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Salinity modulates thermotolerance, energy metabolism and stress response in amphipods Gammarus lacustris / K. P. Vereshchagina [et al.] // PeerJ. - 2016. - Vol. 2016, Is. 11, DOI 10.7717/peerj.2657 . - ISSN 2167-8359
Кл.слова (ненормированные):
Adaptation -- Amphipoda -- Gammarus lacustris -- Salinity -- Thermal tolerance
Аннотация: Temperature and salinity are important abiotic factors for aquatic invertebrates. We investigated the influence of different salinity regimes on thermotolerance, energy metabolism and cellular stress defense mechanisms in amphipods Gammarus lacustris Sars from two populations. We exposed amphipods to different thermal scenarios and determined their survival as well as activity of major antioxidant enzymes (peroxidase, catalase, glutathione S-transferase) and parameters of energy metabolism (content of glucose, glycogen, ATP, ADP, AMP and lactate). Amphipods from a freshwater population were more sensitive to the thermal challenge, showing higher mortality during acute and gradual temperature change compared to their counterparts from a saline lake. A more thermotolerant population from a saline lake had high activity of antioxidant enzymes. The energy limitations of the freshwater population (indicated by low baseline glucose levels, downward shift of the critical temperature of aerobic metabolism and inability to maintain steady-state ATP levels during warming) was ob- served, possibly reflecting a trade-off between the energy demands for osmoregulation under the hypo-osmotic condition of a freshwater environment and protection against temperature stress. © 2016 Vereshchagina et al.

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Держатели документа:
Institute of Biology, Irkutsk State University, Irkutsk, Russian Federation
Baikal Research Centre, Irkutsk, Russian Federation
Institute of Biophysics SB RAS, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute for Biological Sciences, University of Rostock, Rostock, Germany

Доп.точки доступа:
Vereshchagina, K. P.; Lubyaga, Y. A.; Shatilina, Z.; Bedulina, D.; Gurkov, A.; Axenov-Gribanov, D. V.; Baduev, B.; Kondrateva, E. S.; Gubanov, M.; Zadereev, E.; Sokolova, I.; Timofeyev, M.

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Effect of viscosity on efficiency of enzyme catalysis of bacterial luciferase coupled with lactate dehydrogenase and NAD(P)H:FMN-Oxidoreductase / O. S. Sutormin [et al.] // Mol. Cat. - 2018. - Vol. 458. - P60-66, DOI 10.1016/j.mcat.2018.08.012 . - ISSN 2468-8231
Кл.слова (ненормированные):
Bioluminescence -- Coupling of enzymes -- In vivo simulated media -- Metabolic chain -- Protein stability
Аннотация: One of the current trends of the modern biology figures out cellular enzyme behaviour. Numerous researches look more closely at the chemical composition of creating in vivo simulated media conditions. The aim of this work was to find out a thermodynamic cooperativity of enzymes in a triple-enzyme chain (lactate dehydrogenase + NAD(P)H: FMN-oxidoreductase + bacterial luciferase) under in vivo simulated condition. The thermodynamic cooperativity effects were found out based on the influence of the viscogens (glycerol and sucrose) on the thermal stability of the triple-enzyme system. The results showed that the viscogens do not lead to an increase in the thermal stability of the triple-enzyme system. In addition, organic solvents (sucrose and glycerol) added as viscous agents to the reaction medium altered the kinetics of this triple-enzyme chain, including changing the light emission decay constant (kdec) and quantum yield of luminescence (Q). Plus, sucrose was found to be more efficient in limiting the flexibility of enzymes than glycerol. The high sensitivity of the triple-enzyme system to the viscogens may be connected with a fact that lactate dehydrogenase does not bound with couple enzyme system NAD(P)H: FMN-oxidoreductase + bacterial luciferase inside the real cell. Since this approach may be used as a method to understand the real connection between enzymes in cellular multi-enzyme metabolic chains inside the luminous bacteria cell. © 2018 Elsevier B.V.

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Держатели документа:
Department of Biophysics, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Sutormin, O. S.; Sukovataya, I. E.; Pande, S.; Kratasyuk, V. A.

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Nonspecific stress response to temperature increase in Gammarus lacustris Sars with respect to oxygen-limited thermal tolerance concept / K. Vereshchagina [et al.] // PeerJ. - 2018. - Vol. 6. - Ст. e5571, DOI 10.7717/peerj.5571. - Cited References:49. - The study was carried out with the main financial support of Russian Science Foundation grant 17-14-01063, with the partial financial support of Russian Foundation for Basic Research grants 16-34-00687, 16-34-60060, 17-34-50012, the base part of Goszadanie project 6.9654.2017/8.9, joint program of DAAD and Ministry of education and Science M. Lomonosov (6.12735.2018/12.2) and Lake Baikal Foundation (FOB_02-3/05). There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. . - ISSN 2167-8359

РУБ Multidisciplinary Sciences
Рубрики:
COPEPOD TIGRIOPUS-JAPONICUS
SHUNET SOUTH SIBERIA
HEAT-SHOCK PROTEINS
Кл.слова (ненормированные):
Gammarus lacustris -- Heat shock proteins 70 (HSP70) -- Nonspecific cellular -- stress-response (NCSR) -- Lactate dehydrogenase -- Diene conjugates -- Schiff -- bases -- Triene conjugates
Аннотация: The previously undescribed dynamics of the heat shock protein HSP70 and subsequent lipid peroxidation products have been assessed alongside lactate dehydrogenase activity for Gammarus lacustris Sars, an amphipod species from the saltwater Lake Shira (Republic of Khakassia). Individuals were exposed to a gradual temperature increase of 1 degrees C/hour (total exposure duration of 26 hours) starting from the mean annual temperature of their habitat (7 degrees C) up to 33 degrees C. A complex of biochemical reactions occurred when saltwater G. lactustris was exposed to the gradual changes in temperature. This was characterized by a decrease in lactate dehydrogenase activity and the launching of lipid peroxidation. The HSP70 level did not change significantly during the entire experiment. In agreement with the concept of oxygen-limited thermal tolerance, an accumulation of the most toxic lipid peroxides (triene conjugates and Schiff bases) in phospholipids occurred at the same time and temperature as the accumulation of lactate. The main criterion overriding the temperature threshold was, therefore, the transition to anaerobiosis, confirmed by the elevated lactate levels as observed in our previous associated study, and by the development of cellular stress, which was expressed by an accumulation of lipid peroxidation products. An earlier hypothesis, based on freshwater individuals of the same species, has been confirmed whereby the increased thermotolerance of G. lacustris from the saltwater lake was caused by differences in energy metabolism and energy supply of nonspecific cellular stress-response mechanisms. With the development of global climate change, these reactions could be advantageous for saltwater G. lacustris. The studied biochemical reactions can be used as biomarkers for the stress status of aquatic organisms when their habitat temperature changes.

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Держатели документа:
Irkutsk State Univ, Inst Biol, Irkutsk, Russia.
Baikal Res Ctr, Irkutsk, Russia.
Belarusian State Univ, Int Sakharov Environm Inst, Minsk, BELARUS.
SB RAS, Inst Biophys, Krasnoyarsk Res Ctr, Krasnoyarsk, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.

Доп.точки доступа:
Vereshchagina, Kseniya; Kondrateva, Elizaveta; Axenov-Gribanov, Denis; Shatilina, Zhanna; Khomich, Andrey; Bedulina, Daria; Zadereev, Egor; Timofeyev, Maxim; Russian Science Foundation [17-14-01063]; Russian Foundation for Basic Research [16-34-00687, 16-34-60060, 17-34-50012]; Goszadanie project joint program of DAAD [6.9654.2017/8.9]; Ministry of education and Science M. Lomonosov [6.12735.2018/12.2]; Lake Baikal Foundation [FOB_02-3/05]

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Enzymatic bioassay of soil: Sensitivity comparison of mono-, double- And triple-enzyme systems to soil toxicants / O. S. Sutormin [и др.] // Tsitologiya. - 2018. - Vol. 60, Is. 10. - С. 826-829, DOI 10.7868/S0041377118100132 . - ISSN 0041-3771
Кл.слова (ненормированные):
Bacterial luciferase -- Bioluminescent analysis -- Coupled enzyme systems -- Ecological monitoring -- Enzymatic toxicity bioassays -- Lactate dehydrogenase -- NADH:FMN-oxidoreductase -- Soil
Аннотация: In this paper, we have investigated the possibilities of application of enzymatic systems with increasing chain length as a bioassay to evaluate the soil contamination status. The sensitivity of monoenzyme reaction as well as double- and triple-enzyme chains based on NAD(P)H:FMN-oxidoreductase and luciferase of luminous bacteria and lactate dehydrogenase to pesticides and copper ions in water and water extracts from soils were estimated. For this, the toxicological parameter IC 20 reflecting the sensitivity limit of the enzyme system to the to-xicant was used. It was revealed that elongation of the coupled enzyme chain (from mono- to triple-enzyme) increases the sensitivity of the bioassay, in some cases by several orders of magnitude. This pattern can be used as a tool to improve the properties of enzymic bioassays. The effect of extracts from uncontaminated soils of various types on enzymatic systems also differs, which makes possible to design the specialized enzymatic bioassays as well. © 2018 Sankt Peterburg.All rights reserved.

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Держатели документа:
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics Siberian Branch of RAS, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Sutormin, O. S.; Kolosova, E. M.; Nemtseva, A. V.; Iskorneva, I. V.; Lisitsa, A. A.; Matvienko, V. S.; Esimbekova, A. N.; Kratasyuk, V. A.

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Set of Enzymatic Bioassays for Assessment of Soil Contamination / E. M. Kolosova, O. S. Sutormin, E. N. Esimbekova [et al.] // Dokl. Biol. Sci. - 2019. - Vol. 489, Is. 1. - P165-168, DOI 10.1134/S0012496619060024 . - ISSN 1608-3105
Аннотация: A concept of the comprehensive assessment of soil contamination is proposed. According to it, the conclusion regarding the presence of toxic substances in the analyzed sample is based on the inhibition of enzymatic reactions responsible for various functions of a living organism, such as luminescence, respiration, etc. These functions are taken as test functions in classical bioassays with the use of living objects (luminous bacteria, daphnia, algae, and others). The regularities of the impact of different classes of toxicants on the activity of particular enzymes or coupled oligo-enzyme chains have been established. These enzyme reactions are selected as potential test objects: markers of contamination. Three enzyme systems with the maximal sensitivity to different classes of toxicants have been chosen for the set of enzymatic bioassays: butyrylcholinesterase, NAD(P)H:FMN-oxidoreductase + luciferase, and lactate dehydrogenase + NAD(P)H:FMN-oxidoreductase + luciferase. The possibility to use enzymes instead of living organisms in the bioassay of natural complex systems has been shown.

Scopus
Держатели документа:
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Kolosova, E. M.; Sutormin, O. S.; Esimbekova, E. N.; Lonshakova-Mukina, V. I.; Kratasyuk, V. A.

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The role of acidosis in the pathogenesis of severe forms of COVID-19 / Y. D. Nechipurenko, D. A. Semyonov, I. A. Lavrinenko [et al.] // Biology. - 2021. - Vol. 10, Is. 9. - Ст. 852, DOI 10.3390/biology10090852 . - ISSN 2079-7737
Кл.слова (ненормированные):
Acidosis -- Bohr effect -- COVID-19 -- Hypoxia -- Lactate -- PH -- SARS-CoV-2 -- Saturation
Аннотация: COVID-19 has specific characteristics that distinguish this disease from many other infec-tions. We suggest that the pathogenesis of severe forms of COVID-19 can be associated with acidosis. This review article discusses several mechanisms potentially linking the damaging effects of COVID-19 with acidosis and shows the existence of a vicious cycle between the development of hypoxia and acidosis in COVID-19 patients. At the early stages of the disease, inflammation, difficulty in gas exchange in the lungs and thrombosis collectively contribute to the onset of acidosis. In accordance with the Verigo-Bohr effect, a decrease in blood pH leads to a decrease in oxygen saturation, which contributes to the exacerbation of acidosis and results in a deterioration of the patient’s condition. A decrease in pH can also cause conformational changes in the S-protein of the virus and thus lead to a decrease in the affinity and avidity of protective antibodies. Hypoxia and acidosis lead to dysregu-lation of the immune system and multidirectional pro-and anti-inflammatory reactions, resulting in the development of a “cytokine storm”. In this review, we highlight the potential importance of supporting normal blood pH as an approach to COVID-19 therapy. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

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Держатели документа:
Laboratory DNA-Protein Recognition, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russian Federation
Institute of Molecular Medicine and Pathobiochemistry, Voyno-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, 660022, Russian Federation
Institute of Biophysics Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Department of Human and Animal Physiology, Faculty of Medicine and Biology, Voronezh State University, Voronezh, 394018, Russian Federation
Department of Biological and Medical Physics, Moscow Institute of Physics and Technology, Moscow Region, Dolgoprudny, 141701, Russian Federation
Department of Biophysics, Faculty of Physics, Lomonosov Moscow State University, Moscow, 119991, Russian Federation
Laboratory of Medical Analytical Methods and Devices, Institute for Analytical Instrumentation of the Russian Academy of Sciences, St. Petersburg, 198095, Russian Federation
Sendai Viralytics LLC, Acton, MA 117261, United States
Laboratory of Cellular Bases for the Development of Malignant Diseases, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russian Federation

Доп.точки доступа:
Nechipurenko, Y. D.; Semyonov, D. A.; Lavrinenko, I. A.; Lagutkin, D. A.; Generalov, E. A.; Zaitceva, A. Y.; Matveeva, O. V.; Yegorov, Y. E.

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The Role of Acidosis in the Pathogenesis of Severe Forms of COVID-19 / Y. D. Nechipurenko, D. A. Semyonov, I. A. Lavrinenko [et al.] // Biology-Basel. - 2021. - Vol. 10, Is. 9. - Ст. 852, DOI 10.3390/biology10090852. - Cited References:86. - This research was funded by the Presidium of the Russian Academy of Sciences for Molecular and Cellular Biology and the Program of Fundamental Research for State Academies for years 2013-2020, project no. 01201363818. . - ISSN 2079-7737

РУБ Biology
Рубрики:
RESPIRATORY-ACIDOSIS
   LACTATE
   COAGULATION
   GLYCOLYSIS
   SECRETION
Кл.слова (ненормированные):
SARS-CoV-2 -- COVID-19 -- acidosis -- hypoxia -- saturation -- Bohr effect -- lactate -- pH
Аннотация: Simple Summary Recently, several studies have shown that acidosis, which is increased acidity in the blood and other body tissues, is often associated with severe COVID-19. In this article, we look at the mechanisms and consequences of acidosis that can lead to an exacerbation of COVID-19. We want to draw the attention of readers to the threshold values of such disease characteristics as hypoxia and acidosis, which are associated with a sharp deterioration in the patient's condition. Hypoxia and acidosis mutually reinforce each other according to the principle of a vicious cycle (that is, they are involved in a system of positive feedbacks). Elevated blood lactate (lactic acid) levels are associated with poor clinical outcomes in COVID patients. As a practical recommendation, we propose to pay more attention to the prevention of acidosis, including in the early stages of the disease, when the adjustment of homeostasis requires less effort and is less risky. COVID-19 has specific characteristics that distinguish this disease from many other infections. We suggest that the pathogenesis of severe forms of COVID-19 can be associated with acidosis. This review article discusses several mechanisms potentially linking the damaging effects of COVID-19 with acidosis and shows the existence of a vicious cycle between the development of hypoxia and acidosis in COVID-19 patients. At the early stages of the disease, inflammation, difficulty in gas exchange in the lungs and thrombosis collectively contribute to the onset of acidosis. In accordance with the Verigo-Bohr effect, a decrease in blood pH leads to a decrease in oxygen saturation, which contributes to the exacerbation of acidosis and results in a deterioration of the patient's condition. A decrease in pH can also cause conformational changes in the S-protein of the virus and thus lead to a decrease in the affinity and avidity of protective antibodies. Hypoxia and acidosis lead to dysregulation of the immune system and multidirectional pro- and anti-inflammatory reactions, resulting in the development of a "cytokine storm". In this review, we highlight the potential importance of supporting normal blood pH as an approach to COVID-19 therapy.

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Держатели документа:
Russian Acad Sci, Engelhardt Inst Mol Biol, Lab DNA Prot Recognit, Moscow 119991, Russia.
Voyno Yasenetsky Krasnoyarsk State Med Univ, Inst Mol Med & Pathobiochem, Krasnoyarsk 660022, Russia.
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.
Voronezh State Univ, Fac Med & Biol, Dept Human & Anim Physiol, Voronezh 394018, Russia.
Moscow Inst Phys & Technol, Dept Biol & Med Phys, Dolgoprudnyi 141701, Russia.
Lomonosov Moscow State Univ, Fac Phys, Dept Biophys, Moscow 119991, Russia.
Russian Acad Sci, Lab Med Analyt Methods & Devices, Inst Analyt Instrumentat, St Petersburg 198095, Russia.
Sendai Viralyt LLC, Acton, MA USA.
Russian Acad Sci, Engelhardt Inst Mol Biol, Lab Cellular Bases Dev Malignant Dis, Moscow 119991, Russia.

Доп.точки доступа:
Nechipurenko, Yury D.; Semyonov, Denis A.; Lavrinenko, Igor A.; Lagutkin, Denis A.; Generalov, Evgenii A.; Zaitceva, Anna Y.; Matveeva, Olga, V; Yegorov, Yegor E.; Lagutkin, Denis; Presidium of the Russian Academy of Sciences for Molecular and Cellular Biology; Program of Fundamental Research for State Academies for years 2013-2020 [01201363818]

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