorodok, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Ushakova, S.A.; Tikhomirov, A.A.

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Experimental evaluation of the processes resulting from the introduction of the transgenic microorganism Escherichia coli Z905/pPHL7 (lux+) into aquatic microcosms / T. V. Kargatova [et al.] // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1769-1774, DOI 10.1016/S0273-1177(03)00119-4 . - ISSN 0273-1177
Кл.слова (ненормированные):
Escherichia coli -- Genes -- Sensitivity analysis -- Aquatic microcosms -- Space research -- genetically modified organism -- Adaptation, Physiological -- Ampicillin Resistance -- Animals -- Chemiluminescent Measurements -- Colony Count, Microbial -- Daphnia -- Ecological Systems, Closed -- Environmental Monitoring -- Escherichia coli -- Fresh Water -- Gene Expression Regulation, Bacterial -- Organisms, Genetically Modified -- Plasmids -- Protozoa -- Time Factors -- Water Microbiology
Аннотация: The processes resulting from the introduction of the transgenic microorganism (TM) E. coli Z905/pPHL7 into aquatic microcosms have been modeled experimentally. It has been shown that the TM E. coli is able to adapt to a long co-existence with indigenous heterotrophic microflora in variously structured microcosms. In more complex microcosms the numerical dynamics of the introduced E. coli Z905/pPHL7 population is more stable. In the TM populations staying in the microcosms for a prolonged time, changes are recorded in the phenotypic expression of plasmid genes (ampicillin resistance and the luminescence level) and chromosome genes (morphological and physiological traits). However, in our study microcosms, the recombinant plasmid persisted in the TM cells for 6 years after the introduction, and as the population adapts to the conditions of the microcosms, the efficiency of the cloned gene expression in the cells is restored. In the microcosms with high microalgal counts (107 cells/ml), cells with a high threshold of sensitivity to ampicillin dominate in the population of the TM E. coli Z905/pPHL7. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, SB RAS, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kargatova, T.V.; Boyandin, A.N.; Popova, L.Yu.; Pechurkin, N.S.

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Population dynamics of transgenic microorganisms in the different microecosystem conditions / L. Yu. Popova [et al.] // Advances in Space Research. - 2001. - Vol. 27, Is. 9. - P1571-1579, DOI 10.1016/S0273-1177(01)00248-4 . - ISSN 0273-1177
Кл.слова (ненормированные):
Ecosystems -- Escherichia coli -- Genes -- Interferons -- Luminescence -- Vaccines -- Microcosms -- Microorganisms -- fresh water -- microorganism -- adaptation -- article -- Bacillus subtilis -- comparative study -- ecosystem -- Escherichia coli -- gene expression -- genetics -- growth, development and aging -- hazardous waste -- microbiology -- Micrococcus -- osmotic pressure -- plasmid -- population dynamics -- risk assessment -- transgenic organism -- Adaptation, Physiological -- Bacillus subtilis -- Containment of Biohazards -- Ecosystem -- Escherichia coli -- Fresh Water -- Gene Expression -- Micrococcus -- Organisms, Genetically Modified -- Osmotic Pressure -- Plasmids -- Population Dynamics -- Risk Assessment -- Water Microbiology
Аннотация: The role of key environmental factors in adaptation of spore-forming and non-spore-forming transgenic microorganisms (TM) have been studied in model ecosystems. Model TM Escherichia coli Z905 (bearing plasmid genes of bacterial luminescence Ap1Lux+) has been found to have a higher adaptation potential than TM Bacillus subtilis 2335/105 (bearing genes of human ? 2-interferon Km1Inf+), planned for employment as a living vaccine under varying environmental conditions. Effects of abiotic factors on migration of natural and recombinant plasmids between microorganisms under model ecosystem conditions has been estimated. The transgenic microorganisms with low copy number survived better under introduction conditions in the microcosms studied. This trend has been shown to be independent of the microcosm type and its complexity. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Popova, L.Yu.; Lobova, T.I.; Krylova, T.Yu.; Kargatova, T.V.; Maksimova, E.E.; Boyandin, A.N.; Pechurkin, N.S.

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Life support system with autonomous control employing plant photosynthesis / I. I. Gitelson [et al.] // Acta Astronautica. - 1976. - Vol. 3, Is. 9-10. - P633-650 . - ISSN 0094-5765
Кл.слова (ненормированные):
BIOCHEMICAL ENGINEERING - Photosynthesis -- SPACECRAFT -- adaptation -- article -- Chlorella -- growth, development and aging -- human -- instrumentation -- intestine -- male -- metabolism -- microbiology -- microclimate -- photosynthesis -- physiology -- plant -- space flight -- task performance -- vegetable -- water supply -- wheat -- Adaptation, Physiological -- Chlorella -- Ecological Systems, Closed -- Environment, Controlled -- Humans -- Intestines -- Life Support Systems -- Male -- Metabolism -- Photosynthesis -- Plants -- Space Simulation -- Task Performance and Analysis -- Triticum -- Vegetables -- Water Supply
Аннотация: This research was aimed at obtaining a closed control system. This was achieved by placing all the technological processes providing for human vital activities within the hermetically sealed space, and by transferring the entire control and guidance of these processes to people inhabiting the system. In contrast to existing biological life support systems, man has been included not only as a participant of metabolism, but as an operator who is the central figure in collecting information, making decisions and controlling all technological processes. To tackle this problem, the "BIOS-3" experimental complex was created for performing long-term experiments using different structures of biological life-support system. The experiment lasted six months and consisted of three stages. During the first stage the system was comprised of two equivalent phytotrons with the culture of wheat and an assortment of vegetable plants, and the living compartment. At the second stage, one of the phytotrons was removed while a compartment of chlorella cultivators was introduced. The third stage differed from the second, the former using wheat phytotron and the latter employing phytotron with an assortment of vegetable cultures. Three men inhabited the system simultaneously. The experiment demonstrated that a biological life support system controlled autonomously from the inside is feasible within a small confined space. However, immunological and microbiological research shows, that the medium created by the system is not fully adequate for man. In conclusion, some prospects have been outlined for further studies of biological life support systems. В© 1976.

Scopus
Держатели документа:
L. V. Kirensky Physics Institute, Siberian Branch, USSR Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, I.I.; Terskov, I.A.; Kovrov, B.G.; Sidko, F.Ya.; Lisovsky, G.M.; Okladnikov, Yu.N.; Belyanin, V.N.; Trubachov, I.N.; Rerberg, M.S.

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