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Biosynthesis of multi-component polyhydroxyalkanoates by the bacterium Wautersia eutropha / T. G. Volova, G. S. Kalacheva, A. Steinbuchel // Macromolecular Symposia. - 2008. - Vol. 269, Is. 1. - P1-7, DOI 10.1002/masy.200850901 . - ISSN 1022-1360
Кл.слова (ненормированные):
Autotrophic and mixotrophic growth -- Multi-component polyhydroxyalkanoates -- Wautersia eutropha -- Bacteriology -- Biochemical engineering -- Biochemistry -- Biopolymers -- Biotechnology -- Carboxylic acids -- Fatty acids -- Ketones -- Monomers -- Polymers -- Autotrophic and mixotrophic growth -- Carbon chains -- Carbon nutritions -- Even numbers -- Growth conditions -- Hydroxybutyrate -- Hydroxyvalerate -- Mixotrophic -- Multi-component polyhydroxyalkanoates -- Odd numbers -- Physico-chemical properties -- Polyhydroxyalkanoates -- Synthesis of -- Wautersia eutropha -- Wild types -- Acids
Аннотация: The study addresses the effect of different conditions of carbon nutrition on synthesis of polyhydroxyalkanoates by the bacterium Wautersia eutropha. In experiments with two wild type strains (H16 and 65786), it has been first found that under mixotrophic growth conditions - Co2 + co-substrate (alkanoic acids) - bacteria can synthesize multi-component PHAs, consisting of short- and medium-chainlength monomers with carbon chains containing 4 to 8 atoms. It has been shown that PHA composition is determined by the type of the co-substrate. Fatty acids with odd number of carbons induce bacteria to synthesize four- and five-component PHAs with hydroxybutyrate, hydroxyvalerate as major monomers and hydroxyhexanoate, hydroxyheptanoate and hydroxyoctanoate as minor, occasionally occurring, ones. Fatty acids with even number of carbons induce synthesis of not only their respective monomers (hydroxyhexanoate and hydroxyoctanoate) but also hydroxyvalerate, making possible synthesis of four-component PHAs, containing hydroxybutyrate and hydroxyhexanoate as major components (up to 18 mol%). A family of short- and medium-chain-length four- and five-component PHAs were synthesized and their physicochemical properties examined. Copyright В© 2008 WILEY-VCH Verlag GmbH & Co. KGaA.

Scopus
Держатели документа:
Institute of Biophysics, SB, RAS, 660036 Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Molecular Microbiology and Biotechnology, Munster, Germany : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Steinbuchel, A.

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Synthesis of reserve polyhydroxyalkanoates by luminescent bacteria / A. N. Boyandin [et al.] // Microbiology. - 2008. - Vol. 77, Is. 3. - P318-323, DOI 10.1134/S0026261708030119 . - ISSN 0026-2617
Кл.слова (ненормированные):
Biosynthesis -- Chemical structure -- Luminescent bacteria -- Polyhydroxyalkanoates (PHA) -- Bacteria (microorganisms) -- Photobacterium leiognathi -- Vibrio harveyi
Аннотация: The ability of marine luminescent bacteria to synthesize polyesters of hydroxycarboxylic acids (polyhydroxyalkanoates, PHA) as reserve macromolecules was studied. Twenty strains from the collection of the luminescent bacteria CCIBSO (WDCM839) of the Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, assigned to different taxa (Photobacterium leiognathi, Ph. phosphoreum, Vibrio harveyi, and V. fischeri) were analyzed. The most productive strains were identified, and the conditions ensuring high polymer yields in batch culture (40-70% of the cell dry mass weight) were determined. The capacity for synthesizing two-and three-component polymers containing hydroxybutyric acid as the main monomer and hydroxyvaleric and hydroxyhexanoic acids was revealed in Ph. leiognathi and V. harveyi strains. The results allow luminescent microorganisms to be regarded as new producers of multicomponent polyhydroxyalkanoates. В© 2008 MAIK Nauka.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Kalacheva, G.S.; Rodicheva, E.K.; Volova, T.G.

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Biosynthesis of multicomponent polyhydroxyalkanoates by Wautersia eutropha / T. G. Volova [et al.] // Microbiology. - 2007. - Vol. 76, Is. 6. - P704-711, DOI 10.1134/S0026261707060082 . - ISSN 0026-2617
Кл.слова (ненормированные):
Autotrophic and mixotrophic growth -- Multicomponent polyhydroxyalkanoates -- Wautersia eutropha -- Bacteria (microorganisms) -- Cupriavidus necator
Аннотация: The effect of carbon supply on polyhydroxyalkanoate (PHA) synthesis by bacteria Wautersia eutropha was studied. Synthesis of multicomponent PHA composed of short-and long-chain monomers (C4-C8) by two natural strains (H16 and B5786) under mixotrophic conditions (CO2 + alkanoic acids as cosubstrates) was demonstrated for the first time. The PHA composition was shown to be dependent on the cosubstrate type. In the presence of odd fatty acids, four-and five-component polymers were synthesized; hydroxybutyrate, hydroxyvalerate, and hydroxyheptanoate were the major monomers, while hydroxyhexanoate and hydroxyoctanoate were minor. In the presence of even fatty acids, PHA contained not only the corresponding molecules (hydroxyhexanoate and hydroxyoctanoate), but also hydroxyvalerate; synthesis of four-component PHA which contain mainly hydroxybutyrate and hydroxyhexanoate (up to 18 mol %) is therefore possible. A series of four-and five-component PHA was synthesized and their physicochemical characteristics were determined. В© 2007 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Krasnoyarsk State University, Krasnoyarsk, Russian Federation
Institute of Molecular Biology and Biotechnology, Munster, Germany : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Kozhevnikov, I.V.; Steinbuchel, A.

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Kinetic Parameters of a Culture of the Hydrogen-Oxidizing Bacterium Ralstonia eutropha Grown under Conditions Favoring Polyhydroxybutyrate Biosynthesis / T. G. Volova, N. A. Voinov // Applied Biochemistry and Microbiology. - 2003. - Vol. 39, Is. 2. - P166-170, DOI 10.1023/A:1022585912873 . - ISSN 0003-6838
Кл.слова (ненормированные):
hydrogen -- poly(3 hydroxybutyric acid) -- article -- bacterial growth -- bacterium culture -- biosynthesis -- controlled study -- culture medium -- density -- fermentation -- gas -- gravimetry -- heat exchange -- nonhuman -- oxidation -- Ralstonia eutropha -- surface tension -- temperature dependence -- thermogenesis -- Bacteria (microorganisms) -- Ralstonia -- Wautersia eutropha
Аннотация: Kinetic parameters of a culture of the hydrogen-oxidizing bacterium Ralstonia eutropha grown on a gas substrate under conditions favoring autotrophic biosynthesis of polyhydroxybutyrate were studied. The following parameters, making it possible to control and optimize the process in industrial situations, were determined: specific rate of substrate consumption, physical properties of the culture medium, and coefficients of heat emission and mass transfer.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660033, Russian Federation
Siberian State Technol. University, Min. of Educ. of the Russ. Fed., Krasnoyarsk, 660049, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Voinov, N.A.

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Kinetic parameters of a culture of the hydrogen-oxidizing Ralstonia eutropha, grown under the regimen of biosynthesis of polyhydroxybutyrate / T. G. Volova, N. A. Voinov // Prikladnaia biokhimiia i mikrobiologiia. - 2003. - Vol. 39, Is. 2. - С. 189-193 . - ISSN 0555-1099
Кл.слова (ненормированные):
hydrogen -- hydroxybutyric acid -- polyester -- article -- culture medium -- enzyme specificity -- growth, development and aging -- kinetics -- metabolism -- oxidation reduction reaction -- temperature -- Wautersia eutropha -- Culture Media -- Cupriavidus necator -- Hydrogen -- Hydroxybutyrates -- Kinetics -- Oxidation-Reduction -- Polyesters -- Substrate Specificity -- Temperature
Аннотация: Kinetic parameters of a culture of the hydrogen-oxidizing bacterium Ralstonia eutropha, grown on a gas substrate under the conditions favoring autotrophic biosynthesis of polyhydroxybutyrate, were studied. The following parameters, making it possible to control and optimize the process in industrial situations, were determined: specific rate of substrate consumption, physical properties of culture medium, and coefficients of heat emission and mass transfer.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Voinov, N.A.

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Study of biological properties of polyhydroxyalkanoates in a long-term experiment in vivo / E. I. Shishatskaya, T. G. Volova, T. G. Popova // Biomedical Engineering. - 2002. - Vol. 36, Is. 4. - P218-222, DOI 10.1023/A:1021136203338 . - ISSN 0006-3398
Кл.слова (ненормированные):
hemoglobin -- polyhydroxyalkanoic acid -- animal experiment -- animal tissue -- article -- biocompatibility -- biodegradability -- biosynthesis -- blood cell count -- blood sampling -- comparative study -- controlled study -- DNA synthesis -- elasticity -- enzyme activity -- enzyme mechanism -- erythrocyte sedimentation rate -- female -- in vivo study -- liver function -- nonhuman -- organ weight -- rat -- suture -- Animalia

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Institute of Computer Simulation, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Popova, T.G.

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The effect of temperature on the lipid composition of Botryococcus / G. S. Kalacheva [и др.] // Mikrobiologiya. - 2002. - Vol. 71, Is. 3. - С. 336-344 . - ISSN 0026-3656
Кл.слова (ненормированные):
fatty acid -- lipid -- triacylglycerol -- article -- biosynthesis -- chemistry -- comparative study -- green alga -- growth, development and aging -- metabolism -- temperature -- Algae, Green -- Fatty Acids -- Lipids -- Temperature -- Triglycerides
Аннотация: The lipid composition of the green alga Botryococcus was studied at three different cultivation temperatures: suboptimal, optimal, and supraoptimal. Cultivation at the supraoptimal temperature was found to considerably inhibit the synthesis of nearly all intracellular lipids, except for triacylglycerides, and to influence their fatty acid composition. In particular, the content of trienoic fatty acids was significantly lower at the supraoptimal than at the optimal cultivation temperature. At the same time, the fatty acid composition of the extracellular lipids of the alga virtually did not depend on cultivation temperature.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kalacheva, G.S.; Zhila, N.O.; Volova, T.G.; Gladyshev, M.I.

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Cytotoxicity of polyhydroxyalkanoates in animal cell cultures. / E. I. Shishatskaya [et al.] // Doklady Biological Sciences. - 2000. - Vol. 374, Is. 1-6. - P539-542 . - ISSN 0012-4966
Кл.слова (ненормированные):
DNA -- hydroxybutyric acid -- poly(3 hydroxybutyrate) co (3 hydroxyvalerate) -- poly(3 hydroxybutyric acid) -- poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate) -- poly-beta-hydroxybutyrate -- polyester -- RNA -- thymidine -- uridine -- animal -- article -- biosynthesis -- cell division -- cell strain 3T3 -- cell survival -- cytology -- drug effect -- liver -- liver cell -- metabolism -- mouse -- vascular endothelium -- 3T3 Cells -- Animals -- Cell Division -- Cell Survival -- DNA -- Endothelium, Vascular -- Hepatocytes -- Hydroxybutyrates -- Liver -- Mice -- Polyesters -- RNA -- Thymidine -- Uridine

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Eremeev, A.V.; Gitel'zon, I.I.; Setkov, N.A.; Volova, T.G.

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Comparative study of Thermoresistances' cellular mechanisms in representatives of the Gammarus lacustris Sars populations inhabiting Saline Lake Shira (Republic of Khakassia) and a fresh water body in the Cis-Baikal region / Z. M. Shatilina [et al.] // Doklady Biological Sciences. - 2010. - Vol. 434, Is. 1. - P359-362, DOI 10.1134/S0012496610050182 . - ISSN 0012-4966
Кл.слова (ненормированные):
antioxidant -- catalase -- fresh water -- heat shock protein 70 -- reactive oxygen metabolite -- sea water -- Amphipoda -- animal -- article -- biosynthesis -- comparative study -- ecosystem -- heat -- metabolism -- Russian Federation -- Amphipoda -- Animals -- Antioxidants -- Catalase -- Ecosystem -- Fresh Water -- Hot Temperature -- HSP70 Heat-Shock Proteins -- Reactive Oxygen Species -- Seawater -- Siberia -- Gammarus -- Gammarus lacustris

Scopus
Держатели документа:
Institute of Biology, Irkutsk State University, ul. Lenina 3, Irkutsk 664003, Russian Federation
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, pr. Svobodnyi 79, Krasnoyarsk 660041, Russian Federation
Irkutsk State University, ul. Karla Marksa 1, Irkutsk 664003, Russian Federation
Baikal Research Centre, ul. Karla Marksa 5-10, Irkutsk 664003, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shatilina, Z.M.; Gubanov, M.V.; Zadereev, E.S.; Pavlichenko, V.V.; Axenov-Gribanov, D.V.; Sapozhnikova, E.A.; Protopopova, M.V.; Bedulina, D.S.; Timofeyev, M.A.

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10.

Possibility of Salicornia europaea use for the human liquid wastes inclusion into BLSS intrasystem mass exchange / N. A. Tikhomirova [et al.] // Acta Astronautica. - 2008. - Vol. 63, Is. 7-10. - P1106-1110, DOI 10.1016/j.actaastro.2008.01.003 . - ISSN 0094-5765
Кл.слова (ненормированные):
BLSS -- NaCl turnover -- Salicornia europaea -- Space biology -- Acids -- Amides -- Arsenic compounds -- Biochemical engineering -- Biochemistry -- Biomass -- Cellulose -- Curing -- Drying -- Environmental engineering -- Fatty acids -- Garnets -- Health -- Human engineering -- Lipids -- Mineralogy -- Minerals -- Mining -- Nitrates -- Nitrogen -- Plants (botany) -- Polysaccharides -- Polyvinyl alcohols -- Renewable energy resources -- Silica -- Silicate minerals -- Sodium -- Sodium chloride -- Solutions -- Sugar (sucrose) -- Sugars -- Waste utilization -- Biochemical composition -- Biochemical substances -- Bioregenerative life support systems (BLSS) -- Component conditions -- Crude protein (CP) -- Dry weight (DW) -- Essential fatty acids -- Europaea -- H igh concentrations -- Human urine -- Linoleic acid (LA) -- Linolenic -- Lipid content -- Liquid wastes -- Mass exchanges -- Mineral compositions -- Nitrate nitrogen -- Nitrogen nutrition -- Non saturation -- Physico chemical processes -- Plant functions -- Plant lipids -- Reduced nitrogen -- Salicornia europaea -- Soluble sugars -- Wide spectrum -- Nonmetals -- Amides -- Arsenic -- Biochemistry -- Biomass -- Biotechnology -- Cellulose -- Curing -- Drying -- Fatty Acids -- Lipids -- Nonmetals -- Plants -- Polysaccharides -- Sugars
Аннотация: One of the ways of solving the problem of the human liquid wastes utilization in bioregenerative life support systems (BLSS) can be the use of halophytic vegetable plant Salicornia europaea capable of accumulating sodium chloride in rather high concentrations. Since the most specific higher plant function in BLSS, which at present cannot be substituted by physicochemical processes, appears to be the biosynthesis of a wide spectrum of nutritive substances necessary for a human, the object of the given work was the investigation of the S. europaea productivity, biochemical and mineral composition when grown under close to optimal BLSS vegetative component conditions. As the use of human urine after its preliminary physicochemical processing is supposed to be the mineral solution basis for the S. europaea cultivation, it is necessary to clear up the effect of reduced nitrogen on plants growth. Ground research was carried out. Biochemical composition of the S. europaea edible part showed that crude protein was contained in the highest degree. At that the content of crude protein (24% per dry weight) and cellulose (4.7% per dry weight) was higher in the plants grown on solutions containing amide nitrogen in comparison with the plants grown on solutions with nitrate nitrogen (15.4%-3.1% correspondingly). The water-soluble sugar contents were not high in the S. europaea edible part and depending on the nitrogen nutrition form they amounted to 1.1% (amide nitrogen) and 1.5% (nitrate nitrogen). The polysaccharide number (except cellulose) was rather higher and varied from 7.7% to 8.2%. Although the lipid content in the S. europaea plants was relatively low (7% per dry weight), it was shown that the plant lipids are characterized by a high nonsaturation degree mainly due to alpha linolenic and linoleic acids. Nitrogen nutrition form did not significantly affect the S. europaea productivity, and dry edible biomass of one plant was 8.6 g. Sodium and its concentrations predominated in the plant mineral composition and amounted in average to 9% per dry weight. Thus the S. europaea being the vegetable plant it can be the source of several biochemical substances and essential fatty acids. The present work also considers the influence of nitrate and amide forms of nitrogen on S. europaea biochemical and mineral composition. В© 2008 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Akademgorodok, 660036 Krasnoyarsk, Russian Federation
LGCB, Universite B. Pascal, CUST, BP206, 63174 Aubie're, cedex, France : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirova, N.A.; Ushakova, S.A.; Tikhomirov, A.A.; Kalacheva, G.S.; Gros, J.-B.

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11.

Synthesis of biomass and utilization of plants wastes in a physical model of biological life-support system / A. A. Tikhomirov [et al.] // Acta Astronautica. - 2003. - Vol. 53, Is. 4-10. - P249-257, DOI 10.1016/S0094-5765(03)00137-1 . - ISSN 0094-5765
Кл.слова (ненормированные):
Ecosystems -- Microorganisms -- pH -- Photosynthesis -- Plants (botany) -- Synthesis (chemical) -- Waste utilization -- Biological life support systems (BLLS) -- Gas exchange -- Plant respiration -- Biomass -- carbon dioxide -- Agaricales -- article -- biomass -- bioremediation -- growth, development and aging -- hydroponics -- incineration -- metabolism -- methodology -- microbiology -- microclimate -- photosynthesis -- plant physiology -- radish -- space flight -- waste management -- weightlessness -- wheat -- Agaricales -- Biodegradation, Environmental -- Biomass -- Carbon Dioxide -- Ecological Systems, Closed -- Environmental Microbiology -- Hydroponics -- Incineration -- Life Support Systems -- Photosynthesis -- Plant Physiology -- Raphanus -- Space Flight -- Triticum -- Waste Management -- Weightlessness
Аннотация: The paper considers problems of biosynthesis of higher plants' biomass and "biological incineration" of plant wastes in a working physical model of biological LSS. The plant wastes are "biologically incinerated" in a special heterotrophic block involving Califomian worms, mushrooms and straw. The block processes plant wastes (straw, haulms) to produce soil-like substrate (SLS) on which plants (wheat, radish) are grown. Gas exchange in such a system consists of respiratory gas exchange of SLS and photosynthesis and respiration of plants. Specifics of gas exchange dynamics of high plants - SLS complex has been considered. Relationship between such a gas exchange and PAR irradiance and age of plants has been established. Nitrogen and iron were found to the first to limit plants' growth on SLS when process conditions are deranged. The SLS microflora has been found to have different kinds of ammonifying and denitrifying bacteria which is indicative of intensive transformation of nitrogen-containing compounds. The number of physiological groups of microorganisms in SLS was, on the whole, steady. As a result, organic substances - products of exchange of plants and microorganisms were not accumulated in the medium, but mineralized and assimilated by the biocenosis. Experiments showed that the developed model of a man-made ecosystem realized complete utilization of plant wastes and involved them into the intrasystem turnover. В© 2003 International Astronautical Federation. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russian Federation
Universite B. Pascal, Clermont-Ferrand, France
Environ. Contr. Life Support Sect., ESA, Estec Noonvijk, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Ushakova, S.A.; Manukovsky, N.S.; Lisovsky, G.M.; Kudenko, Yu.A.; Koyalev, V.S.; Gribovskaya, I.V.; Tirranen, L.S.; Zolotukhin, I.G.; Gros, J.B.; Lasseur, Ch.

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12.

Recycling efficiencies of C,H,O,N,S, and P elements in a biological life support system based on micro-organisms and higher plants / J. B. Gros [et al.] // Advances in Space Research. - 2003. - Vol. 31, Is. 1. - P195-199, DOI 10.1016/S0273-1177(02)00739-1 . - ISSN 0273-1177
Кл.слова (ненормированные):
Biomass -- Biosynthesis -- Chemical elements -- Earth atmosphere -- Ecosystems -- Life support systems (spacecraft) -- Manned space flight -- Microorganisms -- Plants (botany) -- Wastes -- Compartments -- Space research -- carbon -- carbon dioxide -- hydrogen -- nitrogen -- oxygen -- phosphorus -- sulfur -- space technology -- article -- biological model -- biomass -- chemistry -- comparative study -- computer simulation -- growth, development and aging -- metabolism -- methodology -- microbiology -- microclimate -- plant -- waste management -- Biomass -- Carbon -- Carbon Dioxide -- Computer Simulation -- Ecological Systems, Closed -- Environmental Microbiology -- Hydrogen -- Life Support Systems -- Models, Biological -- Nitrogen -- Oxygen -- Phosphorus -- Plants, Edible -- Sulfur -- Waste Management
Аннотация: MELiSSA is a microorganism based artificial ecosystem conceived as a tool for understanding the behavior of ecosystems and developing the technology for future Manned Space Missions. MELiSSA is composed of four compartments colonized by the microorganisms required by the function of this ecosystem : breakdown of waste produced by men, regeneration of atmosphere and biosynthesis of edible biomass. This paper reports the mass balance description of a Biological Life Support System composed of the MELiSSA loop and of a Higher Plant Compartment working in parallel with the photosynthetic Spirulina compartment producing edible biomass. The recycling efficiencies of the system are determined and compared for various working conditions of the MELiSSA loop with or without the HPC. В© 2002 Published by Elsevier Science Ltd on behalf of COSPAR.

Scopus
Держатели документа:
Lab. de Genie Chimique/Biochimique, Universite B. Pascal, Aubiere cedex 63177, France
ESA/Estec, YVC, P.O. Box 299, Noordwijk, AG 2200, Netherlands
Institute of Biophysics, Siberian branchs RAS, Academgorodok, Krasnoyarsk 630036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gros, J.B.; Poughon, L.; Lasseur, C.; Tikhomirov, A.A.

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13.

Volatile metabolites and external CO2 exchange of wheat cenoses under optimal conditions and thermal stress / I. I. Gitel'son [et al.] // Applied Biochemistry and Microbiology. - 2002. - Vol. 38, Is. 1. - P78-82, DOI 10.1023/A:1013212907872 . - ISSN 0003-6838
Кл.слова (ненормированные):
carbon dioxide -- volatile agent -- article -- biosynthesis -- carbon dioxide transport -- chemical composition -- concentration (parameters) -- controlled study -- cultivar -- metabolite -- nonhuman -- photosynthesis -- photosynthetically active radiation -- plant metabolism -- qualitative analysis -- quantitative analysis -- stress -- temperature sensitivity -- thermal exposure -- thermostability -- wheat -- Rickettsia sp. PAR -- Triticum -- Triticum aestivum
Аннотация: The effects of elevated temperature (35 and 45В°C) on photosynthesis, respiration, and both the qualitative and quantitative compositions of volatile emissions (VE) of wheat (Triticum aestuvum L. cultivar 232) cenoses at light intensities of 70, 150, or 240 W/m2 of photosynthetically available radiation (PAR) were studied. At a PAR of 240 W/m2, the thermal stabilities of photosynthesis and respiration increased at 35В°C and decreased at 45В°C. Elevated temperatures nonuniformly changed the rates and direction of VE syntheses. In this process, the highest increase in VE evolution was observed at 70 W/m2 and 35В°C; the lowest, at 240 W/m2. In addition, the concentrations and composition of VE during the repair period differed from the initial values.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitel'son, I.I.; Tikhomirov, A.A.; Parshina, O.V.; Ushakova, S.A.; Kalacheva, G.S.

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14.

Assessment of plant leaf response to long-term exposure at high temperature using the method of thermoinduced chlorophyll fluorescence. / T. V. Nesterenko [et al.] // Doklady. Biochemistry and biophysics. - 2001. - Vol. 378. - P165-169 . - ISSN 1607-6729
Кл.слова (ненормированные):
chlorophyll -- pigment -- article -- biosynthesis -- chemistry -- light -- metabolism -- plant leaf -- plant physiology -- radiation exposure -- spectrofluorometry -- temperature -- thermodynamics -- time -- Chlorophyll -- Light -- Pigments, Biological -- Plant Leaves -- Plant Physiology -- Spectrometry, Fluorescence -- Temperature -- Thermodynamics -- Time Factors

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Nesterenko, T.V.; Tikhomirov, A.A.; Shikhov, V.N.; Zavorueva, E.N.

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15.

Role of essential fatty acids in trophometabolic interactions in the freshwater ecosystems (a review) / N. N. Sushchik // Zhurnal Obshchei Biologii. - 2008. - Vol. 69, Is. 4. - С. 299-316 . - ISSN 0044-4596
Кл.слова (ненормированные):
essential fatty acid -- fresh water -- animal -- article -- ecosystem -- fish -- food chain -- human -- metabolism -- phytoplankton -- zooplankton -- Animals -- Ecosystem -- Fatty Acids, Essential -- Fishes -- Food Chain -- Fresh Water -- Humans -- Phytoplankton -- Zooplankton -- algae -- Animalia -- Bacteria (microorganisms) -- Invertebrata -- Vertebrata
Аннотация: Polyunsaturated fatty acids (PUFA) of ?3 family are of crucial physiological importance for the most animals and they are an essential and deficient component of human nutrition. These compounds are most effectively synthesized by some groups of algae, hence, aquatic ecosystems are considered to be the main source of these PUFA for human nutrition. Factors controlling the content and distribution of ?3 PUFA in freshwater organisms of basic trophic levels and determined PUFA final production in freshwater ecosystems are considered in the review. PUFA biosynthesis is known to be tightly related to basic fatty acid metabolic pathways. Hence, fatty composition and the PUFA content of major freshwater hydrobiont groups, including bacteria, algae, invertebrates and vertebrates, and environmental and population age effects are described. The peculiarities of PUFA transfer between organisms of various trophic levels are discussed. The essential ?3 PUFA is one of the important parameter of food quality of aquatic consumers and they can determine the rate of energy and matter transfer between producers and primary consumers and, as a result, in a whole freshwater food chain. Analysis of PUFA content and its regulation in biomass of various fish populations indicates that freshwater ecosystems are of the same value in respect of PUFA sources as marine ecosystems. Despite the great practical importance, the studies focused on production and whole pools of ?3 PUFA in different freshwater ecosystems are still scarce and need to be continued.

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Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sushchik, N.N.

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16.

Growth and luminescence of luminous bacteria promoted by agents of microbial origin. / E. K. Rodicheva [et al.] // Journal of bioluminescence and chemiluminescence. - 1993. - Vol. 8, Is. 6. - P293-299 . - ISSN 0884-3996
Кл.слова (ненормированные):
amino acid -- carbohydrate -- folic acid -- luciferase -- nitrogen -- riboflavin -- article -- biosynthesis -- culture medium -- electron microscopy -- growth, development and aging -- kinetics -- luminescence -- metabolism -- Photobacterium -- physiology -- time -- ultrastructure -- Vibrio -- Amino Acids -- Carbohydrates -- Culture Media -- Folic Acid -- Kinetics -- Luciferase -- Luminescence -- Microscopy, Electron -- Nitrogen -- Photobacterium -- Riboflavin -- Time Factors -- Vibrio
Аннотация: The examination of four species of luminous bacteria Photobacterium leiognathi, Photobacterium phosphoreum, Vibrio fischeri and Vibrio harveyi has enabled us to reveal some nutrient medium components effecting growth, luminescence intensity and luciferase synthesis. These agents are nucleic components (nucleotides, nucleotides and amine bases), amino acids and vitamins, which are part of hydrolysates from the biomass of various lithotrophic microorganisms, hydrogen-oxidizing, iron-oxidizing and carboxydobacteria. The effect of promoting agents essentially alters the physiological state and ultrastructure of the cells of luminous bacteria and increases luciferase biosynthesis two- to three-fold compared to a control.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Krasnoyarsk. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Rodicheva, E.K.; Trubachev, I.N.; Medvedeva, S.E.; Egorova, O.I.; , U - Shitova LYu

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17.

A new scheme of lignin biosynthesis and the mechanism of its regulation of functional properties. / D. A. Semenov, N. E. Sudachkova, R. G. Khlebopros // Doklady. Biochemistry and biophysics. - 2002. - Vol. 382. - P50-52 . - ISSN 1607-6729
Кл.слова (ненормированные):
lignin -- peroxidase -- phenol derivative -- polymer -- article -- biosynthesis -- cell wall -- chemistry -- metabolism -- oxidation reduction reaction -- physiology -- plant -- Cell Wall -- Lignin -- Oxidation-Reduction -- Peroxidase -- Phenols -- Plants -- Polymers

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Semenov, D.A.; Sudachkova, N.E.; Khlebopros, R.G.

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18.

Biosynthesis of heteropolymeric polyhydroxyalkanoates by chemolithoautotrophic bacteria / T. G. Volova, G. S. Kalacheva, V. F. Plotnikov // Microbiology. - 1998. - Vol. 67, Is. 4. - P420-424 . - ISSN 0026-2617
Кл.слова (ненормированные):
Carboxydobacteria -- Hydrogen-oxidizing bacteria -- Polyhydroxyalkanoates
Аннотация: Two chemolithoautotrophs - the hydrogen-oxidizing bacterium Alcaligenes eutrophus and the carboxydobacterium Seliberia carboxydohydrogena - were investigated with respect to their ability to produce heteropolymeric polyhydroxyalkanoates (PHA) during mixotrophic growth on CO2 and medium-chain-length (C6 to C9) alkanoic acids. Hydroxy derivatives of the alkanoic acids were incorporated into PHA synthesized by the bacteria studied in proportions dependent on the time of cultivation. Irrespective of the nature of the acid added to the medium, PHAs were found to contain hydroxybutyrate as the main constituent and also hydroxyvalerate and hydroxyhexanoate, whose maximal incorporation was observed 4 to 6 h after the addition of an alkanoic acid to the medium. During further cultivation, the fractions of hydroxyvalerate and hydroxyhexanoate in PHA decreased, and the fraction of hydroxybutyrate and the total intracellular content of the polymer increased. Three-component polyhydroxyalkanoates with different proportions of hydroxybutyrate, hydroxyvalerate, and hydroxyhexanoate were obtained, and some of their properties were analyzed. В© 1998 MAHK Hayka/Interperiodica Publishing.

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Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Plotnikov, V.F.

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19.

Biosynthesis of multi-component polyhydroxyalkanoates by the bacterium Wautersia eutropha [Text] / T. G. Volova, G. S. Kalacheva, A. . Steinbuchel // Macromol. Symp. - 2008. - Vol. 269: 4th European Symposium on Biopolymers (OCT 02-04, 2007, Kusadasi, TURKEY). - P1-7, DOI 10.1002/masy.200850901. - Cited References: 20 . - 7. - ISSN 1022-1360

РУБ Polymer Science
Рубрики:
RALSTONIA-EUTROPHA
   ALCALIGENES-EUTROPHUS
   AEROMONAS-HYDROPHILA
   ENHANCED PRODUCTION
   PHA SYNTHASE
   COPOLYESTERS
   POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYHEXANOATE)
   3-HYDROXYBUTYRATE
   ACID
Кл.слова (ненормированные):
autotrophic and mixotrophic growth -- multi-component polyhydroxyalkanoates -- Wautersia eutropha
Аннотация: The study addresses the effect of different conditions of carbon nutrition on synthesis of polyhydroxyalkanoates by the bacterium Wautersia eutropha. In experiments with two wild type strains (H16 and 85786), it has been first found that under mixotrophic growth conditions -CO2+ co-substrate (alkanoic acids) - bacteria can synthesize multi-component PHAs, consisting of short- and medium-chain-length monomers with carbon chains containing 4 to 8 atoms. It has been shown that PHA composition is determined by the type of the co-substrate. Fatty acids with odd number of carbons induce bacteria to synthesize four- and five-component PHAs with hydroxybutyrate, hydroxyvalerate as major monomers and hydroxyhexanoate, hydroxyheptanoate and hydroxyoctanoate as minor, occasionally occurring, ones. Fatty acids with even number of carbons induce synthesis of not only their respective monomers (hydroxyhexanoate and hydroxyoctanoate) but also hydroxyvalerate, making possible synthesis of four-component PHAs, containing hydroxybutyrate and hydroxyhexanoate as major components (up to 18 mol%). A family of short- and medium-chain-length four- and five-component PHAs were synthesized and their physicochemical properties examined.

Держатели документа:
[Volova, Tatiana G.
Kalacheva, Galina S.] Inst Biophys SB RAS, Krasnoyarsk 660036, Russia
[Volova, Tatiana G.] Siberian Fed Univ, Krasnoyarsk, Russia
[Steinbuechel, Alexander] Inst Mol Microbiol & Biotechnol, Munster, Germany : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Steinbuchel, A...

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20.

Theoretical and experimental decisions in the creation of an artificial ecosystem for human life support in space. / L. V. Kirensky [et al.] // Life sciences and space research. - 1971. - Vol. 9. - P75-80 . - ISSN 0075-9422
Кл.слова (ненормированные):
article -- bacterium -- biotechnology -- Chlorella -- energy metabolism -- human -- instrumentation -- man machine interaction -- metabolism -- microclimate -- space flight -- weightlessness -- Bacteria -- Biotechnology -- Chlorella -- Ecological Systems, Closed -- Energy Metabolism -- Environment, Controlled -- Humans -- Life Support Systems -- Man-Machine Systems -- Space Flight -- Weightlessness
Аннотация: All of man's former space flights were not real ventures into space in the biological sense, as his life was supported with unregenerated earth supplies. The coming stage of space exploration requires man's long existence in the cosmos and on the other planets. This stage of man's activity outside the earth become possible only by creating small man-made ecosystems, permitting the support of his metabolism by the recycling of substances of the terrestrial biosphere. Creation of such systems is a new scientific and technical task. Man-made ecosystems are a new product of man's activity, which have no complete analogy, either in nature, or in technology. Stochastic mechanisms, which stabilize biogeocenosis, cannot be effective in small ecosystems. A technique of parametric control over biosynthesis made it possible to calculate, and put to practice, an ecosystem for man with a cyclic regeneration of the atmosphere, water and, partially, food. The specific bio-technological properties of small man-made ecosystems are being analysed. The possibility of their application for man's excursions into space and for the settlement of other planets is being considered.

Scopus
Держатели документа:
Institute of Physics, Akademgorodok, Krasnoyarsk, USSR. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kirensky, L.V.; Gitelson, I.I.; Terskov, I.A.; Kovrov, B.G.; Lisovsky, G.M.; Okladnikov, Y.N.

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