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Biodegradation of polyhydroxyalkanoates (PHAs) in tropical coastal waters and identification of PHA-degrading bacteria / T. G. Volova [et al.] // Polymer Degradation and Stability. - 2010. - Vol. 95, Is. 12. - P2350-2359, DOI 10.1016/j.polymdegradstab.2010.08.023 . - ISSN 0141-3910
Кл.слова (ненормированные):
Biopolymers in marine environment -- Degradation of polymers -- PHA properties -- PHA-degrading microorganisms -- Polyhydroxyalkanoates, PHA -- 16S rRNA -- 3-hydroxybutyric acid -- Amorphous phase -- Bacillus sp -- Biodegradation rate -- Chemical compositions -- Coastal waters -- Degradation rate -- Degrading bacteria -- Degree of crystallinity -- Enterobacter -- Hydroxyvalerate -- Marine environment -- PHA properties -- PHA-degrading microorganisms -- Polydispersity indices -- Polyhydroxyalkanoates -- Polymer chains -- Preparation technique -- Sole carbon source -- South China Sea -- Viet Nam -- Acids -- Bacteriology -- Biodegradable polymers -- Biodegradation -- Biomolecules -- Biopolymers -- Microorganisms -- Organic polymers -- Polydispersity -- Polymer films -- Polymers -- RNA -- Seawater -- Degradation
Аннотация: Biodegradability patterns of two PHAs: a polymer of 3-hydroxybutyric acid (3-PHB) and a copolymer of 3-hydroxybutyric and 3-hydroxyvaleric acids (3-PHB/3-PHV) containing 11 mol% of hydroxyvalerate, were studied in the tropical marine environment, in the South China Sea (Nha Trang, Vietnam). No significant differences have been observed between degradation rates of 3-PHB and 3-PHB/3-PHV specimens; it has been found that under study conditions, biodegradation is rather influenced by the shape of the polymer item and the preparation technique than by the chemical composition of the polymer. Biodegradation rates of polymer films in seawater have been found to be higher than those of compacted pellets. As 3-PHB and 3-PHB/3-PHV are degraded and the specimens lose their mass, molecular weight of both polymers is decreased, i.e. polymer chains get destroyed. The polydispersity index of the PHAs grows significantly. However, the degree of crystallinity of both PHAs remains unchanged, i.e. the amorphous phase and the crystalline one are equally disintegrated. PHA-degrading microorganisms were isolated using the clear-zone technique, by inoculating the isolates onto mineral agar that contained PHA as sole carbon source. Based on the 16S rRNA analysis, the PHA-degrading strains were identified as Enterobacter sp. (four strains), Bacillus sp. and Gracilibacillus sp.В© 2010 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, 79 Svobodny av., Krasnoyarsk 660041, Russian Federation
L.V. Kirensky Institute of Physics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation
A.N. Severtsov Institute of Ecology and Evolution RAS, 33 Leninskij Prospect, Moscow 119071, Russian Federation
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, 8 Lavrentiev Ave., Novosibirsk 630090, Russian Federation
Joint Russian-Vietnam Tropical Research and Test Centre, Coastal Branch, 30 Nguyen Thien Thuat, Nha Trang, Viet Nam : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Boyandin, A.N.; Vasiliev, A.D.; Karpov, V.A.; Prudnikova, S.V.; Mishukova, O.V.; Boyarskikh, U.A.; Filipenko, M.L.; Rudnev, V.P.; Ba Xuan, B.; Vit Dung, V.; Gitelson, I.I.

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Degradation of polyhydroxyalkanoates in eutrophic reservoir / T. G. Volova [et al.] // Polymer Degradation and Stability. - 2007. - Vol. 92, Is. 4. - P580-586, DOI 10.1016/j.polymdegradstab.2007.01.011 . - ISSN 0141-3910
Кл.слова (ненормированные):
16S rDNA -- Degradation -- DGGE -- Polyhydroxyalkanoates -- Reservoir -- Biodegradation -- Climate change -- Ecosystems -- Eutrophication -- Genetic engineering -- Physical chemistry -- Eutrophic reservoir -- Phylogenetic analysis -- Physicochemical properties -- Polyhydroxyalkanoates -- Organic polymers
Аннотация: During the summers of 1999-2001 the dynamics of polyhydroxyalkanoate degradation in a small recreational eutrophic reservoir was studied experimentally. It has been shown that biodegradation of polyhydroxyalkanoates in the environment is determined by the structure and physicochemical properties of the polymer and by local weather conditions, which influence the state of the aquatic ecosystem. Species (clones) of bacteria able to utilize polyhydroxyalkanoates in the reservoir were identified using molecular phylogenetic analysis of 16S rRNA genes. В© 2007 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Siberian Federal University, Svobodny av. 79, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Gladyshev, M.I.; Trusova, M.Y.; Zhila, N.O.

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Results of analysis of spatial and temporal long-term stability of quasistationary areas in the Atlantic Ocean based on multi-satellite measurements (CZCS and SeaWiFS data) / A. Shevyrnogov, G. Vysotskaya, E. Shevyrnogov // Advances in Space Research. - 2004. - Vol. 33, Is. 7. - P1184-1188, DOI 10.1016/S0273-1177(03)00366-1 . - ISSN 0273-1177
Кл.слова (ненормированные):
Chlorophyll -- Hydrological structure -- Seasonal dynamics -- Space-based data -- Chlorophyll -- Data reduction -- Hydrology -- Oceanography -- Satellites -- Quasistationary -- Spatial distribution -- Space research -- chlorophyll -- CZCS -- oceanic circulation -- remote sensing -- SeaWiFS -- spatiotemporal analysis -- Atlantic Ocean
Аннотация: The paper analyzes the stability of areas in the Global Ocean, which are quasistationary with respect to the seasonal dynamics of chlorophyll concentration. Such areas were first discovered based on the CZCS data. They occur almost in all regions of the Global Ocean. They reflect typical seasonal dynamics of phytoplankton, which depends on both large ocean currents and local hydrological instability. It is related to the displacement of frontal zones and to local hydrological anomalies. Since the completion of the CZCS work there has been a 10 years gap in monitoring chlorophyll spatial distribution dynamics in the ocean. The reason is the long absence of equipment on the orbit, which would be suitable for the measurement of chlorophyll concentration. Recently, SeaWiFS data have been accumulated and their quantity is sufficient for the crude statistical analysis of image time series for 25 years. Thus, it has become possible to obtain data on the long-term stability of quasistationary areas and, therefore, on the stability of large-scale hydrological phenomena in the Atlantic Ocean. В© 2003 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics of SB RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Inst Comp Modeling of SB RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shevyrnogov, A.; Vysotskaya, G.; Shevyrnogov, E.

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Estimation of the stability of the photosynthetic unit in the bioregenerative life support system with plant wastes included in mass exchange / A. A. Tikhomirov [et al.] // Acta Astronautica. - 2008. - Vol. 63, Is. 7-10. - P1111-1118, DOI 10.1016/j.actaastro.2007.12.025 . - ISSN 0094-5765
Кл.слова (ненормированные):
BLSS -- Mass exchange -- Plant wastes -- Agricultural products -- Air pollution -- Biological materials -- Biomass -- Environmental engineering -- Estimation -- Experiments -- Human engineering -- Incineration -- Renewable energy resources -- Space research -- Substrates -- System stability -- Bioregenerative -- Life-support systems -- Mass exchanges -- Plant biomass -- Plant productivity -- Plant wastes -- Radish plants -- Significant reduction -- Soil like substrates -- Wheat straws -- Waste incineration
Аннотация: The purpose of this study is to estimate the possible effect produced on plant productivity by inedible plant biomass added to soil-like substrate (SLS). Results of the experiments with radish plants grown on the SLS with inedible biomass of carrot and beet plants added in the amounts roughly equal to their yields harvested from the same area showed a significant reduction in productivity of radish plants. The yield of radish plants grown on the SLS with added radish tops was almost the same as the yield of the radish grown on the neutral substrate. Experiments with addition of dry wheat straw to the SLS and growing of wheat and radish plants on that substrate also showed that the productivity of the plants grown in that way was decreased. Attempts to negate the adverse effect of plant wastes proved that the most effective way was to mineralize the wastes using the technique of "wet incineration". В© 2008 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics Russian Academy of Sciences, Siberian Branch, Akademgorodok, 660036 Krasnoyarsk, Russian Federation
Environmental Control and Life Support Section, ESA-ESTEC, Postbus 299, 2200 AG Noordwijk, Netherlands
Institute of Biology Komi SC, Ural Branch Russian Academy of Sciences, Kommunisticheskaya Street, 28, 167982 Siktivkar, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Ushakova, S.A.; Velichko, V.V.; Zolotukhin, I.G.; Shklavtsova, E.S.; Lasseur, C.; Golovko, T.K.

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Tolerance of LSS plant component to elevated temperatures / S. A. Ushakova, A. A. Tikhomirov // Acta Astronautica. - 2002. - Vol. 50, Is. 12. - P759-764, DOI 10.1016/S0094-5765(02)00010-3 . - ISSN 0094-5765
Кл.слова (ненормированные):
Metabolism -- Photosynthesis -- Rate constants -- Thermal effects -- Thermoanalysis -- Thermal tolerance -- Life support systems (spacecraft) -- carbon dioxide -- adaptation -- article -- Brassicaceae -- comparative study -- heat -- instrumentation -- light -- metabolism -- microclimate -- photosynthesis -- physiology -- plant physiology -- wheat -- Adaptation, Physiological -- Brassicaceae -- Carbon Dioxide -- Ecological Systems, Closed -- Heat -- Life Support Systems -- Light -- Photosynthesis -- Plant Physiology -- Triticum
Аннотация: Stability of LSS based on biological regeneration of water, air and food subject to damaging factors is largely dependent on the behavior of the photosynthesizing component represented, mainly, by higher plants. The purpose of this study is to evaluate the tolerance of uneven-aged wheat and radish cenoses to temperature effects different in time and value. Estimation of thermal tolerance of plants demonstrated that exposure for 20 h to the temperature increasing to 45В°C brought about irreversible damage both in photosynthetic processes (up to 80% of initial value) and the processes of growth and development. Kinetics of visible photosynthesis during exposure to elevated temperatures can be used to evaluate critical exposure time within the range of which the damage of metabolic processes is reversible. With varying light intensity and air temperature it is possible to find a time period admissible for the plants to stay under adverse conditions without considerable damage of metabolic processes. В© 2002 Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics (Russian Academy of Sciences, Siberian Branch) Academgorodok, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Ushakova, S.A.; Tikhomirov, A.A.

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Stability of recombinant plasmids in transgenic microorganisms under different environmental conditions / L. Yu. Popova [и др.] // Mikrobiologiya. - 2001. - Vol. 70, Is. 6. - С. 796-803 . - ISSN 0026-3656
Кл.слова (ненормированные):
Antibiotic resistance -- Bioluminescence interferon -- Recombinant plasmids -- Transgenic microorganisms -- article -- Bacillus subtilis -- culture medium -- Escherichia coli -- genetic engineering -- genetic recombination -- genetics -- R factor -- Bacillus subtilis -- Culture Media -- Escherichia coli -- Genetic Engineering -- R Factors -- Recombination, Genetic
Аннотация: The copy number of R plasmids weakly depends on the selective pressure of the respective antibiotic but does depend on the physiology of the host species and the type of plasmids and cloned genes, whose expression leads to a further load on the biosynthetic apparatus of cells. The last factor is critical in the maintenance of recombinant plasmids in transgenic microorganisms.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Popova, L.Yu.; Maksimova, E.E.; Lobova, T.I.; Kargatova, T.V.; Boyandin, A.N.; Krylova, T.Yu.; Pechurkin, N.S.

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Stability of recombinant plasmids in transgenic microorganisms under different environmental conditions / L. Yu. Popova [et al.] // Microbiology. - 2001. - Vol. 70, Is. 6. - P685-691 . - ISSN 0026-2617
Кл.слова (ненормированные):
Antibiotic resistance -- Bioluminescence -- Interferon -- Recombinant plasmids -- Transgenic microorganisms
Аннотация: The copy number of R plasmids weakly depends on the selective pressure of the respective antibiotic but does depend on the physiology of the host species and the type of plasmids and cloned genes, whose expression leads to a further load on the biosynthetic apparatus of cells. The last factor is critical in the maintenance of recombinant plasmids in transgenic microorganisms. В© 2001 MAIK "Nauka/Interperiodica".

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Popova, L.Yu.; Maksimova, E.E.; Lobova, T.I.; Kargatova, T.V.; Boyandin, A.N.; Krylova, T.Yu.; Pechurkin, N.S.

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Functional, regulatory and indicator features of microorganisms in man-made ecosystems / L. A. Somova, N. S. Pechurkin // Advances in Space Research. - 2001. - Vol. 27, Is. 9. - P1563-1570, DOI 10.1016/S0273-1177(01)00247-2 . - ISSN 0273-1177
Кл.слова (ненормированные):
Biomass -- Carbon dioxide -- Ecosystems -- Life support systems (spacecraft) -- Photosynthesis -- Sewage treatment -- Soils -- Human microfloras -- Microorganisms -- carbon -- carbon dioxide -- artificial ecosystem -- article -- biomass -- bioreactor -- ecosystem -- human -- intestine -- metabolism -- microbiology -- microclimate -- plant root -- sewage -- wheat -- Biomass -- Bioreactors -- Carbon -- Carbon Dioxide -- Ecological Systems, Closed -- Ecosystem -- Environmental Microbiology -- Humans -- Intestines -- Life Support Systems -- Plant Roots -- Sewage -- Triticum -- Waste Disposal, Fluid
Аннотация: Functional, regulatory and indicator features of microorganisms in development and functioning of the systems and sustaining stability of three man-made ecosystem types has been studied. 1) The functional (metabolic) feature was studied in aquatic ecosystems of biological treatment of sewage waters for the reducer component. 2) The regulatory feature of bacteria for plants (producer component) was studied in simple terrestrial systems "wheat plants-rhizospheric microorganisms - artificial soil" where the behavior of the system varied with activity of the microbial component. For example with atmospheric carbon dioxide content elevated microbes promote intensification of photosynthesis processes, without binding the carbon in the plant biomass. 3) The indicator feature for the humans (consumer component) was studied in Life Support Systems (LSS). High sensitivity of human microflora to system conditions allowed its use as an indicator of the state of both system components and the entire ecosystem. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Somova, L.A.; Pechurkin, N.S.

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Methodology of biospherics for theoretical sciences and practical use. / N. S. Pechurkin, T. Maryasova // Life support & biosphere science : international journal of earth space. - 2000. - Vol. 7, Is. 2. - P219-224 . - ISSN 1069-9422
Кл.слова (ненормированные):
article -- biomass -- ecosystem -- energy metabolism -- food chain -- microclimate -- theoretical model -- Biomass -- Ecological Systems, Closed -- Ecosystem -- Energy Metabolism -- Food Chain -- Life Support Systems -- Models, Theoretical
Аннотация: This article deals with some methodological aspects of biospherics connected with theoretical sciences development and prospective use for practical application. Properties of experimental objects, methods and goals of biospherics as synthesising science have been discussed. The problem of stability of incomplete (natural and artificial) ecosystems has been considered. The concept of the ecosystem health based on effective functioning of different types of ecosystems has been developed.

Scopus
Держатели документа:
Institute of Biophysics, SBRAS, Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pechurkin, N.S.; Maryasova, T.

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10.

Populational stability of the luminescent bacterium Photobacterium leiognathi in continuous culture / N. S. Pechurkin [et al.] // Doklady Biological Sciences. - 1987. - Vol. 294, Is. 1-6. - P269-272 . - ISSN 0012-4966
Кл.слова (ненормированные):
animal cell -- nonhuman -- photobacterium leiognathi

Scopus
Держатели документа:
Institute of Biophysics, Academy of Sciences of the USSR, Krasnoyarsk, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pechurkin, N.S.; Brilkov, A.V.; Bogucharov, A.A.; Pisman, T.I.; Gitelzon, I.I.

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11.

^a343.31.33^2VINITI
П 31

Печуркин, Н. С.
Популяционные аспекты биотехнологии [Текст] : научное издание / Н. С. Печуркин, А. В. Брильков // Abh. Akad. Wiss. DDR Abt. Math., Naturwiss., Techn. - 1982. - N 2. - С. 197-211 . - ISSN 0138-1059

ГРНТИ

34.31.33

РУБ 343.31.33
Рубрики:
БИОТЕХНОЛОГИЯ
   ПОПУЛЯЦИОННЫЕ АСПЕКТЫ
   МИКРОБИОЛОГИЧЕСКИЕ ПРОЦЕССЫ
   СТАБИЛЬНОСТЬ
   ДЛИТЕЛЬНАЯ ФЕРМЕНТАЦИЯ
   ОБЗОРЫ
   BIOTECHNOLOGY
   MICROBIOLOGICAL PROCESSES
   STABILITY
   REVIEW
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Брильков, А.В.

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12.

^a621.37.99^2VINITI
И 88

Исследование фенотипической стабильности рекомбинантного штамма Escherichia coli, содержащего гены люминесцентной системы фотобактерий, при длительном культивировании в хемостате [Текст] : научное издание / Т. И. Письман [и др.] // Биотехнология. - 1991. - N 5. - С. 29-32 . - ISSN 0234-2758

ГРНТИ

62.37.99

РУБ 621.37.99
Рубрики:
ESCHERICHIA COLI
   РЕКОМБИНАНТНЫЕ МИКРООРГАНИЗМЫ
   ФЕНОТИПИЧЕСКАЯ СТАБИЛЬНОСТЬ
   КУЛЬТИВИРОВАНИЕ
   ДЛИТЕЛЬНОЕ
   ХЕМОСТАТ
   ГЕНЫ
   ГЕНЫ ЛЮМИНЕСЦЕНТНОЙ СИСТЕМЫ ФОТОБАКТЕРИЙ
   ESCHERICHIA COLI
   RECOMBINANT
   STABILITY
Аннотация: Изучена стабильность рекомбинантного штамма Escherichia coli Z905, несущего гибридную плазмиду pPHL(Ap{r} Lux{+}), которая содержит гены люминесцентной системы Photobacterium letognathi, клонированные в векторе pUC18. Люминесценция этого штамма подвержена сАМР зависимой катаболитной репрессии. При лимитировании роста популяции бактерий в хемостате как глюкозой, так и глицерином в присутствии антибиотика (50 мкг/мл) доля плазмидсодержащих клеток в популяции за 8-10 генераций резко снижается до 1% и ниже. После 20 генераций обнаружены клетки, образующие несветящиеся, но устойчивые к ампициллину колонки (Ap{r} Lux{-}). При лимитировании роста глюкозой их доля не превышает 0,01%, а глицерином - 18%. Эти клетки содержат плазмиды, размер которых меньше на величину клонированного фрагмента. Библ. 27. Россия Ин-т биофизики СО АН СССР, Красноярск, СНГ.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Письман, Т.И.; Луцкая, Н.И.; Брильков, А.В.; Печуркин, Н.С.

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13.

Immunoelectronmicroscopic study of the nucleoid structure of hydrogen bacteria. / O. A. Mogilnaya [et al.] // Journal of Basic Microbiology. - 1992. - Vol. 32, Is. 6. - P381-387 . - ISSN 0233-111X
Кл.слова (ненормированные):
bacterial DNA -- Alcaligenes -- article -- cell division -- cell nucleus -- Escherichia coli -- immunoelectron microscopy -- ultrastructure -- Alcaligenes -- Cell Division -- Cell Nucleus -- DNA, Bacterial -- Escherichia coli -- Microscopy, Immunoelectron
Аннотация: Electron microscopical studies of the nucleoid structure of hydrogen bacteria using ultrahin sections and spread DNA from bacterial cell lysates revealed a different DNA packaging in the cell. A compact state of the major part of DNA at all growth stages and stability of nucleosome-like structures were shown. The use of antibodies to HU protein of E. coli labelled by protein A-colloidal gold demonstrated the immunological relationship between HU protein of E. coli and histone-like proteins of Alcaligenes eutrophus and their possible role in the nucleosome-like DNA packaging in procariotic genome.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Mogilnaya, O.A.; Kiselyova, E.V.; Medvedeva, S.E.; Puzir, A.P.; Guseynov, O.A.; Kulyba, N.N.; Kozlov, A.V.

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14.

Dynamics of forest insect density: Bifurcation approach / A. D. Bazykin [et al.] // Journal of Theoretical Biology. - 1997. - Vol. 186, Is. 3. - P267-278, DOI 10.1006/jtbi.1996.0363 . - ISSN 0022-5193
Кл.слова (ненормированные):
article -- ecology -- forest -- insect -- nonhuman -- population density -- population dynamics -- population model -- priority journal
Аннотация: Six basic phase portraits differing in character and in the number of their equilibrium behaviour regimes were suggested in the classification of forest insect population dynamics. The portraits graphically illustrate six types of dynamic behavior of numbers of the system 'phytophage-entomophage': two types of stable equilibrium dynamics (stationary and oscillatory) and four types corresponding to outbreaks of mass reproduction - fixed, permanent, reversive and true outbreaks, respectively. In this paper we consider the single parameter model, with its parametric domains realizing a wide spectrum of phase dynamics, both basic and transitional. The changes in the phase portraits are accompanied by the bifurcations in the model. The results of these analyses are in agreement with the 'stability principle of mobile ecological systems' and may supply its parameter substantiation at the model level.

Scopus
Держатели документа:
Inst. of Math. Problem Biology, Russian Academy of Sciences, Puschino Moscow Region, 142292, Russian Federation
Ctr. of Productivity and Forest E., Russian Academy of Sciences, Novocheremushkinskaya, 69, Moscow 117418, Russian Federation
Institute of Biophysics S.B., Russ. Acad. of Sciences, Krasnoyarsk, Akademgorodok 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bazykin, A.D.; Berezovskaya, F.S.; Isaev, A.S.; Khlebopros, R.G.

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15.

Characterization of field emission cathodes with different forms of diamond coatings [Text] / V. V. Zhirnov [et al.] // J. Vac. Sci. Technol. B. - 1999. - Vol. 17: 11th International Vacuum Microelectronics Conference (IVMC 98) (JUL 19-23, 1998, ASHEVILLE, NORTH CAROLINA), Is. 2. - P666-669, DOI 10.1116/1.590614. - Cited References: 9 . - 4. - ISSN 1071-1023

РУБ Engineering, Electrical & Electronic + Nanoscience & Nanotechnology + Physics, Applied
Рубрики:
TIPS
   SILICON
   GROWTH
   ARRAYS
   FILMS
Аннотация: The emission of different, diamond coatings on silicon field emitter. arrays were investigated: chemical vapor deposition diamond, high pressure and high temperature synthetic diamond, and shock-synthesized nanodiamond. Practical emission characteristics such as: emission threshold, maximum current, current stability, and reproducibility were tested. The effects of surface modification, the size of diamond crystallites, and hydrogen plasma treatments were also studied. (C) 1999 American Vacuum Society. [S0734-211X(99)06102-8].

Держатели документа:
Inst Crystallog, Moscow 117333, Russia
Univ Fribourg, Dept Phys, CH-1700 Fribourg, Switzerland
Russian Fed Nucl Ctr, Snezhinsk 456770, Russia
Inst Biophys, Krasnoyarsk 660036, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zhirnov, V.V.; Kuttel, O.M.; Groning, O...; Alimova, A.N.; Detkov, P.Y.; Belobrov, P.I.; Maillard-Schaller, E...; Schlapbach, L...

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16.

EFFECT OF TEMPERATURE ON ACTIVITY AND STABILITY OF OBELIN [Text] / V. S. BONDAR [et al.] // Biochem.-Moscow. - 1992. - Vol. 57, Is. 7. - P717-724. - Cited References: 15 . - 8. - ISSN 0006-2979

РУБ Biochemistry & Molecular Biology
Рубрики:
CA-2+
PHOTOPROTEINS
INDICATORS
Кл.слова (ненормированные):
PHOTOPROTEINS -- OBELIN -- ACTIVATION ENERGY -- THERMOINACTIVATION -- THERMOSTABILITY
Аннотация: The temperature dependence of bioluminescent activity of the Ca2+-activated photoprotein obelin from the hydroid polyp Obelia longissima and thermoinactivation of this protein at different concentrations of (NH4)2SO4 have been studied. The maximal intensity of luminescence of obelin was observed at 4-15-degrees-C. The activity of the photoprotein is completely stable to storage for 3 days at room temperature. Increasing the temperature to 40-degrees-C resulted in a 25-30% loss of enzyme activity in 1 h. The presence of ammonium sulfate during heating stabilizes the activity of obelin. Two breaks, at 11 +/- 3-degrees-C and 47 +/- 3-degrees-C, are observed in the Arrhenius plot of the first-order rate constant of the luminescence decay. The bioluminescent curves of obelin are biphasic in the temperature range 10-40-degrees-C. It is assumed that obelin may exist in two kinetically distinct conformers (active and inactive) whose ratio is temperature dependent.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
BONDAR, V.S.; TROFIMOV, K.P.; SANDALOV, T.P.; VYSOTSKII, E.S.

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17.

Ligand binding and conformational states of the photoprotein obelin / E. V. Eremeeva [et al.] // FEBS Lett. - 2012. - Vol. 586, Is. 23. - P4173-4179, DOI 10.1016/j.febslet.2012.10.015. - Cited References: 24. - The work was supported by RFBR grant 12-04-00131, by the Program of the Government of Russian Federation "Measures to Attract Leading Scientists to Russian Educational Institutions" (grant 11.G34.31.058), by the Program "Molecular and Cellular Biology" of RAS. The Wageningen University Sandwich PhD-Fellowship Program supported E.V.E. . - ISSN 0014-5793

РУБ Biochemistry & Molecular Biology + Biophysics + Cell Biology
Рубрики:
RECOMBINANT OBELIN
   CRYSTAL-STRUCTURE
   LIGHT-EMISSION
   APO-AEQUORIN
   BIOLUMINESCENCE
   COELENTERAZINE
   LUMINESCENCE
   STABILITY
   ANGSTROM
   PROTEINS
Кл.слова (ненормированные):
Bioluminescence -- Coelenterazine -- Photoprotein -- Thermostability
Аннотация: Many proteins require a non-covalently bound ligand to be functional. How ligand binding affects protein conformation is often unknown. Here we address thermal unfolding of the free and ligand-bound forms of photoprotein obelin. Fluorescence and far-UV circular dichroism ( CD) data show that the various ligand-dependent conformational states of obelin differ significantly in stability against thermal unfolding. Binding of coelenterazine and calcium considerably stabilizes obelin. In solution, all obelin structures are similar, except for apo-obelin without calcium. This latter protein is an ensemble of conformational states, the populations of which alter upon increasing temperature. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

Держатели документа:
[Eremeeva, Elena V.
Westphal, Adrie H.
van Mierlo, Carlo P. M.
van Berkel, Willem J. H.] Wageningen Univ, Biochem Lab, NL-6703 HA Wageningen, Netherlands
[Eremeeva, Elena V.
Vysotski, Eugene S.] Russian Acad Sci, Photobiol Lab, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
[Eremeeva, Elena V.
Vysotski, Eugene S.] Siberian Fed Univ, Lab Bioluminescence Biotechnol, Inst Fundamental Biol & Biotechnol, Krasnoyarsk 660041, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Eremeeva, E.V.; Vysotski, E.S.; Westphal, A.H.; van Mierlo, CPM; van Berkel, WJH

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18.

Coelenterazine-v ligated to Ca2+-triggered coelenterazine-binding protein is a stable and efficient substrate of the red-shifted mutant of Renilla muelleri luciferase [Text] / G. A. Stepanyuk [et al.] // Anal. Bioanal. Chem. - 2010. - Vol. 398, Is. 4. - P1809-1817, DOI 10.1007/s00216-010-4106-9. - Cited References: 39. - This work was supported by grant 09-04-12022 of the Russian Foundation for Basic Research, "Molecular and Cell Biology" program of Russian Academy of Sciences, by the SB RAS grant No. 2, and by the SB RAS Lavrentiev grant for Young Scientists. . - ISSN 1618-2642

РУБ Biochemical Research Methods + Chemistry, Analytical
Рубрики:
GREEN-FLUORESCENT PROTEIN
   BIOLUMINESCENT REPORTER
   CA2+-REGULATED PHOTOPROTEINS
   RENIFORMIS LUCIFERASE
   RECOMBINANT OBELIN
   GENE-EXPRESSION
   IN-VIVO
   CDNA
   CLONING
   PURIFICATION
Кл.слова (ненормированные):
Bioluminescence -- Coelenterazine -- Calcium -- Imaging
Аннотация: It has been shown that the coelenterazine analog, coelenterazine-v, is an efficient substrate for a reaction catalyzed by Renilla luciferase. The resulting bioluminescence emission maximum is shifted to a longer wavelength up to 40 nm, which allows the use of some "yellow" Renilla luciferase mutants for in vivo imaging. However, the utility of coelenterazine-v in small-animal imaging has been hampered by its instability in solution and in biological tissues. To overcome this drawback, we ligated coelenterazine-v to Ca2+-triggered coelenterazine-binding protein from Renilla muelleri, which apparently functions in the organism for stabilizing and protecting coelenterazine from oxidation. The coelenterazine-v bound within coelenterazine-binding protein has revealed a greater long-term stability at both 4 and 37 degrees C. In addition, the coelenterazine-binding protein ligated by coelenterazine-v yields twice the total light over free coelenterazine-v as a substrate for the red-shifted R. muelleri luciferase. These findings suggest the possibility for effective application of coelenterazine-v in various in vitro assays.

Держатели документа:
[Stepanyuk, Galina A.
Malikova, Natalia P.
Markova, Svetlana V.
Vysotski, Eugene S.] Russian Acad Sci, Inst Biophys, Siberian Branch, Photobiol Lab, Krasnoyarsk 660036, Russia
[Unch, James] Promega Biosci LLC, San Luis Obispo, CA 93401 USA
[Lee, John] Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Stepanyuk, G.A.; Unch, J...; Malikova, N.P.; Markova, S.V.; Lee, J...; Vysotski, E.S.

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19.

Recombinant Metridia luciferase isoforms: expression, refolding and applicability for in vitro assay [Text] / V. V. Borisova [et al.] // Photochem. Photobiol. Sci. - 2008. - Vol. 7, Is. 9. - P1025-1031, DOI 10.1039/b807271j. - Cited References: 19. - The work was supported by Bayer AG, by the Russian Foundation for Basic Research grants 05-04-48271 and 06-04-08076, by the joint grant 06-04-89502 of the Russian Foundation for Basic Research and Taiwan National Science Council, and by the "Molecular and Cellular Biology" program of the Russian Academy of Sciences. . - ISSN 1474-905X

РУБ Biochemistry & Molecular Biology + Biophysics + Chemistry, Physical
Рубрики:
BIOLUMINESCENT REPORTER
   GAUSSIA LUCIFERASE
   CDNA
   PROTEINS
   CLONING
   OVEREXPRESSION
   PURIFICATION
   MUTAGENESIS
   ENZYME
   OBELIN
Аннотация: The recombinant coelenterazine-dependent luciferases (isoforms MLuc 164 and MLuc39) from the marine copepod Metridia longa were expressed as inclusion bodies in E. coli cells, dissolved in 6 M guanidinium chloride and folded in conditions developed for proteins containing intramolecular disulfide bonds. One of them (MLuc09) was obtained in an active monomeric form with a high yield. The luciferase bioluminescence is found to be initiated not only by free coelenterazine, but also by Ca2+-dependent coelenterazine-binding protein (CBP) of Renilla muelleri on Ca2+ addition. The use of CBP as a "substrate" provides higher light emission and simultaneously the lower level of background. The high purity MLuc39 can be detected down to attomol with a linear range extending over 5 orders of magnitude. The MLuc39 reveals also a high stability towards heating and chemical modification; the chemically synthesized biotinylated derivatives of the luciferase preserve 35-40% of the initial activity The luciferase applicability as an in vitro bioluminescent reporter is demonstrated in model tandem bioluminescent solid-phase microassay combining the Ca2+-regulated photoprotein obelin and the Metridia luciferase.

Держатели документа:
[Borisova, Vasillisa V.
Frank, Ludmila A.
Markova, Svetlana V.
Burakova, Ludmilla P.
Vysotski, Eugene S.] Russian Acad Sci, Inst Biophys, Photobiol Lab, Siberian Branch, Krasnoyarsk 660036, Russia
[Frank, Ludmila A.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Borisova, V.V.; Frank, L.A.; Markova, S.V.; Burakova, L.P.; Vysotski, E.S.

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20.

Violet and greenish photoprotein obelin mutants for reporter applications in dual-color assay [Text] / L. A. Frank [et al.] // Anal. Bioanal. Chem. - 2008. - Vol. 391, Is. 8. - P2891-2896, DOI 10.1007/s00216-008-2223-5. - Cited References: 22 . - ISSN 1618-2642

РУБ Biochemical Research Methods + Chemistry, Analytical
Рубрики:
ANGSTROM RESOLUTION
   RECOMBINANT OBELIN
   CRYSTAL-STRUCTURE
   BIOLUMINESCENCE
   AEQUORIN
   IMMUNOASSAY
   EXPRESSION
   CDNA
   PURIFICATION
   CLONING
Кл.слова (ненормированные):
Ca(2+)-regulated photoprotein -- bioluminescence -- dual-color assay
Аннотация: Two kinds of Ca(2+)-regulated photoprotein obelin with altered color of bioluminescence were obtained by active-center amino acid substitution. The mutant W92F-H22E emits violet light (lambda(max)=390 nm) and the mutant Y139F emits greenish light (lambda (max)=498 nm), with small spectral overlap, both display high activity and stability and thus may be used as reporters. For demonstration, the mutants were applied in dual-color simultaneous immunoassay of two gonadotropic hormones-follicle-stimulating hormone and luteinizing hormone. Bioluminescence of the reporters was simultaneously triggered by single injection of Ca(2+) solution, divided using band-pass optical filters and measured with a two-channel photometer. The sensitivity of simultaneous bioluminescence assay was close to that of a separate radioimmunoassay.

Держатели документа:
[Frank, Ludmila A.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
[Frank, Ludmila A.
Borisova, Vasilisa V.
Markova, Svetlana V.
Malikova, Natalia P.
Stepanyuk, Galina A.
Vysotski, Eugene S.] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Frank, L.A.; Borisova, V.V.; Markova, S.V.; Malikova, N.P.; Stepanyuk, G.A.; Vysotski, E.S.

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