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Growth of dominant zooplankton species feeding on plankton microflora in Lake Shira / T. A. Temerova, A. P. Tolomeyev, A. G. Degermendzhy // Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - P235-243, DOI 10.1023/A:1015607304508 . - ISSN 1386-2588
Кл.слова (ненормированные):
Arctodiaptomus salinus -- Brachionus plicatilis -- Life history -- Reproduction -- Specific growth rate -- diet -- food limitation -- growth rate -- life history -- reproduction -- saline lake -- zooplankton -- Russian Federation -- algae -- Arctodiaptomus -- Bacteria (microorganisms) -- Brachionus -- Brachionus plicatilis -- Calanoida -- Copepoda -- Cyanobacteria -- Invertebrata -- Rotifera
Аннотация: Batch cultures and continuous flow cultures were used to study the growth rates of zooplankton species from Shira lake, the rotifer Brachionus plicatilis Muller and calanoid copepod Arctodiaptomus salinus Daday, which were fed on phytoplankton and bacterioplankton from the lake. Analyses of the birth and survival rates were used to demonstrate that the lake phytoplankton, consisting mostly of cyanobacteria and diatomaceous algae, is inadequotes for optimal realisation of the reproductive potential of B. plicatilis when compared with the bacterial diet. The study revealed that the kinetic growth characteristics of the two zooplankters were similar: B. plicatilis rmax, 0.120 d-1; S0, 0.253; and Ks, 0.114 mg dry mass l-1; and for A. salinus rmax, 0.129 d-1; S0, 0.240; and Ks, 0.171 mg dry mass l-1. Fluctuations in natural food concentration reduced the growth rate of both species. Even though the threshold concentration of food for B. plicatilis and A. salinus were quite similar, the copepods were less sensitive to food limitation.

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Держатели документа:
Institute of Biophysics of SB RAS, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Temerova, T.A.; Tolomeyev, A.P.; Degermendzhy, A.G.

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Use of human wastes oxidized to different degrees in cultivation of higher plants on the soil-like substrate intended for closed ecosystems / A. A. Tikhomirov [et al.] // Advances in Space Research. - 2010. - Vol. 46, Is. 6. - P744-750, DOI 10.1016/j.asr.2010.02.024 . - ISSN 0273-1177
Кл.слова (ненормированные):
Life support systems -- Microflora -- Mineralized human wastes -- Phototrophic unit -- Wet incineration -- Alternating electromagnetic field -- Bioregenerative life support systems -- Degree of oxidations -- Denitrifying microorganisms -- Growth and development -- Higher plants -- Human waste -- Life support systems -- Mass exchange -- Microbiotas -- Microflora -- Microscopic fungi -- Mineralized human wastes -- Nutrient solution -- Oxidation level -- Phytopathogenic bacteria -- Plant productivity -- Soil-like substrate -- Wheat plants -- Biomolecules -- Electromagnetic fields -- Irrigation -- Magnetic field effects -- Metabolism -- Metabolites -- Oxidation -- Plants (botany) -- Soils -- Solvent extraction -- Wastes -- Waste incineration
Аннотация: To close mass exchange loops in bioregenerative life support systems more efficiently, researchers of the Institute of Biophysics SB RAS (Krasnoyarsk, Russia) have developed a procedure of wet combustion of human wastes and inedible parts of plants using H2O2 in alternating electromagnetic field. Human wastes pretreated in this way can be used as nutrient solutions to grow plants in the phototrophic unit of the LSS. The purpose of this study was to explore the possibilities of using human wastes oxidized to different degrees to grow plants cultivated on the soil-like substrate (SLS). The treated human wastes were analyzed to test their sterility. Then we investigated the effects produced by human wastes oxidized to different degrees on growth and development of wheat plants and on the composition of microflora in the SLS. The irrigation solution contained water, substances extracted from the substrate, and certain amounts of the mineralized human wastes. The experiments showed that the human wastes oxidized using reduced amounts of 30% H2O2: 1 ml/g of feces and 0.25 ml/ml of urine were still sterile. The experiments with wheat plants grown on the SLS and irrigated by the solution containing treated human wastes in the amount simulating 1/6 of the daily diet of a human showed that the degree of oxidation of human wastes did not significantly affect plant productivity. On the other hand, the composition of the microbiota of irrigation solutions was affected by the oxidation level of the added metabolites. In the solutions supplemented with partially oxidized metabolites yeast-like microscopic fungi were 20 times more abundant than in the solutions containing fully oxidized metabolites. Moreover, in the solutions containing incompletely oxidized human wastes the amounts of phytopathogenic bacteria and denitrifying microorganisms were larger. Thus, insufficiently oxidized sterile human wastes added to the irrigation solutions significantly affect the composition of the microbiological component of these solutions, which can ultimately unbalance the system as a whole. В© 2010 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, Akademgorodok, 50/50, Krasnoyarsk 660036, Russian Federation
Universite Blaise Pascal, 24 avenue des Landais, 63174 Aubiere cedex, France
ESA/ESTEC, Keplerlaan 1, 2201 AZ Noordwijk, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Kudenko, Y.A.; Ushakova, S.A.; Tirranen, L.S.; Gribovskaya, I.A.; Gros, J.-B.; Lasseur, C.

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Testing soil-like substrate for growing plants in bioregenerative life support systems / J. B. Gros [et al.] // Advances in Space Research. - 2005. - Vol. 36, Is. 7. - P1312-1318, DOI 10.1016/j.asr.2005.05.079 . - ISSN 0273-1177
Кл.слова (ненормированные):
Life support system -- Matter recycling -- Plants -- Soil-like substrate -- Biodiversity -- Correlation methods -- Growth kinetics -- Hormones -- Plants (botany) -- Recycling -- Soils -- Bioregeneration -- Life support system -- Matter recycling -- Soil-like substrate -- Space research
Аннотация: We studied soil-like substrate (SLS) as a potential candidate for plant cultivation in bioregenerative life support systems (BLSS). The SLS was obtained by successive conversion of wheat straw by oyster mushrooms and worms. Mature SLS contained 9.5% humic acids and 4.9% fulvic acids. First, it was shown that wheat, bean and cucumber yields as well as radish yields when cultivated on mature SLS were comparable to yields obtained on a neutral substrate (expanded clay aggregate) under hydroponics. Second, the possibility of increasing wheat and radish yields on the SLS was assessed at three levels of light intensity: 690, 920 and 1150 ?mol m-2 s-1 of photosynthetically active radiation (PAR). The highest wheat yield was obtained at 920 ?mol m-2 s-1, while radish yield increased steadily with increasing light intensity. Third, long-term SLS fertility was tested in a BLSS model with mineral and organic matter recycling. Eight cycles of wheat and 13 cycles of radish cultivation were carried out on the SLS in the experimental system. Correlation coefficients between SLS nitrogen content and total wheat biomass and grain yield were 0.92 and 0.97, respectively, and correlation coefficients between nitrogen content and total radish biomass and edible root yield were 0.88 and 0.87, respectively. Changes in hormone content (auxins, gibberellins, cytokinins and abscisic acid) in the SLS during matter recycling did not reduce plant productivity. Quantitative and species compositions of the SLS and irrigation water microflora were also investigated. Microbial community analysis of the SLS showed bacteria from Bacillus, Pseudomonas, Proteus, Nocardia, Mycobacterium, Arthrobacter and Enterobacter genera, and fungi from Trichoderma, Penicillium, Fusarium, Aspergillus, Mucor, Botrytis, and Cladosporium genera. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
LGCB, Universite B. Pascal, CUST, BP206, 63174 Aubiere cedex, France
Environmental Control and Life Support Section, ESA-Estec, Postbus 299, 2200 AG, Noordwijk, Netherlands
Institute of Biophysics (Russian Academy of Sciences, Siberian Branch), Academgorodok, Krasnoyarsk 660036, Russian Federation
Department of Plant Physiology and Biotechnology, Tomsk State University, Lenin av. 36, Tomsk 634050, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gros, J.B.; Lasseur, Ch.; Tikhomirov, A.A.; Manukovsky, N.S.; Kovalev, V.S.; Ushakova, S.A.; Zolotukhin, I.G.; Tirranen, L.S.; Karnachuk, R.A.; Dorofeev, V.Yu.

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Biological and physicochemical methods for utilization of plant wastes and human exometabolites for increasing internal cycling and closure of life support systems / I. G. Zolotukhin [et al.] // Advances in Space Research. - 2005. - Vol. 35, Is. 9 SPEC. ISS. - P1559-1562, DOI 10.1016/j.asr.2005.01.006 . - ISSN 0273-1177
Кл.слова (ненормированные):
BLSS -- Desalting -- Higher plants -- NaCl utilization -- SLS -- Biomass -- Crops -- Decomposition -- Electrodialysis -- Harvesting -- Metabolites -- Soils -- Wastes -- BLSS -- Higher plants -- NaCl utilization -- SLS -- Plants (botany) -- Biomass -- Decay -- Deionization -- Harvesting -- Plants -- Soil -- Wastes -- Wheat -- sodium chloride -- article -- biomass -- bioremediation -- culture medium -- feces -- growth, development and aging -- human -- metabolism -- methodology -- microbiology -- microclimate -- urine -- waste management -- wheat -- Biodegradation, Environmental -- Biomass -- Culture Media -- Ecological Systems, Closed -- Feces -- Humans -- Life Support Systems -- Sodium Chloride -- Soil Microbiology -- Triticum -- Urine -- Waste Management
Аннотация: Wheat was cultivated on soil-like substrate (SLS) produced by the action of worms and microflora from the inedible biomass of wheat. After the growth of the wheat crop, the inedible biomass was restored in SLS and exposed to decomposition ("biological" combustion) and its mineral compounds were assimilated by plants. Grain was returned to the SLS in the amount equivalent to human solid waste produced by consumption of the grain. Human wastes (urine and feces) after physicochemical processing turned into mineralized form (mineralized urine and mineralized feces) and entered the plants' nutrient solution amounts equal to average daily production. Periodically (once every 60-70 days) the nutrient solution was partly (up to 50%) desalinated by electrodialysis. Due to this NaCl concentration in the nutrient solution was sustained at a fixed level of about 0.26%. The salt concentrate obtained could be used in the human nutrition through NaCl extraction and the residuary elements were returned through the mineralized human liquid wastes into matter turnover. The control wheat cultivation was carried out on peat with use of the Knop nutrient solution. Serial cultivation of several wheat vegetations within 280 days was conducted during the experiment. Grain output varied and yield/harvest depended, in large part, upon the amount of inedible biomass returned to SLS and the speed of its decomposition. After achieving a stationary regime, (when the quantity of wheat inedible biomass utilized during vegetation in SLS is equal to the quantity of biomass introduced into SLS before vegetation) grain harvest in comparison with the control was at most 30% less, and in some cases was comparable to the control harvest values. The investigations carried out on the wheat example demonstrated in principle the possibility of long-term functioning of the LSS photosynthesizing link based on optimizations of biological and physicochemical methods of utilization of the human and plants wastes. The possibilities for the use of these technologies for the creation integrated biological-physicochemical LSS with high closure degree of internal matter turnover are discussed in this paper. В© 2005 Published by Elsevier Ltd on behalf of COSPAR.

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Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zolotukhin, I.G.; Tikhomirov, A.A.; Kudenko, Yu.A.; Gribovskaya, I.V.

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Wheat growth on neutral and soil-like substrates: Carbon dioxide exchange and microflora / J. -B. Gros [et al.] // Acta Horticulturae. - 2004. - Vol. 644. - P243-248
Кл.слова (ненормированные):
Carbon dioxide -- Closed ecological system
Аннотация: The soil-like substrate (SLS), which is a potential candidate for employment in closed ecological systems, has been tested. CO2 exchange, composition and numbers of soil microflora have been examined in .wheat-SLS. system. The results produced have been compared to analogous characteristics of .wheat-neutral substrate. system. A hydroponic method was used under wheat growing on the neutral substrate (expanded clay aggregate). Plants for both studies were grown in closed environment from seed to physiological maturity. In the .wheat-SLS. system, the net photosynthetic rate of canopy was positive in the course of 6.55 days after planting. The net photosynthetic rate of canopy in .wheat-neutral substrate. system was positive in the entire course of vegetation. According to calculations in the course of vegetation, photosynthesis has withdrawn 3.28 kg m-2 CO2 from the .wheat-SLS. system and 3.40 kg m-2 CO2 from the .wheat-neutral substrate. system. On the SLS dominant among bacteria were the spore-forming bacteria from Bacillus genus, among fungi . from Trichoderma genus. In the hydroponic cultivation on neutral substrate dominant were bacteria from Pseudomonas genus, most commonly found fungi were species from Fusarium and Botrytis genera.

Scopus
Держатели документа:
LGCB, Universte B. Pascal, BP206, FR-63174 Aubiere cedex, France
ESA, Estec 2200 AG Noordwijk, Netherlands
Academgorodok, Institute of Biophysics, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gros, J.-B.; Lasseur, C.; Tikhomirov, A.A.; Manukovsky, N.S.; Ushakova, S.A.; Zolotukhin, I.G.; Tirranen, L.S.; Borodina, E.V.; Kovalev, V.S.

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Synthesis of biomass and utilization of plants wastes in a physical model of biological life-support system / A. A. Tikhomirov [et al.] // Acta Astronautica. - 2003. - Vol. 53, Is. 4-10. - P249-257, DOI 10.1016/S0094-5765(03)00137-1 . - ISSN 0094-5765
Кл.слова (ненормированные):
Ecosystems -- Microorganisms -- pH -- Photosynthesis -- Plants (botany) -- Synthesis (chemical) -- Waste utilization -- Biological life support systems (BLLS) -- Gas exchange -- Plant respiration -- Biomass -- carbon dioxide -- Agaricales -- article -- biomass -- bioremediation -- growth, development and aging -- hydroponics -- incineration -- metabolism -- methodology -- microbiology -- microclimate -- photosynthesis -- plant physiology -- radish -- space flight -- waste management -- weightlessness -- wheat -- Agaricales -- Biodegradation, Environmental -- Biomass -- Carbon Dioxide -- Ecological Systems, Closed -- Environmental Microbiology -- Hydroponics -- Incineration -- Life Support Systems -- Photosynthesis -- Plant Physiology -- Raphanus -- Space Flight -- Triticum -- Waste Management -- Weightlessness
Аннотация: The paper considers problems of biosynthesis of higher plants' biomass and "biological incineration" of plant wastes in a working physical model of biological LSS. The plant wastes are "biologically incinerated" in a special heterotrophic block involving Califomian worms, mushrooms and straw. The block processes plant wastes (straw, haulms) to produce soil-like substrate (SLS) on which plants (wheat, radish) are grown. Gas exchange in such a system consists of respiratory gas exchange of SLS and photosynthesis and respiration of plants. Specifics of gas exchange dynamics of high plants - SLS complex has been considered. Relationship between such a gas exchange and PAR irradiance and age of plants has been established. Nitrogen and iron were found to the first to limit plants' growth on SLS when process conditions are deranged. The SLS microflora has been found to have different kinds of ammonifying and denitrifying bacteria which is indicative of intensive transformation of nitrogen-containing compounds. The number of physiological groups of microorganisms in SLS was, on the whole, steady. As a result, organic substances - products of exchange of plants and microorganisms were not accumulated in the medium, but mineralized and assimilated by the biocenosis. Experiments showed that the developed model of a man-made ecosystem realized complete utilization of plant wastes and involved them into the intrasystem turnover. В© 2003 International Astronautical Federation. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russian Federation
Universite B. Pascal, Clermont-Ferrand, France
Environ. Contr. Life Support Sect., ESA, Estec Noonvijk, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Ushakova, S.A.; Manukovsky, N.S.; Lisovsky, G.M.; Kudenko, Yu.A.; Koyalev, V.S.; Gribovskaya, I.V.; Tirranen, L.S.; Zolotukhin, I.G.; Gros, J.B.; Lasseur, Ch.

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Light intensity and production parameters of phytocenoses cultivated on soil-like substrate under controled environment conditions / A. A. Tikhomirov [et al.] // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1775-1780, DOI 10.1016/S0273-1177(03)80020-0 . - ISSN 0273-1177
Кл.слова (ненормированные):
Bacteria -- Biology -- Crops -- Photosynthesis -- Substrates -- Light intensity -- Space research -- biosphere -- Biomass -- Carbon Dioxide -- Colony Count, Microbial -- Culture Media -- Ecological Systems, Closed -- Enterobacteriaceae -- Life Support Systems -- Light -- Photosynthesis -- Plant Transpiration -- Raphanus -- Soil Microbiology -- Triticum -- Water Microbiology
Аннотация: To increase the degree of closure of biological life support systems of a new generation, we used vermicomposting to involve inedible phytomass in the intra-system mass exchange. The resulting product was a soil-like substrate, which was quite suitable for growing plants (Manukovsky et al. 1996, 1997). However, the soil-like substrate can be regarded as a candidate for inclusion in a system only after a comprehensive examination of its physical, chemical, and other characteristics. An important criterion is the ability of the soil-like substrate to supply the necessary mineral elements to the photosynthesizing component under the chosen cultivation conditions. Thus, the purpose of this work was to study the feasibility of enhancing the production activity of wheat and radish crops by varying the intensity of photosynthetically active radiation, without decreasing the harvest index. The increase of light intensity from 920 to 1150 ?molВ·m -2В·s -1 decreased the intensity of apparent photosynthesis of the wheat crops and slightly increased the apparent photosynthesis of the radish crops The maximum total and grain productivity (kg/m 2) of the wheat crops was attained at the irradiance of 920 ?molВ·m -2В·s -1. Light intensity of 1150 ?molВ·m -2В·s -1 decreased the productivity of wheat plants and had no significant effect on the productivity of the radish crops (kg/m 2) as compared to 920 ?molВ·m 2В·s -1. The qualitative and quantitative composition of microflora of the watering solution and substrate was determined by the condition of plants, developmental phase and light intensity. By the end of wheat growth under 1150 ?molВ·m -2В·s -1 the numbers of bacteria of the coliform family and phytopathogenic bacteria in the watering solution and substrate were an order of magnitude larger than under other illumination conditions. The obtained data suggest that the cultivation of plants in a life support system on soil-like substrate from composts has a number of advantages over the cultivation on neutral substrates, which require continual replenishment of the plant nutrient solution from the system's store to complement the macro- and microelements. Yet, a number of problems arise, including those related to the controlling of the production activity of the plants by the intensity of photosynthetically active radiation. It is essential to understand why the intensity of production processes is limited at higher irradiation levels and to overcome the factors responsible for this, so that the soil-like substrate could have an even better chance in the competition for the best plant cultivation technology to be used in biological life support systems. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, RAS SB, Krasnoyarsk 660036, Russian Federation
Tomsk State University, Lenin street, 36, Tomsk 634050, Russian Federation
LGCB, Universte B. Pascal, BP206, Aubiere cedex 63174, France
ESA/ESTEC, Noordwijk, AG 2200, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Ushakova, S.A.; Gribovskaya, I.A.; Tirranen, L.S.; Manukovsky, N.S.; Zolotukhin, I.G.; Karnachuk, R.A.; Gros, J.-B.; Lasseur, Ch.

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Mass exchange in an experimental new-generation life support system model based on biological regeneration of environment / A. A. Tikhomirov [et al.] // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1711-1720, DOI 10.1016/S0273-1177(03)80017-0 . - ISSN 0273-1177
Кл.слова (ненормированные):
Biomass -- Photosynthesis -- Plants (botany) -- Transpiration -- Mass exchange -- Life support systems (spacecraft) -- ammonia -- nitrogen -- oxygen -- biosphere -- animal -- annelid worm -- article -- biological model -- biomass -- bioremediation -- evaluation -- growth, development and aging -- human -- metabolism -- microclimate -- photosynthesis -- Pleurotus -- radish -- wheat -- Ammonia -- Animals -- Biodegradation, Environmental -- Biomass -- Ecological Systems, Closed -- Evaluation Studies -- Humans -- Life Support Systems -- Models, Biological -- Nitrogen -- Oligochaeta -- Oxygen -- Photosynthesis -- Pleurotus -- Raphanus -- Triticum
Аннотация: An experimental model of a biological life support system was used to evaluate qualitative and quantitative parameters of the internal mass exchange. The photosynthesizing unit included the higher plant component (wheat and radish), and the heterotrophic unit consisted of a soil-like substrate, California worms, mushrooms and microbial microflora. The gas mass exchange involved evolution of oxygen by the photosynthesizing component and its uptake by the heterotroph component along with the formation and maintaining of the SLS structure, growth of mushrooms and California worms, human respiration, and some other processes. Human presence in the system in the form of "virtual human" that at regular intervals took part in the respirative gas exchange during the experiment. Experimental data demonstrated good oxygen/carbon dioxide balance, and the closure of the cycles of these gases was almost complete. The water cycle was nearly 100% closed. The main components in the water mass exchange were transpiration water and the watering solution with mineral elements. Human consumption of the edible plant biomass (grains and roots) was simulated by processing these products by a unique physicochemical method of oxidizing them to inorganic mineral compounds, which were then returned into the system and fully assimilated by the plants. The oxidation was achieved by "wet combustion" of organic biomass, using hydrogen peroxide following a special procedure, which does not require high temperature and pressure. Hydrogen peroxide is produced from the water inside the system. The closure of the cycle was estimated for individual elements and compounds. Stoichiometric proportions are given for the main components included in the experimental model of the system. Approaches to the mathematical modeling of the cycling processes are discussed, using the data of the experimental model. Nitrogen, as a representative of biogenic elements, shows an almost 100% closure of the cycle inside the system. The proposed experimental model of a biological system is discussed as a candidate for potential application in the investigations aimed at creating ecosystems with largely closed cycles of the internal mass exchange. The formation and maintenance of sustainable cycling of vitally important chemical elements and compounds in biological life support systems (BLSS) is an extremely pressing problem. To attain the stable functioning of biological life support systems (BLSS) and to maintain a high degree of closure of material cycles in them, it is essential to understand the character of mass exchange processes and stoichiometric proportions of the initial and synthesized components of the system. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russian Federation
Universite B. Pascal, Clermont-Ferrand, France
Environ. Control/Life Support Sect., ESA, Estec Noorwijk, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Ushakova, S.A.; Manukovsky, N.S.; Lisovsky, G.M.; Kudenko, Yu.A.; Kovalev, V.S.; Gubanov, V.G.; Barkhatov, Yu.V.; Gribovskaya, I.V.; Zolotukhin, I.G.; Gros, J.B.; Lasseur, Ch.

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Experimental evaluation of the processes resulting from the introduction of the transgenic microorganism Escherichia coli Z905/pPHL7 (lux+) into aquatic microcosms / T. V. Kargatova [et al.] // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1769-1774, DOI 10.1016/S0273-1177(03)00119-4 . - ISSN 0273-1177
Кл.слова (ненормированные):
Escherichia coli -- Genes -- Sensitivity analysis -- Aquatic microcosms -- Space research -- genetically modified organism -- Adaptation, Physiological -- Ampicillin Resistance -- Animals -- Chemiluminescent Measurements -- Colony Count, Microbial -- Daphnia -- Ecological Systems, Closed -- Environmental Monitoring -- Escherichia coli -- Fresh Water -- Gene Expression Regulation, Bacterial -- Organisms, Genetically Modified -- Plasmids -- Protozoa -- Time Factors -- Water Microbiology
Аннотация: The processes resulting from the introduction of the transgenic microorganism (TM) E. coli Z905/pPHL7 into aquatic microcosms have been modeled experimentally. It has been shown that the TM E. coli is able to adapt to a long co-existence with indigenous heterotrophic microflora in variously structured microcosms. In more complex microcosms the numerical dynamics of the introduced E. coli Z905/pPHL7 population is more stable. In the TM populations staying in the microcosms for a prolonged time, changes are recorded in the phenotypic expression of plasmid genes (ampicillin resistance and the luminescence level) and chromosome genes (morphological and physiological traits). However, in our study microcosms, the recombinant plasmid persisted in the TM cells for 6 years after the introduction, and as the population adapts to the conditions of the microcosms, the efficiency of the cloned gene expression in the cells is restored. In the microcosms with high microalgal counts (107 cells/ml), cells with a high threshold of sensitivity to ampicillin dominate in the population of the TM E. coli Z905/pPHL7. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

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Держатели документа:
Institute of Biophysics, SB RAS, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kargatova, T.V.; Boyandin, A.N.; Popova, L.Yu.; Pechurkin, N.S.

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10.

Functional, regulatory and indicator features of microorganisms in man-made ecosystems / L. A. Somova, N. S. Pechurkin // Advances in Space Research. - 2001. - Vol. 27, Is. 9. - P1563-1570, DOI 10.1016/S0273-1177(01)00247-2 . - ISSN 0273-1177
Кл.слова (ненормированные):
Biomass -- Carbon dioxide -- Ecosystems -- Life support systems (spacecraft) -- Photosynthesis -- Sewage treatment -- Soils -- Human microfloras -- Microorganisms -- carbon -- carbon dioxide -- artificial ecosystem -- article -- biomass -- bioreactor -- ecosystem -- human -- intestine -- metabolism -- microbiology -- microclimate -- plant root -- sewage -- wheat -- Biomass -- Bioreactors -- Carbon -- Carbon Dioxide -- Ecological Systems, Closed -- Ecosystem -- Environmental Microbiology -- Humans -- Intestines -- Life Support Systems -- Plant Roots -- Sewage -- Triticum -- Waste Disposal, Fluid
Аннотация: Functional, regulatory and indicator features of microorganisms in development and functioning of the systems and sustaining stability of three man-made ecosystem types has been studied. 1) The functional (metabolic) feature was studied in aquatic ecosystems of biological treatment of sewage waters for the reducer component. 2) The regulatory feature of bacteria for plants (producer component) was studied in simple terrestrial systems "wheat plants-rhizospheric microorganisms - artificial soil" where the behavior of the system varied with activity of the microbial component. For example with atmospheric carbon dioxide content elevated microbes promote intensification of photosynthesis processes, without binding the carbon in the plant biomass. 3) The indicator feature for the humans (consumer component) was studied in Life Support Systems (LSS). High sensitivity of human microflora to system conditions allowed its use as an indicator of the state of both system components and the entire ecosystem. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

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Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Somova, L.A.; Pechurkin, N.S.

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11.

Micro- and macroorganisms in life support systems (problem of coexistence) / L. A. Somova, N. S. Pechurkin, R. C. Huttenbach // Advances in Space Research. - 1996. - Vol. 18, Is. 12. - P259-263, DOI 10.1016/0273-1177(96)00120-2 . - ISSN 0273-1177
Аннотация: Every life support system has an associated microflora that is not essential to functioning of the system. At the same time, the confined space of a closed system increases the significance of the associated microflora causing closer contact between components and enhancing the intensity of exchange between them. For any life support system that is functioning normally, there exists an optimum between the effort necessary to maintain the system in a healthy state and the damage the introduction of alien microflora can cause. Copyright В© 1996 COSPAR.

Scopus
Держатели документа:
Institute of Biophysics, Krasnoyarsk, 660036, Russian Federation
Nelson Space Services Ltd., 90 London Road, London SEI 6LN, United Kingdom : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Somova, L.A.; Pechurkin, N.S.; Huttenbach, R.C.

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12.

Experimental microcosms as models of natural ecosystems for monitoring survival of genetically modified microorganism. / U - Popova LYu [et al.] // Life support & biosphere science : international journal of earth space. - 1999. - Vol. 6, Is. 3. - P193-197 . - ISSN 1069-9422
Кл.слова (ненормированные):
bacterial DNA -- recombinant DNA -- adaptation -- article -- ecosystem -- Escherichia coli -- genetics -- microbiology -- plasmid -- risk assessment -- Adaptation, Biological -- DNA, Bacterial -- DNA, Recombinant -- Ecosystem -- Escherichia coli -- Microbiology -- Plasmids -- Risk Assessment -- Soil Microbiology -- Water Microbiology
Аннотация: An experimental approach for investigation of genetically modified microorganisms (GMMO) introduced into model ecosystems to evaluate potential risk of propagation of recombinant plasmids in surrounding medium has been developed. The object of modeling was Escherichia coli Z905 strain with a recombinant plasmid with bacterial luminescence genes, which was introduced into water microcosms of different structure. The approach involves comprehensive investigation of GMMO at four hierarchical levels: molecular (retaining the structure of the plasmid and expression of cloned genes); cellular (variation of metabolic activity); population (competitive power and metabolic interactions of GMMO with indigenous microflora, migration of recombinant and natural plasmids); ecosystem (effect of GMMO and cloned genes on ecosystem parameters). The experimental evidence and theoretical estimates are intended to form grounds to develop a basic version of an ecological certificate for different GMMO variants.

Scopus
Держатели документа:
Institute of Biophysics (Russian Academy of Sciences, Siberian Branch), Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
, U - Popova LYu; Pechurkin, N.S.; Maksimova, E.E.; Kargatova, T.V.; , U - Krylova TYu; Lobova, T.I.; Boyandin, A.N.

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13.

Catalase activity as a potential indicator of the reducer component of small closed ecosystems / A. B. Sarangova, L. A. Somova, T. I. Pisman // Advances in Space Research. - 1997. - Vol. 20, Is. 10. - P1945-1948 . - ISSN 0273-1177
Кл.слова (ненормированные):
carboxymethylcellulose -- catalase -- animal -- article -- Bacillus -- bacterial count -- Chlorella -- culture medium -- enzymology -- growth, development and aging -- metabolism -- microclimate -- Paramecium -- Animals -- Bacillus -- Carboxymethylcellulose -- Catalase -- Chlorella -- Colony Count, Microbial -- Culture Media -- Ecological Systems, Closed -- Paramecium
Аннотация: Dynamics of catalase activity has been shown to reflect the growth curve of microorganisms in batch cultivation (celluloselythic bacteria Bacillus acidocaldarius and bacteria of the associated microflora Chlorella vulgaris). Gas and substrate closure of the three component ecosystems with spatially separated components "producer-consumer-reducer" (Chl. vulgaris-Paramecium caudatum-B. acidocaldarius, two bacterial strains isolated from the associated microflora Chl. vulgaris) demonstrated that the functioning of the reducer component can be estimated by the catalase activity of microorganisms of this component. В© 1997 COSPAR. Published by Elsevier Science Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sarangova, A.B.; Somova, L.A.; Pisman, T.I.

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14.

Psychrophilic and psychrotolerant heterotrophic microorganisms of Middle Siberian karst cavities / S. V. Khizhnyak [et al.] // Russian Journal of Ecology. - 2003. - Vol. 34, Is. 4. - P231-235, DOI 10.1023/A:1024537513439 . - ISSN 1067-4136
Кл.слова (ненормированные):
Caves -- Heterotrophic microorganisms -- Karst cavities -- Psychorophilic and psychrotolerant microorganisms -- bacterium -- cavernicolous species -- fungus -- heterotrophy -- karst -- low temperature -- Russian Federation -- Arthrobacter -- Bacteria (microorganisms) -- Chrysosporium -- Corynebacteriaceae -- Fungi -- Mucor -- Penicillium -- Pseudomonas
Аннотация: The natural microflora of Middle Siberian karst cavities, which comprises psychrotolerant bacteria and fungi capable of growing at 3-15 and 3...+28В°C, respectively, has been studied. Bacteria are ubiquitous in caves, their count varying from 103 to 107 cells/g ground. The bacteria have been identified as Pseudomonas, Arthrobacter, Bacillus, and coryneform bacteria. Fungi have been found in places exposed to increased anthropogenic impact, their count being as large as 106 to 107 cells/g ground. Mucor, Penicillium, and Chrysosporium were dominant fungal genera.

Scopus
Держатели документа:
Krasnoyarsk State Agrarian Univ., Pr. Mira 88, Krasnoyarsk 660001, Russian Federation
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Khizhnyak, S.V.; Tausheva, I.V.; Berezikova, A.A.; Nesterenko, E.V.; Rogozin, D.Yu.

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15.

ACCUMULATION OF POLYHYDROXYBUTYRATE BY THE AEROBIC CO-OXIDIZING BACTERIUM SELIBERIA-CARBOXYDOHYDROGENA [Text] / T. G. VOLOVA, G. S. KALACHEVA, V. M. KONSTANTINOVA // Microbiology. - 1994. - Vol. 63, Is. 2. - P109-112. - Cited References: 20 . - 4. - ISSN 0026-2617

РУБ Microbiology

Аннотация: The synthesis of polyhydroxybutyrate by the carboxydobacterium Seliberia carboxydohydrogena under various growth conditions was studied. The bacterium was able to accumulate poly-beta-hydroxybutyric acid and a heteropolymer that was a product of copolymerization of hydroxybutyric and hydroxyvaleric acids. Nitrogen and sulfur exerted the strongest effect on polymer accumulation in carboxydobacteria: The maximum polymer concentrations in cells reached 28%. Specimens of polyhydroxybutyrate films were obtained, and their degradability by soil microflora was shown.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
VOLOVA, T.G.; KALACHEVA, G.S.; KONSTANTINOVA, V.M.

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16.

Multiple antibiotic resistance of heterotrophic bacteria in the littoral zone of Lake Shira as an indicator of human impact on the ecosystem / T. I. Lobova [et al.] // Microbiological Research. - 2008. - Vol. 163, Is. 2. - P152-160, DOI 10.1016/j.micres.2006.03.014 . - ISSN 0944-5013
Кл.слова (ненормированные):
Antibiotic resistance -- Aquatic ecosystems -- Heterotrophic bacteria -- Human impact -- Monitoring -- Antibiotics -- Bacteria -- Ecosystems -- Environmental impact -- Mammals -- Antibiotic resistance -- Aquatic ecosystems -- Heterotrophic bacteria -- Materials -- antiinfective agent -- fresh water -- allochthony -- anthropogenic effect -- antibiotic resistance -- bacterium -- concentration (composition) -- heterotrophy -- intertidal environment -- monitoring -- recreational facility -- spring (season) -- summer -- animal -- article -- bacterial count -- bacterium -- drug effect -- ecosystem -- environmental monitoring -- heterotrophy -- human -- isolation and purification -- methodology -- microbiological examination -- microbiology -- multidrug resistance -- Russian Federation -- season -- Animals -- Anti-Bacterial Agents -- Bacteria -- Colony Count, Microbial -- Drug Resistance, Multiple, Bacterial -- Ecosystem -- Environmental Monitoring -- Fresh Water -- Heterotrophic Processes -- Humans -- Microbial Sensitivity Tests -- Russia -- Seasons -- Eurasia -- Khakassia -- Lake Shira -- Russian Federation -- Animalia -- Bacteria (microorganisms)
Аннотация: Resistance to Ampicillin and Kanamycin displayed by heterotrophic bacteria isolated in Summer and in Spring from the littoral and the central parts of Lake Shira (a therapeutic lake in the Khakasia Republic, Russia) has been investigated. It has been found that in Summer, human and animal microflora featuring multiple antibiotic resistance (to Ampicillin and Kanamycin) predominates in all the studied stations of the littoral zone of the lake. In Spring, concentrations of bacteria featuring multiple antibiotic resistance decrease significantly and bacteria sensitive to antibiotics predominate in the lake. Emergence of multiple antibiotic resistance in bacteria of Lake Shira is caused by the input of allochthonous bacteria into the lake; this feature of heterotrophic bacteria of Lake Shira can be used to monitor the impact on the ecosystem made by health resorts. В© 2006 Elsevier GmbH. All rights reserved.

Scopus
Держатели документа:
LTD Territory-oriented information systems, Institute of Computational Modeling, Russian Academy of Sciences, Akademgorodok 50, Krasnoyarsk 660036, Russian Federation
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Akademgorodok 50, 660036, Russian Federation
Center of Hygiene and Epidemiology in Krasnoyarsk Region, Sopochnaya 38, Russian Federation
Krasnoyarsk Scientific Centre, Akademgorodok 50, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Lobova, T.I.; Barkhatov, Y.V.; Salamatina, O.V.; Popova, L.Yu.

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17.

Antibiotic resistance of heterotrophic bacteria in Shira lake: Natural and anthropogenic impacts / T. I. Lobova, Yu. V. Barkhatov, L. Yu. Popova // Aquatic Microbial Ecology. - 2002. - Vol. 30, Is. 1. - P11-18 . - ISSN 0948-3055
Кл.слова (ненормированные):
Anthropogenic load -- Antibiotic resistance -- Brackish lake -- Halotolerance -- Heterotrophic bacteria -- Mathematical modeling -- algae -- Bacteria (microorganisms) -- Chlorophyta
Аннотация: Studies were conducted to investigate the antibiotic resistance of freshwater, halophile and moderate halotolerant bacteria isolated from the nearshore part of Shira lake, which is affected by the activity of a health resort, and from the central part of the lake in the summer (June to August) of 1999. It has been shown that the allochthonous microflora, which is brought into the lake with the resort effluent in mid-summer, is the anthropogenic factor contributing to an increase in the number of freshwater bacteria that feature multiple antibiotic resistance in the central part of the lake. It has been found that resistance to ampicillin of freshwater and halophile heterotrophic bacteria is related to the increase in the biomass of blue-green and green algae in the central part of Shira lake between mid-July and the end of August. A mathematical model has been constructed to describe the dynamics of the antibiotic resistance of heterotrophic bacteria in the close-to-resort and the central parts of Shira lake under natural and anthropogenic impacts.

Scopus
Держатели документа:
Centre Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Academgorodok 50, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Lobova, T.I.; Barkhatov, Yu.V.; Popova, L.Yu.

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18.

Mass exchange in an experimental new-generation life support system model based on biological regeneration of environment [Text] / A. A. Tikhomirov [et al.] ; ed. M Nelson [et al.] // SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P1711-1720, DOI 10.1016/S0273-1177(03)00108-X. - Cited References: 13 . - 10. - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Ecology + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences

Аннотация: An experimental model of a biological life support system was used to evaluate qualitative and quantitative parameters of the internal mass exchange. The photosynthesizing unit included the higher plant component (wheat and radish), and the heterotrophic unit consisted of a soil-like substrate, California worms, mushrooms and microbial microflora. The gas mass exchange involved evolution of oxygen by the photosynthesizing component and its uptake by the heterotroph component along with the formation and maintaining of the SLS structure, growth of mushrooms and California worms, human respiration, and some other processes. Human presence in the system in the form of "virtual human" that at regular intervals took part in the respirative gas exchange during the experiment. Experimental data demonstrated good oxygen/carbon dioxide balance, and the closure of the cycles of these gases was almost complete. The water cycle was nearly 100% closed. The main components in the water mass exchange were transpiration water and the watering solution with mineral elements. Human consumption of the edible plant biomass (grains and roots) was simulated by processing these products by a unique physicochemical method of oxidizing them to inorganic mineral compounds, which were then returned into the system and fully assimilated by the plants. The oxidation was achieved by "wet combustion" of organic biomass, using hydrogen peroxide following a special procedure, which does not require high temperature and pressure. Hydrogen peroxide is produced from the water inside the system. The closure of the cycle was estimated for individual elements and compounds. Stoichiometric proportions are given for the main components included in the experimental model of the system. Approaches to the mathematical modeling of the cycling processes are discussed, using the data of the experimental model. Nitrogen, as a representative of biogenic elements, shows an almost 100% closure of the cycle inside the system, The proposed experimental model of a biological system is discussed as a candidate for potential application in the investigations aimed at creating ecosystems with largely closed cycles of the internal mass exchange. The formation and maintenance of sustainable cycling of vitally important chemical elements and compounds in biological life support systems (BLSS) is an extremely pressing problem. To attain the stable functioning of biological life support systems (BLSS) and to maintain a high degree of closure of material cycles in them, it is essential to understand the character of mass exchange processes and stoichiometric proportions of the initial and synthesized components of the system. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Держатели документа:
Russian Acad Sci, Inst Biophys, Krasnoyarsk, Russia
Univ Strasbourg 1, Clermont Ferrand, France
ESA, Estec, Environm Control & Life Support Sect, Noordwijk, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Ushakova, S.A.; Manukovsky, N.S.; Lisovsky, G.M.; Kudenko, Y.A.; Kovalev, V.S.; Gubanov, V.G.; Barkhatov, Y.V.; Gribovskaya, I.V.; Zolotukhin, I.G.; Gros, J.B.; Lasseur, C...; Nelson, M \ed.\; Pechurkin, NS \ed.\; Dempster, WF \ed.\; Somova, LA \ed.\; Somo, , LA \ed.\

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19.

Population dynamics of an algal-bacterial cenosis in closed ecological system [Text] / T. I. Pisman, Y. V. Galayda, N. S. Loginova ; ed. YV Galayd // SPACE LIFE SCIENCES: CLOSED ECOLOGICAL SYSTEMS: EARTH AND SPACE APPLICATIONS. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2005. - Vol. 35: Workshop on Closed Ecological Systems (JUL, 2004, Paris, FRANCE), Is. 9. - P1579-1583, DOI 10.1016/j.asr.2005.03.073. - Cited References: 14 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Ecology + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences

Кл.слова (ненормированные):
microalgae-bacteria relationships -- photosynthates -- detritus -- Chlorella -- biotic cycle
Аннотация: The paper deals with microalgae-bacteria interrelationships in the "autotroph-heterotroph" aquatic biotic cycle. Explanations of why and how algal-bacterial ecosystems are formed still remain controversial. The paper presents results of experimental and theoretical investigations of the functioning of the algal-bacterial cenosis (the microalga Chlorella vulgaris and concomitant microflora). The Chlorella microbial community is dominated by representatives of the genus Pseudomonas. Experiments with non-sterile batch cultures of Chlorella on Tamiya medium showed that the biomass of microorganisms increases simultaneously with the increase in microalgal biomass. The microflora of Chlorella can grow on organic substances released by photosynthesizing Chlorella. Microorganisms can also use dying Chlorella cells, i.e. form a "producer-reducer" biocycle. To get a better insight into the cenosis-forming role of microalgae, a mathematical model of the "autotroph-heterotroph" aquatic biotic cycle has been constructed, taking into account the utilization of Chlorella photosynthates and dead cells by microorganisms and the contribution of the components to the nitrogen cycle. A theoretical study showed that the biomass of concomitant bacteria grown on glucose and detritus is larger than the biomass of bacteria utilizing only microalgal photosynthates, which agrees well with the experimental data. (c) 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.

Держатели документа:
Krasnoyarsk State Univ, Krasnoyarsk 660041, Russia
RAS, SB, Inst Biophys, Krasnoyarsk 660036, Russia
Krasnoyarsk State Med Acad, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pisman, T.I.; Galayda, Y.V.; Loginova, N.S.; Galayd, YV \ed.\

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20.

LUMINESCENT BACTERIAL SYMBIONTS AND COMMENSALS OF LUMINESCENT AND NONLUMINESCENT MARINE ANIMALS OF THE INDIAN-OCEAN [Text] / G. A. VYDRYAKOVA [et al.] // Microbiology. - 1995. - Vol. 64, Is. 5. - P. 589-592. - Cited References: 18 . - ISSN 0026-2617

РУБ Microbiology
Рубрики:
BIOLUMINESCENCE
SEAWATER
Аннотация: Approximately 100 fish belonging to 24 families and several representatives of cephalopods, prawns, and euphausiids were investigated for the presence of luminescent bacteria. Species identification of isolated luminescent bacteria was performed, and the frequency and ratio of their occurrence in the gastrointestinal microflora of marine animals were determined. Luminescent bacteria occurred in 23 - 65% of the fish, depending on the habitat depth, and their ratio varied from 8 to 60% of the total gastrointestinal microflora of fish. The free-living luminescent bacteria were found in 50% of the seawater samples from depths down to 1000 m. The luminescent bacterium Photobacterium phosphoreum was dominant among the isolated cultures.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
VYDRYAKOVA, G.A.; KUZNETSOV, A.M.; PRIMAKOVA, G.A.; CHUGAEVA, Y.V.; FISH, A.M.

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