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A hybrid PHB-hydroxyapatite composite for biomedical application: Production, in vitro and in vivo investigation / E. I. Shishatskaya, I. A. Khlusov, T. G. Volova // Journal of Biomaterials Science, Polymer Edition. - 2006. - Vol. 17, Is. 5. - P481-498, DOI 10.1163/156856206776986242 . - ISSN 0920-5063
Кл.слова (ненормированные):
Biocompatibility -- Hydroxyapatite (HA) -- PHB-hydroxyapatite composite -- Polyhydroxyalkanoate (PHA) -- Polyhydroxybutyrate (P(3HB)) -- Properties -- Biocompatibility -- Differential thermal analysis -- Electron microscopy -- Free energy -- Interfacial energy -- Physical properties -- Surface properties -- X ray analysis -- Biomedical application -- Physicochemical properties -- Polyhydroxyalkanoate (PHA) -- Polyhydroxybutyrate (PHB) -- Hydroxyapatite -- hydroxyapatite -- poly(3 hydroxybutyric acid) -- polymer -- biomaterial -- hydroxybutyric acid -- adhesion -- animal cell -- animal tissue -- article -- biomedicine -- bone marrow cell -- cell differentiation -- cell growth -- chemical structure -- composite material -- controlled study -- crystallization -- decomposition -- electron microscopy -- in vitro study -- in vivo study -- melting point -- mouse -- nonhuman -- ossification -- osteoblast -- physical chemistry -- priority journal -- rat -- strength -- structure analysis -- surface property -- synthesis -- temperature measurement -- thermal analysis -- tissue engineering -- wettability -- animal -- biomechanics -- bioremediation -- bone prosthesis -- cattle -- cell culture -- chemistry -- cytology -- differential scanning calorimetry -- drug effect -- human -- materials testing -- prostheses and orthoses -- scanning electron microscopy -- standard -- Wistar rat -- Murinae -- Animals -- Biocompatible Materials -- Biodegradation, Environmental -- Biomechanics -- Bone Substitutes -- Cattle -- Cells, Cultured -- Differential Thermal Analysis -- Durapatite -- Humans -- Hydroxybutyrates -- Materials Testing -- Microscopy, Electron, Scanning -- Osteoblasts -- Prostheses and Implants -- Rats -- Rats, Wistar -- Surface Properties
Аннотация: Samples of a hybrid composite of polyhydroxybutyrate (PHB), a biodegradable polyester, and hydroxyapatite (HA), with different PHB/HA ratios, have been prepared using mechanical-physical method. Electron microscopy, X-ray structure analysis and differential thermal analysis have been used to investigate the structure and physicochemical properties of the composite, depending on the PHB/HA ratio. The properties of the surface of the HA-loaded composite are significantly different from those of the pure polymer. As the HA percentage in the composite increases, free interface energy, the cohesive force, i.e., the strength of the adhesive bond between the composite surface and the water phase, and surface wettability increase. The HA percentage of the composite does not influence its melting temperature, but affects the temperature for the onset of decomposition: as the HA content increases from 0 to 10% (w/w), Td decreases from 260В°C to 225В°C. The degree of crystallinity of PHB/HA increases from 77% to 89% with an increase in the HA fraction from 10% to 50%. Functional properties of the composites have been investigated in vitro and in vivo. The best parameters of growth and differentiation of murine marrow osteoblasts are registered on PHB/HA samples containing 10% and 20% HA. In ectopic bone formation assay it has been proven that the hybrid PHB/HA composites can function as scaffolds and that bone tissue develops on their surface and in pores. В© VSP 2006.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 60036, Russian Federation
Tomsk State University, Tomsk 634021, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Khlusov, I.A.; Volova, T.G.

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Degradation of P(3HB) and P(3HB-co-3HV) in biological media / E. I. Shishatskaya [et al.] // Journal of Biomaterials Science, Polymer Edition. - 2005. - Vol. 16, Is. 5. - P643-657, DOI 10.1163/1568562053783678 . - ISSN 0920-5063
Кл.слова (ненормированные):
Degradation rate -- Fiber properties -- Morphology -- Poly(hydroxybutyrate-co-hydroxyvalerate) (P(3HB-co-3HV)) -- Polyhydroxyalkanoates(PHAs) -- Polyhydroxybutyrate (P(3HB)) -- Copolymers -- Degradation -- Differential scanning calorimetry -- Enzymes -- Morphology -- Scanning electron microscopy -- Tensile strength -- Tissue -- Transmission electron microscopy -- Degradation rate -- Fiber properties -- Polyhydroxyalkanoates (PHAs) -- Polyhydroxybutyrate (P(3HB)) -- Biopolymers -- buffer -- copolymer -- poly(3 hydroxybutyric acid) -- polyhydroxybutyrate hydroxyvalerate copolymer -- unclassified drug -- animal experiment -- animal model -- animal tissue -- article -- biodegradation -- controlled study -- crystal structure -- fiber -- giant cell -- in vitro study -- in vivo study -- macrophage -- morphology -- nonhuman -- pH -- priority journal -- rat -- structure analysis -- tensile strength -- tissue water -- weight reduction -- Animals -- Biodegradation, Environmental -- Buffers -- Humans -- Hydrogen-Ion Concentration -- Hydroxybutyrates -- Macrophages -- Microscopy, Electron, Scanning -- Microscopy, Electron, Transmission -- Muscle, Skeletal -- Polyesters -- Rats -- Rats, Wistar
Аннотация: The biodegradability of oriented fibers made of polyhydroxybutyrate (P(3HB)) and its co-polymer with ?-hydroxyvalerate (P(3HB-co-3HV)) was investigated in buffer solutions and in biological media in vitro and in vivo. The fibers of both polymer types demonstrated resistance to hydrolytic degradation in buffer solutions at 38В°C and pH from 4.5 to 7.0 (for up to 180 days). It has been found that the biodegradation of the fibers in vitro in blood and serum and in vivo is accompanied by weight losses and minor changes in the microstructure with no significant losses in the tensile strength over a long time (up to 180 days). The biodegradation rate of the less crystalline co-polymer P(3HB-co-3HV) fibers was 1.4-2.0-times higher than that of the homopolymer P(3HB). It has also been shown that the degradation of the fibers in vivo is influenced both by tissue fluid enzymes and cells (macrophages and foreign-body giant cells). The fibers were eroded on the surface only with no gross defects and no dramatic effects on their mechanical performance. В© VSP 2005.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 600326, Russian Federation
Department of Cardiac Surgery, University of Glasgow, Royal Infirmary, 10 Alexandra Parade, Glasgow G3, United Kingdom : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Gordeev, S.A.; Puzyr, A.P.

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Degradation of bioplastic in environment / T. G. Volova [и др.] // Doklady Akademii Nauk. - 2004. - Vol. 397, Is. 5. - С. 708-710 . - ISSN 0869-5652
Кл.слова (ненормированные):
Bacteria -- Ecology -- Environmental testing -- Plastics -- Weathering -- Time dependence -- Biomaterials
Аннотация: For the first time degradation kinetics is investigated for polyhydroxyalkanoates (PHA) in the native fresh pond. Its substantial variability in dependence on ecological conditions is shown, new bacteria species (clones) capable for PHA utilization in fresh-water ecosystem are identified.

Scopus
Держатели документа:
Inst. Biofiziki SO RAN, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Golyshev, M.I.; Trusova, M.Yu.; Zhila, N.O.; Kartushinskaya, M.V.

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Production of purified polyhydroxyalkanoates (PHAs) for applications in contact with blood / V. I. Sevastianov [et al.] // Journal of Biomaterials Science, Polymer Edition. - 2003. - Vol. 14, Is. 10. - P1029-1042, DOI 10.1163/156856203769231547 . - ISSN 0920-5063
Кл.слова (ненормированные):
?-hydroxy acids -- Endotoxins -- Hemocompatibility -- Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) -- Polyhydroxyalkanoates (PHAs) -- Polyhydroxybutyrate (PHB) -- bacterium lipopolysaccharide -- carbon -- complement -- copolymer -- hydroxyacid -- long chain fatty acid -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- valeric acid derivative -- adult -- article -- biofilm -- biotechnology -- blood analysis -- blood clotting -- blood compatibility -- cell function -- chemical analysis -- chemical composition -- complement activation -- concentration (parameters) -- controlled study -- gas chromatography -- hemostasis -- human -- human cell -- mass spectrometry -- micromorphology -- nonhuman -- priority journal -- purification -- quantitative analysis -- sampling -- synthesis -- thrombocyte adhesion -- Wautersia eutropha -- Biocompatible Materials -- Blood -- Blood Coagulation Tests -- Chromatography, Gas -- Complement Activation -- Cupriavidus necator -- Fatty Acids -- Humans -- Platelet Adhesiveness -- Polyesters -- Surface Properties
Аннотация: Samples of olyhydroxyalkanoates (PHAs), polyhydroxybutyrate (PHB) and copolymers poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) with 4 and 18 mol% hydroxyvalerate, synthesized by the bacteria Ralstonia eutropha B5786, were investigated. PHA films in contact with blood did not activate the hemostasis system at the level of cell response, but they did activate the coagulation system and the complement reaction. To detect biologically-active components in the PHAs, a detailed analysis of the composition of the polymers was conducted. Gas chromatography-mass spectrometry revealed long-chain fatty acids (FAs) in the tested PHAs. Their total concentration in the polymer ranged from tenths of mol% to 2-3 mol%, depending on the purification method. C16:0 constituted the largest proportion, up to 70%. Of the long-chain hydroxy acids, only ?-OH-C14:0 was detected and it did not exceed 0.06 mol%. The analysis of the hemocompatibility properties of the PHAs purified by a specialized procedure, including the quantitative and morphological estimation of platelets adherent to the surface of polymer films, the plasma recalcification time and complement activation studies, indicated that PHB and PHBV can be used in contact with blood. It has been found out that the lipopolysaccharides of bacteria producing PHAs, which contain mostly long-chain hydroxy acids, can be the factor activating the hemostasis systems. Thus, the technology of PHA purification must satisfy rather stringent specific requirements.

Scopus
Держатели документа:
Inst. of Transplantol. Artif. Organs, Russian Ministry of Health, Shchukinskaya 1, 123182 Moscow, Russian Federation
Inst. of Biophys. of Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sevastianov, V.I.; Perova, N.V.; Shishatskaya, E.I.; Kalacheva, G.S.; Volova, T.G.

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Tissue response to biodegradable suture threads made of polyoxyalkanoates / E. I. Shishatskaya [и др.] // Meditsinskaya Tekhnika. - 2002. - Is. 4. - С. 23-29 . - ISSN 0025-8075
Кл.слова (ненормированные):
Biocompatibility -- Biodegradation -- Implants (surgical) -- Materials testing -- Organic polymers -- Polyoxyalkanoates -- Suture threads -- Biomaterials -- biomaterial -- polymer -- animal -- article -- bioremediation -- female -- metabolism -- rat -- suture -- Wistar rat -- Animals -- Biocompatible Materials -- Biodegradation, Environmental -- Female -- Polymers -- Rats -- Rats, Wistar -- Sutures
Аннотация: The paper presents data on the materials and methods of obtaining suture threads based on of the polymer batty acid derivatives (polyoxyalkanoates). Results of the study of tissue response to biodegradable polyoxyalkanoate suture threads in the postoperative period are analyzed. These suture threads have been shown to have the required strength and meet a number of other requirements for currently available materials.

Scopus
Держатели документа:
Inst. Biofiziki SO RAN, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Efremova, S.N.; Puzyr, S.P.; Mogil'naya, O.A.

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Study of the biological properties of polyoxyalkanoates in the in vivo chronic experiment / E. I. Shishatskaya, T. G. Volova, T. G. Popova // Meditsinskaya Tekhnika. - 2002. - Is. 4. - С. 29-33 . - ISSN 0025-8075
Кл.слова (ненормированные):
biomaterial -- polymer -- animal -- article -- bioremediation -- comparative study -- female -- metabolism -- rat -- suture -- Wistar rat -- Animals -- Biocompatible Materials -- Biodegradation, Environmental -- Female -- Polymers -- Rats -- Rats, Wistar -- Sutures -- Biocompatibility -- Immunology -- Implants (surgical) -- Materials testing -- Organic polymers -- Polyoxyalkanoates -- Rat models -- Suture threads -- Biomaterials
Аннотация: Chronic experiments on mature Wistar rats studied the biological properties of suture threads from two types of polyoxyalkanoates (POA), such as polyoxybutyrate (POB) and the copolymer of POB and polyoxyvalerate (POV) (POB/POV) as compared to the conventional surgical material (silk and catgut). The physiological and biochemical parameters that reflect the status of the animals, including their behavior, height and development, the weight of the body and viscera, the morphology of peripheral blood, the biochemical parameters of blood, the activity of some enzymes, as well as histological findings of the viscera, and lymphoid tissue responses were assessed. POA was found to produce no negative effect on the physiological, biochemical, and functional characteristics of the animals irrespective of the duration of contact with the internal medium of the body and of the chemical composition of a material.

Scopus
Держатели документа:
Inst. Biofiziki SO RAN, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Popova, T.G.

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Biocompatibility and resorption of intravenously administered polymer microparticles in tissues of internal organs of laboratory animals / E. Shishatskaya [et al.] // Journal of Biomaterials Science, Polymer Edition. - 2011. - Vol. 22, Is. 16. - P2185-2203, DOI 10.1163/092050610X537138 . - ISSN 0920-5063
Кл.слова (ненормированные):
14C products of polymer resorption -- intravenous administration -- microparticles -- Resorbable polyhydroxybutyrate -- tissues of internal organs -- 3-hydroxybutyric acid -- sup14/supC products of polymer resorption -- Adverse effect -- Drug-delivery systems -- Growth and development -- High molecular weight -- Initial molecular weight -- Initial values -- Internal organs -- Intravenous administration -- Laboratory animals -- Local response -- matrix -- Mean diameter -- Micro-particles -- Polymer degradation -- Polymer microparticles -- Preparation process -- Radioactivity level -- Residual content -- Resorbable -- Sustained-release -- Tail veins -- Wistar rat -- Animals -- Biocompatibility -- Degradation -- Histology -- Molecular weight -- Morphology -- Radiation -- Radioactivity -- Rats -- Tissue -- poly(3 hydroxybutyric acid) -- animal experiment -- animal tissue -- article -- biocompatibility -- concentration process -- controlled study -- degradation -- drug delivery system -- female -- gel permeation chromatography -- heart -- kidney parenchyma -- liver -- lung parenchyma -- molecular weight -- nonhuman -- organ culture -- priority journal -- radioactivity -- rat -- spleen -- Animals -- Animals, Laboratory -- Biocompatible Materials -- Drug Delivery Systems -- Female -- Infusions, Intravenous -- Materials Testing -- Microspheres -- Molecular Weight -- Particle Size -- Polymers -- Rats -- Rats, Wistar -- Tissue Distribution -- Animalia -- Rattus -- Rattus norvegicus
Аннотация: Specimens of 14C-labeled polymer of 3-hydroxybutyric acid, P(3-HB), with different initial molecular weights, were used to prepare microparticles, whose morphology was not influenced by the M w of the polymer. During the particle preparation process, P(3-HB) molecular weight decreased by 15-20%. Sterile microparticles (mean diameter 2.4 ?m) were injected into the tail veins of Wistar rats (5 mg/rat). The effects of the particles administered to rats were studied based on the general response of animals and local response of internal organ tissues and blood morphology; no adverse effects on growth and development of the animals or unfavorable changes in the structure of the tissues of internal organs were observed. Measurements of radioactivity in tissues showed that 14C concentrations are different in different organs, changing during the course of the experiment. The main targets for 14C-labeled microparticles were tissues of the liver, spleen, and kidneys. Comparison of radioactivity levels and residual contents of high-molecular-weight matrix in tissues suggested that the most rapid metabolism and degradation of P(3-HB) occurred in the liver and spleen. Gel-permeation chromatography showed that at 3 h after the microparticles were injected into the bloodstream, polymer degradation started in all examined organs, except the lungs; at 12 weeks, the M w of the polymer matrix was as low as 20-30% of its initial value. The presence of high-molecular-weight (undegraded) polymer in the tissues at 12 weeks after administration of the particles suggests that P(3-HB) is degraded in tissues of internal organs slowly and, hence, P(3-HB)-based microparticles can be used as sustained-release drug-delivery systems. В© 2011 VSP.

Scopus
Держатели документа:
Institute of Biophysics, SB RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, Svobodnyi Avenue, Krasnoyarsk 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.; Goreva, A.; Kalacheva, G.; Volova, T.

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Investigation of polyhidroxyalkanoates for tissues substitution / E. Shishatskaya, T. Volova // 24th European Conference on Biomaterials - Annual Conference of the European Society for Biomaterials. - 2011. - 24th European Conference on Biomaterials, EBS 2011 (4 September 2011 through 8 September 2011, Dublin) Conference code: 100603

Scopus
Держатели документа:
Institute of Biophysics RAS SB, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.; Volova, T.

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Electrospinning of polyhydroxyalkanoate fibrous scaffolds: effects on electrospinning parameters on structure and properties [Text] / T. . Volova [et al.] // J. Biomater. Sci.-Polym. Ed. - 2014. - Vol. 25, Is. 4. - P370-393, DOI 10.1080/09205063.2013.862400. - Cited References: 52. - This study was financially supported by Project 'Biotechnologies of novel biomaterials: innovative biopolymers and devices for biomedicine' (Agreement No. 1 of 15.02.2013 to Agreement No. 11.G34.31.0013) in accordance with Resolution No. 220 of the Government of the Russian Federation of April 9, 2010, 'On measures designed to attract leading scientists to the Russian institutions of higher learning' and Grant of the RF President for supporting young Doctors of Sciences No. MD-3112.2012.4. . - ISSN 0920-5063

РУБ Engineering, Biomedical + Materials Science, Biomaterials + Polymer Science
Рубрики:
TISSUE ENGINEERING APPLICATIONS
   FIBER MATS
   POLY 3-HYDROXYBUTYRATE
   POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)
   BIOCOMPATIBILITY
   PROLIFERATION
   FABRICATION
   NANOFIBERS
   COPOLYMERS
   MEMBRANES
Кл.слова (ненормированные):
electrospinning -- polyhydroxyalkanoates -- ultrafine fibers -- physical-mechanical properties -- fibroblast cells
Аннотация: In this study, electrospinning was used to prepare ultrafine fibers from PHAs with different chemical compositions: P(3HB) and copolymers: P(3HB-co-4HB), P(3HB-co-3HV), and P(3HB-co-3HHx). The main process parameters that influence ultrafine fiber diameter and properties (polymer concentration, solution feeding rate, working distance, and applied voltage) have been investigated and their effects evaluated. The study revealed electrospinning parameters for the production of high-quality ultrafine fibers and determined which parameters should be varied to tailor the properties of the products. This study is the first to compare biological and physical-mechanical parameters of PHAs with different chemical compositions as dependent upon the fractions of monomers constituting the polymers and ultrafine fiber orientation. Mechanical strength of aligned ultrafine fibers prepared from different PHAs is higher than that of randomly oriented ones; no significant effect of ultrafine fiber orientation on surface properties has been found. None of the fibrous scaffolds produced by electrospinning from PHAs had any adverse effects on attachment, growth, and viability of NIH 3T3 mouse fibroblast cells, and all of them were found to be suitable for tissue engineering applications.

WOS,
Scopus
Держатели документа:
[Volova, Tatiana
Sukovatyi, Aleksey
Nikolaeva, Elena] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
[Goncharov, Dmitriy
Shishatskaya, Ekaterina] Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Krasnoyarsk 660041, Russia
[Shabanov, Alexander] Russian Acad Sci, LV Kirenskii Inst Phys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН
ИФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T...; Goncharov, D...; Sukovatyi, A...; Shabanov, A...; Nikolaeva, E...; Shishatskaya, E...; Project 'Biotechnologies of novel biomaterials: innovative biopolymers and devices for biomedicine' [1, 11.G34.31.0013]; Government of the Russian Federation [220]; RF President for supporting young Doctors of Sciences [MD-3112.2012.4]

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10.

A Glucose-Utilizing Strain, Cupriavidus euthrophus B-10646: Growth Kinetics, Characterization and Synthesis of Multicomponent PHAs [Text] / T. . Volova [et al.] // PLoS One. - 2014. - Vol. 9, Is. 2. - Ст. e87551, DOI 10.1371/journal.pone.0087551. - Cited References: 64. - This study was financially supported by Project "Biotechnologies of novel biomaterials: Innovative Biopolymers and Biomedicine Devices" (Agreement No. 11.G34.31.0013 with Amendment No. 1 of 15 February 2013) in accordance with Resolution No. 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning." The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. . - ISSN 1932-6203

РУБ Multidisciplinary Sciences
Рубрики:
RALSTONIA-EUTROPHA
   BIODEGRADABLE POLYHYDROXYALKANOATES
   AEROMONAS-HYDROPHILA
   ESCHERICHIA-COLI
   MOLECULAR-WEIGHT
   SURFACE-ENERGY
   NORTH PACIFIC
   TERPOLYESTER
   BIOSYNTHESIS
   POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE-CO-3-HYDROXYHEXANOATE)
Аннотация: This study investigates kinetic and production parameters of a glucose-utilizing bacterial strain, C. eutrophus B-10646, and its ability to synthesize PHA terpolymers. Optimization of a number of parameters of bacterial culture (cell concentration in the inoculum, physiological activity of the inoculum, determined by the initial intracellular polymer content, and glucose concentration in the culture medium during cultivation) provided cell concentrations and PHA yields reaching 110 g/L and 80%, respectively, under two-stage batch culture conditions. Addition of precursor substrates (valerate, hexanoate, propionate, c-butyrolactone) to the culture medium enabled synthesis of PHA terpolymers, P(3HB/3HV/4HB) and P(3HB/3HV/3HHx), with different composition and different molar fractions of 3HB, 3HV, 4HB, and 3HHx. Different types of PHA terpolymers synthesized by C. eutrophus B-10646 were used to prepare films, whose physicochemical and physicalmechanical properties were investigated. The properties of PHA terpolymers were significantly different from those of the P3HB homopolymer: they had much lower degrees of crystallinity and lower melting points and thermal decomposition temperatures, with the difference between these temperatures remaining practically unchanged. Films prepared from all PHA terpolymers had higher mechanical strength and elasticity than P3HB films. In spite of dissimilar surface structures, all films prepared from PHA terpolymers facilitated attachment and proliferation of mouse fibroblast NIH 3T3 cells more effectively than polystyrene and the highly crystalline P3HB.

WOS
Держатели документа:
[Volova, Tatiana
Kiselev, Evgeniy
Nikolaeva, Elena
Sukovatiy, Aleksey
Shishatskaya, Ekaterina] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia
[Volova, Tatiana
Vinogradova, Olga
Shishatskaya, Ekaterina] Siberian Fed Univ, Krasnoyarsk, Russia
[Chistyakov, Anton] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T...; Kiselev, E...; Vinogradova, O...; Nikolaeva, E...; Chistyakov, A...; Sukovatiy, A...; Shishatskaya, E...; Project "Biotechnologies of novel biomaterials: Innovative Biopolymers and Biomedicine Devices" [11.G34.31.0013]

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11.

Production of purified polyhydroxyalkanoates (PHAs) for applications in contact with blood [Text] / V. I. Sevastianov [et al.] // J. Biomater. Sci.-Polym. Ed. - 2003. - Vol. 14, Is. 10. - P. 1029-1042, DOI 10.1163/156856203769231547. - Cited References: 34 . - ISSN 0920-5063

РУБ Engineering, Biomedical + Materials Science, Biomaterials + Polymer Science
Рубрики:
BIODEGRADABLE POLYESTERS
POLYMERS
Кл.слова (ненормированные):
polyhydroxyalkanoates (PHAs) -- polyhydroxybutyrate (PHB) -- poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) -- endotoxins -- beta-hydroxy acids -- hemocompatibility
Аннотация: Samples of olyhydroxyalkanoates (PHAs), polyhydroxybutyrate (PHB) and copolymers poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) with 4 and 18 mol% hydroxyvalerate, synthesized by the bacteria Ralstonia eutropha B5786, were investigated. PHA films in contact with blood did not activate the hemostasis system at the level of cell response, but they did activate the coagulation system and the complement reaction. To detect biologically-active components in the PHAs, a detailed analysis of the composition of the polymers was conducted. Gas chromatography-mass spectrometry revealed long-chain fatty acids (FAs) in the tested PHAs. Their total concentration in the polymer ranged from tenths of mol% to 2-3 mol%, depending on the purification method. C-16:0 constituted the largest proportion, up to 70%. Of the long-chain hydroxy acids, only beta-OH-C-14:0 was detected and it did not exceed 0.06 mol%. The analysis of the hemocompatibility properties of the PHAs purified by a specialized procedure, including the quantitative and morphological estimation of platelets adherent to the surface of polymer films, the plasma recalcification time and complement activation studies, indicated that PHB and PHBV can be used in contact with blood. It has been found out that the lipopolysaccharides of bacteria producing PHAs, which contain mostly long-chain hydroxy acids, can be the factor activating the hemostasis systems. Thus, the technology of PHA purification must satisfy rather stringent specific requirements.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
Russian Minist Hlth, Inst Transplantol Artificial Organs, Moscow 123182, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sevastianov, V.I.; Perova, N.V.; Shishatskaya, E.I.; Kalacheva, G.S.; Volova, T.G.

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12.

Laser processing of polymer constructs from poly(3-hydroxybutyrate) / T. G. Volova [et al.] // J. Biomater. Sci. Polym. Ed. - 2015. - Vol. 26, Is. 16. - P1210-1228, DOI 10.1080/09205063.2015.1082810 . - ISSN 0920-5063
Кл.слова (ненормированные):
biocompatibility -- biopolymers -- laser processing -- poly(3-hydroxybutyrate) -- polymer materials -- Biocompatibility -- Biomechanics -- Biopolymers -- Bone -- Cell culture -- Pulsed lasers -- Scaffolds (biology) -- Stem cells -- 3t3 mouse fibroblasts -- Bone marrow -- Bone regeneration -- Laser process -- Mesenchymal stem cell -- Poly-3-hydroxybutyrate -- Polymer materials -- Pulsed mode -- Laser materials processing
Аннотация: CO2 laser radiation was used to process poly(3-hydroxybutyrate) constructs - films and 3D pressed plates. Laser processing increased the biocompatibility of unperforated films treated with moderate uniform radiation, as estimated by the number and degree of adhesion of NIH 3T3 mouse fibroblast cells. The biocompatibility of perforated films modified in the pulsed mode did not change significantly. At the same time, pulsed laser processing of the 3D plates produced perforated scaffolds with improved mechanical properties and high biocompatibility with bone marrow-derived multipotent, mesenchymal stem cells, which show great promise for bone regeneration. © 2015 Taylor & Francis.

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Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation
School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Avenue, Krasnoyarsk, Russian Federation
School of Engineering Physics and Radio Electronics, Siberian Federal University, 79 Svobodnyi Avenue, Krasnoyarsk, Russian Federation
Special Design and Technological Bureau, Nauka Krasnoyarsk Scientific Centre of Siberian Branch, Russian Academy of Sciences, 53 Mir Avenue, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Volova, T. G.; Tarasevich, A. A.; Golubev, A. I.; Boyandin, A. N.; Shumilova, A. A.; Nikolaeva, E. D.; Shishatskaya, E. I.

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13.

Synthesis of P(3HB-co-3HHx) copolymers containing high molar fraction of 3-hydroxyhexanoate monomer by Cupriavidus eutrophus B10646 / T. G. Volova [et al.] // J. Chem. Technol. Biotechnol. - 2016. - Vol. 91, Is. 2. - P416-425, DOI 10.1002/jctb.4592 . - ISSN 0268-2575
Кл.слова (ненормированные):
Growth kinetics -- Physicochemical and mechanical properties -- Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) -- Wild-type strain Cupriavidus eutrophus B10646 -- Biomaterials -- Biomechanics -- Chemical industry -- Cultivation -- Growth kinetics -- Mechanical properties -- Organic compounds -- Polymers -- Sodium -- 3-Hydroxyhexanoate -- Bacterial strains -- Cultivation conditions -- Kinetic properties -- Physico-chemical and mechanical properties -- Physiological range -- Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) -- Wild-type strain -- Strain
Аннотация: BACKGROUND: P(3HB-co-3HHx) copolymers are very promising biomaterials. The main challenge in the production of these polymers is to simultaneously achieve high cell biomass; high P(3HB-co-3HHx) content; and high molar fraction of 3HHx in P(3HB-co-3HHx). The most common approach to production of these copolymers is the use of recombinant bacterial strains. The purpose of this study was to optimize the process of production of P(3HB-co-3HHx) copolymers containing high molar fractions of 3HHx by using the wild-type strain Cupriavidus eutrophus B10646. RESULTS: Kinetic properties of C. eutrophus B10646 were studied during cultivation of the cells on substrates necessary for P(3HB-co-3HHx) synthesis: glucose, nitrogen, sodium hexanoate, and sodium acrylate. The physiological ranges of their effects were determined experimentally, and C. eutrophus B10646 was grown in culture media with different dosages of these substrates. P(3HB-co-3HHx) copolymers with different molar fractions of 3HHx, including high ones (12 to 68%), were synthesized, and their physicochemical and mechanical properties were investigated. CONCLUSION: For the first time, cultivation conditions of Cupriavidus eutrophus B10646 enabled production of high biomass yields (5-6gL-1) and high content of the polymer (60-75%) that contained high 3HHx molar fraction. By varying the 3HB/3HHx ratio, one can change physicochemical and mechanical properties of P(3HB-co-3HHx) copolymers. © 2014 Society of Chemical Industry © 2016 Society of Chemical Industry.

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Держатели документа:
Institute of Biophysics SB RAS, Akademgorodok 50, Krasnoyarsk, Russian Federation
Siberian Federal University, 79 Svobodny pr, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Volova, T. G.; Syrvacheva, D. A.; Zhila, N. O.; Sukovatiy, A. G.

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14.

Experimental wound dressings of degradable PHA for skin defect repair / E. I. Shishatskaya [et al.] // J. Mater. Sci. Mater. Med. - 2016. - Vol. 27, Is. 11, DOI 10.1007/s10856-016-5776-4 . - ISSN 0957-4530
Кл.слова (ненормированные):
Biomaterials -- Cell culture -- Cells -- Cytology -- Enzyme inhibition -- Fibroblasts -- Membranes -- Natural polymers -- Nonwoven fabrics -- Polymer films -- Proteins -- Stem cells -- Weaving -- Electrospun membranes -- Extracellular matrix protein -- Fibroblast cells -- Hydroxyderivative -- Mesenchymal stem cell -- Non-woven membranes -- Wound dressing materials -- Wound healing process -- Tissue
Аннотация: The present study reports construction of wound dressing materials from degradable natural polymers such as hydroxy derivatives of carboxylic acids (PHAs) and 3-hydroxybutyrate/4-hydroxybutyrate [P(3HB/4HB)] as copolymer. The developed polymer films and electrospun membranes were evaluated for its wound healing properties with Grafts—elastic nonwoven membranes carrying fibroblast cells derived from adipose tissue multipotent mesenchymal stem cells. The efficacy of nonwoven membranes of P(3HB/4HB) carrying the culture of allogenic fibroblasts was assessed against model skin defects in Wistar rats. The morphological, histological and molecular studies revealed the presence of fibroblasts on dressing materials which facilitated wound healing, vascularization and regeneration. Further it was also observed that cells secreted extracellular matrix proteins which formed a layer on the surface of membranes and promoted the migration of epidermal cells from the neighboring tissues surrounding the wound. The wounds under the P(3HB/4HB) membrane carrying cells healed 1.4 times faster than the wounds under the cell-free membrane and 3.5 times faster than the wounds healing under the eschar (control).The complete wound healing process was achieved at Day 14. Thus the study highlights the importance of nonwoven membranes developed from degradable P(3HB/4HB) polymers in reducing inflammation, enhancing angiogenic properties of skin and facilitating better wound healing process. © 2016, Springer Science+Business Media New York.

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Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, 50-50 Akademgorodok, Krasnoyarsk, Russian Federation
Siberian Federal University, 79 Svobodniy Ave., Krasnoyarsk, Russian Federation

Доп.точки доступа:
Shishatskaya, E. I.; Nikolaeva, E. D.; Vinogradova, O. N.; Volova, T. G.

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15.

Detection of nanodiamonds in biological samples by EPR spectrometry / E. V. Inzhevatkin [et al.] // Doklad. Biochem. Biophys. - 2017. - Vol. 477, Is. 1. - P394-397, DOI 10.1134/S1607672917060138 . - ISSN 1607-6729
Аннотация: In model experiments in vitro, the applicability of the EPR spectrometry method for the detection of modified nanodiamonds (MNDs) in blood and homogenates of mouse organs has been established. A characteristic signal (g = 2.003, ?H ? 10 G) is observed in the samples of biomaterials containing MNDs, the intensity of which increases linearly with the concentration of nanoparticles in the range of 1.6–200 ?g MNDs per 1 mL of the sample. The EPR method in biomaterials reveals the presence of intrinsic paramagnetic centers, signals from which are superimposed on the signal from the MNDs. However, the intensity of these signals is small, which makes it possible to register the MNDs using EPR spectrometry with the necessary accuracy. The data obtained open up the prospects of using the EPR method for studies of the interorgan distribution, accumulation, and elimination of MNDs during their intravenous injection into experimental animals. © 2017, Pleiades Publishing, Ltd.

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Держатели документа:
International Scientific Center for Studies of Extreme States of Organism, Federal Research Center “Krasnoyarsk Scientific Center,” Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Institute of Biophysics, Federal Research Center “Krasnoyarsk Scientific Center”, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Chemistry and Chemical Technology, Federal Research Center “Krasnoyarsk Scientific Center,” Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Inzhevatkin, E. V.; Baron, A. V.; Maksimov, N. G.; Volkova, M. B.; Puzyr, A. P.; Bondar, V. S.

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16.

Biotechnological wound dressings based on bacterial cellulose and degradable copolymer P(3HB/4HB) / T. G. Volova [et al.] // Int. J. Biol. Macromol. - 2019. - Vol. 131. - P230-240, DOI 10.1016/j.ijbiomac.2019.03.068. - Cited By :1 . - ISSN 0141-8130
Кл.слова (ненормированные):
Bacterial cellulose -- Composites -- Polyhydroxyalkanoates -- 3 hydroxybutyric acid 4 hydroxybutyric acid copolymer -- actovegin -- bacterial cellulose -- cellulose -- collagen type 1 -- copolymer -- cytokeratin 10 -- cytokeratin 14 -- solcoseryl -- unclassified drug -- angiogenesis -- animal cell -- animal experiment -- animal model -- animal tissue -- biochemical analysis -- biocompatibility -- burn -- cell growth -- controlled study -- cutaneous parameters -- differential scanning calorimetry -- drug release -- elemental analysis -- female -- fibroblast -- histopathology -- in vitro study -- in vivo study -- inflammation -- mechanics -- nonhuman -- rat -- Review -- wound healing -- wound planimetry -- X ray analysis
Аннотация: Hybrid wound dressings have been constructed using two biomaterials: bacterial cellulose (BC) and copolymer of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)] – a biodegradable polymer of microbial origin. Some of the experimental membranes were loaded with drugs promoting wound healing and epidermal cells differentiated from multipotent adipose-derived mesenchymal stem cells. A study has been carried out to investigate the structure and physical/mechanical properties of the membranes. The in vitro study showed that the most effective scaffolds for growing fibroblasts were composite BC/P(3HB/4HB) films loaded with actovegin. Two types of the experimental biotechnological wound dressings – BC/P(3HB/4HB)/actovegin and BC/P(3HB/4HB)/fibroblasts – were tested in vivo, on laboratory animals with model third-degree skin burns. Wound planimetry, histological examination, and biochemical and molecular methods of detecting factors of angiogenesis, inflammation, type I collagen, and keratin 10 and 14 were used to monitor wound healing. Experimental wound dressings promoted healing more effectively than VoskoPran – a commercial wound dressing. © 2019

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Держатели документа:
Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, 1 Partizan Zheleznyak Street, Krasnoyarsk, 660022, Russian Federation

Доп.точки доступа:
Volova, T. G.; Shumilova, A. A.; Nikolaeva, E. D.; Kirichenko, A. K.; Shishatskaya, E. I.

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17.

Biodistribution of nanodiamonds in the body of mice using EPR spectrometry / E. Inzhevatkin [et al.] // IET Sci. Meas. Technol. - 2019. - Vol. 13, Is. 7. - P984-988, DOI 10.1049/iet-smt.2018.5594. - Cited References:32. - This work was supported by the Russian Foundation for Basic Research (project no. 16-04-00999). . - ISSN 1751-8822. - ISSN 1751-8830

РУБ Engineering, Electrical & Electronic
Рубрики:
DRUG-DELIVERY
   DETONATION NANODIAMONDS
   NANOMATERIALS
   DOXORUBICIN
Кл.слова (ненормированные):
blood -- biomedical materials -- kidney -- lung -- detonation -- diamond -- nanomedicine -- liver -- muscle -- cellular biophysics -- nanoparticles -- EPR -- imaging -- mice -- EPR spectrometry -- detonation NDs -- electron paramagnetic -- resonance spectrometry -- characteristic EPR signal -- initially injected -- NDs -- detonation -- femoral muscles -- blood -- spleen -- brain -- kidneys -- heart -- lungs -- liver -- biomaterials -- nanodiamonds -- organ homogenates -- nanoparticle concentrations -- inter-organ distribution -- time 2 -- 5 hour -- C
Аннотация: In vitro experiments proved the usefulness of electron paramagnetic resonance (EPR) spectrometry for detecting detonation nanodiamonds (NDs) in samples of biomaterials (blood and homogenates of organs of mice). A characteristic EPR signal (g = 2.003, Delta H similar or equal to 10 G) was detected in biomaterials containing NDs, and its intensity linearly increased at nanoparticle concentrations of between 1.6 and 200 mu g/ml. In vivo experiments demonstrated that EPR spectrometry was effective for monitoring the inter-organ distribution of NDs intravenously injected to mice. In 2.5 h after the injection of NDs, the nanoparticles mainly accumulated in the lungs and liver of the animals - about 25 and 20%, respectively, of the initially injected NDs. The amounts of NDs accumulated in the heart and kidneys were considerably lower. Also, EPR spectrometry did not detect NDs in the blood, spleen, brain, and femoral muscles of mice. Ten days after injection, EPR spectrometry detected redistribution of NDs in mice. The amounts of nanoparticles decreased approximately by a factor of 3.5 in the lungs and increased almost by a factor of 3 in the liver; NDs were detected in the spleen. This study suggests ways to use EPR spectrometry to study the distribution, accumulation, and elimination of detonation NDs injected into laboratory animals.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Fed Res Ctr,Krasnoyarsk Sci Ctr, Krasnoyarsk, Russia.
RAS, SB, Int Sci Ctr Studies Extreme States Organism, Fed Res Ctr,Krasnoyarsk Sci Ctr, Krasnoyarsk, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.
Russian Acad Sci, Siberian Branch, Inst Chem & Chem Technol, Fed Res Ctr,Krasnoyarsk Sci Ctr, Krasnoyarsk, Russia.

Доп.точки доступа:
Inzhevatkin, Evgeny; Baron, Alexey; Maksimov, Nikolai; Volkova, Marina; Puzyr, Alexey; Ronzhin, Nikita; Bondar, Vladimir; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [16-04-00999]

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18.

Polysaccharide-coated iron oxide nanoparticles: Synthesis, properties, surface modification / S. V. Stolyar, V. V. Krasitskaya, L. A. Frank [et al.] // Mater Lett. - 2021. - Vol. 284. - Ст. 128920, DOI 10.1016/j.matlet.2020.128920 . - ISSN 0167-577X
Кл.слова (ненормированные):
Biomaterials -- Magnetic materials -- Nanoparticles -- Polysaccharide -- Iron oxides -- Magnetite -- Magnetite nanoparticles -- Molecules -- Polysaccharides -- Synthesis (chemical) -- Arabinogalactan -- Bioanalytical systems -- Covalent immobilization -- Iron oxide nanoparticle -- Magnetic nanoparticles
Аннотация: In this work, magnetite nanoparticles coated with polysaccharides were synthesized. Arabinogalactan and chitosan were used as polysaccharides. The possibilities of immobilization of biospecific molecules on the surface of the obtained composites were studied. Experiments on covalent immobilization of biospecific molecules on magnetic nanoparticles coated with a polysaccharide showed a high density of immobilized molecules. This suggests the use of such materials in bioanalytical systems or as affinity sorbents. © 2020 Elsevier B.V.

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Держатели документа:
Krasnoyarsk Scientific Center, Federal Research Center KSC SB RAS, Krasnoyarsk, Russian Federation
Kirensky Institute of Physics, Federal Research Center KSC SB RAS, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Biophysics, Federal Research Center KSC SB RAS, Krasnoyarsk, Russian Federation
Astrakhan State University, Astrakhan, Russian Federation
University of Al-qasim Green, College of Biotechnology, Iraq

Доп.точки доступа:
Stolyar, S. V.; Krasitskaya, V. V.; Frank, L. A.; Yaroslavtsev, R. N.; Chekanova, L. A.; Gerasimova, Y. V.; Volochaev, M. N.; Bairmani, M. S.; Velikanov, D. A.

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19.

Polysaccharide-coated iron oxide nanoparticles: Synthesis, properties, surface modification / S. V. Stolyar, V. V. Krasitskaya, L. A. Frank [et al.] // Mater. Lett. - 2021. - Vol. 284. - Ст. 128920, DOI 10.1016/j.matlet.2020.128920. - Cited References:12. - The reported study was carried out with the financial support of the Russian Foundation for Fundamental Research, the Government of the Krasnoyarsk Territory, the Krasnoyarsk Territory Fund for Support of Scientific and Technical Activity in the framework of scientific Project No. 18-43-243003. This work was supported by the Council of the President of the Russian Federation for State Support of Young Scientists and Leading Scientific Schools (project no. MK-1263.2020.3). . - ISSN 0167-577X. - ISSN 1873-4979

РУБ Materials Science, Multidisciplinary + Physics, Applied

Кл.слова (ненормированные):
Nanoparticles -- Biomaterials -- Magnetic materials -- Polysaccharide
Аннотация: In this work, magnetite nanoparticles coated with polysaccharides were synthesized. Arabinogalactan and chitosan were used as polysaccharides. The possibilities of immobilization of biospecific molecules on the surface of the obtained composites were studied. Experiments on covalent immobilization of biospecific molecules on magnetic nanoparticles coated with a polysaccharide showed a high density of immobilized molecules. This suggests the use of such materials in bioanalytical systems or as affinity sorbents. (c) 2020 Elsevier B.V. All rights reserved.

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Держатели документа:
RAS, Krasnoyarsk Sci Ctr, Fed Res Ctr, KSC,SB, Krasnoyarsk, Russia.
RAS, Kirensky Inst Phys, Fed Res Ctr, KSC,SB, Krasnoyarsk, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.
RAS, Inst Biophys, Fed Res Ctr, KSC,SB, Krasnoyarsk, Russia.
Astrakhan State Univ, Astrakhan, Russia.
Univ Al Qasim Green, Coll Biotechnol, Al Qasim, Iraq.

Доп.точки доступа:
Stolyar, S. V.; Krasitskaya, V. V.; Frank, L. A.; Yaroslavtsev, R. N.; Chekanova, L. A.; Gerasimova, Y. V.; Volochaev, M. N.; Bairmani, M. Sh.; Velikanov, D. A.; Russian Foundation for Fundamental ResearchRussian Foundation for Basic Research (RFBR); Government of the Krasnoyarsk Territory; Krasnoyarsk Territory Fund [18-43-243003]; Council of the President of the Russian Federation for State Support of Young Scientists and Leading Scientific SchoolsLeading Scientific Schools Program [MK-1263.2020.3]

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