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First data on the uranium content in water of the Yenisei River basin in the area affected by the operation of Rosatom plants / A. Y. Bolsunovskii [et al.] // Doklady Earth Sciences. - 2011. - Vol. 439, Is. 1. - P1010-1015, DOI 10.1134/S1028334X11070208 . - ISSN 1028-334X
Кл.слова (ненормированные):
Inorganic chemistry -- Isotope ratio -- Isotopic composition -- Long term monitoring -- River basins -- Russian Academy of Sciences -- Uranium contents -- Uranium isotopes -- Water samples -- Effluents -- Inorganic compounds -- Isotopes -- Mass spectrometry -- Nuclear fuels -- Rivers -- Uranium -- Watersheds -- Water content -- effluent -- environmental monitoring -- isotopic composition -- isotopic ratio -- mass spectrometry -- tributary -- uranium isotope -- Russian Federation -- Yenisei River
Аннотация: This study is devoted to investigating the content of uranium isotopes in water of the Yenisei River and its tributaries within the territories affected by the operation of Rosatom plants (mining chemical combine, and electrochemical plant). Long-term monitoring of the 238U content by mass spectrometry carried out in two institutes of the Siberian Branch of the Russian Academy of Sciences first revealed the multiple excess of 238U over the background content in different areas of the Yenisei River basin, such as the region of the Yenisei River near the effluents of the mining and chemical combine (MCC), and the territories of the Bol'shaya Tel' and Kan rivers. In these regions, the 238U content in water reaches 2.1-4.0 ?g/l, which exceeds its content upstream from the MCC (0.3-0.6 ?g/l) by almost an order of magnitude. The studies of the isotopic composition of uranium in water samples, which were carried out at the Institute of Inorganic Chemistry, Siberian Branch, Russian Academy of Sciences, showed the presence of a technogenic isotope of uranium 236U in the samples from the Bolshaya Tel' River and revealed the deviation of the isotope ratio 238U/ 235U (167 В± 3 and 177 В± 3) from the equilibrium natural ratio ( 238U/ 235U = 138). These facts attest to the technogenic origin of part of the uranium in water of the Bol'shaya Tel' River connected with the activity of MCC. The excess uranium content in the Kan River requires additional studies to ascertain the fraction of uranium of technogenic origin connected with the activity of the electrochemical plant (ECP) (Fig. 1, Table 4). В© 2011 Pleiades Publishing, Ltd.

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Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Institute of Chemistry and Chemical Engineering, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Nikolaev Institute of Inorganic Chemistry, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovskii, A.Y.; Zhizhaev, A.M.; Saprykin, A.I.; Degermendzhi, A.G.; Rubailo, A.I.

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Effect of humic acid on absorption-release processes in the bottom sediments-Yenisei river water system as studied by dual-column ion chromatography and ?-ray spectrometry / L. G. Bondareva, O. P. Kalyakina, A. Ya. Bolsunovskii // Journal of Analytical Chemistry. - 2006. - Vol. 61, Is. 4. - P354-358, DOI 10.1134/S1061934806040101 . - ISSN 1061-9348
Кл.слова (ненормированные):
Absorption -- Chromatographic analysis -- Complexation -- Gamma ray spectrometers -- Radioisotopes -- ?-ray spectrometry -- Absorption-release processes -- Dual-column ion chromatography -- Humic acid -- Organic acids
Аннотация: The effect of humic acid on absorption-release processes in the bottom sediments-Yenisei river water system was studied by dual-column ion chromatography and ?-ray spectrometry. With the use of ion chromatography, it was found that processes related to the absorption of SO 42- and Cl- anions by a solid phase with the release of NO 3- , PO 43- , and F- to a liquid phase competed in the test systems as the concentration of water-soluble organic carbon (WSOC) was increased. Only the test anions were released in the systems without the introduction of an additional amount of WSOC as humic acid. With the use of ?-ray spectrometry, it was found that the release of 60Co, 152Eu, and 241Am radionuclides to the liquid phase in the systems with added humic acid began much earlier than in the system without the addition of humic acid. In this case, the amount of released radionuclides was greater than the amount of radioisotopes released in the system without the addition of humic acid: ?25% 241Am, ?3% 152Eu, and ?0.8% 60Co in the system with added humic acid or 0.8% 152Eu and <0.1% 60Co in the system without the addition of humic acid. The 241Am radionuclide was not determined in the system without the addition of humic acid. An increase in the concentration of WSOC in the experimental system of bottom sediments-Yenisei river water initiated the release of 60Co, 152Eu, and 241Am anthropogenic radionuclides from bottom sediments because of the formation of soluble complexes capable of migration. An increase in the concentration of WSOC had almost no effect on the release of 40K and 137Cs radionuclides. В© Pleiades Publishing, Inc., 2006.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Faculty of Chemistry, Krasnoyarsk State University, Svobodnyi pr. 79, Krasnoyarsk, 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bondareva, L.G.; Kalyakina, O.P.; Bolsunovskii, A.Ya.

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Evidence of the radioactive fallout in the center of asia (russia) following the fukushima nuclear accident / A. Bolsunovsky, D. Dementyev // Journal of Environmental Radioactivity. - 2011. - Vol. 102, Is. 11. - P1062-1064, DOI 10.1016/j.jenvrad.2011.06.007 . - ISSN 0265-931X
Кл.слова (ненормированные):
Environmental samples -- Fission product radionuclides -- Fukushima nuclear accident -- Radioactive fallout -- Russia -- Chernobyl accident -- Environmental sample -- Fallout radionuclides -- Global effects -- High velocity -- Nuclear accidents -- Radioactive contamination -- Russia -- Water samples -- Cesium -- Fallout -- Fission products -- Radioactivity -- Radioisotopes -- Nuclear reactor accidents -- cesium 137 -- iodine 131 -- rain -- atmospheric pollution -- cesium isotope -- fallout -- nuclear accident -- radioactive pollution -- radionuclide -- article -- environmental exposure -- gamma spectrometry -- nuclear accident -- radioactive contamination -- radioactive waste -- water analysis -- water contamination -- Cesium Radioisotopes -- Cities -- Humans -- Iodine Radioisotopes -- Japan -- Radiation Dosage -- Radiation Monitoring -- Radioactive Fallout -- Radioactive Hazard Release -- Radioactive Pollutants -- Risk Assessment -- Russia -- Time Factors -- Fukushima -- Honshu -- Japan -- Krasnoyarsk [Russian Federation] -- Russian Federation -- Tohoku
Аннотация: It was recently reported that radioactive fallout due to the Fukushima Nuclear Accident was detected in environmental samples collected in the USA and Greece, which are very far away from Japan. In April-May 2011, fallout radionuclides ( 134Cs, 137Cs, 131I) released in the Fukushima Nuclear Accident were detected in environmental samples at the city of Krasnoyarsk (Russia), situated in the center of Asia. Similar maximum levels of 131I and 137Cs/ 134Cs and 131I/ 137Cs ratios in water samples collected in Russia and Greece suggest the high-velocity movement of the radioactive contamination from the Fukushima Nuclear Accident and the global effects of this accident, similar to those caused by the Chernobyl accident. В© 2011 Elsevier Ltd.

Scopus
Держатели документа:
Radioecology Laboratory, Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Akademgorodok, 50-50, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.; Dementyev, D.

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Chemical fractionation of radionuclides and stable elements in aquatic plants of the yenisei river / A. Bolsunovsky // Environmental Science and Technology. - 2011. - Vol. 45, Is. 17. - P7143-7150, DOI 10.1021/es2008853 . - ISSN 0013-936X
Кл.слова (ненормированные):
Aquatic plants -- Artificial radionuclides -- Cell structure -- Chemical Fractionation -- Distribution patterns -- Nuclear plant -- Plant biomass -- Sequential extraction scheme -- Stable elements -- Submerged plants -- Transuranic elements -- Americium -- Biomass -- Neptunium -- River pollution -- Rivers -- Radioisotopes -- americium -- element -- neptunium -- radioisotope -- bioaccumulation -- chemical binding -- gamma ray spectrometry -- isotopic fractionation -- monocotyledon -- phytomass -- radionuclide -- river pollution -- submerged vegetation -- aquatic species -- article -- Batrachium kauffmanii -- biomass -- cell structure -- controlled study -- extraction -- Fontinalis antipyretica -- fractionation -- gamma spectrometry -- higher plant -- nonhuman -- plant growth -- Potamogeton lucens -- Potamogetonaceae -- river -- Russian Federation -- Aquatic Organisms -- Biomass -- Chemical Fractionation -- China -- Industrial Waste -- Plants -- Radiation Monitoring -- Radioisotopes -- Rivers -- Water Pollutants, Radioactive -- Russian Federation -- Yenisei River -- Batrachium -- Fontinalis antipyretica -- Potamogeton lucens
Аннотация: The Yenisei River is contaminated with artificial radionuclides released by one of the Russian nuclear plants. The aquatic plants growing in the radioactively contaminated parts of the river contain artificial radionuclides. The aim of the study was to investigate accumulation of artificial radionuclides and stable elements by submerged plants of the Yenisei River and estimate the strength of their binding to plant biomass by using a new sequential extraction scheme. The aquatic plants sampled were: Potamogeton lucens, Fontinalis antipyretica, and Batrachium kauffmanii. Gamma-spectrometric analysis of the samples of aquatic plants has revealed more than 20 radionuclides. We also investigated the chemical fractionation of radionuclides and stable elements in the biomass and rated radionuclides and stable elements based on their distribution in biomass. The greatest number of radionuclides strongly bound to biomass cell structures was found for Potamogeton lucens and the smallest for Batrachium kauffmanii. For Fontinalis antipyretica, the number of distribution patterns that were similar for both radioactive isotopes and their stable counterparts was greater than for the other studied species. The transuranic elements 239Np and 241Am were found in the intracellular fraction of the biomass, and this suggested their active accumulation by the plants. В© 2011 American Chemical Society.

Scopus
Держатели документа:
Institute of Biophysics SB, Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.

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Microdistribution of 241Am in structures of submerged macrophyte Elodea canadensis growing in the Yenisei River / L. Bondareva [et al.] // Journal of Environmental Radioactivity. - 2010. - Vol. 101, Is. 1. - P16-21, DOI 10.1016/j.jenvrad.2009.08.003 . - ISSN 0265-931X
Кл.слова (ненормированные):
Alpha-track analysis -- Americium -- Elodea canadensis -- Liquid-scintillation spectrometry -- Microdistribution -- Alpha-track analysis -- Elodea canadensis -- Microdistribution -- Scintillation spectrometry -- Track analysis -- Liquids -- Luminescence -- Radioisotopes -- Scintillation -- Spectrometry -- Spectroscopy -- Americium -- americium 241 -- americium -- americium -- bioaccumulation -- experimental study -- leaf -- macrophyte -- mass spectrometry -- morphology -- radionuclide -- spatial distribution -- stem -- submerged vegetation -- tracking -- aquatic flora -- article -- bioaccumulation -- concentration (parameters) -- elodea canadensis -- environmental radioactivity -- isotope analysis -- isotope tracing -- leaf lamina -- leaf surface -- macrophyte -- nonhuman -- plant cell -- plant morphology -- plant stem -- radioisotope distribution -- river -- chemistry -- Hydrocharitaceae -- metabolism -- plant -- plant leaf -- radiation monitoring -- river -- water pollutant -- Eurasia -- Russian Federation -- Yenisei River -- Elodea canadensis -- Americium -- Hydrocharitaceae -- Plant Leaves -- Plant Shoots -- Plant Stems -- Radiation Monitoring -- Rivers -- Water Pollutants, Radioactive
Аннотация: A submerged macrophyte of the Yenisei River, Elodea canadensis, was used to study the microdistribution of the artificial radionuclide 241Am among different components of the plant. The total amount of 241Am added to the experimental system was 1850 В± 31 Bq/L. The total amount of 241Am accumulated by the plants was 182 Bq per sample, or 758,333 В± 385 Bq/kg dry mass. It has been found that the major portion of 241Am accumulated by E. canadensis, up to 85%, was bound to solid components of the cells. It is observed that the microdistribution of 241Am within different components of the submerged plant E. canadensis was not uniform. 241Am distribution vary depending on the age of the leaf blades, the state of the cells and morphological features of the plant stem. В© 2009 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, 50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Chemistry Department, Lomonosov Moscow State University, Leninskie Gory, Moscow 119991, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bondareva, L.; Vlasova, I.; Mogilnaya, O.; Bolsunovsky, A.; Kalmykov, S.

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Accumulation of artificial radionuclides by the Yenisei river aquatic plants in the area affected by the activity of the Mining-and-Chemical Combine / A. Ya. Bolsunovskij [и др.] // Radiatsionnaya Biologiya. Radioekologiya. - 2002. - Vol. 42, Is. 2. - С. 194-199 . - ISSN 0869-8031
Кл.слова (ненормированные):
Contamination -- Ecosystems -- Environmental impact -- Gamma ray spectrometers -- Radioisotopes -- River pollution -- Radioecological concentration -- Radioisotope accumulation -- The Yenisei river -- Ecology -- plutonium -- radioisotope -- strontium -- article -- chemical industry -- chemistry -- comparative study -- gamma spectrometry -- industrial waste -- mining -- nuclear reactor -- plant -- radiochemistry -- Russian Federation -- water pollutant -- Chemical Industry -- Industrial Waste -- Mining -- Nuclear Reactors -- Plants -- Plutonium -- Radiochemistry -- Radioisotopes -- Siberia -- Spectrometry, Gamma -- Strontium Radioisotopes -- Water Pollutants, Radioactive
Аннотация: The aim of the paper is to investigate accumulation of artificial radionuclides by the Yenisei river aquatic plants collected in the area affected by the activity of the Mining-and-Chemical Combine (Zheleznogorsk) from 1997 to 2000. The samples of aquatic plants were of four species: Potamogeton lucens, Fontinalis antipyretica, Elodea canadensis, and Ceratophyllum demersum. The gamma-spectrometric analysis of the samples of aquatic plants for artificial radionuclides revealed a wide spectrum of radionuclides. Radionuclides of activation origin were found in the aquatic plants taken both near the Combine and 200 km down of it. The radiochemical analysis of aquatic plants revealed strontium and plutonium isotopes. Among the aquatic plants, the highest concentration factors for the principal radionuclides were recorded in Fontinalis antipyretica (water moss).

Scopus
Держатели документа:
Inst. of Biophysics, Siberian Branch, RAS, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovskij, A.Ya.; Ermakov, A.I.; Burger, M.; Degermendzhy, A.G.; Sobolev, A.I.

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Fatty acid composition of Wautersia eutropha lipids under conditions of active polyhydroxyalkanoates synthesis / G. S. Kalacheva, T. G. Volova // Microbiology. - 2007. - Vol. 76, Is. 5. - P535-540, DOI 10.1134/S0026261707050049 . - ISSN 0026-2617
Кл.слова (ненормированные):
Fatty acids -- Polyhydroxyalkanoates -- Wautersia eutropha -- Cupriavidus necator
Аннотация: The fatty acid composition of the lipids of a Wautersia eutropha polyhydroxyalkanoate-producing strain was studied by chromato-mass spectrometry. A total of 27 fatty acids were identified; their distribution in the cell fractions was determined. In the cytoplasmic membrane, palmitic, palmitoleic, and cis-vaccenic acids were the major components. Long-chain ?-hydroxy acids and myristic acids (components of the lipopolysaccharides of the cell envelope) predominated in the fraction of strongly bound lipids. When the polymer was actively synthesized, the content of cyclopropane acids in the easily extracted lipids increased and the content of the corresponding monoenoic acids decreased. The strongly bound lipids had a high content of long-chain ?-hydroxy acids (more than 50% of the total fatty acids). These results made it possible to determine the source of polyhydroxyalkanoate (PHA) contamination and to choose the strategy for their purification. В© 2007 Pleiades Publishing, Ltd.

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Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kalacheva, G.S.; Volova, T.G.

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Production of purified polyhydroxyalkanoates (PHAs) for applications in contact with blood / V. I. Sevastianov [et al.] // Journal of Biomaterials Science, Polymer Edition. - 2003. - Vol. 14, Is. 10. - P1029-1042, DOI 10.1163/156856203769231547 . - ISSN 0920-5063
Кл.слова (ненормированные):
?-hydroxy acids -- Endotoxins -- Hemocompatibility -- Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) -- Polyhydroxyalkanoates (PHAs) -- Polyhydroxybutyrate (PHB) -- bacterium lipopolysaccharide -- carbon -- complement -- copolymer -- hydroxyacid -- long chain fatty acid -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- valeric acid derivative -- adult -- article -- biofilm -- biotechnology -- blood analysis -- blood clotting -- blood compatibility -- cell function -- chemical analysis -- chemical composition -- complement activation -- concentration (parameters) -- controlled study -- gas chromatography -- hemostasis -- human -- human cell -- mass spectrometry -- micromorphology -- nonhuman -- priority journal -- purification -- quantitative analysis -- sampling -- synthesis -- thrombocyte adhesion -- Wautersia eutropha -- Biocompatible Materials -- Blood -- Blood Coagulation Tests -- Chromatography, Gas -- Complement Activation -- Cupriavidus necator -- Fatty Acids -- Humans -- Platelet Adhesiveness -- Polyesters -- Surface Properties
Аннотация: Samples of olyhydroxyalkanoates (PHAs), polyhydroxybutyrate (PHB) and copolymers poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) with 4 and 18 mol% hydroxyvalerate, synthesized by the bacteria Ralstonia eutropha B5786, were investigated. PHA films in contact with blood did not activate the hemostasis system at the level of cell response, but they did activate the coagulation system and the complement reaction. To detect biologically-active components in the PHAs, a detailed analysis of the composition of the polymers was conducted. Gas chromatography-mass spectrometry revealed long-chain fatty acids (FAs) in the tested PHAs. Their total concentration in the polymer ranged from tenths of mol% to 2-3 mol%, depending on the purification method. C16:0 constituted the largest proportion, up to 70%. Of the long-chain hydroxy acids, only ?-OH-C14:0 was detected and it did not exceed 0.06 mol%. The analysis of the hemocompatibility properties of the PHAs purified by a specialized procedure, including the quantitative and morphological estimation of platelets adherent to the surface of polymer films, the plasma recalcification time and complement activation studies, indicated that PHB and PHBV can be used in contact with blood. It has been found out that the lipopolysaccharides of bacteria producing PHAs, which contain mostly long-chain hydroxy acids, can be the factor activating the hemostasis systems. Thus, the technology of PHA purification must satisfy rather stringent specific requirements.

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Держатели документа:
Inst. of Transplantol. Artif. Organs, Russian Ministry of Health, Shchukinskaya 1, 123182 Moscow, Russian Federation
Inst. of Biophys. of Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sevastianov, V.I.; Perova, N.V.; Shishatskaya, E.I.; Kalacheva, G.S.; Volova, T.G.

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The autotrophic synthesis of polyhydroxyalkanoate by alcaligenes eutrophus in the presence of carbon monoxide / T. G. Volova, G. S. Kalacheva, O. V. Altukhova // Mikrobiologiya. - 2001. - Vol. 70, Is. 6. - С. 745-752 . - ISSN 0026-3656
Кл.слова (ненормированные):
CO inhibition -- Hydrogen-oxidizing bacteria -- Polyhydroxyalkanoates (PHAs) -- Synthesis -- alkane -- carbon monoxide -- fatty acid -- hydroxyacid -- lipid -- nitrogen -- polyester -- article -- chemistry -- culture medium -- drug antagonism -- growth, development and aging -- mass spectrometry -- metabolism -- Wautersia eutropha -- Alkanes -- Carbon Monoxide -- Culture Media -- Cupriavidus necator -- Fatty Acids -- Hydroxy Acids -- Lipids -- Mass Spectrometry -- Nitrogen -- Polyesters
Аннотация: The CO-resistant strain B5786 of the hydrogen-oxidizing bacterium Alcaligenes eutrophus was found to be able to synthesize polyhydroxyalkanoates (PHAs) under the conditions of growth limitation by nitrogen deficiency (the factor that promotes PHA synthesis) and growth inhibition by carbon monoxide. The gas mixtures that contained from 5 to 20 vol % CO did not inhibit the key enzymes of PHA synthesis-?-ketothiolase, acetoacetyl-CoA reductase, hydroxybutyrate dehydrogenase, and PHA synthase. In the presence of CO, cells accumulated up to 70-75 wt % PHA (with respect to the dry biomass) without any noticeable increase in the consumption of the gas substrate. Chromatographic-mass spectrometric analysis showed that the PHA synthesized by A. eutrophus is a copolymer containing more than 99 mol % ?-hydroxybutyrate and trace amounts of ?-hydroxyvalerate. The PHA synthesized under the conditions described did not differ from that synthesized by A. eutrophus cells from electrolytic hydrogen.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Altukhova, O.V.

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10.

Experimental investigations of 241Am accumulation by macrophytes of the Yenisei River / A. Ya. Bolsunovskij, T. A. Zotina // Radiatsionnaya Biologiya. Radioekologiya. - 2002. - Vol. 42, Is. 2. - С. 200-203 . - ISSN 0869-8031
Кл.слова (ненормированные):
Americium -- Aquaculture -- Biomass -- Ecosystems -- Radioisotopes -- Americium 241 -- Macrophytes -- Radioecological concentration -- The Yenisei river -- River pollution -- americium -- absorption -- article -- biomass -- chemistry -- comparative study -- gamma spectrometry -- metabolism -- plant -- Russian Federation -- time -- water pollutant -- Absorption -- Americium -- Biomass -- Plants -- Siberia -- Spectrometry, Gamma -- Time Factors -- Water Pollutants, Radioactive
Аннотация: Experiments were carried out in which 241Am was added to water samples containing macrophytes of the Yenisei River, and the radionuclide absorption rates and concentration factors were determined for the plants. It has been shown that the water moss (Fontinalis antipyretica) has a higher capacity to accumulate 241Am than the Canadian pondweed (Elodea canadensis) does. The laboratory experiments revealed that the capacity of dead biomass of the Canadian pondweed to accumulate 241Am is twice higher than that of living biomass. In contrast, no significant increase in 241Am accumulation by dead biomass of the water moss has been recorded. The transuranic element 241Am was firmly fixed by the plant biomass and was not released into water in the course of long-duration experiments.

Scopus
Держатели документа:
Inst. of Biophysics, Siberian Branch, RAS, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovskij, A.Ya.; Zotina, T.A.

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11.

Effect of temperature on contents of essential highly unsaturated fatty acids in freshwater zooplankton / M. I. Gladyshev [et al.] // Limnologica. - 2011. - Vol. 41, Is. 4. - P339-347, DOI 10.1016/j.limno.2011.03.001 . - ISSN 0075-9511
Кл.слова (ненормированные):
Cladocerans -- Copepods -- Essential polyunsaturated fatty acids -- Freshwater zooplankton -- Water temperature -- biomass -- climate effect -- community structure -- fatty acid -- freshwater ecosystem -- gas chromatography -- lake ecosystem -- multivariate analysis -- seston -- taxonomy -- temperature effect -- trophic level -- zooplankton -- Animalia -- Copepoda -- Crustacea
Аннотация: In 11 lakes from cold and warm regions we studied the content of highly unsaturated fatty acids (HUFA) in seston (<130?m) and crustacean zooplankton using gas chromatography-mass spectrometry. An increase of temperature correlated with a decrease of HUFA content in zooplankton. A multivariate canonical correlation analyses revealed, that the decrease of HUFA content was related with a decrease of per cent of copepods in zooplankton communities, which are known to have higher HUFA levels in their biomass, than cladocerans. This means that temperature primarily affected the HUFA levels indirectly, via changing of taxonomic structure of zooplankton community, while the homeoviscous adaptation of zooplankton individuals had comparatively lower importance. As found, water temperature was better predictor of HUFA contents of zooplankton, than the fatty acid composition of seston. Thus, it can be predicted, that a probable climate warming will decrease the content of the essential HUFA in freshwater zooplankton with possible negative consequences for animals of higher trophic levels. В© 2011 Elsevier GmbH.

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Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, Svobodny av. 79, Krasnoyarsk 660041, Russian Federation
Scientific and Practical Center of the National Academy of Sciences of Belarus on Bioresources, 27 Akademicheskaya Str., 220072 Minsk, Belarus
Inst. of Biology of Komi Scientific Center of Ural Division of Russian Academy of Sciences, 28 Kommunisticheskaya Str., Syktyvkar 167982, Russian Federation
Kamchatka Research Inst. of Fisheries and Oceanography, Naberezhnaya 18, Petropavlovsk-Kamchatskii 683602, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Semenchenko, V.P.; Dubovskaya, O.P.; Fefilova, E.B.; Makhutova, O.N.; Buseva, Z.F.; Sushchik, N.N.; Razlutskij, V.I.; Lepskaya, E.V.; Baturina, M.A.; Kalachova, G.S.; Kononova, O.N.

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12.

Seasonal dynamics of fatty acids in the lipids of water moss Fontinalis antipyretica from the Yenisei River / G. S. Kalacheva [et al.] // Russian Journal of Plant Physiology. - 2009. - Vol. 56, Is. 6. - P795-807, DOI 10.1134/S1021443709060090 . - ISSN 1021-4437
Кл.слова (ненормированные):
Acetylenic acids -- Fatty acids -- Fontinalis antipyretica -- Seasonal dynamics -- Bryophyta -- Fontinalis antipyretica
Аннотация: Identification of lipid fatty acids (FA) and studying of their seasonal dynamics in water moss Fontinalis antipyretica from the Yenisei River were carried out by means of gas chromatography-mass spectrometry. FA composition of water moss was notable for a relatively low level of saturated acids and predominance of polyunsaturated acids (PUFA) with double bonds (accounting for more than 30% of total FA) and polyunsaturated acids with double and triple bonds (acetylenic acids, accounting for more than 40% of total FA). Among PUFA, ?- and ?-linolenic (18:3?3 and 18:3?6), arachidonic (20:4?6), and eicosapentaenoic (20:5?3) acids prevailed. Relative content of PUFA from ?3-family was the greatest in spring, and the level of PUFA from ?6-group was essentially the same throughout all the seasons. In the biomass of water moss, we identified seven acetylenic acids; among them octadeca-9,12-dien-6-ynoic (6a,9,12-18:3), octadeca-9,12,15-trien-6-ynoic (6a,9,12,15-18:4), and eicosa-11,14-dien-8-ynoic (8a,11,14-20:3) acids were predominant. For the first time, in the lipids of water moss we identified an acetylenic eicosa-11,14,17-trien-8-ynoic acid (8a,11,14,15-20:4). Relative content of acetylenic acids in the total FA was great throughout the entire period of investigation with the peak accumulation in summer. Owing to a steadily high level in the biomass of water moss and the lack of other producers of these acids in the ecosystem, acetylenic FA are highly specific biochemical markers useful for the investigation of trophic interactions between higher aquatic plants and zoobenthos. В© Pleiades Publishing, Ltd., 2009.

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Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kalacheva, G.S.; Sushchik, N.N.; Gladyshev, M.I.; Makhutova, O.N.

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13.

Content of essential polyunsaturated fatty acids in three canned fish species / M. I. Gladyshev [et al.] // International Journal of Food Sciences and Nutrition. - 2009. - Vol. 60, Is. 3. - P224-230, DOI 10.1080/09637480701664761 . - ISSN 0963-7486
Кл.слова (ненормированные):
Canned fish -- Essential polyunsaturated fatty acids -- Herring -- Saury -- Sprat -- docosahexaenoic acid -- essential fatty acid -- icosapentaenoic acid -- linoleic acid -- linolenic acid -- oleic acid -- polyunsaturated fatty acid -- article -- Baltic sprat -- canned food -- controlled study -- fatty acid analysis -- fish -- food analysis -- nonhuman -- Pacific herring -- Pacific saury -- Russian Federation -- species difference -- Animals -- Fatty Acids, Unsaturated -- Fish Products -- Fishes -- Food Handling -- Food, Preserved -- Gas Chromatography-Mass Spectrometry -- Russia -- Clupea harengus -- Clupea pallasi -- Clupeidae -- Cololabis saira -- Sprattus sprattus -- Sprattus sprattus balticus
Аннотация: Three canned fish species - Pacific saury (Cololabis saira), Pacific herring (Clupea harengus) and Baltic sprat (Sprattus sprattus) - most common and popular in Russia, were analyzed for fatty acids. Special attention was paid to long-chain essential polyunsaturated fatty acids: eicosapentaenoic acid (20:5?3) and docosahexaenoic acid (22:6?3). Sums of eicosapentaenoic acid and docosahexaenoic acid in saury, herring and sprat were, on average, 2.42, 1.80 and 1.43 g/100 g product, respectively. Contents of these essential acids in all the canned fish species were found to be very high compared with many other fish reported in the available literature. All the canned fish appeared to be highly valuable products for human nutrition concerning the content of eicosapentaenoic and docosahexaenoic acids.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Sushchik, N.N.; Makhutova, O.N.; Kalachova, G.S.

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14.

Volatile metabolites of higher plant crops as a photosynthesizing life support system component under temperature stress at different light intensities / I. I. Gitelson [et al.] // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1781-1786, DOI 10.1016/S0273-1177(03)00121-2 . - ISSN 0273-1177
Кл.слова (ненормированные):
Composition -- Crops -- Heat resistance -- Metabolites -- Photosynthesis -- Volatile metabolites -- Space research -- biosphere -- article -- comparative study -- gas -- growth, development and aging -- heat -- indoor air pollution -- light -- metabolism -- microclimate -- photon -- photosynthesis -- physiology -- radiation exposure -- volatilization -- wheat -- Air Pollution, Indoor -- Environment, Controlled -- Gases -- Heat -- Life Support Systems -- Light -- Photons -- Photosynthesis -- Triticum -- Volatilization
Аннотация: The effect of elevated temperatures of 35 and 45В°C (at the intensities of photosynthetically active radiation 322, 690 and 1104 ?mol-m-2-s-1) on the photosynthesis, respiration, and qualitative and quantitative composition of the volatiles emitted by wheat (Triticum aestuvi L., cultivar 232) crops was investigated in growth chambers. Identification and quantification of more than 20 volatile compounds (terpenoids - ?-pinene, ?3 carene, limonene, benzene, ?-and transcaryophyllene, ?- and ?-terpinene, their derivatives, aromatic hydrocarbons, etc.) were conducted by gas chromatograph/mass spectrometry. Under light intensity of 1104 ?mol-m-2-s-1, heat resistance of photosynthesis and respiration increased at 35В°C and decreased at 45В°C. The action of elevated temperatures brought about variations in the rate and direction of the synthesis of volatile metabolites. The emission of volatile compounds was the greatest under a reduced irradiation of 322 ?mol-m-2-s-1 and the smallest under 1104 ?mol-m-2-s-1 at 35В°C. During the repair period, the contents and proportions of volatile compounds were different from their initial values, too. The degree of disruption and the following recovery of the functional state depended on the light intensity during the exposure to elevated temperatures. The investigation of the atmosphere of the growth chamber without plants has revaled the substances that were definitely technogenic in origin: tetramethylurea, dimethylsulfide, dibutylsulfide, dibutylphthalate, and a number of components of furan and silane nature. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, I.I.; Tikhomirov, A.A.; Parshina, O.V.; Ushakova, S.A.; Kalacheva, G.S.

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15.

An ontogenetic approach to the assessment of plant resistance to stress factors based on the method of chlorophyll fluorescence induction. / T. V. Nesterenko, A. A. Tikhomirov // Doklady. Biochemistry and biophysics. - 2003. - Vol. 388. - P4-7 . - ISSN 1607-6729
Кл.слова (ненормированные):
chlorophyll -- adaptation -- aging -- article -- comparative study -- cucumber -- light -- metabolism -- methodology -- photostimulation -- photosynthesis -- physiology -- plant leaf -- radiation dose -- radiation exposure -- spectrofluorometry -- Adaptation, Physiological -- Aging -- Chlorophyll -- Cucumis sativus -- Light -- Photic Stimulation -- Photosynthetic Reaction Center Complex Proteins -- Plant Leaves -- Radiation Dosage -- Spectrometry, Fluorescence

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Nesterenko, T.V.; Tikhomirov, A.A.

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16.

Assessment of plant leaf response to long-term exposure at high temperature using the method of thermoinduced chlorophyll fluorescence. / T. V. Nesterenko [et al.] // Doklady. Biochemistry and biophysics. - 2001. - Vol. 378. - P165-169 . - ISSN 1607-6729
Кл.слова (ненормированные):
chlorophyll -- pigment -- article -- biosynthesis -- chemistry -- light -- metabolism -- plant leaf -- plant physiology -- radiation exposure -- spectrofluorometry -- temperature -- thermodynamics -- time -- Chlorophyll -- Light -- Pigments, Biological -- Plant Leaves -- Plant Physiology -- Spectrometry, Fluorescence -- Temperature -- Thermodynamics -- Time Factors

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Nesterenko, T.V.; Tikhomirov, A.A.; Shikhov, V.N.; Zavorueva, E.N.

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17.

Effect of humic acid on absorption-release processes in the bottom sediments-Yenisei river water system as studied by dual-column ion chromatography and gamma-ray spectrometry [Text] / L. G. Bondareva, O. P. Kalyakina, A. Y. Bolsunovskii // J. Anal. Chem. - 2006. - Vol. 61, Is. 4. - P354-358, DOI 10.1134/S1061934806040101. - Cited References: 11 . - 5. - ISSN 1061-9348

РУБ Chemistry, Analytical

Аннотация: The effect of humic acid on absorption-release processes in the bottom sediments-Yenisei river water system was studied by dual-column ion chromatography and gamma-ray spectrometry. With the use of ion chromatography, it was found that processes related to the absorption of SO42- and Cl- anions by a solid phase with the release of NO3-, PO43-, and F- to a liquid phase competed in the test systems as the concentration of water-soluble organic carbon (WSOC) was increased. Only the test anions were released in the systems without the introduction of an additional amount of WSOC as humic acid. With the use of gamma-ray spectrometry, it was found that the release of Co-60, Eu-152, and Am-241 radionuclides to the liquid phase in the systems with added humic acid began much earlier than in the system without the addition of humic acid. In this case, the amount of released radionuclides was greater than the amount of radioisotopes released in the system without the addition of humic acid: similar to 25% Am-241, similar to 3% Eu-152, and similar to 0.8% Co-60 in the system with added humic acid or 0.8% Eu-152 and 0.1% Co-60 in the system without the addition of humic acid. The Am-241 radionuclide was not determined in the system without the addition of humic acid. An increase in the concentration of WSOC in the experimental system of bottom sediments-Yenisei river water initiated the release of Co-60, Eu-152, and Am-241 anthropogenic radionuclides from bottom sediments because of the formation of soluble complexes capable of migration. An increase in the concentration of WSOC had almost no effect on the release of K-40 and Cs-137 radionuclides.

Держатели документа:
Russian Acad Sci, Siberian Div, Inst Biophys, Krasnoyarsk 660036, Russia
Krasnoyarsk State Univ, Fac Chem, Krasnoyarsk 660041, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bondareva, L.G.; Kalyakina, O.P.; Bolsunovskii, A.Y.

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18.

LC-MS and microscale NMR analysis of luciferin-related compounds from the bioluminescent earthworm Fridericia heliota / S. M. Marques [et al.] // Journal of Photochemistry and Photobiology B: Biology. - 2011. - Vol. 102, Is. 3. - P218-223, DOI 10.1016/j.jphotobiol.2010.12.006 . - ISSN 1011-1344
Кл.слова (ненормированные):
Bioluminescence -- Earthworm -- Fridericia heliota -- Luciferin -- Microscale NMR -- RP-HPLC-MS -- alkene -- alkyl group -- benzothiazole -- carboxylic acid -- hydroxyl group -- luciferin -- pterin -- absorption -- article -- bioluminescence -- earthworm -- Fridericia heliota -- isomer -- molecular weight -- nonhuman -- nuclear magnetic resonance -- priority journal -- reversed phase high performance liquid chromatography -- ultraviolet radiation -- Animals -- Chromatography, High Pressure Liquid -- Chromatography, Reverse-Phase -- Firefly Luciferin -- Luminescent Agents -- Magnetic Resonance Spectroscopy -- Mass Spectrometry -- Oligochaeta -- Fridericia heliota
Аннотация: This paper presents the main results of RP-HPLC-MS and microscale NMR analysis performed on Accompanying similar to Luciferin (AsLn(x)), compounds present in extracts of the bioluminescent earthworm Fridericia heliota that display similarities with Fridericia's luciferin, the substrate of the bioluminescent reaction. Three isomers of AsLn were discovered, AsLn(1), AsLn(2) and AsLn(3), all of which present a molecular weight of 529 Da. Their UV-Vis absorption spectra show maxima at 235 nm for AsLn(1), 238 and 295 nm for AsLn(2) and 241 and 295 nm for AsLn(3). MS n fragmentation patterns suggest the existence of carboxylic acid and hydroxyl moieties, and possibly chemical groups found in other luciferins like pterin or benzothiazole. The major isomer, AsLn(2), presents an aromatic ring and alkene and alkyl moieties. These luciferin-like compounds can be used as models that could give further insights into the structure of this newly discovered luciferin. В© 2010 Elsevier Inc. All rights reserved.

Scopus
Держатели документа:
Department of Chemistry and Biochemistry, Faculty of Sciences, Universidade Do Porto, Rua do Campo Alegre 687, 4169-007 Porto, Portugal
Laboratory of Photobiology, Institute of Biophysics, Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Marques, S.M.; Petushkov, V.N.; Rodionova, N.S.; Esteves Da Silva, J.C.G.

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19.

The yellow bioluminescence bacterium, Vibrio fischeri Y1, contains a bioluminescence active riboflavin protein in addition to the yellow fluorescence FMN protein / V. N. Petushkov, B. G. Gibson, J. Lee // Biochemical and Biophysical Research Communications. - 1995. - Vol. 211, Is. 3. - P774-779, DOI 10.1006/bbrc.1995.1880 . - ISSN 0006-291X
Кл.слова (ненормированные):
riboflavin -- article -- bioluminescence -- fluorescence -- nonhuman -- priority journal -- protein analysis -- protein synthesis -- vibrio -- vibrionaceae -- Bacterial Proteins -- Chromatography, Gel -- Chromatography, Thin Layer -- Flavin Mononucleotide -- Flavoproteins -- Luminescence -- Riboflavin -- Spectrometry, Fluorescence -- Support, U.S. Gov't, P.H.S. -- Vibrio -- Bacteria (microorganisms) -- Photobacterium -- Vibrio -- Vibrio fischeri
Аннотация: The yellow bioluminescence Y1 strain of Vibrio fischeri can produce a 22 kDa protein with either FMN or riboflavin as a bound fluorophore. Both forms are active for shifting the bioluminescence spectral maximum. The fluorescence spectral distribution of the two proteins differs slightly and the in vivo emission appears to be an equal mixture of the two. The bioluminescence activity of the riboflavin Y1 protein contrasts with the inactivity of the related Photobacterium type.

Scopus
Держатели документа:
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, United States
Institute of Biophysics, Academy of Sciences of Russia (Siberian Branch), 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V.N.; Gibson, B.G.; Lee, J.

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20.

AsLn2, a luciferin-related modified tripeptide from the bioluminescent earthworm Fridericia heliota / V. N. Petushkov [et al.] // Tetrahedron Letters. - 2014. - Vol. 55, Is. 2. - P463-465, DOI 10.1016/j.tetlet.2013.11.061 . - ISSN 0040-4039
Кл.слова (ненормированные):
Bioluminescence -- Fridericia heliota -- Luciferin -- Modified peptide
Аннотация: AsLn2, an unusual modified peptide, was isolated from the bioluminescent earthworm Fridericia heliota (Enchytraeidae). Its structure, elucidated by NMR and mass spectrometry, includes residues of tyrosine, CompX (a novel tyrosine modification product, reported in the accompanying paper), and N(omega)-acylated lysine. Chromatography, UV, and 1H NMR data imply a close structural similarity of AsLn2 with F. heliota luciferin. AsLn2 appears to be an intermediate or by-product in F. heliota luciferin biosynthesis. © 2013 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Laboratory of Bioluminescent Biotechnologies, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, pr. Svobodnyi, 79, Krasnoyarsk 660041, Russian Federation
Laboratory of Photobiology, Institute of Biophysics, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russian Federation
Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, Campo Alegre St. 687, Porto 4169-007, Portugal
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V.N.; Dubinnyi, M.A.; Rodionova, N.S.; Nadezhdin, K.D.; Marques, S.M.; Esteves Da Silva, J.C.G.; Shimomura, O.; Yampolsky, I.V.

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