Библиотека института биофизики СО РАН

Главная

Базы данных

Труды сотрудников ИБФ СО РАН - результаты поиска

Вид поиска

Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН

Иностранные журналы библиотеки Института биофизики СО РАН

Отечественные журналы библиотеки Института биофизики СО РАН

Каталог диссертаций ИБФ СО РАН

Труды сотрудников ИБФ СО РАН

Область поиска

в найденном

Найдено в других БД:

Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН (66)

Формат представления найденных документов:
полный	информационный	краткий

Отсортировать найденные документы по:
автору	заглавию	году издания	типу документа

Поисковый запрос: (<.>K=Introduction<.>)

Общее количество найденных документов : 40
Показаны документы с 1 по 20

1-20 21-40

Effect of humic acid on absorption-release processes in the bottom sediments-Yenisei river water system as studied by dual-column ion chromatography and ?-ray spectrometry / L. G. Bondareva, O. P. Kalyakina, A. Ya. Bolsunovskii // Journal of Analytical Chemistry. - 2006. - Vol. 61, Is. 4. - P354-358, DOI 10.1134/S1061934806040101 . - ISSN 1061-9348
Кл.слова (ненормированные):
Absorption -- Chromatographic analysis -- Complexation -- Gamma ray spectrometers -- Radioisotopes -- ?-ray spectrometry -- Absorption-release processes -- Dual-column ion chromatography -- Humic acid -- Organic acids
Аннотация: The effect of humic acid on absorption-release processes in the bottom sediments-Yenisei river water system was studied by dual-column ion chromatography and ?-ray spectrometry. With the use of ion chromatography, it was found that processes related to the absorption of SO 42- and Cl- anions by a solid phase with the release of NO 3- , PO 43- , and F- to a liquid phase competed in the test systems as the concentration of water-soluble organic carbon (WSOC) was increased. Only the test anions were released in the systems without the introduction of an additional amount of WSOC as humic acid. With the use of ?-ray spectrometry, it was found that the release of 60Co, 152Eu, and 241Am radionuclides to the liquid phase in the systems with added humic acid began much earlier than in the system without the addition of humic acid. In this case, the amount of released radionuclides was greater than the amount of radioisotopes released in the system without the addition of humic acid: ?25% 241Am, ?3% 152Eu, and ?0.8% 60Co in the system with added humic acid or 0.8% 152Eu and <0.1% 60Co in the system without the addition of humic acid. The 241Am radionuclide was not determined in the system without the addition of humic acid. An increase in the concentration of WSOC in the experimental system of bottom sediments-Yenisei river water initiated the release of 60Co, 152Eu, and 241Am anthropogenic radionuclides from bottom sediments because of the formation of soluble complexes capable of migration. An increase in the concentration of WSOC had almost no effect on the release of 40K and 137Cs radionuclides. В© Pleiades Publishing, Inc., 2006.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Faculty of Chemistry, Krasnoyarsk State University, Svobodnyi pr. 79, Krasnoyarsk, 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bondareva, L.G.; Kalyakina, O.P.; Bolsunovskii, A.Ya.

Найти похожие

Impaired growth of plants cultivated in a closed system: Possible reasons / J. I. Gitelson [et al.] // Advances in Space Research. - 1997. - Vol. 20, Is. 10. - P1927-1930 . - ISSN 0273-1177
Кл.слова (ненормированные):
air conditioning -- air pollutant -- article -- culture medium -- growth, development and aging -- human -- hydroponics -- methodology -- microbiology -- microclimate -- plant -- sewage -- wheat -- Air Conditioning -- Air Pollutants -- Culture Media -- Ecological Systems, Closed -- Environment, Controlled -- Humans -- Hydroponics -- Life Support Systems -- Plants -- Triticum -- Waste Disposal, Fluid -- Water Microbiology
Аннотация: Plants in experiments on "man-higher plants" closed ecosystem (CES) have been demonstrated to have inhibited growth and reduced productivity due to three basic factors: prolonged usage of a permanent nutrient solution introduction into the nutrient medium of intra-system gray water, and closure of the system. Gray water was detrimental to plants the longer the nutrient solution was used. However, higher plant growth was mostly affected by the gaseous composition of the CES atmosphere, through accumulation of volatile substances. В© 1997 COSPAR. Published by Elsevier Science Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, J.I.; Tirranen, L.S.; Borodina, E.V.; Rygalov, V.Ye.

Найти похожие

Man-made closed ecosystems-instruments for future space exploration by man / J. I. Gitelson // SAE Technical Papers. - 1994. - 24th International Conference on Environmental Systems and 5th European Symposium on Space Environmental Control Systems (20 June 1994 through 23 June 1994, FriedrichshafenDOI 10.4271/941611
Кл.слова (ненормированные):
Human knowledge -- International researches -- Long-term effects -- Low concentrations -- Russian Academy of Sciences -- SIBERIA -- Space explorations -- Space research -- Ecosystems
Аннотация: Artificial, closed ecosystems are potentially useful tools with which to explore ecosystem interactions, allowing investigations of the biology, physiology and biophysics of complex communities. They can also be used to evaluate the long-term effects of environmental constituents that are usually present at very low concentrations. The introduction of humans into such systems extends their usefulness even further, allowing the study of the influence of the environment on people, and the effects of people on the environment. Because of the great potential of such systems in adding to human knowledge, and their considerable expense, it is appropriate that such activities be international studies. The Institute of Biophysics of the Russian Academy of Sciences (Siberian Branch) has established, The International Research Center for Closed Ecosystems (Biospherics). It is located in Central Siberia near Krasnoyarsk on the Yenissei river, and is open to all interested investigators. В© Copyright 1994 Society of Automotive Engineers, Inc.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, J.I.

Найти похожие

Population dynamics of transgenic strain Escherichia coli Z905/pPHL7 in freshwater and saline lake water microcosms with differing microbial community structures / L. Yu. Popova [et al.] // Advances in Space Research. - 2005. - Vol. 35, Is. 9 SPEC. ISS. - P1573-1578, DOI 10.1016/j.asr.2005.01.037 . - ISSN 0273-1177
Кл.слова (ненормированные):
Microcosm -- Plasmid -- Population dynamics -- Transgenic microorganism -- Bacteria -- Genes -- Microorganisms -- Saline water -- Microcosms -- Plasmid -- Population dynamics -- Transgenic microorganism -- Escherichia coli -- brine -- fresh water -- inorganic salt -- bacterial gene -- conference paper -- ecosystem -- Escherichia coli -- gene expression regulation -- genetics -- growth, development and aging -- microbiology -- Micrococcus -- penicillin resistance -- plasmid -- population dynamics -- transgenic organism -- Ampicillin Resistance -- Ecosystem -- Environmental Microbiology -- Escherichia coli -- Fresh Water -- Gene Expression Regulation, Bacterial -- Genes, Bacterial -- Micrococcus -- Organisms, Genetically Modified -- Plasmids -- Population Dynamics -- Salts -- Water Microbiology
Аннотация: Populations of Escherichia coli Z905/pPHL7, a transgenic microorganism, were heterogenic in the expression of plasmid genes when adapting to the conditions of water microcosms of various mineralization levels and structure of microbial community. This TM has formed two subpopulations (ampicillin- resistant and ampicillin-sensitive) in every microcosm. Irrespective of mineralization level of a microcosm, when E. coli Z905/pPHL7 alone was introduced, the ampicillin-resistant subpopulation prevailed, while introduction of the TM together with indigenous bacteria led to the dominance of the ampicillin-sensitive subpopulation. A high level of lux gene expression maintained longer in the freshwater microcosms than in sterile saline lake water microcosms. A horizontal gene transfer has been revealed between the jointly introduced TM and Micrococcus sp. 9/pSH1 in microcosms with the Lake Shira sterile water. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Laboratory of Controlled Heterotroph Biosynthesis, Institute of Biophysics, Russian Academy of Sciences, Akademgorodok 50/50, Krasnoyarsk 660036, Russian Federation
Siberian Branch, Institute of Computational Modeling, Russian Academy of Sciences, Akademgorodok 50/50, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Popova, L.Yu.; Kargatova, T.V.; Ganusova, E.E.; Lobova, T.I.; Boyandin, A.N.; Mogilnaya, O.A.; Pechurkin, N.S.

Найти похожие

Experimental evaluation of the processes resulting from the introduction of the transgenic microorganism Escherichia coli Z905/pPHL7 (lux+) into aquatic microcosms / T. V. Kargatova [et al.] // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1769-1774, DOI 10.1016/S0273-1177(03)00119-4 . - ISSN 0273-1177
Кл.слова (ненормированные):
Escherichia coli -- Genes -- Sensitivity analysis -- Aquatic microcosms -- Space research -- genetically modified organism -- Adaptation, Physiological -- Ampicillin Resistance -- Animals -- Chemiluminescent Measurements -- Colony Count, Microbial -- Daphnia -- Ecological Systems, Closed -- Environmental Monitoring -- Escherichia coli -- Fresh Water -- Gene Expression Regulation, Bacterial -- Organisms, Genetically Modified -- Plasmids -- Protozoa -- Time Factors -- Water Microbiology
Аннотация: The processes resulting from the introduction of the transgenic microorganism (TM) E. coli Z905/pPHL7 into aquatic microcosms have been modeled experimentally. It has been shown that the TM E. coli is able to adapt to a long co-existence with indigenous heterotrophic microflora in variously structured microcosms. In more complex microcosms the numerical dynamics of the introduced E. coli Z905/pPHL7 population is more stable. In the TM populations staying in the microcosms for a prolonged time, changes are recorded in the phenotypic expression of plasmid genes (ampicillin resistance and the luminescence level) and chromosome genes (morphological and physiological traits). However, in our study microcosms, the recombinant plasmid persisted in the TM cells for 6 years after the introduction, and as the population adapts to the conditions of the microcosms, the efficiency of the cloned gene expression in the cells is restored. In the microcosms with high microalgal counts (107 cells/ml), cells with a high threshold of sensitivity to ampicillin dominate in the population of the TM E. coli Z905/pPHL7. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, SB RAS, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kargatova, T.V.; Boyandin, A.N.; Popova, L.Yu.; Pechurkin, N.S.

Найти похожие

Population dynamics of transgenic microorganisms in the different microecosystem conditions / L. Yu. Popova [et al.] // Advances in Space Research. - 2001. - Vol. 27, Is. 9. - P1571-1579, DOI 10.1016/S0273-1177(01)00248-4 . - ISSN 0273-1177
Кл.слова (ненормированные):
Ecosystems -- Escherichia coli -- Genes -- Interferons -- Luminescence -- Vaccines -- Microcosms -- Microorganisms -- fresh water -- microorganism -- adaptation -- article -- Bacillus subtilis -- comparative study -- ecosystem -- Escherichia coli -- gene expression -- genetics -- growth, development and aging -- hazardous waste -- microbiology -- Micrococcus -- osmotic pressure -- plasmid -- population dynamics -- risk assessment -- transgenic organism -- Adaptation, Physiological -- Bacillus subtilis -- Containment of Biohazards -- Ecosystem -- Escherichia coli -- Fresh Water -- Gene Expression -- Micrococcus -- Organisms, Genetically Modified -- Osmotic Pressure -- Plasmids -- Population Dynamics -- Risk Assessment -- Water Microbiology
Аннотация: The role of key environmental factors in adaptation of spore-forming and non-spore-forming transgenic microorganisms (TM) have been studied in model ecosystems. Model TM Escherichia coli Z905 (bearing plasmid genes of bacterial luminescence Ap1Lux+) has been found to have a higher adaptation potential than TM Bacillus subtilis 2335/105 (bearing genes of human ? 2-interferon Km1Inf+), planned for employment as a living vaccine under varying environmental conditions. Effects of abiotic factors on migration of natural and recombinant plasmids between microorganisms under model ecosystem conditions has been estimated. The transgenic microorganisms with low copy number survived better under introduction conditions in the microcosms studied. This trend has been shown to be independent of the microcosm type and its complexity. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Popova, L.Yu.; Lobova, T.I.; Krylova, T.Yu.; Kargatova, T.V.; Maksimova, E.E.; Boyandin, A.N.; Pechurkin, N.S.

Найти похожие

Effect of salinity on the adaptive capacity of recombinant strains of Escherichia coli and Bacillus subtilis / A. N. Boyandin [et al.] // Microbiology. - 2000. - Vol. 69, Is. 2. - P196-199 . - ISSN 0026-2617
Кл.слова (ненормированные):
Genetically modified microorganisms -- Salinity -- Salt resistance
Аннотация: The effect of different concentrations of salts on natural and recombinant strains of Bacillus subtilis and Escherichia coli was studied. The recombinant strain of B. subtilis was found to be more osmotolerant than the wild-type strain of this bacterium, whereas the opposite situation was observed for the recombinant and wild-type strains of E. coli. Some salts exerted a bacteriostatic effect on E. coli and B. subtilis. The adaptive capacity of recombinant strains depended on the number of plasmid copies in the cells. The introduction of recombinant bacteria into model ecosystems resulted in the generation of their variants with increased osmotolerance. В© 2000 MAIK "Nauka/Interperiodica".

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Lobova, T.I.; Krylova, T.Yu.; Kargatova, T.V.; Popova, L.Yu.; Pechurkin, N.S.

Найти похожие

Population heterogeneity of plasmid-bearing and plasmid-free Bacillus subtilis strains under different environmental conditions / T. Yu. Krylova [et al.] // Microbiology. - 2000. - Vol. 69, Is. 2. - P220-224 . - ISSN 0026-2617
Кл.слова (ненормированные):
Bacillus subtilis -- Heterogeneity -- Introduction -- Microcosm -- Recombinant plasmid
Аннотация: The population heterogeneity of recombinant and plasmid-free Bacillus subtilis strains introduced into aquatic microcosms was studied. After introduction, the population of the plasmid-free strain B. subtilis 2335 in microcosms has long been represented by both vegetative cells and spores, whereas, already ten days after introduction, the population of the recombinant strain B. subtilis 2335/105 (KmI)Inf+) was represented only by spores. The number of plasmid copies in the spore isolates of the recombinant strain was the same as before introduction, but the plasmid abundance in the vegetative isolates of this strain decreased. The isolates of B. subtilis 2335/105 obtained from microcosms and the variants of this strain obtained by ten successive subcultures on M9 and 0.1?M9 media with and without kanamycin (Km) differed in the number of plasmid copies, Km resistance, and maximum biomass yield during batch cultivation. Irrespective of the presence of Km, more than 50% of the variants subcultured on M9 medium showed reduced plasmid abundance. At the same time, about 70% of the variants subcultured on 0.1 ? M9 medium with Km and 90% of the variants subcultured on the same medium without Km retained the initial number of plasmid copies. The variants subcultured on media with Km retained the initial biomass level. In more than 70% of the variants isolated from media without Km, the biomass yield increased. В© 2000 MAIK "Nauka/Interperiodica".

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Krylova, T.Yu.; Popova, L.Yu.; Pechurkin, N.S.; Kashperova, T.A.; Belyavskaya, V.A.

Найти похожие

Effect of salinity on the adaptive capacity of recombinant strains of escherichia coli and bacillus subtilis / A. N. Boyandin [и др.] // Mikrobiologiya. - 2000. - Vol. 69, Is. 2. - С. 243-247 . - ISSN 0026-3656
Кл.слова (ненормированные):
Genetically modified microorganisms -- Salinity -- Salt resistance -- sodium chloride -- adaptation -- article -- Bacillus subtilis -- electrolyte balance -- Escherichia coli -- genetic recombination -- genetics -- physiology -- Adaptation, Biological -- Bacillus subtilis -- Escherichia coli -- Recombination, Genetic -- Sodium Chloride -- Water-Electrolyte Balance
Аннотация: Effect of different concentrations of salts on natural and recombinant strains of Bacillus subtilis and Escherichia coli was studied. The recombinant strain of B. subtilis was found to be more osmotolerant than the wild-type strain of this bacterium, whereas the opposite situation was observed for the recombinant and wildtype strains of E. coli. Some salts exerted a bacteriostatic effect on E. coli and B. subtilis. The adaptive capacity of recombinant strains depended on the number of plasmid copies in the cells. The introduction of recombinant bacteria into model ecosystems resulted in the generation of their variants with increased osmotolerance.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Lobova, T.I.; Yu Krylova, T.; Kargatova, T.V.; Yu Popova, L.; Pechurkin, N.S.

Найти похожие

10.

Population heterogeneity of plasmid-bearing and plasmid-free bacillus subtilis strains under different environmental conditions / T. Yu. Krylova [и др.] // Mikrobiologiya. - 2000. - Vol. 69, Is. 2. - С. 270-275 . - ISSN 0026-3656
Кл.слова (ненормированные):
Bacillus subtilis -- Heterogeneity -- Introduction -- Microcosm -- Recombinant plasmid -- article -- Bacillus subtilis -- culture medium -- genetic recombination -- genetics -- plasmid -- Bacillus subtilis -- Culture Media -- Plasmids -- Recombination, Genetic
Аннотация: The population heterogeneity of recombinant and plasmid-free Bacillus subtilis strains introduced into aquatic microcosms was studied. After introduction, the population of the plasmid-free strain B. subtilis 2335 in microcosms has long been represented by both vegetative cells and spores, whereas, already ten days after introduction, the population of the recombinant strain B. subtilis 2335/105 (Km rInf +) was represented only by spores. The number of plasmid copies in the spore isolates of the recombinant strain was the same as before introduction, but the plasmid abundance in the vegetative isolates of this strain decreased. The isolates of B. subtilis 2335/105 obtained from microcosms and the variants of this strain obtained by ten successive subcultures on M9 and 0.1 ? M9 media with and without kanamycin (Km) differed in the number of plasmid copies, Km resistance, and maximum biomass yield during batch cultivation. Irrespective of the presence of Km, more than 50% of the variants subcultured on M9 medium showed reduced plasmid abundance. At the same time, about 70% of the variants subcultured on 0.1 ? M9 medium with Km and 90% of the variants subcultured on the same medium without Km retained the initial number of plasmid copies. The variants subcultured on media with Km retained the initial biomass level. In more than 70% of the variants isolated from media without Km, the biomass yield increased.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Krylova, T.Yu.; Popova, L.Yu.; Pechurkin, N.S.; Kashperova, T.A.; Belyavskaya, V.A.

Найти похожие

11.

Phenotypic variability of the population of a recombinant luminescent strain of escherichia coli in aqueous microcosms / T. V. Kargatova [и др.] // Mikrobiologiya. - 1997. - Vol. 66, Is. 1. - С. 101-106 . - ISSN 0026-3656
Кл.слова (ненормированные):
article -- Escherichia coli -- genetic recombination -- genetics -- luminescence -- microbiology -- penicillin resistance -- phenotype -- plasmid -- Ampicillin Resistance -- Escherichia coli -- Luminescent Measurements -- Phenotype -- Plasmids -- Recombination, Genetic -- Water Microbiology
Аннотация: The behavior of Escherichia coli Z905, carrying a recombinant plasmid pPHL7 with genes determining ampicillin resistance and bacterial luminescence, and the efficiency of expression of cloned genes were studied after introduction of the strain into model aqueous ecosystems with different trophic chain lengths. The E. coli Z905 variants isolated from ecosystems after different periods of time were found to vary in their resistance to ampicillin (from 50 to 0.05 ?g/ml) and in the intensity of bioluminescence. An increase in the concentration of the selective factor (ampicillin) or in the extent of the aqueous microcosm blooming restored the expression of the recombinant plasmid genes in some clones.

Scopus
Держатели документа:
Institute uf Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kargatova, T.V.; Maksimova, E.E.; Popova, L.Yu.; Bril'Kov, A.V.; Pechurkin, N.S.

Найти похожие

12.

Phenotypic variability of the population of a recombinant luminescent strain of escherichia coli in aqueous microcosms / T. V. Kargatova [et al.] // Microbiology. - 1997. - Vol. 66, Is. 1. - P85-90 . - ISSN 0026-2617
Аннотация: The behavior of Escherichia coli Z905, carrying a recombinant plasmid pPHL7 with genes determining ampicillin resistance and bacterial luminescence, and the efficiency of expression of cloned genes were studied after introduction of the strain into model aqueous ecosystems with different trophic chain lengths. The E. coli Z905 variants isolated from ecosystems after different periods of time were found to vary in their resistance to ampicillin (from 50 to 0.05 ?g/ml) and in the intensity of bioluminescence. An increase in the concentration of the selective factor (ampicillin) or in the extent of the aqueous microcosm blooming restored the expression of the recombinant plasmid genes in some clones. В© 1997 MAHK Hayka/Interperiodica Publishing.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kargatova, T.V.; Maksimova, E.E.; Popova, L.Yu.; Bril'kov, A.V.; Pechurkin, N.S.

Найти похожие

13.

Effect of environmental factors on the expression of the catabolite-dependent lux-operon borne by a recombinant plasmid / E. E. Maksimova [et al.] // Microbiology. - 1998. - Vol. 67, Is. 2. - P137-141 . - ISSN 0026-2617
Кл.слова (ненормированные):
Catabolite repression -- Environmental factors -- Escherichia coli -- Introduction into model ecosystems -- Lux-operon -- Recombinant plasmid -- Regulation of expression
Аннотация: Expression of the lux-genes cloned in the recombinant plasmid pPHL7 (Ap1Lux+) in Escherichia coli Z905 cells was studied in various environments, including model aquatic ecosystems. Expression of the lux-genes strongly depended on the nutritional status of the medium. In particular, the cultivation of cells in nutrient-rich medium favored the maintenance of the initial level of expression of the lux-operon, whereas nutrient limitation induced recombinant cell variants with an impaired control of the catabolite-dependent luxoperon. On the other hand, long-term laboratory cultivation of the recombinant strain in nutrient-deficient media or its long-term life in model aquatic ecosystems led to the accumulation of cells with a stringent control on the cloned lux-genes in the bacterial population. The presence of the selective factor (ampicillin) in the medium had no significant effect on the expression of the lux-operon. В© 1998 MAHK Hayka/Interperiodica Publishing.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Maksimova, E.E.; Popova, L.Yu.; Shpagina, V.V.; Belyavskaya, V.A.; Pechurkin, N.S.

Найти похожие

14.

Effect of environmental factors on the expression of the catabolite-dependent lux-operon borne by a recombinant plasmid / E. E. Maksimova [и др.] // Mikrobiologiya. - 1998. - Vol. 67, Is. 2. - С. 170-175 . - ISSN 0026-3656
Кл.слова (ненормированные):
Catabolite repression -- Environmental factors -- Escherichia coli -- Introduction into model ecosystems -- Lux-operon -- Recombinant plasmid -- Regulation of expression -- recombinant DNA -- article -- bacterial gene -- chemoluminescence -- culture medium -- Escherichia coli -- gene expression regulation -- genetics -- microbiology -- molecular cloning -- operon -- plasmid -- Chemiluminescent Measurements -- Cloning, Molecular -- Culture Media -- DNA, Recombinant -- Escherichia coli -- Gene Expression Regulation, Bacterial -- Genes, Bacterial -- Operon -- Plasmids -- Water Microbiology
Аннотация: Expression of the lux-genes cloned on the recombinant plasmid pPHL7 (Ap rLux +) in Escherichia coli Z905 cells was studied in various environments, including model aquatic ecosystems. Expression of the lux-genes strongly depended on the nutritional status of the medium. In particular, the cultivation of cells in nutrient-rich medium favored the maintenance of the initial level of expression of the lux-operon, whereas nutrient limitation induced recombinant cell variants with an impaired control of the catabolite-dependent luxoperon. On the other hand, long-term laboratory cultivation of the recombinant strain in nutrient-deficient media or its long-term life in model aquatic ecosystems led to the accumulation of cells with a stringent control on the cloned lux-genes in the bacterial population. The presence of the selective factor (ampicillin) in the medium had no significant effect on the expression of the lux-operon.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Maksimova, E.E.; Popova, L.Yu.; Shpagina, V.V.; Belyavskaya, V.A.; Pechurkin, N.S.

Найти похожие

15.

Introduction and long-term storage of recombinant luminescent Escherichia coli strain Z905 in laboratory water microecosystems / L. I. Popova [и др.] // Izvestiia Akademii nauk. Seriia biologicheskaia / Rossiiskaia akademiia nauk. - 1998. - Is. 6. - С. 670-677 . - ISSN 1026-3470
Кл.слова (ненормированные):
article -- bacterial gene -- chemoluminescence -- Escherichia coli -- gene expression -- genetic recombination -- genetics -- laboratory -- microbiology -- Chemiluminescent Measurements -- Escherichia coli -- Gene Expression -- Genes, Bacterial -- Laboratories -- Recombination, Genetic -- Water Microbiology
Аннотация: We studied preservation of recombinant Escherichia coli strain Z905 (AprLux+) in liquid microecosystems (LME) after the introduction. E. coli cells were shown to remain viable and preserve the ability to express the cloned lux genes for a long time (more than a year) in LME. The majority of the clones have reduced efficiency of the expression due to either changed regulation of the lux operon or decreased number of copies of the plasmid. These mechanisms could be realized either independently or simultaneously depending on LME conditions. We have exposed the major factors affecting the metabolic activity of the E. coli strain Z905 (AprLux+) introduced into model ecosystems and the level of expression of the cloned genes.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Popova, L.I.; Maksimova, E.E.; Kargatova, T.V.; Bril'kov, A.V.; Pechurkin, N.S.

Найти похожие

16.

Micro- and macroorganisms in life support systems (problem of coexistence) / L. A. Somova, N. S. Pechurkin, R. C. Huttenbach // Advances in Space Research. - 1996. - Vol. 18, Is. 12. - P259-263, DOI 10.1016/0273-1177(96)00120-2 . - ISSN 0273-1177
Аннотация: Every life support system has an associated microflora that is not essential to functioning of the system. At the same time, the confined space of a closed system increases the significance of the associated microflora causing closer contact between components and enhancing the intensity of exchange between them. For any life support system that is functioning normally, there exists an optimum between the effort necessary to maintain the system in a healthy state and the damage the introduction of alien microflora can cause. Copyright В© 1996 COSPAR.

Scopus
Держатели документа:
Institute of Biophysics, Krasnoyarsk, 660036, Russian Federation
Nelson Space Services Ltd., 90 London Road, London SEI 6LN, United Kingdom : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Somova, L.A.; Pechurkin, N.S.; Huttenbach, R.C.

Найти похожие

17.

Modelling of genetically engineered microorganisms introduction in closed artificial microcosms / N. S. Pechurkin [et al.] // Advances in Space Research. - 1999. - Vol. 24, Is. 3. - P335-341, DOI 10.1016/S0273-1177(99)00320-8 . - ISSN 0273-1177
Кл.слова (ненормированные):
aquatic environment -- artificial ecosystem -- ecological modeling -- genetically modified organism -- alga -- animal -- article -- bacterial count -- bacterial gene -- biological model -- biomass -- Escherichia coli -- feasibility study -- genetic engineering -- genetics -- growth, development and aging -- microbiology -- microclimate -- Photobacterium -- plasmid -- protozoon -- time -- yeast -- Algae -- Animals -- Biomass -- Colony Count, Microbial -- Ecological Systems, Closed -- Escherichia coli -- Feasibility Studies -- Genes, Bacterial -- Genetic Engineering -- Models, Biological -- Photobacterium -- Plasmids -- Protozoa -- Time Factors -- Water Microbiology -- Yeasts
Аннотация: The possibility of introducing genetically engineered microorganisms (GEM) into simple biotic cycles of laboratory water microcosms was investigated. The survival of the recombinant strain Escherichia coli Z905 (Ap(r), Lux+) in microcosms depends on the type of model ecosystems. During the absence of algae blooming in the model ecosystem, the part of plasmid-containing cells E.coli decreased fast, and the structure of the plasmid was also modified. In conditions of algae blooming (Ankistrodesmus sp.) an almost total maintenance of plasmid-containing cells was observed in E.coli population. A mathematics model of GEM's behavior in water ecosystems with different level of complexity has been formulated. Mechanisms causing the difference in luminescent exhibition of different species are discussed, and attempts are made to forecast the GEM's behavior in water ecosystems.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russian Federation
Krasnoyarsk State University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pechurkin, N.S.; Brilkov, A.V.; Ganusov, V.V.; Kargatova, T.V.; Maksimova, E.E.; Popova, L.Yu.

Найти похожие

18.

Effect of humic acid on absorption-release processes in the bottom sediments-Yenisei river water system as studied by dual-column ion chromatography and gamma-ray spectrometry [Text] / L. G. Bondareva, O. P. Kalyakina, A. Y. Bolsunovskii // J. Anal. Chem. - 2006. - Vol. 61, Is. 4. - P354-358, DOI 10.1134/S1061934806040101. - Cited References: 11 . - 5. - ISSN 1061-9348

РУБ Chemistry, Analytical

Аннотация: The effect of humic acid on absorption-release processes in the bottom sediments-Yenisei river water system was studied by dual-column ion chromatography and gamma-ray spectrometry. With the use of ion chromatography, it was found that processes related to the absorption of SO42- and Cl- anions by a solid phase with the release of NO3-, PO43-, and F- to a liquid phase competed in the test systems as the concentration of water-soluble organic carbon (WSOC) was increased. Only the test anions were released in the systems without the introduction of an additional amount of WSOC as humic acid. With the use of gamma-ray spectrometry, it was found that the release of Co-60, Eu-152, and Am-241 radionuclides to the liquid phase in the systems with added humic acid began much earlier than in the system without the addition of humic acid. In this case, the amount of released radionuclides was greater than the amount of radioisotopes released in the system without the addition of humic acid: similar to 25% Am-241, similar to 3% Eu-152, and similar to 0.8% Co-60 in the system with added humic acid or 0.8% Eu-152 and 0.1% Co-60 in the system without the addition of humic acid. The Am-241 radionuclide was not determined in the system without the addition of humic acid. An increase in the concentration of WSOC in the experimental system of bottom sediments-Yenisei river water initiated the release of Co-60, Eu-152, and Am-241 anthropogenic radionuclides from bottom sediments because of the formation of soluble complexes capable of migration. An increase in the concentration of WSOC had almost no effect on the release of K-40 and Cs-137 radionuclides.

Держатели документа:
Russian Acad Sci, Siberian Div, Inst Biophys, Krasnoyarsk 660036, Russia
Krasnoyarsk State Univ, Fac Chem, Krasnoyarsk 660041, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bondareva, L.G.; Kalyakina, O.P.; Bolsunovskii, A.Y.

Найти похожие

19.

Affine magnetic sorbents supported on coal ash microspheres for recombinant protein isolation / L. A. Frank [et al.] // Applied Biochemistry and Microbiology. - 2009. - Vol. 45, Is. 2. - P215-220, DOI 10.1134/S0003683809020173 . - ISSN 0003-6838
Кл.слова (ненормированные):
Clytia gregaria -- Obelia longissima
Аннотация: The results of the development and utilization of an affine magnetic sorbent with Ni2+ ions immobilized on coal ash microspheres are reported. The applicability of the material in the isolation of Histag proteins is demonstrated by examples of the recombinant green fluorescent protein from Clytia gregaria and the Ca2+ regulated photoprotein obelin from Obelia longissima. The specific sorption capacity of the sorbent was 2-7 mg/cm3 for medium-size proteins (20-30 kDa). The particles are suitable for chromatography with the presence of chaotropic agents and EDTA. They are easy to manipulate as isolation of a target protein takes 30-35 min. On the one hand, the elevated affinity of the sorbent to proteins rich in native histidines may result in a high degree of irreversible sorption; on the other hand, it allows isolation of such proteins without the introduction of artificial polyhistidine fragments. В© 2009 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Institute of Chemistry and Chemical Technology, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660049, Russian Federation
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Frank, L.A.; Borisova, V.V.; Vereshchagina, T.A.; Fomenko, E.V.; Anshits, A.G.; Gitelson, I.I.

Найти похожие

20.

Phenotypic variability of the population of a recombinant luminescent strain of Escherichia coli in aqueous microcosms [Text] / T. V. Kargatova [et al.] // Microbiology. - 1997. - Vol. 66, Is. 1. - P85-90. - Cited References: 12 . - ISSN 0026-2617

РУБ Microbiology
Рубрики:
GENETICALLY-MODIFIED MICROORGANISMS
Аннотация: The behavior of Escherichia coil Z905, carrying a recombinant plasmid pPHL7 with genes determining ampicillin resistance and bacterial luminescence, and the efficiency of expression of cloned genes were studied after introduction of the strain into model aqueous ecosystems with different trophic chain lengths. The E. coli Z905 variants isolated from ecosystems after different periods of time were found to vary in their resistance to ampicillin (from 50 to 0.05 mu g/ml) and in the intensity of bioluminescence. An increase in the concentration of the selective factor (ampicillin) or in the extent of the aqueous microcosm blooming restored the expression of the recombinant plasmid genes in some clones.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kargatova, T.V.; Maksimova, E.E.; Popova, L.Y.; Brilkov, A.V.; Pechurkin, N.S.

Найти похожие