Библиотека института биофизики СО РАН

Главная

Базы данных

Труды сотрудников ИБФ СО РАН - результаты поиска

Вид поиска

Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН

Иностранные журналы библиотеки Института биофизики СО РАН

Отечественные журналы библиотеки Института биофизики СО РАН

Каталог диссертаций ИБФ СО РАН

Труды сотрудников ИБФ СО РАН

Область поиска

в найденном

Найдено в других БД:

Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН (1)

Формат представления найденных документов:
полный	информационный	краткий

Отсортировать найденные документы по:
автору	заглавию	году издания	типу документа

Поисковый запрос: (<.>K=Substrates<.>)

Общее количество найденных документов : 65
Показаны документы с 1 по 20

1-20 21-40 41-60 61-65

Cell growth and accumulation of polyhydroxyalkanoates from CO2 and H2 of a hydrogen-oxidizing bacterium, Cupriavidus eutrophus B-10646 / T. G. Volova [et al.] // Bioresource Technology. - 2013. - Vol. 146. - P215-222, DOI 10.1016/j.biortech.2013.07.070 . - ISSN 0960-8524
Кл.слова (ненормированные):
Autotrophic synthesis -- Cupriavidus eutrophus -- Polyhydroxyalkanoates
Аннотация: Synthesis of polyhydroxyalkanoates (PHAs) by a new strain of Cupriavidus - Cupriavidus eutrophus B-10646 - was investigated under autotrophic growth conditions. Under chemostat, at the specific flow rate D=0.1h-1, on sole carbon substrate (CO2), with nitrogen, sulfur, phosphorus, potassium, and manganese used as growth limiting elements, the highest poly(3-hydroxybutyrate) [P(3HB)] yields were obtained under nitrogen deficiency. In batch autotrophic culture, in the fermenter with oxygen mass transfer coefficient 0.460h-1, P(3HB) yields reached 85% of dry cell weight (DCW) and DCW reached 50g/l. Concentrations of supplementary PHA precursor substrates (valerate, hexanoate, ?-butyrolactone) and culture conditions were varied to produce, for the first time under autotrophic growth conditions, PHA ter- and tetra-polymers with widely varying major fractions of 3-hydroxybutyrate, 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate monomer units. Investigation of the high-purity PHA specimens showed significant differences in their physicochemical and physicomechanical properties. В© 2013 Elsevier Ltd.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
L.V. Kirenckii Institute of Physics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Institute of Chemistry and Chemical Technology of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kiselev, E.G.; Shishatskaya, E.I.; Zhila, N.O.; Boyandin, A.N.; Syrvacheva, D.A.; Vinogradova, O.N.; Kalacheva, G.S.; Vasiliev, A.D.; Peterson, I.V.

Найти похожие

Experimental determination of organic substrates utilized by noncultivable bacterioplankton of a eutrophic reservoir / M. Yu. Trusova, M. I. Gladyshev // Doklady Biological Sciences. - 2006. - Vol. 409, Is. 1. - P302-304, DOI 10.1134/S0012496606040090 . - ISSN 0012-4966

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Krasnoyarsk State University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Trusova, M.Yu.; Gladyshev, M.I.

Найти похожие

Surface properties of nanodiamond films deposited by electrophoresis on Si(100) / E. Maillard-Schaller [et al.] // Diamond and Related Materials. - 1999. - Vol. 8, Is. 2-5. - P805-808 . - ISSN 0925-9635
Кл.слова (ненормированные):
Energy band diagram -- Nanodiamond -- Raman spectroscopy -- Surface characterization -- Band structure -- Electrodeposition -- Electrophoresis -- Hydrogen -- Nanostructured materials -- Nitrogen -- Oxidation -- Oxygen -- Phonons -- Plasma applications -- Silicon wafers -- Surface properties -- Dielectrophoresis -- Negative electron affinity (NEA) -- Phonon confinement effect -- Diamond films
Аннотация: The surface properties of diamond nanoparticles (40-50 A in diameter) have been investigated by X-ray photoelectron spectroscopy (XPS), UV photoelectron spectroscopy (UPS) and Raman spectroscopy. The diamond nanoparticles have been deposited on flat Si(100) substrates by electrophoresis/dielectrophoresis. The as-deposited films are strongly oxidized and present a 1-2% nitrogen content. After treatment at 850 В°C in H2 plasma for 60 min, the oxygen is removed, and the position of the C 1s core-level peak indicates a n-type electronic comportment of the diamond nanoparticles. Raman spectroscopy of the as-deposited film shows a sp3 contribution at 1321 cm-1 and a sp2 contribution around 1620 cm-1. The 12 cm-1 shift of the sp3 contribution with respect to the bulk diamond peak at 1333 cm-1 is attributed to a phonon confinement effect due to the size of the diamond particles. The H2 plasma treatment induces a size decrease of the nanocrystallites confirmed by Raman and scanning electron microscopy (SEM) measurements. UPS spectroscopy shows a negative electron affinity of -0.2 eV of the hydrogenated nanodiamond film.

Scopus
Держатели документа:
Solid State Physics Department, University of Fribourg, 1700, Fribourg, Switzerland
Institute of Christallography, 117333, Moscow, Russian Federation
Institute of Biophysics, 660036, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Maillard-Schaller, E.; Kuettel, O.M.; Diederich, L.; Schlapbach, L.; Zhirnov, V.V.; Belobrov, P.I.

Найти похожие

Production characteristics of the "higher plants-soil-like substrate" system as an element of the bioregenerative life support system / V. V. Velichko [et al.] // Advances in Space Research. - 2013. - Vol. 51, Is. 1. - P115-123, DOI 10.1016/j.asr.2012.08.003 . - ISSN 0273-1177
Кл.слова (ненормированные):
Biological-technical life support system -- Soil-like substrate -- Utilization of plant wastes -- Age groups -- Bioregenerative life support systems -- Cultivated crops -- Cyperus esculentus -- Growth chamber -- Higher plants -- Mineral element -- Nitrogen content -- Nutrient solution -- Plant wastes -- Raphanus sativus -- Root zone -- Soil-like substrate -- Study materials -- Biomass -- Carbon dioxide -- Harvesting -- Minerals -- Plants (botany) -- Soils -- Substrates -- Waste utilization -- Ecology
Аннотация: The study addresses the possibility of long-duration operation of a higher plant conveyor, using a soil-like substrate (SLS) as the root zone. Chufa (Cyperus esculentus L.), radish (Raphanus sativus L.), and lettuce (Lactuca sativa L.) were used as study material. A chufa community consisting of 4 age groups and radish and lettuce communities consisting of 2 age groups were irrigated with a nutrient solution, which contained mineral elements extracted from the SLS. After each harvest, inedible biomass of the harvested plants and inedible biomasses of wheat and saltwort were added to the SLS. The amounts of the inedible biomasses of wheat and saltwort to be added to the SLS were determined based on the nitrogen content of the edible mass of harvested plants. CO2 concentration in the growth chamber was maintained within the range of 1100-1700 ppm. The results of the study show that higher plants can be grown quite successfully using the proposed process of plant waste utilization in the SLS. The addition of chufa inedible biomass to the SLS resulted in species-specific inhibition of growth of both cultivated crops and microorganisms in the "higher plants - SLS" system. There were certain differences between the amounts of some mineral elements removed from the SLS with the harvested edible biomass and those added to it with the inedible biomasses of wheat and saltwort. В© 2012 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of Russian Academy of Science, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Velichko, V.V.; Tikhomirov, A.A.; Ushakova, S.A.; Tikhomirova, N.A.; Shihov, V.N.; Tirranen, L.S.; Gribovskaya, I.A.

Найти похожие

Assessment of the possibility of establishing material cycling in an experimental model of the bio-technical life support system with plant and human wastes included in mass exchange / A. A. Tikhomirov [et al.] // Acta Astronautica. - 2011. - Vol. 68, Is. 9-10. - P1548-1554, DOI 10.1016/j.actaastro.2010.10.005 . - ISSN 0094-5765
Кл.слова (ненормированные):
Biological-technical life support system -- Photosynthesizing unit -- Utilization of plant and human wastes -- Biological substrates -- Chemical component -- Experimental models -- Human waste -- Life support systems -- Mass exchange -- Mass transfer process -- Material cycling -- Photosynthesizing unit -- Physicochemical methods -- Pilot model -- Plant biomass -- Plant wastes -- Recycled products -- Salicornia europaea -- Simultaneous use -- Soil-like substrate -- Utilization of plant and human wastes -- Sodium chloride -- Substrates -- Waste incineration -- Waste utilization
Аннотация: A pilot model of a bio-technical life support system (BTLSS) including human and plant wastes has been developed at the Institute of Biophysics SB RAS (Krasnoyarsk, Russia). This paper describes the structure of the photosynthesizing unit of the system, which includes wheat, chufa and vegetables. The study substantiates the simultaneous use of neutral and biological substrates for cultivating plants. A novel physicochemical method for the involvement of human wastes in the cycling has been employed, which enables the use of recycled products as nutrients for plants. Inedible plant biomass was subjected to biological combustion in the soil-like substrate (SLS) and was thus involved in the system mass exchange; NaCl contained in native urine was returned to the human through the consumption of Salicornia europaea, an edible salt-concentrating plant. Mass transfer processes in the studied BLSS have been examined for different chemical components. В© 2009 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
SB RAS Institute of Biophysics, Akademgorodok 50/50, Krasnoyarsk 660036, Russian Federation
Blaise Pascal University, France
ESA-ESTEC, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Ushakova, S.A.; Velichko, V.V.; Tikhomirova, N.A.; Kudenko, Y.A.; Gribovskaya, I.V.; Gros, J.-B.; Lasseur, C.

Найти похожие

Some methods for human liquid and solid waste utilization in bioregenerative life-support systems / S. A. Ushakova [et al.] // Applied Biochemistry and Biotechnology. - 2008. - Vol. 151, Is. 2-3. - P676-685, DOI 10.1007/s12010-008-8291-3 . - ISSN 0273-2289
Кл.слова (ненормированные):
Closure -- Human waste -- Life-support systems -- Salicornia -- Sodium chloride -- Above-ground biomass -- Biological lives -- Bioregenerative -- Closure -- Cultivation process -- Culture methods -- Human waste -- Irrigation waters -- Life-support systems -- Manned space missions -- Mineral elements -- Physico-chemical methods -- Salicornia -- Salicornia europaea -- Salt-tolerant -- Soil-like substrates -- Biomass -- Body fluids -- Electrodialysis -- Grain (agricultural product) -- Irrigation -- Liquids -- Metal refining -- Minerals -- Mining -- Oxidation -- Plant shutdowns -- Sodium chloride -- Soils -- Solid wastes -- Substrates -- Water supply -- Vegetation -- article -- biomass -- controlled study -- electrodialysis -- halophyte -- irrigation (agriculture) -- microclimate -- nonhuman -- recycling -- Salicornia europaea -- solid waste -- bioremediation -- dialysis -- feces -- goosefoot -- growth, development and aging -- human -- methodology -- salt tolerance -- urine -- waste management -- wheat -- Batis maritima -- Salicornia -- Salicornia europaea -- Triticum aestivum -- Biodegradation, Environmental -- Chenopodiaceae -- Dialysis -- Feces -- Humans -- Life Support Systems -- Salt-Tolerance -- Triticum -- Urine -- Waste Management
Аннотация: Bioregenerative life-support systems (BLSS) are studied for developing the technology for a future biological life-support system for long-term manned space missions. Ways to utilize human liquid and solid wastes to increase the closure degree of BLSS were investigated. First, urine and faeces underwent oxidation by Kudenko's physicochemical method. The products were then used for root nutrition of wheat grown by the soil-like substrate culture method. Two means of eliminating sodium chloride, introduced into the irrigation solution together with the products of urine oxidation, were investigated. The first was based on routine electrodialysis of irrigation water at the end of wheat vegetation. Dialysis eliminated about 50% of Na from the solution. This desalinization was performed for nine vegetations. The second method was new: after wheat cultivation, the irrigation solution and the solution obtained by washing the substrate containing mineral elements not absorbed by the plants were used to grow salt-tolerant Salicornia europaea L. plants (saltwort). The above-ground biomass of this plant can be used as a food, and roots can be added to the soil-like substrate. Four consecutive wheat and Salicornia vegetations were cultivated. As a result of this wheat and Salicornia cultivation process, the soil-like substrate salinization by NaCl were considerably decreased. В© 2008 Humana Press.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Science, Siberian Branch, 660036 Krasnoyarsk, Russian Federation
K.A. Timiraziev Institute of Plant Physiology, Russian Academy of Science, 35 Botanisheskaya, 127276 Moscow, Russian Federation
LGCB, Universite Blaise Pascal, Polytech'Clermont-Ferrand, P.O. Box 206, 63174 Aubiere cedex, France : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Ushakova, S.A.; Zolotukhin, I.G.; Tikhomirov, A.A.; Tikhomirova, N.A.; Kudenko, Yu.A.; Gribovskaya, I.V.; Balnokin, Yu.; Gros, J.B.

Найти похожие

Estimation of the stability of the photosynthetic unit in the bioregenerative life support system with plant wastes included in mass exchange / A. A. Tikhomirov [et al.] // Acta Astronautica. - 2008. - Vol. 63, Is. 7-10. - P1111-1118, DOI 10.1016/j.actaastro.2007.12.025 . - ISSN 0094-5765
Кл.слова (ненормированные):
BLSS -- Mass exchange -- Plant wastes -- Agricultural products -- Air pollution -- Biological materials -- Biomass -- Environmental engineering -- Estimation -- Experiments -- Human engineering -- Incineration -- Renewable energy resources -- Space research -- Substrates -- System stability -- Bioregenerative -- Life-support systems -- Mass exchanges -- Plant biomass -- Plant productivity -- Plant wastes -- Radish plants -- Significant reduction -- Soil like substrates -- Wheat straws -- Waste incineration
Аннотация: The purpose of this study is to estimate the possible effect produced on plant productivity by inedible plant biomass added to soil-like substrate (SLS). Results of the experiments with radish plants grown on the SLS with inedible biomass of carrot and beet plants added in the amounts roughly equal to their yields harvested from the same area showed a significant reduction in productivity of radish plants. The yield of radish plants grown on the SLS with added radish tops was almost the same as the yield of the radish grown on the neutral substrate. Experiments with addition of dry wheat straw to the SLS and growing of wheat and radish plants on that substrate also showed that the productivity of the plants grown in that way was decreased. Attempts to negate the adverse effect of plant wastes proved that the most effective way was to mineralize the wastes using the technique of "wet incineration". В© 2008 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics Russian Academy of Sciences, Siberian Branch, Akademgorodok, 660036 Krasnoyarsk, Russian Federation
Environmental Control and Life Support Section, ESA-ESTEC, Postbus 299, 2200 AG Noordwijk, Netherlands
Institute of Biology Komi SC, Ural Branch Russian Academy of Sciences, Kommunisticheskaya Street, 28, 167982 Siktivkar, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Ushakova, S.A.; Velichko, V.V.; Zolotukhin, I.G.; Shklavtsova, E.S.; Lasseur, C.; Golovko, T.K.

Найти похожие

Wheat growth on neutral and soil-like substrates: Carbon dioxide exchange and microflora / J. -B. Gros [et al.] // Acta Horticulturae. - 2004. - Vol. 644. - P243-248
Кл.слова (ненормированные):
Carbon dioxide -- Closed ecological system
Аннотация: The soil-like substrate (SLS), which is a potential candidate for employment in closed ecological systems, has been tested. CO2 exchange, composition and numbers of soil microflora have been examined in .wheat-SLS. system. The results produced have been compared to analogous characteristics of .wheat-neutral substrate. system. A hydroponic method was used under wheat growing on the neutral substrate (expanded clay aggregate). Plants for both studies were grown in closed environment from seed to physiological maturity. In the .wheat-SLS. system, the net photosynthetic rate of canopy was positive in the course of 6.55 days after planting. The net photosynthetic rate of canopy in .wheat-neutral substrate. system was positive in the entire course of vegetation. According to calculations in the course of vegetation, photosynthesis has withdrawn 3.28 kg m-2 CO2 from the .wheat-SLS. system and 3.40 kg m-2 CO2 from the .wheat-neutral substrate. system. On the SLS dominant among bacteria were the spore-forming bacteria from Bacillus genus, among fungi . from Trichoderma genus. In the hydroponic cultivation on neutral substrate dominant were bacteria from Pseudomonas genus, most commonly found fungi were species from Fusarium and Botrytis genera.

Scopus
Держатели документа:
LGCB, Universte B. Pascal, BP206, FR-63174 Aubiere cedex, France
ESA, Estec 2200 AG Noordwijk, Netherlands
Academgorodok, Institute of Biophysics, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gros, J.-B.; Lasseur, C.; Tikhomirov, A.A.; Manukovsky, N.S.; Ushakova, S.A.; Zolotukhin, I.G.; Tirranen, L.S.; Borodina, E.V.; Kovalev, V.S.

Найти похожие

Light intensity and production parameters of phytocenoses cultivated on soil-like substrate under controled environment conditions / A. A. Tikhomirov [et al.] // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1775-1780, DOI 10.1016/S0273-1177(03)80020-0 . - ISSN 0273-1177
Кл.слова (ненормированные):
Bacteria -- Biology -- Crops -- Photosynthesis -- Substrates -- Light intensity -- Space research -- biosphere -- Biomass -- Carbon Dioxide -- Colony Count, Microbial -- Culture Media -- Ecological Systems, Closed -- Enterobacteriaceae -- Life Support Systems -- Light -- Photosynthesis -- Plant Transpiration -- Raphanus -- Soil Microbiology -- Triticum -- Water Microbiology
Аннотация: To increase the degree of closure of biological life support systems of a new generation, we used vermicomposting to involve inedible phytomass in the intra-system mass exchange. The resulting product was a soil-like substrate, which was quite suitable for growing plants (Manukovsky et al. 1996, 1997). However, the soil-like substrate can be regarded as a candidate for inclusion in a system only after a comprehensive examination of its physical, chemical, and other characteristics. An important criterion is the ability of the soil-like substrate to supply the necessary mineral elements to the photosynthesizing component under the chosen cultivation conditions. Thus, the purpose of this work was to study the feasibility of enhancing the production activity of wheat and radish crops by varying the intensity of photosynthetically active radiation, without decreasing the harvest index. The increase of light intensity from 920 to 1150 ?molВ·m -2В·s -1 decreased the intensity of apparent photosynthesis of the wheat crops and slightly increased the apparent photosynthesis of the radish crops The maximum total and grain productivity (kg/m 2) of the wheat crops was attained at the irradiance of 920 ?molВ·m -2В·s -1. Light intensity of 1150 ?molВ·m -2В·s -1 decreased the productivity of wheat plants and had no significant effect on the productivity of the radish crops (kg/m 2) as compared to 920 ?molВ·m 2В·s -1. The qualitative and quantitative composition of microflora of the watering solution and substrate was determined by the condition of plants, developmental phase and light intensity. By the end of wheat growth under 1150 ?molВ·m -2В·s -1 the numbers of bacteria of the coliform family and phytopathogenic bacteria in the watering solution and substrate were an order of magnitude larger than under other illumination conditions. The obtained data suggest that the cultivation of plants in a life support system on soil-like substrate from composts has a number of advantages over the cultivation on neutral substrates, which require continual replenishment of the plant nutrient solution from the system's store to complement the macro- and microelements. Yet, a number of problems arise, including those related to the controlling of the production activity of the plants by the intensity of photosynthetically active radiation. It is essential to understand why the intensity of production processes is limited at higher irradiation levels and to overcome the factors responsible for this, so that the soil-like substrate could have an even better chance in the competition for the best plant cultivation technology to be used in biological life support systems. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, RAS SB, Krasnoyarsk 660036, Russian Federation
Tomsk State University, Lenin street, 36, Tomsk 634050, Russian Federation
LGCB, Universte B. Pascal, BP206, Aubiere cedex 63174, France
ESA/ESTEC, Noordwijk, AG 2200, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Ushakova, S.A.; Gribovskaya, I.A.; Tirranen, L.S.; Manukovsky, N.S.; Zolotukhin, I.G.; Karnachuk, R.A.; Gros, J.-B.; Lasseur, Ch.

Найти похожие

10.

On monitoring the bacterial component as an indicator of the state of small man-made ecosystems / A. B. Sarangova, L. A. Somova, N. S. Pechurkin // Advances in Space Research. - 2001. - Vol. 27, Is. 9. - P1605-1609, DOI 10.1016/S0273-1177(01)00256-3 . - ISSN 0273-1177
Кл.слова (ненормированные):
Bacteria -- Ecosystems -- Substrates -- Intracellular substrate concentration -- Space research -- catalase -- oxidoreductase -- artificial ecosystem -- article -- bacterial phenomena and functions -- biomass -- culture medium -- ecosystem -- enzymology -- growth, development and aging -- metabolism -- microbiology -- oxygen consumption -- Pseudomonas -- Bacterial Physiology -- Biomass -- Catalase -- Culture Media -- Ecosystem -- Oxidoreductases -- Oxygen Consumption -- Pseudomonas -- Water Microbiology
Аннотация: High reproduction rates make the bacterial component of ecosystems a good indicator of the state of the system on the whole. This determines the necessity to develop rapid monitoring of the functional state of the bacterial component of small ecosystems. Information about substrate concentration in the population is indicative of the state of the bacterial culture. Conventional methods of monitoring the concentration of integral substrate in the system take time much longer than the changes in the ecosystem. The paper presents theoretical foundations for the logical sequence "catalase activity - intracellular substrate concentration - estimate of substrate consumed by bacteria" for experimental verification and as a consequence of development of the integral method of monitoring the bacterial population on the basis of determining bacterial catalase activity. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Academgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sarangova, A.B.; Somova, L.A.; Pechurkin, N.S.

Найти похожие

11.

Closed artificial ecosystems as a means of ecosystem studies for earth and space needs / N. S. Pechurkin, I. M. Shirobokova // Advances in Space Research. - 2001. - Vol. 27, Is. 9. - P1497-1504, DOI 10.1016/S0273-1177(01)00244-7 . - ISSN 0273-1177
Кл.слова (ненормированные):
artificial ecosystem -- bioremediation -- biosphere -- ecosystem -- environmental monitoring -- model -- Bioremediation -- Ecology -- Ecosystems -- Health -- Biosphere -- Space research -- artificial ecosystem -- Biodegradation, Environmental -- Earth (Planet) -- Ecological Systems, Closed -- Ecology -- Ecosystem -- Energy Transfer -- Environmental Microbiology -- Life Support Systems -- Population Dynamics -- Yeasts
Аннотация: Closed Artificial ecosystems (CAES) have good prospects for wide use as new means for quantitative studies of different types of both natural ecosystems and man-made ones. The paper deals with the discussion of three points of CAES applications. The first one is of importance for theoretical ecology development and is connected with bringing together В«holisticВ» and В«merologicalВ» approaches in ecosystems studies. Using CAES, we can combine both approaches, taking into account the biotic turnover of limiting substrates which few in number even for complicated natural ecosystems. The second CAES use concerns the development of В«ecosystems healthВ» concept and application of a key-factor-approach for the indication and measurement of healthy unhealthy state and functioning of ecosystems or their links. The third use is more of an applied nature, oriented to the intensification of bioremediation or biodepollution processes in different types of ecosystems, including the global biosphere. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, 660036, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pechurkin, N.S.; Shirobokova, I.M.

Найти похожие

12.

Material balance and diet in bioregenerative life support systems: Connection with coefficient of closure / N. S. Manukovsky [et al.] // Advances in Space Research. - 2005. - Vol. 35, Is. 9 SPEC. ISS. - P1563-1569, DOI 10.1016/j.asr.2005.01.002 . - ISSN 0273-1177
Кл.слова (ненормированные):
Bioregenerative life support system -- Coefficient of closure -- Diet -- Soil-like substrate -- Additives -- Degradation -- Materials balance -- Nitrogen -- Nutrition -- Stoichiometry -- Bioregenerative life support systems -- Coefficient of closure -- Soil-like substrates -- Life support systems (spacecraft) -- vegetable protein -- biomass -- conference paper -- culture medium -- diet -- feces -- growth, development and aging -- human -- methodology -- microclimate -- plant -- urine -- waste management -- Biomass -- Culture Media -- Diet -- Ecological Systems, Closed -- Feces -- Humans -- Life Support Systems -- Plant Proteins -- Plants, Edible -- Urine -- Waste Management
Аннотация: Bioregenerative life support systems (BLSS) with different coefficients of closure are considered. The 66.2% coefficient of closure achieved in "BIOS-3" facility experiments has been taken as a base value. The increase in coefficient of closure up to 72.6-93.0% is planned due to use of soil-like substrate (SLS) and concentrating of urine. Food values were estimated both in a base variant ("BIOS-3"), and with increases in the coefficient of closure. It is shown that food requirements will be more fully satisfied by internal crop production with an increase in the coefficient of closure of the BLSS. Changes of massflow rates on an 'input-output' and inside BLSS are considered. Equations of synthesis and degradation of organic substances in BLSS were examined using a stoichiometric model. The paper shows that at incomplete closure of BLSS containing SLS there is a problem of nitrogen balancing. To compensate for the removal of nitrogen from the system in urine and feces, it is necessary to introduce food and a nitrogen-containing additive. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Academgorodok, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Manukovsky, N.S.; Kovalev, V.S.; Somova, L.A.; Gurevich, Yu.L.; Sadovsky, M.G.

Найти похожие

13.

Interaction of a mixed yeast culture in an "autotroph-heterotroph" system with a closed atmosphere cycle and spatially separated components / T. I. Pisman, L. A. Somova // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1751-1756, DOI 10.1016/S0273-1177(03)00116-9 . - ISSN 0273-1177
Кл.слова (ненормированные):
Metabolism -- Oxygen -- Substrates -- Yeast -- Closed atmosphere cycle -- Space research -- carbon dioxide -- oxygen -- biosphere -- anaerobic growth -- article -- biomass -- Candida -- Chlorella -- comparative study -- culture medium -- ecosystem -- growth, development and aging -- metabolism -- microclimate -- Anaerobiosis -- Biomass -- Candida -- Carbon Dioxide -- Chlorella -- Culture Media -- Ecological Systems, Closed -- Ecosystem -- Environment, Controlled -- Oxygen
Аннотация: The study considers an experimental model of the "autotroph-heterotroph" system with a closed atmosphere cycle, in which the heterotrophic link is a mixed yeast population. The autotrophic link is represented by the algae Chlorella vulgaris and the heterotrophic link by the yeasts Candida utilis and Candida guilliermondii. The controls are populations of Chlorella and the same yeasts isolated from the atmosphere. It has been shown that the outcome of competition in the heterotrophic link depends on the strategy of the yeast population towards the substrate and oxygen. The C. utilis population quickly utilizes the substrate as it is an r-strategist and is less sensitive to oxygen deficiency. The C. guilliermondii population consumes low concentrations of the substrate because it is a K-strategist, but it is more sensitive to oxygen deficiency. That is why, in the "autotroph-heterotroph" system with a closed gas cycle, after a considerable amount of the substrate has been consumed, the C. guilliermondii population becomes more competitive that the C. utilis population. In the culture of yeasts, isolated from the atmosphere, the C. utilis population finds itself in more favorable conditions due to oxygen deficiency. The system with a complex heterotrophic component survive longer than a system whose heterotrophic component is represented by only one yeast species. This is explained for by the positive metabolite interaction of yeasts and a more complete utilization of the substrate by a mixed culture of yeasts featuring different strategies towards the substrate. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Inst. of Biophysics Siberian Branch, Russian Academy Science, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pisman, T.I.; Somova, L.A.

Найти похожие

14.

Microbial sulfate reduction in a brackish meromictic steppe lake / D. B. Kosolapov [et al.] // Aquatic Ecology. - 2003. - Vol. 37, Is. 3. - P215-226, DOI 10.1023/A:1025871300917 . - ISSN 1386-2588
Кл.слова (ненормированные):
Anoxic waters -- Brackish meromictic lake -- Littoral and profundal sediments -- Sulfate reduction -- anoxic conditions -- brackish water -- meromictic lake -- reduction -- sulfate -- Russian Federation -- Viscum -- Viscum album
Аннотация: Patterns of sulfate reduction were studied in water and sediments of Lake Shira, South Siberia, Russia. The lake was characterized by a high level of sulfate (91-116 mM). The concentration of hydrogen sulfide in the anoxic waters of the lake reached 0.6 mM. In summer the sulfate reduction rate in the water column, measured by radiometric technique, varied from 0.25 to 9.81 ?mol sulfate l-1 d-1. There were two peaks of sulfate reduction activity: just below the chemocline and near the sediment surface. Sulfate reduction rate in the profundal silts ranged from 4.1 to 90.6 ?mol l-1 d-1. The zone of the most active sulfate reduction was restricted to the surface sediment layers. The acceleration of sulfate reduction rate (up to 236 ?mol l-1 d-1) and the increase of density of viable sulfate reducers (up to 2 x 105 cells ml-1) were recorded in the littoral sediments adjacent to the mouth of the Son River and sewage discharge. It was apparently caused by the input of allochthonous organic substrates and also by a high environmental temperature. On an areal basis, sulfate reduction rate in the water was approximately 8 times higher than that in the profundal sediments. Sulfate reduction was the most important process of anaerobic oxidation of organic carbon in Lake Shira. In summer in the profundal zone of the lake, sulfate reducers were able to mineralize about 67% of the daily integrated primary production of phototrophic and chemotrophic organisms.

Scopus
Держатели документа:
Inst. for Biology of Inland Waters, Russian Academy of Sciences, Borok, Nekouz, Yaroslavl, 152742, Russian Federation
Inst. Biophys. Siberian Br. Russ. A., Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kosolapov, D.B.; Rogozin, D.Yu.; Gladchenko, I.A.; Kopylov, A.I.; Zakharova, E.E.

Найти похожие

15.

A NEW EXPERIMENTAL APPROACH TO THE SEARCH FOR CHEMICAL DENSITY FACTORS IN THE REGULATION OF MONOCULTURE GROWTH [Text] / A. G. DEGERMENDZHY, V. V. ADAMOVICH, V. A. ADAMOVICH // J. Gen. Microbiol. - 1993. - Vol. 139. - P2027-2031. - Cited References: 7 . - 5. - ISSN 0022-1287

РУБ Microbiology

Аннотация: In monocultures of micro-organisms, growth is controlled by feedback mechanisms involving chemical factors such as limiting substrates and inhibitory metabolic products. The role of such feedback in the growth regulation of Escherichia coli O-124 was investigated by growing cells in batch culture using a medium containing glucose and mineral salts. In various phases of growth, portions of the native culture were diluted with culture filtrate, so that although cell density decreased, the chemical composition of the growth medium was unaltered. As the diluted cultures grew, variations in growth acceleration were calculated and compared with those of native (undiluted) cultures. Towards the end of the exponential phase and in the growth deceleration phase, the specific feedback level (FBL) was between -20 and -200 (h g l-1)-1. The feedback components resulting from changes in glucose concentration were calculated using experimentally determined values of mu(max) (0.55 +/- 0.05 h-1) and K(s) (2.5 +/- 0.7 mg l-1). Only 0.1-40% of FBL could be accounted for by changes in glucose concentration, indicating the presence of additional growth regulators. The method developed may become a new tool for determination of growth-regulating cell-density factors in microbial cultures.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
DEGERMENDZHY, A.G.; ADAMOVICH, V.V.; ADAMOVICH, V.A.

Найти похожие

16.

On the cooperative effect of organic and mineral substrates on growth rates of symbionts in the symbiotic complex Paramecium bursaria-Zoochlorella / Yu. V. Barkhatov, V. G. Gubanov, V. E. Sazonova // Russian Journal of Ecology. - 1998. - Vol. 29, Is. 4. - P287-289 . - ISSN 1067-4136

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Barkhatov, Yu.V.; Gubanov, V.G.; Sazonova, V.E.

Найти похожие

17.

A QUANTUM CHEMICAL STUDY OF THE FORMATION OF 2-HYDROPEROXY-COELENTERAZINE IN THE Ca2+-REGULATED PHOTOPROTEIN OBELIN [Text] / L. Y. Antipina [et al.] // J. Struct. Chem. - 2011. - Vol. 52, Is. 5. - P870-875. - Cited References: 19. - The work was supported by RFBR (07-04-00930-a), the "Molecular and Cell Biology" Program of the Presidium of the Russian Academy of Sciences, and the Program of the Siberian Division of the Russian Academy of Sciences (project No. 2) within the implementation of the Federal Targeted Program "Scientific and Scientific Pedagogical Personnel of Innovative Russia, 2010" (P333 and P213). . - ISSN 0022-4766

РУБ Chemistry, Inorganic & Nuclear + Chemistry, Physical
Рубрики:
CALCIUM-DISCHARGED OBELIN
   SEMIEMPIRICAL METHODS
   1.7 ANGSTROM
   OPTIMIZATION
   PARAMETERS
   MECHANISM
   FLUORESCENCE
   ELEMENTS
   PROTEIN
   EMITTER
Кл.слова (ненормированные):
coelenterazine -- 2-hydroperoxy-coelenterazine -- Obelia longissima -- Renilla muelleri
Аннотация: The Ca2+-regulated photoprotein obelin determines the luminescence of the marine hydroid Obelia longissima. Bioluminescence is initiated by calcium and appears as a result of the oxidative decarboxylation related to the coelenterazine substrate. The luciferase of the luminescent marine coral Renilla muelleri (RM) also uses coelenterazine as a substrate. However, three proteins are involved in the in vivo bioluminescence of these animals: luciferase, green fluorescent protein, and Ca2+-regulated coelenterazine-binding protein (CBP). In fact, CBP that contains one strongly bound coelenterazine molecule is the RM luciferase substrate in the in vivo bioluminescent reaction. Coelenterazine becomes available for oxygen and the reaction with luciferase only after binding CBP with calcium ions. Unlike Ca2+-regulated photoproteins, the coelenterazine molecule is not activated by oxygen in the CBP molecule. In this work, by means of quantum chemical methods the behavior of substrates in these proteins is analyzed. It is shown that coelenterazine can form different tautomers: CLZ(2H) and CLZ(7H). The formation of 2-hydroperoxy-coelenterazine is studied. According to the obtained data, these proteins use different forms of the substrates for the reaction. In obelin, the substrate is in the CLZ(2H) form that affords hydrogen peroxide. In RM, coelenterazine is in the CLZ(7H) form, and therefore, CBP is not activated by oxygen.

Держатели документа:
[Antipina, L. Yu
Tomilin, F. N.
Ovchinnikov, S. G.] Russian Acad Sci, LV Kirensky Phys Inst, Siberian Div, Krasnoyarsk, Russia
[Vysotskii, E. S.] Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk, Russia
[Antipina, L. Yu
Ovchinnikov, S. G.] MF Reshetnev Siberian State Aerosp Univ, Krasnoyarsk, Russia
ИФ СО РАН
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Antipina, L.Y.; Tomilin, F.N.; Vysotskii, E.S.; Ovchinnikov, S.G.

Найти похожие

18.

PROSPECTS FOR APPLICATION OF BIOLUMINESCENCE METHOD IN MEDICINE [Текст] / I. I. GITELZON, T. P. SANDALOVA // VESTNIK AKADEMII MEDITSINSKIKH NAUK SSSR. - 1990. - Is. 9. - С. 31-35. - Cited References: 41 . - ISSN 0002-3027

РУБ Medicine, General & Internal
Рубрики:
AMINO-ACID SEQUENCE
   NUCLEOTIDE-SEQUENCE
   VIBRIO-HARVEYI
   BACTERIAL LUCIFERASE
   FIREFLY LUCIFERASE
   SUBUNIT
   CELLS
   GENE
   PHOTOPROTEINS
   EXPRESSION
Аннотация: Major advances in the development and application of the bioluminescent analysis to detect certain biologically active substances are discussed. The main merit of the method lies in its high sensitivity and specificity along with its simplicity and rapid performance. The available methodologies allow for detection of substances of varying nature: Ca2+, ATP, FMN, NAD(P), long-chain aldehydes, ATP- and NAD(P)-dependent enzymes and their substrates, many xenobiotics and antibiotics, and mutagens. The bioluminescence methodologies may be widely applied in clinical laboratory diagnosis.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
GITELZON, I.I.; SANDALOVA, T.P.

Найти похожие

19.

A glucose-utilizing strain, cupriavidus euthrophus B-10646: Growth kinetics, characterization and synthesis of multicomponent PHAs / T. Volova [et al.] // PLoS ONE. - 2014. - Vol. 9, Is. 2, DOI 10.1371/journal.pone.0087551 . - ISSN 1932-6203
Кл.слова (ненормированные):
3 hydroxybutyrate 3 hydroxyhexanoate 3 hydroxyvalerate copolymer -- 3 hydroxybutyrate 4 hydroxybutyrate 3 hydroxyvalerate copolymer -- copolymer -- gamma butyrolactone -- glucose -- hexanoic acid -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- polystyrene -- propionic acid -- unclassified drug -- valeric acid -- animal cell -- article -- bacterial growth -- bacterium culture -- cell adhesion -- cell proliferation -- crystal structure -- culture optimization -- Cupriavidus -- Cupriavidus euthrophus -- decomposition -- elasticity -- film -- glucose utilization -- kinetics -- mechanics -- melting point -- mouse -- nonhuman -- nucleotide sequence -- physical chemistry -- polymerization -- strength -- synthesis
Аннотация: This study investigates kinetic and production parameters of a glucose-utilizing bacterial strain, C. eutrophus B-10646, and its ability to synthesize PHA terpolymers. Optimization of a number of parameters of bacterial culture (cell concentration in the inoculum, physiological activity of the inoculum, determined by the initial intracellular polymer content, and glucose concentration in the culture medium during cultivation) provided cell concentrations and PHA yields reaching 110 g/L and 80%, respectively, under two-stage batch culture conditions. Addition of precursor substrates (valerate, hexanoate, propionate, ?-butyrolactone) to the culture medium enabled synthesis of PHA terpolymers, P(3HB/3HV/4HB) and P(3HB/ 3HV/3HHx), with different composition and different molar fractions of 3HB, 3HV, 4HB, and 3HHx. Different types of PHA terpolymers synthesized by C. eutrophus B-10646 were used to prepare films, whose physicochemical and physical-mechanical properties were investigated. The properties of PHA terpolymers were significantly different from those of the P3HB homopolymer: they had much lower degrees of crystallinity and lower melting points and thermal decomposition temperatures, with the difference between these temperatures remaining practically unchanged. Films prepared from all PHA terpolymers had higher mechanical strength and elasticity than P3HB films. In spite of dissimilar surface structures, all films prepared from PHA terpolymers facilitated attachment and proliferation of mouse fibroblast NIH 3T3 cells more effectively than polystyrene and the highly crystalline P3HB. Copyright: © 2014 Volova et al.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Science, Moscow, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.; Kiselev, E.; Vinogradova, O.; Nikolaeva, E.; Chistyakov, A.; Sukovatiy, A.; Shishatskaya, E.

Найти похожие

20.

A Glucose-Utilizing Strain, Cupriavidus euthrophus B-10646: Growth Kinetics, Characterization and Synthesis of Multicomponent PHAs [Text] / T. . Volova [et al.] // PLoS One. - 2014. - Vol. 9, Is. 2. - Ст. e87551, DOI 10.1371/journal.pone.0087551. - Cited References: 64. - This study was financially supported by Project "Biotechnologies of novel biomaterials: Innovative Biopolymers and Biomedicine Devices" (Agreement No. 11.G34.31.0013 with Amendment No. 1 of 15 February 2013) in accordance with Resolution No. 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning." The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. . - ISSN 1932-6203

РУБ Multidisciplinary Sciences
Рубрики:
RALSTONIA-EUTROPHA
   BIODEGRADABLE POLYHYDROXYALKANOATES
   AEROMONAS-HYDROPHILA
   ESCHERICHIA-COLI
   MOLECULAR-WEIGHT
   SURFACE-ENERGY
   NORTH PACIFIC
   TERPOLYESTER
   BIOSYNTHESIS
   POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE-CO-3-HYDROXYHEXANOATE)
Аннотация: This study investigates kinetic and production parameters of a glucose-utilizing bacterial strain, C. eutrophus B-10646, and its ability to synthesize PHA terpolymers. Optimization of a number of parameters of bacterial culture (cell concentration in the inoculum, physiological activity of the inoculum, determined by the initial intracellular polymer content, and glucose concentration in the culture medium during cultivation) provided cell concentrations and PHA yields reaching 110 g/L and 80%, respectively, under two-stage batch culture conditions. Addition of precursor substrates (valerate, hexanoate, propionate, c-butyrolactone) to the culture medium enabled synthesis of PHA terpolymers, P(3HB/3HV/4HB) and P(3HB/3HV/3HHx), with different composition and different molar fractions of 3HB, 3HV, 4HB, and 3HHx. Different types of PHA terpolymers synthesized by C. eutrophus B-10646 were used to prepare films, whose physicochemical and physicalmechanical properties were investigated. The properties of PHA terpolymers were significantly different from those of the P3HB homopolymer: they had much lower degrees of crystallinity and lower melting points and thermal decomposition temperatures, with the difference between these temperatures remaining practically unchanged. Films prepared from all PHA terpolymers had higher mechanical strength and elasticity than P3HB films. In spite of dissimilar surface structures, all films prepared from PHA terpolymers facilitated attachment and proliferation of mouse fibroblast NIH 3T3 cells more effectively than polystyrene and the highly crystalline P3HB.

WOS
Держатели документа:
[Volova, Tatiana
Kiselev, Evgeniy
Nikolaeva, Elena
Sukovatiy, Aleksey
Shishatskaya, Ekaterina] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia
[Volova, Tatiana
Vinogradova, Olga
Shishatskaya, Ekaterina] Siberian Fed Univ, Krasnoyarsk, Russia
[Chistyakov, Anton] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T...; Kiselev, E...; Vinogradova, O...; Nikolaeva, E...; Chistyakov, A...; Sukovatiy, A...; Shishatskaya, E...; Project "Biotechnologies of novel biomaterials: Innovative Biopolymers and Biomedicine Devices" [11.G34.31.0013]

Найти похожие