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Stability of recombinant plasmids in transgenic microorganisms under different environmental conditions / L. Yu. Popova [и др.] // Mikrobiologiya. - 2001. - Vol. 70, Is. 6. - С. 796-803 . - ISSN 0026-3656
Кл.слова (ненормированные):
Antibiotic resistance -- Bioluminescence interferon -- Recombinant plasmids -- Transgenic microorganisms -- article -- Bacillus subtilis -- culture medium -- Escherichia coli -- genetic engineering -- genetic recombination -- genetics -- R factor -- Bacillus subtilis -- Culture Media -- Escherichia coli -- Genetic Engineering -- R Factors -- Recombination, Genetic
Аннотация: The copy number of R plasmids weakly depends on the selective pressure of the respective antibiotic but does depend on the physiology of the host species and the type of plasmids and cloned genes, whose expression leads to a further load on the biosynthetic apparatus of cells. The last factor is critical in the maintenance of recombinant plasmids in transgenic microorganisms.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Popova, L.Yu.; Maksimova, E.E.; Lobova, T.I.; Kargatova, T.V.; Boyandin, A.N.; Krylova, T.Yu.; Pechurkin, N.S.

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Effect of salinity on the adaptive capacity of recombinant strains of escherichia coli and bacillus subtilis / A. N. Boyandin [и др.] // Mikrobiologiya. - 2000. - Vol. 69, Is. 2. - С. 243-247 . - ISSN 0026-3656
Кл.слова (ненормированные):
Genetically modified microorganisms -- Salinity -- Salt resistance -- sodium chloride -- adaptation -- article -- Bacillus subtilis -- electrolyte balance -- Escherichia coli -- genetic recombination -- genetics -- physiology -- Adaptation, Biological -- Bacillus subtilis -- Escherichia coli -- Recombination, Genetic -- Sodium Chloride -- Water-Electrolyte Balance
Аннотация: Effect of different concentrations of salts on natural and recombinant strains of Bacillus subtilis and Escherichia coli was studied. The recombinant strain of B. subtilis was found to be more osmotolerant than the wild-type strain of this bacterium, whereas the opposite situation was observed for the recombinant and wildtype strains of E. coli. Some salts exerted a bacteriostatic effect on E. coli and B. subtilis. The adaptive capacity of recombinant strains depended on the number of plasmid copies in the cells. The introduction of recombinant bacteria into model ecosystems resulted in the generation of their variants with increased osmotolerance.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Lobova, T.I.; Yu Krylova, T.; Kargatova, T.V.; Yu Popova, L.; Pechurkin, N.S.

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Population heterogeneity of plasmid-bearing and plasmid-free bacillus subtilis strains under different environmental conditions / T. Yu. Krylova [и др.] // Mikrobiologiya. - 2000. - Vol. 69, Is. 2. - С. 270-275 . - ISSN 0026-3656
Кл.слова (ненормированные):
Bacillus subtilis -- Heterogeneity -- Introduction -- Microcosm -- Recombinant plasmid -- article -- Bacillus subtilis -- culture medium -- genetic recombination -- genetics -- plasmid -- Bacillus subtilis -- Culture Media -- Plasmids -- Recombination, Genetic
Аннотация: The population heterogeneity of recombinant and plasmid-free Bacillus subtilis strains introduced into aquatic microcosms was studied. After introduction, the population of the plasmid-free strain B. subtilis 2335 in microcosms has long been represented by both vegetative cells and spores, whereas, already ten days after introduction, the population of the recombinant strain B. subtilis 2335/105 (Km rInf +) was represented only by spores. The number of plasmid copies in the spore isolates of the recombinant strain was the same as before introduction, but the plasmid abundance in the vegetative isolates of this strain decreased. The isolates of B. subtilis 2335/105 obtained from microcosms and the variants of this strain obtained by ten successive subcultures on M9 and 0.1 ? M9 media with and without kanamycin (Km) differed in the number of plasmid copies, Km resistance, and maximum biomass yield during batch cultivation. Irrespective of the presence of Km, more than 50% of the variants subcultured on M9 medium showed reduced plasmid abundance. At the same time, about 70% of the variants subcultured on 0.1 ? M9 medium with Km and 90% of the variants subcultured on the same medium without Km retained the initial number of plasmid copies. The variants subcultured on media with Km retained the initial biomass level. In more than 70% of the variants isolated from media without Km, the biomass yield increased.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Krylova, T.Yu.; Popova, L.Yu.; Pechurkin, N.S.; Kashperova, T.A.; Belyavskaya, V.A.

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Phenotypic variability of the population of a recombinant luminescent strain of escherichia coli in aqueous microcosms / T. V. Kargatova [и др.] // Mikrobiologiya. - 1997. - Vol. 66, Is. 1. - С. 101-106 . - ISSN 0026-3656
Кл.слова (ненормированные):
article -- Escherichia coli -- genetic recombination -- genetics -- luminescence -- microbiology -- penicillin resistance -- phenotype -- plasmid -- Ampicillin Resistance -- Escherichia coli -- Luminescent Measurements -- Phenotype -- Plasmids -- Recombination, Genetic -- Water Microbiology
Аннотация: The behavior of Escherichia coli Z905, carrying a recombinant plasmid pPHL7 with genes determining ampicillin resistance and bacterial luminescence, and the efficiency of expression of cloned genes were studied after introduction of the strain into model aqueous ecosystems with different trophic chain lengths. The E. coli Z905 variants isolated from ecosystems after different periods of time were found to vary in their resistance to ampicillin (from 50 to 0.05 ?g/ml) and in the intensity of bioluminescence. An increase in the concentration of the selective factor (ampicillin) or in the extent of the aqueous microcosm blooming restored the expression of the recombinant plasmid genes in some clones.

Scopus
Держатели документа:
Institute uf Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kargatova, T.V.; Maksimova, E.E.; Popova, L.Yu.; Bril'Kov, A.V.; Pechurkin, N.S.

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Introduction and long-term storage of recombinant luminescent Escherichia coli strain Z905 in laboratory water microecosystems / L. I. Popova [и др.] // Izvestiia Akademii nauk. Seriia biologicheskaia / Rossiiskaia akademiia nauk. - 1998. - Is. 6. - С. 670-677 . - ISSN 1026-3470
Кл.слова (ненормированные):
article -- bacterial gene -- chemoluminescence -- Escherichia coli -- gene expression -- genetic recombination -- genetics -- laboratory -- microbiology -- Chemiluminescent Measurements -- Escherichia coli -- Gene Expression -- Genes, Bacterial -- Laboratories -- Recombination, Genetic -- Water Microbiology
Аннотация: We studied preservation of recombinant Escherichia coli strain Z905 (AprLux+) in liquid microecosystems (LME) after the introduction. E. coli cells were shown to remain viable and preserve the ability to express the cloned lux genes for a long time (more than a year) in LME. The majority of the clones have reduced efficiency of the expression due to either changed regulation of the lux operon or decreased number of copies of the plasmid. These mechanisms could be realized either independently or simultaneously depending on LME conditions. We have exposed the major factors affecting the metabolic activity of the E. coli strain Z905 (AprLux+) introduced into model ecosystems and the level of expression of the cloned genes.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Popova, L.I.; Maksimova, E.E.; Kargatova, T.V.; Bril'kov, A.V.; Pechurkin, N.S.

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