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Physiological-biochemical properties and the ability to synthesize polyhydroxyalkanoates of the glucose-utilizing strain of the hydrogen bacterium Ralstonia eutropha B8562 / T. G. Volova [et al.] // Applied Microbiology and Biotechnology. - 2006. - Vol. 73, Is. 3. - P429-433, DOI 10.1007/s00253-006-0460-0 . - ISSN 0175-7598
Кл.слова (ненормированные):
Biochemistry -- Carbon -- Genetic engineering -- Glucose -- Hydrogen -- Polyesters -- Cultural properties -- Glucose-utilizing mutant strain -- Nitrogen deficiency -- Polyhydroxyalkanoate (PHA) -- Bacteria -- 3 hydroxybutyric acid -- carbon dioxide -- fructose -- glucose -- hydrogen -- monomer -- polyhydroxyalkanoic acid -- sugar -- bacterium -- carbon -- comparative study -- energy -- ester -- experimental study -- glucose -- hydrogen -- physicochemical property -- article -- bacterial strain -- bacterium culture -- bacterium mutant -- biomass -- chemical analysis -- Cupriavidus necator -- energy consumption -- genetic analysis -- nonhuman -- synthesis -- Bacteria (microorganisms) -- Cupriavidus necator
Аннотация: Physiological-biochemical, genetic, and cultural properties of the glucose-utilizing mutant strain Ralstonia eutropha B8562 have been compared with those of its parent strain R. eutropha B5786. It has been shown that growth characteristics of the strain cultured on glucose as the sole carbon and energy source are comparable with those of the parent strain. Strain B8562 is characterized by high polyhydroxyalkanoate (PHA) yields on different carbon sources (CO2, fructose, and glucose). PHA accumulation in the strain batch cultured on glucose under nitrogen deficiency reaches 90 %. The major monomer in the PHA is ?-hydroxybutyric acid (more than 99 mol %); the identified minor components are ?-hydroxyvaleric acid (0.25-0.72 mol %) and ?-hydroxyhexanoic acid (0.08-1.5 mol %). The strain is a promising PHA producer on available sugar-containing media with glucose. В© Springer-Verlag 2006.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russian Federation
Krasnoyarsk State University, Svobodny av. 79, 660041 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Trusova, M.Y.; Kalacheva, G.S.; Kozhevnicov, I.V.

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Physiological-biochemical properties and the ability to synthesize polyhydroxyalkanoates of the glucose-utilizing strain of the hydrogen bacterium Ralstonia eutropha B8562. / T. G. Volova [et al.] // Applied microbiology and biotechnology. - 2006. - Vol. 73, Is. 2. - P429-433, DOI 10.1007/s00253-006-0460-0 . - ISSN 0175-7598
Кл.слова (ненормированные):
beta hydroxyvaleric acid -- beta-hydroxyvaleric acid -- carbon dioxide -- ester -- fatty acid -- fructose -- glucose -- hexanoic acid derivative -- hydrogen -- hydroxyacid -- oxygen -- polymer -- ribosome RNA -- valeric acid derivative -- article -- biotechnology -- chemistry -- metabolism -- methodology -- Wautersia eutropha -- Biotechnology -- Carbon Dioxide -- Cupriavidus necator -- Esters -- Fatty Acids -- Fructose -- Glucose -- Hexanoic Acids -- Hydrogen -- Hydroxy Acids -- Oxygen -- Pentanoic Acids -- Polymers -- RNA, Ribosomal
Аннотация: Physiological-biochemical, genetic, and cultural properties of the glucose-utilizing mutant strain Ralstonia eutropha B8562 have been compared with those of its parent strain R. eutropha B5786. It has been shown that growth characteristics of the strain cultured on glucose as the sole carbon and energy source are comparable with those of the parent strain. Strain B8562 is characterized by high polyhydroxyalkanoate (PHA) yields on different carbon sources (CO(2), fructose, and glucose). PHA accumulation in the strain batch cultured on glucose under nitrogen deficiency reaches 90 %. The major monomer in the PHA is beta-hydroxybutyric acid (more than 99 mol %); the identified minor components are beta-hydroxyvaleric acid (0.25-0.72 mol %) and beta-hydroxyhexanoic acid (0.08-1.5 mol %). The strain is a promising PHA producer on available sugar-containing media with glucose.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Trusova, M.Y.; Kalacheva, G.S.; Kozhevnicov, I.V.

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Physiological and biochemical characteristics and capacity for polyhydroxyalkanoates synthesis in a glucose-utilizing strain of hydrogen-oxidizing bacteria, Ralstonia eutropha B8562 / T. G. Volova [и др.] // Mikrobiologiya. - 2005. - Vol. 74, Is. 6. - С. 788-794 . - ISSN 0026-3656
Кл.слова (ненормированные):
Glucose-utilizing strain -- Hydrogen-oxidizing bacteria -- Polyhydroxyalkanoates -- Synthesis -- Bacteria (microorganisms) -- Cupriavidus necator -- bacterial RNA -- glucose -- hydrogen -- hydroxybutyric acid -- polymer -- RNA 16S -- article -- culture medium -- genetics -- growth, development and aging -- metabolism -- mutation -- oxidation reduction reaction -- physiology -- Wautersia eutropha -- Culture Media -- Cupriavidus necator -- Glucose -- Hydrogen -- Hydroxybutyrates -- Mutation -- Oxidation-Reduction -- Polymers -- RNA, Bacterial -- RNA, Ribosomal, 16S
Аннотация: The physiological, biochemical, genetic, and cultural characteristics of the glucose-utilizing mutant strain Ralstonia eutropha B8562 were investigated in comparison with the parent strain R. eutropha B5786. The morphological, cultural, and biochemical characteristics of strain R. eutropha B8562 were similar to those of strain R. eutropha B5786. Genetic analysis revealed differences between the 16S rRNA gene sequences of these strains. The growth characteristics of the mutant using glucose as the sole carbon and energy source were comparable with those of the parent strain grown on fructose. Strain B8562 was characterized by high yields of polyhydroxyalkanoate (PHA) from different carbon sources (CO 2, fructose, and glucose). In batch culture with glucose under nitrogen limitation, PHA accumulation reached 90% of dry weight. In PHA, ?-hydroxybutyrate was predominant (over 99 mol %); ?-hydroxyvalerate (0.25-0.72 mol %) and ?-hydroxyhexanoate (0.008-1.5 mol %) were present as minor components. The strain has prospects as a PHA producer on glucose-containing media.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Krasnoyarsk State University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kozhevnikov, I.V.; Dolgopolova, Yu.B.; Trusova, M.Yu.; Kalacheva, G.S.; Aref'eva, Yu.V.

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Physiological and biochemical characteristics and capacity for polyhydroxyalkanoates synthesis in a glucose-utilizing strain of hydrogen-oxidizing bacteria, Ralstonia eutropha B8562 / T. G. Volova [et al.] // Microbiology. - 2005. - Vol. 74, Is. 6. - P684-689, DOI 10.1007/s11021-005-0124-6 . - ISSN 0026-2617
Кл.слова (ненормированные):
Glucose-utilizing strain -- Hydrogen-oxidizing bacteria -- Polyhydroxyalkanoates -- Synthesis -- Bacteria (microorganisms) -- Cupriavidus necator
Аннотация: The physiological, biochemical, genetic, and cultural characteristics of the glucose-utilizing mutant strain Ralstonia eutropha B8562 were investigated in comparison with the parent strain R. eutropha B5786. The morphological, cultural, and biochemical characteristics of strain R. eutropha B8562 were similar to those of strain R. eutropha B5786. Genetic analysis revealed differences between the 16S rRNA gene sequences of these strains. The growth characteristics of the mutant using glucose as the sole carbon and energy source were comparable with those of the parent strain grown on fructose. Strain B8562 was characterized by high yields of polyhydroxyalkanoate (PHA) from different carbon sources (CO2, fructose, and glucose). In batch culture with glucose under nitrogen limitation, PHA accumulation reached 90% of dry weight. In PHA, ?-hydroxybutyrate was predominant (over 99 mol %); ?-hydroxyvalerate (0.25-0.72 mol %) and ?-hydroxyhexanoate (0.008-1.5 mol %) were present as minor components. The strain has prospects as a PHA producer on glucose-containing media. В© 2005 Pleiades Publishing, Inc.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Krasnoyarsk State University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kozhevnikov, I.V.; Dolgopolova, Yu.B.; Trusova, M.Yu.; Kalacheva, G.S.; Aref'eva, Yu.V.

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Problem of the energy relations between bioluminescence and respiration of luminous bacteria / I. I. Gitel'zon, R. I. Chumakova, A. M. Fish // Biophysics. - 1965. - Vol. 10, Is. 1. - P108-113 . - ISSN 0006-3509
Аннотация: 1. (1) The increase in the intensity of the luminescence of bacteria observed on addition of glucose follows a curve with a well-marked maximum after which attenuation sets in. 2. (2) For the relations studied by us between the amount of glucose and the amount of bacterial suspension (1 ml 0В·1 m solution of glucose per ml suspension) the time of attaining the maximum intensity of luminescence and the ratio of the maximum intensity of emission to its level before addition of glucose do not depend on the concentration of bacteria in the suspension. 3. (3) The highest uptake of glucose by bacteria occurs in the period from addition of glucose to the moment of maximum rise in luminescence. Change in the intensity of luminescence after addition of glucose follows a curve similar to that of glucose uptake. 4. (4) We have determined the radiant flux and all the radiant energy released by bacteria in the period from addition of glucose to the moment of attaining the maximum intensity. One bacterial cell on average emits 100 quanta/sec. 5. (5) Bioluminescence accounts for 10-6 parts of the entire energy released on oxidation of glucose. В© 1966.

Scopus
Держатели документа:
Institute of Physics, the Siberian Department, the U.S.S.R. Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitel'zon, I.I.; Chumakova, R.I.; Fish, A.M.

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Reusable biochemical diagnosis systems based on nanodiamonds / V. S. Bondar [et al.] // Doklady Biochemistry and Biophysics. - 2013. - Vol. 448, Is. 1. - P55-58, DOI 10.1134/S160767291301016X . - ISSN 1607-6729
Кл.слова (ненормированные):
cholesterol -- diamond -- nanoparticle -- article -- bioassay -- blood -- chemical model -- chemistry -- glucose blood level -- human -- methodology -- Biological Assay -- Blood Glucose -- Cholesterol -- Diamond -- Humans -- Models, Chemical -- Nanoparticles

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, Svobodnyi pr. 79, Krasnoyarsk, 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bondar, V.S.; Ronzhin, N.O.; Mamaeva, E.S.; Baron, A.V.; Gitelson, J.I.

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Competition of the natural and manmade biotic cycles in the closed aquatic system / T. I. Pisman, L. A. Somova // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1757-1761, DOI 10.1016/S0273-1177(03)00117-0 . - ISSN 0273-1177
Кл.слова (ненормированные):
Carbon dioxide -- Glucose -- Nitrogen compounds -- Oxygen -- Biotic cycle -- Space research -- carbon dioxide -- nitrogen -- aquatic ecosystem -- biosphere -- competition (ecology) -- endosymbiont -- animal -- article -- bacterium -- Chlorella -- comparative study -- culture medium -- food chain -- growth, development and aging -- metabolism -- microbiology -- microclimate -- Paramecium -- photosynthesis -- physiology -- symbiosis -- time -- Animals -- Bacteria -- Carbon Dioxide -- Chlorella -- Culture Media -- Ecological Systems, Closed -- Food Chain -- Nitrogen -- Paramecium -- Photosynthesis -- Symbiosis -- Time Factors -- Water Microbiology
Аннотация: This study addresses competition between the Paramecium bursaria and zoochlorella - endosymbiosis and the infusoria Paramecium caudatum in a closed aquatic system. The system is a natural model of a simple biotic cycle. P. bursaria consumes glucose and oxygen released by its zoochlorella and releases nitrogenous compounds and carbon dioxide necessary for algal photosynthesis. P. caudatum was fed on bacteria. It was shown that the infusoria P. bursaria united in one cycle with Chlorella had a higher competitive ability than P. caudatum. With any initial percentage of the infusoria in the mixed culture, the end portion of P. bursaria reached 90 - 99%, which was significantly higher than the end portion of the P. caudatum population. It is assumed that the sustenance expenditures of P. caudatum were greater than those of the endosymbiotic paramecium, i.e. the closing of the components into a biotic cycle leads to a decrease in sustenance expenditures. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, SB RAS, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pisman, T.I.; Somova, L.A.

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Heterogeneity of the populations of marine luminescent bacteria Photobacterium leiognathi under different conditions of cultivation / S. E. Medvedeva, O. A. Mogil'naya, L. Yu. Popova // Microbiology. - 2006. - Vol. 75, Is. 3. - P292-299, DOI 10.1134/S002626170603009X . - ISSN 0026-2617
Кл.слова (ненормированные):
Bioluminescence -- Luminescent bacteria -- Morphology of colonies -- Bacteria (microorganisms) -- Photobacterium leiognathi
Аннотация: Manifestation of pleiotropic effects in the isogenic variants of the luminescent bacterium Photobacterium leiognathi 54 was investigated. The decrease or increase of the expression level of bioluminescence was caused by changes in lux operon regulation. The dynamics of the bioluminescence of dark and dim variants did not differ from the dynamics of the initial luminescent variant, but dependence of the level of luminescence intensity on the exogenous autoinducer of the lux operon was revealed. The investigated variants of P. leiognathi 54 inherited fairly stable morphological characteristics, colony architectonics, level of luminescence, and activity of some enzymes; variants with reduced bioluminescence formed colonies of the S type. Stable bright variants with S-and R-type colonies appeared both in the initial strain population and in the dark variant population, but with smaller frequency. Populations of the bright variant with R-type colonies were most heterogeneous; this can be determined by the lack of glucose repression of the bioluminescence in contrast to other investigated inherited variants of P. leiognathi. В© Pleiades Publishing, Inc., 2006.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, 50, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Medvedeva, S.E.; Mogil'naya, O.A.; Popova, L.Yu.

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A NEW EXPERIMENTAL APPROACH TO THE SEARCH FOR CHEMICAL DENSITY FACTORS IN THE REGULATION OF MONOCULTURE GROWTH [Text] / A. G. DEGERMENDZHY, V. V. ADAMOVICH, V. A. ADAMOVICH // J. Gen. Microbiol. - 1993. - Vol. 139. - P2027-2031. - Cited References: 7 . - 5. - ISSN 0022-1287

РУБ Microbiology

Аннотация: In monocultures of micro-organisms, growth is controlled by feedback mechanisms involving chemical factors such as limiting substrates and inhibitory metabolic products. The role of such feedback in the growth regulation of Escherichia coli O-124 was investigated by growing cells in batch culture using a medium containing glucose and mineral salts. In various phases of growth, portions of the native culture were diluted with culture filtrate, so that although cell density decreased, the chemical composition of the growth medium was unaltered. As the diluted cultures grew, variations in growth acceleration were calculated and compared with those of native (undiluted) cultures. Towards the end of the exponential phase and in the growth deceleration phase, the specific feedback level (FBL) was between -20 and -200 (h g l-1)-1. The feedback components resulting from changes in glucose concentration were calculated using experimentally determined values of mu(max) (0.55 +/- 0.05 h-1) and K(s) (2.5 +/- 0.7 mg l-1). Only 0.1-40% of FBL could be accounted for by changes in glucose concentration, indicating the presence of additional growth regulators. The method developed may become a new tool for determination of growth-regulating cell-density factors in microbial cultures.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
DEGERMENDZHY, A.G.; ADAMOVICH, V.V.; ADAMOVICH, V.A.

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10.

A NEW EXPERIMENTAL-METHOD TO REVEAL DENSITY-DEPENDENT CHEMICAL FACTORS INVOLVED IN MICROBIAL-POPULATION GROWTH-CONTROL [Text] / A. G. DEGERMENDZHI, V. V. ADAMOVICH, V. A. ADAMOVICH // Microbiology. - 1993. - Vol. 62, Is. 3. - P308-313. - Cited References: 11 . - 6. - ISSN 0026-2617

РУБ Microbiology

Аннотация: Experiments based on an earlier developed theory with a culture of Escherichia coli 0-124 were undertaken to determine the feedback strength (FBS) in growth control. FBS is defined as the change in growth acceleration brought about by a discontinuous reduction in microbial concentration under conditions of constant chemical composition of the medium, and is an over-all measure of the regulatory influence of all chemical factors dependent on biomass concentration. FBS was determined for batch cultivation with glucose. In various growth phases the culture was diluted by its own filtrate and then the change in growth acceleration as compared to the control was measured. FBS was reliably different from zero at the end of the exponential phase and in the phase of decelerated growth and was of the order of -20 to -200 (h.g/liter)(-1). The feedback component due to glucose alone was estimated using the obtained relation between specific growth rate and glucose concentration (mu(max) = 0.55 +/- 0.05 h(-1), K-s = 2.5 +/- 0.7 mg/liter). The glucose-dependent regulatory contribution to FBS was found to be as low as 0.5 to 40%, which suggests the presence of other growth control factors. Upon accuracy improvements, this method may develop into an accepted microbiological tool for identifying major density-dependent factors involved in microbial growth control.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
DEGERMENDZHI, A.G.; ADAMOVICH, V.V.; ADAMOVICH, V.A.

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11.

Salt-dependent inhibition of light emitting of the luminescent microorganism Escherichia coli Z9051 [Текст] / A. N. Boyandin, L. Y. Popova // Biofizika. - 2001. - Vol. 46, Is. 2. - С. 251-255. - Cited References: 12 . - 5. - ISSN 0006-3029

РУБ Biophysics

Кл.слова (ненормированные):
bacterial luminescence -- recombinant plasmid -- salt concentration
Аннотация: The influence of some mineral salts on the recombinant strain Escherichia coli Z9051 was investigated. It was shown that the composition (NaCl, Na2SO4, MgCl2 and MgSO4) and concentration (5 and 10%) of the salts substantially affect the expression of genes for the luminescence system of fight-emitting bacteria cloned in the plasmid under the control of the lac-promoter. In some cases, the luminescence level of the microorganism in the presence of salts was similar to the luminescence level under catabolite repression by glucose, the more strong influence of the salts exceeding the effect of catabolite repression. The possibility of adaptation of the genetically modified microorganism to the salinity factor is discussed.

Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Popova, L.Y.

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12.

Modified nanodiamonds obtained by detonation synthesis in constructing biochemical indication systems (As exemplified by the glucose determination system) [Text] / E. S. Mamaeva [et al.] // Dokl. Biochem. Biophys. - 2011. - Vol. 439, Is. 1. - P182-184, DOI 10.1134/S1607672911040090. - Cited References: 9. - This work was partially supported by the Presidium of the Russian Academy of Sciences (program no. 21, project no. 3.6.3). . - ISSN 1607-6729

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
CARRIERS

Держатели документа:
[Mamaeva, E. S.
Puzyr, A. P.
Bondar, V. S.] Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
[Mamaeva, E. S.
Baron, A. V.
Bondar, V. S.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
[Burov, A. E.] Russian Acad Sci, Siberian Branch, Krasnoyarsk Res Ctr, SCTB Sci, Krasnoyarsk 630036, Russia
СКТБ КНЦ СО РАН
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Mamaeva, E.S.; Baron, A.V.; Puzyr, A.P.; Burov, A.E.; Bondar, V.S.

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13.

Population dynamics of an algal-bacterial cenosis in closed ecological system [Text] / T. I. Pisman, Y. V. Galayda, N. S. Loginova ; ed. YV Galayd // SPACE LIFE SCIENCES: CLOSED ECOLOGICAL SYSTEMS: EARTH AND SPACE APPLICATIONS. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2005. - Vol. 35: Workshop on Closed Ecological Systems (JUL, 2004, Paris, FRANCE), Is. 9. - P1579-1583, DOI 10.1016/j.asr.2005.03.073. - Cited References: 14 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Ecology + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences

Кл.слова (ненормированные):
microalgae-bacteria relationships -- photosynthates -- detritus -- Chlorella -- biotic cycle
Аннотация: The paper deals with microalgae-bacteria interrelationships in the "autotroph-heterotroph" aquatic biotic cycle. Explanations of why and how algal-bacterial ecosystems are formed still remain controversial. The paper presents results of experimental and theoretical investigations of the functioning of the algal-bacterial cenosis (the microalga Chlorella vulgaris and concomitant microflora). The Chlorella microbial community is dominated by representatives of the genus Pseudomonas. Experiments with non-sterile batch cultures of Chlorella on Tamiya medium showed that the biomass of microorganisms increases simultaneously with the increase in microalgal biomass. The microflora of Chlorella can grow on organic substances released by photosynthesizing Chlorella. Microorganisms can also use dying Chlorella cells, i.e. form a "producer-reducer" biocycle. To get a better insight into the cenosis-forming role of microalgae, a mathematical model of the "autotroph-heterotroph" aquatic biotic cycle has been constructed, taking into account the utilization of Chlorella photosynthates and dead cells by microorganisms and the contribution of the components to the nitrogen cycle. A theoretical study showed that the biomass of concomitant bacteria grown on glucose and detritus is larger than the biomass of bacteria utilizing only microalgal photosynthates, which agrees well with the experimental data. (c) 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.

Держатели документа:
Krasnoyarsk State Univ, Krasnoyarsk 660041, Russia
RAS, SB, Inst Biophys, Krasnoyarsk 660036, Russia
Krasnoyarsk State Med Acad, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pisman, T.I.; Galayda, Y.V.; Loginova, N.S.; Galayd, YV \ed.\

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14.

EXPERIMENTAL-MODEL OF A GAS-CLOSED AUTOTROPH HETEROTROPH ECOSYSTEM [Text] / T. I. PISMAN [et al.] // Microbiology. - 1995. - Vol. 64, Is. 5. - P560-562. - Cited References: 7 . - ISSN 0026-2617

РУБ Microbiology

Аннотация: The dynamics of components were studied in a gas-closed autotroph-heterotroph ecosystem with spatially separated components. Oxygen-producing green microscopic algae served as the autotrophic component, and carbon dioxide-producing yeasts served as the heterotrophic component. The longevity of the gas-closed autotroph-heterotroph system was two times greater than that of separately cultivated components. Making the system more closed by additing glucose (the limiting factor for heterotroph growth) prolonged the longevity of the system up to 25 days. Further system closing by complicating the heterotrophic component (comprised now of two yeast species differing in the rate of substrate consumption) further increased the longevity of the system and the biomass of both the autotrophic and heterotrophic components.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
PISMAN, T.I.; SOMOVA, L.A.; SARANGOVA, A.B.; PECHURKIN, N.S.

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15.

A glucose-utilizing strain, cupriavidus euthrophus B-10646: Growth kinetics, characterization and synthesis of multicomponent PHAs / T. Volova [et al.] // PLoS ONE. - 2014. - Vol. 9, Is. 2, DOI 10.1371/journal.pone.0087551 . - ISSN 1932-6203
Кл.слова (ненормированные):
3 hydroxybutyrate 3 hydroxyhexanoate 3 hydroxyvalerate copolymer -- 3 hydroxybutyrate 4 hydroxybutyrate 3 hydroxyvalerate copolymer -- copolymer -- gamma butyrolactone -- glucose -- hexanoic acid -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- polystyrene -- propionic acid -- unclassified drug -- valeric acid -- animal cell -- article -- bacterial growth -- bacterium culture -- cell adhesion -- cell proliferation -- crystal structure -- culture optimization -- Cupriavidus -- Cupriavidus euthrophus -- decomposition -- elasticity -- film -- glucose utilization -- kinetics -- mechanics -- melting point -- mouse -- nonhuman -- nucleotide sequence -- physical chemistry -- polymerization -- strength -- synthesis
Аннотация: This study investigates kinetic and production parameters of a glucose-utilizing bacterial strain, C. eutrophus B-10646, and its ability to synthesize PHA terpolymers. Optimization of a number of parameters of bacterial culture (cell concentration in the inoculum, physiological activity of the inoculum, determined by the initial intracellular polymer content, and glucose concentration in the culture medium during cultivation) provided cell concentrations and PHA yields reaching 110 g/L and 80%, respectively, under two-stage batch culture conditions. Addition of precursor substrates (valerate, hexanoate, propionate, ?-butyrolactone) to the culture medium enabled synthesis of PHA terpolymers, P(3HB/3HV/4HB) and P(3HB/ 3HV/3HHx), with different composition and different molar fractions of 3HB, 3HV, 4HB, and 3HHx. Different types of PHA terpolymers synthesized by C. eutrophus B-10646 were used to prepare films, whose physicochemical and physical-mechanical properties were investigated. The properties of PHA terpolymers were significantly different from those of the P3HB homopolymer: they had much lower degrees of crystallinity and lower melting points and thermal decomposition temperatures, with the difference between these temperatures remaining practically unchanged. Films prepared from all PHA terpolymers had higher mechanical strength and elasticity than P3HB films. In spite of dissimilar surface structures, all films prepared from PHA terpolymers facilitated attachment and proliferation of mouse fibroblast NIH 3T3 cells more effectively than polystyrene and the highly crystalline P3HB. Copyright: © 2014 Volova et al.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Science, Moscow, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.; Kiselev, E.; Vinogradova, O.; Nikolaeva, E.; Chistyakov, A.; Sukovatiy, A.; Shishatskaya, E.

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16.

A Glucose-Utilizing Strain, Cupriavidus euthrophus B-10646: Growth Kinetics, Characterization and Synthesis of Multicomponent PHAs [Text] / T. . Volova [et al.] // PLoS One. - 2014. - Vol. 9, Is. 2. - Ст. e87551, DOI 10.1371/journal.pone.0087551. - Cited References: 64. - This study was financially supported by Project "Biotechnologies of novel biomaterials: Innovative Biopolymers and Biomedicine Devices" (Agreement No. 11.G34.31.0013 with Amendment No. 1 of 15 February 2013) in accordance with Resolution No. 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning." The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. . - ISSN 1932-6203

РУБ Multidisciplinary Sciences
Рубрики:
RALSTONIA-EUTROPHA
   BIODEGRADABLE POLYHYDROXYALKANOATES
   AEROMONAS-HYDROPHILA
   ESCHERICHIA-COLI
   MOLECULAR-WEIGHT
   SURFACE-ENERGY
   NORTH PACIFIC
   TERPOLYESTER
   BIOSYNTHESIS
   POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE-CO-3-HYDROXYHEXANOATE)
Аннотация: This study investigates kinetic and production parameters of a glucose-utilizing bacterial strain, C. eutrophus B-10646, and its ability to synthesize PHA terpolymers. Optimization of a number of parameters of bacterial culture (cell concentration in the inoculum, physiological activity of the inoculum, determined by the initial intracellular polymer content, and glucose concentration in the culture medium during cultivation) provided cell concentrations and PHA yields reaching 110 g/L and 80%, respectively, under two-stage batch culture conditions. Addition of precursor substrates (valerate, hexanoate, propionate, c-butyrolactone) to the culture medium enabled synthesis of PHA terpolymers, P(3HB/3HV/4HB) and P(3HB/3HV/3HHx), with different composition and different molar fractions of 3HB, 3HV, 4HB, and 3HHx. Different types of PHA terpolymers synthesized by C. eutrophus B-10646 were used to prepare films, whose physicochemical and physicalmechanical properties were investigated. The properties of PHA terpolymers were significantly different from those of the P3HB homopolymer: they had much lower degrees of crystallinity and lower melting points and thermal decomposition temperatures, with the difference between these temperatures remaining practically unchanged. Films prepared from all PHA terpolymers had higher mechanical strength and elasticity than P3HB films. In spite of dissimilar surface structures, all films prepared from PHA terpolymers facilitated attachment and proliferation of mouse fibroblast NIH 3T3 cells more effectively than polystyrene and the highly crystalline P3HB.

WOS
Держатели документа:
[Volova, Tatiana
Kiselev, Evgeniy
Nikolaeva, Elena
Sukovatiy, Aleksey
Shishatskaya, Ekaterina] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia
[Volova, Tatiana
Vinogradova, Olga
Shishatskaya, Ekaterina] Siberian Fed Univ, Krasnoyarsk, Russia
[Chistyakov, Anton] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T...; Kiselev, E...; Vinogradova, O...; Nikolaeva, E...; Chistyakov, A...; Sukovatiy, A...; Shishatskaya, E...; Project "Biotechnologies of novel biomaterials: Innovative Biopolymers and Biomedicine Devices" [11.G34.31.0013]

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17.

Controlled expression of bacterial luminescence genes cloned in a multicopy recombinant plasmid [Text] / E. E. Maksimova [et al.] // Microbiology. - 1997. - Vol. 66, Is. 2. - P. 184-187. - Cited References: 17 . - ISSN 0026-2617

РУБ Microbiology
Рубрики:
GENETICALLY-MODIFIED MICROORGANISMS
BIOLUMINESCENCE
ENVIRONMENT
Кл.слова (ненормированные):
recombinant plasmid -- Escherichia coli -- luminescence -- catabolite repression
Аннотация: Luminescence and growth responses of the recombinant strain Escherichia coil Z905 (Ap(r)Lux(+)) to different concentrations of ampicillin depended on the source of carbon and energy. When glycerol was used as the substrate, the intensity of luminescence rose with the ampicillin concentration in the medium. Glucose caused catabolite repression of cell luminescence up to the late stationary phase, and ampicillin enhanced this effect. The feasibility of controlling the expression of cloned lux genes was shown.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Maksimova, E.E.; Popova, L.Y.; Kargatova, T.V.; Shpagina, V.V.

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18.

Salt-dependent inhibition of light emitting of the luminescent microorganism Escherichia coli Z9051 [Текст] / A. N. Boyandin, L. Y. Popova // Biofizika. - 2001. - Vol. 46, Is. 2. - P. 251-255. - Cited References: 12 . - ISSN 0006-3029

РУБ Biophysics

Кл.слова (ненормированные):
bacterial luminescence -- recombinant plasmid -- salt concentration
Аннотация: The influence of some mineral salts on the recombinant strain Escherichia coli Z9051 was investigated. It was shown that the composition (NaCl, Na2SO4, MgCl2 and MgSO4) and concentration (5 and 10%) of the salts substantially affect the expression of genes for the luminescence system of fight-emitting bacteria cloned in the plasmid under the control of the lac-promoter. In some cases, the luminescence level of the microorganism in the presence of salts was similar to the luminescence level under catabolite repression by glucose, the more strong influence of the salts exceeding the effect of catabolite repression. The possibility of adaptation of the genetically modified microorganism to the salinity factor is discussed.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Popova, L.Y.

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19.

Competition of the natural and manmade biotic cycles in the closed aquatic system [Text] / T. I. Pisman, L. A. Somova ; ed. M Nelson [et al.] // SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P. 1757-1761, DOI 10.1016/S0273-1177(03)00117-0. - Cited References: 10 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Ecology + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences

Аннотация: This study addresses competition between the Paramecium bursaria and zoochlorella - endosymbiosis and the infusoria Paramecium caudatum in a closed aquatic system. The system is a natural model of a simple biotic cycle. P. bursaria consumes glucose and oxygen released by its zoochlorella and releases nitrogenous compounds and carbon dioxide necessary for algal photosynthesis. P. caudatum was fed on bacteria. It was shown that the infusoria P. bursaria united in one cycle with Chlorella had a higher competitive ability than P. caudatum. With any initial percentage of the infusoria in the mixed culture, the end portion of P. bursaria reached 90 - 99%, which was significantly higher than the end portion of the P. caudatum population. It is assumed that the sustenance expenditures of P. caudatum were greater than those of the endosymbiotic paramecium, i.e. the closing of the components into a biotic cycle leads to a decrease in sustenance expenditures. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
SB RAS, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pisman, T.I.; Somova, L.A.; Nelson, M \ed.\; Pechurkin, NS \ed.\; Dempster, WF \ed.\; Somova, LA \ed.\; Somo, , LA \ed.\

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20.

Development and morphological features of biofilms formed by transgenic and wild type strains of Bacillus subtilis [Text] / O. A. Mogilnaya, T. Y. Krylova, L. Y. Popova // Microbiol. Res. - 2003. - Vol. 158, Is. 4. - P. 327-335, DOI 10.1078/0944-5013-00212. - Cited References: 17 . - ISSN 0944-5013

РУБ Microbiology
Рубрики:
ANTIBIOTIC-RESISTANCE
MICROBIAL BIOFILMS
COMMUNITY
Кл.слова (ненормированные):
Bacillus subtilis -- biofilms -- transgenic microorganisms -- electron microscopy
Аннотация: The study addressed the ability of the transgenic strain (TM) B. subtilis 2335/pBMB 105 (Km(r)Inf(+)) to form biofilms on the surface of liquid media of various compositions, inoculated with vegetative cells and spores. The morphological features of these biofilms do not differ from those of the films formed by the recipient strain (WT) B. subtilis 2335 (Km(s)). However, the TM and the natural one differ in the dynamics of biofilm formation and the cellular composition of the films. Biofilms of the TM are formed earlier, develop at a higher rate, but decompose later than the films of the WT. When the medium is inoculated with vegetative cells, sporulation in the biofilms of both strains undergoes glucose repression; no such effect is observed when the medium is inoculated with spores. The TM does not form films when the medium is inoculated with spores and supplemented with glycerin and kanamycin.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Mogilnaya, O.A.; Krylova, T.Y.; Popova, L.Y.

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