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Surface modification of bioresorbable polymer scaffolds by laser treatment / V. V. Slabko [et al.] // Biophysics. - 2010. - Vol. 55, Is. 2. - P234-238, DOI 10.1134/S0006350910020120 . - ISSN 0006-3509
Кл.слова (ненормированные):
hydrophilicity -- laser irradiation -- microbial biocompatible and biodegradable polymers -- polyhydroxybuturate -- surface properties
Аннотация: The effect of laser irradiation on the properties of the surface of films prepared from a bioresorbable polymer poly(hydroxybuturate) has been studied. To determine the spectral region of the polymer optimal for the effective action of radiation on electron molecular bonds, theoretical investigations have been performed, which have shown that, for modifying the surface of PHB scaffolds, it is expedient to use a vacuum laser at a wavelength of 160 nm. Using laser irradiation at a power from 3 to 30 W, a series of films with modified surface, from roughnesses to perforations, have been obtained. The microstructure and properties of the film surface depending on the mode of irradiation have been examined, and conditions have been found under which the contact marginal angles of film wetting with water can be decreased to 50В° (compared with 76-80В° in starting products). Thus, conditions of laser treatment of PHB scaffolds have been theoretically substantiated and experimentally realized that provide a beneficial effect on the properties of the surface without destroying the structure of the material. В© 2010 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Biophysics, Russian Academy of Sciences, Siberian Division, Krasnoyarsk 660036, Russian Federation
Siberian State Technological University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Slabko, V.V.; Volova, T.G.; Krasnov, P.O.; Kuzubov, A.A.; Shishatskaya, E.I.

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Radioactive contamination of aquatic organisms of the Yenisei river in the area affected by the activity of the mining-and-chemical combine / A. Ya. Bolsunovskij, A. G. Sukovatyj // Radiatsionnaya Biologiya. Radioekologiya. - 2004. - Vol. 44, Is. 3. - С. 361-366 . - ISSN 0869-8031
Кл.слова (ненормированные):
Dosimetry -- Environmental impact -- Radioactivity -- River pollution -- Water analysis -- Aquatic organisms -- Diatoms -- Exposure dose rate -- Fontinalis antipyretica -- Phylolimnogammarus viridis -- The Enisei River -- Radioisotopes -- Animalia -- Bacillariophyta -- Bryophyta -- Fontinalis -- Fontinalis antipyretica -- fresh water -- radioisotope -- animal -- article -- chemical industry -- Crustacea -- diatom -- environmental monitoring -- industrial waste -- methodology -- mining -- plant -- radiation exposure -- radiation response -- Russian Federation -- water pollutant -- water pollution -- Animals -- Chemical Industry -- Crustacea -- Diatoms -- Dose-Response Relationship, Radiation -- Environmental Monitoring -- Fresh Water -- Industrial Waste -- Mining -- Plants -- Radioisotopes -- Siberia -- Water Pollutants, Radioactive -- Water Pollution
Аннотация: The study was done to investigate the content of manmade radionuclides in aquatic organisms of the Yenisei River near the Mining-and-Chemical Combine (MCC) and to estimate the exposure dose rates to organisms from various sources. The results of the investigation and calculations suggest that the main source of radioactive contamination of aquatic organisms is the coolant of the third MCC reactor, which is still being released into the Yenisei. Gamma-spectrometric analysis revealed 23 manmade radionuclides in the biomass of aquatic plants. The aquatic animal Phylolimnogammarus viridis and diatoms also contain manmade radionuclides. Among aquatic organisms, the highest dose rate is received by aquatic plants (up to 39 ?Gy/day). For most aquatic organisms under study, the dose received from the technogenic irradiation is an order of magnitude higher than the dose received from natural irradiation. The water moss (Fontinalis antipyretica) features the highest capacity to accumulate manmade radionuclides; hence, it accumulates the largest technogenic exposure dose among the study aquatic organisms.

Scopus
Держатели документа:
Inst. of Biophysics, Sib. Div. of RAS, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovskij, A.Ya.; Sukovatyj, A.G.

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Research of the meteorological factors influence on the fluorescence measurements of subsurface phytoplankton in blooming reservoir / O. V. Anishchenko, V. I. Komakov, M. I. Gladyshev // Doklady Akademii Nauk. - 2004. - Vol. 397, Is. 1. - С. 124-127 . - ISSN 0869-5652
Кл.слова (ненормированные):
Bacteria -- Fluorescence -- Meteorological problems -- Precipitation (meteorology) -- Reservoirs (water) -- Solar radiation -- Wind effects -- Meteorological effects -- Algae
Аннотация: The reaction of algae and cyano-bacteria photosynthetic apparatus on atmospheric sediments, incident solar radiation and wind velocity was estimated by measuring chlorophyll fluorescence using three-beam fluorescent diagnostics. As shown, wind in the spring positively affects the chlorophyll concentration but causes negative effect on phytoplankton photosynthetic activity in other seasons. Under conditions of high-intensity solar irradiation confervoid and colonial cyanobacteria, possessing gas vacuoles, have an advantage over plankton eukaryotic algae. Other response differences weren't observed.

Scopus
Держатели документа:
Inst. Biofiziki SO RAN, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Anishchenko, O.V.; Komakov, V.I.; Gladyshev, M.I.

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Light intensity and production parameters of phytocenoses cultivated on soil-like substrate under controled environment conditions / A. A. Tikhomirov [et al.] // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1775-1780, DOI 10.1016/S0273-1177(03)80020-0 . - ISSN 0273-1177
Кл.слова (ненормированные):
Bacteria -- Biology -- Crops -- Photosynthesis -- Substrates -- Light intensity -- Space research -- biosphere -- Biomass -- Carbon Dioxide -- Colony Count, Microbial -- Culture Media -- Ecological Systems, Closed -- Enterobacteriaceae -- Life Support Systems -- Light -- Photosynthesis -- Plant Transpiration -- Raphanus -- Soil Microbiology -- Triticum -- Water Microbiology
Аннотация: To increase the degree of closure of biological life support systems of a new generation, we used vermicomposting to involve inedible phytomass in the intra-system mass exchange. The resulting product was a soil-like substrate, which was quite suitable for growing plants (Manukovsky et al. 1996, 1997). However, the soil-like substrate can be regarded as a candidate for inclusion in a system only after a comprehensive examination of its physical, chemical, and other characteristics. An important criterion is the ability of the soil-like substrate to supply the necessary mineral elements to the photosynthesizing component under the chosen cultivation conditions. Thus, the purpose of this work was to study the feasibility of enhancing the production activity of wheat and radish crops by varying the intensity of photosynthetically active radiation, without decreasing the harvest index. The increase of light intensity from 920 to 1150 ?molВ·m -2В·s -1 decreased the intensity of apparent photosynthesis of the wheat crops and slightly increased the apparent photosynthesis of the radish crops The maximum total and grain productivity (kg/m 2) of the wheat crops was attained at the irradiance of 920 ?molВ·m -2В·s -1. Light intensity of 1150 ?molВ·m -2В·s -1 decreased the productivity of wheat plants and had no significant effect on the productivity of the radish crops (kg/m 2) as compared to 920 ?molВ·m 2В·s -1. The qualitative and quantitative composition of microflora of the watering solution and substrate was determined by the condition of plants, developmental phase and light intensity. By the end of wheat growth under 1150 ?molВ·m -2В·s -1 the numbers of bacteria of the coliform family and phytopathogenic bacteria in the watering solution and substrate were an order of magnitude larger than under other illumination conditions. The obtained data suggest that the cultivation of plants in a life support system on soil-like substrate from composts has a number of advantages over the cultivation on neutral substrates, which require continual replenishment of the plant nutrient solution from the system's store to complement the macro- and microelements. Yet, a number of problems arise, including those related to the controlling of the production activity of the plants by the intensity of photosynthetically active radiation. It is essential to understand why the intensity of production processes is limited at higher irradiation levels and to overcome the factors responsible for this, so that the soil-like substrate could have an even better chance in the competition for the best plant cultivation technology to be used in biological life support systems. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, RAS SB, Krasnoyarsk 660036, Russian Federation
Tomsk State University, Lenin street, 36, Tomsk 634050, Russian Federation
LGCB, Universte B. Pascal, BP206, Aubiere cedex 63174, France
ESA/ESTEC, Noordwijk, AG 2200, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Ushakova, S.A.; Gribovskaya, I.A.; Tirranen, L.S.; Manukovsky, N.S.; Zolotukhin, I.G.; Karnachuk, R.A.; Gros, J.-B.; Lasseur, Ch.

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Volatile metabolites of higher plant crops as a photosynthesizing life support system component under temperature stress at different light intensities / I. I. Gitelson [et al.] // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1781-1786, DOI 10.1016/S0273-1177(03)00121-2 . - ISSN 0273-1177
Кл.слова (ненормированные):
Composition -- Crops -- Heat resistance -- Metabolites -- Photosynthesis -- Volatile metabolites -- Space research -- biosphere -- article -- comparative study -- gas -- growth, development and aging -- heat -- indoor air pollution -- light -- metabolism -- microclimate -- photon -- photosynthesis -- physiology -- radiation exposure -- volatilization -- wheat -- Air Pollution, Indoor -- Environment, Controlled -- Gases -- Heat -- Life Support Systems -- Light -- Photons -- Photosynthesis -- Triticum -- Volatilization
Аннотация: The effect of elevated temperatures of 35 and 45В°C (at the intensities of photosynthetically active radiation 322, 690 and 1104 ?mol-m-2-s-1) on the photosynthesis, respiration, and qualitative and quantitative composition of the volatiles emitted by wheat (Triticum aestuvi L., cultivar 232) crops was investigated in growth chambers. Identification and quantification of more than 20 volatile compounds (terpenoids - ?-pinene, ?3 carene, limonene, benzene, ?-and transcaryophyllene, ?- and ?-terpinene, their derivatives, aromatic hydrocarbons, etc.) were conducted by gas chromatograph/mass spectrometry. Under light intensity of 1104 ?mol-m-2-s-1, heat resistance of photosynthesis and respiration increased at 35В°C and decreased at 45В°C. The action of elevated temperatures brought about variations in the rate and direction of the synthesis of volatile metabolites. The emission of volatile compounds was the greatest under a reduced irradiation of 322 ?mol-m-2-s-1 and the smallest under 1104 ?mol-m-2-s-1 at 35В°C. During the repair period, the contents and proportions of volatile compounds were different from their initial values, too. The degree of disruption and the following recovery of the functional state depended on the light intensity during the exposure to elevated temperatures. The investigation of the atmosphere of the growth chamber without plants has revaled the substances that were definitely technogenic in origin: tetramethylurea, dimethylsulfide, dibutylsulfide, dibutylphthalate, and a number of components of furan and silane nature. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, I.I.; Tikhomirov, A.A.; Parshina, O.V.; Ushakova, S.A.; Kalacheva, G.S.

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The levels of organization of the photosynthetic apparatus and the control of production processes in phytocenoses under artificial-light culture / A. A. Tikhomirov, G. M. Lisovskii // Russian Journal of Plant Physiology. - 2001. - Vol. 48, Is. 3. - P395-399, DOI 10.1023/A:1016682904411 . - ISSN 1021-4437
Кл.слова (ненормированные):
Artificial-light culture -- Photosynthetic apparatus -- Phytocenosis -- Productivity -- Embryophyta
Аннотация: The processes limiting the production in higher plant phytocenoses under an artificial-light culture are analyzed in relation to the multilevel organization of the photosynthetic apparatus (PA). The authors consider the feasibility of overcoming these limitations by optimizing the physical parameters of irradiation (the structure of the light spectrum, the rate, and the ratio of radiation fluxes in photosynthetically active radiation (PAR) and infrared (IR) regions) at the molecular, leaf, plant, and cenotic levels of PA organization. To illustrate this approach, the authors used a complex experiment in an artificial ecosystem to evaluate the efficiency of the light control of production processes in multispecies phytocenoses by alleviating or removing the factors that limit plant production at the various levels of PA organization. An artificial-light culture is seen as an instrument for solving several problems of theoretical and applied plant physiology and related disciplines in the future.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Lisovskii, G.M.

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Fine chloroplast structure in cucumber and pea leaves developed under red light / E. N. Zavorueva [et al.] // Russian Journal of Plant Physiology. - 2000. - Vol. 47, Is. 6. - P740-747 . - ISSN 1021-4437
Кл.слова (ненормированные):
Cucumis sativus -- Pigments -- Pisum sativum -- Red light -- Ultrastructure
Аннотация: Photosynthetic activity, the content of various photosynthetic pigments, and the chloroplast ultrastructure were examined in the leaves of cucumber (Cucumis sativus L.) and pea (Pisum sativum L.) plants of different ages grown under red light (600-700 nm, 100 W/m2). In pea leaves tolerant to red-light irradiation, chloroplast ultrastructure did not essentially change. In the first true leaves of cucumber plants susceptible to red-light irradiation, we observed a considerable increase in the number and size of plastoglobules, the appearance of chloroplasts lacking grana or containing only infrequent grana, and stromal thylakoids. In the upper leaves of 22-day-old cucumber plants, the chloroplast structure was essentially similar to that of the control chloroplasts in white light, and we therefore suppose that these plants have acclimated to red light.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zavorueva, E.N.; Ushakova, S.A.; Volkova, E.K.; Tikhomirov, A.A.; Mogil'naya, O.A.; Medvedeva, S.E.

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A comparative investigation of biodegradable polyhydroxyalkanoate films as matrices for in vitro cell cultures [Text] / E. I. Shishatskaya, T. G. Volova // J. Mater. Sci.-Mater. Med. - 2004. - Vol. 15, Is. 8. - P915-923, DOI 10.1023/B:JMSM.0000036280.98763.c1. - Cited References: 34 . - 9. - ISSN 0957-4530

РУБ Engineering, Biomedical + Materials Science, Biomaterials
Рубрики:
DEGRADATION
   POLY(3-HYDROXYBUTYRATE)
   POLYESTERS
   POLYMERS
Аннотация: The paper describes the production and investigation of flexible films made of high-purity polyhydroxyalkanoates (PHAs) - polyhydroxybutyrate [poly-(3HB)] and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate [poly(3Hl3-co-3HV)], containing 4-30 mol % hydroxyvalerate. Poly(3HB-co-3HV) films have a more porous structure than poly-(3HB) films, which are more compact, but their surface properties, such as wettability and surface and interface energies, are the same. Sterilisation of the PHA films by conventional methods (heat treatment and gamma-irradiation) did not impair their strength. Cells cultured on PHA films exhibited high levels of cell adhesion. Cell morphology, protein synthesis and DNA synthesis were estimated by extent of H-3-thymidine incorporation into the animal cell cultures of various origins (fibroblasts, endothelium cells, and isolated hepatocytes) in direct contact with PHAs. The investigation showed that this material can be used to make matrices for in vitro proliferous cells. The investigated properties of poly-(3HB) and poly(3HB-co-3HV) films proved to be fundamentally similar. (C) 2004 Kluwer Academic Publishers.

Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 60036, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.

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Specific features in the change of electrical resistivity of carbon nanocomposites based on nanodiamonds under neutron irradiation / S. K. Gordeev [et al.] // Phys. Solid State. - 2013. - Vol. 55, Is. 7. - P1480-1486, DOI 10.1134/S1063783413070147. - Cited References: 24. - This study was supported by the Ministry of Education and Science of the Russian Federation (state contract nos. 14.518.11.7028 and 16.518.11.7034) and the Russian Foundation for Basic Research (project no. 10-02-00576). . - 7. - ISSN 1063-7834

РУБ Physics, Condensed Matter
Рубрики:
NANOPARTICLES
Аннотация: The physical properties of bulk composite materials consisting of nanodiamond, pyrolytic carbon, and nanopores were investigated. Samples were irradiated in a channel of the reactor by fast neutrons (E 0.5MeV) in ampoules with helium and in an aqueous medium. The dependences of the electrical transport properties of materials with different compositions on the dose of irradiation with fast neutrons were studied. A nonmonotonic change in the electrical resistivity with an increase in the neutron fluence was revealed. Possible explanations were offered for the observed dependence of the electrical resistivity on the neutron fluence, in particular, those related to the physical processes occurring in surface states of the three-phase system of the nanocomposite.

Держатели документа:
[Gordeev, S. K.
Korchagina, S. B.] Cent Res Inst Mat, St Petersburg 191014, Russia
[Konopleva, R. F.
Chekanov, V. A.
Belyaev, S. P.
Golosovskii, I. V.] Natl Res Ctr Kurchatov Inst, Konstantinov Petersburg Nucl Phys Inst, Gatchina 188300, Leningrad Oblas, Russia
[Denisov, I. A.
Belobrov, P. I.] Siberian Fed Univ, Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
[Belyaev, S. P.] St Petersburg State Univ, St Petersburg 199034, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gordeev, S.K.; Konopleva, R.F.; Chekanov, V.A.; Korchagina, S.B.; Belyaev, S.P.; Golosovskii, I.V.; Denisov, I.A.; Belobrov, P.I.

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10.

Effect of low-level alpha-radiation on bioluminescent assay systems of various complexity [Text] / T. V. Rozhko [et al.] // Photochem. Photobiol. Sci. - 2007. - Vol. 6, Is. 1. - P67-70, DOI 10.1039/b614162p. - Cited References: 52 . - 4. - ISSN 1474-905X

РУБ Biochemistry & Molecular Biology + Biophysics + Chemistry, Physical
Рубрики:
LOW-DOSE RADIATION
   IRRADIATION
   TOXICITY
   QUINONES
   HORMESIS
   PHENOLS
Аннотация: This study addresses the effects of low-level alpha-radiation on bioluminescent assay systems of different levels of organization: in vivo and in vitro. Three bioluminescent assay systems are used: intact bacteria, lyophilized bacteria, and bioluminescent system of coupled enzyme reactions. Solutions of Am-241(NO3)(3) are used as a source of alpha-radiation. It has been shown that activation processes predominate in all the three bioluminescent assay systems subjected to short-term exposure (20-55 h) and inhibition processes in the systems subjected to longer-term exposure to radiation. It has been found that these effects are caused by the radiation component of Am-241(3+) impact. The intensity of the Am-241(3+) effect on the bioluminescent assay systems has been shown to depend on the Am-241(3+) concentration, level of organization and integrity of the bioluminescent assay system. The bioluminescent assay systems in vivo have been found to be highly sensitive to Am-241(3+) (up to 10(-17) M).

Держатели документа:
SB RAS, Inst Biophys, Krasnoyarsk 660036, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Rozhko, T.V.; Kudryasheva, N.S.; Kuznetsov, A.M.; Vydryakova, G.A.; Bondareva, L.G.; Bolsunovsky, A.Y.

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11.

Long-term experiments on man's stay in biological life-support system / I. I. Gitelson [et al.] // Advances in Space Research. - 1989. - Vol. 9, Is. 8. - P65-71 . - ISSN 0273-1177
Кл.слова (ненормированные):
Agricultural Products -- Ecosystems -- Food Products -- Personnel - Health -- Space Flight - Manned Flight -- BIOS-3 Complex -- Phytotrons -- Spacecraft -- water -- air conditioning -- article -- biomass -- crop -- evaluation -- growth, development and aging -- human -- instrumentation -- light -- metabolism -- methodology -- microclimate -- photosynthesis -- plant -- waste management -- wheat -- Air Conditioning -- Biomass -- Crops, Agricultural -- Ecological Systems, Closed -- Evaluation Studies -- Humans -- Life Support Systems -- Light -- Photosynthesis -- Plants -- Triticum -- Waste Management -- Water
Аннотация: We describe the experimental system having maximal possible closure of material recycling in an ecosystem, including people and plants, which was carried out in a hermetically sealed experimental complex "BIOS-3", 315 m2 in volume. The system included 2 experimentators and 3 phytotrons with plants (total sowing area of 63 m2). Plants were grown with round-the-clock lamp irradiation with 130 Wm-2 PAR intensity. The plants production was food for people. Water exchange of ecosystem, as well as gas exchange, was fully closed excluding liquids and gas samples taken for chemical analysis outside the system. The total closure of material turnover constituted 91%. Health state of the crew was estimated before, during and after the experiment. A 5-months period did not affect their health. The experiments carried out prove that the closed ecosystem of "man-plants" is a prototype of a life-support system for long-term space expeditions. В© 1989.

Scopus
Держатели документа:
Institute of Biophysics, U.S.S.R. Academy of Sciences, Siberian Branch, Krasnoyarsk, 600366, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, I.I.; Terskov, I.A.; Kovrov, B.G.; Lisovskii, G.M.; Okladnikov, Yu.N.; Sid'ko, F.Ya.; Trubachev, I.N.; Shilenko, M.P.; Alekseev, S.S.; Pan'kova, I.M.; Tirranen, L.S.

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12.

Preparation and X-ray crystallographic analysis of recombinant obelin crystals diffracting to beyond 1.1 angstrom [Text] / E. S. Vysotski [et al.] // Acta Crystallogr. Sect. D-Biol. Crystallogr. - 2001. - Vol. 57. - P1919-1921, DOI 10.1107/S0907444901016523. - Cited References: 16 . - ISSN 0907-4449

РУБ Biochemical Research Methods + Biochemistry & Molecular Biology + Biophysics + Crystallography
Рубрики:
PHOTOPROTEIN AEQUORIN
   LONGISSIMA
   EVOLUTION
   CDNA
Аннотация: Crystals of recombinant obelin, the Ca2+-regulated photoprotein from the marine hydroid Obelia longissima, have been grown from a solution containing PEG 8000 and potassium phosphate. Hexamine-cobalt trichloride was used as an additive to increase the chance of crystallization. The crystals grow in a light yellow cubic form (0.5 x 0.5 x 0.45 mm) which diffracts to beyond 1.1 Angstrom resolution. The crystals belong to the space group C2, with unit-cell parameters a=83.43, b=54.92, c=52.99 Angstrom, beta = 112.00 degrees. The asymmetric unit contains one molecule. Crystals exposed to calcium ion before and after X-ray irradiation emit light, confirming that the crystals consist of an active photoprotein.

Держатели документа:
Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA
Russian Acad Sci, Siberian Branch, Inst Biophys, Photobiol Lab, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Vysotski, E.S.; Liu, Z.J.; Rose, J...; Wang, R.C.; Lee, J...

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13.

Comparison of chronic low-dose effects of alpha- and beta-emitting radionuclides on marine bacteria / M. A. Selivanova [et al.] // Cent. Eur. J. Biol. - 2014. - Vol. 9, Is. 10. - P951-959, DOI 10.2478/s11535-014-0331-0 . - ISSN 1644-3632
Кл.слова (ненормированные):
Am-241 -- Hormesis -- Luminous bacteria -- Peroxides -- Radiotoxicity -- Tritium
Аннотация: Effects of Americium-241 (241Am), alpha-emitting radionuclide of high specific radioactivity, and tritium (3H), beta-emitting radionuclide, on luminous bacteria Photobacterium phosphoreum were compared. Bioluminescence intensity served as a marker of bacterial physiological activity. Three successive stages in the bioluminescence response to 241Am and 3H were found under conditions of lowdose irradiation: (1) absence of effects, (2) activation, and (3) inhibition. They were interpreted in terms of bacterial response to stressfactor as stress recognition, adaptive response/syndrome, and suppression of physiological function (i.e. radiation toxicity). Times of bioluminescence activation (TBA) and inhibition (TBI) were suggested as parameters to characterize hormesis and toxic stages in a course of chronic low-dose irradiation of the microorganisms. Values of TBA and TBI of 241Am were shorter than those of 3H, revealing higher impact of alpha-irradiation (as compared to beta-irradiation) under comparable radiation doses. Increases of peroxide concentration and NADH oxidation rates in 241Am aquatic solutions were demonstrated; these were not found in tritiated water. The results reveal a biological role of reactive oxygen species generated in water solutions as secondary products of the radioactive decay. The study provides a scientific basis for elaboration of bioluminescence-based assay to monitor radiotoxicity of alpha- and beta-emitting radionuclides in aquatic solutions. © 2014 Versita Warsaw and Springer-Verlag Wien.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, 660036 Krasnoyarsk, Russian Federation
Siberian Federal University, 660041 Krasnoyarsk, Russian Federation
Siberian State Technological University, Lesosibirsk, Krasnoyarsk region, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Selivanova, M.A.; Rozhko, T.V.; Devyatlovskaya, A.N.; Kudryasheva, N.S.

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14.

STUDIES IN THE CO2 GAS-EXCHANGE OF WHEAT CENOSIS UNDER A CHANGE IN THE IRRADIATION LEVEL [Текст] / A. I. CHUCHALIN, F. Y. SIDKO, V. I. POLONSKY // FIZIOLOGIYA I BIOKHIMIYA KULTURNYKH RASTENII. - 1981. - Vol. 13, Is. 3. - P. 239-243. - Cited References: 12 . - ISSN 0256-1425

РУБ Plant Sciences + Horticulture

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
CHUCHALIN, A.I.; SIDKO, F.Y.; POLONSKY, V.I.

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15.

Fluorescence induced by light of 380-540 nm in leaves of cucumber depening on the vegetation time and irradiation regime [Текст] / V. V. Zavoruev, E. N. Zavorueva, A. V. Shelegov // Biofizika. - 2000. - Vol. 45, Is. 4. - P. 704-711. - Cited References: 25 . - ISSN 0006-3029

РУБ Biophysics
Рубрики:
CHLOROPHYLL
   INDUCTION
   RATIO
   INCREASE
Кл.слова (ненормированные):
slow fluorescence induction -- cucumber leaves -- vegetation time spectral conditions
Аннотация: The slow fluorescence induction produced iii cucumber leaves by light in the range of wavelengths 380-540 nm and intensity of 180 W/m(2) was studied. The ratio of fluorescence maxima in the red region (F(734)/F(682)) in young and mature leaves was approximately 2. It is assumed that this value depends on an increase in the contribution of the long-wavelength fluorescence due to the spillover effect. In plants grown under natural conditions, the paramster F(734)/F(682) correlated with the concentrations of chlorophyll and carotenoids. In plants grown hi the light of red and blue regions no such correlation was observed and the F(734)/F(682) remained unchanged. It is concluded that the F(534)/F(682) is affected by the intensity and spectral composition of exciting light used during the growing.

WOS
Держатели документа:
Russian Acad Sci, Inst Computat Modeling, Siberian Div, Krasnoyarsk 660036, Russia
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zavoruev, V.V.; Zavorueva, E.N.; Shelegov, A.V.

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16.

Stimulation of luminescence of mycelium of luminous fungus Neonothopanus nambi by ionizing radiation [Text] / T. V. Kobzeva [et al.] // Luminescence. - 2014. - Vol. 29, Is. 7. - P703-710, DOI 10.1002/bio.2656. - Cited References: 29. - The work was supported by the Program of Siberian Branch of Russian Academy of Sciences (project no. 71), Council for Grants of the President of the Russian Federation for Support of Leading Scientific Schools (project no. NSh 2272.2012.3), the Russian Foundation for Basic Research (project no. 12-03-33082), and the Program of Government of Russian Federation "On the Efforts for Attracting Leading Researchers to Educational Institutions of Russia" (grant no. 11.G34.31.0058). . - ISSN 1522-7235. - ISSN 1522-7243

РУБ Biochemistry & Molecular Biology
Рубрики:
BIOLUMINESCENCE
   COMPONENTS
   MECHANISMS
   SYSTEM
Кл.слова (ненормированные):
Higher luminous fungi -- Neonothopanus nambi -- ionizing irradiation -- reactive oxygen species -- lipid peroxidation
Аннотация: The luminescent system of higher luminous fungi is not fully understood and the enzyme/substrate pair of the light emission reaction has not been isolated. It was suggested that luminescence of fungi involves oxidase-type enzymes, and reactive oxygen species are important for fungal light production. Generation of reactive oxygen species can be stimulated by ionizing irradiation, which has not been studied for luminous fungi. We report the effect of X-irradiation on the luminescence of fungus Neonothopanus nambi. Experiments were performed withmyceliumon a home-built setup based on an X-ray tube and monochromator/photomultiplier tube. Application of X-rays does not change the emission spectrum, but after approximately 20 min of continuous irradiation, light production from unsupported mycelium starts growing and increases up to approximately five times. After peaking, its level decreases irrespective of the presence of X-irradiation. After staying at a certain level, light production collapses to zero, which is not related to the drying of the mycelium or thermal impact of radiation. The observed shape of kinetics is characteristic of a multistage and/or chain reaction. The time profile of light production must reflect the current levels of radicals present in the system and/or the activity of enzyme complexes involved in light production. Copyright (C) 2014 John Wiley & Sons, Ltd.

WOS
Держатели документа:
[Kobzeva, Tatiana V.
Melnikov, Anatoly R.
Karogodina, Tatiana Y.
Zikirin, Samat B.
Stass, Dmitri V.
Molin, Yuri N.] Inst Chem Kinet & Combust SB RAS, Novosibirsk 630090, Russia
[Melnikov, Anatoly R.
Zikirin, Samat B.
Stass, Dmitri V.] Novosibirsk State Univ, Novosibirsk 630090, Russia
[Rodicheva, Emma K.
Medvedeva, Svetlana E.
Puzyr, Alexey P.
Bondar, Vladimir S.
Gitelson, Joseph I.] Inst Biophys SB RAS, Krasnoyarsk 660036, Russia
[Rodicheva, Emma K.
Medvedeva, Svetlana E.
Puzyr, Alexey P.
Burov, Andrey A.
Bondar, Vladimir S.
Gitelson, Joseph I.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
[Burov, Andrey A.] Special Design Technol Bur Nauka SB RAS, Krasnoyarsk 660049, Russia
ИБФ СО РАН
СКТБ : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kobzeva, T.V.; Melnikov, A.R.; Karogodina, T.Y.; Zikirin, S.B.; Stass, D.V.; Molin, Y.N.; Rodicheva, E.K.; Medvedeva, S.E.; Puzyr, A.P.; Burov, A.A.; Bondar, V.S.; Gitelson, J.I.; Program of Siberian Branch of Russian Academy of Sciences [71]; Council for Grants of the President of the Russian Federation for Support of Leading Scientific Schools [NSh 2272.2012.3]; Russian Foundation for Basic Research [12-03-33082]; Program of Government of Russian Federation "On the Efforts for Attracting Leading Researchers to Educational Institutions of Russia" [11.G34.31.0058]

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17.

The levels of organization of the photosynthetic apparatus and the control of production processes in phytocenoses under artificial-light culture [Text] / A. A. Tikhomirov, G. M. Lisovskii // Russ. J. Plant Physiol. - 2001. - Vol. 48, Is. 3. - P. 395-399, DOI 10.1023/A:1016682904411. - Cited References: 21 . - ISSN 1021-4437

РУБ Plant Sciences

Кл.слова (ненормированные):
photosynthetic apparatus -- phytocenosis -- productivity -- artificial-light culture
Аннотация: The processes limiting the production in higher plant phytocenoses under an artificial-light culture are analyzed in relation to the multilevel organization of the photosynthetic apparatus (PA). The authors consider the feasibility of overcoming these limitations by optimizing the physical parameters of irradiation (the structure of the light spectrum, the rate, and the ratio of radiation fluxes in photosynthetically active radiation (PAR) and infrared (IR) regions) at the molecular, leaf, plant, and cenotic levels of PA organization. To illustrate this approach, the authors used a complex experiment in an artificial ecosystem to evaluate the efficiency of the light control of production processes in multispecies phytocenoses by alleviating or removing the factors that limit plant production at the various levels of PA organization. An artificial-light culture is seen as an instrument for solving several problems of theoretical and applied plant physiology and related disciplines in the future.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Lisovskii, G.M.

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18.

Light intensity and production parameters of phytocenoses cultivated on soil-like substrate under controled environment conditions [Text] / A. A. Tikhomirov [et al.] ; ed. M Nelson [et al.] // SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P. 1775-1780, DOI 10.1016/S0273-1177(03)00120-0. - Cited References: 11 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Ecology + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences
Рубрики:
SYSTEM
Аннотация: To increase the degree of closure of biological life support systems of a new generation, we used vermicomposting to involve inedible phytomass in the intra-system mass exchange. The resulting product was a soil-like substrate, which was quite suitable for growing plants (Manukovsky et al. 1996, 1997). However, the soil-like substrate can be regarded as a candidate for inclusion in a system only after a comprehensive examination of its physical, chemical, and other characteristics. An important criterion is the ability of the soil-like substrate to supply the necessary mineral elements to the photosynthesizing component under the chosen cultivation conditions. Thus, the purpose of this work was to study the feasibility of enhancing the production activity of wheat and radish crops by varying the intensity of photosynthetically active radiation, without decreasing the harvest index. The increase of light intensity from 920 to 1150 mumol(.)m(-2.)s(-1) decreased the intensity of apparent photosynthesis of the wheat crops and slightly increased the apparent photosynthesis of the radish crops The maximum total and grain productivity (kg/m(2)) of the wheat crops was attained at the irradiance of 920 mumol(.)m(-2.)s(-1). Light intensity of 1150 mumol(.)m(2.)s(-1) decreased the productivity of wheat plants and had no significant effect on the productivity of the radish crops (kg/m(2)) as compared to 920 mumol(.)m(-2.)s(-2). The qualitative and quantitative composition of microflora of the watering solution and substrate was determined by the condition of plants, developmental phase and light intensity. By the end of wheat growth under 1150 mumol(.)m(-2.)s(-1) the numbers of bacteria of the coliform family and phytopathogenic bacteria in the watering solution and substrate were an order of magnitude larger than under other illumination conditions. The obtained data suggest that the cultivation of plants in a life support system on soil-like substrate from composts has a number of advantages over the cultivation on neutral substrates, which require continual replenishment of the plant nutrient solution from the system's store to complement the macro- and micro- elements. Yet, a number of problems arise, including those related to the controlling of the production activity of the plants by the intensity of photosynthetically active radiation. It is essential to understand why the intensity of production processes is limited at higher irradiation levels and to overcome the factors responsible for this, so that the soil-like substrate could have an even better chance in the competition for the best plant cultivation technology to be used in biological life support systems. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
RAS SB, Inst Biophys, Krasnoyarsk 660036, Russia
Tomsk VV Kuibyshev State Univ, Tomsk 634050, Russia
Univ Clermont Ferrand, LGCB, F-63174 Aubiere, France
European Space Agcy, European Space Technol Ctr, NL-2200 AG Noordwijk, Netherlands
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Ushakova, S.A.; Gribovskaya, I.A.; Tirranen, L.S.; Manukovsky, N.S.; Zolotukhin, I.G.; Karnachuk, R.A.; Gros, J.B.; Lasseur, C...; Nelson, M \ed.\; Pechurkin, NS \ed.\; Dempster, WF \ed.\; Somova, LA \ed.\; Somo, , LA \ed.\

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19.

A comparative investigation of biodegradable polyhydroxyalkanoate films as matrices for in vitro cell cultures [Text] / E. I. Shishatskaya, T. G. Volova // J. Mater. Sci.-Mater. Med. - 2004. - Vol. 15, Is. 8. - P. 915-923, DOI 10.1023/B:JMSM.0000036280.98763.c1. - Cited References: 34 . - ISSN 0957-4530

РУБ Engineering, Biomedical + Materials Science, Biomaterials
Рубрики:
DEGRADATION
   POLY(3-HYDROXYBUTYRATE)
   POLYESTERS
   POLYMERS
Аннотация: The paper describes the production and investigation of flexible films made of high-purity polyhydroxyalkanoates (PHAs) - polyhydroxybutyrate [poly-(3HB)] and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate [poly(3Hl3-co-3HV)], containing 4-30 mol % hydroxyvalerate. Poly(3HB-co-3HV) films have a more porous structure than poly-(3HB) films, which are more compact, but their surface properties, such as wettability and surface and interface energies, are the same. Sterilisation of the PHA films by conventional methods (heat treatment and gamma-irradiation) did not impair their strength. Cells cultured on PHA films exhibited high levels of cell adhesion. Cell morphology, protein synthesis and DNA synthesis were estimated by extent of H-3-thymidine incorporation into the animal cell cultures of various origins (fibroblasts, endothelium cells, and isolated hepatocytes) in direct contact with PHAs. The investigation showed that this material can be used to make matrices for in vitro proliferous cells. The investigated properties of poly-(3HB) and poly(3HB-co-3HV) films proved to be fundamentally similar. (C) 2004 Kluwer Academic Publishers.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 60036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.

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20.

Effect of low-dose ionizing radiation on luminous marine bacteria: radiation hormesis and toxicity [Text] / N. S. Kudryasheva, T. V. Rozhko // J. Environ. Radioact. - 2015. - Vol. 142. - P68-77, DOI 10.1016/j.jenvrad.2015.01.012. - Cited References:131. - This work was supported by the Russian Foundation for Basic Research, Grant No.13-04-01305a, the Program "Molecular and Cellular Biology" of the Russian Academy of Sciences, project VI 57.1.1. The part of the work (review of effects of americium-241) was supported by the Russian Science Foundation, Grant No. 14-14-00076. . - ISSN 0265-931X. - ISSN 1879-1700

РУБ Environmental Sciences
Рубрики:
RECOMBINANT LUMINESCENT MICROORGANISMS
PHOTOBACTERIUM-LEIOGNATHI
Кл.слова (ненормированные):
Marine bacteria -- Low-dose effects -- Radiation hormesis -- Radiotoxicity -- Reactive oxygen species
Аннотация: The paper summarizes studies of effects of alpha- and beta-emitting radionuclides (americium-241, uranium-235+238, and tritium) on marine microorganisms under conditions of chronic low-dose irradiation in aqueous media. Luminous marine bacteria were chosen as an example of these microorganisms; bioluminescent intensity was used as a tested physiological parameter. Non-linear dose-effect dependence was demonstrated. Three successive stages in the bioluminescent response to americium-241 and tritium were found: 1 - absence of effects (stress recognition), 2 - activation (adaptive response), and 3 - inhibition (suppression of physiological function, i.e. radiation toxicity). The effects were attributed to radiation hormesis phenomenon. Biological role of reactive oxygen species, secondary products of the radioactive decay, is discussed. The study suggests an approach to evaluation of non-toxic and toxic stages under conditions of chronic radioactive exposure. (C) 2015 Elsevier Ltd. All rights reserved.

WOS
Держатели документа:
Inst Biophys SB RAS, Krasnoyarsk 660036, Russia
Siberian Fed Univ, Krasnoyarsk 660041, Russia
Krasnoyarsk State Med Acad, Krasnoyarsk 660022, Russia

Доп.точки доступа:
Kudryasheva, N. S.; Rozhko, T. V.; Russian Foundation for Basic Research [13-04-01305a]; Program "Molecular and Cellular Biology" of the Russian Academy of Sciences [VI 57.1.1]; Russian Science Foundation [14-14-00076]

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