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 Найдено в других БД:Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН (2)
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Общее количество найденных документов : 9
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1.


   
    Reusable biochemical diagnosis systems based on nanodiamonds / V. S. Bondar [et al.] // Doklady Biochemistry and Biophysics. - 2013. - Vol. 448, Is. 1. - P55-58, DOI 10.1134/S160767291301016X . - ISSN 1607-6729
Кл.слова (ненормированные):
cholesterol -- diamond -- nanoparticle -- article -- bioassay -- blood -- chemical model -- chemistry -- glucose blood level -- human -- methodology -- Biological Assay -- Blood Glucose -- Cholesterol -- Diamond -- Humans -- Models, Chemical -- Nanoparticles

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, Svobodnyi pr. 79, Krasnoyarsk, 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bondar, V.S.; Ronzhin, N.O.; Mamaeva, E.S.; Baron, A.V.; Gitelson, J.I.

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2.


   
    Interconnection between the spectral brightness of tomato plants grown under artificial lighting and their physiological parameters and biological productivity [Text] / A. F. Sid'Ko, A. V. Vasil'Ev, F. Y. Sid'Ko // Earth Observ. Remote Sens. - 1999. - Vol. 15, Is. 5. - P777-790. - Cited References: 15 . - 14. - ISSN 1024-5251
РУБ Geography, Physical + Geosciences, Multidisciplinary + Remote Sensing + Imaging Science & Photographic Technology

Аннотация: Optical remote methods were used for experimental determination of values of the spectral brightness coefficients (SBC) of tomato plants, grown under artificial lighting, throughout the growth period. Based on simple criteria of the ratios of the SEC it was demonstrated that it is possible to determine the physiological stress state and biological productivity of plants. The possibility of early diagnosis of the productivity of tomato plants was demonstrated.

Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sid'Ko, A.F.; Vasil'Ev, A.V.; Sid'Ko, F.Y.

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3.


   
    Calcium-regulated photoproteins of marine coelenterates [Text] / E. S. Vysotski, S. V. Markova, L. A. Frank // Mol. Biol. - 2006. - Vol. 40, Is. 3. - P355-367, DOI 10.1134/S0026893306030022. - Cited References: 99 . - ISSN 0026-8933
РУБ Biochemistry & Molecular Biology
Рубрики:
BIOLUMINOMETRIC HYBRIDIZATION ASSAYS
   HYDROID OBELIA-GENICULATA

   GREEN-FLUORESCENT PROTEIN

   POLYMERASE-CHAIN-REACTION

   BIOLUMINESCENT IMMUNOASSAY

   RECOMBINANT AEQUORIN

   CRYSTAL-STRUCTURE

   BIOTINYLATED AEQUORIN

   ANGSTROM RESOLUTION

   CA2+-REGULATED PHOTOPROTEINS

Кл.слова (ненормированные):
bioluminescence -- obelin -- aequorin -- intracellular calcium -- molecular diagnosis
Аннотация: Calcium-regulated photoproteins are bioluminescent proteins that are responsible for the luminescence of marine coelenterates. A photoprotein molecule is a stable enzyme-substrate complex consisting of a single polypeptide chain and an oxygen-preactivated substrate, 2-hydroperoxcoelenterazine, which is tightly but noncovalently bound with the protein. Bioluminescence is triggered by Ca2+ and results from decarboxylation of the substrate bound with the protein. This review considers the current information about the structure of photoproteins, the mechanism of the bioluminescent reaction, the function of particular amino acid residues of the active center in catalysis and the formation of the emitter, and the use of photoproteins in bioluminescent microanalysis.

Держатели документа:
Russian Acad Sci, Siberian Div, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Vysotski, E.S.; Markova, S.V.; Frank, L.A.

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4.


   
    PROSPECTS FOR APPLICATION OF BIOLUMINESCENCE METHOD IN MEDICINE [Текст] / I. I. GITELZON, T. P. SANDALOVA // VESTNIK AKADEMII MEDITSINSKIKH NAUK SSSR. - 1990. - Is. 9. - С. 31-35. - Cited References: 41 . - ISSN 0002-3027
РУБ Medicine, General & Internal
Рубрики:
AMINO-ACID SEQUENCE
   NUCLEOTIDE-SEQUENCE

   VIBRIO-HARVEYI

   BACTERIAL LUCIFERASE

   FIREFLY LUCIFERASE

   SUBUNIT

   CELLS

   GENE

   PHOTOPROTEINS

   EXPRESSION

Аннотация: Major advances in the development and application of the bioluminescent analysis to detect certain biologically active substances are discussed. The main merit of the method lies in its high sensitivity and specificity along with its simplicity and rapid performance. The available methodologies allow for detection of substances of varying nature: Ca2+, ATP, FMN, NAD(P), long-chain aldehydes, ATP- and NAD(P)-dependent enzymes and their substrates, many xenobiotics and antibiotics, and mutagens. The bioluminescence methodologies may be widely applied in clinical laboratory diagnosis.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
GITELZON, I.I.; SANDALOVA, T.P.

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5.


   
    RNA aptamer against autoantibodies associated with multiple sclerosis and bioluminescent detection probe on its basis [] / M. A. Vorobjeva [et al.] // Anal. Chem. - 2014. - Vol. 86, Is. 5. - P2590-2594, DOI 10.1021/ac4037894 . - ISSN 0003-2700
Кл.слова (ненормированные):
Autoantibodies -- Detection probes -- Developed model -- High affinity -- Laboratory test -- Microplate assay -- Multiple sclerosis -- Myelin basic protein -- Antibodies -- RNA -- Bioluminescence
Аннотация: Nowadays, there are no specific laboratory tests for establishing the diagnosis of multiple sclerosis (MS). The presence of proteolytic autoantibodies against myelin basic protein is now considered as a characteristic feature of MS. New 2?-F-containing RNA aptamer of high affinity and specificity to these antibodies was selected. Covalent conjugate of this aptamer and Ca 2+-regulated photoprotein obelin was obtained for the first time and applied as a label in bioluminescent microplate assay to detect target antibodies. The developed model solid-phase microassay is simple, fast, and highly sensitive. © 2014 American Chemical Society.

Scopus
Держатели документа:
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk 630090, Russian Federation
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, Krasnoyarsk 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Vorobjeva, M.A.; Krasitskaya, V.V.; Fokina, A.A.; Timoshenko, V.V.; Nevinsky, G.A.; Venyaminova, A.G.; Frank, L.A.

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6.


   
    Bioluminescent SNP genotyping technique: Development and application for detection of melanocortin 1 receptor gene polymorphisms / E. E. Bashmakova [et al.] // Talanta. - 2018. - Vol. 189. - P111-115, DOI 10.1016/j.talanta.2018.06.057 . - ISSN 0039-9140
Кл.слова (ненормированные):
Ca2+-regulated photoprotein obelin -- Genotyping -- Melanocortin 1 receptor gene -- Single nucleotide polymorphisms (SNP) -- Bioluminescence -- Clinical research -- Curricula -- Diagnosis -- Genes -- Oncology -- Biomedical research -- Clinical characteristics -- Development and applications -- Genotyping -- Healthy individuals -- Photoproteins -- Receptor genes -- Single-nucleotide polymorphisms -- Dermatology
Аннотация: SNP genotyping based on the reaction of specific primer extension with the following bioluminescent detection of its products was shown to be potentially applicable for biomedical exploration. The paper describes its elaboration and first application in extensive biomedical research concerning MC1R gene variants’ frequency and associations with clinical characteristics in melanoma patients of Eastern Siberia (Krasnoyarsk region, Russia). Polymorphisms rs 1805007 (R151C), rs 1805008 (R160W), and rs 1805009 (D294H) were detected in 174 DNA samples from patients with histologically proved diagnosis of cutaneous melanoma and in 200 samples from healthy individuals. All the results on bioluminescent SNP genotyping were confirmed by Sanger sequencing. Some features characteristic of the population were found, i.e. melanoma is mostly associated with R160W or R151C while variant D294H is extremely rare; simultaneous carriage of any two investigated variants is also strongly associated with melanoma; R151C is associated with ulceration and consequently the disease course is more aggressive, etc. The design of the technique allows fast evaluation of any known diagnostically important SNP frequencies and associations across population. © 2018 Elsevier B.V.

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Держатели документа:
Siberian Federal University, Svobodny pr. 79, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Akademgorodok 50/50, Krasnoyarsk, Russian Federation
Blokhin Cancer Research Center, Moscow, Russian Academy of Medical Sciences, Kashirskoye Shosse 24, Moscow, Russian Federation
Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk Lavrentiev Avenue 8, Novosibirsk, Russian Federation
State Medical University named after V.F. Voyno-Yasenetsky, Partizana Zheleznyaka St. 1, Krasnoyarsk, Russian Federation
Regional Clinical Oncology Center named after A.I. Kryzhanovsky, 1 Smolenskaya Str.16, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Bashmakova, E. E.; Krasitskaya, V. V.; Bondar, A. A.; Eremina, E. N.; Slepov, E. V.; Zukov, R. A.; Frank, L. A.

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7.


   
    The Ca2+-Regulated Photoprotein Obelin as a Tool for SELEX Monitoring and DNA Aptamer Affinity Evaluation / V. V. Krasitskaya, N. S. Goncharova, V. V. Biriukov [et al.] // Photochem. Photobiol. - 2020, DOI 10.1111/php.13274. - Cited References:25. - This work has been supported by the Russian Foundation for Basic Research (RFBR) under the grant no 18-38-00531. . - Article in press. - ISSN 0031-8655. - ISSN 1751-1097
РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
CARDIAC TROPONIN-I
   BIOLUMINESCENCE

   IMMUNOASSAY

   APTASENSOR

   DIAGNOSIS

Аннотация: Bioluminescent solid-phase analysis was proposed to monitor the selection process and to determine binding characteristics of the aptamer-target complexes during design and development of the specific aptamers. The assay involves Ca2+-regulated photoprotein obelin as a simple, sensitive and fast reporter. Applicability and the prospects of the approach were exemplified by identification of DNA aptamers to cardiac troponin I, a highly specific early biomarker for acute myocardial infarction. Two structurally different aptamers specific to various epitopes of troponin I were obtained and then tested in a model bioluminescent assay.

WOS
Держатели документа:
Fed Res Ctr KSC SB RAS, Inst Biophys SB RAS, Krasnoyarsk, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.
Inst Chem Biol & Fundamental Med SB RAS, Novosibirsk, Russia.
Fed Res Ctr KSC SB RAS, Kirensky Inst Phys, Krasnoyarsk, Russia.

Доп.точки доступа:
Krasitskaya, Vasilisa V.; Goncharova, Natalia S.; Biriukov, Vladislav V.; Bashmakova, Eugenia E.; Kabilov, Marsel R.; Baykov, Ivan K.; Sokolov, Aleksey E.; Frank, Ludmila A.; Russian Foundation for Basic Research (RFBR)Russian Foundation for Basic Research (RFBR) [18-38-00531]

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8.


   
    Highly-sensitive graphene field effect transistor biosensor using PNA and DNA probes for RNA detection / M. Tian, M. Qiao, C. Shen [et al.] // Appl Surf Sci. - 2020. - Vol. 527. - Ст. 146839, DOI 10.1016/j.apsusc.2020.146839 . - ISSN 0169-4332
Кл.слова (ненормированные):
Graphene field effect transistor -- PNA probe -- RNA detection -- Biosensors -- Clinical research -- Diagnosis -- DNA -- Field effect transistors -- Graphene -- RNA -- Biomedical research -- Clinical diagnosis -- Electrical response -- Graphene field-effect transistors -- Limit of detection -- Quantitative detection -- Three orders of magnitude -- Ultrasensitive detection -- Graphene transistors
Аннотация: DNA probe-based biosensors have been widely developed for detecting a range of analytes. However, the DNA probe-based sensors suffer from many problems, such as long hybridization time, background electrical noise, and relatively poor specificity. In this paper, we report the ultrasensitive detection for RNA by graphene field effect transistor (G-FET) biosensor using PNA and DNA probes. The limit of detection (LOD) of the PNA probe-modified G-FET sensor is down to 0.1 aM, which is three orders of magnitude lower than that of DNA probe-modified G-FET sensor. We demonstrate that both PNA and DNA probe-modified G-FET have great potential in quantitative detection of RNA. A good linear electrical response to RNA concentrations is obtained in a broad range from 0.1 aM to 1 pM for PNA probe-modified G-FET and from 100 aM to 1 pM for DNA probe-modified G-FET, respectively. The PNA probe-modified G-FET sensors significantly reduce the detection time compared to DNA probe-modified G-FET sensors. Moreover, the electrical response of PNA probe-modified G-FET biosensor to non-complementary RNA is negligible, showing high specificity for RNA detection. What's more, the G-FET sensor was also used to detect RNA in human serum, making it a promising way for future detection of RNA in biomedical research and early clinical diagnosis. © 2020 Elsevier B.V.

Scopus
Держатели документа:
Shandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, 253023, China
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Tian, M.; Qiao, M.; Shen, C.; Meng, F.; Frank, L. A.; Krasitskaya, V. V.; Wang, T.; Zhang, X.; Song, R.; Li, Y.; Liu, J.; Xu, S.; Wang, J.

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9.


   
    Highly-sensitive graphene field effect transistor biosensor using PNA and DNA probes for RNA detection / M. Tian, M. Qiao, C. C. Shen [et al.] // Appl. Surf. Sci. - 2020. - Vol. 527. - Ст. 146839, DOI 10.1016/j.apsusc.2020.146839. - Cited References:62. - We are grateful for financial support from National Natural Science Foundation of China (11604040, 61671107), Taishan Scholars Program of Shandong Province (tsqn201812104), Natural Science Foundation of Shandong Province (ZR2019PC026) and Qingchuang Science and Technology Plan of Shandong Province (2019KJJ017). . - ISSN 0169-4332. - ISSN 1873-5584
РУБ Chemistry, Physical + Materials Science, Coatings & Films + Physics,
Рубрики:
PEPTIDE NUCLEIC-ACID
   LABEL-FREE DETECTION

   SELECTIVE RECOGNITION

Кл.слова (ненормированные):
Graphene field effect transistor -- PNA probe -- RNA detection
Аннотация: DNA probe-based biosensors have been widely developed for detecting a range of analytes. However, the DNA probe-based sensors suffer from many problems, such as long hybridization time, background electrical noise, and relatively poor specificity. In this paper, we report the ultrasensitive detection for RNA by graphene field effect transistor (G-FET) biosensor using PNA and DNA probes. The limit of detection (LOD) of the PNA probe modified G-FET sensor is down to 0.1 aM, which is three orders of magnitude lower than that of DNA probe modified G-FET sensor. We demonstrate that both PNA and DNA probe-modified G-FET have great potential in quantitative detection of RNA. A good linear electrical response to RNA concentrations is obtained in a broad range from 0.1 aM to 1 pM for PNA probe-modified G-FET and from 100 aM to 1 pM for DNA probe-modified GFET, respectively. The PNA probe-modified G-FET sensors significantly reduce the detection time compared to DNA probe-modified G-FET sensors. Moreover, the electrical response of PNA probe-modified G-FET biosensor to non-complementary RNA is negligible, showing high specificity for RNA detection. What's more, the G-FET sensor was also used to detect RNA in human serum, making it a promising way for future detection of RNA in biomedical research and early clinical diagnosis.

WOS
Держатели документа:
Dezhou Univ, Inst Biophys, Shandong Key Lab Biophys, Dezhou 253023, Peoples R China.
Krasnoyarsk Sci Ctr SB RAS, Inst Biophys SB RAS, Fed Res Ctr, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Tian, Meng; Qiao, Mei; Shen, Congcong; Meng, Fanlu; Frank, Ludmila A.; Krasitskaya, Vasilisa V.; Wang, Tiejun; Zhang, Xiumei; Song, Ruihong; Li, Yingxian; Liu, Jianjian; Xu, Shicai; Wang, Jihua; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [11604040, 61671107]; Taishan Scholars Program of Shandong Province [tsqn201812104]; Natural Science Foundation of Shandong ProvinceNatural Science Foundation of Shandong Province [ZR2019PC026]; Qingchuang Science and Technology Plan of Shandong Province [2019KJJ017]

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