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A hybrid PHB-hydroxyapatite composite for biomedical application: Production, in vitro and in vivo investigation / E. I. Shishatskaya, I. A. Khlusov, T. G. Volova // Journal of Biomaterials Science, Polymer Edition. - 2006. - Vol. 17, Is. 5. - P481-498, DOI 10.1163/156856206776986242 . - ISSN 0920-5063
Кл.слова (ненормированные):
Biocompatibility -- Hydroxyapatite (HA) -- PHB-hydroxyapatite composite -- Polyhydroxyalkanoate (PHA) -- Polyhydroxybutyrate (P(3HB)) -- Properties -- Biocompatibility -- Differential thermal analysis -- Electron microscopy -- Free energy -- Interfacial energy -- Physical properties -- Surface properties -- X ray analysis -- Biomedical application -- Physicochemical properties -- Polyhydroxyalkanoate (PHA) -- Polyhydroxybutyrate (PHB) -- Hydroxyapatite -- hydroxyapatite -- poly(3 hydroxybutyric acid) -- polymer -- biomaterial -- hydroxybutyric acid -- adhesion -- animal cell -- animal tissue -- article -- biomedicine -- bone marrow cell -- cell differentiation -- cell growth -- chemical structure -- composite material -- controlled study -- crystallization -- decomposition -- electron microscopy -- in vitro study -- in vivo study -- melting point -- mouse -- nonhuman -- ossification -- osteoblast -- physical chemistry -- priority journal -- rat -- strength -- structure analysis -- surface property -- synthesis -- temperature measurement -- thermal analysis -- tissue engineering -- wettability -- animal -- biomechanics -- bioremediation -- bone prosthesis -- cattle -- cell culture -- chemistry -- cytology -- differential scanning calorimetry -- drug effect -- human -- materials testing -- prostheses and orthoses -- scanning electron microscopy -- standard -- Wistar rat -- Murinae -- Animals -- Biocompatible Materials -- Biodegradation, Environmental -- Biomechanics -- Bone Substitutes -- Cattle -- Cells, Cultured -- Differential Thermal Analysis -- Durapatite -- Humans -- Hydroxybutyrates -- Materials Testing -- Microscopy, Electron, Scanning -- Osteoblasts -- Prostheses and Implants -- Rats -- Rats, Wistar -- Surface Properties
Аннотация: Samples of a hybrid composite of polyhydroxybutyrate (PHB), a biodegradable polyester, and hydroxyapatite (HA), with different PHB/HA ratios, have been prepared using mechanical-physical method. Electron microscopy, X-ray structure analysis and differential thermal analysis have been used to investigate the structure and physicochemical properties of the composite, depending on the PHB/HA ratio. The properties of the surface of the HA-loaded composite are significantly different from those of the pure polymer. As the HA percentage in the composite increases, free interface energy, the cohesive force, i.e., the strength of the adhesive bond between the composite surface and the water phase, and surface wettability increase. The HA percentage of the composite does not influence its melting temperature, but affects the temperature for the onset of decomposition: as the HA content increases from 0 to 10% (w/w), Td decreases from 260В°C to 225В°C. The degree of crystallinity of PHB/HA increases from 77% to 89% with an increase in the HA fraction from 10% to 50%. Functional properties of the composites have been investigated in vitro and in vivo. The best parameters of growth and differentiation of murine marrow osteoblasts are registered on PHB/HA samples containing 10% and 20% HA. In ectopic bone formation assay it has been proven that the hybrid PHB/HA composites can function as scaffolds and that bone tissue develops on their surface and in pores. В© VSP 2006.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 60036, Russian Federation
Tomsk State University, Tomsk 634021, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Khlusov, I.A.; Volova, T.G.

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Tissue response to biodegradable suture threads made of polyhydroxyalkanoates / E. I. Shishatskaya [et al.] // Biomedical Engineering. - 2002. - Vol. 36, Is. 4. - P210-217, DOI 10.1023/A:1021184119268 . - ISSN 0006-3398
Кл.слова (ненормированные):
acid phosphatase -- alkaline phosphatase -- polyhydroxyalkanoic acid -- animal experiment -- animal tissue -- article -- biocompatibility -- biodegradability -- controlled study -- elasticity -- enzyme activity -- enzyme mechanism -- female -- histochemistry -- incision -- nonhuman -- physical chemistry -- postoperative period -- rat -- rigidity -- suture -- thickness -- tissue reaction -- wound healing -- Animalia

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Krasnoyarsk Terr. Bur. Pathol. Anat., Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Efremov, S.N.; Puzyr', A.P.; Mogil'Naya, O.A.

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Study of biological properties of polyhydroxyalkanoates in a long-term experiment in vivo / E. I. Shishatskaya, T. G. Volova, T. G. Popova // Biomedical Engineering. - 2002. - Vol. 36, Is. 4. - P218-222, DOI 10.1023/A:1021136203338 . - ISSN 0006-3398
Кл.слова (ненормированные):
hemoglobin -- polyhydroxyalkanoic acid -- animal experiment -- animal tissue -- article -- biocompatibility -- biodegradability -- biosynthesis -- blood cell count -- blood sampling -- comparative study -- controlled study -- DNA synthesis -- elasticity -- enzyme activity -- enzyme mechanism -- erythrocyte sedimentation rate -- female -- in vivo study -- liver function -- nonhuman -- organ weight -- rat -- suture -- Animalia

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Institute of Computer Simulation, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Popova, T.G.

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Results of biomedical investigations of PHB and PHB/PHV fibers / T. Volova [et al.] // Biochemical Engineering Journal. - 2003. - Vol. 16, Is. 2. - P125-133, DOI 10.1016/S1369-703X(03)00038-X . - ISSN 1369-703X
Кл.слова (ненормированные):
Biocompatibility -- Fibers -- Implants -- Poly-3-hydroxybutyrate (PHB) -- Poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate (PHB/PHV) -- Polyhydroxyalkanoates (PHAs) -- Tissue reaction -- Bacteriology -- Calcification (biochemistry) -- Copolymers -- Flammability -- Tissue -- Tissue response -- Biomedical engineering -- acid phosphatase -- poly(3 hydroxybutyric acid) -- poly(3 hydroxybutyric acid) co poly(3 hydroxyvaleric acid) -- polyhydroxyalkanoic acid -- suture material -- unclassified drug -- animal experiment -- article -- bacterial metabolism -- biochemistry -- biocompatibility -- biomedicine -- calcification -- catgut -- correlation analysis -- dose time effect relation -- enzyme activity -- fascia -- fiber -- foreign body -- functional assessment -- giant cell -- implantation -- in vivo study -- inflammation -- intermethod comparison -- macrophage -- materials testing -- multinuclear cell -- muscle injury -- necrosis -- nonhuman -- parameter -- phagocytosis -- physiology -- postoperative period -- priority journal -- process monitoring -- ralstonia eutropha b 5786 -- scar formation -- silk -- strength -- suture -- synthesis -- tissue reaction -- Wautersia eutropha -- wound healing -- Animalia -- Ralstonia -- Wautersia eutropha
Аннотация: The paper presents the results of biomedical investigations of sutures made of polyhydroxyalkanoates (PHAs) of two types (poly-3-hydroxybutyrate (PHB) and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate (PHB/PHV)), synthesized by the bacteria Ralstonia eutropha B 5786 in the experiment on test animals in vivo, in comparison with silk and catgut sutures. PHB and PHB/PHV (PHBV) implants produced no adverse effect on physiological, biochemical and functional parameters of the animals during the post-surgery period. The tested PHA sutures featured the necessary strength throughout the healing period of the muscle-fascial cuts. The reaction of tissues to the implantation of PHB and PHB/PHV fibers fitted into the usual scheme characteristic of the wound process and of the reaction to a foreign-body invasion. This reaction and the reaction of tissues to silk had the similar nature and period of inflammation, but it was much less pronounced than the reaction to catgut. The tissue response to the implantation of PHAs consisted in a short-duration (up to 2 weeks) post-traumatic inflammation and the formation of a fibrous capsule less than 200 ?m thick during weeks 4-8, which in 4-6 months was reduced to 40-60 ?m in the course of reverse development. There were no adverse changes, such as suppurative inflammation, necrosis, calcification, and malignization of the cicatrice, at the site of implantation of PHA filaments, unlike in the cases with silk and catgut. In the case of PHA implantation there was a typical prolonged (throughout the post-surgery monitoring period) pronounced macrophagal stage with a large number of macrophages present. The macrophages were of the phagocytic type and multinucleate giant foreign cells, with a high activity of acid phosphomonoesterase that correlated with the activity of the enzyme in blood. Throughout the period of monitoring no differences in the tissue response to the implantation of the polymer filaments of two PHA types were recorded. В© 2003 Elsevier Science B.V. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 60036, Russian Federation
Inst. Transplantology Artif. Organs, Russian Ministry of Health, Shchukinskaya 1, 123182 Moscow, Russian Federation
Terr. Pathological Anatomy Bureau, Partisan Zheleznyak St. 1, Krasnoyarsk 660049, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.; Shishatskaya, E.; Sevastianov, V.; Efremov, S.; Mogilnaya, O.

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Biomedical investigations of biodegradable PHAs / E. I. Shishatskaya // Macromolecular Symposia. - 2008. - Vol. 269, Is. 1. - P65-81, DOI 10.1002/masy.200850909 . - ISSN 1022-1360
Кл.слова (ненормированные):
Biodegradable polymers -- Biomedical investigations -- PHA -- ABS resins -- Biodegradable polymers -- Biopolymers -- Biotechnology -- Bone -- Cell culture -- Endothelial cells -- Fibers -- Functional polymers -- Osteoblasts -- Polymers -- Surgery -- Abdominal surgeries -- Biocompatible -- Biodegradable -- Biomedical -- Biomedical investigations -- Bone defects -- Ectopic bones -- Hepatocytes -- In vitro -- Microparticles -- Oral surgeries -- Osteogenesis -- PHA -- Polyhydroxybutyrate -- Polymer devices -- Ralstonia -- Russian academy of sciences -- Two types -- Polymer films
Аннотация: This work is a review of the results of biomedical studies of polymer devices (films, fibers, microparticles, 30 implants) made from resorbable PHAs synthesized by the bacterium Wautersia (Ralstonia) eutropha 65786, using the technology developed at the Institute of Biophysics of the Siberian Branch of the Russian Academy of Sciences. Two types of PHAs - polyhydroxybutyrate (PHB) and a hydroxybutyrate/hydroxyvalerate copolymer (PHB/PHV) - have been proven to be biocompatible in vitro in cultures of fibroblasts, endothelial cells, hepatocytes, and osteoblasts, and in short- and long-duration experiments on animals. Polymer films and membranes have been found to be usable as scaffolds for functioning cells and monofilament suture fibers - for stitching muscular-fascial wounds and in abdominal surgery. Ectopic bone formation assay and experiments with the model of segmental osteotomy showed that 30 PHB and PHB/HA implants can be used for reparative osteogenesis. The paper reports beneficial results of using polymers to repair bone defects in oral surgery. Copyright В© 2008 WILEY-VCH Verlag GmbH & Co. KGaA.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, 50, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, Svobodnui Av., 69, Krasnoyarsk, 660148, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.

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In vivo study of 2D PHA matrices of different chemical compositions: tissue reactions and biodegradations [Text] / T. G. Volova [et al.] // Mater. Sci. Technol. - 2014. - Vol. 30, Is. 5. - P549-557, DOI 10.1179/1743284713Y.0000000470. - Cited References: 31. - The study was supported by the project initiated by the Government of the Russian Federation (decree no. 220 of 09.04.2010) for governmental support of scientific research conducted under the guidance of leading scientists at Russian institutions of higher learning (agreement no. 11.G34.31.0013) and the Program of Integrated Research of the Presidium of SB RAS (project no. 96). . - ISSN 0267-0836. - ISSN 1743-2847

РУБ Materials Science, Multidisciplinary + Metallurgy & Metallurgical Engineering
Рубрики:
BIOMEDICAL INVESTIGATIONS
   POLYHYDROXYALKANOATES
   VITRO
   BIOCOMPATIBILITY
   DEGRADATION
   SCAFFOLDS
   CONDUITS
   POLYMERS
Кл.слова (ненормированные):
PHA -- Polyhydroxyalkanoates -- Biocompatibility -- Implantation -- Tissue response -- Biodegradation
Аннотация: Matrices based on resorbable polyhydroxyalkanoates (PHAs) of five types {a homopolymer of 3-hydroxybutyric acid, copolymers of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)], 3-hydroxybutyric and 3-hydroxyvaleric acids [P(3HB/3HV)], 3-hydroxybutyric and 3-hydroxyhexanoic acids [P(3HB/3HHx)]} have been constructed and characterised. No significant differences have been found in tissue response to implantation of these PHAs. Non-coarse fibrous capsules that formed around PHA matrices reached their maximum thickness (60-90 mm) 90 days after implantation; by day 180, the average thickness of the capsules had decreased by 1.5- 2.3 times. The number of foreign body giant cells, resorbing PHAs, remained high. In vivo biodegradation behaviour of polymer matrices is related to the chemical composition of the PHA. Matrices prepared from copolymers P(3HB/4HB) and P(3HB/3HHx) exhibited the fastest degradation rates. P3HB/3HV matrices were degraded more slowly, and P3HB matrices were the most durable. In the PHA matrices that were degraded more slowly, giant cell reaction developed later.

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Держатели документа:
[Volova, T. G.
Shishatskaya, E. I.
Nikolaeva, E. D.] Inst Biophys SB RAS, Krasnoyarsk 660036, Russia
[Volova, T. G.
Shishatskaya, E. I.
Nikolaeva, E. D.] Siberian Fed Univ, Inst Modern Biol & Biotechnol, Krasnoyarsk 660041, Russia
[Sinskey, A. J.] MIT, Dept Biol, Cambridge, MA 02139 USA
[Sinskey, A. J.] MIT, Engn Syst Div, Cambridge, MA 02139 USA
[Sinskey, A. J.] MIT, Hlth Sci Technol Div, Cambridge, MA 02139 USA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Shishatskaya, E.I.; Nikolaeva, E.D.; Sinskey, A.J.; Government of the Russian Federation

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Results of biomedical investigations of PHB and PHB/PHV fibers [Text] / T. . Volova [et al.] // Biochem. Eng. J. - 2003. - Vol. 16, Is. 2. - P. 125-133, DOI 10.1016/S1369-703X(03)00038-X. - Cited References: 20 . - ISSN 1369-703X

РУБ Biotechnology & Applied Microbiology + Engineering, Chemical
Рубрики:
POLYHYDROXYALKANOATES
POLYESTERS
POLYMERS
Кл.слова (ненормированные):
polyhydroxyalkanoates (PHAs) -- poly-3-hydroxybutyrate (PHB) -- poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate (PHB/PHV) -- fibers -- implants -- biocompatibility -- tissue reaction
Аннотация: The paper presents the results of biomedical investigations of sutures made of polyhydroxyalkanoates (PHAs) of two types (poly-3-hydroxybutyrate (PHB) and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate (PHB/PHV)), synthesized by the bacteria Ralstonia eutropha B 5786 in the experiment on test animals in vivo, in comparison with silk and catgut sutures. PHB and PHB/PHV (PHBV) implants produced no adverse effect on physiological, biochemical and functional parameters of the animals during the post-surgery period. The tested PHA sutures featured the necessary strength throughout the healing period of the muscle-fascial cuts. The reaction of tissues to the implantation of PHB and PHB/PHV fibers fitted into the usual scheme characteristic of the wound process and of the reaction to a foreign-body invasion. This reaction and the reaction of tissues to silk had the similar nature and period of inflammation, but it was much less pronounced than the reaction to catgut. The tissue response to the implantation of PHAs consisted in a short-duration (up to 2 weeks) post-traumatic inflammation and the formation of a fibrous capsule less than 200 mum thick during weeks 4-8, which in 4-6 months was reduced to 40-60 mum in the course of reverse development. There were no adverse changes, such as suppurative inflammation, necrosis, calcification, and malignization of the cicatrice, at the site of implantation of PHA filaments, unlike in the cases with silk and catgut. In the case of PHA implantation there was a typical prolonged (throughout the post-surgery monitoring period) pronounced macrophagal stage with a large number of macrophages present. The macrophages were of the phagocytic type and multinucleate giant foreign cells, with a high activity of acid phosphomonoesterase that correlated with the activity of the enzyme in blood. Throughout the period of monitoring no differences in the tissue response to the implantation of the polymer filaments of two PHA types were recorded. (C) 2003 Elsevier Science B.V. All rights reserved.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Akademgorodok, Krasnoyarsk 60036, Russia
Russian Minist Hlth, Inst Transplantol Artificial Organs, Moscow 123182, Russia
Territorial Pathol Anat Bur, Krasnoyarsk 660049, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T...; Shishatskaya, E...; Sevastianov, V...; Efremov, S...; Mogilnaya, O...

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The smallest natural high-active luciferase: Cloning and characterization of novel 16.5-kDa luciferase from copepod Metridia longa [Text] / S. V. Markova [et al.] // Biochem. Biophys. Res. Commun. - 2015. - Vol. 457, Is. 1. - P77-82, DOI 10.1016/j.bbrc.2014.12.082. - Cited References:20. - The cloning of cDNA encoding MLuc7 luciferase of M. longa was supported by Bayer AG (Germany); all other studies - by the grant 14-14-01119 of the Russian Science Foundation. We declare that authors have no conflict of interest. . - ISSN 0006-291X. - ISSN 1090-2104

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
CDNA CLONING
   SECRETED LUCIFERASE
   ESCHERICHIA-COLI
   EXPRESSION
Кл.слова (ненормированные):
Bioluminescence -- Coelenterazine -- Copepod luciferase -- Mammalian -- expression -- Real-time imaging
Аннотация: Coelenterazine-dependent copepod luciferases containing natural signal peptide for secretion are a very convenient analytical tool as they enable monitoring of intracellular events with high sensitivity, without destroying cells or tissues. This property is well suited for application in biomedical research and development of cell-based assays for high throughput screening. We report the cloning of cDNA gene encoding a novel secreted non-allelic 16.5-kDa isoform (MLuc7) of Metridia longa luciferase, which, in fact, is the smallest natural luciferase of known for today. Despite the small size, isoform contains 10 conservative Cys residues suggesting the presence of up to 5 S-S bonds. This hampers the efficient production of functionally active recombinant luciferase in bacterial expression systems. With the use of the baculovirus expression system, we produced substantial amounts of the proper folded MLuc7 luciferase with a yield of similar to 3 mg/L of a high purity protein. We demonstrate that MLuc7 produced in insect cells is highly active and extremely thermostable, and is well suited as a secreted reporter when expressed in mammalian cells ensuring higher sensitivity of detection as compared to another Metridia luciferase isoform (MLuc164) which is widely employed in real-time imaging. (C) 2014 Elsevier Inc. All rights reserved.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Photobiol Lab, Siberian Branch, Krasnoyarsk, Russia.
Siberian Fed Univ, Chair Biophys, Krasnoyarsk, Russia.
ИБФ СО РАН

Доп.точки доступа:
Markova, Svetlana V.; Larionova, Marina D.; Burakova, Ludmila P.; Vysotski, Eugene S.; Bayer AG (Germany); Russian Science Foundation [14-14-01119]

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Variation of Spectral Characteristics of Coelenteramide-Containing Fluorescent Protein from Obelia Longissima Exposed to Dimethyl Sulfoxide / A. S. Petrova [et al.] // Russ. Phys. J. - 2016. - Vol. 59, Is. 4. - P562-567, DOI 10.1007/s11182-016-0806-8. - Cited References:33. - This work was supported in part by the Russian Science Foundation (Contract No. 14-14-00076). . - ISSN 1064-8887. - ISSN 1573-9228

РУБ Physics, Multidisciplinary
Рубрики:
CA2+-REGULATED PHOTOPROTEINS
SPECTROSCOPIC PROPERTIES
Кл.слова (ненормированные):
fluorescent coelenteramide-containing fluorescent proteins -- discharged -- obelin -- proton transfer -- dimethyl sulfoxide
Аннотация: Effect of dimethyl sulfoxide (DMSO), a widespread biomedical agent, on spectral-luminescent characteristics of coelenteramide-containing fluorescent protein - discharged obelin - is investigated. Contributions of violet and blue-green spectral components to fluorescence of discharged obelin are elucidated and characterized at different photoexcitation energies. Dependences of these contributions on the DMSO concentration are presented. Spectral changes are related to the destructive effect of DMSO on fluorescent protein and decreasing efficiency of proton transfer to electronically excited states of fluorophore.

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Scopus
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.
Krasnoyarsk State Agrarian Univ, Krasnoyarsk, Russia.

Доп.точки доступа:
Petrova, A. S.; Alieva, R. R.; Belogurova, N. V.; Tirranen, L. S.; Kudryasheva, N. S.; Russian Science Foundation [14-14-00076]

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10.

Porous 3D implants of degradable poly-3-hydroxybutyrate used to enhance regeneration of rat cranial defect / A. A. Shumilova [et al.] // J. Biomed. Mater. Res. Part A. - 2017. - Vol. 105, Is. 2. - P566-577, DOI 10.1002/jbm.a.35933. - Cited References:53. - Contract grant sponsor: The state budget allocated to the fundamental research at the Russian Academy of Sciences; contract grant number: 01201351505 . - ISSN 1549-3296. - ISSN 1552-4965

РУБ Engineering, Biomedical + Materials Science, Biomaterials
Рубрики:
BONE TISSUE REGENERATION
   CRITICAL SIZE DEFECT
   IN-VITRO
   CALVARIAL
Кл.слова (ненормированные):
3D implants -- poly-3-hydroxybutyrate -- MSC differentiation -- grafts for -- osteogenesis -- regeneration of defect cranial
Аннотация: The study describes preparation and testing of porous 3D implants of natural degradable polymer of 3-hydroxybutyric acid P(3HB) for regeneration of bone tissue defects. The ability of the P(3HB) implants to favor attachment and facilitate proliferation and directed differentiation of mesenchymal stem cells (MSCs) was studied in the culture of MSCs isolated from bone marrow and adipose tissue. Tissue-engineered hybrid systems (grafts) constructed using P(3HB) and P(3HB) in combination with osteoblasts were used in experiments on laboratory animals (n=48) with bone defect model. The defect model (5 mm in diameter) was created in the rat parietal bone, and filling of the defect by the new bone tissue was monitored in the groups of animals with P(3HB) implants, with commercial material, and without implants (negative control). Computed tomography (CT) and histologic examination showed that after 120 days, in the group with the osteoblast-seeded P(3HB) implants, the defect was completely closed; in the group with the cell-free P(3HB) implants, the remaining defect was no more than 10% of the initial one (0.5 mm); in both the negative and positive controls, the size of the defect was about 1.0-1.2 mm. These results suggest that P(3HB) has good potential as osteoplastic material for reconstructive osteogenesis. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 566-577, 2017.

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Держатели документа:
Siberian Fed Univ, 79 Svobodnyi Ave, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia.
VF Voino Yasenetsky Krasnoyarsk State Med Univ, 1 Partizan Zheleznyak St, Krasnoyarsk 660022, Russia.

Доп.точки доступа:
Shumilova, A. A.; Myltygashev, M. P.; Kirichenko, A. K.; Nikolaeva, E. D.; Volova, T. G.; Shishatskaya, E. I.; Russian Academy of Sciences [01201351505]

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11.

Variability of fluorescence spectra of coelenteramide-containing proteins as a basis for toxicity monitoring / R. R. Alieva, N. S. Kudryasheva // Talanta. - 2017. - Vol. 170. - P425-431, DOI 10.1016/j.talanta.2017.04.043 . - ISSN 0039-9140
Кл.слова (ненормированные):
Coelenteramide-containing fluorescent protein -- Multicolor fluorescent bioassay -- Obelin -- Primary photochemical process -- Protein destruction -- Proton transfer -- Bioassay -- Biomarkers -- Excited states -- Fluorescence -- Fluorophores -- Ionizing radiation -- Proton transfer -- Toxicity -- Electron-excited state -- Fluorescence spectra -- Fluorescent protein -- Green fluorescent protein -- Obelin -- Photochemical process -- Photochemical properties -- Physicochemical process -- Proteins
Аннотация: Nowadays, physicochemical approach to understanding toxic effects remains underdeveloped. A proper development of such mode would be concerned with simplest bioassay systems. Coelenteramide-Containing Fluorescent Proteins (CLM-CFPs) can serve as proper tools for study primary physicochemical processes in organisms under external exposures. CLM-CFPs are products of bioluminescent reactions of marine coelenterates. As opposed to Green Fluorescent Proteins, the CLM-CFPs are not widely applied in biomedical research, and their potential as colored biomarkers is undervalued now. Coelenteramide, fluorophore of CLM-CFPs, is a photochemically active molecule; it acts as a proton donor in its electron-excited states, generating several forms of different fluorescent state energy and, hence, different fluorescence color, from violet to green. Contributions of the forms to the visible fluorescence depend on the coelenteramide microenvironment in proteins. Hence, CLM-CFPs can serve as fluorescence biomarkers with color differentiation to monitor results of destructive biomolecule exposures. The paper reviews experimental and theoretical studies of spectral-luminescent and photochemical properties of CLM-CFPs, as well as their variation under different exposures – chemicals, temperature, and ionizing radiation. Application of CLM-CFPs as toxicity bioassays of a new type is justified. © 2017

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Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Akademgorodok 50/50, Krasnoyarsk, Russian Federation
Siberian Federal University, Svobodny Prospect 79, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Alieva, R. R.; Kudryasheva, N. S.

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12.

The disulfide-rich Metridia luciferase refolded from E. coli inclusion bodies reveals the properties of a native folded enzyme produced in insect cells / S. V. Markova [et al.] // J. Photochem. Photobiol. B-Biol. - 2017. - Vol. 175. - P51-57, DOI 10.1016/j.jphotobiol.2017.08.024. - Cited References:30. - These studies were funded by RFBR and the Government of Krasnoyarsk Territory according to the research project No. 16-44-242099 and the state budget allocated to the fundamental research at the Russian Academy of Sciences (project No. 0356-2016-0712). . - ISSN 1011-1344

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
GAUSSIA-PRINCEPS LUCIFERASE
   ESCHERICHIA-COLI
   EXPRESSION
   PROTEIN
Кл.слова (ненормированные):
Copepod luciferase -- Disulfide bonds -- Cysteine-rich protein -- Oxidative -- refolding
Аннотация: The bioluminescence of a marine copepod Metridia Tonga is determined by a small secreted coelenterazine-dependent luciferase that uses coelenterazine as a substrate of enzymatic reaction to generate light (lambda(max) = 480 nm). To date, four different isoforms of the luciferase differing in size, sequences, and properties have been cloned by functional screening. All of them contain ten conserved Cys residues that suggests up to five S-S intramolecular bonds per luciferase molecule. Whereas the use of copepod luciferases as bioluminescent reporters in biomedical research in vivo is growing from year to year, their application for in vitro assays is still limited by the difficulty in obtaining significant amounts of luciferase. The most cost-effective host for producing recombinant proteins is Escherichia coli. However, prokaryotic and eukaryotic cells maintain the reductive environment in cytoplasm that hinders the disulfide bond formation and consequently the proper folding of luciferase. Here we report the expression of the MLuc7 isoform of M. longa luciferase in E. colt cells and the efficient procedure for refolding from inclusion bodies yielding a high-active monomeric protein. Furthermore, in a set of identical experiments we demonstrate that bioluminescent and structural features of MLuc7 produced in bacterial cells are identical to those of MLuc7 isoform produced from culture medium of insect cells. Although the yield of high-purity protein is only 6 mg/L, the application of E. coil cells to produce the luciferase is simpler and more cost-effective than the use of insect cells. We expect that the suggested technology of Metridia luciferase production allows obtaining of sufficient amounts of protein both for the development of novel in vitro analytical assays with the use of MLuc7 as a label and for structural studies.

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RAS, Krasnoyarsk Sci Ctr SB, Fed Res Ctr, Photobiol Lab,Inst Biophys SB, Krasnoyarsk, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.

Доп.точки доступа:
Markova, Svetlana V.; Larionova, Marina D.; Gorbunova, Darya A.; Vysotski, Eugene S.; RFBR; Government of Krasnoyarsk Territory [16-44-242099]; Russian Academy of Sciences [0356-2016-0712]

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13.

Bioluminescent and structural features of native folded Gaussia luciferase / M. D. Larionova, S. V. Markova, E. S. Vysotski // J. Photochem. Photobiol. B Biol. - 2018. - Vol. 183. - P309-317, DOI 10.1016/j.jphotobiol.2018.04.050 . - ISSN 1011-1344
Кл.слова (ненормированные):
Bioluminescence -- Coelenterazine -- Copepod luciferase -- Halophilic enzyme -- Kinetic cooperativity
Аннотация: The secreted luciferases responsible for light emission of marine copepods have gained popularity for being used in noninvasive imaging of intracellular events. The secreted luciferase of copepod Gaussia princeps is a one-subunit protein catalyzing coelenterazine oxidation to emit blue light. It consists of the N-terminal variable part that bears a signal peptide for secretion and the C-terminal catalytic domain containing ten highly conserved Cys residues supposing the existence of up to five S–S bonds. Despite wide application of Gaussia luciferase in biomedical research, its biochemical properties are still insufficiently studied due to the general problem of obtaining the proper folded Cys-rich proteins in bacterial cells. Here we report the properties of the proper folded Gaussia luciferase produced in insect cells using baculovirus expression system. This high purity luciferase reveals the highest activity at 15–20 °C but retains only ~20% activity at 37 °C that may hamper its application for in vivo assays. The maximum of bioluminescent activity of GpLuc is found at NaCl concentrations in the range of 1.0–1.5 M and, furthermore, a high NaCl concentration enhances luciferase stability to thermal denaturation, i.e. Gaussia luciferase displays the features characteristic of halophilic enzymes. The studies on bioluminescence kinetics at different coelenterazine concentrations obviously show a positive cooperativity of Gaussia luciferase with coelenterazine (Hill coefficient – 1.8 ± 0.2; K0.5–2.14 ± 0.17 ?M). We suggest this effect to be rather due to the so-called kinetic cooperativity conditioned by conformational changes in response to substrate binding than to the presence of two catalytic sites. © 2018 Elsevier B.V.

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Держатели документа:
Photobiology Laboratory, Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Larionova, M. D.; Markova, S. V.; Vysotski, E. S.

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14.

Bioluminescent SNP genotyping technique: Development and application for detection of melanocortin 1 receptor gene polymorphisms / E. E. Bashmakova [et al.] // Talanta. - 2018. - Vol. 189. - P111-115, DOI 10.1016/j.talanta.2018.06.057 . - ISSN 0039-9140
Кл.слова (ненормированные):
Ca2+-regulated photoprotein obelin -- Genotyping -- Melanocortin 1 receptor gene -- Single nucleotide polymorphisms (SNP) -- Bioluminescence -- Clinical research -- Curricula -- Diagnosis -- Genes -- Oncology -- Biomedical research -- Clinical characteristics -- Development and applications -- Genotyping -- Healthy individuals -- Photoproteins -- Receptor genes -- Single-nucleotide polymorphisms -- Dermatology
Аннотация: SNP genotyping based on the reaction of specific primer extension with the following bioluminescent detection of its products was shown to be potentially applicable for biomedical exploration. The paper describes its elaboration and first application in extensive biomedical research concerning MC1R gene variants’ frequency and associations with clinical characteristics in melanoma patients of Eastern Siberia (Krasnoyarsk region, Russia). Polymorphisms rs 1805007 (R151C), rs 1805008 (R160W), and rs 1805009 (D294H) were detected in 174 DNA samples from patients with histologically proved diagnosis of cutaneous melanoma and in 200 samples from healthy individuals. All the results on bioluminescent SNP genotyping were confirmed by Sanger sequencing. Some features characteristic of the population were found, i.e. melanoma is mostly associated with R160W or R151C while variant D294H is extremely rare; simultaneous carriage of any two investigated variants is also strongly associated with melanoma; R151C is associated with ulceration and consequently the disease course is more aggressive, etc. The design of the technique allows fast evaluation of any known diagnostically important SNP frequencies and associations across population. © 2018 Elsevier B.V.

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Держатели документа:
Siberian Federal University, Svobodny pr. 79, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Akademgorodok 50/50, Krasnoyarsk, Russian Federation
Blokhin Cancer Research Center, Moscow, Russian Academy of Medical Sciences, Kashirskoye Shosse 24, Moscow, Russian Federation
Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk Lavrentiev Avenue 8, Novosibirsk, Russian Federation
State Medical University named after V.F. Voyno-Yasenetsky, Partizana Zheleznyaka St. 1, Krasnoyarsk, Russian Federation
Regional Clinical Oncology Center named after A.I. Kryzhanovsky, 1 Smolenskaya Str.16, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Bashmakova, E. E.; Krasitskaya, V. V.; Bondar, A. A.; Eremina, E. N.; Slepov, E. V.; Zukov, R. A.; Frank, L. A.

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15.

Battle of GLP-1 delivery technologies / M. Yu [et al.] // Adv. Drug Deliv. Rev. - 2018, DOI 10.1016/j.addr.2018.07.009 . - ISSN 0169-409X
Кл.слова (ненормированные):
Albumin fusion -- Exenatide -- Fatty acid conjugate -- Fc fusion -- GLP-1 receptor agonist -- Half-life -- Peptide delivery -- Pharmacokinetics
Аннотация: Glucagon-like peptide-1 receptor agonists (GLP-1 RAs) belong to an important therapeutic class for treatment of type 2 diabetes. Six GLP-1 RAs, each utilizing a unique drug delivery strategy, are now approved by the Food and Drug Administration (FDA) and additional, novel GLP-1 RAs are still under development, making for a crowded marketplace and fierce competition among the manufacturers of these products. As rapid elimination is a major challenge for clinical application of GLP-1 RAs, various half-life extension strategies have been successfully employed including sequential modification, attachment of fatty-acid to peptide, fusion with human serum albumin, fusion with the fragment crystallizable (Fc) region of a monoclonal antibody, sustained drug delivery systems, and PEGylation. In this review, we discuss the scientific rationale of the various half-life extension strategies used for GLP-1 RA development. By analyzing and comparing different approved GLP-1 RAs and those in development, we focus on assessing how half-life extending strategies impact the pharmacokinetics, pharmacodynamics, safety, patient usability and ultimately, the commercial success of GLP-1 RA products. We also anticipate future GLP-1 RA development trends. Since similar drug delivery strategies are also applied for developing other therapeutic peptides, we expect this case study of GLP-1 RAs will provide generalizable concepts for the rational design of therapeutic peptides products with extended duration of action. © 2018 Elsevier B.V.

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Держатели документа:
Department of Pharmaceutical Sciences, College of Pharmacy, University of Michigan, 428 Church St, Ann Arbor, MI, United States
Amneal Pharmaceuticals, 50 Horseblock Rd, Brookhaven, NY, United States
Siberian Federal University, 79 Svobodnuy Ave, Krasnoyarsk, Russian Federation
Institute of Biophysics SBRAS, 50 Akademgorodok, Russian Federation
Biointerfaces Institute, NCRC, 2800 Plymouth Rd, Ann Arbor, MI, United States
Department of Biomedical Engineering, 2200 Bonisteel Blvd, Ann Arbor, MI, United States

Доп.точки доступа:
Yu, M.; Benjamin, M. M.; Srinivasan, S.; Morin, E. E.; Shishatskaya, E. I.; Schwendeman, S. P.; Schwendeman, A.

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16.

Natural-based polymers for biomedical applications / T. G. Volova [et al.] // : Apple Academic Press, 2017. - P1-439, DOI 10.1201/9781315366036
Аннотация: This new book presents the authors’ biomedical studies of natural degradable biopolymers (polyhydroxyalkanoates [PHAs]) and discusses the demand for medical-grade materials and modern trends, focusing on the present status and future potential of PHAs. The authors present and summarize their most important results and findings obtained during the last few years in experimental studies and clinical trials of PHAs at the Institute of Biophysics Siberian Branch of Russian Academy of Science. © 2017 by Apple Academic Press, Inc.

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Держатели документа:
Department of Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation
Laboratory of Chemoautotrophic Biosynthesis, Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Russian Federation
Department of General Surgery, Krasnoyarsk State Medical School, Krasnoyarsk, Russian Federation
Department of Medical Biology, Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Polymer Division, N. M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, Russian Federation
Moscow State Academy of Fine Chemical Technology, Russian Federation
Kazan National Research Technological University, Kazan, Russian Federation

Доп.точки доступа:
Volova, T. G.; Vinnik, Y. S.; Shishatskaya, E. I.; Markelova, N. M.; Zaikov, G. E.

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17.

Genetically encodable bioluminescent system from fungi / A. A. Kotlobay [et al.] // Proc. Natl. Acad. Sci. U. S. A. - 2018. - Vol. 115, Is. 50. - P12728-12732, DOI 10.1073/pnas.1803615115 . - ISSN 0027-8424
Кл.слова (ненормированные):
Bioluminescence -- Fungal luciferase -- Fungal luciferin biosynthesis
Аннотация: Bioluminescence is found across the entire tree of life, conferring a spectacular set of visually oriented functions from attracting mates to scaring off predators. Half a dozen different luciferins, molecules that emit light when enzymatically oxidized, are known. However, just one biochemical pathway for luciferin biosynthesis has been described in full, which is found only in bacteria. Here, we report identification of the fungal luciferase and three other key enzymes that together form the biosynthetic cycle of the fungal luciferin from caffeic acid, a simple and widespread metabolite. Introduction of the identified genes into the genome of the yeast Pichia pastoris along with caffeic acid biosynthesis genes resulted in a strain that is autoluminescent in standard media. We analyzed evolution of the enzymes of the luciferin biosynthesis cycle and found that fungal bioluminescence emerged through a series of events that included two independent gene duplications. The retention of the duplicated enzymes of the luciferin pathway in nonluminescent fungi shows that the gene duplication was followed by functional sequence divergence of enzymes of at least one gene in the biosynthetic pathway and suggests that the evolution of fungal bioluminescence proceeded through several closely related stepping stone nonluminescent biochemical reactions with adaptive roles. The availability of a complete eukaryotic luciferin biosynthesis pathway provides several applications in biomedicine and bioengineering. © 2018 National Academy of Sciences. All Rights Reserved.

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Держатели документа:
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russian Federation
Planta LLC, Moscow, 121205, Russian Federation
Institute of Science and Technology Austria, Klosterneuburg, 3400, Austria
Medical Research Council London Institute of Medical Sciences, Imperial College London, London, W12 0NN, United Kingdom
Centre for Genomic Regulation, Barcelona Institute for Science and Technology, Barcelona, 08003, Spain
Universitat Pompeu Fabra, Barcelona, 08003, Spain
Evrogen JSC, Moscow, 117997, Russian Federation
Institute of Biophysics, Federal Research Center Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Moscow, 142290, Russian Federation
Pirogov Russian National Research Medical University, Moscow, 117997, Russian Federation
Biomedical Nanomaterials, National Research Technological University (MISiS), Moscow, 119049, Russian Federation
Skolkovo Institute of Science and Technology, Moscow, 121205, Russian Federation
Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Sao Paulo, 05508-000, Brazil
Departamento de Oceanografia Fisica, Quimica e Geologica, Instituto Oceanografico, Universidade de Sao Paulo, Sao Paulo, 05508-120, Brazil
Department of Environmental Biology, Chubu University, Kasugai, 487-8501, Japan
Catalan Institution for Research and Advanced Studies (ICREA), Barcelona, 08010, Spain
Departamento de Quimica Fundamental, Instituto de Quimica, Universidade de Sao Paulo, Sao Paulo, 05508-000, Brazil

Доп.точки доступа:
Kotlobay, A. A.; Sarkisyan, K. S.; Mokrushina, Y. A.; Marcet-Houben, M.; Serebrovskaya, E. O.; Markina, N. M.; Somermeyer, L. G.; Gorokhovatsky, A. Y.; Vvedensky, A.; Purtov, K. V.; Petushkov, V. N.; Rodionova, N. S.; Chepurnyh, T. V.; Fakhranurova, L. I.; Guglya, E. B.; Ziganshin, R.; Tsarkova, A. S.; Kaskova, Z. M.; Shender, V.; Abakumov, M.; Abakumova, T. O.; Povolotskaya, I. S.; Eroshkin, F. M.; Zaraisky, A. G.; Mishin, A. S.; Dolgov, S. V.; Mitiouchkina, T. Y.; Kopantzev, E. P.; Waldenmaier, H. E.; Oliveira, A. G.; Oba, Y.; Barsova, E.; Bogdanova, E. A.; Gabaldon, T.; Stevani, C. V.; Lukyanov, S.; Smirnov, I. V.; Gitelson, J. I.; Kondrashov, F. A.; Yampolsky, I. V.

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18.

Biodistribution of nanodiamonds in the body of mice using EPR spectrometry / E. Inzhevatkin [et al.] // IET Sci. Meas. Technol. - 2019. - Vol. 13, Is. 7. - P984-988, DOI 10.1049/iet-smt.2018.5594. - Cited References:32. - This work was supported by the Russian Foundation for Basic Research (project no. 16-04-00999). . - ISSN 1751-8822. - ISSN 1751-8830

РУБ Engineering, Electrical & Electronic
Рубрики:
DRUG-DELIVERY
   DETONATION NANODIAMONDS
   NANOMATERIALS
   DOXORUBICIN
Кл.слова (ненормированные):
blood -- biomedical materials -- kidney -- lung -- detonation -- diamond -- nanomedicine -- liver -- muscle -- cellular biophysics -- nanoparticles -- EPR -- imaging -- mice -- EPR spectrometry -- detonation NDs -- electron paramagnetic -- resonance spectrometry -- characteristic EPR signal -- initially injected -- NDs -- detonation -- femoral muscles -- blood -- spleen -- brain -- kidneys -- heart -- lungs -- liver -- biomaterials -- nanodiamonds -- organ homogenates -- nanoparticle concentrations -- inter-organ distribution -- time 2 -- 5 hour -- C
Аннотация: In vitro experiments proved the usefulness of electron paramagnetic resonance (EPR) spectrometry for detecting detonation nanodiamonds (NDs) in samples of biomaterials (blood and homogenates of organs of mice). A characteristic EPR signal (g = 2.003, Delta H similar or equal to 10 G) was detected in biomaterials containing NDs, and its intensity linearly increased at nanoparticle concentrations of between 1.6 and 200 mu g/ml. In vivo experiments demonstrated that EPR spectrometry was effective for monitoring the inter-organ distribution of NDs intravenously injected to mice. In 2.5 h after the injection of NDs, the nanoparticles mainly accumulated in the lungs and liver of the animals - about 25 and 20%, respectively, of the initially injected NDs. The amounts of NDs accumulated in the heart and kidneys were considerably lower. Also, EPR spectrometry did not detect NDs in the blood, spleen, brain, and femoral muscles of mice. Ten days after injection, EPR spectrometry detected redistribution of NDs in mice. The amounts of nanoparticles decreased approximately by a factor of 3.5 in the lungs and increased almost by a factor of 3 in the liver; NDs were detected in the spleen. This study suggests ways to use EPR spectrometry to study the distribution, accumulation, and elimination of detonation NDs injected into laboratory animals.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Fed Res Ctr,Krasnoyarsk Sci Ctr, Krasnoyarsk, Russia.
RAS, SB, Int Sci Ctr Studies Extreme States Organism, Fed Res Ctr,Krasnoyarsk Sci Ctr, Krasnoyarsk, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.
Russian Acad Sci, Siberian Branch, Inst Chem & Chem Technol, Fed Res Ctr,Krasnoyarsk Sci Ctr, Krasnoyarsk, Russia.

Доп.точки доступа:
Inzhevatkin, Evgeny; Baron, Alexey; Maksimov, Nikolai; Volkova, Marina; Puzyr, Alexey; Ronzhin, Nikita; Bondar, Vladimir; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [16-04-00999]

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19.

Highly-sensitive graphene field effect transistor biosensor using PNA and DNA probes for RNA detection / M. Tian, M. Qiao, C. Shen [et al.] // Appl Surf Sci. - 2020. - Vol. 527. - Ст. 146839, DOI 10.1016/j.apsusc.2020.146839 . - ISSN 0169-4332
Кл.слова (ненормированные):
Graphene field effect transistor -- PNA probe -- RNA detection -- Biosensors -- Clinical research -- Diagnosis -- DNA -- Field effect transistors -- Graphene -- RNA -- Biomedical research -- Clinical diagnosis -- Electrical response -- Graphene field-effect transistors -- Limit of detection -- Quantitative detection -- Three orders of magnitude -- Ultrasensitive detection -- Graphene transistors
Аннотация: DNA probe-based biosensors have been widely developed for detecting a range of analytes. However, the DNA probe-based sensors suffer from many problems, such as long hybridization time, background electrical noise, and relatively poor specificity. In this paper, we report the ultrasensitive detection for RNA by graphene field effect transistor (G-FET) biosensor using PNA and DNA probes. The limit of detection (LOD) of the PNA probe-modified G-FET sensor is down to 0.1 aM, which is three orders of magnitude lower than that of DNA probe-modified G-FET sensor. We demonstrate that both PNA and DNA probe-modified G-FET have great potential in quantitative detection of RNA. A good linear electrical response to RNA concentrations is obtained in a broad range from 0.1 aM to 1 pM for PNA probe-modified G-FET and from 100 aM to 1 pM for DNA probe-modified G-FET, respectively. The PNA probe-modified G-FET sensors significantly reduce the detection time compared to DNA probe-modified G-FET sensors. Moreover, the electrical response of PNA probe-modified G-FET biosensor to non-complementary RNA is negligible, showing high specificity for RNA detection. What's more, the G-FET sensor was also used to detect RNA in human serum, making it a promising way for future detection of RNA in biomedical research and early clinical diagnosis. © 2020 Elsevier B.V.

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Держатели документа:
Shandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, 253023, China
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Tian, M.; Qiao, M.; Shen, C.; Meng, F.; Frank, L. A.; Krasitskaya, V. V.; Wang, T.; Zhang, X.; Song, R.; Li, Y.; Liu, J.; Xu, S.; Wang, J.

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20.

Highly-sensitive graphene field effect transistor biosensor using PNA and DNA probes for RNA detection / M. Tian, M. Qiao, C. C. Shen [et al.] // Appl. Surf. Sci. - 2020. - Vol. 527. - Ст. 146839, DOI 10.1016/j.apsusc.2020.146839. - Cited References:62. - We are grateful for financial support from National Natural Science Foundation of China (11604040, 61671107), Taishan Scholars Program of Shandong Province (tsqn201812104), Natural Science Foundation of Shandong Province (ZR2019PC026) and Qingchuang Science and Technology Plan of Shandong Province (2019KJJ017). . - ISSN 0169-4332. - ISSN 1873-5584

РУБ Chemistry, Physical + Materials Science, Coatings & Films + Physics,
Рубрики:
PEPTIDE NUCLEIC-ACID
LABEL-FREE DETECTION
SELECTIVE RECOGNITION
Кл.слова (ненормированные):
Graphene field effect transistor -- PNA probe -- RNA detection
Аннотация: DNA probe-based biosensors have been widely developed for detecting a range of analytes. However, the DNA probe-based sensors suffer from many problems, such as long hybridization time, background electrical noise, and relatively poor specificity. In this paper, we report the ultrasensitive detection for RNA by graphene field effect transistor (G-FET) biosensor using PNA and DNA probes. The limit of detection (LOD) of the PNA probe modified G-FET sensor is down to 0.1 aM, which is three orders of magnitude lower than that of DNA probe modified G-FET sensor. We demonstrate that both PNA and DNA probe-modified G-FET have great potential in quantitative detection of RNA. A good linear electrical response to RNA concentrations is obtained in a broad range from 0.1 aM to 1 pM for PNA probe-modified G-FET and from 100 aM to 1 pM for DNA probe-modified GFET, respectively. The PNA probe-modified G-FET sensors significantly reduce the detection time compared to DNA probe-modified G-FET sensors. Moreover, the electrical response of PNA probe-modified G-FET biosensor to non-complementary RNA is negligible, showing high specificity for RNA detection. What's more, the G-FET sensor was also used to detect RNA in human serum, making it a promising way for future detection of RNA in biomedical research and early clinical diagnosis.

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Держатели документа:
Dezhou Univ, Inst Biophys, Shandong Key Lab Biophys, Dezhou 253023, Peoples R China.
Krasnoyarsk Sci Ctr SB RAS, Inst Biophys SB RAS, Fed Res Ctr, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Tian, Meng; Qiao, Mei; Shen, Congcong; Meng, Fanlu; Frank, Ludmila A.; Krasitskaya, Vasilisa V.; Wang, Tiejun; Zhang, Xiumei; Song, Ruihong; Li, Yingxian; Liu, Jianjian; Xu, Shicai; Wang, Jihua; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [11604040, 61671107]; Taishan Scholars Program of Shandong Province [tsqn201812104]; Natural Science Foundation of Shandong ProvinceNatural Science Foundation of Shandong Province [ZR2019PC026]; Qingchuang Science and Technology Plan of Shandong Province [2019KJJ017]

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