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An estimate of 241Am accumulation by the macrophyte Elodea Canadensis / A. Ya. Bolsunovskij [и др.] // Doklady Akademii Nauk. - 2004. - Vol. 399, Is. 1. - С. 130-133 . - ISSN 0869-5652
Кл.слова (ненормированные):
Biomass -- Fractionation -- Minerals -- Organic compounds -- Plants (botany) -- Radioactivity -- Radioisotopes -- Accumulation -- Macrophytes -- Americium
Аннотация: The lab experiments showed that specific activity of 241Am achieves 3280В±240 Bc/g with 241Am accumulation coefficient = 16600В±2200 l/kg. The chemical fractionation results confirmed that 241Am can be strongly fixed by elodea biomass. At the end of long-term experiment the part of 241Am strongly bonded by the biomass reached 27% of total 241Am content in biomass. The accumulation process proceeds with 241Am redistribution towards decreasing of the part of exchange and adsorbed fractions and increasing of the part of organic and mineral fractions. Thus, the 241Am part in organic fractions doubled during the experiment and in mineral fraction - grew more than 10 times as compared with initial 241Am distribution.

Scopus
Держатели документа:
Inst. Biofiziki SO RAN, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovskij, A.Ya.; Zotina, T.A.; Bondareva, L.G.; Degermendzhi, A.G.

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Increased BLSS closure using mineralized human waste in plant cultivation on a neutral substrate / S. Ushakova [et al.] // Advances in Space Research. - 2009. - Vol. 44, Is. 8. - P971-978, DOI 10.1016/j.asr.2009.06.003 . - ISSN 0273-1177
Кл.слова (ненормированные):
Biological life support system -- Crops -- Human waste recycling -- Hydroponics -- Biological life support system -- Biological life support systems -- Biological productivity -- Control experiments -- Control plants -- Environmental conditions -- Expanded clay -- Human waste -- Human waste recycling -- Hydroponics -- In-plants -- Macro element -- Mineral element -- Neutral solution -- Nutrient solution -- Photosynthetic apparatus -- Pisum sativum -- Plant growth -- Scale potential -- Spring wheat -- Triticum aestivum -- Vegetation periods -- Water extracts -- Wheat straws -- Clay minerals -- Crops -- Minerals -- Mining -- Nutrients -- Pilot plants -- Plant life extension -- Potassium -- Productivity -- Recycling -- Solvent extraction -- Vegetation -- Water content -- Plant shutdowns
Аннотация: The purpose of this work was to study the full-scale potential use of human mineralized waste (feces and urine) as a source of mineral elements for plant cultivation in a biological life support system (BLSS). Plants that are potential candidates for a photosynthesizing link were grown on a neutral solution containing human mineralized waste. Spring wheat Triticum aestivum L., peas Pisum sativum L. Ambrosia cultivar and leaf lettuce Lactuca sativa L., Vitaminny variety, were used. The plants were grown hydroponically on expanded clay aggregates in a vegetation chamber in constant environmental conditions. During plant growth, a determined amount of human mineralized waste was added daily to the nutrient solution. The nutrient solution remained unchanged throughout the vegetation period. Estimated plant requirements for macro-elements were based on a total biological productivity of 0.04 kg day-1 m-2. As the plant requirements for potassium exceeded the potassium content of human waste, a water extract of wheat straw containing the required amount of potassium was added to the nutrient solution. The Knop's solution was used in the control experiments. The experimental and control plants showed no significant differences in state or productivity of their photosynthetic apparatus. A small decrease in total productivity of the experimental plants was observed, which might result in some reduction of O{cyrillic}2 production in a BLSS. В© 2009 COSPAR.

Scopus
Держатели документа:
Institute of Biophysics, SB, RAS, Akademgorodok, 660036 Krasnoyarsk, Russian Federation
Universite Blaise-Pascal, LGCB, Polytech, BP206, 63174 Aubiere cedex, France
TEC-MCT, ESA/Estec, 1 Keplerlaan, 2201 AZ Noordwijk, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Ushakova, S.; Tikhomirov, A.; Shikhov, V.; Kudenko, Yu.; Anischenko, O.; Gros, J.-B.; Lasseur, Ch.

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Production characteristics of the "higher plants-soil-like substrate" system as an element of the bioregenerative life support system / V. V. Velichko [et al.] // Advances in Space Research. - 2013. - Vol. 51, Is. 1. - P115-123, DOI 10.1016/j.asr.2012.08.003 . - ISSN 0273-1177
Кл.слова (ненормированные):
Biological-technical life support system -- Soil-like substrate -- Utilization of plant wastes -- Age groups -- Bioregenerative life support systems -- Cultivated crops -- Cyperus esculentus -- Growth chamber -- Higher plants -- Mineral element -- Nitrogen content -- Nutrient solution -- Plant wastes -- Raphanus sativus -- Root zone -- Soil-like substrate -- Study materials -- Biomass -- Carbon dioxide -- Harvesting -- Minerals -- Plants (botany) -- Soils -- Substrates -- Waste utilization -- Ecology
Аннотация: The study addresses the possibility of long-duration operation of a higher plant conveyor, using a soil-like substrate (SLS) as the root zone. Chufa (Cyperus esculentus L.), radish (Raphanus sativus L.), and lettuce (Lactuca sativa L.) were used as study material. A chufa community consisting of 4 age groups and radish and lettuce communities consisting of 2 age groups were irrigated with a nutrient solution, which contained mineral elements extracted from the SLS. After each harvest, inedible biomass of the harvested plants and inedible biomasses of wheat and saltwort were added to the SLS. The amounts of the inedible biomasses of wheat and saltwort to be added to the SLS were determined based on the nitrogen content of the edible mass of harvested plants. CO2 concentration in the growth chamber was maintained within the range of 1100-1700 ppm. The results of the study show that higher plants can be grown quite successfully using the proposed process of plant waste utilization in the SLS. The addition of chufa inedible biomass to the SLS resulted in species-specific inhibition of growth of both cultivated crops and microorganisms in the "higher plants - SLS" system. There were certain differences between the amounts of some mineral elements removed from the SLS with the harvested edible biomass and those added to it with the inedible biomasses of wheat and saltwort. В© 2012 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of Russian Academy of Science, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Velichko, V.V.; Tikhomirov, A.A.; Ushakova, S.A.; Tikhomirova, N.A.; Shihov, V.N.; Tirranen, L.S.; Gribovskaya, I.A.

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A biological method of including mineralized human liquid and solid wastes into the mass exchange of bio-technical life support systems / S. A. Ushakova [et al.] // Advances in Space Research. - 2012. - Vol. 50, Is. 7. - P932-940, DOI 10.1016/j.asr.2012.05.023 . - ISSN 0273-1177
Кл.слова (ненормированные):
Bio-technical life support system -- Conveyor-grown uneven-aged communities of wheat and Salicornia -- Utilization of human wastes -- Age groups -- Biological methods -- Dry weight -- Expanded clay -- Harvest index -- Human waste -- Leafy vegetables -- Liquid wastes -- Mass exchange -- Mineral element -- Nutrient solution -- Plant communities -- Salicornia europaea -- Salt content -- Time interval -- Uneven-aged -- Water culture -- Wheat biomass -- Conveyors -- Incineration -- Irrigation -- Minerals -- Nutrients -- Plants (botany) -- Productivity -- Sodium chloride -- Waste incineration -- Waste utilization -- Liquids
Аннотация: The main obstacle to using mineralized human solid and liquid wastes as a source of mineral elements for plants cultivated in bio-technical life support systems (BLSS) is that they contain NaCl. The purpose of this study is to determine whether mineralized human wastes can be used to prepare the nutrient solution for long-duration conveyor cultivation of uneven-aged wheat and Salicornia europaea L. plant community. Human solid and liquid wastes were mineralized by the method of "wet incineration" developed by Yu. Kudenko. They served as a basis for preparing the solutions that were used for conveyor-type cultivation of wheat community represented by 5 age groups, planted with a time interval of 14 days. Wheat was cultivated hydroponically on expanded clay particles. To reduce salt content of the nutrient solution, every two weeks, after wheat was harvested, 12 L of solution was removed from the wheat irrigation tank and used for Salicornia europaea cultivation in water culture in a conveyor mode. The Salicornia community was represented by 2 age groups, planted with a time interval of 14 days. As some portion of the nutrient solution used for wheat cultivation was regularly removed, sodium concentration in the wheat irrigation solution did not exceed 400 mg/L, and mineral elements contained in the removed portion were used for Salicornia cultivation. The experiment lasted 4 months. The total wheat biomass productivity averaged 30.1 gВ·m -2В·day -1, and the harvest index amounted to 36.8%. The average productivity of Salicornia edible biomass on a dry weight basis was 39.3 gВ·m -2В·day -1, and its aboveground mass contained at least 20% of NaCl. Thus, the proposed technology of cultivation of wheat and halophyte plant community enables using mineralized human wastes as a basis for preparing nutrient solutions and including NaCl in the mass exchange of the BLSS; moreover, humans are supplied with additional amounts of leafy vegetables. В© 2012 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Siberian State Technological University, 82, Mir Avenue, Krasnoyarsk 660049, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Ushakova, S.A.; Tikhomirov, A.A.; Tikhomirova, N.A.; Kudenko, Y.A.; Litovka, Y.A.; Anishchenko, O.V.

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Some methods for human liquid and solid waste utilization in bioregenerative life-support systems / S. A. Ushakova [et al.] // Applied Biochemistry and Biotechnology. - 2008. - Vol. 151, Is. 2-3. - P676-685, DOI 10.1007/s12010-008-8291-3 . - ISSN 0273-2289
Кл.слова (ненормированные):
Closure -- Human waste -- Life-support systems -- Salicornia -- Sodium chloride -- Above-ground biomass -- Biological lives -- Bioregenerative -- Closure -- Cultivation process -- Culture methods -- Human waste -- Irrigation waters -- Life-support systems -- Manned space missions -- Mineral elements -- Physico-chemical methods -- Salicornia -- Salicornia europaea -- Salt-tolerant -- Soil-like substrates -- Biomass -- Body fluids -- Electrodialysis -- Grain (agricultural product) -- Irrigation -- Liquids -- Metal refining -- Minerals -- Mining -- Oxidation -- Plant shutdowns -- Sodium chloride -- Soils -- Solid wastes -- Substrates -- Water supply -- Vegetation -- article -- biomass -- controlled study -- electrodialysis -- halophyte -- irrigation (agriculture) -- microclimate -- nonhuman -- recycling -- Salicornia europaea -- solid waste -- bioremediation -- dialysis -- feces -- goosefoot -- growth, development and aging -- human -- methodology -- salt tolerance -- urine -- waste management -- wheat -- Batis maritima -- Salicornia -- Salicornia europaea -- Triticum aestivum -- Biodegradation, Environmental -- Chenopodiaceae -- Dialysis -- Feces -- Humans -- Life Support Systems -- Salt-Tolerance -- Triticum -- Urine -- Waste Management
Аннотация: Bioregenerative life-support systems (BLSS) are studied for developing the technology for a future biological life-support system for long-term manned space missions. Ways to utilize human liquid and solid wastes to increase the closure degree of BLSS were investigated. First, urine and faeces underwent oxidation by Kudenko's physicochemical method. The products were then used for root nutrition of wheat grown by the soil-like substrate culture method. Two means of eliminating sodium chloride, introduced into the irrigation solution together with the products of urine oxidation, were investigated. The first was based on routine electrodialysis of irrigation water at the end of wheat vegetation. Dialysis eliminated about 50% of Na from the solution. This desalinization was performed for nine vegetations. The second method was new: after wheat cultivation, the irrigation solution and the solution obtained by washing the substrate containing mineral elements not absorbed by the plants were used to grow salt-tolerant Salicornia europaea L. plants (saltwort). The above-ground biomass of this plant can be used as a food, and roots can be added to the soil-like substrate. Four consecutive wheat and Salicornia vegetations were cultivated. As a result of this wheat and Salicornia cultivation process, the soil-like substrate salinization by NaCl were considerably decreased. В© 2008 Humana Press.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Science, Siberian Branch, 660036 Krasnoyarsk, Russian Federation
K.A. Timiraziev Institute of Plant Physiology, Russian Academy of Science, 35 Botanisheskaya, 127276 Moscow, Russian Federation
LGCB, Universite Blaise Pascal, Polytech'Clermont-Ferrand, P.O. Box 206, 63174 Aubiere cedex, France : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Ushakova, S.A.; Zolotukhin, I.G.; Tikhomirov, A.A.; Tikhomirova, N.A.; Kudenko, Yu.A.; Gribovskaya, I.V.; Balnokin, Yu.; Gros, J.B.

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Possibility of Salicornia europaea use for the human liquid wastes inclusion into BLSS intrasystem mass exchange / N. A. Tikhomirova [et al.] // Acta Astronautica. - 2008. - Vol. 63, Is. 7-10. - P1106-1110, DOI 10.1016/j.actaastro.2008.01.003 . - ISSN 0094-5765
Кл.слова (ненормированные):
BLSS -- NaCl turnover -- Salicornia europaea -- Space biology -- Acids -- Amides -- Arsenic compounds -- Biochemical engineering -- Biochemistry -- Biomass -- Cellulose -- Curing -- Drying -- Environmental engineering -- Fatty acids -- Garnets -- Health -- Human engineering -- Lipids -- Mineralogy -- Minerals -- Mining -- Nitrates -- Nitrogen -- Plants (botany) -- Polysaccharides -- Polyvinyl alcohols -- Renewable energy resources -- Silica -- Silicate minerals -- Sodium -- Sodium chloride -- Solutions -- Sugar (sucrose) -- Sugars -- Waste utilization -- Biochemical composition -- Biochemical substances -- Bioregenerative life support systems (BLSS) -- Component conditions -- Crude protein (CP) -- Dry weight (DW) -- Essential fatty acids -- Europaea -- H igh concentrations -- Human urine -- Linoleic acid (LA) -- Linolenic -- Lipid content -- Liquid wastes -- Mass exchanges -- Mineral compositions -- Nitrate nitrogen -- Nitrogen nutrition -- Non saturation -- Physico chemical processes -- Plant functions -- Plant lipids -- Reduced nitrogen -- Salicornia europaea -- Soluble sugars -- Wide spectrum -- Nonmetals -- Amides -- Arsenic -- Biochemistry -- Biomass -- Biotechnology -- Cellulose -- Curing -- Drying -- Fatty Acids -- Lipids -- Nonmetals -- Plants -- Polysaccharides -- Sugars
Аннотация: One of the ways of solving the problem of the human liquid wastes utilization in bioregenerative life support systems (BLSS) can be the use of halophytic vegetable plant Salicornia europaea capable of accumulating sodium chloride in rather high concentrations. Since the most specific higher plant function in BLSS, which at present cannot be substituted by physicochemical processes, appears to be the biosynthesis of a wide spectrum of nutritive substances necessary for a human, the object of the given work was the investigation of the S. europaea productivity, biochemical and mineral composition when grown under close to optimal BLSS vegetative component conditions. As the use of human urine after its preliminary physicochemical processing is supposed to be the mineral solution basis for the S. europaea cultivation, it is necessary to clear up the effect of reduced nitrogen on plants growth. Ground research was carried out. Biochemical composition of the S. europaea edible part showed that crude protein was contained in the highest degree. At that the content of crude protein (24% per dry weight) and cellulose (4.7% per dry weight) was higher in the plants grown on solutions containing amide nitrogen in comparison with the plants grown on solutions with nitrate nitrogen (15.4%-3.1% correspondingly). The water-soluble sugar contents were not high in the S. europaea edible part and depending on the nitrogen nutrition form they amounted to 1.1% (amide nitrogen) and 1.5% (nitrate nitrogen). The polysaccharide number (except cellulose) was rather higher and varied from 7.7% to 8.2%. Although the lipid content in the S. europaea plants was relatively low (7% per dry weight), it was shown that the plant lipids are characterized by a high nonsaturation degree mainly due to alpha linolenic and linoleic acids. Nitrogen nutrition form did not significantly affect the S. europaea productivity, and dry edible biomass of one plant was 8.6 g. Sodium and its concentrations predominated in the plant mineral composition and amounted in average to 9% per dry weight. Thus the S. europaea being the vegetable plant it can be the source of several biochemical substances and essential fatty acids. The present work also considers the influence of nitrate and amide forms of nitrogen on S. europaea biochemical and mineral composition. В© 2008 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Akademgorodok, 660036 Krasnoyarsk, Russian Federation
LGCB, Universite B. Pascal, CUST, BP206, 63174 Aubie're, cedex, France : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirova, N.A.; Ushakova, S.A.; Tikhomirov, A.A.; Kalacheva, G.S.; Gros, J.-B.

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Potential of salt-accumulating and salt-secreting halophytic plants for recycling sodium chloride in human urine in bioregenerative life support systems / N. A. Tikhomirova [et al.] // Advances in Space Research. - 2011. - Vol. 48, Is. 2. - P378-382, DOI 10.1016/j.asr.2011.03.016 . - ISSN 0273-1177
Кл.слова (ненормированные):
BLSS -- Limonium gmelinii -- Mineral nutrition -- NaCl -- Salicornia europaea -- BLSS -- Limonium gmelinii -- Mineral nutrition -- NaCl -- Salicornia europaea -- Body fluids -- Pilot plants -- Recycling -- Silicate minerals -- Sodium chloride -- Plants (botany)
Аннотация: This study addresses the possibility of growing different halophytic plants on mineralized human urine as a way to recycle NaCl from human wastes in a bioregenerative life support system (BLSS). Two halophytic plant species were studied: the salt-accumulating Salicornia europaea and the salt-secreting Limonium gmelinii. During the first two weeks, plants were grown on Knop's solution, then an average daily amount of urine produced by one human, which had been preliminarily mineralized, was gradually added to the experimental solutions. Nutrient solutions simulating urine mineral composition were gradually added to control solutions. NaCl concentrations in the stock solutions added to the experimental and control solutions were 9 g/L in the first treatment and 20 g/L in the second treatment. The mineralized human urine showed some inhibitory effects on S. europaea and L. gmelinii. The biomass yield of experimental plants was lower than that of control ones. If calculated for the same time period (120 d) and area (1 m 2), the amount of sodium chloride taken up by S. europaea plants would be 11.7 times larger than the amount taken up by L. gmelinii plants (486 g/m 2 vs. 41 g/m 2). Thus, S. europaea is the better choice of halophyte for recycling sodium chloride from human wastes in BLSS. В© 2011 COSPAR. Published by Elsevier Ltd. All rights reserved. 25.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, 50 Akademgorodok, Krasnoyarsk 660036, Russian Federation
K.A. Timiryazev Plant Physiology Institute, Russian Academy of Sciences, 35 Botanicheskaya St., Moscow 127276, Russian Federation
Universite Blaise Pascal, LGCB, Polytech, BP 206, 36174 Aubiere, France
TFC-MCT, ESA/Estec, 1 Keplerlaan, 2201 AG Noordwijk, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirova, N.A.; Ushakova, S.A.; Kudenko, Yu.A.; Gribovskaya, I.V.; Shklavtsova, E.S.; Balnokin, Yu.V.; Popova, L.G.; Myasoedov, N.A.; Gros, J.-B.; Lasseur, Ch.

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Effect of NaCl concentration on productivity and mineral composition of Salicornia europaea as a potential crop for utilization NaCl in LSS / S. A. Ushakova [et al.] // Advances in Space Research. - 2005. - Vol. 36, Is. 7. - P1349-1353, DOI 10.1016/j.asr.2004.09.017 . - ISSN 0273-1177
Кл.слова (ненормированные):
Life support system -- NaCl -- Salicornia europaea -- Space biology -- Calcium -- Concentration (process) -- Minerals -- Photosynthesis -- Plants (botany) -- Salinity measurement -- Sodium chloride -- Vegetation -- Life support systems -- Liquid wastes -- NaCl -- Salicornea europea -- Space biology -- Space research
Аннотация: The accumulation of solid and liquid wastes in manmade ecosystems presents a problem that has not been efficiently solved yet. Urine, containing NaCl, are part of these products. This is an obstacle to the creation of biological systems with a largely closed material cycling, because the amount of solid and liquid wastes in them must be reduced to a minimum. A possible solution to the problem is to select plant species capable of utilizing sufficiently high concentrations of NaCl, edible for humans, and featuring high productivity. Until recently, the life support systems have included the higher plants that were either sensitive to salinization (wheat, many of the legumes, carrot, potato, maize) or relatively salt-resistant (barley, sugar beet, spinach). Salicomia europaea, whose above-ground part is fully edible for humans, is one of the most promising candidates to be included in life support systems. It is reported in the literature that this plant is capable of accumulating up to 50% NaCl (dry basis). Besides, excessive accumulation of sodium ions should bring forth a decrease in the uptake of potassium ions and other biogenic elements. The aim of this work is to study the feasibility of using S. europaea plants in growth chambers to involve NaCl into material cycling. Plants were grown in vegetation chambers at the irradiance of 100 or 150 W/m2 PAR (photosynthetically active radiation) and the air temperature 24 В°C, by two methods. The first method was to grow the plants on substrate - peat. The peat was supplemented with either 3% NaCl (Variant 1) or 6% NaCl (Variant 2) of the oven-dry mass of the peat. The second method was to grow the plants in water culture, using the solution with a full complement of nutrients, which contained 0.0005% of NaCl, 1% or 2%. The study showed that the addition of NaCl to the substrate or to the solution resulted in the formation of more succulent plants, which considerably increased their biomass. The amount of NaCl uptake was the highest in the plants grown in water culture, 2.6 g per plant. As the sodium uptake increased, the consumption of potassium and the sum of the reduced N forms decreased twofold. The uptake of calcium and magnesium by plants decreased as the NaCl concentration increased; the smallest amounts were taken up by S. europaea grown in water culture. Salinity had practically no effect on the uptake of phosphorus and sulfur. Thus, S. europaea is a promising candidate to be included in life support systems; of special interest is further research on growing these plants in water culture. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Science, Siberian Branch, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Ushakova, S.A.; Kovaleva, N.P.; Gribovskaya, I.V.; Dolgushev, V.A.; Tikhomirova, N.A.

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Survival and alteration of the plasmid-containing microorganism Escherichia coli Z905/pPHL7 introduced into manmade closed aquatic microcosms / A. N. Boyandin [et al.] // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1763-1768, DOI 10.1016/S0273-1177(03)00118-2 . - ISSN 0273-1177
Кл.слова (ненормированные):
Cells -- Escherichia coli -- Salts -- Recombinant plamid -- Space research -- ampicillin -- chloride -- inorganic salt -- magnesium -- mineral -- potassium -- sodium -- sulfate -- genetically modified organism -- article -- bacterial count -- chemoluminescence -- culture medium -- drug effect -- Escherichia coli -- genetics -- growth, development and aging -- microbiology -- microclimate -- penicillin resistance -- plasmid -- Russian Federation -- transgenic organism -- Ampicillin -- Ampicillin Resistance -- Chemiluminescent Measurements -- Chlorides -- Colony Count, Microbial -- Culture Media -- Ecological Systems, Closed -- Escherichia coli -- Magnesium -- Minerals -- Organisms, Genetically Modified -- Plasmids -- Potassium -- Russia -- Salts -- Sodium -- Sulfates -- Water Microbiology
Аннотация: It has been demonstrated that the transgenic microorganism Escherichia coli Z905/pPHL7 (Ap'Lux+) can exist for a long time at an elevated concentration of mineral salts. The microorganism was introduced into microcosms with sterile brackish water (salinity variable from 21 to 22 g 1-1) taken from Lake Shira (Khakasia, Russia). The survivof the microorganism was estimated both by measuring the growth of the colonies on solid nutrient media and by the bioluminescence exhibited by the transgenic strain in samples from the microcosms and in the enrichment culture with the added selective factor - ampicillin (50 ?g/ml). In the enrichment culture, the bioluminescent signal was registered through the 160-day experiment. It has been shown that in the closed microcosms with brackish water the E. coli strain becomes heterogeneous in its ampicillin resistance. The populations of the transgenic strain were mainly represented by isolates able to persist in the medium containing 50 ?g/ml, but there were also the cells (about 10%) with the threshold of ampicillin resistance not more than 0.05 ?g/ml. Thus, it was shown that in the microcosms with brackish water and in the absence of the selective factor the transgenic strain survives and retains the recombinant plasmid. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

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Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Lobova, T.I.; Popova, L.Yu.; Pechurkin, N.S.

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10.

In vivo study of 2D PHA matrices of different chemical compositions: Tissue reactions and biodegradations [] / T. G. Volova [et al.] // Mater. Sci. Technol. - 2014. - Vol. 30, Is. 5. - P549-557, DOI 10.1179/1743284713Y.0000000470 . - ISSN 1743-2847
Кл.слова (ненормированные):
Biocompatibility -- Biodegradation -- Implantation -- PHA -- Polyhydroxyalkanoates -- Tissue response -- Biocompatibility -- Biodegradable polymers -- Copolymers -- Degradation -- Ion implantation -- Microbiology -- Tissue -- Chemical compositions -- Foreign body giant cells -- Hydroxybutyric acids -- Maximum thickness -- PHA -- Polyhydroxyalkanoates -- Polymer matrices -- Tissue response -- Biodegradation
Аннотация: Matrices based on resorbable polyhydroxyalkanoates (PHAs) of five types {a homopolymer of 3- hydroxybutyric acid, copolymers of 3-hydroxybutyric and 4-hydroxybutyric acids [P(3HB/4HB)], 3-hydroxybutyric and 3-hydroxyvaleric acids [P(3HB/3HV)], 3-hydroxybutyric and 3-hydroxyhexanoic acids [P(3HB/3HHx)]} have been constructed and characterised. No significant differences have been found in tissue response to implantation of these PHAs. Non-coarse fibrous capsules that formed around PHA matrices reached their maximum thickness (60-90 ?m) 90 days after implantation; by day 180, the average thickness of the capsules had decreased by 1·5-2·3 times. The number of foreign body giant cells, resorbing PHAs, remained high. In vivo biodegradation behaviour of polymer matrices is related to the chemical composition of the PHA. Matrices prepared from copolymers P(3HB/4HB) and P(3HB/3HHx) exhibited the fastest degradation rates. P3HB/3HV matrices were degraded more slowly, and P3HB matrices were the most durable. In the PHA matrices that were degraded more slowly, giant cell reaction developed later. © 2014 Institute of Materials, Minerals and Mining.

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Держатели документа:
Institute of Biophysics SB RAS, Akademgorodok 50, Krasnoyarsk 660036, Russian Federation
Institute of Modern Biology and Biotechnology, Siberian Federal University, Svobodniy av. 79, Krasnoyarsk 660041, Russian Federation
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, United States
Engineering Systems Division, Massachusetts Institute of Technology, Cambridge, MA 02139, United States
Health Sciences Technology Division, Massachusetts Institute of Technology, Cambridge, MA 02139, United States : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Shishatskaya, E.I.; Nikolaeva, E.D.; Sinskey, A.J.

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11.

Effects of mineral nutrition conditions on heat tolerance of chufa (Cyperus esculentus L.) plant communities to super optimal air temperatures in the BTLSS / E. S. Shklavtsova [et al.] // Adv. Space Res. - 2014. - Vol. 54, Is. 6. - P1135-1145, DOI 10.1016/j.asr.2014.05.031 . - ISSN 1879-1948
Кл.слова (ненормированные):
Bioregenerative life support system -- Chlorophyll fluorescence -- Cyperus esculentus L. -- Heat shock -- Lipid peroxidation -- Mineralized human wastes -- Atmospheric temperature -- Carbon dioxide -- Lipids -- Metabolism -- Minerals -- Nitrates -- Nitrogen -- Nutrition -- Plants (botany) -- Urea -- Wastes -- Bioregenerative life support systems -- Chlorophyll fluorescence -- Cyperus esculentus -- Heat-shock -- Human waste -- Lipid peroxidation -- Plant shutdowns
Аннотация: The use of mineralized human wastes as a basis for nutrient solutions will increase the degree of material closure of bio-technical human life support systems. As stress tolerance of plants is determined, among other factors, by the conditions under which they have been grown before exposure to a stressor, the purpose of the study is to investigate the level of tolerance of chufa (Cyperus esculentus L.) plant communities grown in solutions based on mineralized human wastes to a damaging air temperature, 45 °C. Experiments were performed with 30-day-old chufa plant communities grown hydroponically, on expanded clay aggregate, under artificial light, at 690 ?mol m-2 s-1 PAR and at a temperature of 25 °C. Plants were grown in Knop's solution and solutions based on human wastes mineralized according to Yu.A. Kudenko's method, which contained nitrogen either as ammonium and urea or as nitrates. The heat shock treatment lasted 20 h at 690 and 1150 ?mol m -2 s-1 PAR. Chufa heat tolerance was evaluated based on parameters of CO2 gas exchange, the state of its photosynthetic apparatus (PSA), and intensity of peroxidation of leaf lipids. Chufa plants grown in the solutions based on mineralized human wastes that contained ammonium and urea had lower heat tolerance than plants grown in standard mineral solutions. Heat tolerance of the plants grown in the solutions based on mineralized human wastes that mainly contained nitrate nitrogen was insignificantly different from the heat tolerance of the plants grown in standard mineral solutions. A PAR intensity increase from 690 ?mol m -2 s-1 to 1150 ?mol m-2 s-1 enhanced heat tolerance of chufa plant communities, irrespective of the conditions of mineral nutrition under which they had been grown. © 2014 COSPAR. Published by Elsevier Ltd. All rights reserved.

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Держатели документа:
SB RAS Institute of Biophysics, 660036 Akademgorodok, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shklavtsova, E.S.; Ushakova, S.A.; Shikhov, V.N.; Anishchenko, O.V.

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12.

Processing of sanitary and household waste in biotechnical life support systems / S. V. Trifonov, Ye. A. Morozov, A. A. Tikhomirov // IOP Conference Series: Materials Science and Engineering : Institute of Physics Publishing, 2019. - Vol. 537: International Workshop on Advanced Technologies in Material Science, Mechanical and Automation Engineering - MIP: Engineering-2019 (4 April 2019 through 6 April 2019, ) Conference code: 149243, Is. 6, DOI 10.1088/1757-899X/537/6/062091
Кл.слова (ненормированные):
Electric fields -- Fertilizers -- Wastes -- Alternating current -- Cotton wastes -- Household waste -- Kitchen wastewaters -- Mineral element -- Mineral fertilizers -- Organic wastes -- Universal method -- Minerals
Аннотация: In order to establish material loops in biotechnical life support systems (BTLSS), various types of organic waste generated within the system need to be effectively processed. A universal method should be developed to produce mineral fertilizers for the higher-plant compartment of the BTLSS regardless of the level of reduction of the organic compounds contained in the waste and the contents of mineral elements in it. A method of producing mineral fertilizers by organic waste oxidation in the hydrogen peroxide aqueous solution under application of an alternating current electric field is proposed as a possible approach. Methods of processing of human wastes and inedible plant biomass were discussed in previous studies. The present study demonstrates the approach to processing cotton waste and kitchen wastewater. The study describes processing of such wastes by using a supplementary oxidizer (nitric acid) and co-oxidation with other types of organic waste typically generated in the BTLSS. Recommendations are offered on using these approaches to process sanitary/household waste in the BTLSS. © 2019 IOP Publishing Ltd. All rights reserved.

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Держатели документа:
Institute of Biophysics, SB RAS, Federal Research Center, Krasnoyarsk Science Center SB RAS, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Reshetnev Siberian State University of Science and Technology, 31 Krasnoyarskiy Rabochiy pr., Krasnoyarsk, 660037, Russian Federation

Доп.точки доступа:
Trifonov, S. V.; Morozov, Ye. A.; Tikhomirov, A. A.

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