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Large-scale, high-throughput validation of short hairpin RNA sequences for RNA interference [Text] / L. H. Lamarcq [et al.]> // J. Biomol. Screen. - 2006. - Vol. 11, Is. 3. - P236-246, DOI 10.1177/1087057105284342. - Cited References: 50
. - ISSN 1087-0571РУБ Biochemical Research Methods + Biotechnology & Applied Microbiology + Chemistry, Analytical Рубрики: ENZYME FRAGMENT COMPLEMENTATION ORFEOME VERSION 1.1 SMALL NUCLEAR-RNA MAMMALIAN-CELLS GENE-EXPRESSION SIRNA SEQUENCES POLYMERASE-III SELECTION TRANSCRIPTION MICROARRAYS Кл.слова (ненормированные): RNAi -- high-throughput screening -- target validation -- shRNA -- reporter assay Аннотация: (shRNAs) is described. Using this approach, 464 shRNAs auainst 116 different genes were screened for knockdown efficacy, enabling rapid identification of effective shRNAS against 74 genes. Statistical analysis of the effects of various criteria on the activity of the shRNAs confirmed that some of the rules thought to govern small interfering RNA (siRNA) activity also apply to shRNAs. These include moderate GC content, absence of internal hairpins, and asymmetric thermal stability. However, the authors did not find strong support for position-specific rules. In addition, analysis of the data suggests that not all genes are equally susceptible to RNA interference (RNAi).
Держатели документа: Clontech Labs, Mountain View, CA 94043 USA BD Biosci Pharmingen, La Jolla, CA USA Bayer Healthcare AG, Pharma Res, Mol Screening Technol, Wuppertal, Germany Russian Acad Sci, Inst Biophys, Photobiol Lab, Siberian Branch, Krasnoyarsk, Russia ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50 Доп.точки доступа: Lamarcq, L.H.; Scherer, B.J.; Phelan, M.L.; Kalnine, N.N.; Nguyen, Y.H.; Kabakova, T...; Chen, X.Y.; Tan, M...; Chang, C...; Berlon, C...; Campos-Gonzalez, R...; Gao, G.J.; Golz, S...; Vysotski, E.S.; Farmer, A.A. Найти похожие
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