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Физиолого-биохимические своства и способность к синтезу полиоксиалканоатов у глюкозоусваивающего штамма водородных бактерий Ralstonia eutropha B8562 [Текст] : научное издание / Т. Г. Волова [и др.] // Микробиология. - 2005. - Т. 74, N 6. - С. 788-794 . - ISSN 0026-3656

ГРНТИ

34.27.17

Рубрики:
ПОЛИОКСИАЛКАНОАТЫ
   БИОСИНТЕЗ
   RALSTONIA EUTROPHA (BACT.)
   РОДИТЕЛЬСКИЙ ШТАММ B5786
   МУТАНТНЫЙ ШТАММ B8562
   СРАВНЕНИЕ СВОЙСТВ
Аннотация: Исследованы физиолого-биохимические, генетические и культуральные св-ва глюкозоусваивающего мутантного штамма Ralstonia eutropha B8562 в сопоставлении с родительским штаммом R. eutropha B5786. Морфолого-культуральные и биохимические хар-ки штамма R. eutropha B8562 оказались близкими штамму R. eutropha B5786. При выявлении степени генетического родства штаммов обнаружены отличия в последовательности генов 16S рРНК. Показано, что ростовые хар-ки штамма на глюкозе в кач-ве единственного источника углерода и энергии сопоставимы с показателями родительского штамма на фруктозе. Штамм B8562 характеризуется высокими выходами полиоксиалканоатов (ПОА) на различных источниках углерода (CO[2], фруктозе, глюкозе). Накопление ПОА у штамма на глюкозе в периодической культуре при лимитировании роста по азоту достигает 90% от сухого веса. Доминирующим мономером в ПОА является 'бета'-оксимасляная к-та (свыше 99 мол.%), в кач-ве минорных включений идентифицированы 'бета'-оксивалериановая (0,25-0,72 мол.%) и 'бета'-оксигексановая (0,08-1,5 мол.%) к-ты. Штамм перспективен в кач-ве продуцента ПОА на доступных сахаросодержащих средах с глюкозой. Россия, Ин-т биофизики СО РАН, Красноярск. Библ. 20

Держатели документа:
Институт биофизики СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Волова, Т.Г.; Кожевников, И.В.; Долгополова, Ю.Б.; Трусова, М.Ю.; Калачева, Г.С.; Арефьева, Ю.В.

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574.4
В 67

Волова, Т. Г.
Синтез сополимеров 3-гидроксибутирата-со-4гидроксибутирата водородокисляющими бактериями [Текст] : сборник / Т. Г. Волова , Н. 0. Жила [и др.] // Прикладная биохимия и микробиология. - 2011. - Т.47, №5. - с. 544 -550. - Библиогр. в конце ст.

ГРНТИ	34.57.21
УДК	574.4

Аннотация: Исследован синтез сополимера 3- и 4-гидроксибутирата (3ГБ-CO-4ГБ) как наиболее перспективного представителя семейства биоразрушаемых полигидроксиалканоатов (ПГА). С использованием природных штаммов водородокисляющих бактерий Ralstonia eutropha B5786 и Cupriavidus eutrophus В10646 найдены условия культивирования для эффективного синтеза сополимера 3ГБ-CO-4ГБ. Получена серия высокоочищенных образцов сополимера 3ГБ-CO-4ГБ с различным содержанием 4ГБ (от 8.7 до 24.3 мол. %). Установлено, что включение 4ГБ в сополимер в большей степени, нежели 3-гидроксивалерат и 3-гидроксигексаноат, приводит к снижению кристалличности сополимера; получены образцы, имеющие степень кристалличности ниже 30%. Показано, что средневесовая молекулярная масса сополимеров 3ГБ-CO-4ГБ не зависит от соотношения мономеров и варьирует в широких пределах (от 540 до 1110 кДа).

Держатели документа:
Институт биофизики СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Жила, Н. 0.; Калачёва, Г. С.; Соколенко, В. А.; Сински, Э. Дж.

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Волова, Т. Г.
ХАРАКТЕРИСТИКА БЕЛКОВ, СИНТЕЗИРУЕМЫХ ВОДОРОДОКИСЛЯЮЩИМИ МИКРООРГАНИЗМАМИ [Текст] : научное издание / Т. Г. Волова // Прикл. биохимия и микробиол. - 2010. - Т. 46, N 6. - С. 624-629 . - ISSN 0555-1099

ГРНТИ

34.27.39

Рубрики:
БЕЛОК ОДНОКЛЕТОЧНЫХ
   ПОТЕНЦИАЛЬНЫЕ ИСТОЧНИКИ
   ВОДОРОДОКИСЛЯЮЩИЕ МИКРООРГАНИЗМЫ
   ALCALIGENES EUTROPHUS (BACT.)
   ШТАММ Z1
   RALSTONIA EUTROPHA (BACT.)
   ШТАММ B5786
   SILEBERIA CARBOXYDOHYDROGENA (BACT.)
   ШТАММ Z1062
Аннотация: Исследованы белки, синтезируемые водородокисляющими микроорганизмами - водородными бактериями Alcaligenes eutrophus Z1 и Ralstonia eutropha В5786, СО-резистентным штаммом карбоксидобактерий Seliberia carboxydohydrogena Z1062. Показано, что белки водородокисляющих микроорганизмов по ряду основных показателей, характеризующих биологическую ценность, занимают промежуточное положение между традиционными белками животного и растительного происхождения. Высокое общее содержание белка в биомассе, полноценный аминокислотный состав и доступность воздействию протеолитическими ферментами позволяют рассматривать водородокисляющие микроорганизмы в качестве потенциального источника белка

Держатели документа:
Институт биофизики СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

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^a311.25.15.17^2VINITI
Ф 50

Физико-химические свойства одно-[поли(3ГБ)], двух-[поли(3ГБ/3ГВ)] и трехкомпонентных [поли(3ГБ/3ГВ/3ГГ)] полигидроксиалканоатов [Текст] : научное издание / Т. Г. Волова [и др.] // Перспект. матер. - 2004. - N 3. - С. 42-48 . - ISSN 1028-978X

ГРНТИ

31.25.15

РУБ 311.25.15.17
Рубрики:
ПОЛИМЕРЫ
   СОПОЛИМЕРЫ
   ГИДРОКСИАЛКАНОАТЫ
   ФИЗИКО-ХИМИЧЕСКИЕ СВОЙСТВА
Аннотация: С использованием бактерий Ralstonia eutropha B5786 синтезировано семейство полигидроксиалканоатов (ПГА) различного состава: 1-, 2- и 3-х компонентные полимеры, состоящие из мономеров гидроксибутирата (3ГБ), гидроксивалерата (3ГВ) и гидроксигексаноата (3ГГ). С применением рентгеноструктурного анализа, ИК-спектроскопии, ДТА и вискозиметрии исследованы свойства 3-х [поли(3ГБ/3ГВ)] и 2-х [поли(3ГБ/3ГВ/3ГГ)] компонентных полимеров в сопоставлении с гомополимером гидроксимасляной кислоты [поли(3ГБ)]. С ростом молярной фракции 3ГВ отмечено выравнивание соотношения кристаллич. и аморфной фаз в сополимере. Это сопровождалось снижением степени кристалличности материала от 70-80 до 45-50%, особенно наглядно при увеличении молярной фракции 3ГВ от единиц до 25-30 мол.%. Температурные свойства ПГА, температура плавления (T[пл]) и разложения (Т[p]), у сополимеров поли(3ГБ/3ГВ) ниже по сравнению с полигидроксибутиратом, для которого Т[пл] и Т[р] составляют, соответственно, 168-170 и 260-265'ГРАДУС'C. У сополимера с ростом молярной фракции 3ГВ оба параметра снижаются, в изученном диапазоне соотношения мономеров - до 150-160 и 200-220'ГРАДУС'C, соответственно. Влияния включения гидроксигексаноата в ПГА в достигнутых величинах (от 2 до 4 мол.%) на физико-химич. свойства материала не обнаружено. Четкой связи между составом ПГА и величиной молекулярной массы не выявлено

Держатели документа:
Институт биофизики СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Волова, Т.Г.; Плотников, В.Ф.; Шишацкая, Е.И.; Миронов, П.В.; Васильев, А.Д.

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Effects of intracellular poly(3-hydroxybutyrate) reserves on physiological-biochemical properties and growth of Ralstonia eutropha / T. G. Volova [et al.] // Research in Microbiology. - 2013. - Vol. 164, Is. 2. - P164-171, DOI 10.1016/j.resmic.2012.10.008 . - ISSN 0923-2508
Кл.слова (ненормированные):
Granules -- PHA cycle -- Poly(3-hydroxybutyrate) -- Ralstonia eutropha -- poly(3 hydroxybutyric acid) -- article -- bacterial growth -- bacterial strain -- bacterium culture -- cell division -- cell granule -- cell size -- controlled study -- Cupriavidus necator -- electron microscopy -- nonhuman -- polymer production -- priority journal -- Cupriavidus necator -- Cytoplasmic Granules -- Hydroxybutyrates -- Microscopy, Electron, Transmission -- Polyesters -- Bacteria (microorganisms) -- Cupriavidus necator
Аннотация: Microbial polyhydroxyalkanoates (PHAs), because of their well studied complex physiology and commercial potential, are vehicles for carbon and potential storage reduction for many microbial species. Even with the wealth of studies about microbial PHAs in the scientific literature, polymer accumulation and degradation are still not comprehensively understood. Poly(3-hydroxybutyrate) (P3HB) granule formation and polymer mobility were studied here in the bacterium Ralstonia eutropha strain B5786 in autotrophic cultures. Electron microscopy studies revealed decreasing cell size concomitant with enlargement of size and number of intracellular granules, and inhibition of cell division during intracellular polymer production. Activities of key P3HB biosynthetic enzymes demonstrated correlations with each other during polymer accumulation, suggesting an intricately regulated P3HB cycle in autotrophically grown R. eutropha cells. В© 2012 .

Scopus
Держатели документа:
Laboratory of Chemoautotrophic Biosynthesis, Institute of Biophysics of the Siberian Branch of The Russian Academy of Sciences, Akademgorodok 50, Krasnoyarsk 660036, Russian Federation
Department of Biotechnology, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Avenue, Krasnoyarsk 660041, Russian Federation
Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge 02139, MA, United States
Health Sciences Technology Division, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge 02139, MA, United States
Engineering Systems Division, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge 02139, MA, United States : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Zhila, N.O.; Kalacheva, G.S.; Brigham, C.J.; Sinskey, A.J.

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Synthesis of 3-hydroxybutyrate-CO-4-hydroxybutyrate copolymers by hydrogen-oxidizing bacteria / T. G. Volova [et al.] // Applied Biochemistry and Microbiology. - 2011. - Vol. 47, Is. 5. - P494-499, DOI 10.1134/S0003683811050152 . - ISSN 0003-6838
Кл.слова (ненормированные):
Bacteria (microorganisms) -- Cupriavidus -- Cupriavidus necator
Аннотация: Synthesis of 3- and 4-hydroxybutyrate copolymer (3HB-co-4HB), the most promising member of the biodegradable polyhydroxyalkanoate (PHA) family, has been studied. Cultivation conditions of naturally occurring strains of hydrogen-oxidizing bacteria Ralstonia eutropha B5786 and Cupriavidus eutrophus B10646 have been optimized to ensure efficient synthesis of the 3HB-co-4HB copolymer. A set of highly pure samples of the 3HB-co-4HB copolymer with 4HB content varying from 8.7 to 24.3 mol% has been obtained. Incorporation of 4HB into the copolymer was shown to cause a more pronounced decrease in polymer crystallinity than the incorporation of 3-hydroxyvalerate or 3-hydroxyhexanoate; samples with a degree of crystallinity below 30% have been obtained. The weight average molecular mass of the 3HB-co-4HB copolymers was shown to be independent on the monomer ratio and to vary broadly (from 540 to 1110 kDa). В© 2010 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Siberian Federal University, Krasnoyarsk 660041, Russian Federation
Biophysics Institute, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation
Chemistry and Chemical Technology Institute, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation
Massachusetts Institute of Technology, Cambridge, MA 02139, United States : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Zhila, N.O.; Kalacheva, G.S.; Sokolenko, V.A.; Sinski, E.J.

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Characteristics of proteins synthesized by hydrogen-oxidizing microorganisms / T. G. Volova, V. A. Barashkov // Applied Biochemistry and Microbiology. - 2010. - Vol. 46, Is. 6. - P574-579, DOI 10.1134/S0003683810060037 . - ISSN 0003-6838
Кл.слова (ненормированные):
Animalia -- Bacteria (microorganisms) -- Cupriavidus necator -- Pseudomonas carboxydohydrogena
Аннотация: The study was conducted to determine the biological value of proteins synthesized by hydrogen-oxidizing microorganisms-the hydrogen bacteria Alcaligenes eutrophus Z1 and Ralstonia eutropha B5786 and the CO-resistant strain of carboxydobacterium Seliberia carboxydohydrogena Z1062. Based on a number of significant parameters characterizing the biological value of a product, the proteins of hydrogen-oxidizing microorganisms have been found to occupy an intermediate position between traditional animal and plant proteins. The high total protein in biomass of these microorganisms, their complete amino acid content, and availability to proteolytic enzymes allow for us to consider these microorganisms as potential protein producers. В© 2010 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Barashkov, V.A.

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Cloning and molecular organization of the polyhydroxyalkanoic acid synthase gene (phaC) of Ralstonia eutropha strain B5786 / I. V. Kozhevnikov [et al.] // Applied Biochemistry and Microbiology. - 2010. - Vol. 46, Is. 2. - P140-147, DOI 10.1134/S0003683810020031 . - ISSN 0003-6838
Кл.слова (ненормированные):
Aeromonas punctata -- Cupriavidus necator -- Ectothiorhodospira shaposhnikovii -- Pseudomonas -- Pseudomonas sp. 61-3 -- Rhodococcus -- Rhodococcus ruber -- Rhodospirillum rubrum -- Thiococcus pfennigii
Аннотация: Class I polyhydroxyalkanoic acid (PHA) synthase gene (phaC) of Ralstonia eutropha strain B5786 was cloned and characterized. R. eutropha B5786 features the ability to synthesize multicomponent PHAs with short- and medium-chain-length monomers from simple carbohydrate substrate. A correlation was made between the molecular structure of PHA synthase and substrate specificity and the ability of strain-producers to accumulate PHAs of this or that structure. A strong similarity of PHA synthase of R. eutropha strain B5786 with PHA synthase of R. eutropha strain H16, which, as opposed to strain B5786, enables to incorporate medium chain length PHAs if hexanoate is used as carbon source, exhibited 99%. A correlation between the structure of PHA synthase of B5786 strain with synthases of microorganisms which synthesize short and medium chain length PHAs similarly to B5786 strain, showed an identity level from 26 to 41% (homology with synthase of Rhodospirillum rubrum makes 41%, Ectothiorhodospira shaposhnikovii makes 26%, Aeromonas punctata makes 40%, Thiococcus pfennigii makes 28%, Rhodococcus ruber makes 38%, and with PhaCl and PhaC2 synthases of Pseudomonas sp. 61-3 makes 34 and 37%, respectively). This allows for speaking about the absence of a direct connection between the molecular organization of PHA synthases and their functional abilities, namely, the ability to synthesize PHAs of a particular composition. В© 2010 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Siberian Federal University, Krasnoyarsk 660041, Russian Federation
Institute of Biophysics of Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation
Institute of Molecular Microbiology and Biotechnology, B-48149 Munster, Germany : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kozhevnikov, I.V.; Volova, T.G.; Hai, T.; Steinbuchel, A.

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Physiological-biochemical properties and the ability to synthesize polyhydroxyalkanoates of the glucose-utilizing strain of the hydrogen bacterium Ralstonia eutropha B8562 / T. G. Volova [et al.] // Applied Microbiology and Biotechnology. - 2006. - Vol. 73, Is. 3. - P429-433, DOI 10.1007/s00253-006-0460-0 . - ISSN 0175-7598
Кл.слова (ненормированные):
Biochemistry -- Carbon -- Genetic engineering -- Glucose -- Hydrogen -- Polyesters -- Cultural properties -- Glucose-utilizing mutant strain -- Nitrogen deficiency -- Polyhydroxyalkanoate (PHA) -- Bacteria -- 3 hydroxybutyric acid -- carbon dioxide -- fructose -- glucose -- hydrogen -- monomer -- polyhydroxyalkanoic acid -- sugar -- bacterium -- carbon -- comparative study -- energy -- ester -- experimental study -- glucose -- hydrogen -- physicochemical property -- article -- bacterial strain -- bacterium culture -- bacterium mutant -- biomass -- chemical analysis -- Cupriavidus necator -- energy consumption -- genetic analysis -- nonhuman -- synthesis -- Bacteria (microorganisms) -- Cupriavidus necator
Аннотация: Physiological-biochemical, genetic, and cultural properties of the glucose-utilizing mutant strain Ralstonia eutropha B8562 have been compared with those of its parent strain R. eutropha B5786. It has been shown that growth characteristics of the strain cultured on glucose as the sole carbon and energy source are comparable with those of the parent strain. Strain B8562 is characterized by high polyhydroxyalkanoate (PHA) yields on different carbon sources (CO2, fructose, and glucose). PHA accumulation in the strain batch cultured on glucose under nitrogen deficiency reaches 90 %. The major monomer in the PHA is ?-hydroxybutyric acid (more than 99 mol %); the identified minor components are ?-hydroxyvaleric acid (0.25-0.72 mol %) and ?-hydroxyhexanoic acid (0.08-1.5 mol %). The strain is a promising PHA producer on available sugar-containing media with glucose. В© Springer-Verlag 2006.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russian Federation
Krasnoyarsk State University, Svobodny av. 79, 660041 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Trusova, M.Y.; Kalacheva, G.S.; Kozhevnicov, I.V.

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10.

Physiological-biochemical properties and the ability to synthesize polyhydroxyalkanoates of the glucose-utilizing strain of the hydrogen bacterium Ralstonia eutropha B8562. / T. G. Volova [et al.] // Applied microbiology and biotechnology. - 2006. - Vol. 73, Is. 2. - P429-433, DOI 10.1007/s00253-006-0460-0 . - ISSN 0175-7598
Кл.слова (ненормированные):
beta hydroxyvaleric acid -- beta-hydroxyvaleric acid -- carbon dioxide -- ester -- fatty acid -- fructose -- glucose -- hexanoic acid derivative -- hydrogen -- hydroxyacid -- oxygen -- polymer -- ribosome RNA -- valeric acid derivative -- article -- biotechnology -- chemistry -- metabolism -- methodology -- Wautersia eutropha -- Biotechnology -- Carbon Dioxide -- Cupriavidus necator -- Esters -- Fatty Acids -- Fructose -- Glucose -- Hexanoic Acids -- Hydrogen -- Hydroxy Acids -- Oxygen -- Pentanoic Acids -- Polymers -- RNA, Ribosomal
Аннотация: Physiological-biochemical, genetic, and cultural properties of the glucose-utilizing mutant strain Ralstonia eutropha B8562 have been compared with those of its parent strain R. eutropha B5786. It has been shown that growth characteristics of the strain cultured on glucose as the sole carbon and energy source are comparable with those of the parent strain. Strain B8562 is characterized by high polyhydroxyalkanoate (PHA) yields on different carbon sources (CO(2), fructose, and glucose). PHA accumulation in the strain batch cultured on glucose under nitrogen deficiency reaches 90 %. The major monomer in the PHA is beta-hydroxybutyric acid (more than 99 mol %); the identified minor components are beta-hydroxyvaleric acid (0.25-0.72 mol %) and beta-hydroxyhexanoic acid (0.08-1.5 mol %). The strain is a promising PHA producer on available sugar-containing media with glucose.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Trusova, M.Y.; Kalacheva, G.S.; Kozhevnicov, I.V.

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11.

The synthesis of hydroxybutyrate and hydroxyvalerate copolymers by the bacterium Ralstonia eutropha / T. G. Volova, G. S. Kalacheva // Mikrobiologiya. - 2005. - Vol. 74, Is. 1. - С. 63-69 . - ISSN 0026-3656
Кл.слова (ненормированные):
?-ketothiolase -- Controlled synthesis -- Poly(hydroxybutyrate-co-hydroxyvalerate) -- Ralstonia eutropha -- Bacteria (microorganisms) -- Cupriavidus necator -- acetoacetyl coenzyme a -- acetyl coenzyme A acyltransferase -- acyl coenzyme A -- acyltransferase -- butyric acid derivative -- carbon dioxide -- fructose -- hydrogen -- poly(3 hydroxybutyrate) co (3 hydroxyvalerate) -- poly(3-hydroxyalkanoic acid) synthase -- poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate) -- polyester -- polyhydroxyalkanoate synthase -- valeric acid derivative -- article -- chemistry -- crystallization -- culture medium -- metabolism -- Wautersia eutropha -- Acetyl-CoA C-Acyltransferase -- Acyl Coenzyme A -- Acyltransferases -- Butyrates -- Carbon Dioxide -- Crystallization -- Culture Media -- Cupriavidus necator -- Fructose -- Hydrogen -- Polyesters -- Valerates
Аннотация: The paper deals with the study of the synthesis of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) copolymers by the bacterium Ralstonia eutropha B-5786 grown under different carbon nutrition conditions (growth on carbon dioxide, fructose, and CO2-valerate and fructose-valerate mixtures). The parameters to be analyzed included the yield of biomass, the yield, synthesis rate, and composition of copolymers, the activity of the key enzymes of polyhydroxyalkanoate (PHA) synthesis (?-ketothiolase, acetoacetyl-CoA reductase, and PHA synthase), the maximum tolerable concentration of valerate to the bacterium, and the conditions that govern the incorporation of hydroxyvalerate to copolymers. This allowed the relationship between cultivation conditions and the proportion of monomers in the copolymers to be deduced. We were able to synthesize a range of 3HB/3HV copolymers and found that the thermal characteristics and the degree of crystallinity of these copolymers depend on the molar fraction of 3HV.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.

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12.

Physiological and biochemical characteristics and capacity for polyhydroxyalkanoates synthesis in a glucose-utilizing strain of hydrogen-oxidizing bacteria, Ralstonia eutropha B8562 / T. G. Volova [и др.] // Mikrobiologiya. - 2005. - Vol. 74, Is. 6. - С. 788-794 . - ISSN 0026-3656
Кл.слова (ненормированные):
Glucose-utilizing strain -- Hydrogen-oxidizing bacteria -- Polyhydroxyalkanoates -- Synthesis -- Bacteria (microorganisms) -- Cupriavidus necator -- bacterial RNA -- glucose -- hydrogen -- hydroxybutyric acid -- polymer -- RNA 16S -- article -- culture medium -- genetics -- growth, development and aging -- metabolism -- mutation -- oxidation reduction reaction -- physiology -- Wautersia eutropha -- Culture Media -- Cupriavidus necator -- Glucose -- Hydrogen -- Hydroxybutyrates -- Mutation -- Oxidation-Reduction -- Polymers -- RNA, Bacterial -- RNA, Ribosomal, 16S
Аннотация: The physiological, biochemical, genetic, and cultural characteristics of the glucose-utilizing mutant strain Ralstonia eutropha B8562 were investigated in comparison with the parent strain R. eutropha B5786. The morphological, cultural, and biochemical characteristics of strain R. eutropha B8562 were similar to those of strain R. eutropha B5786. Genetic analysis revealed differences between the 16S rRNA gene sequences of these strains. The growth characteristics of the mutant using glucose as the sole carbon and energy source were comparable with those of the parent strain grown on fructose. Strain B8562 was characterized by high yields of polyhydroxyalkanoate (PHA) from different carbon sources (CO 2, fructose, and glucose). In batch culture with glucose under nitrogen limitation, PHA accumulation reached 90% of dry weight. In PHA, ?-hydroxybutyrate was predominant (over 99 mol %); ?-hydroxyvalerate (0.25-0.72 mol %) and ?-hydroxyhexanoate (0.008-1.5 mol %) were present as minor components. The strain has prospects as a PHA producer on glucose-containing media.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Krasnoyarsk State University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kozhevnikov, I.V.; Dolgopolova, Yu.B.; Trusova, M.Yu.; Kalacheva, G.S.; Aref'eva, Yu.V.

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13.

Physiological and biochemical characteristics and capacity for polyhydroxyalkanoates synthesis in a glucose-utilizing strain of hydrogen-oxidizing bacteria, Ralstonia eutropha B8562 / T. G. Volova [et al.] // Microbiology. - 2005. - Vol. 74, Is. 6. - P684-689, DOI 10.1007/s11021-005-0124-6 . - ISSN 0026-2617
Кл.слова (ненормированные):
Glucose-utilizing strain -- Hydrogen-oxidizing bacteria -- Polyhydroxyalkanoates -- Synthesis -- Bacteria (microorganisms) -- Cupriavidus necator
Аннотация: The physiological, biochemical, genetic, and cultural characteristics of the glucose-utilizing mutant strain Ralstonia eutropha B8562 were investigated in comparison with the parent strain R. eutropha B5786. The morphological, cultural, and biochemical characteristics of strain R. eutropha B8562 were similar to those of strain R. eutropha B5786. Genetic analysis revealed differences between the 16S rRNA gene sequences of these strains. The growth characteristics of the mutant using glucose as the sole carbon and energy source were comparable with those of the parent strain grown on fructose. Strain B8562 was characterized by high yields of polyhydroxyalkanoate (PHA) from different carbon sources (CO2, fructose, and glucose). In batch culture with glucose under nitrogen limitation, PHA accumulation reached 90% of dry weight. In PHA, ?-hydroxybutyrate was predominant (over 99 mol %); ?-hydroxyvalerate (0.25-0.72 mol %) and ?-hydroxyhexanoate (0.008-1.5 mol %) were present as minor components. The strain has prospects as a PHA producer on glucose-containing media. В© 2005 Pleiades Publishing, Inc.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Krasnoyarsk State University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kozhevnikov, I.V.; Dolgopolova, Yu.B.; Trusova, M.Yu.; Kalacheva, G.S.; Aref'eva, Yu.V.

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14.

The synthesis of hydroxybutyrate and hydroxyvalerate copolymers by the bacterium Ralstonia eutropha / T. G. Volova, G. S. Kalacheva // Microbiology. - 2005. - Vol. 74, Is. 1. - P54-59, DOI 10.1007/s11021-005-0028-5 . - ISSN 0026-2617
Кл.слова (ненормированные):
?-ketothiolase -- Controlled synthesis -- Poly(hydroxybutyrate-co-hydroxyvalerate) -- Ralstonia eutropha -- Bacteria (microorganisms) -- Ralstonia -- Wautersia eutropha
Аннотация: The paper deals with the study of the synthesis of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) copolymers by the bacterium Ralstonia eutropha B-5786 grown under different carbon nutrition conditions (growth on carbon dioxide, fructose, and CO2-valerate and fructose-valerate mixtures). The parameters to be analyzed included the yield of biomass; the yield, synthesis rate, and composition of copolymers; the activity of the key enzymes of polyhydroxyalkanoate (PHA) synthesis ?-ketothiolase, acetoacetyl-CoA reductase, and PHA synthase); the maximum tolerable concentration of valerate to the bacterium; and the conditions that govern the incorporation of hydroxyvalerate into copolymers. This allowed the relationship between cultivation conditions and the proportion of monomers in the copolymers to be deduced. We were able to synthesize a range of 3HB/3HV copolymers and found that the thermal characteristics and the degree of crystallinity of such copolymers depend on the molar fraction of 3HV. В© 2005 Pleiades Publishing, Inc.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.

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15.

Physicochemical properties of two-component polyhydroxyalkanoates, 3-hydroxybutyrate-3-hydroxyvalerate copolymers / T. G. Volova [et al.] // Biophysics. - 2004. - Vol. 49, Is. 6. - P934-942 . - ISSN 0006-3509
Кл.слова (ненормированные):
Hydroxybutyrate-hydroxyvalerate copolymers -- Physicochemical properties -- Polyhydroxyalkanoates -- Structure -- Bacteria (microorganisms) -- Cupriavidus necator
Аннотация: A series of two-component polyhydroxyalkanoates composed of hydroxybutyrate-hydroxyvalerate copolymers with different monomer ratio was obtained with the use of bacteria Ralstonia eutropha B5786. The properties of the polyhydroxyalkanoates in comparison with the homopolymer of hydroxybutyric acid were studied by X-ray diffraction analysis, IR spectroscopy, differential thermal analysis, and viscometry. The ratio of crystalline to amorphous phase in the copolymers tends to unity with increasing hydroxyvalerate content. This is accompanied by a decrease in the degree of crystallinity of the copolymers from 70-80 to 45-50%, the dependence is virtually linear within the range, of hydroxyvalerate mole fraction from several to 25-30 mol%. Thermal characteristics, melting temperature (Tm) and decomposition temperature (Td), of the polyhydroxyalkanoate copolymers are lower than those for polyhydroxybutyrate, whose Tm and Td are 168-170 and 260-265В°C, respectively. Both parameters decrease to 150-160 and 200-220В°C, respectively, when the hydroxyvalerate mole fraction is raised. No distinct correlation between polymer composition and molecular weight has been revealed. Copyright В© 2004 by MAIK "Nauka/Interperiodica".

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Siberian State Technological University, Krasnoyarsk, 660049, Russian Federation
Kirenskii Institute of Physics, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Plotnikov, V.F.; Shishatskaya, E.I.; Mironov, P.V.; Vasil'ev, A.D.

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16.

Study of Ralstonia eutropha culture producing polyhydroxyalkanoates on products of coal processing / T. G. Volova, N. A. Voinov // Prikladnaia biokhimiia i mikrobiologiia. - 2004. - Vol. 40, Is. 3. - С. 296-300 . - ISSN 0555-1099
Кл.слова (ненормированные):
carbon monoxide -- coal -- polyester -- antibiotic resistance -- article -- culture medium -- enzyme specificity -- gas -- growth, development and aging -- metabolism -- microbiology -- Wautersia eutropha -- Carbon Monoxide -- Coal -- Culture Media -- Cupriavidus necator -- Drug Resistance, Microbial -- Gases -- Polyesters -- Substrate Specificity
Аннотация: Kinetic indices of growth, polyhydroxyalkanoate (PHA) accumulation, and gas exchange have been studied in a culture of the carbon monoxide-resistant hydrogen strain Ralstonia eutropha B-5786 grown on a gaseous substrate (GS) obtained by lignite gasification. The GS was shown to be suitable for PHA production. To increase the degree of GS consumption, various modes of gas supply to the culture were tested. Based on the results, an algorithm was developed for calculating and controlling gas-exchange parameters in the PHA-accumulating culture of Ralstonia eutropha, grown on a new GS allowing high polymer yields (up to 75%) and degrees of the substrate utilization (up to 90%).

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division of the Russian Academy of Sciences, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Voinov, N.A.

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17.

Study of a Ralstonia eutropha culture producing polyhydroxyalkanoates on products of coal processing / T. G. Volova, N. A. Voinov // Applied Biochemistry and Microbiology. - 2004. - Vol. 40, Is. 3. - P249-252, DOI 10.1023/B:ABIM.0000025946.47013.03 . - ISSN 0003-6838
Кл.слова (ненормированные):
alkanoic acid -- carbon monoxide -- coal -- hydrogen -- lignite -- algorithm -- article -- bacterial strain -- bacterium culture -- coal gasification -- controlled study -- gas -- gas exchange -- mathematical computing -- nonhuman -- Ralstonia eutropha -- Bacteria (microorganisms) -- Ralstonia -- Wautersia eutropha
Аннотация: Kinetic indices of growth, polyhydroxyalkanoate (PHA) accumulation, and gas exchange were studied in a culture of the carbon monoxide-resistant hydrogen strain Ralstonia eutropha B-5786 grown on a gaseous substrate (GS) obtained by lignite gasification. The GS was shown to be suitable for PHA production. To increase the degree of GS consumption, various modes of gas supply to the culture were tested. Based on the results, an algorithm was developed for calculating and controlling gas-exchange parameters in the PHA-accumulating culture of Ralstonia eutropha, grown on a new GS allowing high polymer yields (up to 75%) and degrees of substrate utilization (up to 90%).

Scopus
Держатели документа:
Institute of Biophysics, Siberian Div. Russ. Acad. of Sci., Krasnoyarsk, 660036, Russian Federation
Siberian State Technol. University, Min. of Educ. of the Russ. Fed., Krasnoyarsk, 660049, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Voinov, N.A.

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18.

Dynamics of activity of the key enzymes of polyhydroxyalkanoate metabolism in Ralstonia eutropha B5786 / T. G. Volova [et al.] // Applied Biochemistry and Microbiology. - 2004. - Vol. 40, Is. 2. - P170-177, DOI 10.1023/B:ABIM.0000018921.04863.d5 . - ISSN 0003-6838
Кл.слова (ненормированные):
acetyl coenzyme A acyltransferase -- bacterial enzyme -- carbon -- carbon dioxide -- fructose -- hydrogen -- hydroxybutyrate dehydrogenase -- oxidoreductase -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- synthetase -- article -- bacterial metabolism -- carbon source -- catalysis -- controlled study -- degradation -- depolymerization -- enzyme activity -- enzyme analysis -- molecular dynamics -- nonhuman -- protein function -- Ralstonia eutropha -- recording -- statistical significance -- synthesis -- Bacteria (microorganisms) -- Ralstonia -- Wautersia eutropha
Аннотация: The dynamics of accumulation of polyhydroxybutyrate (PHB) and the activities of key enzymes of PHB metabolism (?-ketothiolase, acetoacetyl-CoA reductase, PHB synthase, D-hydroxybutyrate dehydrogenase, and PHB depolymerase) in the hydrogen bacterium Ralstonia eutropha B5786 were studied under various conditions of carbon nutrition and substrate availability. The highest activities of ?-ketothiolase, acetoacetyl-CoA reductase, and PHB synthase were recorded during acceleration of PHB synthesis. The activities of enzymes catalyzing PHB depolymerization (PHB depolymerase and D-hydroxybutyrate dehydrogenase) were low, being expressed only upon stimulated endogenous PHB degradation. The change of carbon source (CO2 or fructose) did not affect the time course of the enzyme activity significantly.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Gorbunova, O.V.; Zhila, N.O.

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19.

Dynamics of activity of the key enzymes of polyhydroxyalkanoate metabolism in Ralstonia eutropha / T. G. Volova [и др.] // Prikladnaia biokhimiia i mikrobiologiia. - 2004. - Vol. 40, Is. 2. - С. 201-209 . - ISSN 0555-1099
Кл.слова (ненормированные):
acetoacetyl coenzyme a reductase -- acetoacetyl-CoA reductase -- acetyl coenzyme A acyltransferase -- acyltransferase -- alcohol dehydrogenase -- carboxylesterase -- hydroxybutyrate dehydrogenase -- hydroxybutyric acid -- poly(3 hydroxyalkanoic acid) depolymerase -- poly(3-hydroxyalkanoic acid) depolymerase -- poly(3-hydroxyalkanoic acid) synthase -- polyhydroxyalkanoate synthase -- polymer -- article -- chemistry -- comparative study -- culture medium -- enzymology -- growth, development and aging -- metabolism -- Wautersia eutropha -- Acetyl-CoA C-Acyltransferase -- Acyltransferases -- Alcohol Oxidoreductases -- Carboxylic Ester Hydrolases -- Culture Media -- Cupriavidus necator -- Hydroxybutyrate Dehydrogenase -- Hydroxybutyrates -- Polymers
Аннотация: The dynamics of accumulation of polyhydroxybutyrate (PHB) and the activities of the key enzymes of PHB metabolism (beta-ketothiolase, acetoacetyl-CoA reductase, PHA synthase, D-hydroxybutyrate dehydrogenase, and PHA depolymerase) in the hydrogen bacterium Ralstonia eutropha B5786 were studied under various conditions of carbon nutrition and substrate availability. The highest activities of beta-ketothiolase, acetoacetyl-CoA reductase, and PHA synthase were recorded at the stage of acceleration of PHB synthesis. The activities of enzymes catalyzing PHB depolymerization (PHB depolymerase and D-hydroxybutyrate dehydrogenase) were low, being expressed only at stimulated endogenous PHB degradation. The change of carbon source (CO2 or fructose) did not cause any marked changes in the time course of enzyme activity.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Gorbunova, O.V.; Zhila, N.O.

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20.

Production of purified polyhydroxyalkanoates (PHAs) for applications in contact with blood / V. I. Sevastianov [et al.] // Journal of Biomaterials Science, Polymer Edition. - 2003. - Vol. 14, Is. 10. - P1029-1042, DOI 10.1163/156856203769231547 . - ISSN 0920-5063
Кл.слова (ненормированные):
?-hydroxy acids -- Endotoxins -- Hemocompatibility -- Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) -- Polyhydroxyalkanoates (PHAs) -- Polyhydroxybutyrate (PHB) -- bacterium lipopolysaccharide -- carbon -- complement -- copolymer -- hydroxyacid -- long chain fatty acid -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- valeric acid derivative -- adult -- article -- biofilm -- biotechnology -- blood analysis -- blood clotting -- blood compatibility -- cell function -- chemical analysis -- chemical composition -- complement activation -- concentration (parameters) -- controlled study -- gas chromatography -- hemostasis -- human -- human cell -- mass spectrometry -- micromorphology -- nonhuman -- priority journal -- purification -- quantitative analysis -- sampling -- synthesis -- thrombocyte adhesion -- Wautersia eutropha -- Biocompatible Materials -- Blood -- Blood Coagulation Tests -- Chromatography, Gas -- Complement Activation -- Cupriavidus necator -- Fatty Acids -- Humans -- Platelet Adhesiveness -- Polyesters -- Surface Properties
Аннотация: Samples of olyhydroxyalkanoates (PHAs), polyhydroxybutyrate (PHB) and copolymers poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) with 4 and 18 mol% hydroxyvalerate, synthesized by the bacteria Ralstonia eutropha B5786, were investigated. PHA films in contact with blood did not activate the hemostasis system at the level of cell response, but they did activate the coagulation system and the complement reaction. To detect biologically-active components in the PHAs, a detailed analysis of the composition of the polymers was conducted. Gas chromatography-mass spectrometry revealed long-chain fatty acids (FAs) in the tested PHAs. Their total concentration in the polymer ranged from tenths of mol% to 2-3 mol%, depending on the purification method. C16:0 constituted the largest proportion, up to 70%. Of the long-chain hydroxy acids, only ?-OH-C14:0 was detected and it did not exceed 0.06 mol%. The analysis of the hemocompatibility properties of the PHAs purified by a specialized procedure, including the quantitative and morphological estimation of platelets adherent to the surface of polymer films, the plasma recalcification time and complement activation studies, indicated that PHB and PHBV can be used in contact with blood. It has been found out that the lipopolysaccharides of bacteria producing PHAs, which contain mostly long-chain hydroxy acids, can be the factor activating the hemostasis systems. Thus, the technology of PHA purification must satisfy rather stringent specific requirements.

Scopus
Держатели документа:
Inst. of Transplantol. Artif. Organs, Russian Ministry of Health, Shchukinskaya 1, 123182 Moscow, Russian Federation
Inst. of Biophys. of Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sevastianov, V.I.; Perova, N.V.; Shishatskaya, E.I.; Kalacheva, G.S.; Volova, T.G.

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