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Physicochemical conditions of seasonal carbonate precipitation in Shira lake (Khakasia) / G. A. Tret'Yakov [et al.] // Doklady Earth Sciences. - 2012. - Vol. 446, Is. 1. - P1099-1101, DOI 10.1134/S1028334X12090152 . - ISSN 1028-334X
Кл.слова (ненормированные):
carbonate sediment -- lacustrine deposit -- physicochemical property -- precipitation (chemistry) -- Khakassia -- Lake Shira -- Russian Federation

Scopus
Держатели документа:
Sobolev Institute of Geology and Mineralogy, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russian Federation
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tret'Yakov, G.A.; Kalugin, I.A.; Dar'In, A.V.; Rogozin, D.Yu.; Degermendzhi, A.G.

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Vertical stratification of physical, chemical and biological components in two saline lakes Shira and Shunet (South Siberia, Russia) / A. G. Degermendzhy [et al.] // Aquatic Ecology. - 2010. - Vol. 44, Is. 3. - P619-632, DOI 10.1007/s10452-010-9336-6 . - ISSN 1386-2588
Кл.слова (ненормированные):
Ciliates -- Cryptomonas -- Fishless lakes -- Gammarus -- Mathematical modelling -- Meromictic lakes -- Phytoplankton -- Stratification -- Sulphur bacteria -- amphipod -- bacterium -- biomass -- ciliate -- ecosystem modeling -- flagellate -- meromictic lake -- microbial community -- numerical model -- physicochemical property -- phytoplankton -- population density -- saline lake -- salinity -- stratification -- thermocline -- trophic interaction -- vertical profile -- zooplankton -- Khakassia -- Lake Shira -- Lake Shunet -- Russian Federation -- Siberia -- Amphipoda -- Bacteria (microorganisms) -- Ciliophora -- Copepoda -- Cryptomonas -- Cryptomonas sp. -- Gammaridae -- Gammarus -- Gammarus lacustris -- Phytomastigophorea -- Protista -- Rotifera
Аннотация: A feature of meromictic lakes is that several physicochemical and biological gradients affect the vertical distribution of different organisms. The vertical stratification of physical, chemical and biological components in saline, fishless meromictic lakes Shira and Shunet (Siberia, Russia) is quite different mainly because both mean depth and maximum depth of lakes differ as well as their salinity levels differ. The chemocline of the Lake Shira, as in many meromictic lakes, is inhabited by bacterial community consisting of purple sulphur and heterotrophic bacteria. As the depth of the chemocline is variable, the bacterial community does not attain high densities. The mixolimnion in Lake Shira, which is thermally stratified in summer, also creates different habitat for various species. The distribution of phytoplankton is non-uniform with its biomass peak in the metalimnion. The distribution of zooplankton is also heterogeneous with rotifers and juvenile copepods inhabiting the warmer epilimnion and older copepods found in the cold but oxic hypolimnion. The amphipod Gammarus lacustris which can be assigned to the higher trophic link in the fishless lake's ecosystem, such as Lake Shira, is also distributed non-uniformly, with its peak density generally observed in the thermocline region. The chemocline in Lake Shunet is located at the depth of 5 m, and unlike in Lake Shira, due to a sharp salinity gradient between the mixolimnion and monimolimnion, this depth is very stable. The mixolimnion in Lake Shunet is relatively shallow and the chemocline is inhabited by (1) an extremely dense bacterial community; (2) a population of Cryptomonas sp.; and (3) ciliate community comprising several species. As the mixolimnion of Lake Shunet is not thermally stratified for long period, the phytoplankton and zooplankton populations are not vertically stratified. The gammarids, however, tend to concentrate in a narrow layer located 1-2 m above the chemocline. We believe that in addition to vertical inhomogeneities of both physicochemical parameters, biological and physical factors also play a role in maintaining these inhomogeneities. We conclude that the stratified distributions of the major food web components will have several implications for ecosystem structure and dynamics. Trophic interactions as well as mass and energy flows can be significantly impacted by such heterogeneous distributions. Species spatially separated even by relatively short distances, say a few centimetres will not directly compete. Importantly, we demonstrate that not only bacteria, phytoflagellates and ciliate tend to concentrate in thin layers but also larger-sized species such Gammarus (amphipods) can also under certain environmental conditions have stratified distribution with maxima in relatively thin layer. As the vertical structure of the lake ecosystem is rather complex in such stratified lakes as ours, the strategy of research, including sampling techniques, should consider potentially variable and non-homogeneous distributions. В© 2010 The Author(s).

Scopus
Держатели документа:
Institute of Biophysics SB RAS, 660036 Krasnoyarsk, Akademgorodok, Russian Federation
Siberian Federal University, Svobodnyi 79, 660041 Krasnoyarsk, Russian Federation
Netherlands Environmental Assessment Agency (PBL), P.O. Box 303, 3720 AH Bilthoven, Netherlands
Department of Aquatic Ecology, Netherlands Institute of Ecology (NIOO-KNAW), 3631 AC Nieuwersluis, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Degermendzhy, A.G.; Zadereev, E.S.; Rogozin, D.Y.; Prokopkin, I.G.; Barkhatov, Y.V.; Tolomeev, A.P.; Khromechek, E.B.; Janse, J.H.; Mooij, W.M.; Gulati, R.D.

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Some generalizations based on stratification and vertical mixing in meromictic Lake Shira, Russia, in the period 2002-2009 / D. Y. Rogozin [et al.] // Aquatic Ecology. - 2010. - Vol. 44, Is. 3. - P485-496, DOI 10.1007/s10452-010-9328-6 . - ISSN 1386-2588
Кл.слова (ненормированные):
1-D model -- Meromixis -- Mixolimnion -- Oxic-anoxic interface -- Thermocline -- Weather conditions -- brackish water -- meromictic lake -- numerical model -- one-dimensional modeling -- overturn -- physicochemical property -- salinity -- seasonality -- stratification -- temperate environment -- vertical mixing -- water temperature -- weather -- Khakassia -- Lake Shira -- Russian Federation
Аннотация: In a brackish, temperate, 24-m-deep Lake Shira, the profiles of salinity, temperature, oxygen and sulfide concentrations were measured on a seasonal basis from 2002 to 2009. The lake was shown to be meromictic with autumnal overturn restricted to mixolimnion. The depth of mixolimnion and position of oxic-anoxic interface varied annually. The spring mixing processes contribute to the formation of mixolimnion in autumn. The exceptionally windy spring of 2007 caused the deepening of mixolimnion in the winter of 2008. The winter position of oxic-anoxic interface was affected by the position of lower boundary of mixolimnion in all winters. The salinity in the winter mixolimnion increased compared with the autumn because of freezing out of salts from the upper water layers meters during ice formation and their dissolution in water below. The profiles of salinity and temperature were simulated by the mathematical 1-D model of temperature and salinity conditions taking into account ice formation. The simulated profiles generally coincided with the measured ones. The coincidence implies that simplified one-dimensional model can be applied to roughly describe salinity and density profiles and mixing behavior of Lake Shira. В© 2010 The Author(s).

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok 50-50, 660036 Krasnoyarsk, Russian Federation
Siberian Federal University, Svobodny 79, 660071 Krasnoyarsk, Russian Federation
Institute of Computational Modeling of Siberian Branch of Russian Academy of Sciences, Akademgorodok 50-50, 660036 Krasnoyarsk, Russian Federation
The Netherlands Institute of Ecology, Center for Aquatic Ecology, Nieuwersluis, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Rogozin, D.Y.; Genova, S.N.; Gulati, R.D.; Degermendzhy, A.G.

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Self-restoration as fundamental property of CES providing their sustainability / I. I. Gitelson, A. G. Degermendzhy, E. K. Rodicheva // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1641-1648, DOI 10.1016/S0273-1177(03)00111-X . - ISSN 0273-1177
Кл.слова (ненормированные):
Biocatalysts -- Ecosystems -- Genes -- Life support systems (spacecraft) -- Radiation damage -- Cell populations -- Space flight -- space shuttle -- article -- biological model -- biomass -- cell division -- Chlorella -- cytology -- growth, development and aging -- microclimate -- radiation exposure -- radiation response -- ultraviolet radiation -- Biomass -- Cell Division -- Chlorella -- Dose-Response Relationship, Radiation -- Ecological Systems, Closed -- Life Support Systems -- Models, Biological -- Ultraviolet Rays
Аннотация: Sustainability is one of the most important criteria and evaluation of human life support systems intended for use during long space flights. The common feature of biological and physicochemical life support systems is that basically they are both catalytic. But there are two fundamental properties distinguishing biological systems: 1) they are auto-catalytic: their catalysts - enzymes of protein nature - are continuously reproduced when the system functions; 2) the program of every process performed by enzymes and the program of their reproduction are inherent in the biological system itself - in the totality of genomes of the species involved in the functioning of the ecosystem. Actually, one cell with the genome capable of the phenotypic realization is enough for the self-restoration of the function performed by the cells of this species in the ecosystem. The continuous microalgal culture of Chlorella vulgaris was taken to investigate quantitatively the process of self-restoration in unicellular algae population. Based on the data obtained, we proposed a mathematical model of the restoration process in a cell population that has suffered an acute radiation damage. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, SB RAS, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, I.I.; Degermendzhy, A.G.; Rodicheva, E.K.

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The physicochemical properties of polyhydroxyalkanoates with different chemical structures / T. G. Volova [et al.] // Polymer Science - Series A. - 2013. - Vol. 55, Is. 7. - P427-437, DOI 10.1134/S0965545X13070080 . - ISSN 0965-545X
Кл.слова (ненормированные):
Amorphous and crystalline phasis -- Degrees of crystallinity -- Physicochemical property -- Poly(3-hydroxybutirate) -- Polyhydroxyalkanoate copolymer -- Temperature characteristic -- Thermal degradation temperatures -- Thermoplastic materials -- Copolymers -- Monomers -- Thermoplastic elastomers -- Structure (composition)
Аннотация: A set of polyhydroxyalkanoates are synthesized, and a comparative study of their physicochemical properties is performed. The molecular masses and polydispersities of polyhydroxyalkanoates are found to be independent of their chemical structures. It is shown that the temperature characteristics and degrees of crystallinity of polyhydroxyalkanoates are affected by the chemical compositions of the monomers and their quantitative contents in the polymers. The incorporation of 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate units into the chain of poly(3-hydroxybutyrate) decreases its melting point and thermal degradation temperature relative to these parameters of a homogeneous poly(3-hydroxybutyrate) sample (175 В± 5 C and 275 В± 5 C, respectively). The higher the content of the second monomer units in the poly(3-hydroxybutirate) chain, the greater the changes. The degrees of crystallinity of polyhydroxyalkanoate copolymers are generally lower than that of poly(3-hydroxybutyrate) (75 В± 5%). The effect on the ratio of the amorphous and crystalline phases of the copolymer samples becomes more pronounced in the series 3-hydroxy-valerate-3-hydroxyhexanoate-4- hydroxybutyrate. The prepared samples exhibit different properties ranging from rigid thermoplastic materials to engineering elastomers. В© 2013 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50, str. 50, Krasnoyarsk 660036, Russian Federation
Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Svobodnyi pr. 79, Krasnoyarsk 660041, Russian Federation
Siberian State Technological University, pr. Mira 82, Krasnoyarsk 660049, Russian Federation
Kirensky Institute of Physics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50, str. 38, Krasnoyarsk 660036, Russian Federation
Massachusetts Institute of Technology, Cambridge, MA, United States : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Zhila, N.O.; Shishatskaya, E.I.; Mironov, P.V.; Vasil'Ev, A.D.; Sukovatyi, A.G.; Sinskey, A.J.

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Physiological-biochemical properties and the ability to synthesize polyhydroxyalkanoates of the glucose-utilizing strain of the hydrogen bacterium Ralstonia eutropha B8562 / T. G. Volova [et al.] // Applied Microbiology and Biotechnology. - 2006. - Vol. 73, Is. 3. - P429-433, DOI 10.1007/s00253-006-0460-0 . - ISSN 0175-7598
Кл.слова (ненормированные):
Biochemistry -- Carbon -- Genetic engineering -- Glucose -- Hydrogen -- Polyesters -- Cultural properties -- Glucose-utilizing mutant strain -- Nitrogen deficiency -- Polyhydroxyalkanoate (PHA) -- Bacteria -- 3 hydroxybutyric acid -- carbon dioxide -- fructose -- glucose -- hydrogen -- monomer -- polyhydroxyalkanoic acid -- sugar -- bacterium -- carbon -- comparative study -- energy -- ester -- experimental study -- glucose -- hydrogen -- physicochemical property -- article -- bacterial strain -- bacterium culture -- bacterium mutant -- biomass -- chemical analysis -- Cupriavidus necator -- energy consumption -- genetic analysis -- nonhuman -- synthesis -- Bacteria (microorganisms) -- Cupriavidus necator
Аннотация: Physiological-biochemical, genetic, and cultural properties of the glucose-utilizing mutant strain Ralstonia eutropha B8562 have been compared with those of its parent strain R. eutropha B5786. It has been shown that growth characteristics of the strain cultured on glucose as the sole carbon and energy source are comparable with those of the parent strain. Strain B8562 is characterized by high polyhydroxyalkanoate (PHA) yields on different carbon sources (CO2, fructose, and glucose). PHA accumulation in the strain batch cultured on glucose under nitrogen deficiency reaches 90 %. The major monomer in the PHA is ?-hydroxybutyric acid (more than 99 mol %); the identified minor components are ?-hydroxyvaleric acid (0.25-0.72 mol %) and ?-hydroxyhexanoic acid (0.08-1.5 mol %). The strain is a promising PHA producer on available sugar-containing media with glucose. В© Springer-Verlag 2006.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russian Federation
Krasnoyarsk State University, Svobodny av. 79, 660041 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Trusova, M.Y.; Kalacheva, G.S.; Kozhevnicov, I.V.

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A hybrid PHB-hydroxyapatite composite for biomedical application: Production, in vitro and in vivo investigation / E. I. Shishatskaya, I. A. Khlusov, T. G. Volova // Journal of Biomaterials Science, Polymer Edition. - 2006. - Vol. 17, Is. 5. - P481-498, DOI 10.1163/156856206776986242 . - ISSN 0920-5063
Кл.слова (ненормированные):
Biocompatibility -- Hydroxyapatite (HA) -- PHB-hydroxyapatite composite -- Polyhydroxyalkanoate (PHA) -- Polyhydroxybutyrate (P(3HB)) -- Properties -- Biocompatibility -- Differential thermal analysis -- Electron microscopy -- Free energy -- Interfacial energy -- Physical properties -- Surface properties -- X ray analysis -- Biomedical application -- Physicochemical properties -- Polyhydroxyalkanoate (PHA) -- Polyhydroxybutyrate (PHB) -- Hydroxyapatite -- hydroxyapatite -- poly(3 hydroxybutyric acid) -- polymer -- biomaterial -- hydroxybutyric acid -- adhesion -- animal cell -- animal tissue -- article -- biomedicine -- bone marrow cell -- cell differentiation -- cell growth -- chemical structure -- composite material -- controlled study -- crystallization -- decomposition -- electron microscopy -- in vitro study -- in vivo study -- melting point -- mouse -- nonhuman -- ossification -- osteoblast -- physical chemistry -- priority journal -- rat -- strength -- structure analysis -- surface property -- synthesis -- temperature measurement -- thermal analysis -- tissue engineering -- wettability -- animal -- biomechanics -- bioremediation -- bone prosthesis -- cattle -- cell culture -- chemistry -- cytology -- differential scanning calorimetry -- drug effect -- human -- materials testing -- prostheses and orthoses -- scanning electron microscopy -- standard -- Wistar rat -- Murinae -- Animals -- Biocompatible Materials -- Biodegradation, Environmental -- Biomechanics -- Bone Substitutes -- Cattle -- Cells, Cultured -- Differential Thermal Analysis -- Durapatite -- Humans -- Hydroxybutyrates -- Materials Testing -- Microscopy, Electron, Scanning -- Osteoblasts -- Prostheses and Implants -- Rats -- Rats, Wistar -- Surface Properties
Аннотация: Samples of a hybrid composite of polyhydroxybutyrate (PHB), a biodegradable polyester, and hydroxyapatite (HA), with different PHB/HA ratios, have been prepared using mechanical-physical method. Electron microscopy, X-ray structure analysis and differential thermal analysis have been used to investigate the structure and physicochemical properties of the composite, depending on the PHB/HA ratio. The properties of the surface of the HA-loaded composite are significantly different from those of the pure polymer. As the HA percentage in the composite increases, free interface energy, the cohesive force, i.e., the strength of the adhesive bond between the composite surface and the water phase, and surface wettability increase. The HA percentage of the composite does not influence its melting temperature, but affects the temperature for the onset of decomposition: as the HA content increases from 0 to 10% (w/w), Td decreases from 260В°C to 225В°C. The degree of crystallinity of PHB/HA increases from 77% to 89% with an increase in the HA fraction from 10% to 50%. Functional properties of the composites have been investigated in vitro and in vivo. The best parameters of growth and differentiation of murine marrow osteoblasts are registered on PHB/HA samples containing 10% and 20% HA. In ectopic bone formation assay it has been proven that the hybrid PHB/HA composites can function as scaffolds and that bone tissue develops on their surface and in pores. В© VSP 2006.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 60036, Russian Federation
Tomsk State University, Tomsk 634021, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Khlusov, I.A.; Volova, T.G.

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Fatty acid content and composition of sediments from Siberian eutrophic water bodies: Implications for biodiesel production / N. N. Sushchik, A. Y. Kuchkina, M. I. Gladyshev // Water Research. - 2013. - Vol. 47, Is. 9. - P3192-3200, DOI 10.1016/j.watres.2013.03.031 . - ISSN 0043-1354
Кл.слова (ненормированные):
Biodiesel -- Bottom sediments -- Fatty acids -- Fuel properties -- Inland water bodies -- Lipids -- Biological conditions -- Bottom sediments -- Environmental characteristic -- Freshwater reservoirs -- Fuel properties -- Inland waters -- Organic matter source -- Transformation process -- Biodiesel -- Eutrophication -- Fatty acids -- Feedstocks -- Fuels -- Lipids -- Reservoirs (water) -- Sedimentology -- Thermochemistry -- Sediments -- biodiesel -- fresh water -- iodine -- organic matter -- biofuel -- bottom water -- combustion -- eutrophic environment -- eutrophication -- fatty acid -- lacustrine deposit -- physicochemical property -- reservoir -- transformation -- article -- estuary -- eutrophication -- fatty acid analysis -- lake -- lipid composition -- priority journal -- sediment -- Khakassia -- Krasnoyarsk [Krasnoyarsk (ADS)] -- Krasnoyarsk [Russian Federation] -- Lake Shira -- Russian Federation
Аннотация: We studied lipids and fatty acids (FA) in bottom sediments from four Siberian water bodies, Bugach, Lesnoi and Krasnoyarsk freshwater reservoirs and brackish Shira lake, that differed in physico-chemical and biological conditions. We considered the potential of the bottom sediments as a feedstock for biodiesel production and estimated properties of the obtained biodiesel as a fuel on the basis of FA composition. Contents of lipids and FA in the sediments moderately varied and were generally close to the reported data from lacustrine and estuarine systems. We confirmed that long-term eutrophication of a water body resulted in the lipid-rich bottom sediments that make them a feedstock for biodiesel production. Each of the studied water bodies had specific FA composition of sediments likely due to different organic matter sources and transformation processes. Despite these differences in FA profiles, calculated key parameters (cetane number, iodine number and heat of combustion) of biodiesel produced from all the studied sediments met the limits established by current biodiesel standards. Thus, the variation in the sediment FA composition due to environmental characteristics of a water body likely has no principal significance for fuel properties of the obtained biodiesel. В© 2013 Elsevier Ltd.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok 50/50, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, Svobodny av. 79, Krasnoyarsk 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sushchik, N.N.; Kuchkina, A.Y.; Gladyshev, M.I.

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An in situ method for the investigation of vertical distributions of zooplankton in lakes: Test of a two-compartment enclosure / A. P. Tolomeyev, Y. S. Zadereev // Aquatic Ecology. - 2005. - Vol. 39, Is. 1-2. - P181-188, DOI 10.1007/s10452-004-5732-0 . - ISSN 1386-2588
Кл.слова (ненормированные):
Anoxic hypolimnion -- Solar radiation -- Stratified lake -- Vertical migration -- Zooplankton -- biotic factor -- enclosure experiment -- lake ecosystem -- physicochemical property -- vertical distribution -- zooplankton -- Animalia -- Arctodiaptomus salinus -- Brachionus plicatilis
Аннотация: Two-section enclosures were designed for the investigation of the effect of various physicochemical and biological factors on vertical distribution of zooplankton in situ. The framework of the enclosure was a cylindrical polyethylene column without any partitions inside, in which the isolation of animals in different sections after in situ exposure was achieved by pinching the flexible central part of the column. Enclosures were tested at the brackish stratified meromictic Lake Shira (Russia, Khakasia). The absence of fish and carnivorous zooplankton in the lake suggests that the vertical distribution of zooplankton is mainly determined by physicochemical gradients in the water column. Experiments and field observations demonstrated that all age and size groups of Arctodiaptomus salinus and Brachionus plicatilis strongly avoided surface layers during the daylight. The escape of zooplankton from the anoxic hypolimnion was less active. Statistically significant avoidance was observed only for copepodites C4-C5 and females of A. salinus. The relatively simple construction of the columns and easy handling during the experiment were the factors that favoured the use of this device to perform in situ basic tests of the effect of different factors on the vertical distribution of zooplankton. В© Springer 2005.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, Akademgorodok, 660036, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tolomeyev, A.P.; Zadereev, Y.S.

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10.

Surface bonding states of nano-crystalline diamond balls / J. L. Peng [et al.] // International Journal of Modern Physics B. - 2001. - Vol. 15, Is. 31. - P4071-4085, DOI 10.1142/S0217979201007865 . - ISSN 0217-9792
Кл.слова (ненормированные):
diamond -- article -- crystal structure -- electron -- energy transfer -- nanoparticle -- particulate matter -- structure analysis -- surface property -- transmission electron microscopy
Аннотация: The rough surface of nano-crystalline diamond spheres induces surface electronic states which appear as a broadened pre-peak over approx. 15 eV at the C K-edge energy threshold for carbon in the parallel electron energy loss spectrum (PEELS). This appears to be at least partially due to 1s-?* transitions, although typically the latter occupy a range of only 4 eV for the sp2 edge of highly-oriented pyrollytic graphite (HOPG). No ?* electrons appear in the conduction band inside the diamond particles, where all electrons are sp3 hybridized. PEELS data were also obtained from a chemical vapour deposited diamond film (CVDF) and gem-quality diamond for comparison with the spectra of nano-diamonds. The density of Sp2 and Sp3 states on the surface of diamond nano-crystals is calculated for simple structural models of the diamond balls, including some conjecture about surface structures. The results are used to interpret the sp2/sp3 ratios measured from the PEELS spectra recorded as scans across the particles. Surface roughness at the atomic scale was also examined using high-resolution transmission electron microscopy (HRTEM) and electron nano-diffraction patterns were used to confirm the crystal structures.

Scopus
Держатели документа:
Department of Applied Physics, RMIT University, Swanston Street, Melbourne, Vic. 3051, Australia
Electron Microscope Unit, University of Sydney, NSW 2006, Australia
Molecular Architecture Group, Kirensky Institute of Physics, Institute of Biophysics, 660036 Krasnoyarsk, Russian Federation
School of Physics, University of Melbourne, Parkville, Vic. 3010, Australia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Peng, J.L.; Bulcock, S.; Belobrov, P.I.; Bursill, L.A.

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11.

Ground truth methods as a part of space mapping of inland water phytopigment dynamics / A. P. Shevyrnogov, A. F. Sid'ko // Advances in Space Research. - 1998. - Vol. 22, Is. 5. - P705-708, DOI 10.1016/S0273-1177(97)01137-X . - ISSN 0273-1177
Кл.слова (ненормированные):
chlorophyll -- optical property -- spectral reflectance -- Russian Federation
Аннотация: Spectral radiance and reflectance of the Krasnoyarsk reservoir water surface were studied from 1974 until 1989. Spectral brightness coefficient (SBC) curves have been found to record chlorophyll absorption band in the red part of the spectrum ? = 680 nm and spectral indices of 'yellow matter' absorption in the short-wave part ? = 400-500 nm. Blue-green algae SBC curves record phycocyanine absorption peak at ?max = 630 mm. The peak is inherent in this type of algae only.

Scopus
Держатели документа:
Institue of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shevyrnogov, A.P.; Sid'ko, A.F.

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12.

Feeding spectra of Arctodiaptomus salinus (Calanoida, Copepoda) using fatty acid trophic markers in seston food in two salt lakes in South Siberia (Khakasia, Russia) / A. P. Tolomeev [et al.] // Aquatic Ecology. - 2010. - Vol. 44, Is. 3. - P513-530, DOI 10.1007/s10452-010-9331-y . - ISSN 1386-2588
Кл.слова (ненормированные):
Arctodiaptomus salinus -- Brackish lakes -- Fatty acids -- Feeding spectra -- Salinity adaptation -- adaptation -- bacterium -- brackish water -- ciliate -- comparative study -- crustacean -- cyanobacterium -- fatty acid -- feeding behavior -- flagellate -- food selection -- green alga -- lipid -- physicochemical property -- picoplankton -- saline lake -- salinity -- seasonality -- seston -- Khakassia -- Russian Federation -- Siberia -- Animalia -- Arctodiaptomus salinus -- Bacillariophyta -- Bacteria (microorganisms) -- Calanoida -- Chlorophyta -- Ciliophora -- Copepoda -- Cryptomonas -- Cryptophyta -- Cyanobacteria -- Mastigophora (flagellates) -- Proteobacteria
Аннотация: During two vegetation seasons (2004-2005), we compared feeding spectra of Arctodiaptomus salinus (Calanoida, Copepoda) populations inhabiting two neighboring salt lakes, Shira and Shunet, Khakasia, Russia, using fatty acid (FA) trophic markers. Sestonic FA composition in two lakes moderately differed, whereas levels of diatom FA markers were higher in Lake Shunet and of Cyanobacteria and green algae markers in Lake Shira. In general, markers in storage lipids-triacylglycerols (TAG) of A. salinus-reflected the differences in sestonic composition of the two lakes. Nevertheless, TAG fraction was also enriched by FA trophic markers of the minor components of seston, which were selectively ingested by the animals. In Lake Shira, A. salinus had significantly higher concentrations of bacterial FA markers in TAG. In Lake Shunet, TAG of A. salinus contained significantly higher relative amounts of 18:4?3, 18:5?3 and C22 polyunsaturated fatty acids (PUFA), which indicated marked contribution of cryptophytes or (and) flagellates into the diet. Laboratory experiments showed feeding on Cryptomonas and sulfur purple bacteria in Lake Shunet and ciliates and colonial picoplankton in both lakes, and generally confirmed the differences in FA trophic markers in A. salinus between the lakes. The two populations of A. salinus markedly differed in levels of essential long-chain PUFA, eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, although the levels of these FA in seston were quite similar between the two lakes. The higher levels of the essential ?3 PUFA in A. salinus in Lake Shunet may be an adaptive response of the animals to a vertical stratification of physico-chemical conditions and significantly higher salinity levels at the boundary of adjacent bottom layer in this lake. В© 2010 Springer Science+Business Media B.V.

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Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russian Federation
Siberian Federal University, Svobodny av. 79, 660041 Krasnoyarsk, Russian Federation
Netherlands Institute of Ecology, Department of Aquatic Ecology, Rijksstraatweg 6, 3631 AC Nieuwersluis, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tolomeev, A.P.; Sushchik, N.N.; Gulati, R.D.; Makhutova, O.N.; Kalacheva, G.S.; Zotina, T.A.

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13.

Use of proZZ-obelin fusion protein in bioluminescent immunoassay [Text] / L. A. Frank, V. A. Illarionova, E. S. Vysotski // Biochem. Biophys. Res. Commun. - 1996. - Vol. 219, Is. 2. - P475-479, DOI 10.1006/bbrc.1996.0258. - Cited References: 21 . - 5. - ISSN 0006-291X

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
ESCHERICHIA-COLI
   EXPRESSION
   AEQUORIN
   PURIFICATION
   SYSTEM
Аннотация: Obelin is a photoprotein that emits light by Ca2+-binding. To develop a bioluminescent immunoassay based on the light emission property of obelin, we have expressed the apoobelin fusion protein with ZZ-domain of S. aureus protein A in E. coil by recombinant DNA techniques. The pro2Z-obelin expressed was purified by one-step affinity chromatography on IgG-Agarose. The purified proZZ-obelin has both the luminescent activity of obelin and the IgG-binding ability of ZZ-domain. The specific activity of fusion protein was 8.5 x 10(15) photons per mg of protein. (C) 1996 Academic Press, Inc.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Frank, L.A.; Illarionova, V.A.; Vysotski, E.S.

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14.

Expression, purification and characterization of the secreted luciferase of the copepod Metridia longa from Sf9 insect cells [Text] / G. A. Stepanyuk [et al.] // Protein Expr. Purif. - 2008. - Vol. 61, Is. 2. - P142-148, DOI 10.1016/j.pep.2008.05.013. - Cited References: 34. - This work was supported by the National Institutes of Health (Grant 1P50 GM62407), University of Georgia Research Foundation and Georgia Research Alliance, the Russian Foundation for Basic Research and Taiwan National Science Council (Grant 06-0489502) and the program for "Molecular and Cellular Biology" of Russian Academy of Sciences. . - ISSN 1046-5928

РУБ Biochemical Research Methods + Biochemistry & Molecular Biology + Biotechnology & Applied Microbiology
Рубрики:
VARGULA-HILGENDORFII LUCIFERASE
   CRYSTAL-STRUCTURE
   RENILLA-RENIFORMIS
   GAUSSIA LUCIFERASE
   BIOLUMINESCENT REPORTER
   OBELIN BIOLUMINESCENCE
   ANGSTROM RESOLUTION
   MAMMALIAN-CELLS
   GENE-EXPRESSION
   IN-VIVO
Аннотация: Metridia luciferase is a secreted luciferase from a marine copepod and uses coelenterazine as a substrate to produce a blue bioluminescence This luciferase has been successfully applied as a bioluminescent reporter in mammalian cells. The main advantage of secreted luciferase as a reporter is the capability of measuring intracellular events without destroying the cells or tissues and this property is well suited for development of high throughput screening technologies. However because Metridia luciferase is a Cys-rich protein, Escherichia coli expression systems produce an incorrectly folded protein, hindering its biochemical characterization and application for development of in vitro bioluminescent assays. Here we report the successful expression of Metridia luciferase with its signal peptide for secretion, in insect (Sf9) cells using the baculovirus expression system. Functionally active luciferase secreted by insect cells into the culture media has been efficiently purified with a yield of high purity protein of 2-3mg/L This Metridia luciferase expressed in the insect cell system is a monomeric protein showing 3.5-fold greater bioluminescence activity than luciferase expressed and purified from E. coli. The near coincidence of the experimental mass of Metridia luciferase purified from insect cells with that calculated from amino acid sequence. indicates that luciferase does not undergo post-translational modifications such as phosphorylation or glycosylation and also, the cleavage site of the signal peptide for secretion is at VQA-KS, as predicted from sequence analysis. (c) 2008 Elsevier Inc. All rights reserved.

Держатели документа:
[Stepanyuk, Galina A.
Markova, Svetlana V.
Vysotski, Eugene S.] Russian Acad Sci, Photobiol Lab, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
[Stepanyuk, Galina A.
Xu, Hao
Wu, Chia-Kuei
Lee, John
Vysotski, Eugene S.
Wang, Bi-Cheng] Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Stepanyuk, G.A.; Xu, H...; Wu, C.K.; Markova, S.V.; Lee, J...; Vysotski, E.S.; Wang, B.C.

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15.

Self-restoration as fundamental property of CES providing their sustainability [Text] / I. I. Gitelson, A. G. Degermendzhy, E. K. Rodicheva ; ed. M Nelson [et al.] // SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P. 1641-1648, DOI 10.1016/S0273-1177(03)00111-X. - Cited References: 8 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Ecology + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences
Рубрики:
LIFE-SUPPORT
Аннотация: Sustainability is one of the most important criteria in the creation and evaluation of human life support systems intended for use during long space flights. The common feature of biological and physicochemical life support systems is that basically they are both catalytic. But there are two fundamental properties distinguishing biological systems: 1) they are auto-catalytic: their catalysts - enzymes of protein nature - are continuously reproduced when the system functions; 2) the program of every process performed by enzymes and the program of their reproduction are inherent in the biological system itself - in the totality of genomes of the species involved in the functioning of the ecosystem. Actually, one cell with the genome capable of the phenotypic realization is enough for the self-restoration of the function performed by the cells of this species in the ecosystem. The continuous microalgal culture of Chlorella vulgaris was taken to investigate quantitatively the process of self-restoration in unicellular algae population. Based on the data obtained, we proposed a mathematical model of the restoration process in a cell population that has suffered an acute radiation damage. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

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Держатели документа:
Krasnoyarsk State Univ, Inst Biophys, SB RAS, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, I.I.; Degermendzhy, A.G.; Rodicheva, E.K.; Nelson, M \ed.\; Pechurkin, NS \ed.\; Dempster, WF \ed.\; Somova, LA \ed.\; Somo, , LA \ed.\

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16.

The smallest natural high-active luciferase: Cloning and characterization of novel 16.5-kDa luciferase from copepod Metridia longa [Text] / S. V. Markova [et al.] // Biochem. Biophys. Res. Commun. - 2015. - Vol. 457, Is. 1. - P77-82, DOI 10.1016/j.bbrc.2014.12.082. - Cited References:20. - The cloning of cDNA encoding MLuc7 luciferase of M. longa was supported by Bayer AG (Germany); all other studies - by the grant 14-14-01119 of the Russian Science Foundation. We declare that authors have no conflict of interest. . - ISSN 0006-291X. - ISSN 1090-2104

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
CDNA CLONING
   SECRETED LUCIFERASE
   ESCHERICHIA-COLI
   EXPRESSION
Кл.слова (ненормированные):
Bioluminescence -- Coelenterazine -- Copepod luciferase -- Mammalian -- expression -- Real-time imaging
Аннотация: Coelenterazine-dependent copepod luciferases containing natural signal peptide for secretion are a very convenient analytical tool as they enable monitoring of intracellular events with high sensitivity, without destroying cells or tissues. This property is well suited for application in biomedical research and development of cell-based assays for high throughput screening. We report the cloning of cDNA gene encoding a novel secreted non-allelic 16.5-kDa isoform (MLuc7) of Metridia longa luciferase, which, in fact, is the smallest natural luciferase of known for today. Despite the small size, isoform contains 10 conservative Cys residues suggesting the presence of up to 5 S-S bonds. This hampers the efficient production of functionally active recombinant luciferase in bacterial expression systems. With the use of the baculovirus expression system, we produced substantial amounts of the proper folded MLuc7 luciferase with a yield of similar to 3 mg/L of a high purity protein. We demonstrate that MLuc7 produced in insect cells is highly active and extremely thermostable, and is well suited as a secreted reporter when expressed in mammalian cells ensuring higher sensitivity of detection as compared to another Metridia luciferase isoform (MLuc164) which is widely employed in real-time imaging. (C) 2014 Elsevier Inc. All rights reserved.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Photobiol Lab, Siberian Branch, Krasnoyarsk, Russia.
Siberian Fed Univ, Chair Biophys, Krasnoyarsk, Russia.
ИБФ СО РАН

Доп.точки доступа:
Markova, Svetlana V.; Larionova, Marina D.; Burakova, Ludmila P.; Vysotski, Eugene S.; Bayer AG (Germany); Russian Science Foundation [14-14-01119]

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17.

Physicochemical properties of multicomponent polyhydroxyalkanoates: Novel aspects / T. G. Volova [et al.] // Polym. Sci. Ser. A. - 2017. - Vol. 59, Is. 1. - P98-106, DOI 10.1134/S0965545X17010163 . - ISSN 0965-545X
Кл.слова (ненормированные):
Glycols -- Medical problems -- Melting -- Molecular mass -- Monomers -- Polymers -- Chemical compositions -- Degree of crystallinity -- Degrees of crystallinity -- Multi-component polyhydroxyalkanoates -- Physicochemical property -- Polyhydroxyalkanoates -- Temperature intervals -- Thermal degradation temperatures -- Organic polymers
Аннотация: The physicochemical properties such as the degree of crystallinity and temperature and molecularmass characteristics of a number of polyhydroxyalkanoates of various chemical composition synthesized on a complex carbon substrate by bacteria Cupriavidus eutrophus В10646 have been investigated. Two-, three-, and four-component copolymer samples have different sets and ratios of monomers with various lengths of carbon chains: 3-hydroxybutyrate (3HB), 4-hydroxybutyrate (4HB), 3-hydroxyvalerate (3HV), 3-hydroxyhexanoate (3HH), 3-hydroxy-4-methyl valerate (3H4MV), and diethylene glycol (DEG). It has been shown that weight-average molar mass Мw and polydispersity vary in a wide range with no correlation existing with the composition of copolymer polyhydroxyalkanoates and that thermal stability is preserved in the temperature interval between the melting temperature and the thermal degradation temperature from 100 to 120–140°С. The composition and ratio of monomers most notably affect the degree of crystallinity of polyhydroxyalkanoates. Significant differences between the degrees of crystallinity of three- and four-component polyhydroxyalkanoates have been found for the first time. The degree of crystallinity for copolymers P(3HB/3HV/4HB) is 9–22%, and the degree of crystallinity for copolymers P(3HB/3HV/3HH) and P(3HB/3GV/3H4MV) is 41–63%; this value is close to the degree of crystallinity for diblock copolymers P(3HB)/DEG, which is 56–69%. For the four-component copolymers P(3HB/3GV/4HB/3HH), the degree of crystallinity is 30–41%. The values of Мw for the copolymers P(3HB/DEG) are inhomogeneous and the polymers contain fractions uneven with respect to molecular mass: a high-molecular-mass polymer (Мw from 2700 to 4900 kDa) and a low-molecular-mass polymer (Мw = 46–167 kDa). For the copolymers P(3HB)/DEG and P(3HB/3HV/3H4MV), two peaks are observed in the region of melting with the gap between these peaks being 4–20°С. All of the types of copolymer samples, regardless of the monomer ratio, show an increase in elongation at break against the background of a decrease in tensile stress and Young’s modulus, with these effects being pronounced to different extents. On the whole, the properties of multicomponent polyhydroxyalkanoates differ appreciably. © 2017, Pleiades Publishing, Ltd.

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Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Volova, T. G.; Vinogradova, O. N.; Zhila, N. O.; Kiselev, E. G.; Peterson, I. V.; Vasil’ev, A. D.; Sukovatyi, A. G.; Shishatskaya, E. I.

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18.

Biosynthesis and properties of P(3HB-co-3HV-co-3H4MV) produced by using the wild-type strain Cupriavidus eutrophus B-10646 / T. Volova [et al.] // Journal of Chemical Technology and Biotechnology. - 2018, DOI 10.1002/jctb.5763 . - Article in press. - ISSN 0268-2575
Кл.слова (ненормированные):
3-hydroxy-4-methylvalerate -- physicochemical properties -- polyhydroxyalkanoates -- synthesis -- Biochemistry -- Chemical industry -- Synthesis (chemical) -- 3-hydroxy-4-methylvalerate -- Crystalline-to-amorphous -- Degree of crystallinity -- Degrees of crystallinity -- Physicochemical property -- Polyhydroxyalkanoates -- Processing properties -- Wild-type strain -- Polymers
Аннотация: BACKGROUND: Polyhydroxyalkanoates (PHA) containing 3-hydroxy-4-methylvalerate (3H4MV) have better processing properties than other PHA types and were not prone to ageing. The main challenge in the production of these polymers is to simultaneously achieve high cell biomass, high polymer content, and high molar fraction of 3H4MV. The aim of this study was to investigate the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxy-4-methylvalerate) [P(3HB-co-3 HV-co-3H4MV)] by Cupriavidus eutrophus B-10646, synthesize polymers with different contents of 3H4MV and investigate their properties. RESULTS: It was shown that 4-methylvalerate (4MV, the precursor substrate) concentration in the culture medium must not be higher than 1 g L?1, and that 4MV must be carefully dosed, with at least 10–12 h intervals between supplementations. A series of polymers with molar fractions of 3H4MV between 2.7 and 11.3 mol% was synthesized. Degrees of crystallinity, molecular weight characteristics, and thermal properties of the polymers have been investigated as dependent on proportions of monomers. CONCLUSION: The content of 3H4MV depends on the concentration of 4MV and conditions of carbon nutrition. The molar fraction of 3H4MV had a strong effect on the crystalline to amorphous region ratio. PHA containing 3H4MV specimens synthesized in this study had a degree of crystallinity of below 50%, which remained unchanged for 2.5 years. © 2018 Society of Chemical Industry. © 2018 Society of Chemical Industry

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Держатели документа:
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
L.V. Kirenskii Institute of Physics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
Institute of Chemistry and Chemical Technology SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
International and Inter University Centre of Nanoscience and Nanotechnology, Mahatam Gandhi University, Kottyam, India

Доп.точки доступа:
Volova, T.; Menshikova, O.; Zhila, N.; Vasiliev, A.; Kiselev, E.; Peterson, I.; Shishatskaya, E.; Thomas, S.

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19.

A heuristic neural network model in the research of properties of evolutionary trajectories / S. Bartsev, P. Baturina // IOP Conference Series: Materials Science and Engineering : Institute of Physics Publishing, 2019. - Vol. 537: International Workshop on Advanced Technologies in Material Science, Mechanical and Automation Engineering - MIP: Engineering-2019 (4 April 2019 through 6 April 2019, ) Conference code: 149243, Is. 4, DOI 10.1088/1757-899X/537/4/042001
Кл.слова (ненормированные):
Molecular biology -- Artificial networks -- Common property -- Evolving systems -- Fitness functions -- Heuristic model -- Molecular evolution -- Neural network model -- Trajectory formation -- Heuristic methods
Аннотация: There is considerable data on molecular evolution, but there remains no approach to systematizing them within the framework of the key problems of biology. To search for the most common properties of evolving systems, the heuristic method has been proposed. Artificial networks of formal neurons were chosen as the heuristic model object. The paper examines the divergent component of evolutionary trajectory formation. As a result of the simulation, the dependence of the potential variability parameter on the position of the fitness function landscape was obtained. The simulation results are in agreement with the real data of molecular evolution experiments. © Published under licence by IOP Publishing Ltd.

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Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center, Krasnoyarsk Scientific Center SB RAS, 50, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, 79 Svobodny pr., Krasnoyarsk, 660041, Russian Federation

Доп.точки доступа:
Bartsev, S.; Baturina, P.

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20.

Transformation of soil and land resources of the Middle Siberia in the conditions of climatic changes / A. A. Shpedt, N. A. Ligaeva, D. V. Emelyanov // IOP Conference Series: Earth and Environmental Science : Institute of Physics Publishing, 2019. - Vol. 315: International Scientific Conference on Agribusiness, Environmental Engineering and Biotechnologies, AGRITECH 2019 (20 June 2019 through 22 June 2019, ) Conference code: 152072, Is. 5. - Ст. 052051, DOI 10.1088/1755-1315/315/5/052051
Кл.слова (ненормированные):
Biotechnology -- Cultivation -- Environmental technology -- Natural resources -- Annual mean -- Climatic changes -- Land resources -- Linear trends -- SIBERIA -- Soil cover -- Soil property -- Soils
Аннотация: The paper describes the fields of transformation of soil and land resources of the Middle Siberia as the result of long-term climatic changes. The description of soil and land resources is given. The climatic changes have been evaluated for the period from 1919 to 2018, and increase of the annual mean air temperature and amount of precipitation in natural zones of the region has been established on the basis of the analysis of linear trends. Under the impact of these factors, shifting of zone borders occurs, which leads to replacement of the soil cover structure at the species, generic and subtype levels. Changing regimes and soil properties cause the necessity to use new sorts and adapted technologies for crop growing. © 2019 IOP Publishing Ltd. All rights reserved.

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Держатели документа:
Krasnoyarsk Research Institute of Agriculture SB RAS, Federal Research Center Krasnoyarsk Science Center SB RAS, 66 Svobodny Av, Krasnoyarsk, 660037, Russian Federation
Siberian Federal University, 79 Svobodny avenue, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center Krasnoyarsk Science Center SB RAS, Krasnoyarsk 660036, Akademgorodok, Russian Federation

Доп.точки доступа:
Shpedt, A. A.; Ligaeva, N. A.; Emelyanov, D. V.

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