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Surface modification of bioresorbable polymer scaffolds by laser treatment / V. V. Slabko [et al.] // Biophysics. - 2010. - Vol. 55, Is. 2. - P234-238, DOI 10.1134/S0006350910020120 . - ISSN 0006-3509
Кл.слова (ненормированные):
hydrophilicity -- laser irradiation -- microbial biocompatible and biodegradable polymers -- polyhydroxybuturate -- surface properties
Аннотация: The effect of laser irradiation on the properties of the surface of films prepared from a bioresorbable polymer poly(hydroxybuturate) has been studied. To determine the spectral region of the polymer optimal for the effective action of radiation on electron molecular bonds, theoretical investigations have been performed, which have shown that, for modifying the surface of PHB scaffolds, it is expedient to use a vacuum laser at a wavelength of 160 nm. Using laser irradiation at a power from 3 to 30 W, a series of films with modified surface, from roughnesses to perforations, have been obtained. The microstructure and properties of the film surface depending on the mode of irradiation have been examined, and conditions have been found under which the contact marginal angles of film wetting with water can be decreased to 50В° (compared with 76-80В° in starting products). Thus, conditions of laser treatment of PHB scaffolds have been theoretically substantiated and experimentally realized that provide a beneficial effect on the properties of the surface without destroying the structure of the material. В© 2010 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Biophysics, Russian Academy of Sciences, Siberian Division, Krasnoyarsk 660036, Russian Federation
Siberian State Technological University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Slabko, V.V.; Volova, T.G.; Krasnov, P.O.; Kuzubov, A.A.; Shishatskaya, E.I.

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Physiological and biochemical properties of the alga Botryococcus braunii / T. G. Volova [et al.] // Russian Journal of Plant Physiology. - 1998. - Vol. 45, Is. 6. - P775-779 . - ISSN 1021-4437
Кл.слова (ненормированные):
Batch culture -- Botryococcus braunii -- Liquid hydrocarbons -- Productivity
Аннотация: The growth, productivity, and changes in the chemical composition of the green alga Botryococcus braunii Kutz H-252 were studied at different phases of growth in a luminostat culture. Modification of the Prat medium resulted in an increase in the alga yield to 3.9 g/l and a decrease in the generation time to 3-4 days. The specific growth rate during the exponential phase was 0.235/ day. These values are comparable with the best results obtained abroad. Among the pigments of B. braunii, besides chlorophylls a and b, carotene, lutein, neoxanthin, and violaxanthin were also identified. The highest content of these pigments, based on dry weight, was achieved during the exponential growth phase. During culture aging, there was an accumulation of carbohydrates and lipids in addition to a simultaneous decrease in the concentration of nitrogen-containing substances. It was discovered that this strain can synthesize liquid hydrocarbons. These hydrocarbons were found both in the alga biomass (ca. 1% of dry weight) and among the extracellular metabolites (up to 10.3 mg/l).

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Zhilo, N.O.; Plotnikov, V.F.

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Characterization of field emission cathodes with different forms of diamond coatings [Text] / V. V. Zhirnov [et al.] // J. Vac. Sci. Technol. B. - 1999. - Vol. 17: 11th International Vacuum Microelectronics Conference (IVMC 98) (JUL 19-23, 1998, ASHEVILLE, NORTH CAROLINA), Is. 2. - P666-669, DOI 10.1116/1.590614. - Cited References: 9 . - 4. - ISSN 1071-1023

РУБ Engineering, Electrical & Electronic + Nanoscience & Nanotechnology + Physics, Applied
Рубрики:
TIPS
   SILICON
   GROWTH
   ARRAYS
   FILMS
Аннотация: The emission of different, diamond coatings on silicon field emitter. arrays were investigated: chemical vapor deposition diamond, high pressure and high temperature synthetic diamond, and shock-synthesized nanodiamond. Practical emission characteristics such as: emission threshold, maximum current, current stability, and reproducibility were tested. The effects of surface modification, the size of diamond crystallites, and hydrogen plasma treatments were also studied. (C) 1999 American Vacuum Society. [S0734-211X(99)06102-8].

Держатели документа:
Inst Crystallog, Moscow 117333, Russia
Univ Fribourg, Dept Phys, CH-1700 Fribourg, Switzerland
Russian Fed Nucl Ctr, Snezhinsk 456770, Russia
Inst Biophys, Krasnoyarsk 660036, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zhirnov, V.V.; Kuttel, O.M.; Groning, O...; Alimova, A.N.; Detkov, P.Y.; Belobrov, P.I.; Maillard-Schaller, E...; Schlapbach, L...

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Electrophoresis of nanodiamond powder for cold cathode fabrication [Text] / A. N. Alimova [et al.] // J. Vac. Sci. Technol. B. - 1999. - Vol. 17: 11th International Vacuum Microelectronics Conference (IVMC 98) (JUL 19-23, 1998, ASHEVILLE, NORTH CAROLINA), Is. 2. - P715-718, DOI 10.1116/1.590625. - Cited References: 5 . - 4. - ISSN 1071-1023

РУБ Engineering, Electrical & Electronic + Nanoscience & Nanotechnology + Physics, Applied
Рубрики:
FIELD-EMISSION
TIPS
Аннотация: The process of electrophoresis/dielectrophoresis of shock-synthesized nanodiamond powders on the large-area Si field emission arrays was studied. The process was found to be very sensitive to the modification of nanodiamond powder. The growth rate and film morphology of nanodiamond coatings on Si tips were investigated. Results in fabrication and testing of nanodiamond-based cold cathodes and sealed device prototypes are presented. (C) 1999 American Vacuum Society. [S0734-211X(99)06002-3].

Держатели документа:
Russian Acad Sci, Inst Crystallog, Moscow 117333, Russia
ISTOK, State Res & Prod Corp, Fryazino 141120, Moscow Region, Russia
Russian Acad Sci, Inst Biophys, Krasnoyarsk 660036, Russia
Russian Fed Nucl Ctr, Snezhinsk 456770, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Alimova, A.N.; Chubun, N.N.; Belobrov, P.I.; Detkov, P.Y.; Zhirnov, V.V.

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Recombinant Metridia luciferase isoforms: expression, refolding and applicability for in vitro assay [Text] / V. V. Borisova [et al.] // Photochem. Photobiol. Sci. - 2008. - Vol. 7, Is. 9. - P1025-1031, DOI 10.1039/b807271j. - Cited References: 19. - The work was supported by Bayer AG, by the Russian Foundation for Basic Research grants 05-04-48271 and 06-04-08076, by the joint grant 06-04-89502 of the Russian Foundation for Basic Research and Taiwan National Science Council, and by the "Molecular and Cellular Biology" program of the Russian Academy of Sciences. . - ISSN 1474-905X

РУБ Biochemistry & Molecular Biology + Biophysics + Chemistry, Physical
Рубрики:
BIOLUMINESCENT REPORTER
   GAUSSIA LUCIFERASE
   CDNA
   PROTEINS
   CLONING
   OVEREXPRESSION
   PURIFICATION
   MUTAGENESIS
   ENZYME
   OBELIN
Аннотация: The recombinant coelenterazine-dependent luciferases (isoforms MLuc 164 and MLuc39) from the marine copepod Metridia longa were expressed as inclusion bodies in E. coli cells, dissolved in 6 M guanidinium chloride and folded in conditions developed for proteins containing intramolecular disulfide bonds. One of them (MLuc09) was obtained in an active monomeric form with a high yield. The luciferase bioluminescence is found to be initiated not only by free coelenterazine, but also by Ca2+-dependent coelenterazine-binding protein (CBP) of Renilla muelleri on Ca2+ addition. The use of CBP as a "substrate" provides higher light emission and simultaneously the lower level of background. The high purity MLuc39 can be detected down to attomol with a linear range extending over 5 orders of magnitude. The MLuc39 reveals also a high stability towards heating and chemical modification; the chemically synthesized biotinylated derivatives of the luciferase preserve 35-40% of the initial activity The luciferase applicability as an in vitro bioluminescent reporter is demonstrated in model tandem bioluminescent solid-phase microassay combining the Ca2+-regulated photoprotein obelin and the Metridia luciferase.

Держатели документа:
[Borisova, Vasillisa V.
Frank, Ludmila A.
Markova, Svetlana V.
Burakova, Ludmilla P.
Vysotski, Eugene S.] Russian Acad Sci, Inst Biophys, Photobiol Lab, Siberian Branch, Krasnoyarsk 660036, Russia
[Frank, Ludmila A.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Borisova, V.V.; Frank, L.A.; Markova, S.V.; Burakova, L.P.; Vysotski, E.S.

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Physical and chemical properties of modified nanodiamonds [Text] / A. P. Puzyr [et al.] ; ed.: DM Gruen, OA Shenderova, erova, OA She // Synthesis, Properties and Applications of Ultrananocrystalline Diamond. Ser. NATO SCIENCE SERIES, SERIES II: MATHEMATICS, PHYSICS AND CHEMISTRY : SPRINGER, 2005. - Vol. 192: NATO Advanced Research Workshop on Synthesis, Properties and Applications of Ultrananocrystalline Diamond (JUN 07-10, 2004, St Petersburg, RUSSIA). - P261-270. - Cited References: 15 . - ISBN 1-4020-3320-6

РУБ Chemistry, Physical + Materials Science, Multidisciplinary

Кл.слова (ненормированные):
nanodiamond -- modified nanodiamonds -- hydrosols -- colloidal stability -- electrophoresis -- powder fractioning -- precursors for CVD -- electron field emission
Аннотация: A unique technology of nanodiamond surface modification is suggested which allows to separation of commercial nanodiamond powders into two fractions (F1 and 172), each possessing absolutely new properties as compared to the initial powder. F1 and F2 differ in size characteristics. Initial and modified nanodiamonds contain iron impurities and two types of nondiamond carbon. The color of the powders and hydrosols does not correlate with the content of non-diamond carbon. According to the EPR data, modified nanodiamonds possess a high level of diamond matrix shielding, and the extracted fractions differ in width of the basic transition area and in the SHF energy adsorption ratio. Due to this, F1 can be applied as precursors for CVD growth of nanocrystalline diamond and as field electron emission tips.

Держатели документа:
RAS, Inst Biophys SB, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Puzyr, A.P.; Bondar, V.S.; Bukayemsky, A.A.; Selyutin, G.E.; Kargin, V.F.; Gruen, DM \ed.\; Shenderova, OA \ed.\; She, erova, OA \ed.\

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AsLn2, a luciferin-related modified tripeptide from the bioluminescent earthworm Fridericia heliota / V. N. Petushkov [et al.] // Tetrahedron Letters. - 2014. - Vol. 55, Is. 2. - P463-465, DOI 10.1016/j.tetlet.2013.11.061 . - ISSN 0040-4039
Кл.слова (ненормированные):
Bioluminescence -- Fridericia heliota -- Luciferin -- Modified peptide
Аннотация: AsLn2, an unusual modified peptide, was isolated from the bioluminescent earthworm Fridericia heliota (Enchytraeidae). Its structure, elucidated by NMR and mass spectrometry, includes residues of tyrosine, CompX (a novel tyrosine modification product, reported in the accompanying paper), and N(omega)-acylated lysine. Chromatography, UV, and 1H NMR data imply a close structural similarity of AsLn2 with F. heliota luciferin. AsLn2 appears to be an intermediate or by-product in F. heliota luciferin biosynthesis. © 2013 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Laboratory of Bioluminescent Biotechnologies, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, pr. Svobodnyi, 79, Krasnoyarsk 660041, Russian Federation
Laboratory of Photobiology, Institute of Biophysics, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russian Federation
Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, Campo Alegre St. 687, Porto 4169-007, Portugal
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V.N.; Dubinnyi, M.A.; Rodionova, N.S.; Nadezhdin, K.D.; Marques, S.M.; Esteves Da Silva, J.C.G.; Shimomura, O.; Yampolsky, I.V.

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LOCALIZATION OF ACTIVE-SITE OF ENZYME, BACTERIAL LUCIFERASE, USING 2-QUANTUM AFFINITY MODIFICATION [Текст] / L. Z. BENIMETSKAYA [и др.] // Dokl. Akad. Nauk. - 1994. - Vol. 336, Is. 1. - С. 114-117. - Cited References: 10 . - ISSN 0869-5652

РУБ Multidisciplinary Sciences

Держатели документа:
RUSSIAN ACAD SCI,SIBERIAN DIV,INST BIOPHYS,KRASNOYARSK,RUSSIA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
BENIMETSKAYA, L.Z.; KOZIONOV, A.L.; NOVOZHILOV, S.Y.; PETUSHKOV, V.N.; RODIONOVA, N.S.; SHTOKMAN, M.I.

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Hydrogen-bond networks between the C-terminus and Arg from the first alpha-helix stabilize photoprotein molecules [Text] / E. V. Eremeeva [et al.] // Photochem. Photobiol. Sci. - 2014. - Vol. 13, Is. 3. - P541-547, DOI 10.1039/c3pp50369k. - Cited References: 22. - The work was supported by RFBR grant 12-04-00753-a, by the Program of the Government of Russian Federation "Measures to Attract Leading Scientists to Russian Educational Institutions" (grant 11.G34.31.0058). . - ISSN 1474-905X. - ISSN 1474-9092

РУБ Biochemistry & Molecular Biology + Biophysics + Chemistry, Physical
Рубрики:
GREEN FLUORESCENT PROTEIN
   CA2+-REGULATED PHOTOPROTEIN
   BIOLUMINESCENT IMMUNOASSAY
   COELENTERAZINE BINDING
   ANGSTROM RESOLUTION
   ENERGY-TRANSFER
   FUSION PROTEIN
   APO-OBELIN
   AEQUORIN
   EXPRESSION
Аннотация: Previous studies have stated that aequorin loses most of its bioluminescence activity upon modification of the C-terminus, thus limiting the production of photoprotein fusion proteins at its N-terminus. In the present work, we investigate the importance of the C-terminal proline and the hydrogen bonds it forms for photoprotein active complex formation, stability and functional activity. According to the crystal structures of obelin and aequorin, two Ca2+-regulated photoproteins, the carboxyl group of the C-terminal Pro forms two hydrogen bonds with the side chain of Arg21 (Arg15 in aequorin case) situated in the first a-helix. Whereas, deletion or substitution of the C-terminal proline could noticeably change the bioluminescence activity, stability or the yield of an active photoprotein complex. Therefore, modifications of the first alpha-helix Arg has a clear destructive effect on the main photoprotein properties. A C-terminal hydrogen-bond network is proposed to be important for the stability of photoprotein molecules towards external disturbances, when taking part in the formation of locked protein conformations and isolation of coelenterazine-binding cavities.

WOS
Держатели документа:
[Eremeeva, Elena V.
Burakova, Ludmila P.
Krasitskaya, Vasilisa V.
Kudryavtsev, Alexander N.
Frank, Ludmila A.] Russian Acad Sci, Inst Biophys, Siberian Branch, Photobiol Lab, Krasnoyarsk 660036, Russia
[Eremeeva, Elena V.
Burakova, Ludmila P.
Krasitskaya, Vasilisa V.
Kudryavtsev, Alexander N.
Shimomura, Osamu
Frank, Ludmila A.] Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Lab Bioluminescence Biotechnol, Krasnoyarsk 660041, Russia
[Shimomura, Osamu] Marine Biol Lab, Woods Hole, MA 02543 USA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Eremeeva, E.V.; Burakova, L.P.; Krasitskaya, V.V.; Kudryavtsev, A.N.; Shimomura, O...; Frank, L.A.; RFBR [12-04-00753-a]; Government of Russian Federation [11.G34.31.0058]

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10.

Modification and comparative study of commercial nanodiamonds / A. P. Puzyr, A. E. Burov, V. S. Bondar // Fullerenes Nanotubes Carbon Nanostruct. - 2015. - Vol. 23, Is. 2. - P93-97, DOI 10.1080/1536383X.2013.794338 . - ISSN 1536-383X
Кл.слова (ненормированные):
colloidal stability -- detonation nanodiamond -- surface modification -- Detonation -- Powders -- Stability -- Surface treatment -- Suspensions (fluids) -- Aqueous suspensions -- Colloidal Stability -- Comparative studies -- Detonation nanodiamond -- Elemental compositions -- Particles size distribution -- Sedimentation stability -- Surface impurities -- Nanodiamonds
Аннотация: Detonation nanodiamond powders produced by different manufacturers were surface modified to reduce the amount of surface impurities and impart to the particles a high colloidal stability in aqueous suspensions. Modified DNDs (MDNDs) were separated into fractions and evaluated by the zeta potential, particles size distribution and elemental composition. MDNDs retain colloidal stability after the hydrosols are dried and the resulting powders are resuspended. Sedimentation stability and fraction composition are proposed as parameters for standardization of commercial nanodiamonds. © 2015 Copyright © Taylor & Francis Group, LLC.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, Krasnoyarsk, Russian Federation
Special Design-Technology Bureau nauka KSC SB RAS, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Puzyr, A.P.; Burov, A.E.; Bondar, V.S.

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11.

INACTIVATION OF BACTERIAL LUCIFERASES BY N-ETHYLMALEIMIDE [Text] / T. P. SANDALOVA, N. A. TYULKOVA // Biochem.-Moscow. - 1992. - Vol. 57, Is. 6. - P. 552-558. - Cited References: 21 . - ISSN 0006-2979

РУБ Biochemistry & Molecular Biology
Рубрики:
AMINO-ACID SEQUENCE
   NUCLEOTIDE-SEQUENCE
   REACTIVE SULFHYDRYL
   PHOTOBACTERIUM-LEIOGNATHI
   VIBRIO-HARVEYI
   BIOLUMINESCENCE
   SUBUNIT
   REGION
   GENE
Кл.слова (ненормированные):
LUCIFERASE -- N-ETHYLMALEIMIDE
Аннотация: The kinetics of inactivation of luciferases from four species of luminescent bacteria by the thiol reagent N-ethylmaleimide were investigated The dependencies of inactivation on ionic strength differed among the enzymes. Increasing the molarity of the buffer increased the rate of inactivation of all luciferases except that of Vibrio harveyi. Modification of Photobacterium phosphoreum luciferase decreased the maximal intensity of bioluminescence, whereas modification of Photobacterium leiognathi and Vibrio fischeri luciferases in high ionic strength buffers decreased the maximal intensity of bioluminescence and changed the luminescence decay rate constant. High ionic strength apparently alters the conformational states of the luciferases.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
SANDALOVA, T.P.; TYULKOVA, N.A.

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12.

ELECTROPHORETIC BEHAVIOR OF HYDROSOLS OF ULTRADISPERSE DIAMOND AND MODIFICATION OF ITS SURFACE [Text] / G. A. CHIGANOVA, V. A. BONDAR, A. S. CHIGANOV // COLLOID JOURNAL OF THE RUSSIAN ACADEMY OF SCIENCES. - 1993. - Vol. 55, Is. 5. - P. 774-775. - Cited References: 5 . - ISSN 1061-933X

РУБ Chemistry, Physical

Аннотация: The method of macro-electrophoresis is used to determine values of the zeta-potential of particles of ultradisperse diamond in solutions of AlCl3. It is shown that an increase in the concentration of aluminum ions to 0.5.10(-4) M leads to a decrease in the negative charge of the diamond particles; the dispersions coagulate in more concentrated solutions of AlCl3. Chemical modification of the surface of ultradisperse diamond by aluminum ions is used to obtain stable hydrosols with positively charged diamond particles.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
CHIGANOVA, G.A.; BONDAR, V.A.; CHIGANOV, A.S.

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13.

Physicochemical properties of Al2O3 powder produced by explosive synthesis [Text] / A. A. Bukaemskii, A. G. Beloshapko, A. P. Puzyr // Combust. Explos. - 2000. - Vol. 36, Is. 5. - P. 660-666, DOI 10.1007/BF02699530. - Cited References: 18 . - ISSN 0010-5082

РУБ Thermodynamics + Energy & Fuels + Engineering, Multidisciplinary + Engineering, Chemical + Materials Science, Multidisciplinary

Аннотация: The phase, dispersive, and morphological features of an aluminum oxide powder produced by explosive synthesis are examined experimentally. It is shown that the particle-size distribution has three distinct maxima, which are due to different combustion regimes. The relationship between the sizes and morphology of the starting powder and the product is determined. Considerable attention is given to a study of the ultrafine fraction of the product powder. The ultrafine particles are shown to have a regular spherical shape, and sinters are not revealed. Besides spherical particles, the synthesized powder contains faceted crystals. X-ray phase analysis of the ultrafine fraction of the synthesized powders shows that this fraction consists only of the metastable phases of the oxide - the delta- or oxynitride modifications. The delta -modification differs from that described in the literature.

WOS
Держатели документа:
Krasnoyarsk State Univ, Inst Physicotech, Krasnoyarsk 660036, Russia
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bukaemskii, A.A.; Beloshapko, A.G.; Puzyr, A.P.

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14.

Oxygen and ammonia plasma treatment of poly(3-hydroxybutyrate) films for controlled surface zeta potential and improved cell compatibility [Text] / D. S. Syromotina [et al.] // Mater. Lett. - 2016. - Vol. 163. - P277-280, DOI 10.1016/j.matlet.2015.10.080. - Cited References:10. - The authors thank Mr. T.M Mukhametkaliyev, Mrs. A.A. Sharonova and Dr. A. Wittmar for their assistance with the experiments. This research was supported by the Russian President's Stipend SP-6664.2013.4, Ministry for Education and Science Grant MK-485.2014.8, the State order NAUKA #11.1359.2014/K, and the State budget allocated to the fundamental research at the Russian Academy of Sciences (project No 01201351505). . - ISSN 0167-577X. - ISSN 1873-4979

РУБ Materials Science, Multidisciplinary + Physics, Applied

Кл.слова (ненормированные):
Plasma treatment -- Polymer -- Cell adhesion -- Surface modification
Аннотация: The oxygen and ammonia radio-frequency (RF) plasma treatment of poly(3-hydroxybutyrate) P3HB films was performed. We revealed significant changes in the topography, a decrease in the surface zeta potential from -63 to -75 mV after the oxygen-plasma treatment and an increase after ammonia plasma treatment from -63 to -45 mV at a pH of 7.4. Investigations into the NIH 3T3 fibroblast adhesion and growth demonstrated the best cell vitality and a higher cell number for the ammonia plasma treatment at 150W. (C) 2015 Elsevier B.V. All rights reserved.

WOS
Держатели документа:
Natl Res Tomsk Polytech Univ, Tomsk 634050, Russia.
Fraunhofer Inst Interfacial Engn & Biotechnol IGB, D-70569 Stuttgart, Germany.
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.
Univ Duisburg Essen, Inorgan Chem, D-45117 Essen, Germany.
Univ Duisburg Essen, Ctr Nanointegrat Duisburg Essen CeNIDE, D-45117 Essen, Germany.
Univ Duisburg Essen, Tech Chem 2, D-45141 Essen, Germany.
Univ Duisburg Essen, Ctr Nanointegrat Duisburg Essen CeNIDE, D-45141 Essen, Germany.

Доп.точки доступа:
Syromotina, D. S.; Surmenev, R. A.; Surmeneva, M. A.; Boyandin, A. N.; Epple, M.; Ulbricht, M.; Oehr, C.; Volova, T. G.; Russian President's Stipend [SP-6664.2013.4]; Ministry for Education and Science Grant [MK-485.2014.8]; State order NAUKA [11.1359.2014/K]; State budget [01201351505]

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15.

Stable isotope fractionation of fatty acids of Daphnia fed laboratory cultures of microalgae / M. I. Gladyshev [et al.] // Limnologica. - 2016. - Vol. 56. - P23-29, DOI 10.1016/j.limno.2015.12.001 . - ISSN 0075-9511
Кл.слова (ненормированные):
Compound-specific isotope analysis -- Fatty acids -- Food webs -- Stable isotope fractionation -- comparative study -- crustacean -- fatty acid -- food web -- isotopic analysis -- isotopic fractionation -- microalga -- stable isotope -- Chlorella vulgaris -- Cryptomonas sp. -- Daphnia -- Daphnia galeata
Аннотация: We tested a comparatively new method of tracing of natural food webs, compound-specific isotope analysis (CSIA) of fatty acids (FA), using laboratory culture of Daphnia galeata fed Chlorella vulgaris and Cryptomonas sp. In general, Daphnia had significantly lighter carbon stable isotope composition of most fatty acids, including essential, than those of their food, microalgae. Thus, our results did not support the pivotal premise of the FA-CSIA application for food web analysis, i.e., transmitting the isotope 'signal' of essential FAs to consumers from their food without any modification. Moreover, the values of isotope fractionation of particular FAs in the consumer relative to its food were not constant, but varied from 1.35‰ to 7.04‰. The different isotope fractionation (depletion) values of diverse FAs in consumer were probably caused by different processes of their synthesis, catabolism and assimilation. More work is evidently to be done for correct interpretation of results of FA-CSIA during field studies for tracing of natural food webs. © 2015 Elsevier GmbH.

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Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation
Siberian Federal University, Svobodny av. 79, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Gladyshev, M. I.; Makhutova, O. N.; Kravchuk, E. S.; Anishchenko, O. V.; Sushchik, N. N.

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16.

Ni2+-zeolite/ferrosphere and Ni2+-silica/ferrosphere beads for magnetic affinity separation of histidine-tagged proteins / T. A. Vereshchagina [et al.] // Dalton Trans. - 2016. - Vol. 45, Is. 4. - P1582-1592, DOI 10.1039/c5dt03827h . - ISSN 1477-9226
Кл.слова (ненормированные):
Alkalinity -- Amino acids -- Chemical modification -- Fly ash -- Hydrothermal synthesis -- Ion exchange -- Magnetic separation -- Magnetism -- Proteins -- Silica -- Zeolites -- Conventional methods -- Core shell structure -- Green fluorescent protein -- Histidine-tagged proteins -- Hydrothermal treatments -- Magnetic affinity -- Mesoporous Silica -- Sorption capacities -- Nickel
Аннотация: Magnetic Ni2+-zeolite/ferrosphere and Ni2+-silica/ferrosphere beads (Ni-ferrosphere beads - NFB) of a core-shell structure were synthesized starting from coal fly ash ferrospheres having diameters in the range of 0.063-0.050 mm. The strategy of NFB fabrication is an oriented chemical modification of the outer surface preserving the magnetic core of parent beads with the formation of micro-mesoporous coverings. Two routes of ferrosphere modification were realized, such as (i) hydrothermal treatment in an alkaline medium resulting in a NaP zeolite layer and (ii) synthesis of micro-mesoporous silica on the glass surface using conventional methods. Immobilization of Ni2+ ions in the siliceous porous shell of the magnetic beads was carried out via (i) the ion exchange of Na+ for Ni2+ in the zeolite layer or (ii) deposition of NiO clusters in the zeolite and silica pores. The final NFB were tested for affinity in magnetic separation of the histidine-tagged green fluorescent protein (GFP) directly from a cell lysate. Results pointed to the high affinity of the magnetic beads towards the protein in the presence of 10 mM EDTA. The sorption capacity of the ferrosphere-based Ni-beads with respect to GFP was in the range 1.5-5.7 mg cm-3. © The Royal Society of Chemistry.

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Держатели документа:
Institute of Chemistry and Chemical Technology SB RAS, 50/24 Akademgorodok, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, 50/50 Akademgorodok, Krasnoyarsk, Russian Federation
Siberian Federal University, 79 Svobodnyi Avenue, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Vereshchagina, T. A.; Fedorchak, M. A.; Sharonova, O. M.; Fomenko, E. V.; Shishkina, N. N.; Zhizhaev, A. M.; Kudryavtsev, A. N.; Frank, L. A.; Anshits, A. G.

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17.

Surface wettability and energy effects on the biological performance of poly-3-hydroxybutyrate films treated with RF plasma / D. S. Syromotina [et al.] // Mater. Sci. Eng. C. - 2016. - Vol. 62. - P450-457, DOI 10.1016/j.msec.2016.01.075 . - ISSN 0928-4931
Кл.слова (ненормированные):
Plasma -- Polar component -- poly-3-hydroxybutyrate membranes -- Surface energy -- Surface treatment -- Ammonia -- Biodegradable polymers -- Cell adhesion -- Contact angle -- Free energy -- Interfacial energy -- Plasma applications -- Plasmas -- Surface chemistry -- Surface properties -- Surface roughness -- Surface topography -- Wetting -- Biological performance -- Crystalline structure -- Polar components -- Poly-3-hydroxybutyrate -- Surface free energy -- Surface roughness changes -- Surface wettability -- Water contact angle -- Surface treatment
Аннотация: The surface properties of poly-3-hydroxybutyrate (P3HB) membranes were modified using oxygen and an ammonia radio-frequency (RF, 13.56 MHz) plasma. The plasma treatment procedures used in the study only affected the surface properties, including surface topography, without inducing any significant changes in the crystalline structure of the polymer, with the exception being a power level of 250 W. The wettability of the modified P3HB surfaces was significantly increased after the plasma treatment, irrespective of the treatment procedure used. It was revealed that both surface chemistry and surface roughness changes caused by the plasma treatment affected surface wettability. A treatment-induced surface aging effect was observed and resulted in an increase in the water contact angle and a decrease in the surface free energy. However, the difference in the water contact angle between the polymers that had been treated for 4 weeks and the untreated polymer surfaces was still significant. A dependence between cell adhesion and proliferation and the polar component of the surface energy was revealed. The increase in the polar component after the ammonia plasma modification significantly increased cell adhesion and proliferation on biodegradable polymer surfaces compared to the untreated P3HB and the P3HB modified using an oxygen plasma. © 2016 Elsevier B.V.

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Держатели документа:
Department of Experimental Physics, National Research Tomsk Polytechnic University, Tomsk, Russian Federation
Fraunhofer Institute for Interfacial Engineering and Biotechnology, IGB, Stuttgart, Germany
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, 50/50 Akademgorodok, Krasnoyarsk, Russian Federation
School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodny pr., Krasnoyarsk, Russian Federation
Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Essen, Germany
Technical Chemistry II and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Essen, Germany

Доп.точки доступа:
Syromotina, D. S.; Surmenev, R. A.; Surmeneva, M. A.; Boyandin, A. N.; Nikolaeva, E. D.; Prymak, O.; Epple, M.; Ulbricht, M.; Oehr, C.; Volova, T. G.

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18.

The vertical distribution of soil organic matter predicted by a simple continuous model of soil organic matter transformations / S. I. Bartsev, A. A. Pochekutov // Ecol. Model. - 2016. - Vol. 328. - P95-98, DOI 10.1016/j.ecolmodel.2016.02.020 . - ISSN 0304-3800
Кл.слова (ненормированные):
Continuous model of soil -- Soil organic matter -- Soil profile -- Vertical soil organic matter distribution
Аннотация: New modification of the simple continuous model of soil organic matter (SOM) transformation is proposed. The modification allows to convert a SOM distribution over humification rate to a SOM distribution over depth and vice versa. Qualitative correspondence of calculated curve patterns of SOM vertical distributions to observed ones for different soil types is shown. © 2016 Elsevier B.V.

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Держатели документа:
Institute of Biophysics SB RAS, Krasnoyarsk, Russian Federation
Institute of Fundamental Biology and Biotechnology of Siberian Federal University, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Bartsev, S. I.; Pochekutov, A. A.

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19.

Mutants of Ca2+-regulated Photoprotein Obelin for Site-specific Conjugation / V. V. Krasitskaya [et al.] // Photochem. Photobiol. - 2017. - Vol. 93, Is. 2. - P553-557, DOI 10.1111/php.12712 . - ISSN 0031-8655
Аннотация: Color variants of Ca2+-regulated photoprotein obelin were shown to be an important tool for dual-analyte binding assay. To provide site-directed conjugation with biospecific molecules, several obelin color mutants carrying unique cysteine residues were obtained and characterized for their novel properties. A pair of obelins Y138F,A5C and W92F,H22E,D12C was found to be most suitable (in terms of high bioluminescent activity and stability) as reporters in simultaneous assay of two targets in a sample. Availability of SH-groups, accessible for chemical modification, essentially simplifies the synthesis of biospecific conjugates, increases their yield and conserves obelins' bioluminescence activity. Conjugates with immunoglobulin and oligonucleotide were produced and successfully applied in single nucleotide polymorphism genotyping. © 2017 The American Society of Photobiology

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Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Krasitskaya, V. V.; Burakova, L. P.; Komarova, A. A.; Bashmakova, E. E.; Frank, L. A.

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20.

Active mixing of immobilised enzymatic system in microfluidic chip / K. A. Lukyanenko [et al.] // Micro Nano Lett. - 2017. - Vol. 12, Is. 6. - P377-381, DOI 10.1049/mnl.2016.0646. - Cited References:17. - The research was supported by the grant of the Russian Science Foundation (project no. 15-19-10041). . - ISSN 1750-0443

РУБ Nanoscience & Nanotechnology + Materials Science, Multidisciplinary
Рубрики:
POLY(METHYL METHACRYLATE)
   SURFACE MODIFICATION
   POINT
   DEVICES
   PMMA
Аннотация: Parameters for sample introduction, dried reagents dissolution and mixing with sample for bienzyme system NAD(H):FMN-oxidoreductase and luciferase immobilised in microfluidic chip were successfully determined. Numerical simulations of reaction chamber geometry, flavin mononucleotide (FMN) escape from starch gel and mixing options were conducted to achieve higher sensitivity of bioluminescent reaction. Results of numerical simulations were verified experimentally. The active mixer for dried reagents was made from an electro-mechanical speaker's membrane which was connected to the input of the chip. Such a mixer provided better efficiency than a passive mixing, and it is simple enough for use in point-of-care devices with any systems based on immobilised enzymes in chips.

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Держатели документа:
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
ITMO Univ, St Petersburg 197101, Russia.
Inst Biophys SB RAS, Krasnoyarsk 660036, Russia.
Inst Analyt Instrumentat, St Petersburg 198095, Russia.

Доп.точки доступа:
Lukyanenko, Kirill A.; Belousov, Kirill I.; Denisov, Ivan A.; Yakimov, Anton S.; Esimbekova, Elena N.; Bukatin, Anton S.; Evstrapov, Anatoly A.; Belobrov, Peter I.; Russian Science Foundation [15-19-10041]

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