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Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН (3)

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Cytogenetic studies on submerged plants from the Yenisei River area in the zone of radioactive contamination [Text] / E. N. Muratova [et al.] // Biol. Bull. - 2014. - Vol. 41, Is. 5. - P461-467, DOI 10.1134/S1062359014050094. - Cited References: 21. - This work was supported by integration projects of the Siberian Branch of the Russian Academy of Sciences nos. 1, 30, 96. . - ISSN 1062-3590. - ISSN 1026-3470

РУБ Biology
Рубрики:
SOMATIC-CELLS
MUTATIONS
Аннотация: Cytogenetic studies on three species of submerged plants from different parts of the Yenisei River area subjected to radioactive impact of the Krasnoyarsk Mining-and-Chemical Plant and the Electrochemical Factory have been conducted. A high level of irregularities in ana-telophase and metaphase of mitoses has been revealed in test samples compared to the control: agglutination and fragmentation of chromosomes, lagging chromosomes, bridges, fragments, misdivisions, and others. The nature of the disorders indicates that they are related in part to the direct damage to the chromosome structure and in part to damage to the spindle.

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Держатели документа:
[Muratova, E. N.
Goryachkina, O. V.
Pimenov, A. V.
Sedelnikova, T. S.] Russian Acad Sci, Siberian Branch, Sukachev Inst Forestry, Krasnoyarsk 660036, Russia
[Kornilova, M. G.] Krasnoyarsk State Agr Univ, Krasnoyarsk 660049, Russia
[Bolsunovsky, A. Ya.] Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИЛ СО РАН
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Muratova, E.N.; Goryachkina, O.V.; Kornilova, M.G.; Pimenov, A.V.; Sedelnikova, T.S.; Bolsunovsky, A.Y.; Siberian Branch of the Russian Academy of Sciences [30, 96]

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Use of the aquatic plant Elodea canadensis to assess toxicity and genotoxicity of Yenisei River sediments / T. A. Zotina [et al.] // Environ. Toxicol. Chem. - 2015. - Vol. 34, Is. 10. - P2310-2321, DOI 10.1002/etc.3057 . - ISSN 0730-7268
Кл.слова (ненормированные):
Aquatic plants -- Biomarkers -- Genotoxicity -- Sediment quality -- Sediment toxicity -- Biomarkers -- Chromosomes -- Cytotoxicity -- Pollution -- Pollution control -- Radioactive waste disposal -- Radioactivity -- River pollution -- Sediments -- Toxicity -- Aquatic plants -- Genotoxicities -- Laboratory bioassay -- Radioactive contamination -- Radioactive pollution -- Sediment quality -- Sediment toxicity -- Toxicity endpoints -- Rivers -- Article -- bioassay -- controlled study -- cytotoxicity -- Elodea canadensis -- environmental exposure -- genotoxicity -- indicator organism -- lake sediment -- mitosis index -- nonhuman -- plant growth -- plant root -- priority journal -- radioactive pollution -- river -- root length -- Russian Federation -- sensitivity analysis -- shoot -- toxicity testing -- Elodea -- Elodea canadensis
Аннотация: The toxicity, cytotoxicity, and genotoxicity of bulk sediments from the Yenisei River (Siberia, Russia) were estimated in laboratory bioassays based on several endpoints in the aquatic plant Elodea canadensis. The bottom sediment samples were collected in the Yenisei River upstream and downstream of the sources of chemical and radioactive contamination. The testing revealed different sensitivities of Elodea endpoints to the quality of the bottom sediment: weight of shoots
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Держатели документа:
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Zotina, T. A.; Trofimova, E. A.; Medvedeva, M. Y.; Dementyev, D. V.; Bolsunovsky, A. Y.

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539.1.047:576.356
С 71


    Спектр хромосомных аберраций в корневой меристеме E. Canadensis из районов реки Енисей с разными типами техногенного загрязнения [Текст] / Институт биофизики СО РАН // Экологическая генетика. - 2016. - Том XIV, № 2. - С. 57-66DOI 10.17816/ecogen14257-66
   Перевод заглавия: Types of cytogenetic abnormalities in apical root meristem of Elodea сanadensis from Yenisei River areas with different types of anthropogenic pollution

УДК

539.1.047:576.356

Рубрики:
Хромосомные аберрации
Кл.слова (ненормированные):
генотоксичность -- аномалии митоза -- элодея канадская -- мосты -- агглютинация -- фрагментация хромосом
Аннотация: Показано изменение спектра хромосомных аномалий в корневой меристеме Elodea canadensis Michx. (элодея канадская), произрастающей в районах с разными типами техногенного загрязнения (радиационного и химического) донных отложений реки Енисей. В пробах элодеи, собранной в зоне радиоактивного загрязнения реки сбросами Горно-химического комбината Росатома (г. Железногорск), преобладающим типом аномалий были хромосомные аберрации (мосты, агглютинация, фрагментация хромосом), а также множественные аномалии в одной клетке. В пробах элодеи из районов реки Енисей с нерадиоактивным (химическим) загрязнением донных отложений предприятиями г. Красноярска преобладали аномалии, связанные с нарушением митотического веретена деления клетки.
The spectrum of cytogenetic abnormalities in the apical root meristem of Elodea сanadensis Michx. (elodea) from the Yenisei River was studied depending on the type of anthropogenic pollution. We compared elodea samples from areas with radiation-contaminated sediments (area affected by Rosatom Mining and Chemical Combine, MCC, Zheleznogorsk) and areas with chemical pollution (Krasnoyarsk). Materials and methods. The apical root meristem of elodea was fixed in acetic alcohol (96 % ethanol and glacial acetic acid, 3 : 1) and stained with 1 % aceto-orcein. The cytogenetic analysis of metaphase and ana-telophase elodea cells was carried out with temporal squashed preparations using an Olympus CX31 microscope. Results. At metaphase stage, the predominant types of abnormalities in elodea cells were disoriented chromosomes (up to 9 % of total metaphase cells) and chromosome agglutination (6 %). In the area affected by the MCC an increased content of ring chromosomes in elodea cells was detected, but in terms of frequency of their occurrence no significant differences were revealed between samples from areas with different types of pollution. Among abnormalities at ana-telophase stage, bridges (to 20% of dividing cells) and disoriented chromosomes (up to 8%) dominated. The following abnormalities were also detected: multipolar and asymmetrical mitoses, agglutination and cells with multiple chromosome abnormalities. It was shown that in areas with the highest content of 137Cs in bottom sediments the frequency of cells with bridges and multiple chromosome abnormalities significantly increased as compared to samples from other areas. Conclusion. In the radioactive contamination area of the Yenisei River the spectrum of abnormalities was dominated by chromosome aberrations (bridges, agglutination, chromosome fragmentation) and multiple abnormalities in one and the same cell. In areas with non-radioactive (chemical) contamination of sediments, mitotic spindle irregularities prevailed

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Держатели документа:
Институт биофизики Сибирского отделения Российской академии наук : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Медведева, Марина Юрьевна; Болсуновский, Александр Яковлевич; Институт биофизики Сибирского отделения Российской Академии наук (Красноярск)

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Sex Determination Model in Pink Salmon Oncorhynchus gorbuscha (Walbaum, 1792) (Salmonidae, Osteichthyes) Controlled by Multi-Copy Genes Located in Sex Chromosomes / A. A. Makhrov [et al.] // Dokl. Biochem. Biophys. - 2018. - Vol. 478, Is. 1. - P21-24, DOI 10.1134/S1607672918010064. - Cited References:14. - This work was supported by the Russian Science Foundation (project no. 16-14-10001). . - ISSN 1607-6729. - ISSN 1608-3091

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
FISH
Аннотация: This article is devoted to presenting the hypothesis explaining the fact of a considerable prevalence of phenotypic males among the triploid pink salmon as well as the regular occurrence of intersexes, which were revealed by us. This hypothesis also explains the large proportion (in some cases) in pink salmon populations of the individuals whose genetic sex does not match the phenotypic sex. We assume that the genes encoding the factors that contribute to the transformation of individuals into males (but not the marker sequences of the Y chromosome) are present not only in the Y chromosome of pink salmon but also in the X chromosome, although in smaller quantities.

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Держатели документа:
Russian Acad Sci, Severtsov Inst Ecol & Evolut, Leninskii Pr 33, Moscow 119071, Russia.
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk Res Ctr, Krasnoyarsk 660036, Russia.
Moscow MV Lomonosov State Univ, Fac Biol, Moscow 119991, Russia.

Доп.точки доступа:
Makhrov, A. A.; Artamonova, V. S.; Kolmakova, O. V.; Ponomareva, M. V.; Russian Science Foundation [16-14-10001]

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Cytogenetic Effects of -Radiation in Onion (Allium cepa L.) Seedlings / A. Y. Bolsunovsky [et al.] // Dokl. Biochem. Biophys. - 2018. - Vol. 481, Is. 1. - P181-185, DOI 10.1134/S1607672918040014. - Cited References:15 . - ISSN 1607-6729. - ISSN 1608-3091

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
YENISEI RIVER
CELLS
Аннотация: The effect of -radiation on the cytogenetic parameters of root meristem cells of onion seedlings was studied in laboratory experiments (Allium-test). An increase in the overall frequency of chromosomal aberrations and micronucleus frequencies in seedling cells at low -radiation doses (0.1 Gy) was detected for the first time. At a maximum absorbed dose of 13 Gy, chromosomal aberrations were detected in the majority of cells in the anaphase and telophase stages of the cell cycle, and the number of cells with multiple aberrations increased. The main contribution to the overall frequency of chromosomal aberrations, in addition to multiple aberrations, is made by the bridge-type aberrations, fragments, and lagging chromosomes. The data obtained allow using the cytogenetic indices of Allium cepa seedlings to assess the biological effects of lowdose -radiation.

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Держатели документа:
Russian Acad Sci, Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys,Siberian Branch, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Budker Inst Nucl Phys, Siberian Branch, Novosibirsk 630090, Russia.

Доп.точки доступа:
Bolsunovsky, A. Ya.; Dementyev, D. V.; Trofimova, E. A.; Iniatkina, E. M.; Kladko, Yu. V.; Petrichenkov, M. V.

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Chromosomal aberrations and micronuclei induced in onion (Allium cepa) by gamma-radiation / A. Bolsunovsky [et al.] // J. Environ. Radioact. - 2019. - Vol. 207. - P1-6, DOI 10.1016/j.jenvrad.2019.05.014 . - ISSN 0265-931X
Кл.слова (ненормированные):
Allium cepa -- Chromosomal aberrations -- Gamma-radiation -- Germinating seeds -- Low doses -- Micronuclei -- The dose-response curve -- Chromosomes -- Gamma rays -- Allium cepa -- Chromosomal aberration -- Dose-response curves -- Germinating seeds -- Low dose -- Micronuclei -- Radiation effects -- Allium -- Allium cepa
Аннотация: The Allium-test is commonly used to assess genotoxicity of chemical and physical factors. In the present study, the roots of germinating onion (Allium cepa) were exposed to 0.02–13 Gy of ?-radiation. The dose dependencies of the frequency of chromosomal aberrations and micronuclei were nonlinear. An increase in the frequency of chromosomal aberrations in germinating seed root cells was first found under exposure to low doses of ?-radiation (0.05 and 0.1 Gy). Micronuclei inductions at low doses of radiation were not significantly different from the control. Our study suggests that germinating onion seed roots are a sensitive bioassay material for assessing the genotoxic effects of low-dose ?-radiation. © 2019 Elsevier Ltd

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Держатели документа:
Institute of Biophysics Siberian Branch of Russian Academy of Sciences, 50-50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Institute of Forest Siberian Branch of Russian Academy of Sciences, 50-28 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Budker Institute of Nuclear Physics of the Siberian Branch of the Russian Academy of Sciences, 11 Lavrentyev Ave., Novosibirsk, 630090, Russian Federation

Доп.точки доступа:
Bolsunovsky, A.; Dementyev, D.; Trofimova, E.; Iniatkina, E.; Kladko, Y.; Petrichenkov, M.

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Assessment of the Quality of Bottom Sediments in the Middle Reaches of the Yenisei River by Allium test / T. A. Zotina [et al.] // Contemp. Probl. Ecol. - 2019. - Vol. 12, Is. 3. - P265-274, DOI 10.1134/S1995425519030120. - Cited References:35 . - ISSN 1995-4255. - ISSN 1995-4263

РУБ Ecology
Рубрики:
PLANT ELODEA-CANADENSIS
ANTHROPOGENIC CONTAMINATION
Кл.слова (ненормированные):
ana-telophase -- genotoxicity -- root length -- artificial sediments -- mitotic -- index -- toxicity
Аннотация: The harmful potential of bulk bottom sediments of the Yenisei River has been assessed by the Allium test. Sediment samples have been taken in ten sites on a plot of about 100 km in length in the middle reaches of the Yenisei River affected by urban activity of the city of Krasnoyarsk and industrial plants. The samples of sediments differ considerably in the content of potentially toxic substances (heavy metals, artificial radionuclides, and organic pollutants) and nutrients and in physical properties. We use the mean length of onion root as an indicator of general toxicity, the mitotic index as an indicator of cytotoxicity, and the percentage of anatelophase cells in the apical root meristem with abnormal chromosomes as an indicator of genotoxicity. Both the stimulation and inhibition of indicator endpoints are revealed in sediment samples of the Yenisei River relative to the control (artificial sediments). An increased genotoxicity is recorded in the sample of sediments contaminated by a mixture of organic and chemical toxicants. A significant positive correlation between the percentage of abnormal cells and the content of copper and petrochemicals in the samples is revealed. No extremely strong reactions of onion endpoints to the quality of sediment samples are revealed, which is probably related to relatively low contamination of the sediments studied. This is in agreement with the data of chemical analyses. Based on the testing results, we can conclude that the endpoints of the Allium test are sensitive enough to the quality of bottom sediments of the Yenisei River and can be used for their biotesting.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Fed Res Ctr, Inst Biophys,Krasnoyarsk Sci Ctr, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Zotina, T. A.; Trofimova, E. A.; Alexandrova, Yu. V.; Anishchenko, O. V.

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