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Surface properties of nanodiamond films deposited by electrophoresis on Si(100) / E. Maillard-Schaller [et al.] // Diamond and Related Materials. - 1999. - Vol. 8, Is. 2-5. - P805-808 . - ISSN 0925-9635
Кл.слова (ненормированные):
Energy band diagram -- Nanodiamond -- Raman spectroscopy -- Surface characterization -- Band structure -- Electrodeposition -- Electrophoresis -- Hydrogen -- Nanostructured materials -- Nitrogen -- Oxidation -- Oxygen -- Phonons -- Plasma applications -- Silicon wafers -- Surface properties -- Dielectrophoresis -- Negative electron affinity (NEA) -- Phonon confinement effect -- Diamond films
Аннотация: The surface properties of diamond nanoparticles (40-50 A in diameter) have been investigated by X-ray photoelectron spectroscopy (XPS), UV photoelectron spectroscopy (UPS) and Raman spectroscopy. The diamond nanoparticles have been deposited on flat Si(100) substrates by electrophoresis/dielectrophoresis. The as-deposited films are strongly oxidized and present a 1-2% nitrogen content. After treatment at 850 В°C in H2 plasma for 60 min, the oxygen is removed, and the position of the C 1s core-level peak indicates a n-type electronic comportment of the diamond nanoparticles. Raman spectroscopy of the as-deposited film shows a sp3 contribution at 1321 cm-1 and a sp2 contribution around 1620 cm-1. The 12 cm-1 shift of the sp3 contribution with respect to the bulk diamond peak at 1333 cm-1 is attributed to a phonon confinement effect due to the size of the diamond particles. The H2 plasma treatment induces a size decrease of the nanocrystallites confirmed by Raman and scanning electron microscopy (SEM) measurements. UPS spectroscopy shows a negative electron affinity of -0.2 eV of the hydrogenated nanodiamond film.

Scopus
Держатели документа:
Solid State Physics Department, University of Fribourg, 1700, Fribourg, Switzerland
Institute of Christallography, 117333, Moscow, Russian Federation
Institute of Biophysics, 660036, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Maillard-Schaller, E.; Kuettel, O.M.; Diederich, L.; Schlapbach, L.; Zhirnov, V.V.; Belobrov, P.I.

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Kinetic characteristics of the theoretical ecosystems with different extent of openness / Ya. V. Galayda [et al.] // Advances in Space Research. - 2005. - Vol. 35, Is. 9 SPEC. ISS. - P1516-1520, DOI 10.1016/j.asr.2005.01.034 . - ISSN 0273-1177
Кл.слова (ненормированные):
Mathematic modeling -- Net primary production -- Residual concentration of biogenic limiting substances -- Trophic chain length -- Mathematic modeling -- Net primary production -- Residual concentration of biogenic limiting substances -- Trophic chain length -- Characterization -- Degradation -- Kinetic theory -- Mathematical models -- Nitrogen -- Phosphorus -- Recycling -- Surface waters -- Ecosystems -- biological model -- biomass -- bioremediation -- conference paper -- ecosystem -- food chain -- microclimate -- Biodegradation, Environmental -- Biomass -- Ecological Systems, Closed -- Ecosystem -- Food Chain -- Life Support Systems -- Models, Biological
Аннотация: In this paper, the influence of the extent of openness of ecosystem that is defined by the dilution rate, which characterizes the extent of flowage of the pond, on the intensity of the biotic circulation in ecosystems with different regulation types, number of trophic links and extent of closing has been investigated. We considered open systems, we took into account the return of the limiting substances, such as nitrogen and phosphorous, into the cycle by degradation of detritus and products of vital functions of consumers. It was shown by the numerical calculations that the increase of the dilution rate in without recycle ecosystems leads to increase of the net primary production up to the maximum value corresponding to the two-link trophic chain (biogenic substance and producer) and then, to gradually decrease. The residual concentration of biogenic limiting substances monotone increases. Net primary production and residual concentration of biogenic limiting substances in systems with recycle with even number of links behaves similarly to that in without recycle ecosystems. In the systems with recycle with the odd number of links that values lies on the stable level. We showed that in wide range of the dilution rate the recycling of the ecosystem can highly increase the net primary production and reduce residual concentration of biogenic limiting substances. The influence of the dilution rate on numbers of links that may exist in the system was analyzed. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Krasnoyarsk State University, Svobodny 79, Krasnoyarsk 660041, Russian Federation
Institute of Biophysics, SB, RAS, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Galayda, Ya.V.; Pechurkin, N.S.; Shirobokova, I.M.; Pisman, T.I.

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Characterization of polymeric microparticles based on resorbable polyesters of oxyalkanoic acids as a platform for deposition and delivery of drugs / A. V. Goreva [et al.] // Polym. Sci. Ser. A. - 2012. - Vol. 54, Is. 2. - P94-105, DOI 10.1134/S0965545X12020022. - Cited References: 33. - This work was supported by the program for Support of Leading Scientific Schools of the Russian Federation (project no. 11.G34.31.0013.2010, Biotechnology of New Biomaterials) and the program of integrated studies of the Presidium of the Siberian Branch, Russian Academy of Sciences (project no. 93). . - 12. - ISSN 0965-545X

РУБ Polymer Science
Рубрики:
IN-VITRO RELEASE
   POLYHYDROXYBUTYRATE MICROSPHERES
   BLENDS
   RIFAMPICIN
   BIOCOMPATIBILITY
   DEGRADATION
   FORMULATION
   COMPOSITE
   CARRIERS
   MODEL
Аннотация: The effect of the preparation technique (chemical composition of a polymer, type and method of emulsion mixing, and molecular mass of a drug) on the yield, structure, and size of microparticles obtained from resorbable polyesters of microbiological origin, polyhydroxyalkanoates, is studied. It is found that the concentration of the polymer solution and the method of emulsion mixing are the most significant factors affecting the diameter of microparticles based on polyhydroxyalkanoates; the surface structure of particles depends to a higher extent on the chemical composition of the polymer. The family of microparticles from 100-200 nm to 50-70 mu m in diameter is synthesized. It is shown that the rate of drug release from microparticles in vitro into the medium is higher in the case of 3-hydroxybutyrate copolymers with 3-hydroxyvalerate than in the case of the homopolymer of 3-hydroxybutyrate. This parameter increases with the content of 3-hydroxyvalerate units in the copolymer and the porosity and mass fraction of the drug in particles with a decrease in their sizes. For in vitro systems containing a phosphate buffer, variation in the preparation parameters makes it possible to obtain microparticles with various characteristics suitable for deposition of drugs. For microparticles obtained from polyhydroxyalkanoates and having different diameters, the mathematical description of the kinetics of drug release from the polymer matrix is provided.

Держатели документа:
[Goreva, A. V.
Shishatskaya, E. I.
Volova, T. G.] Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
[Shishatskaya, E. I.
Volova, T. G.] Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Krasnoyarsk 660041, Russia
[Goreva, A. V.
Sinskey, A. J.] MIT, Cambridge, MA 02139 USA : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Goreva, A.V.; Shishatskaya, E.I.; Volova, T.G.; Sinskey, A.J.

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Characterization of field emission cathodes with different forms of diamond coatings [Text] / V. V. Zhirnov [et al.] // J. Vac. Sci. Technol. B. - 1999. - Vol. 17: 11th International Vacuum Microelectronics Conference (IVMC 98) (JUL 19-23, 1998, ASHEVILLE, NORTH CAROLINA), Is. 2. - P666-669, DOI 10.1116/1.590614. - Cited References: 9 . - 4. - ISSN 1071-1023

РУБ Engineering, Electrical & Electronic + Nanoscience & Nanotechnology + Physics, Applied
Рубрики:
TIPS
   SILICON
   GROWTH
   ARRAYS
   FILMS
Аннотация: The emission of different, diamond coatings on silicon field emitter. arrays were investigated: chemical vapor deposition diamond, high pressure and high temperature synthetic diamond, and shock-synthesized nanodiamond. Practical emission characteristics such as: emission threshold, maximum current, current stability, and reproducibility were tested. The effects of surface modification, the size of diamond crystallites, and hydrogen plasma treatments were also studied. (C) 1999 American Vacuum Society. [S0734-211X(99)06102-8].

Держатели документа:
Inst Crystallog, Moscow 117333, Russia
Univ Fribourg, Dept Phys, CH-1700 Fribourg, Switzerland
Russian Fed Nucl Ctr, Snezhinsk 456770, Russia
Inst Biophys, Krasnoyarsk 660036, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zhirnov, V.V.; Kuttel, O.M.; Groning, O...; Alimova, A.N.; Detkov, P.Y.; Belobrov, P.I.; Maillard-Schaller, E...; Schlapbach, L...

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Ecological and floristic characterization of phytoplankton from small water bodies of Krasnoyarsk (Russia) / E. A. Ivanova, E. S. Kravchuk, O. V. Kolmakova // International Journal on Algae. - 2006. - Vol. 8, Is. 2. - P129-140, DOI 10.1615/InterJAlgae.v8.i2.20 . - ISSN 1521-9429
Кл.слова (ненормированные):
Biomass -- Divisions of algae -- Saprobility -- Trophic status -- algae -- Bacillariophyta -- Chlorophyta -- Cyanobacteria
Аннотация: The species composition of phytoplankton from four small recreation water bodies of Krasnoyarsk (ponds Bugach, Lesnoy, Nizhniy, and Vetluzhanka) is studied. The greatest taxonomic diversity is observed in Chlorophyta, Cyanophyta, and Bacillariophyta. The trophic status of the studied water bodies is determined. В©Begell House Inc., 2006.

Scopus
Держатели документа:
Krasnoyarsk State Agrarian University, Department of Botany and Plant Physiology, 88, Pr. Mira, 660049 Krasnoyarsk, Russian Federation
Institute of Biophysics, Siberian Branch, Academy of Sciences of Russia, 36, Akademgorodok, 660036 Krasnoyarsk, Russian Federation
Krasnoyarsk State University, Department of Biochemistry, 79, Pr. Svobodnyi, 660041 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Ivanova, E.A.; Kravchuk, E.S.; Kolmakova, O.V.

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Characterization of recombinant obelin as an intracellular Ca++ indicator [Text] / V. A. Illarionova [et al.] ; ed.: JW Hastings, LJ Kricka, J Kricka, // BIOLUMINESCENCE AND CHEMILUMINESCENCE: MOLECULAR REPORTING WITH PHOTONS : JOHN WILEY & SONS LTD, 1997. - 9th International Symposium on Bioluminescence and Chemiluminescence (OCT, 1996, WOODS HOLE, MA). - P427-430. - Cited References: 0 . - 4. - ISBN 0-471-97502-8

РУБ Biochemistry & Molecular Biology + Biophysics + Chemistry, Physical

: 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
Illarionova, V.A.; Illarionov, B.A.; Bondar, V.S.; Vysotski, E.S.; Blinks, J.R.; Hastings, JW \ed.\; Kricka, LJ \ed.\; Kricka,, J \ed.\

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High-active truncated luciferases of copepod Metridia longa and their characterization as secreted reporters inmammalian cells / S. V. Markova, L. P. Burakova, E. S. Vysotski // Luminescence. - 2012. - Vol. 27, Is. 2. - P138-139. - Cited References: 1 . - ISSN 1522-7235

РУБ Biochemistry & Molecular Biology

Держатели документа:
[Markova, Svetlana V.
Burakova, Ludmila P.
Vysotski, Eugene S.] Inst Biophys SB RAS, Photobiol Lab, Krasnoyarsk 660036, Russia
[Markova, Svetlana V.
Burakova, Ludmila P.
Vysotski, Eugene S.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
Markova, S.V.; Burakova, L.P.; Vysotski, E.S.

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Green-fluorescent protein from the bioluminescent jellyfish Clytia gregaria: cDNA cloning, expression, and characterization of novel recombinant protein [Text] / S. V. Markova [et al.] // Photochem. Photobiol. Sci. - 2010. - Vol. 9, Is. 6. - P757-765, DOI 10.1039/c0pp00023j. - Cited References: 42. - We thank Dr John Lee (University of Georgia) for constructive suggestions. This work was supported by the Russian Foundation for Basic Research (Grants: 08-04-92209 and 09-04-12022), "Molecular and Cell Biology" program of RAS, and Bayer AG (Germany). . - ISSN 1474-905X

РУБ Biochemistry & Molecular Biology + Biophysics + Chemistry, Physical
Рубрики:
ENERGY-TRANSFER
   CA2+-REGULATED PHOTOPROTEINS
   RENILLA BIOLUMINESCENCE
   ANGSTROM RESOLUTION
   SEQUENCE-ANALYSIS
   CRYSTAL-STRUCTURE
   EXCITED-STATE
   AEQUORIN
   PURIFICATION
   OBELIN
Аннотация: The bioluminescent systems of many marine organisms are comprised of two proteins - the Ca2+-regulated photoprotein and green-fluorescent protein (GFP). This work reports the cloning of the full-size cDNA encoding GFP (cgreGFP) from jellyfish Clytia gregaria, its expression and properties of the recombinant protein. The overall degree of identity between the amino acid sequence of the novel cgreGFP and the sequence of GFP (avGFP) from Aequorea victoria is 42% (similarity - 64%) despite these GFPs originating from jellyfish that both belong to the same class, Hydrozoa. However although the degree of identity is low, three residues, Ser-Tyr-Gly, which form the chromophore are identical in both GFPs. The cgreGFP displayed two absorption peaks at 278 and 485 nm, and the fluorescence maximum at 500 nm. The fluorescence quantum yield was determined to be 0.86, the brightness to be 54 mM(-1) cm(-1). For the first time we have also demonstrated an efficient radiationless energy transfer in vitro between clytin and cgreGFP in solution at micromolar concentrations. The cgreGFP may be a useful intracellular fluorescent marker, as it was able to be expressed in mammalian cells.

Держатели документа:
[Markova, Svetlana V.
Burakova, Ludmila P.
Frank, Ludmila A.
Korostileva, Kseniya A.
Vysotski, Eugene S.] Russian Acad Sci, Inst Biophys, Siberian Branch, Photobiol Lab, Krasnoyarsk 660036, Russia
[Markova, Svetlana V.
Frank, Ludmila A.
Korostileva, Kseniya A.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
[Golz, Stefan] Bayer Schering Pharma AG, BSP GDD GTR TD GT, D-42096 Wuppertal, Germany
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Markova, S.V.; Burakova, L.P.; Frank, L.A.; Golz, S...; Korostileva, K.A.; Vysotski, E.S.

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Expression, purification and characterization of the secreted luciferase of the copepod Metridia longa from Sf9 insect cells [Text] / G. A. Stepanyuk [et al.] // Protein Expr. Purif. - 2008. - Vol. 61, Is. 2. - P142-148, DOI 10.1016/j.pep.2008.05.013. - Cited References: 34. - This work was supported by the National Institutes of Health (Grant 1P50 GM62407), University of Georgia Research Foundation and Georgia Research Alliance, the Russian Foundation for Basic Research and Taiwan National Science Council (Grant 06-0489502) and the program for "Molecular and Cellular Biology" of Russian Academy of Sciences. . - ISSN 1046-5928

РУБ Biochemical Research Methods + Biochemistry & Molecular Biology + Biotechnology & Applied Microbiology
Рубрики:
VARGULA-HILGENDORFII LUCIFERASE
   CRYSTAL-STRUCTURE
   RENILLA-RENIFORMIS
   GAUSSIA LUCIFERASE
   BIOLUMINESCENT REPORTER
   OBELIN BIOLUMINESCENCE
   ANGSTROM RESOLUTION
   MAMMALIAN-CELLS
   GENE-EXPRESSION
   IN-VIVO
Аннотация: Metridia luciferase is a secreted luciferase from a marine copepod and uses coelenterazine as a substrate to produce a blue bioluminescence This luciferase has been successfully applied as a bioluminescent reporter in mammalian cells. The main advantage of secreted luciferase as a reporter is the capability of measuring intracellular events without destroying the cells or tissues and this property is well suited for development of high throughput screening technologies. However because Metridia luciferase is a Cys-rich protein, Escherichia coli expression systems produce an incorrectly folded protein, hindering its biochemical characterization and application for development of in vitro bioluminescent assays. Here we report the successful expression of Metridia luciferase with its signal peptide for secretion, in insect (Sf9) cells using the baculovirus expression system. Functionally active luciferase secreted by insect cells into the culture media has been efficiently purified with a yield of high purity protein of 2-3mg/L This Metridia luciferase expressed in the insect cell system is a monomeric protein showing 3.5-fold greater bioluminescence activity than luciferase expressed and purified from E. coli. The near coincidence of the experimental mass of Metridia luciferase purified from insect cells with that calculated from amino acid sequence. indicates that luciferase does not undergo post-translational modifications such as phosphorylation or glycosylation and also, the cleavage site of the signal peptide for secretion is at VQA-KS, as predicted from sequence analysis. (c) 2008 Elsevier Inc. All rights reserved.

Держатели документа:
[Stepanyuk, Galina A.
Markova, Svetlana V.
Vysotski, Eugene S.] Russian Acad Sci, Photobiol Lab, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
[Stepanyuk, Galina A.
Xu, Hao
Wu, Chia-Kuei
Lee, John
Vysotski, Eugene S.
Wang, Bi-Cheng] Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Stepanyuk, G.A.; Xu, H...; Wu, C.K.; Markova, S.V.; Lee, J...; Vysotski, E.S.; Wang, B.C.

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10.

Coelenterazine-binding protein of Renilla muelleri: cDNA cloning, overexpression, and characterization as a substrate of luciferase [Text] / M. S. Titushin [et al.] // Photochem. Photobiol. Sci. - 2008. - Vol. 7, Is. 2. - P189-196, DOI 10.1039/b713109g. - Cited References: 41 . - ISSN 1474-905X

РУБ Biochemistry & Molecular Biology + Biophysics + Chemistry, Physical
Рубрики:
CRYSTAL-STRUCTURE
   LIGHT-EMISSION
   CA2+-REGULATED PHOTOPROTEINS
   BIOLUMINESCENT REPORTER
   RENIFORMIS LUCIFERASE
   ANGSTROM RESOLUTION
   RECOMBINANT OBELIN
   ENERGY-TRANSFER
   EXCITED-STATE
   CALCIUM
Аннотация: The Renilla bioluminescent system in vivo is comprised of three proteins-the luciferase, green-fluorescent protein, and coelenterazine-binding protein (CBP), previously called luciferin-binding protein (LBP). This work reports the cloning of the full-size cDNA encoding CBP from soft coral Renilla muelleri, its overexpression and properties of the recombinant protein. The apo-CBP was quantitatively converted to CBP by simple incubation with coelenterazine. The physicochemical properties of this recombinant CBP are determined to be practically the same as those reported for the CBP (LBP) of R. reniformis. CBP is a member of the four-EF-hand Ca2+-binding superfamily of proteins with only three of the EF-hand loops having the Ca2+-binding consensus sequences. There is weak sequence homology with the Ca2+-regulated photoproteins but only as a result of the necessary Ca2+-binding loop structure. In combination with Renilla luciferase, addition of only one Ca2+ is sufficient to release the coelenterazine as a substrate for the luciferase for bioluminescence. This combination of the two proteins generates bioluminescence with higher reaction efficiency than using free coelenterazine alone as the substrate for luciferase. This increased quantum yield, a difference of bioluminescence spectra, and markedly different kinetics, implicate that a CBP-luciferase complex might be involved.

Держатели документа:
[Titushin, Maxim S.
Markova, Svetlana V.
Frank, Ludmila A.
Malikova, Natalia P.
Stepanyuk, Galina A.
Vysotski, Eugene S.] Russian Acad Sci, Siberian Branch, Inst Biophys, Photobiol Lab, Krasnoyarsk 660036, Russia
[Lee, John
Vysotski, Eugene S.] Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Titushin, M.S.; Markova, S.V.; Frank, L.A.; Malikova, N.P.; Stepanyuk, G.A.; Lee, J...; Vysotski, E.S.

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11.

Recombinant obelin: Cloning and expression of cDNA, purification, and characterization as a calcium indicator [Text] / B. A. Illarionov [et al.] // Methods Enzymol. - 2000. - Vol. 305. - P223-249. - Cited References: 58 . - ISSN 0076-6879

РУБ Biochemical Research Methods + Biochemistry & Molecular Biology
Рубрики:
PHOTOPROTEIN OBELIN
   MESSENGER-RNA
   CA-2+-ACTIVATED PHOTOPROTEIN
   DIRECTED MUTAGENESIS
   SEQUENCE-ANALYSIS
   HYDROID OBELIA
   AEQUORIN
   PROTEIN
   BIOLUMINESCENCE
   LUMINESCENCE

Держатели документа:
Russian Acad Sci, Inst Biophys, Photobiol Lab, Krasnoyarsk 660036, Russia
Univ Washington, Friday Harbor Labs, Friday Harbor, WA 98250 USA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Illarionov, B.A.; Frank, L.A.; Illarionova, V.A.; Bondar, V.S.; Vysotski, E.S.; Blinks, J.R.

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12.

Purification and partial spectral characterization of a novel luciferin from the luminous enchytraeid Fridericia heliota / V. N. Petushkov, N. S. Rodionova // Journal of Photochemistry and Photobiology B: Biology. - 2007. - Vol. 87, Is. 2. - P130-136, DOI 10.1016/j.jphotobiol.2007.03.006 . - ISSN 1011-1344
Кл.слова (ненормированные):
ATP -- Bioluminescence -- Earthworms -- Enchytraeid -- Luciferase -- Luciferin -- adenosine triphosphate -- luciferase -- luciferin -- animal cell -- anion exchange chromatography -- annelid worm -- article -- controlled study -- earthworm -- firefly -- luminance -- luminescence -- nonhuman -- priority journal -- protein analysis -- protein purification -- radiation absorption -- reversed phase liquid chromatography -- ultraviolet radiation -- Adenosine Triphosphate -- Animals -- Luciferases -- Luminescent Agents -- Oligochaeta -- Spectrum Analysis -- Enchytraeidae -- Fridericia heliota
Аннотация: A homogeneous luciferin preparation has been obtained from the luminous soil enchytraeid Fridericia heliota, which has an ATP-dependent luminescent system. A procedure for luciferin purification without losing fractions of active luciferase has been developed. The luciferin specific activity is 4000 times increased; its UV absorption spectrum maximum is 294 nm with a local minimum at 262 nm. The luciferin of the enchytraeid F. heliota is significantly different from firefly luciferin, whose luminescent reaction also requires ATP, and it also appears to have no similarities to other known luciferins. В© 2007 Elsevier B.V. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V.N.; Rodionova, N.S.

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13.

Redescription of Fridericia heliota (Annelida, Clitellata: Enchytraeidae), a luminous worm from the Siberian taiga, with a review of bioluminescence in the Oligochaeta / E. Rota [et al.] // Journal of Zoology. - 2003. - Vol. 260, Is. 3. - P291-299, DOI 10.1017/S0952836903003777 . - ISSN 0952-8369
Кл.слова (ненормированные):
Bioluminescence -- Enchytraeidae -- Fridericia heliota -- Siberian taiga -- Taxonomy -- annelid -- bioluminescence -- ecomorphology -- taxonomy -- Russian Federation -- Annelida -- Clitellata -- Enchytraeidae -- Hirudinea -- Invertebrata -- Oligochaeta (Metazoa)
Аннотация: The Siberian enchytraeid Fridericia heliota Zalesskaja, 1990 has so far been known only through a paper in Russian briefly describing its luminescence and the major external and internal morphological features. The present study expands that preliminary description, amending it as regards the chaetal formula, the clitellar pattern, the form of the nephridia and the occurrence of glands at the spermathecal orifices, and providing a more comprehensive taxonomic characterization of the species. More detailed information is also given on its habitat and geographical range as well as on its unusual luminous properties. In F. heliota, the production of light is confined to the body wall with a pattern corresponding to that of the epidermal gland cells, although it does not involve discharge of luminous mucus. This contrasts with observations on other enchytraeids and on the oligochaetes in general. Records of luminosity in the Oligochaeta are reviewed with comments.

Scopus
Держатели документа:
Dipartimento di Scienze Ambientali, Sezione Sist. Ecologia Anim. e Veg., Universita di Siena, Via P. A. Mattioli 4, IT-53100 Siena, Italy
Inst. Ecol./Evol. Prob. A. N. S., Russian Academy of Sciences, Leninskij Prosp. 33, Moscow B-71, 117071, Russian Federation
Laboratory of Photobiology, Institute of Biophysics, Russian Academy of Sciences, Krasnoyarsk-36, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Rota, E.; Zalesskaja, N.T.; Rodionova, N.S.; Petushkov, V.N.

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14.

Purification and characterization of flavoproteins and cytochromes from the yellow bioluminescence marine bacterium Vibrio fischeri strain Y1 / V. N. Petushkov, J. Lee // European Journal of Biochemistry. - 1997. - Vol. 245, Is. 3. - P790-796 . - ISSN 0014-2956
Кл.слова (ненормированные):
anisotropy -- lumazine protein -- Photobacterium -- thioredoxin reductase -- time-resolved fluorescence -- cytochrome -- flavoprotein -- article -- bioluminescence -- nonhuman -- priority journal -- protein analysis -- protein purification -- sea -- vibrio -- Amino Acid Sequence -- Bacterial Proteins -- Cytochromes -- Flavoproteins -- Molecular Sequence Data -- Sequence Alignment -- Vibrio -- Azotobacter -- Bacteria (microorganisms) -- Escherichia coli -- Haemophilus -- haemophilus influenza -- Murinae -- Negibacteria -- Photobacterium -- Photobacterium leiognathi -- Pseudomonas -- uncultured marine bacterium -- Vibrio fischeri
Аннотация: Several flavoproteins and cytochromes that occur as major components in extracts of the yellow bioluminescence Y1 strain of the murine bacterium Vibrio fischeri have been purified and characterized with respect to their mass (SDS/PAGE) and matrix-assisted laser-desorption/ionization MS), chromatographic properties, N-terminal sequence, and spectroscopy (absorption, fluorescence emission and anisotropy decay). The investigated proteins were as follows: yellow fluorescence protein (YFP) with bound riboflavin, FMN or 6,7-dimethyl-8-ribityllumazine; a blue fluorescence protein (BFP) with bound 6,7-dimethyl-8-ribityllumazine, riboflavin, or 6- methyl-7-oxo-ribityllumazine; thioredoxin reductase with FAD as ligand; and two c-type diheme cytochromes, c551 and c554. We present evidence that the riboflavin-bound YFP has an N-terminal sequence corresponding to that published for the dimeric YFP. We show that an equilibrium replacement of the riboflavin can be made with excess lumazine derivative and that lumazine- bound YFP has different bioluminescence properties to those of the lumazine protein from Photobacterium leiognathi. BFP is a different protein again, and in the bacterial lysate it occurs in multiple forms, ligated to either riboflavin, lumazine, or t he 7-oxolumazine derivative. The N-terminal sequence for BFP-shows similarities to those of the YFP proteins and to lumazine protein and riboflavin synthase from Photobacterium. BFP in any form has no bioluminescence or riboflavin-synthase activity. A 70-kDa fluorescent flavoprotein with FAD as ligand has an N-terminal sequence highly similar to those of thioredoxin reductases from Haemophilus influenza and Escherichia coli. Cytochrome contaminations in previous preparations of YFP have been removed and an identified as the two c-type cytochromes c551 and c554. Both inhibit the NADH-induced bioluminescence in the reductase/luciferase system with the luciferase from P. leiognathi and V. fischeri. The N-terminal amino acid sequence of the cytochrome (c551) corresponds to a diheme cytochrome c4. The spectral properties of c554 are similar to those of other c5 cytochromes, and both c554 and c551 have absorption spectra similar to those of the respective cytochromes from the gram-negative bacteria Pseudomonas and Azotobacter.

Scopus
Держатели документа:
Dept. of Biochem. and Molec. Biology, University of Georgia, Athens, GA, United States
Institute of Biophysics, Academy of Sciences of Russia, Krasnoyarsk, Russian Federation
Dept. of Biochem. and Molec. Biology, University of Georgia, Athens, GA 30602, United States : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V.N.; Lee, J.

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15.

A glucose-utilizing strain, cupriavidus euthrophus B-10646: Growth kinetics, characterization and synthesis of multicomponent PHAs / T. Volova [et al.] // PLoS ONE. - 2014. - Vol. 9, Is. 2, DOI 10.1371/journal.pone.0087551 . - ISSN 1932-6203
Кл.слова (ненормированные):
3 hydroxybutyrate 3 hydroxyhexanoate 3 hydroxyvalerate copolymer -- 3 hydroxybutyrate 4 hydroxybutyrate 3 hydroxyvalerate copolymer -- copolymer -- gamma butyrolactone -- glucose -- hexanoic acid -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- polystyrene -- propionic acid -- unclassified drug -- valeric acid -- animal cell -- article -- bacterial growth -- bacterium culture -- cell adhesion -- cell proliferation -- crystal structure -- culture optimization -- Cupriavidus -- Cupriavidus euthrophus -- decomposition -- elasticity -- film -- glucose utilization -- kinetics -- mechanics -- melting point -- mouse -- nonhuman -- nucleotide sequence -- physical chemistry -- polymerization -- strength -- synthesis
Аннотация: This study investigates kinetic and production parameters of a glucose-utilizing bacterial strain, C. eutrophus B-10646, and its ability to synthesize PHA terpolymers. Optimization of a number of parameters of bacterial culture (cell concentration in the inoculum, physiological activity of the inoculum, determined by the initial intracellular polymer content, and glucose concentration in the culture medium during cultivation) provided cell concentrations and PHA yields reaching 110 g/L and 80%, respectively, under two-stage batch culture conditions. Addition of precursor substrates (valerate, hexanoate, propionate, ?-butyrolactone) to the culture medium enabled synthesis of PHA terpolymers, P(3HB/3HV/4HB) and P(3HB/ 3HV/3HHx), with different composition and different molar fractions of 3HB, 3HV, 4HB, and 3HHx. Different types of PHA terpolymers synthesized by C. eutrophus B-10646 were used to prepare films, whose physicochemical and physical-mechanical properties were investigated. The properties of PHA terpolymers were significantly different from those of the P3HB homopolymer: they had much lower degrees of crystallinity and lower melting points and thermal decomposition temperatures, with the difference between these temperatures remaining practically unchanged. Films prepared from all PHA terpolymers had higher mechanical strength and elasticity than P3HB films. In spite of dissimilar surface structures, all films prepared from PHA terpolymers facilitated attachment and proliferation of mouse fibroblast NIH 3T3 cells more effectively than polystyrene and the highly crystalline P3HB. Copyright: © 2014 Volova et al.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Science, Moscow, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.; Kiselev, E.; Vinogradova, O.; Nikolaeva, E.; Chistyakov, A.; Sukovatiy, A.; Shishatskaya, E.

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16.

Characterization of hydromedusan Ca2+-regulated photoproteins as a tool for measurement of Ca2+concentration / N. P. Malikova [et al.] // . - 2014, DOI 10.1007/s00216-014-7986-2 . - ISSN 1618-2642
Кл.слова (ненормированные):
Aequorin -- Calcium -- Clytin -- Coelenterazine -- Mitrocomin -- Obelin
Аннотация: Calcium ion is a ubiquitous intracellular messenger, performing this function in many eukaryotic cells. To understand calcium regulation mechanisms and how disturbances of these mechanisms are associated with disease states, it is necessary to measure calcium inside cells. Ca2+-regulated photoproteins have been successfully used for this purpose for many years. Here we report the results of comparative studies on the properties of recombinant aequorin from Aequorea victoria, recombinant obelins from Obelia geniculata and Obelia longissima, recombinant mitrocomin from Mitrocoma cellularia, and recombinant clytin from Clytia gregaria as intracellular calcium indicators in a set of identical in vitro and in vivo experiments. Although photoproteins reveal a high degree of identity of amino acid sequences and spatial structures, and, apparently, have a common mechanism for the bioluminescence reaction, they were found to differ in the Ca2+ concentration detection limit, the sensitivity of bioluminescence to Mg2+, and the rates of the rise of the luminescence signal with a sudden change of Ca2+ concentration. In addition, the bioluminescence activities of Chinese hamster ovary cells expressing wild-type photoproteins also differed. The light signals of cells expressing mitrocomin, for example, slightly exceeded the background, suggesting that mitrocomin may be hardly used to detect intracellular Ca2+ without modifications improving its properties. On the basis of experiments on the activation of endogenous P2Y2 receptor in Chinese hamster ovary cells by ATP, we suggest that wild-type aequorin and obelin from O. longissima are more suitable for calcium detection in cytoplasm, whereas clytin and obelin from O. geniculata can be used for calcium measurement in cell compartments with high Ca2+ concentration. [Figure not available: see fulltext.] © 2014 Springer-Verlag Berlin Heidelberg.

Scopus
Держатели документа:
Photobiology Laboratory, Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, 660036, Russian Federation
Laboratory of Bioluminescent Biotechnologies, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Malikova, N.P.; Burakova, L.P.; Markova, S.V.; Vysotski, E.S.

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17.

A Glucose-Utilizing Strain, Cupriavidus euthrophus B-10646: Growth Kinetics, Characterization and Synthesis of Multicomponent PHAs [Text] / T. . Volova [et al.] // PLoS One. - 2014. - Vol. 9, Is. 2. - Ст. e87551, DOI 10.1371/journal.pone.0087551. - Cited References: 64. - This study was financially supported by Project "Biotechnologies of novel biomaterials: Innovative Biopolymers and Biomedicine Devices" (Agreement No. 11.G34.31.0013 with Amendment No. 1 of 15 February 2013) in accordance with Resolution No. 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning." The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. . - ISSN 1932-6203

РУБ Multidisciplinary Sciences
Рубрики:
RALSTONIA-EUTROPHA
   BIODEGRADABLE POLYHYDROXYALKANOATES
   AEROMONAS-HYDROPHILA
   ESCHERICHIA-COLI
   MOLECULAR-WEIGHT
   SURFACE-ENERGY
   NORTH PACIFIC
   TERPOLYESTER
   BIOSYNTHESIS
   POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE-CO-3-HYDROXYHEXANOATE)
Аннотация: This study investigates kinetic and production parameters of a glucose-utilizing bacterial strain, C. eutrophus B-10646, and its ability to synthesize PHA terpolymers. Optimization of a number of parameters of bacterial culture (cell concentration in the inoculum, physiological activity of the inoculum, determined by the initial intracellular polymer content, and glucose concentration in the culture medium during cultivation) provided cell concentrations and PHA yields reaching 110 g/L and 80%, respectively, under two-stage batch culture conditions. Addition of precursor substrates (valerate, hexanoate, propionate, c-butyrolactone) to the culture medium enabled synthesis of PHA terpolymers, P(3HB/3HV/4HB) and P(3HB/3HV/3HHx), with different composition and different molar fractions of 3HB, 3HV, 4HB, and 3HHx. Different types of PHA terpolymers synthesized by C. eutrophus B-10646 were used to prepare films, whose physicochemical and physicalmechanical properties were investigated. The properties of PHA terpolymers were significantly different from those of the P3HB homopolymer: they had much lower degrees of crystallinity and lower melting points and thermal decomposition temperatures, with the difference between these temperatures remaining practically unchanged. Films prepared from all PHA terpolymers had higher mechanical strength and elasticity than P3HB films. In spite of dissimilar surface structures, all films prepared from PHA terpolymers facilitated attachment and proliferation of mouse fibroblast NIH 3T3 cells more effectively than polystyrene and the highly crystalline P3HB.

WOS
Держатели документа:
[Volova, Tatiana
Kiselev, Evgeniy
Nikolaeva, Elena
Sukovatiy, Aleksey
Shishatskaya, Ekaterina] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia
[Volova, Tatiana
Vinogradova, Olga
Shishatskaya, Ekaterina] Siberian Fed Univ, Krasnoyarsk, Russia
[Chistyakov, Anton] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T...; Kiselev, E...; Vinogradova, O...; Nikolaeva, E...; Chistyakov, A...; Sukovatiy, A...; Shishatskaya, E...; Project "Biotechnologies of novel biomaterials: Innovative Biopolymers and Biomedicine Devices" [11.G34.31.0013]

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18.

Characterization of hydromedusan Ca2+-regulated photoproteins as a tool for measurement of Ca(2+)concentration [Text] / N. P. Malikova [et al.] // Anal. Bioanal. Chem. - 2014. - Vol. 406, Is. 23. - P5715-5726, DOI 10.1007/s00216-014-7986-2. - Cited References: 67. - This work was supported by RFBR grant 12-04-00131, by the programs of the Government of the Russian Federation "Measures to Attract Leading Scientists to Russian Educational Institutions" (grant 11.G34.31.0058) and "Molecular and Cellular Biology" of the Russian Academy of Sciences, and the grant from the President of the Russian Federation "Leading Science School" (3951.2012.4). . - ISSN 1618-2642. - ISSN 1618-2650

РУБ Biochemical Research Methods + Chemistry, Analytical
Рубрики:
LIGHT-SENSITIVE PHOTOPROTEIN
   CTENOPHORE BEROE ABYSSICOLA
   GREEN-FLUORESCENT PROTEIN
   INTRACELLULAR CALCIUM
   SEQUENCE-ANALYSIS
   CA-2+-ACTIVATED PHOTOPROTEIN
   CA2+-BINDING PHOTOPROTEIN
   SEMISYNTHETIC AEQUORINS
   LUMINESCENT PROTEIN
   RECOMBINANT OBELIN
Кл.слова (ненормированные):
Calcium -- Coelenterazine -- Aequorin -- Obelin -- Clytin -- Mitrocomin
Аннотация: Calcium ion is a ubiquitous intracellular messenger, performing this function in many eukaryotic cells. To understand calcium regulation mechanisms and how disturbances of these mechanisms are associated with disease states, it is necessary to measure calcium inside cells. Ca2+-regulated photoproteins have been successfully used for this purpose for many years. Here we report the results of comparative studies on the properties of recombinant aequorin from Aequorea victoria, recombinant obelins from Obelia geniculata and Obelia longissima, recombinant mitrocomin from Mitrocoma cellularia, and recombinant clytin from Clytia gregaria as intracellular calcium indicators in a set of identical in vitro and in vivo experiments. Although photoproteins reveal a high degree of identity of amino acid sequences and spatial structures, and, apparently, have a common mechanism for the bioluminescence reaction, they were found to differ in the Ca2+ concentration detection limit, the sensitivity of bioluminescence to Mg2+, and the rates of the rise of the luminescence signal with a sudden change of Ca2+ concentration. In addition, the bioluminescence activities of Chinese hamster ovary cells expressing wild-type photoproteins also differed. The light signals of cells expressing mitrocomin, for example, slightly exceeded the background, suggesting that mitrocomin may be hardly used to detect intracellular Ca2+ without modifications improving its properties. On the basis of experiments on the activation of endogenous P2Y(2) receptor in Chinese hamster ovary cells by ATP, we suggest that wild-type aequorin and obelin from O. longissima are more suitable for calcium detection in cytoplasm, whereas clytin and obelin from O. geniculata can be used for calcium measurement in cell compartments with high Ca2+ concentration.

WOS
Держатели документа:
[Malikova, Natalia P.
Burakova, Ludmila P.
Markova, Svetlana V.
Vysotski, Eugene S.] Russian Acad Sci, Inst Biophys, Siberian Branch, Photobiol Lab, Krasnoyarsk 660036, Russia
[Malikova, Natalia P.
Burakova, Ludmila P.
Markova, Svetlana V.
Vysotski, Eugene S.] Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Lab Bioluminescent Biotechnol, Krasnoyarsk 660041, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Malikova, N.P.; Burakova, L.P.; Markova, S.V.; Vysotski, E.S.; RFBR [12-04-00131]; Government of the Russian Federation [11.G34.31.0058]; Russian Academy of Sciences; Russian Federation "Leading Science School" [3951.2012.4]

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19.

Green flavoprotein from P. leiognathi: purification, characterization and identification as the product of the lux G(N) gene / A. A. Raibekas // Journal of bioluminescence and chemiluminescence. - 1991. - Vol. 6, Is. 3. - P. 169-176 . - ISSN 0884-3996
Кл.слова (ненормированные):
bacterial protein -- flavoprotein -- amino acid sequence -- article -- bacterial gene -- chemistry -- genetics -- isolation and purification -- luminescence -- molecular genetics -- molecular weight -- Photobacterium -- Amino Acid Sequence -- Bacterial Proteins -- Flavoproteins -- Genes, Bacterial -- Luminescence -- Molecular Sequence Data -- Molecular Weight -- Photobacterium -- Support, U.S. Gov't, P.H.S.
Аннотация: A green flavoprotein (GFP) was isolated and purified to homogeneity from Photobacterium leiognathi, strain 208. GFP is a homodimer of molecular weight 54,000 and contains two molecules of an unusual flavin per molecule of protein. Various biochemical characteristics including isoelectric point, trypsin and chymotrypsin degradation, SDS and temperature influence on subunit dissociation and the dissociation of the flavin chromophore, were investigated. The sequence of 23 N-terminal amino acids was determined and found to be concurrent with the N-terminal amino acid sequence encoded by the lux G(N) gene of P. leiognathi. This fact suggests that GFP is a structural component of the Photobacterium luminescence system.

Scopus
Держатели документа:
Institute of Biophysics, USSR Academy of Sciences, Krasnoyarsk. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Raibekas, A.A.

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20.

Comparative characterization of the growth of recombinant Escherichia coli strains and species of the aboriginal microflora of aquatic microecosystems under selective and nonselective conditions [Текст] / T. V. Kargatova [и др.] // Izv. Akad. Nauk Ser. Biol. - 1999. - Is. 2. - P. 152-157. - Cited References: 9 . - ISSN 0002-3329

РУБ Biology
Рубрики:
GENETICALLY-MODIFIED MICROORGANISMS
Аннотация: We have studied the kinetic characteristics of several isolates of Escherichia coli Z905 recombinant strain after introduction of the strain into model aquatic ecosystems. Most E. coli Z905 isolates grown as batch cultures under selective conditions (0.5 mu g/ml ampicillin) showed better kinetic characteristics of growth than did related species of native microflora, which originally populated laboratory microcosms.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kargatova, T.V.; Maksimova, E.E.; Krylova, T.Y.; Popova, L.Y.

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