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Model assessment of the migration capability of transuranium radionuclides (241Am and Pu isotopes) and 152Eu in the system bottom sediments-Yenisei river water by chemical fractionation technique / L. G. Bondareva [et al.] // Radiochemistry. - 2005. - Vol. 47, Is. 4. - P415-421, DOI 10.1007/s11137-005-0112-8 . - ISSN 1066-3622
Аннотация: Sorption of radiotracers 241Am and 242Pu and weighable amounts of uranium and stable Eu on bottom sediments in the simulated system bottom sediments-Yenisei river water was studied. At a contact time of 40 min these metals are completely sorbed on the bottom sediments, presumably via association with organomineral complexes and hydrated gels. Along with sorption, these metals can form soluble complexes and stable colloids (pseudocolloids). The distribution factors of 241Am and 242Pu, and stable Eu in the simulated system between bottom sediments and liquid phases were calculated from the results of sorption experiments. The introduced 241Am and 152Eu initially present in radioactively contaminated bottom sediments of the Yenisei river show similar pattern of distribution over differently mobile fractions. The distribution of weighable amounts of stable Eu significantly differs from that of initially present 152Eu and tracer 241Am due to decelerated sorption of stable Eu and its considerably higher concentration. A significant part of 242Pu, 241Am, and 152Eu is associated with mobile fractions of bottom sediments, which, under certain environmental conditions, can pass into river water as migrating species. В©2005 Pleiades Publishing, Inc.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Joint Institute of Geology, Geophysics, and Mineralogy, Siberian Division, Russian Academy of Sciences, Novosibirsk, Russian Federation
Institute of Chemistry and Chemical Technology, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bondareva, L.G.; Bolsunovskii, A.Ya.; Sukhorukov, F.V.; Kazbanov, V.I.; Makarova, I.V.; Legler, E.V.

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The effect of radionuclide and heavy metal contamination of the Yenisei River on cytogenetics of aquatic plant Elodea canadensis / A. Bolsunovsky [et al.] // Radioprotection. - 2009. - Vol. 44, Is. 5. - P83-88, DOI 10.1051/radiopro/20095021 . - ISSN 0033-8451
Аннотация: The study was done to determine concentrations of radionuclides and heavy metals and to evaluate the frequency of chromosomal aberrations in samples of Elodea canadensis, a submerged plant, collected in different parts of the Yenisei River. Samples were collected in the area subjected to radioactive impact of theMining-and-Chemical Combine (MCC) at Zheleznogorsk and in the control areas, upstream of the MCC. The investigations showed that Elodea biomass in the area affected by MCC operation contained a long inventory of artificial radionuclides typical of the MCC discharges. Upstream of the MCC, in the control sampling areas, the sediments and the Elodea biomass contained only one artificial radionuclide - 137Cs. Thus, the exposure doses to Elodea shoots and roots upstream of the MCC are small (not more than 8 ?Gy/d) and the main contribution to them is made by natural radionuclides. At the MCC discharge site (the village of Atamanovo) and downstream of it, the total dose rate increases almost an order of magnitude, reaching its maximal values - 72 ?Gy/d for Elodea shoots and 58 ? Gy/d for roots. Cytogenetic investigations of Elodea roots showed that at the MCC discharge site (the village of Atamanovo) and downstream of it the occurrence of chromosomal aberrations in ana-telophase and metaphase cells of Elodea was considerably higher than in the control area. It is highly probable that this simultaneous dramatic increase in the total exposure rate and the occurrence of chromosomal aberrations in Elodea is associated with the radiation factor. It is suggested that Elodea is affected not only by the radiation factor but also by the chemical factor - toxicity of heavy metals. В© 2009 EDP Sciences.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russian Federation
Siberian Federal University, 79 Svobodnyi Ave., 660041 Krasnoyarsk, Russian Federation
Institute of Forest, Siberian Branch, Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.; Muratova, E.; Sukovaty, A.; Kornilova, M.

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Influence of anthropogenic pollution on content of essential polyunsaturated fatty acids in links of food chain of river ecosystem / M. I. Gladyshev [et al.] // Contemporary Problems of Ecology. - 2012. - Vol. 5, Is. 4. - P376-385, DOI 10.1134/S1995425512040051 . - ISSN 1995-4255
Кл.слова (ненормированные):
gammarus -- grayling -- heavy metals -- polyunsaturated fatty acids -- amphipod -- anthropogenic effect -- anthropogenic source -- fatty acid -- food chain -- heavy metal -- littoral environment -- oil pollution -- phenol -- river system -- salmonid -- sampling -- Krasnoyarsk [Russian Federation] -- Russian Federation -- Yenisei River -- Gammarus
Аннотация: In the course of monthly sampling in 2008-2010, two regions of the littoral of the Yenisei river were compared. One of these regions (conventionally pure) was situated upstream of Krasnoyarsk, while the other (conventionally polluted) was downstream of Krasnoyarsk. The concentrations of heavy metals, oil products, phenols, biogenic elements and essential polyunsaturated fatty acids (PUFAs) in various components of the river ecosystem were determined. It was discovered that the anthropogenic pollution causes a decrease in the resources of essential PUFA in the biomass of the upper links of the food chain of the river ecosystem. В© 2012 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, Svobodnii prosp. 79, Krasnoyarsk 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Anishchenko, O.V.; Sushchnik, N.N.; Kalacheva, G.S.; Gribovskaya, I.V.; Ageev, A.V.

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Seasonal variations of metal concentrations in periphyton and taxonomic composition of the algal community at a Yenisei River littoral site / O. V. Anishchenko [et al.] // Central European Journal of Biology. - 2010. - Vol. 5, Is. 1. - P125-134, DOI 10.2478/s11535-009-0060-y . - ISSN 1895-104X
Кл.слова (ненормированные):
Heavy metals -- Periphyton -- Seasonal dynamics -- algae -- Chlorophyta -- Cyanobacteria -- Ulothrix zonata
Аннотация: The concentrations of metals K, Na, Ca, Mg, Fe, Mn, Zn, Cu, Ni, Pb, Co and Cr, in the water and periphyton (epilithic algal communities) were studied at a site in the middle stream of the Yenisei River (Siberia, Russia) during three years using monthly sampling frequencies. Despite considerable seasonal variations in aquatic concentrations of some metals, there was no correlation between metal contents in the water and in periphyton. Seasonal concentration variations of some metals in periphyton were related to the species (taxonomic) composition of periphytic microalgae and cyanobacteria. Enhanced levels of Ni and Co in periphyton in late autumn, winter, and early spring were likely caused by the predominance of cyanobacteria in the periphytic community, and annual maximum levels of K in periphyton in late spring and early summer were attributed to the domination of Chlorophyta, primarily Ulothrix zonata. В© Versita Warsaw and Springer-Verlag Berlin Heidelberg.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, 660036 Krasnoyarsk, Russian Federation
Siberian Federal University, 660041 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Anishchenko, O.V.; Gladyshev, M.I.; Kravchuk, E.S.; Ivanova, E.A.; Gribovskaya, I.V.; Sushchik, N.N.

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Distribution and migration of metals in trophic chains of the Yenisei ecosystem near Krasnoyarsk City / O. V. Anishchenko [et al.] // Water Resources. - 2009. - Vol. 36, Is. 5. - P594-603, DOI 10.1134/S0097807809050121 . - ISSN 0097-8078
Кл.слова (ненормированные):
Aquatic organisms -- Atomic absorption -- Bulk concentration -- Cd concentrations -- Cr concentration -- Ecosystem components -- Emission spectral analysis -- Flame photometry -- Fly larvae -- International standards -- Periphytons -- Primary producers -- Waterbodies -- Aquaculture -- Cadmium -- Chromium -- Ecology -- Photometry -- Spectroscopy -- Spectrum analysis -- Spectrum analyzers -- Water absorption -- Water analysis -- Concentration (process) -- aluminum -- aquatic ecosystem -- aquatic organism -- cadmium -- chromium -- concentration (composition) -- copper -- pollutant transport -- river pollution -- trophic environment -- Krasnoyarsk [Russian Federation] -- Russian Federation -- Yenisei River -- Bryophyta -- Decapoda (Crustacea) -- Thymallus arcticus
Аннотация: Methods of atomic absorption, flame photometry, and emission spectral analysis were used to study the concentrations of metals in water and major ecosystem components of the Yenisei River upstream of Krasnoyarsk City (conventionally background area). The mean bulk concentrations of Al and Cu in water exceeded the MAC for water bodies used for fishery. Cu concentration in freshwater shrimp was found to be reliably higher than that in the link of primary producers (periphyton), and Cd concentration in caddis fly larvae was found to exceed that in water moss. The maximal concentrations of metals among the examined aquatic organisms were recorded in periphyton. Cr concentration in the muscles of Arctic grayling was found to exceed some international standards. В© 2009 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Geophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, prosp. Svobodnyi 79, Krasnoyarsk 660046, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Anishchenko, O.V.; Gladyshev, M.I.; Kravchuk, E.S.; Sushchik, N.N.; Gribovskaya, I.V.

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Benefit-risk ratio of food fish intake as the source of essential fatty acids vs. heavy metals: A case study of Siberian grayling from the Yenisei River / M. I. Gladyshev [et al.] // Food Chemistry. - 2009. - Vol. 115, Is. 2. - P545-550, DOI 10.1016/j.foodchem.2008.12.062 . - ISSN 0308-8146
Кл.слова (ненормированные):
Benefit-risk ratio -- Essential fatty acids -- Grayling -- Hazard quotient -- Heavy metals
Аннотация: A formula was derived for quantification of benefit-risk ratio (hazard quotient) for the intake of a product containing essential polyunsaturated fatty acids vs. heavy metals. The quotient was used in a three year case study of the contents of essential fatty acids and heavy metals in Siberian grayling, the main food fish from the middle section of the Yenisei River. As found, in general the fish intake was potentially very beneficial for human health, except on a few occasions, when the risk overweighed the benefit because of high contents of chromium in the fish muscle tissue. The data demonstrated the necessity for regular monitoring of the hazard quotients for food fish in wild conditions, based at least on monthly sampling frequency. В© 2008 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, Svobodny av. 79, Krasnoyarsk, 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Sushchik, N.N.; Anishchenko, O.V.; Makhutova, O.N.; Kalachova, G.S.; Gribovskaya, I.V.

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Metal Concentrations in the Ecosystem and Around Recreational and Fish-Breeding Pond Bugach / M. I. Gladyshev [et al.] // Water Resources. - 2001. - Vol. 28, Is. 3. - P288-296, DOI 10.1023/A:1010400807660 . - ISSN 0097-8078
Аннотация: Data of two field studies were used to analyze the concentrations of Na, K, Ca, Mg, Fe, Mn, Zn, Cu, Al, Cr, Ni, Cd, and Pb in the water, bottom deposits, zoobenthos, fish, and macrophytes of the Pond Bugach and in the soils near the pond. It was established that the majority of metals in the soils and bottom deposits correlate and their concentrations are governed by the universal geochemical factors of the region. The heavy metals were recognized that originate from anthropogenic sources and their concentrations were found to exceed the maximum admissible values for different components of the ecosystem. Five types of heavy metals migration were recognized in the soil-bottom deposits-zoobenthos-fish chain and in macrophytes. Statistically significant difference was found to exist between the concentrations of some heavy metals in the muscles of fish species with different food types (crucian carp and perch) as well as between the correlations of metals. The recorded concentrations were compared with the concentrations of metals measured in the last decade in other limnetic ecosystems in Siberia, Europe, North America, and China.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Krasnoyarsk Agricultural University, pr. Mira 88, Krasnoyarsk, 660049, Russian Federation
Krasnoyarsk State University, Svobodnyi prosp. 79, Krasnoyarsk, 660042, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Gribovskaya, I.V.; Ivanova, E.A.; Moskvichova, A.V.; Muchkina, E.Ya.; Chuprov, S.M.

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Content of metals in compartments of ecosystem of a Siberian pond / M. I. Gladyshev [et al.] // Archives of Environmental Contamination and Toxicology. - 2001. - Vol. 41, Is. 2. - P157-162, DOI 10.1007/s002440010233 . - ISSN 0090-4341
Кл.слова (ненормированные):
aluminum -- cadmium -- calcium -- chromium -- copper -- heavy metal -- iron -- lead -- magnesium -- manganese -- nickel -- potassium -- sodium -- zinc -- aquatic ecosystem -- biological uptake -- heavy metal -- pond -- article -- bioaccumulation -- ecosystem -- fish -- nonhuman -- pond -- priority journal -- Russian Federation -- sediment -- soil pollution -- water contamination -- Animals -- Ecosystem -- Environmental Monitoring -- Fishes -- Geologic Sediments -- Invertebrates -- Metals, Heavy -- Plants -- Water Pollutants -- Russian Federation
Аннотация: During three field seasons (June-September) of 1997-99 contents of Na, K, Ca, Mg, Fe, Mn, Zn, Cu, Al, Cr, Ni, Cd, and Pb were determined in compartments of ecosystem (surrounding soils, bottom sediments, water, zoobenthos, macrophytes, and fish) of a fish and recreation pond situated at the edge of Krasnoyarsk City (Siberia, Russia). Contents of most parts of metals in soils, water, and macrophytes significantly correlated with each other. As concluded, their contents were determined by natural, general, geochemical peculiarities of the region. Heavy metals, contents of which were higher than federal upper limits of concentration, were revealed. In muscles of fish with different feeding spectra - crucian and perch - concentrations of some metals differed significantly; correlation graphs for metals also had different structures. Comparison of our data with those on diverse aquatic ecosystems of Siberia, Europe, North America, and China published in the last decade was carried out. It was concluded that a distribution of heavy metals in the compartments of an aquatic ecosystem presently have to be determined for each particular water body until general regularities are discovered.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Krasnoyarsk State Agricultural University, Mira av., 88, Krasnoyarsk, 660049, Russian Federation
Krasnoyarsk State University, Svobodny av., 79, Krasnoyarsk, 660042, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Gribovskaya, I.V.; Moskvicheva, A.V.; Muchkina, E.Y.; Chuprov, S.M.; Ivanova, E.A.

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The influence of microbial associations on germination of wheat seeds and growth of seedlings under impact of zinc salts / L. A. Somova, N. S. Pechurkin // Advances in Space Research. - 2009. - Vol. 43, Is. 8. - P1224-1228, DOI 10.1016/j.asr.2008.12.008 . - ISSN 0273-1177
Кл.слова (ненормированные):
Heavy metals -- Life support system -- Microbial associations -- Microorganisms -- Wheat seeds -- Concentration (process) -- Heavy metals -- Metals -- Microorganisms -- Recycling -- Salts -- Seed -- Zinc -- Adverse effects -- Biological recycling -- Growth and development -- Heavy metal salts -- Higher plants -- Investigate effects -- Life support system -- Long-term missions -- Maximum permissible concentrations -- Microbial associations -- Pathogenic organisms -- Plantlets -- Recycling systems -- Root systems -- Wheat seeds -- Zinc salts -- Grain (agricultural product)
Аннотация: The life support systems (LSS) for long-term missions are to use cycling-recycling systems, including biological recycling. Higher plants are the traditional regenerator of air and producer of food. They should be used in many successive generations of their reproduction in LSS. Studies of influence of microbial associations on germination of wheat seeds and on growth of seedlings under impact of heavy metals are necessary because of migration of heavy metals in LSS. Microbial associations are able to stimulate growth of plants, to protect them from pathogenic organisms and from toxicity of heavy metal salts. The goal of this work was to investigate effect of microbial associations on the germination of wheat seeds and on the growth of seedlings under impact of different concentrations of ZnSO4. The results of investigations showed that:(1)Zinc salt had an adverse effect on germination of wheat seeds, beginning with concentrations of 8 MPC (Maximum Permissible Concentration) and higher.(2)Microbial associations (concentrations -104 to 107 cells/ml) were able to decrease (partly or completely) the adverse effect of ZnSO4 on germination of wheat seeds.(3)Concentrations (104-107 cells/ml) of microbial associations were able to decrease partly the adverse effect of zinc salts (intervals: from 1 to 32 MPS{cyrillic}) on the growth and development of wheat plantlets during heterotrophic phase.(4)The root system of plants was more sensitive to the adverse effect of ZnSO4 than shoots of plants. В© 2008 COSPAR.

Scopus
Держатели документа:
Institute of Biophysics SB, Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Somova, L.A.; Pechurkin, N.S.

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10.

Transfer of xenobiotics through cell membranes of luminous bacteria / S. E. Medvedeva // Luminescence. - 1999. - Vol. 14, Is. 5. - P267-270 . - ISSN 1522-7235
Кл.слова (ненормированные):
Luminous bacteria -- Toxicant -- Ultrastructure -- bacterial DNA -- edetic acid -- toluene -- xenobiotic agent -- article -- cell membrane -- DNA damage -- drug effect -- luminescence -- metabolism -- Photobacterium -- sensitivity and specificity -- transport at the cellular level -- ultrastructure -- Vibrio -- Biological Transport -- Cell Membrane -- DNA Damage -- DNA, Bacterial -- Edetic Acid -- Luminescence -- Photobacterium -- Sensitivity and Specificity -- Toluene -- Vibrio -- Xenobiotics
Аннотация: The influence of some chemical substances on luminous bacteria was studied to elucidate the interrelation between the xenobiotics action on bacterial luminescence and cell ultrastructure. Such substances as quinones, phenols, chlorides of heavy metals (in concentrations of substances inhibiting luminescence by 50%) resulted in damaging effects upon bacteria: a lot of cells had damage of membranes due to changes in their permeability. It was found that the high concentration of EDTA and toluene decreased the luminescence and caused the condensation of DNA-fibrils and the cell damage after long-term and short-term action. The low concentration of EDTA and toluene did not decrease the bacterial luminescence; the noticeable damage of cell membranes did not take place during short-term treatment. However, the long action of these substances changed the membrane permeability resulting in increased sensitivity of bacterial luminescence to some toxic substances. Copyright В© 1999 John Wiley & Sons, Ltd.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Medvedeva, S.E.

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11.

Bioluminescent method in studying the complex effect of sewage components / D. I. Stom [et al.] // Archives of Environmental Contamination and Toxicology. - 1992. - Vol. 22, Is. 2. - P203-208 . - ISSN 0090-4341
Кл.слова (ненормированные):
heavy metal -- phenol derivative -- quinone derivative -- article -- bacterium -- bioluminescence -- cell membrane -- nonhuman -- priority journal -- sludge -- ultrastructure -- Benzoquinones -- Catechin -- Hydroquinones -- Luminescent Measurements -- Metals -- Phenol -- Phenols -- Photobacterium -- Sewage -- Vibrio -- Water Pollutants, Chemical -- Bacteria (microorganisms)
Аннотация: The inhibition of bacterial luminescence has been used in testing industrial enterprises sewage. The toxicity of the sewage is less than the total toxicity of separate components due to neutralization of quinone products of polyphenol oxidation in the reactions with the other phenol components of sewage. Toxicity increase is due to their influence on the cell membrane. Studies of cell ultrastructure confirm this fact. The studied mechanism of the complex effect allowed a more accurate forecast of the ecological situation during the discharge of phenol compounds and metals. It also showed the necessity of taking into account the complex effect of sewage components on contaminant discharge into water reservoirs.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Stom, D.I.; Geel, T.A.; Balayan, A.E.; Shachova, G.I.; Kuznetsov, A.M.; Medvedeva, S.E.

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12.

Effect of different salts and detergents on luciferin-luciferase luminescence of the enchytraeid Fridericia heliota / N. S. Rodionova, V. N. Petushkov // Journal of Photochemistry and Photobiology B: Biology. - 2006. - Vol. 83, Is. 2. - P123-128, DOI 10.1016/j.jphotobiol.2005.12.014 . - ISSN 1011-1344
Кл.слова (ненормированные):
ATP -- Bioluminescence -- Earthworms -- Ions -- Luciferin-luciferase systems -- Triton X-100 -- adenosine triphosphate -- anion -- bromine -- calcium ion -- carbonic acid -- cation -- chloride -- chromium derivative -- detergent -- dodecyl sulfate sodium -- inorganic salt -- iodine -- iron derivative -- luciferase -- luciferin -- magnesium ion -- manganese -- nitrate -- phosphate -- sulfate -- sulfite -- triton x 100 -- annelid worm -- article -- bioluminescence -- concentration (parameters) -- controlled study -- enzyme activation -- enzyme activity -- enzyme inhibition -- enzyme mechanism -- in vitro study -- nonhuman -- priority journal -- qualitative analysis -- quantitative analysis -- Adenosine Triphosphate -- Animals -- Cations, Divalent -- Cations, Monovalent -- Detergents -- Firefly Luciferin -- Kinetics -- Luciferases -- Luminescence -- Metals -- Oligochaeta -- Photobiology -- Salts -- Annelida -- Clitellata -- earthworms (sp.) -- Enchytraeidae -- Fridericia heliota -- Oligochaeta (Metazoa) -- Pheretima sieboldi
Аннотация: The study addresses the effect produced by different inorganic salts and detergents (SDS, Triton X-100, the Tween series) on the ATP-dependent bioluminescent reaction catalyzed by the luciferase of the new earthworm species Fridericia heliota (Annelida: Clitellata: Oligochaeta: Enchytraeidae). It has been shown that the effect of divalent metal salts on luminescence is determined by the action of cations. Three of them - Mg2+, Mn2+ and Ca2+ - can stimulate luciferase activity at concentrations varying within a wide range, and Mn2+ can act as a 100%-effective substitute for Mg2+ in F. heliota luminescence reaction in vitro. The inhibitory effect of monovalent metal salts on luminescence is largely determined by the action of the anion part of the molecule. The effectiveness of the inhibitory effect of anions increases in the following order: {Mathematical expression}. Of the sodium salts, dodecyl sulfate, which is an anionic detergent, produces the strongest inhibitory effect on luciferase. On the contrary, nonionic detergents produce a stimulatory effect on the F. heliota luciferase. The action of the most effective of them - Triton X-100 - is determined by its ability to reduce the actual concentration of lipid inhibitors in the reaction mixture. В© 2006 Elsevier B.V. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Rodionova, N.S.; Petushkov, V.N.

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13.

Ca(2+)-activator of the luminescence system of the earthworms Henlea sp., (Annelida: Clitellata: Oligochaeta: Enchytraeidae) / N. S. Rodionova, V. S. Bondar, V. N. Petushkov // Doklady. Biochemistry and biophysics. - 2002. - Vol. 386. - P260-263 . - ISSN 1607-6729
Кл.слова (ненормированные):
calcium -- divalent cation -- edetic acid -- luciferase -- luciferin -- metal -- animal -- annelid worm -- article -- chemistry -- dose response -- enzymology -- genetics -- kinetics -- luminescence -- metabolism -- Animals -- Calcium -- Cations, Divalent -- Dose-Response Relationship, Drug -- Edetic Acid -- Firefly Luciferin -- Kinetics -- Luciferases -- Luminescent Measurements -- Metals -- Oligochaeta

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Rodionova, N.S.; Bondar, V.S.; Petushkov, V.N.

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14.

Experimental estimation of the possible use of submersed macrophytes for biotesting bottom sediments of the Yenisei River [Text] / T. A. Zotina [et al.] // Contemp. Probl. Ecol. - 2014. - Vol. 7, Is. 4. - P410-421, DOI 10.1134/S1995425514040131. - Cited References: 23. - We thank the staff of the Radioecology Laboratory and the Analytical Laboratory, Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, for gamma ray spectrometric and chemical analysis of the samples. This study is supported in part by the Russian Foundation for Basic Research, project no. 14-14-00076, and by "Biological Diversity" program no. 30.5 of the Presidium of the Russian Academy of Sciences. . - ISSN 1995-4255. - ISSN 1995-4263

РУБ Ecology
Рубрики:
MYRIOPHYLLUM-SPICATUM
   TOXICITY
   SENSITIVITY
   GROWTH
   EXPOSURE
   ATRAZINE
   QUALITY
   WATERS
   TESTS
Кл.слова (ненормированные):
Elodea canadensis -- Myriophyllum spicatum -- bottom sediments -- toxicity -- technogenic radionuclides -- heavy metals -- biogens
Аннотация: The laboratory testing of bottom sediments (BSs) from the Yenisei River containing different concentrations of technogenic radionuclides, heavy metals, and biogenic elements (N and P) based on aquatic such plants as Elodea canadensis (Canadian waterweed) and Myriophyllum spicatum (Eurasian watermilfoil) has revealed a higher sensitivity of roots to the general quality of BSs than shoots: shoot length (9%) < root length (11%) < root number (15%) in M. spicatum; shoot length (22%) < root length (42%) < root number (44%) in E. canadensis. In contrast to M. spicatum, the growth parameters of roots and shoots in E. canadensis have differed in a significant statistical manner between most BS samples. A reverse correlation has been found between the increase in shoot length and the activity of technogenic radionuclides in BSs, which is mostly significant in E. canadensis (r (2) = 0.90-0.95, p = 0.05). Since the growth of shoots and roots in E. canadensis has turned out to be more sensitive to changes in the quality of BSs than that in M. spicatum, E. canadensis can be considered more prospective for biotesting BSs.

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Держатели документа:
[Zotina, T. A.
Trofimova, E. A.
Bolsunovsky, A. Ya
Anishenko, O. V.] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zotina, T.A.; Trofimova, E.A.; Bolsunovsky, A.Y.; Anishenko, O.V.; Russian Foundation for Basic Research [14-14-00076]; "Biological Diversity" program of the Presidium of the Russian Academy of Sciences [30.5]

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15.

Cytogenetic abnormalities in aquatic plant Elodea canadensis in anthropogenic contamination zone of Yenisei River [Text] / M. Y. Medvedeva, A. Y. Bolsunovsky, T. A. Zotina // Contemp. Probl. Ecol. - 2014. - Vol. 7, Is. 4. - P422-432, DOI 10.1134/S1995425514040088. - Cited References: 19. - The authors thank their colleagues from the Radioecology laboratory and Analytical laboratory for gamma spectrometric and chemical analyses. The authors express their appreciation to O.V. Kvitko (Institute of Forest, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk) for her valuable methodical consultations. This research is partially funded by both a RFFI-r_Siberia-a, grant no. 13-04-98004 from the Russian Foundation for Basic Research and project no. 30.5 of the Biological Diversity Program of the Presidium of the Russian Academy of Sciences. . - ISSN 1995-4255. - ISSN 1995-4263

РУБ Ecology
Рубрики:
ALLIUM-TEST
GENOTOXICITY
TOXICITY
Кл.слова (ненормированные):
Elodea canadensis -- bottom sediments -- chromosome abnormalities -- anthropogenic radionuclides -- heavy metals -- genotoxicity
Аннотация: Chromosome abnormalities in ana-telophase cells of apical root meristem of aquatic plant Elodea. canadensis (elodea), sampled in 2011-2012 in the Yenisei River at a site with background level of contamination and at several sites on the stretch contaminated with artificial radionuclides, and with chemical pollutants from municipal and industrial discharges of the Krasnoyarsk city. Lowest rate (5.2%) of cells with chromosome abnormalities was registered at sampling site with background level of contamination upstream of the Krasnoyarsk, highest rate of cells with abnormalities (39.7%)-in roots of elodea sampled in bottom sediments with highest concentration of Cs-137. Sum of rates of cells with abnormalities and rates of cells with all types of abnormalities positively correlated with total concentration of artificial and natural radionuclides, with concentration of artificial radionuclides and Cs-137 in bottom sediments of the Yenisei River (r (2) = 0.91-0.96, p < 0.0005 for sum of rates of cells with abnormalities; r (2) = 0.58-0.92, p < 0.05 for all types of abnormalities).

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Держатели документа:
[Medvedeva, M. Yu
Bolsunovsky, A. Ya
Zotina, T. A.] Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Medvedeva, M.Y.; Bolsunovsky, A.Y.; Zotina, T.A.; RFFI-r_Siberia-a from the Russian Foundation for Basic Research [13-04-98004]; Biological Diversity Program of the Presidium of the Russian Academy of Sciences [30.5]

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16.

Bioaccumulation, inter-organ distribution, and retention of waterborne and dietary Am-241 in silver crucian carp [Text] / T. A. Zotina [et al.] // Toxicol Environ. Chem. - 2014. - Vol. 96, Is. 2. - P243-254, DOI 10.1080/02772248.2014.941368. - Cited References: 25 . - ISSN 0277-2248. - ISSN 1029-0486

РУБ Environmental Sciences + Toxicology
Рубрики:
YENISEI RIVER
   TELEOST FISH
   RADIONUCLIDES
   METALS
   AMERICIUM
   EXPOSURE
   ELEMENTS
   ASSIMILATION
   ELASMOBRANCH
   DEPURATION
Кл.слова (ненормированные):
Americium-241 -- assimilation -- Carassius auratus gibelio -- force-feeding
Аннотация: The bioaccumulation of actinide Am-241 from food and water in bodies of freshwater fish (Carassius auratus gibelio, silver crucian carp) has been investigated in laboratory experiments. Homogenized biomass of submerged macrophytes labeled with Am-241 was injected into the fish gullet. Internal organs and tissues of crucian carp could be ranked according to activity concentration of dietary Am-241 after depuration of the digestive tract as follows: liver > gonads > bones > muscles. Accumulation of waterborne Am-241 in internal organs and tissues of crucian carp mainly occurred via the digestive tract. While the concentration of Am-241 in liver of crucian carps decreased during depuration time, its concentration in bones increased indicating slower transfer of Am-241 to skeleton compared to muscles and liver. The retention of dietary Am-241 in the bodies of crucian carp reached 35%-46% of ingested Am-241: 20%-31% was retained in liver, 0.6%-0.8% in skeleton, and 1.4%-2.0% in muscles. The concentration factor of Am-241 from water was 0.4 for the whole body, 0.3 for liver, 0.01 for muscles, and 0.01 for skeleton. Trace amounts of Am-241 were recorded in viscera and muscles of a wild population of silver crucian carp inhabiting a radioactively contaminated part of the Yenisei River.

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Scopus
Держатели документа:
[Zotina, T. A.
Trofimova, E. A.
Dementyev, D. V.
Bolsunovsky, A. Y.] Inst Biophys SB RAS, Krasnoyarsk, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zotina, T.A.; Trofimova, E.A.; Dementyev, D.V.; Bolsunovsky, A.Y.

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17.

Content of metals in compartments of ecosystem of a Siberian pond [Text] / M. I. Gladyshev [et al.] // Arch. Environ. Contam. Toxicol. - 2001. - Vol. 41, Is. 2. - P. 157-162. - Cited References: 26 . - ISSN 0090-4341

РУБ Environmental Sciences + Toxicology
Рубрики:
CHIRONOMUS-RIPARIUS MEIGEN
   NELSON RIVER SYSTEM
   AQUATIC MACROPHYTES
   LARVAE DIPTERA
   HEAVY-METAL
   COPPER
   LEAD
   SEDIMENTS
   CADMIUM
   MANITOBA
Аннотация: During three field seasons (June-September) of 1997-99 contents of Na, K, Ca, Mg, Fe, Mn, Zn, Cu, Al, Cr, Ni, Cd, and Pb were determined in compartments of ecosystem (surrounding soils, bottom sediments, water, zoobenthos, macrophytes, and fish) of a fish and recreation pond situated at the edge of Krasnoyarsk City (Siberia, Russia). Contents of most parts of metals in soils, water, and macrophytes significantly correlated with each other. As concluded, their contents were determined by natural, general, geochemical peculiarities of the region. Heavy metals, contents of which were higher than federal upper limits of concentration, were revealed. In muscles of fish with different feeding spectra-crucian and perch-concentrations of some metals differed significantly; correlation graphs for metals also had different structures. Comparison of our data with those on diverse aquatic ecosystems of Siberia, Europe, North America, and China published in the last decade was carried out. It was concluded that a distribution of heavy metals in the compartments of an aquatic ecosystem presently have to be determined for each particular water body until general regularities are discovered.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
Krasnoyarsk State Agr Univ, Krasnoyarsk 660049, Russia
Krasnoyarsk State Univ, Krasnoyarsk 660042, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Gribovskaya, I.V.; Moskvicheva, A.V.; Muchkina, E.Y.; Chuprov, S.M.; Ivanova, E.A.

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18.

The influence of Ir and Pt addition on the synthesis of fullerenes at atmospheric pressure [Text] / G. N. Churilov [et al.] // Fuller. Nanotub. Carbon Nanostruct. - 2003. - Vol. 11, Is. 4. - P. 371-382, DOI 10.1081/FST-120025856. - Cited References: 17 . - ISSN 1536-383X

РУБ Chemistry, Physical + Nanoscience & Nanotechnology + Materials Science, Multidisciplinary + Physics, Atomic, Molecular & Chemical
Рубрики:
CARBON PLASMA
   STRIATIONS
   DISCHARGE
   FLOW
Кл.слова (ненормированные):
fullerene synthesis -- plasma -- atmospheric pressure -- fullerene derivatives -- iridium -- platinum
Аннотация: The addition of metallic Ir and Pt to a fullerene-forming, atmospheric-pressure plasma reactor was found to influence the generation of carbonaceous products. It was observed that the added metals were efficiently dispersed into the plasma and that their presence increased the yield of fullerenes. The addition of Ir led to a noticeable shift in the fullerene distribution towards C-60, whereas the addition of Pt increased the proportion Of C-60 oxides and decreased the proportion of higher fullerenes. Addition of Ir also caused a reduction of the soot particle size and the formation of a considerable quantity of carbon nanotubes.

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Держатели документа:
Russian Acad Sci, Siberian Branch, LV Kirensky Phys Inst, Krasnoyarsk 660036, Russia
Krasnoyarsk State Tech Univ, Krasnoyarsk, Russia
Rice Univ, Dept Chem, Houston, TX 77251 USA
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
Russian Acad Sci, Siberian Branch, Inst Chem & Chem Technol, Krasnoyarsk 660036, Russia
ИФ СО РАН
ИБФ СО РАН
ИХХТ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Churilov, G.N.; Weisman, R.B.; Bulina, N.V.; Vnukova, N.G.; Puzir, A.P.; Solovyov, L.A.; Bachilo, S.M.; Tsyboulski, D.A.; Glushenko, G.A.

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19.

Pollutant toxicity and detoxification by humic substances: mechanisms and quantitative assessment via luminescent biomonitoring [Text] / N. S. Kudryasheva, A. S. Tarasova // Environ. Sci. Pollut. Res. - 2015. - Vol. 22, Is. 1. - P155-167, DOI 10.1007/s11356-014-3459-6. - Cited References:120. - The work was supported by the Russian Foundation for Basic Research,Grant No. 13-04-98072-sibir-a. Part of the work (analysis ofdetoxification of radioactive solutions) was supported by the RussianScience Foundation, Grant No. 14-14-00076. . - ISSN 0944-1344. - ISSN 1614-7499

РУБ Environmental Sciences
Рубрики:
PHOTOBACTERIUM-LEIOGNATHI LUCIFERASE
DISSOLVED ORGANIC-MATTER
Кл.слова (ненормированные):
Detoxification mechanisms -- Humic substances -- Pollutants -- Bioassays -- Bioluminescence
Аннотация: The paper considers mechanisms of detoxification of pollutant solutions by water-soluble humic substances (HSs), natural detoxifying agents. The problems and perspectives of bioassay application for toxicity monitoring of complex solutions are discussed from ecological point of view. Bioluminescence assays based on marine bacteria and their enzymes are of special attention here; they were shown to be convenient tools to study the detoxifying effects on cellular and biochemical levels. The advantages of bioluminescent enzymatic assay for monitoring both integral and oxidative toxicities in complex solutions of model pollutants and HS were demonstrated. The efficiencies of detoxification of the solutions of organic oxidizers and salts of metals (including radioactive ones) by HS were analyzed. The dependencies of detoxification efficiency on time of exposure to HS and HS concentrations were demonstrated. Antioxidant properties of HS were considered in detail. The detoxifying effects of HS were shown to be complex and regarded as 'external' (binding and redox processes in solutions outside the organisms) and/or 'internal' organismal processes. The paper demonstrates that the HS can stimulate a protective response of bacterial cells as a result of (1) changes of rates of biochemical reactions and (2) stabilization of mucous layers outside the cell walls. Acceleration of auto-oxidation of NADH, endogenous reducer, by HS was suggested as a reason for toxicity increase in the presence of HS due to abatement of reduction ability of intracellular media.

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Держатели документа:
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
RAS, Inst Biophys, SB, Krasnoyarsk 660036, Russia.
ИБФ СО РАН

Доп.точки доступа:
Kudryasheva, N.S.; Tarasova, A.S.; Russian Foundation for Basic Research [13-04-98072-sibir-a]; RussianScience Foundation [14-14-00076]

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20.

Assessment of the Volatility and Thermal Stability of Chemicals that Stimulate Females of Moina macrocopa (Cladocera) to Produce Diapausing Eggs [Text] / T. S. Lopatina, E. S. Zadereev // Russ. J. Ecol. - 2015. - Vol. 46, Is. 1. - P103-108, DOI 10.1134/S1067413615010105. - Cited References:25 . - ISSN 1067-4136. - ISSN 1608-3334

РУБ Ecology
Рубрики:
SEXUAL REPRODUCTION
   INDUCTION
   DAPHNIA
   METABOLITES
   CRUSTACEA
Кл.слова (ненормированные):
cladocerans -- diapause -- chemical communication -- chemical cues
Аннотация: The volatility and thermal stability of chemicals that are contained in the metabolic products of Moina macrocopa females that stimulate the production of diapausing eggs in these females have been studied in experiments with separately cultivated individuals. It has been shown that the gametogenesis-stimulating chemicals in this species are nonvolatile compounds: the effect of the medium that contains the metabolic products of the organism was not decreased after many hours of blowing with argon. Prolonged heating up to 80 degrees C also did not deactivate the ability of these products to change the reproductive mode. The effect of the medium that contains these products decreased after boiling, but if the possibilities of their oxidation or binding with salts of metals were excluded, they retained the ability to change the reproductive mode of the animals even after boiling.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
ИБФ СО РАН

Доп.точки доступа:
Lopatina, T.S.; Zadereev, E.S.

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