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1.


   
    Vertical stratification of physical, chemical and biological components in two saline lakes Shira and Shunet (South Siberia, Russia) / A. G. Degermendzhy [et al.] // Aquatic Ecology. - 2010. - Vol. 44, Is. 3. - P619-632, DOI 10.1007/s10452-010-9336-6 . - ISSN 1386-2588
Кл.слова (ненормированные):
Ciliates -- Cryptomonas -- Fishless lakes -- Gammarus -- Mathematical modelling -- Meromictic lakes -- Phytoplankton -- Stratification -- Sulphur bacteria -- amphipod -- bacterium -- biomass -- ciliate -- ecosystem modeling -- flagellate -- meromictic lake -- microbial community -- numerical model -- physicochemical property -- phytoplankton -- population density -- saline lake -- salinity -- stratification -- thermocline -- trophic interaction -- vertical profile -- zooplankton -- Khakassia -- Lake Shira -- Lake Shunet -- Russian Federation -- Siberia -- Amphipoda -- Bacteria (microorganisms) -- Ciliophora -- Copepoda -- Cryptomonas -- Cryptomonas sp. -- Gammaridae -- Gammarus -- Gammarus lacustris -- Phytomastigophorea -- Protista -- Rotifera
Аннотация: A feature of meromictic lakes is that several physicochemical and biological gradients affect the vertical distribution of different organisms. The vertical stratification of physical, chemical and biological components in saline, fishless meromictic lakes Shira and Shunet (Siberia, Russia) is quite different mainly because both mean depth and maximum depth of lakes differ as well as their salinity levels differ. The chemocline of the Lake Shira, as in many meromictic lakes, is inhabited by bacterial community consisting of purple sulphur and heterotrophic bacteria. As the depth of the chemocline is variable, the bacterial community does not attain high densities. The mixolimnion in Lake Shira, which is thermally stratified in summer, also creates different habitat for various species. The distribution of phytoplankton is non-uniform with its biomass peak in the metalimnion. The distribution of zooplankton is also heterogeneous with rotifers and juvenile copepods inhabiting the warmer epilimnion and older copepods found in the cold but oxic hypolimnion. The amphipod Gammarus lacustris which can be assigned to the higher trophic link in the fishless lake's ecosystem, such as Lake Shira, is also distributed non-uniformly, with its peak density generally observed in the thermocline region. The chemocline in Lake Shunet is located at the depth of 5 m, and unlike in Lake Shira, due to a sharp salinity gradient between the mixolimnion and monimolimnion, this depth is very stable. The mixolimnion in Lake Shunet is relatively shallow and the chemocline is inhabited by (1) an extremely dense bacterial community; (2) a population of Cryptomonas sp.; and (3) ciliate community comprising several species. As the mixolimnion of Lake Shunet is not thermally stratified for long period, the phytoplankton and zooplankton populations are not vertically stratified. The gammarids, however, tend to concentrate in a narrow layer located 1-2 m above the chemocline. We believe that in addition to vertical inhomogeneities of both physicochemical parameters, biological and physical factors also play a role in maintaining these inhomogeneities. We conclude that the stratified distributions of the major food web components will have several implications for ecosystem structure and dynamics. Trophic interactions as well as mass and energy flows can be significantly impacted by such heterogeneous distributions. Species spatially separated even by relatively short distances, say a few centimetres will not directly compete. Importantly, we demonstrate that not only bacteria, phytoflagellates and ciliate tend to concentrate in thin layers but also larger-sized species such Gammarus (amphipods) can also under certain environmental conditions have stratified distribution with maxima in relatively thin layer. As the vertical structure of the lake ecosystem is rather complex in such stratified lakes as ours, the strategy of research, including sampling techniques, should consider potentially variable and non-homogeneous distributions. В© 2010 The Author(s).

Scopus
Держатели документа:
Institute of Biophysics SB RAS, 660036 Krasnoyarsk, Akademgorodok, Russian Federation
Siberian Federal University, Svobodnyi 79, 660041 Krasnoyarsk, Russian Federation
Netherlands Environmental Assessment Agency (PBL), P.O. Box 303, 3720 AH Bilthoven, Netherlands
Department of Aquatic Ecology, Netherlands Institute of Ecology (NIOO-KNAW), 3631 AC Nieuwersluis, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Degermendzhy, A.G.; Zadereev, E.S.; Rogozin, D.Y.; Prokopkin, I.G.; Barkhatov, Y.V.; Tolomeev, A.P.; Khromechek, E.B.; Janse, J.H.; Mooij, W.M.; Gulati, R.D.

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2.


   
    A one-dimensional model of vertical stratification of Lake Shira focussed on winter conditions and ice cover / S. N. Genova [et al.] // Aquatic Ecology. - 2010. - Vol. 44, Is. 3. - P571-584, DOI 10.1007/s10452-010-9327-7 . - ISSN 1386-2588
Кл.слова (ненормированные):
Convective mixed layer -- Ice formation -- Ice melting -- Simplified model -- Winter profiles -- anoxic conditions -- hydrogen sulfide -- ice cover -- inhomogeneity -- meromictic lake -- numerical model -- one-dimensional modeling -- pycnocline -- salinity -- seasonality -- stratification -- vertical profile -- water column -- water temperature -- Khakassia -- Lake Shira -- Russian Federation
Аннотация: In meromictic lakes such as Lake Shira, horizontal inhomogeneity is small in comparison with vertical gradients. To determine the vertical distribution of temperature, salinity, and density of water in a deep zone of a Lake Shira, or other saline lakes, a one-dimensional (in vertical direction) mathematical model is presented. A special feature of this model is that it takes into account the process of ice formation. The model of ice formation is based on the one-phase Stefan problem with the linear temperature distribution in the solid phase. A convective mixed layer is formed under an ice cover due to salt extraction in the ice formation process. To obtain analytical solutions for the vertical distribution of temperature, salinity, and density of water, we use a scheme of vertical structure in the form of several layers. In spring, the ice melts as top and bottom. These processes are taken into account in the model. The calculated profiles of salinity and temperature of Shira Lake are in good agreement with field measurement data for each season. Additionally, we focussed on the redox zone, which is the zone in which the aerobic layers of a water column meet the anaerobic ones. Hyperactivity of plankton communities is observed in this zone in lakes with hydrogen sulphide monimolimnion, and Lake Shira is among them. The location of the redox zone in the lake, which is estimated from field measurements, coincides with a sharp increase in density (the pycnocline) during autumn and winter. During spring and summer, the redox zone is deeper than the pycnocline. The location of pycnocline calculated with the hydro physical model is in good agreement with field measurement data. В© 2010 Springer Science+Business Media B.V.

Scopus
Держатели документа:
Institute of Computational Modeling SB RAS, Akademgorodok 50/44, Krasnoyarsk 660036, Russian Federation
Krasnoyarsk, Siberian Federal University, Institute of Mathematics, Svobodny 79, Krasnoyarsk 660041, Russian Federation
Institute of Biophysics SB RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Federal University, Institute of Mathematics, Svobodny 79, Krasnoyarsk 660041, Russian Federation
Netherlands Institute of Ecology, Center for Limnology, Rijksstraatweg 6, Nieuwersluis 3631 AC, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Genova, S.N.; Belolipetskii, V.M.; Rogozin, D.Y.; Degermendzhy, A.G.; Mooij, W.M.

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3.


   
    Some generalizations based on stratification and vertical mixing in meromictic Lake Shira, Russia, in the period 2002-2009 / D. Y. Rogozin [et al.] // Aquatic Ecology. - 2010. - Vol. 44, Is. 3. - P485-496, DOI 10.1007/s10452-010-9328-6 . - ISSN 1386-2588
Кл.слова (ненормированные):
1-D model -- Meromixis -- Mixolimnion -- Oxic-anoxic interface -- Thermocline -- Weather conditions -- brackish water -- meromictic lake -- numerical model -- one-dimensional modeling -- overturn -- physicochemical property -- salinity -- seasonality -- stratification -- temperate environment -- vertical mixing -- water temperature -- weather -- Khakassia -- Lake Shira -- Russian Federation
Аннотация: In a brackish, temperate, 24-m-deep Lake Shira, the profiles of salinity, temperature, oxygen and sulfide concentrations were measured on a seasonal basis from 2002 to 2009. The lake was shown to be meromictic with autumnal overturn restricted to mixolimnion. The depth of mixolimnion and position of oxic-anoxic interface varied annually. The spring mixing processes contribute to the formation of mixolimnion in autumn. The exceptionally windy spring of 2007 caused the deepening of mixolimnion in the winter of 2008. The winter position of oxic-anoxic interface was affected by the position of lower boundary of mixolimnion in all winters. The salinity in the winter mixolimnion increased compared with the autumn because of freezing out of salts from the upper water layers meters during ice formation and their dissolution in water below. The profiles of salinity and temperature were simulated by the mathematical 1-D model of temperature and salinity conditions taking into account ice formation. The simulated profiles generally coincided with the measured ones. The coincidence implies that simplified one-dimensional model can be applied to roughly describe salinity and density profiles and mixing behavior of Lake Shira. В© 2010 The Author(s).

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok 50-50, 660036 Krasnoyarsk, Russian Federation
Siberian Federal University, Svobodny 79, 660071 Krasnoyarsk, Russian Federation
Institute of Computational Modeling of Siberian Branch of Russian Academy of Sciences, Akademgorodok 50-50, 660036 Krasnoyarsk, Russian Federation
The Netherlands Institute of Ecology, Center for Aquatic Ecology, Nieuwersluis, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Rogozin, D.Y.; Genova, S.N.; Gulati, R.D.; Degermendzhy, A.G.

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4.


   
    Effect of salinity on the biochemical composition of the alga Botryococcus braunii Kutz IPPAS H-252 / N. O. Zhila, G. S. Kalacheva, T. G. Volova // Journal of Applied Phycology. - 2011. - Vol. 23, Is. 1. - P47-52, DOI 10.1007/s10811-010-9532-8 . - ISSN 0921-8971
Кл.слова (ненормированные):
Botryococcus -- Fatty acid composition -- Lipid content -- Salinity -- algae -- Botryococcus -- Botryococcus braunii
Аннотация: The effect of 0.3 and 0.7 M NaCl on biomass yield, total nitrogen content, intracellular lipid content, and fatty acid profile of the lipids of the alga Botryococcus braunii IPPAS H-252 in different phases of the culture cycle was studied. The presence of sodium chloride in the medium inhibited the growth of algal cells for the first 3 days of the experiment, causing a decrease in total nitrogen, enhanced synthesis of triacylglycerols, and considerable changes in the lipid fatty acid profile: decreases in polyenoic acid contents (from 68.34% to 29.38% and 12.8%) and proportions of long-chain saturated acids (from 0.53% to 5.3% and 14.13% of the total fatty acids) at 0.3 M NaCl and 0.7 M NaCl, respectively. In later phases of the culture, at 0.3 M NaCl, the content of polyenoic acids rose to the values characteristic of the active growth phase of this alga. At 0.7 M NaCl, the proportion of polyenoic acids grew less significantly, but biomass concentration and total nitrogen increased, similarly to the experiment with 0.3 M NaCl. В© 2010 Springer Science+Business Media B.V.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, Akademgorodok, 50, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, 79 Svobodny Prospect, Krasnoyarsk 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zhila, N.O.; Kalacheva, G.S.; Volova, T.G.

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5.


   
    Effect of photosynthetically active radiation, salinization, and type of nitrogen nutrition on growth of Salicornia europaea plants / S. A. Ushakova [et al.] // Russian Journal of Plant Physiology. - 2006. - Vol. 53, Is. 6. - P785-792, DOI 10.1134/S1021443706060094 . - ISSN 1021-4437
Кл.слова (ненормированные):
NaCl salinization -- Photosynthetically active radiation -- Productivity -- Salicornia europaea -- Types of nitrogen nutrition -- Salicornia europaea
Аннотация: Effects of various combinations of nutrient solution salinity (0.3, 171, and 342 mM NaCl), photosynthetically active radiation (PAR) of 600 or 1150 ?mol/(m2 s), and type of nitrogen nutrition (amide-N or nitrate-N) on the productivity and the content of accumulated mineral nutrients and free amino acids were studied in Salicornia europaea plants. At PAR of 600 ?mol/(m2 s), plant productivity increased with elevation of salinity level; at 1150 ?mol/(m2 s), the maximum productivity was observed in the plants grown at 171 mM of NaCl. The content of free amino acids in shoots, regardless of PAR, decreased with growing salinity level, whereas Na content, on the opposite, increased. Glutamic acid, rather than proline, was shown to be the main organic osmolyte in this plant species. Comparison of the productivity of plants grown on solutions with amide (urea) or nitrate nitrogen showed that higher biomass accumulation was achieved in the former case. В© 2006 Nauka/Interperiodica.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Ushakova, S.A.; Kovaleva, N.P.; Tikhomirova, N.A.; Gribovskaya, I.V.; Kolmakova, A.A.

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6.


   
    Effect of NaCl concentration on productivity and mineral composition of Salicornia europaea as a potential crop for utilization NaCl in LSS / S. A. Ushakova [et al.] // Advances in Space Research. - 2005. - Vol. 36, Is. 7. - P1349-1353, DOI 10.1016/j.asr.2004.09.017 . - ISSN 0273-1177
Кл.слова (ненормированные):
Life support system -- NaCl -- Salicornia europaea -- Space biology -- Calcium -- Concentration (process) -- Minerals -- Photosynthesis -- Plants (botany) -- Salinity measurement -- Sodium chloride -- Vegetation -- Life support systems -- Liquid wastes -- NaCl -- Salicornea europea -- Space biology -- Space research
Аннотация: The accumulation of solid and liquid wastes in manmade ecosystems presents a problem that has not been efficiently solved yet. Urine, containing NaCl, are part of these products. This is an obstacle to the creation of biological systems with a largely closed material cycling, because the amount of solid and liquid wastes in them must be reduced to a minimum. A possible solution to the problem is to select plant species capable of utilizing sufficiently high concentrations of NaCl, edible for humans, and featuring high productivity. Until recently, the life support systems have included the higher plants that were either sensitive to salinization (wheat, many of the legumes, carrot, potato, maize) or relatively salt-resistant (barley, sugar beet, spinach). Salicomia europaea, whose above-ground part is fully edible for humans, is one of the most promising candidates to be included in life support systems. It is reported in the literature that this plant is capable of accumulating up to 50% NaCl (dry basis). Besides, excessive accumulation of sodium ions should bring forth a decrease in the uptake of potassium ions and other biogenic elements. The aim of this work is to study the feasibility of using S. europaea plants in growth chambers to involve NaCl into material cycling. Plants were grown in vegetation chambers at the irradiance of 100 or 150 W/m2 PAR (photosynthetically active radiation) and the air temperature 24 В°C, by two methods. The first method was to grow the plants on substrate - peat. The peat was supplemented with either 3% NaCl (Variant 1) or 6% NaCl (Variant 2) of the oven-dry mass of the peat. The second method was to grow the plants in water culture, using the solution with a full complement of nutrients, which contained 0.0005% of NaCl, 1% or 2%. The study showed that the addition of NaCl to the substrate or to the solution resulted in the formation of more succulent plants, which considerably increased their biomass. The amount of NaCl uptake was the highest in the plants grown in water culture, 2.6 g per plant. As the sodium uptake increased, the consumption of potassium and the sum of the reduced N forms decreased twofold. The uptake of calcium and magnesium by plants decreased as the NaCl concentration increased; the smallest amounts were taken up by S. europaea grown in water culture. Salinity had practically no effect on the uptake of phosphorus and sulfur. Thus, S. europaea is a promising candidate to be included in life support systems; of special interest is further research on growing these plants in water culture. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Science, Siberian Branch, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Ushakova, S.A.; Kovaleva, N.P.; Gribovskaya, I.V.; Dolgushev, V.A.; Tikhomirova, N.A.

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7.


   
    Feeding spectra of Arctodiaptomus salinus (Calanoida, Copepoda) using fatty acid trophic markers in seston food in two salt lakes in South Siberia (Khakasia, Russia) / A. P. Tolomeev [et al.] // Aquatic Ecology. - 2010. - Vol. 44, Is. 3. - P513-530, DOI 10.1007/s10452-010-9331-y . - ISSN 1386-2588
Кл.слова (ненормированные):
Arctodiaptomus salinus -- Brackish lakes -- Fatty acids -- Feeding spectra -- Salinity adaptation -- adaptation -- bacterium -- brackish water -- ciliate -- comparative study -- crustacean -- cyanobacterium -- fatty acid -- feeding behavior -- flagellate -- food selection -- green alga -- lipid -- physicochemical property -- picoplankton -- saline lake -- salinity -- seasonality -- seston -- Khakassia -- Russian Federation -- Siberia -- Animalia -- Arctodiaptomus salinus -- Bacillariophyta -- Bacteria (microorganisms) -- Calanoida -- Chlorophyta -- Ciliophora -- Copepoda -- Cryptomonas -- Cryptophyta -- Cyanobacteria -- Mastigophora (flagellates) -- Proteobacteria
Аннотация: During two vegetation seasons (2004-2005), we compared feeding spectra of Arctodiaptomus salinus (Calanoida, Copepoda) populations inhabiting two neighboring salt lakes, Shira and Shunet, Khakasia, Russia, using fatty acid (FA) trophic markers. Sestonic FA composition in two lakes moderately differed, whereas levels of diatom FA markers were higher in Lake Shunet and of Cyanobacteria and green algae markers in Lake Shira. In general, markers in storage lipids-triacylglycerols (TAG) of A. salinus-reflected the differences in sestonic composition of the two lakes. Nevertheless, TAG fraction was also enriched by FA trophic markers of the minor components of seston, which were selectively ingested by the animals. In Lake Shira, A. salinus had significantly higher concentrations of bacterial FA markers in TAG. In Lake Shunet, TAG of A. salinus contained significantly higher relative amounts of 18:4?3, 18:5?3 and C22 polyunsaturated fatty acids (PUFA), which indicated marked contribution of cryptophytes or (and) flagellates into the diet. Laboratory experiments showed feeding on Cryptomonas and sulfur purple bacteria in Lake Shunet and ciliates and colonial picoplankton in both lakes, and generally confirmed the differences in FA trophic markers in A. salinus between the lakes. The two populations of A. salinus markedly differed in levels of essential long-chain PUFA, eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, although the levels of these FA in seston were quite similar between the two lakes. The higher levels of the essential ?3 PUFA in A. salinus in Lake Shunet may be an adaptive response of the animals to a vertical stratification of physico-chemical conditions and significantly higher salinity levels at the boundary of adjacent bottom layer in this lake. В© 2010 Springer Science+Business Media B.V.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russian Federation
Siberian Federal University, Svobodny av. 79, 660041 Krasnoyarsk, Russian Federation
Netherlands Institute of Ecology, Department of Aquatic Ecology, Rijksstraatweg 6, 3631 AC Nieuwersluis, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tolomeev, A.P.; Sushchik, N.N.; Gulati, R.D.; Makhutova, O.N.; Kalacheva, G.S.; Zotina, T.A.

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8.


   
    Characteristics of aboveground phytomass accumulation in halophytic plant communities under the conditions of different soil salinities / N. A. Slyusar, N. S. Pechurkin, T. M. Zorkina // Doklady Biological Sciences. - 2010. - Vol. 432, Is. 1. - P194-197, DOI 10.1134/S0012496610030087 . - ISSN 0012-4966
Кл.слова (ненормированные):
fresh water -- article -- biomass -- ecosystem -- halophyte -- physiology -- salinity -- soil -- Biomass -- Ecosystem -- Fresh Water -- Salinity -- Salt-Tolerant Plants -- Soil

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50/50, Krasnoyarsk 660036, Russian Federation
Khakas State University, ul. Lenina 90, Abakan 655017, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Slyusar, N.A.; Pechurkin, N.S.; Zorkina, T.M.

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9.


   
    Survival and alteration of the plasmid-containing microorganism Escherichia coli Z905/pPHL7 introduced into manmade closed aquatic microcosms / A. N. Boyandin [et al.] // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1763-1768, DOI 10.1016/S0273-1177(03)00118-2 . - ISSN 0273-1177
Кл.слова (ненормированные):
Cells -- Escherichia coli -- Salts -- Recombinant plamid -- Space research -- ampicillin -- chloride -- inorganic salt -- magnesium -- mineral -- potassium -- sodium -- sulfate -- genetically modified organism -- article -- bacterial count -- chemoluminescence -- culture medium -- drug effect -- Escherichia coli -- genetics -- growth, development and aging -- microbiology -- microclimate -- penicillin resistance -- plasmid -- Russian Federation -- transgenic organism -- Ampicillin -- Ampicillin Resistance -- Chemiluminescent Measurements -- Chlorides -- Colony Count, Microbial -- Culture Media -- Ecological Systems, Closed -- Escherichia coli -- Magnesium -- Minerals -- Organisms, Genetically Modified -- Plasmids -- Potassium -- Russia -- Salts -- Sodium -- Sulfates -- Water Microbiology
Аннотация: It has been demonstrated that the transgenic microorganism Escherichia coli Z905/pPHL7 (Ap'Lux+) can exist for a long time at an elevated concentration of mineral salts. The microorganism was introduced into microcosms with sterile brackish water (salinity variable from 21 to 22 g 1-1) taken from Lake Shira (Khakasia, Russia). The survivof the microorganism was estimated both by measuring the growth of the colonies on solid nutrient media and by the bioluminescence exhibited by the transgenic strain in samples from the microcosms and in the enrichment culture with the added selective factor - ampicillin (50 ?g/ml). In the enrichment culture, the bioluminescent signal was registered through the 160-day experiment. It has been shown that in the closed microcosms with brackish water the E. coli strain becomes heterogeneous in its ampicillin resistance. The populations of the transgenic strain were mainly represented by isolates able to persist in the medium containing 50 ?g/ml, but there were also the cells (about 10%) with the threshold of ampicillin resistance not more than 0.05 ?g/ml. Thus, it was shown that in the microcosms with brackish water and in the absence of the selective factor the transgenic strain survives and retains the recombinant plasmid. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Lobova, T.I.; Popova, L.Yu.; Pechurkin, N.S.

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10.


   
    Geographical and seasonal distribution of multiple antibiotic resistance of heterotrophic bacteria of Lake Shira / T. I. Lobova [et al.] // Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - P299-307, DOI 10.1023/A:1015618820450 . - ISSN 1386-2588
Кл.слова (ненормированные):
Ampicillin -- Brackish lake -- Halotolerance -- Heterotrophic bacteria -- Horizontal gene transfer -- Kanamycin -- Multiple antibiotic resistance -- antibiotics -- bacterium -- drug resistance -- geographical distribution -- saline lake -- salinity tolerance -- seasonal variation -- Russian Federation -- Bacteria (microorganisms)
Аннотация: From 1996 to 1999 heterotrophic bacteria of the brackish-water Lake Shira (Republic of Khakasia, Russia) were studied to understand the seasonal dynamics of their antibiotic resistance. During the winter, these bacteria were represented primarily by forms that could not be cultured and were psychrotolerant. In the summer period, heterotrophic, mesophilic bacteria increased in number. The percentages of isolates with multiple, antibiotic resistance isolated from the lake region near the resort area of the lake were 2-3 times higher than those from the central part of the lake. A decline in the bacterial numbers with multiple antibiotic resistance was observed during the cold period (February-March). Various mechanisms of multiple, antibiotic resistance of heterotrophic bacteria isolated from Shira lake are discussed.

Scopus
Держатели документа:
Institute of Biophysics of SB RAS, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Lobova, T.I.; Maksimova, E.Ye.; Popova, L.Yu.; Pechurkin, N.S.

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11.


   
    Effect of salinity on the adaptive capacity of recombinant strains of Escherichia coli and Bacillus subtilis / A. N. Boyandin [et al.] // Microbiology. - 2000. - Vol. 69, Is. 2. - P196-199 . - ISSN 0026-2617
Кл.слова (ненормированные):
Genetically modified microorganisms -- Salinity -- Salt resistance
Аннотация: The effect of different concentrations of salts on natural and recombinant strains of Bacillus subtilis and Escherichia coli was studied. The recombinant strain of B. subtilis was found to be more osmotolerant than the wild-type strain of this bacterium, whereas the opposite situation was observed for the recombinant and wild-type strains of E. coli. Some salts exerted a bacteriostatic effect on E. coli and B. subtilis. The adaptive capacity of recombinant strains depended on the number of plasmid copies in the cells. The introduction of recombinant bacteria into model ecosystems resulted in the generation of their variants with increased osmotolerance. В© 2000 MAIK "Nauka/Interperiodica".

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Lobova, T.I.; Krylova, T.Yu.; Kargatova, T.V.; Popova, L.Yu.; Pechurkin, N.S.

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12.


   
    Effect of salinity on the adaptive capacity of recombinant strains of escherichia coli and bacillus subtilis / A. N. Boyandin [и др.] // Mikrobiologiya. - 2000. - Vol. 69, Is. 2. - С. 243-247 . - ISSN 0026-3656
Кл.слова (ненормированные):
Genetically modified microorganisms -- Salinity -- Salt resistance -- sodium chloride -- adaptation -- article -- Bacillus subtilis -- electrolyte balance -- Escherichia coli -- genetic recombination -- genetics -- physiology -- Adaptation, Biological -- Bacillus subtilis -- Escherichia coli -- Recombination, Genetic -- Sodium Chloride -- Water-Electrolyte Balance
Аннотация: Effect of different concentrations of salts on natural and recombinant strains of Bacillus subtilis and Escherichia coli was studied. The recombinant strain of B. subtilis was found to be more osmotolerant than the wild-type strain of this bacterium, whereas the opposite situation was observed for the recombinant and wildtype strains of E. coli. Some salts exerted a bacteriostatic effect on E. coli and B. subtilis. The adaptive capacity of recombinant strains depended on the number of plasmid copies in the cells. The introduction of recombinant bacteria into model ecosystems resulted in the generation of their variants with increased osmotolerance.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Lobova, T.I.; Yu Krylova, T.; Kargatova, T.V.; Yu Popova, L.; Pechurkin, N.S.

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13.


   
    Seasonal and centennial cycles of carbonate mineralisation during the past 2500 years from varved sediment in Lake Shira, South Siberia / I. Kalugin [et al.] // Quaternary International. - 2013. - Vol. 290-291. - P245-252, DOI 10.1016/j.quaint.2012.09.016 . - ISSN 1040-6182
Кл.слова (ненормированные):
biochemistry -- carbonate -- environmental factor -- lacustrine deposit -- mineralization -- paleoenvironment -- pH -- salinity -- sediment chemistry -- temperature effect -- varve -- Khakassia -- Lake Shira -- Russian Federation -- Siberia
Аннотация: Shira Lake is a good representative object for detail weather-climate modeling due to its local hydroclimatic information and annually laminated bottom sediments. Carbonate biochemical mineralization in Shira Lake shows not only seasonal but also centennial pulses over each 450-500 years. An age-depth model has been built by layer counting combined with 137Cs and 14C isotopic dates. Thermodynamic estimation of the rock-water multisystem in conformity with local conditions and source matter provides grounds for interpretation of measured geochemical parameters in sediments including environmental indicators such as temperature, salinity, and pH. Scanning X-ray fluorescence for sub-millimeter microstratigraphic study of varves connects geochemical oscillations of microelement content (Rb, Ti, Ca, Sr, Br, XRD) with seasonality, as well as revealing centennial pulses of environmental change. Quantitative environmental reconstruction using multiple regression for precipitation calibrated by hydrometeorological time series year by year was carried out for the lake over the last 2500 years. В© 2012 Elsevier Ltd and INQUA.

Scopus
Держатели документа:
Siberian Branch of Russian Academy of Science, Institute of Geology and Mineralogy, Prospekt akademika Koptyuga 3, 630090 Novosibirsk, Russian Federation
Institute of Biophysics, Siberian Branch of Russian Academy of Science, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kalugin, I.; Darin, A.; Rogozin, D.; Tretyakov, G.

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14.


   
    Effect of salinity on the growth of the cyanobacterium Spirulina platensis in monoculture and mixed cultures [Text] / A. Y. Bolsunovskii, T. A. Zotina // Microbiology. - 1996. - Vol. 65, Is. 3. - P371-372. - Cited References: 5 . - 2. - ISSN 0026-2617
РУБ Microbiology

Аннотация: The effect of NaCl concentrations on the growth of the cyanobacterium Spirulina platensis in monoculture and in mixed culture with the green halophilic alga Dunaliella salina was studied. The,growth rate of S. platensis in monoculture did not change within the salinity range from 1 to 20 g/l NaCl. With an increase in salinity, the growth rate declined, and, at 70 g/l NaCl, the cyanobacteria died. The response of S. platensis to changes in salinity in mixed culture with D. salina did not differ from that observed in monoculture. At a concentration of 50 g/l NaCl these species of microorganisms could coexist.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovskii, A.Y.; Zotina, T.A.

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15.


   
    Salt-dependent inhibition of light emitting of the luminescent microorganism Escherichia coli Z9051 [Текст] / A. N. Boyandin, L. Y. Popova // Biofizika. - 2001. - Vol. 46, Is. 2. - С. 251-255. - Cited References: 12 . - 5. - ISSN 0006-3029
РУБ Biophysics

Кл.слова (ненормированные):
bacterial luminescence -- recombinant plasmid -- salt concentration
Аннотация: The influence of some mineral salts on the recombinant strain Escherichia coli Z9051 was investigated. It was shown that the composition (NaCl, Na2SO4, MgCl2 and MgSO4) and concentration (5 and 10%) of the salts substantially affect the expression of genes for the luminescence system of fight-emitting bacteria cloned in the plasmid under the control of the lac-promoter. In some cases, the luminescence level of the microorganism in the presence of salts was similar to the luminescence level under catabolite repression by glucose, the more strong influence of the salts exceeding the effect of catabolite repression. The possibility of adaptation of the genetically modified microorganism to the salinity factor is discussed.

Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Popova, L.Y.

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16.


   
    Salt-dependent inhibition of light emitting of the luminescent microorganism Escherichia coli Z9051 [Текст] / A. N. Boyandin, L. Y. Popova // Biofizika. - 2001. - Vol. 46, Is. 2. - P. 251-255. - Cited References: 12 . - ISSN 0006-3029
РУБ Biophysics

Кл.слова (ненормированные):
bacterial luminescence -- recombinant plasmid -- salt concentration
Аннотация: The influence of some mineral salts on the recombinant strain Escherichia coli Z9051 was investigated. It was shown that the composition (NaCl, Na2SO4, MgCl2 and MgSO4) and concentration (5 and 10%) of the salts substantially affect the expression of genes for the luminescence system of fight-emitting bacteria cloned in the plasmid under the control of the lac-promoter. In some cases, the luminescence level of the microorganism in the presence of salts was similar to the luminescence level under catabolite repression by glucose, the more strong influence of the salts exceeding the effect of catabolite repression. The possibility of adaptation of the genetically modified microorganism to the salinity factor is discussed.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Popova, L.Y.

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17.


   
    Survival and alteration of the plasmid-containing microorganism Escherichia coli Z905/pPHL7 introduced into manmade closed aquatic microcosms [Text] / A. N. Boyandin [et al.] ; ed. M Nelson [et al.] // SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P. 1763-1768, DOI 10.1016/S0273-1177(03)00118-2. - Cited References: 9 . - ISBN 0273-1177
РУБ Engineering, Aerospace + Astronomy & Astrophysics + Ecology + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences
Рубрики:
MARKER
   SOIL

Аннотация: It has been demonstrated that the transgenic microorganism Escherichia coli Z905/pPHL7 (Ap(r)Lux(+)) can exist for a long time at an elevated concentration of mineral salts. The microorganism was introduced into microcosms with sterile brackish water (salinity variable from 21 to 22 g l(-1)) taken from Lake Shira (Khakasia, Russia). The survival of the microorganism was estimated both by measuring the growth of the colonies on solid nutrient media and by the bioluminescence exhibited by the transgenic strain in samples from the microcosms and in the enrichment culture with the added selective factor - ampicillin (50 mug/ml). In the enrichment culture, the bioluminescent signal was registered through the 160-day experiment. It has been shown that in the closed microcosms with brackish water the E. coli strain becomes heterogeneous in its ampicillin resistance. The wpopulations of the transgenic strain were mainly represented by isolates able to persist in the medium containing 50 mug/ml, but there were also the cells (about 10%) with the threshold of ampicillin resistance not more than 0.05 mug/ml. Thus, it was shown that in the microcosms with brackish water and in the absence of the selective factor the transgenic strain survives and retains the recombinant plasmid. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Lobova, T.I.; Popova, L.Y.; Pechurkin, PNS; Nelson, M \ed.\; Pechurkin, NS \ed.\; Dempster, WF \ed.\; Somova, LA \ed.\; Somo, , LA \ed.\

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18.


   
    Biofilm formation by bacterial associations under various salinities and copper ion stress [Text] / O. A. Mogilnaya [et al.] // Biofouling. - 2005. - Vol. 21, Is. 05.06.2013. - P. 247-255, DOI 10.1080/08924010500445848. - Cited References: 24 . - ISSN 0892-7014
РУБ Biotechnology & Applied Microbiology + Marine & Freshwater Biology
Рубрики:
HEAVY-METAL RESISTANCE
   BACILLUS-SUBTILIS

   PROTEIN

   RISK

Кл.слова (ненормированные):
binary community -- surface films -- adhesion -- copper -- stress
Аннотация: The study addresses the effect of abiotic (medium salinity and copper ions) and biotic (interactions between populations) factors on the formation of structured communities by binary associations consisting of halotolerant bacteria (Alcaligenes sp. 1-1 or Acinetobacter sp. 1-19) and a wild-type B. subtilis 2335 strain or a transgenic strain. The results showed that 250 mg l(-1) of copper ions inhibit formation of biofilms by monocultures of the tested strains. Binary associations of the strains were more resistant to high concentrations (250 mg l(-1)) of copper ions. At the lowest NaCl concentration (0.05% and 2.5%) and in the presence of copper ions, bacilli seemed to help halotolerant bacteria survive. Under increased salinity and in the presence of copper ions, structured communities developed due to halotolerant bacteria. Coexistence under stressful conditions was beneficial for the both groups of bacteria.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
TORINS Co, Krasnoyarsk, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Mogilnaya, O.A.; Lobova, T.I.; Kargatova, T.V.; Popova, L.Y.

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19.


   
    Bacterial Communities of Three Saline Meromictic Lakes in Central Asia [Text] / B. Baatar [et al.] // PLoS One. - 2016. - Vol. 11, Is. 3. - Ст. e0150847, DOI 10.1371/journal.pone.0150847. - Cited References:65. - This study was supported by the thematic project funding of Taiwan-Russian Joint Project (NSC99- 2923-B-001-001-MY3 and NSC 102-2923-B-001-004) and Taiwan-Mongolian Joint Project (NSC101-2923-B-001-003-MY3) from the National Sciences Council of Taiwan and Biodiversity Research Center, Academia Sinica, Taiwan and the Russian Foundation for Basic Research, Grant No. 14-04-01060-a.; We thank our Russian and Mongolian colleagues in the Institute of Biophysics SB RAS and National University of Mongolia for assistance with providing samples and hydro-parameter data. This study was supported by the thematic project funding of Taiwan-Russian Joint Project (NSC99-2923-B-001-001-MY3 and NSC 102-2923-B-001-004) and Taiwan-Mongolian Joint Project (NSC101-2923-B-001-003-MY3) from the National Sciences Council of Taiwan and Biodiversity Research Center, Academia Sinica, Taiwan and the Russian Foundation for Basic Research, Grant No. 14-04-01060-a. . - ISSN 1932-6203
РУБ Multidisciplinary Sciences
Рубрики:
PURPLE SULFUR BACTERIA
   REAL-TIME PCR

   MICROBIAL COMMUNITIES

Аннотация: Meromictic lakes located in landlocked steppes of central Asia (similar to 2500 km inland) have unique geophysiochemical characteristics compared to other meromictic lakes. To characterize their bacteria and elucidate relationships between those bacteria and surrounding environments, water samples were collected from three saline meromictic lakes (Lakes Shira, Shunet and Oigon) in the border between Siberia and the West Mongolia, near the center of Asia. Based on in-depth tag pyrosequencing, bacterial communities were highly variable and dissimilar among lakes and between oxic and anoxic layers within individual lakes. Proteobacteria, Bacteroidetes, Cyanobacteria, Actinobacteria and Firmicutes were the most abundant phyla, whereas three genera of purple sulfur bacteria (a novel genus, Thiocapsa and Halochromatium) were predominant bacterial components in the anoxic layer of Lake Shira (similar to 20.6% of relative abundance), Lake Shunet (similar to 27.1%) and Lake Oigon (similar to 9.25%), respectively. However, few known green sulfur bacteria were detected. Notably, 3.94% of all sequencing reads were classified into 19 candidate divisions, which was especially high (23.12%) in the anoxic layer of Lake Shunet. Furthermore, several hydro-parameters (temperature, pH, dissolved oxygen, H2S and salinity) were associated (P0.05) with variations in dominant bacterial groups. In conclusion, based on highly variable bacterial composition in water layers or lakes, we inferred that the meromictic ecosystem was characterized by high diversity and heterogenous niches.

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Scopus
Держатели документа:
Acad Sinica, Taiwan Int Grad Program, Mol & Biol Agr Sci Program, Taipei 115, Taiwan.
Acad Sinica, Biodivers Res Ctr, Taipei 115, Taiwan.
Natl Chung Hsing Univ, Grad Inst Biotechnol, Taichung 40227, Taiwan.
SB RAS, Inst Biophys, Krasnoyarsk 660036, Russia.
Natl Pingtung Univ Sci & Technol, Pingtung 91201, Taiwan.
Natl Univ Mongolia, Sch Art & Sci, Ulaanbaatar 14201, Mongol Peo Rep.
Natl Chung Hsing Univ, Ctr Biotechnol, Taichung 40227, Taiwan.

Доп.точки доступа:
Baatar, Bayanmunkh; Chiang, Pei-Wen; Rogozin, Denis Yu; Wu, Yu-Ting; Tseng, Ching-Hung; Yang, Cheng-Yu; Chiu, Hsiu-Hui; Oyuntsetseg, Bolormaa; Degermendzhy, Andrey G.; Tang, Sen-Lin; National Sciences Council of Taiwan [NSC99- 2923-B-001-001-MY3, NSC 102-2923-B-001-004, NSC101-2923-B-001-003-MY3, NSC99-2923-B-001-001-MY3]; Biodiversity Research Center, Academia Sinica, Taiwan; Russian Foundation for Basic Research [14-04-01060-a]

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20.


   
    Effects of PAR intensity and NaCl concentration on growth of Salicornia europaea plants as relevant to artificial ecological systems / N. A. Tikhomirova [et al.] // Russ. J. Plant Physiol. - 2016. - Vol. 63, Is. 4. - P474-482, DOI 10.1134/S1021443716040154 . - ISSN 1021-4437
Кл.слова (ненормированные):
artificial ecosystems -- CO2 exchange -- macroelements -- MDA -- NaCl salinity -- PAR intensity -- photosynthetic pigments -- productivity -- Salicornia europaea
Аннотация: Effects of variable levels of photosynthetically active radiation (PAR) and NaCl concentrations, typical of closed ecological life support systems, on growth of Salicornia europaea L. plants, CO2 exchange, mineral composition, and the content of malondialdehyde (MDA) and photosynthetic pigments were investigated. The plants were grown for 25 days at different salinities of nutrient Knop solution (171, 342, and 513 mM NaCl) under two PAR levels (690 and 1150 ?mol/(m2 s)). At PAR of 690 ?mol/(m2 s), the plant productivity did not show significant changes at increasing salinities; at 1150 ?mol/(m2 s), the maximal productivity was observed at NaCl concentrations of 171 and 342 mM. The increase in NaCl concentration from 171 to 513 mM in the nutrient solution led to a substantial increase in the relative Na content in aboveground organs at PAR level of 1150 ?mol/(m2 s). The MDA content in aboveground organs by the end of the growth period was independent of PAR intensity. The content of photosynthetic pigments in the assimilatory tissue decreased with the increase in salinity from 342 to 513 mM NaCl at PAR level of 1150 ?mol/(m2 s) but not at the lower irradiance. The combination of 1150 ?mol/(m2 s) PAR intensity with the salinity as high as 342 mM NaCl was found to be the most effective for optimal productivity of S. europaea plants. © 2016, Pleiades Publishing, Ltd.

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Смотреть статью,
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Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Tikhomirova, N. A.; Ushakova, S. A.; Shklavtsova, E. S.; Anishchenko, O. V.; Mikheeva, Y. A.; Tikhomirov, A. A.

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