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Tritium in water bodies of the Yenisei Basin in the impact zone of the mining-and-chemical plant of the Ministry of the Nuclear Power Industry of the Russian Federation / A. Ya. Bolsunovskii, L. G. Bondareva // Russian Journal of Ecology. - 2005. - Vol. 36, Is. 1. - P52-56, DOI 10.1007/s11184-005-0009-y . - ISSN 1067-4136
Кл.слова (ненормированные):
Pore water -- The Integrated Mining Chemical Plant -- The Yenisei -- Tritium -- tritium -- Tritium

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovskii, A.Ya.; Bondareva, L.G.

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Tritium in surface waters of the Yenisei River basin / A. Y. Bolsunovsky, L. G. Bondareva // Journal of Environmental Radioactivity. - 2003. - Vol. 66, Is. 3. - P285-294, DOI 10.1016/S0265-931X(02)00132-7 . - ISSN 0265-931X
Кл.слова (ненормированные):
Deep-well injection of radionuclides -- Nuclear reactor -- Surface and ground waters -- Tritium -- Yenisei River basin -- Cooling water -- Nuclear reactors -- Sediments -- Surface waters -- River basins -- Tritium -- carbon 14 -- surface water -- tritium -- groundwater -- nuclear power plant -- radioactive pollution -- surface water -- tritium -- article -- catchment -- measurement -- radioactive contamination -- river -- sediment -- Environmental Monitoring -- Geologic Sediments -- Mining -- Power Plants -- Reference Values -- Russia -- Tritium -- Water Pollutants, Radioactive -- Russian Federation -- Tritium
Аннотация: This paper reports an investigation of the tritium content in the surface waters of the Yenisei River basin near the Mining and Chemical Combine (MCC). In 2001 the maximum tritium concentration in the Yenisei River did not exceed 4 В± 1 Bq l-1, which is consistent with the data of 1998-99. However, it has been found that there are surface waters containing enhanced tritium as compared with the background values for the Yenisei River. For instance, in the Ploskii Stream and the Shumikha River the maximum tritium concentrations amount to 168 and 81 Bq l-1, respectively. The source of tritium in these surface waters is the last operating reactor at the MCC, which still uses the Yenisei water as coolant. In water and sediment samples of the Bolshaya Tel River (a tributary of the Yenisei River) the tritium content turned out to be at least 10 times higher than the background values for the Yenisei River. The measurements conducted at the RPA RADON (Moscow) revealed not only tritium but also the artificial radionuclide 14C in the Bolshaya Tel samples. The data obtained suggest that the Bolshaya Tel River receives the major part of tritium from sediments rather than from the water catchment area. This allows the conclusion that there is water exchange between the surface waters and the radioactively contaminated underground horizons of the "Severny" testing site. В© 2002 Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.Y.; Bondareva, L.G.

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New data on the content of tritium in a tributary of the Yenisei River. / A. Y. Bolsunovsky, L. G. Bondareva // Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections / translated from Russian. - 2002. - Vol. 385. - P380-383 . - ISSN 0012-4966
Кл.слова (ненормированные):
carbon -- fresh water -- tritium -- article -- geography -- Russian Federation -- water pollutant -- Carbon Radioisotopes -- Fresh Water -- Geography -- Russia -- Tritium -- Water Pollutants, Radioactive

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.Y.; Bondareva, L.G.

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Effect of tritium on growth and lumuninescence of P. phosporeum [Text] / M. . Alexandrova [et al.] // Luminescence. - 2010. - Vol. 25, Is. 2. - P125-126. - Cited References: 3 . - 2. - ISSN 1522-7235

РУБ Biochemistry & Molecular Biology

Держатели документа:
[Alexandrova, M.
Rozhko, T.
Bondareva, L.
Kudryasheva, N.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
[Bondareva, L.
Vydryakova, G.
Bolsunovsky, A.
Kudryasheva, N.] RAS, Inst Biophys, SB, Krasnoyarsk 660036, Russia
[Badun, G.] Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119991, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
Alexandrova, M...; Rozhko, T...; Badun, G...; Bondareva, L...; Vydryakova, G...; Bolsunovsky, A...; Kudryasheva, N...

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    Effect of radioisotope tritium on bioluminescence and mutations in luminous bacteria P. phosphoreum 1883 IBSO [Text] / O. . Guseynov [et al.] // Luminescence. - 2014. - Vol. 29. - P77-78. - Cited References: 6 . - ISSN 1522-7235. - ISSN 1522-7243
Рубрики:
RIBOSOMAL-RNA
   FRAGMENT
   AM-241

WOS
Держатели документа:
[Guseynov, Oleg
Litvinova, Irina
Karpenok, Polina
Guseynova, Valeriya
Petrova, Alena
Kudryasheva, Nadezhda] Siberian Fed Univ, Krasnoyarsk, Russia
[Selivanova, Maria
Kudryasheva, Nadezhda] Inst Biophys SB RAS, Krasnoyarsk, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Guseynov, O...; Selivanova, M...; Litvinova, I...; Karpenok, P...; Guseynova, V...; Petrova, A...; Kudryasheva, N...

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Comparison of chronic low-dose effects of alpha- and beta-emitting radionuclides on marine bacteria / M. A. Selivanova [et al.] // Cent. Eur. J. Biol. - 2014. - Vol. 9, Is. 10. - P951-959, DOI 10.2478/s11535-014-0331-0 . - ISSN 1644-3632
Кл.слова (ненормированные):
Am-241 -- Hormesis -- Luminous bacteria -- Peroxides -- Radiotoxicity -- Tritium
Аннотация: Effects of Americium-241 (241Am), alpha-emitting radionuclide of high specific radioactivity, and tritium (3H), beta-emitting radionuclide, on luminous bacteria Photobacterium phosphoreum were compared. Bioluminescence intensity served as a marker of bacterial physiological activity. Three successive stages in the bioluminescence response to 241Am and 3H were found under conditions of lowdose irradiation: (1) absence of effects, (2) activation, and (3) inhibition. They were interpreted in terms of bacterial response to stressfactor as stress recognition, adaptive response/syndrome, and suppression of physiological function (i.e. radiation toxicity). Times of bioluminescence activation (TBA) and inhibition (TBI) were suggested as parameters to characterize hormesis and toxic stages in a course of chronic low-dose irradiation of the microorganisms. Values of TBA and TBI of 241Am were shorter than those of 3H, revealing higher impact of alpha-irradiation (as compared to beta-irradiation) under comparable radiation doses. Increases of peroxide concentration and NADH oxidation rates in 241Am aquatic solutions were demonstrated; these were not found in tritiated water. The results reveal a biological role of reactive oxygen species generated in water solutions as secondary products of the radioactive decay. The study provides a scientific basis for elaboration of bioluminescence-based assay to monitor radiotoxicity of alpha- and beta-emitting radionuclides in aquatic solutions. © 2014 Versita Warsaw and Springer-Verlag Wien.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, 660036 Krasnoyarsk, Russian Federation
Siberian Federal University, 660041 Krasnoyarsk, Russian Federation
Siberian State Technological University, Lesosibirsk, Krasnoyarsk region, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Selivanova, M.A.; Rozhko, T.V.; Devyatlovskaya, A.N.; Kudryasheva, N.S.

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Tritium in surface waters of the Yenisei River basin [Text] / A. Y. Bolsunovsky, L. G. Bondareva // J. Environ. Radioact. - 2003. - Vol. 66, Is. 3. - P. 285-294, DOI 10.1016/S0265-931X(02)00132-7. - Cited References: 11 . - ISSN 0265-931X

РУБ Environmental Sciences

Кл.слова (ненормированные):
tritium -- Yenisei River basin -- surface and ground waters -- nuclear reactor -- deep-well injection of radionuclides
Аннотация: This paper reports an investigation of the tritium content in the surface waters of the Yenisei River basin near the Mining and Chemical Combine (MCC). In 2001 the maximum tritium concentration in the Yenisei River did not exceed 4 +/- 1 Bq l(-1), which is consistent with the data of 1998-99. However, it has been found that there are surface waters containing enhanced tritium as compared with the background values for the Yenisei River. For instance, in the Ploskii Stream and the Shumikha River the maximum tritium concentrations amount to 168 and 81 Bq l(-1), respectively. The source of tritium in these surface waters is the last operating reactor at the MCC, which still uses the Yenisei water as coolant. In water and sediment samples of the Bolshaya Tel River (a tributary of the Yenisei River) the tritium content turned out to be at least 10 times higher than the background values for the Yenisei River. The measurements conducted at the RPA RADON (Moscow) revealed not only tritium but also the artificial radionuclide C-14 in the Bolshaya Tel samples. The data obtained suggest that the Bolshaya Tel River receives the major part of tritium from sediments rather than from the water catchment area. This allows the conclusion that there is water exchange between the surface waters and the radioactively contaminated underground horizons of the "Severny" testing site. (C) 2002 Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
RAS, SB, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bolsunovsky, A.Y.; Bondareva, L.G.

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Effect of low-dose ionizing radiation on luminous marine bacteria: radiation hormesis and toxicity [Text] / N. S. Kudryasheva, T. V. Rozhko // J. Environ. Radioact. - 2015. - Vol. 142. - P68-77, DOI 10.1016/j.jenvrad.2015.01.012. - Cited References:131. - This work was supported by the Russian Foundation for Basic Research, Grant No.13-04-01305a, the Program "Molecular and Cellular Biology" of the Russian Academy of Sciences, project VI 57.1.1. The part of the work (review of effects of americium-241) was supported by the Russian Science Foundation, Grant No. 14-14-00076. . - ISSN 0265-931X. - ISSN 1879-1700

РУБ Environmental Sciences
Рубрики:
RECOMBINANT LUMINESCENT MICROORGANISMS
PHOTOBACTERIUM-LEIOGNATHI
Кл.слова (ненормированные):
Marine bacteria -- Low-dose effects -- Radiation hormesis -- Radiotoxicity -- Reactive oxygen species
Аннотация: The paper summarizes studies of effects of alpha- and beta-emitting radionuclides (americium-241, uranium-235+238, and tritium) on marine microorganisms under conditions of chronic low-dose irradiation in aqueous media. Luminous marine bacteria were chosen as an example of these microorganisms; bioluminescent intensity was used as a tested physiological parameter. Non-linear dose-effect dependence was demonstrated. Three successive stages in the bioluminescent response to americium-241 and tritium were found: 1 - absence of effects (stress recognition), 2 - activation (adaptive response), and 3 - inhibition (suppression of physiological function, i.e. radiation toxicity). The effects were attributed to radiation hormesis phenomenon. Biological role of reactive oxygen species, secondary products of the radioactive decay, is discussed. The study suggests an approach to evaluation of non-toxic and toxic stages under conditions of chronic radioactive exposure. (C) 2015 Elsevier Ltd. All rights reserved.

WOS
Держатели документа:
Inst Biophys SB RAS, Krasnoyarsk 660036, Russia
Siberian Fed Univ, Krasnoyarsk 660041, Russia
Krasnoyarsk State Med Acad, Krasnoyarsk 660022, Russia

Доп.точки доступа:
Kudryasheva, N. S.; Rozhko, T. V.; Russian Foundation for Basic Research [13-04-01305a]; Program "Molecular and Cellular Biology" of the Russian Academy of Sciences [VI 57.1.1]; Russian Science Foundation [14-14-00076]

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On the mechanism of biological activation by tritium / T. V. Rozhko [et al.] // J. Environ. Radioact. - 2016. - Vol. 157. - P131-135, DOI 10.1016/j.jenvrad.2016.03.017 . - ISSN 0265-931X
Кл.слова (ненормированные):
DNA mutations -- Low-dose effect -- Luminous marine bacteria -- Radiation hormesis -- Tritium
Аннотация: The mechanism of biological activation by beta-emitting radionuclide tritium was studied. Luminous marine bacteria were used as a bioassay to monitor the biological effect of tritium with luminescence intensity as the physiological parameter tested. Two different types of tritium sources were used: HTO molecules distributed regularly in the surrounding aqueous medium, and a solid source with tritium atoms fixed on its surface (tritium-labeled films, 0.11, 0.28, 0.91, and 2.36 MBq/cm2). When using the tritium-labeled films, tritium penetration into the cells was prevented. The both types of tritium sources revealed similar changes in the bacterial luminescence kinetics: a delay period followed by bioluminescence activation. No monotonic dependences of bioluminescence activation efficiency on specific radioactivities of the films were found. A 15-day exposure to tritiated water (100 MBq/L) did not reveal mutations in bacterial DNA. The results obtained give preference to a "non-genomic" mechanism of bioluminescence activation by tritium. An activation of the intracellular bioluminescence process develops without penetration of tritium atoms into the cells and can be caused by intensification of trans-membrane cellular processes stimulated by ionization and radiolysis of aqueous media. © 2016 Elsevier Ltd.

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Смотреть статью
Держатели документа:
Krasnoyarsk State Medical Academy, P.Zheleznyaka 1, Krasnoyarsk, Russian Federation
Siberian Federal University, Svobodny 79, Krasnoyarsk, Russian Federation
Moscow State University, Department of Chemistry, Moscow, Russian Federation
Institute of Biophysics SB RAS, Akademgorodok 50/50, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Rozhko, T. V.; Badun, G. A.; Razzhivina, I. A.; Guseynov, O. A.; Guseynova, V. E.; Kudryasheva, N. S.

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10.

Reactive Oxygen Species and low-dose effects of tritium on bacterial cells / T. V. Rozhko [et al.] // J. Environ. Radioact. - 2019. - Vol. 208-209. - Ст. 106035, DOI 10.1016/j.jenvrad.2019.106035 . - ISSN 0265-931X
Кл.слова (ненормированные):
Bystander effect -- Low-dose effect -- Luminous marine bacterium -- Radiation hormesis -- Reactive oxygen species -- Signaling molecules -- Tritium -- Bioluminescence -- Cell signaling -- Cells -- Cytology -- Irradiation -- Oxygen -- Phosphorescence -- Physiological models -- Tritium -- Bystander effects -- Low dose effects -- Marine bacterium -- Radiation hormesis -- Reactive oxygen species -- Signaling molecules -- Bacteria -- Bacteria (microorganisms)
Аннотация: The paper continues study of exposures of luminous marine bacteria to low-dose radiation of tritium; tritiated water (HTO) was applied as a source of the irradiation. Hypothesis on involvement of Reactive Oxygen Species (ROS) to signaling mechanism of bacterial cells under exposure to low-intensity tritium radiation was verified. Bacterial bioluminescence intensity was considered as a tested physiological parameter; it was compared to the ROS production in the bacterial environment of different activity concentrations: 0.03, 4.0, and 500 MBq/L. Exposure of the bacteria to chronic low-dose tritium irradiation (<0.08 Gy) increased bioluminescence intensity and ROS production considerably (up to 300%). Spearman rank correlation coefficients were calculated and confirmed relations between the bioluminescence intensity and ROS production. Additional peculiarities of HTO effect were: independence of the bioluminescence intensity and ROS content on HTO activity concentration; low ROS content in bacteria-free aquatic environment. Effects of HTO on bacterial bioluminescence were attributed to: (1) trigger function of tritium decay products in the bacterial metabolic oxygen-dependent processes, with bioluminescence involved; (2) signaling role of ROS as intercellular messengers in “bystander effect”; (3) fixed amount of bacterial cells (3•107 cells/mL) provided the upper limits of the bioluminescence intensity and ROS content. As an outlook, in spite of low energy of tritium decay, its influence on aquatic biota via ROS production by microorganisms should be taken into consideration. © 2019 Elsevier Ltd

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Смотреть статью,
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Держатели документа:
Krasnoyarsk State Medical University, P.Zheleznyaka 1, Krasnoyarsk, 660022, Russian Federation
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation
Moscow State University, Department of Chemistry, Moscow119991, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Rozhko, T. V.; Nogovitsyna, E. I.; Badun, G. A.; Lukyanchuk, A. N.; Kudryasheva, N. S.

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11.

Humic substances mitigate the impact of tritium on luminous marine bacteria. Involvement of reactive oxygen species / T. V. Rozhko, O. V. Kolesnik, G. A. Badun [et al.] // International Journal of Molecular Sciences. - 2020. - Vol. 21, Is. 18. - Ст. 6783. - P1-12, DOI 10.3390/ijms21186783 . - ISSN 1661-6596
Кл.слова (ненормированные):
Adaptive response -- Bioassay -- Detoxification -- Hormesis -- Humic substances -- Luminous marine bacterium -- Reactive oxygen species -- Toxicity -- Tritium
Аннотация: The paper studies the combined effects of beta-emitting radionuclide tritium and Humic Substances (HS) on the marine unicellular microorganism—luminous bacteria—under conditions of low-dose radiation exposures (<0.04 Gy). Tritium was used as a component of tritiated water. Bacterial luminescence intensity was considered as a tested physiological parameter. The bioluminescence response of the marine bacteria to tritium corresponded to the “hormesis” model: it included stages of bioluminescence inhibition and activation, as well as the absence of the effect. HS were shown to decrease the inhibition and activation effects of tritium, similar to those of americium-241, alpha-emitting radionuclide, studied earlier. Correlations between the bioluminescence intensity and the content of Reactive Oxygen Species (ROS) were found in the radioactive bacterial suspensions. The results demonstrate an important role of HS in natural processes in the regions of low radioactive contamination: HS can mitigate radiotoxic effects and adaptive response of microorganisms to low-dose radioactive exposures. The involvement of ROS in these processes was demonstrated. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Scopus
Держатели документа:
Krasnoyarsk State Medical Academy, Krasnoyarsk, 660022, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center ‘Krasnoyarsk Science Center SB RAS’, Krasnoyarsk, 660036, Russian Federation
Department of Chemistry, Moscow State University, Moscow, 119991, Russian Federation
Biology Department, Irkutsk State University, Irkutsk, 664003, Russian Federation
Biophysics Department, Siberian Federal University, Svobodny 79, Krasnoyarsk, 660041, Russian Federation

Доп.точки доступа:
Rozhko, T. V.; Kolesnik, O. V.; Badun, G. A.; Stom, D. I.; Kudryasheva, N. S.

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12.

Humic Substances Mitigate the Impact of Tritium on Luminous Marine Bacteria. Involvement of Reactive Oxygen Species / T. V. Rozhko, O. V. Kolesnik, G. A. Badun [et al.] // Int. J. Mol. Sci. - 2020. - Vol. 21, Is. 18. - Ст. 6783, DOI 10.3390/ijms21186783. - Cited References:74. - This work was supported by RFBR-Krasnoyarsk Regional Foundation N 18-44-242002, 18-44-240004. . - ISSN 1422-0067

РУБ Biochemistry & Molecular Biology + Chemistry, Multidisciplinary
Рубрики:
IONIZING-RADIATION
OXIDATIVE STRESS
DETOXIFICATION PROCESSES
Кл.слова (ненормированные):
tritium -- humic substances -- luminous marine bacterium -- bioassay -- detoxification -- reactive oxygen species -- toxicity -- adaptive response -- hormesis
Аннотация: The paper studies the combined effects of beta-emitting radionuclide tritium and Humic Substances (HS) on the marine unicellular microorganism-luminous bacteria-under conditions of low-dose radiation exposures (<0.04 Gy). Tritium was used as a component of tritiated water. Bacterial luminescence intensity was considered as a tested physiological parameter. The bioluminescence response of the marine bacteria to tritium corresponded to the "hormesis" model: it included stages of bioluminescence inhibition and activation, as well as the absence of the effect. HS were shown to decrease the inhibition and activation effects of tritium, similar to those of americium-241, alpha-emitting radionuclide, studied earlier. Correlations between the bioluminescence intensity and the content of Reactive Oxygen Species (ROS) were found in the radioactive bacterial suspensions. The results demonstrate an important role of HS in natural processes in the regions of low radioactive contamination: HS can mitigate radiotoxic effects and adaptive response of microorganisms to low-dose radioactive exposures. The involvement of ROS in these processes was demonstrated.

WOS
Держатели документа:
Krasnoyarsk State Med Acad, Krasnoyarsk 660022, Russia.
RAS, Inst Biophys, Fed Res Ctr Krasnoyarsk Sci Ctr, SB, Krasnoyarsk 660036, Russia.
Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119991, Russia.
Irkutsk State Univ, Biol Dept, Irkutsk 664003, Russia.
Siberian Fed Univ, Dept Biophys, Svobodny 79, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Rozhko, Tatiana V.; Kolesnik, Olga V.; Badun, Gennadii A.; Stom, Devard I.; Kudryasheva, Nadezhda S.; Kudryasheva, Nadezhda; RFBR-Krasnoyarsk Regional Foundation [N 18-44-242002, 18-44-240004]

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13.

Enzymatic responses to low-intensity radiation of tritium / T. V. Rozhko, E. V. Nemtseva, M. V. Gardt [et al.] // Int. J. Mol. Sci. - 2020. - Vol. 21, Is. 22. - Ст. 8464. - P1-15, DOI 10.3390/ijms21228464 . - ISSN 1661-6596
Кл.слова (ненормированные):
Bacterial luciferase -- Enzymes -- Fluorescent protein -- Hormesis -- Low-dose radiation -- Oxidoreductase -- Tritium
Аннотация: The present study considers a possible role of enzymatic reactions in the adaptive response of cells to the beta-emitting radionuclide tritium under conditions of low-dose exposures. Effects of tritiated water (HTO) on the reactions of bacterial luciferase and NAD(P)H:FMN-oxidoreductase, as well as a coupled system of these two reactions, were studied at radioactivity concentrations ? 200 MBq/L. Additionally, one of the simplest enzymatic reactions, photobiochemical proton transfer in Coelenteramide-containing Fluorescent Protein (CLM-FP), was also investigated. We found that HTO increased the activity of NAD(P)H:FMN-oxidoreductase at the initial stage of its reaction (by up to 230%); however, a rise of luciferase activity was moderate (<20%). The CLM-FP samples did not show any increase in the rate of the photobiochemical proton transfer under the exposure to HTO. The responses of the enzyme systems were compared to the ‘hormetic’ response of luminous marine bacterial cells studied earlier. We conclude that (1) the oxidoreductase reaction contributes significantly to the activation of the coupled enzyme system and bacterial cells by tritium, and (2) an increase in the organization level of biological systems promotes the hormesis phenomenon. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Scopus
Держатели документа:
Department of Medical and Biological Physics, Krasnoyarsk State Medical Academy, Krasnoyarsk, 660022, Russian Federation
Biophysics Department, Siberian Federal University, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics SB RAS, FRC KSC SB RAS, Krasnoyarsk, 660036, Russian Federation
Department of Chemistry, Moscow State University, Moscow, 119991, Russian Federation

Доп.точки доступа:
Rozhko, T. V.; Nemtseva, E. V.; Gardt, M. V.; Raikov, A. V.; Lisitsa, A. E.; Badun, G. A.; Kudryasheva, N. S.

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14.

Enzymatic Responses to Low-Intensity Radiation of Tritium / T. V. Rozhko, E. V. Nemtseva, M. V. Gardt [et al.] // Int. J. Mol. Sci. - 2020. - Vol. 21, Is. 22. - Ст. 8464, DOI 10.3390/ijms21228464. - Cited References:59. - This work was supported by RFBR-Krasnoyarsk Regional Foundation N 18-44-240004, 18-44-242002. . - ISSN 1422-0067

РУБ Biochemistry & Molecular Biology + Chemistry, Multidisciplinary
Рубрики:
LUMINOUS MARINE-BACTERIA
IONIZING-RADIATION
DISCHARGED-OBELIN
Кл.слова (ненормированные):
hormesis -- low-dose radiation -- tritium -- enzymes -- bacterial luciferase -- oxidoreductase -- fluorescent protein
Аннотация: The present study considers a possible role of enzymatic reactions in the adaptive response of cells to the beta-emitting radionuclide tritium under conditions of low-dose exposures. Effects of tritiated water (HTO) on the reactions of bacterial luciferase and NAD(P)H:FMN-oxidoreductase, as well as a coupled system of these two reactions, were studied at radioactivity concentrations <= 200 MBq/L. Additionally, one of the simplest enzymatic reactions, photobiochemical proton transfer in Coelenteramide-containing Fluorescent Protein (CLM-FP), was also investigated. We found that HTO increased the activity of NAD(P)H:FMN-oxidoreductase at the initial stage of its reaction (by up to 230%); however, a rise of luciferase activity was moderate (<20%). The CLM-FP samples did not show any increase in the rate of the photobiochemical proton transfer under the exposure to HTO. The responses of the enzyme systems were compared to the 'hormetic' response of luminous marine bacterial cells studied earlier. We conclude that (1) the oxidoreductase reaction contributes significantly to the activation of the coupled enzyme system and bacterial cells by tritium, and (2) an increase in the organization level of biological systems promotes the hormesis phenomenon.

WOS
Держатели документа:
Krasnoyarsk State Med Acad, Dept Med & Biol Phys, Krasnoyarsk 660022, Russia.
Siberian Fed Univ, Biophys Dept, Krasnoyarsk 660041, Russia.
RAS, Inst Biophys, SB, FRC,KSC, Krasnoyarsk 660036, Russia.
Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119991, Russia.

Доп.точки доступа:
Rozhko, Tatiana V.; Nemtseva, Elena V.; Gardt, Maria V.; Raikov, Alexander V.; Lisitsa, Albert E.; Badun, Gennadii A.; Kudryasheva, Nadezhda S.; Nemtseva, Elena; Kudryasheva, Nadezhda; Rozko, Tat'ana; Lisitsa, Albert; RFBR-Krasnoyarsk Regional Foundation [N 18-44-240004, 18-44-242002]

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15.

Adaptation of a Bacterial Bioluminescent Assay to Monitor Bioeffects of Gold Nanoparticles / M. R. Yehia, T. E. Smolyarova, A. V. Shabanov [et al.] // Bioengineering-Basel. - 2022. - Vol. 9, Is. 2. - Ст. 61, DOI 10.3390/bioengineering9020061. - Cited References:91 . - ISSN 2306-5354

РУБ Biotechnology & Applied Microbiology + Engineering, Biomedical

Кл.слова (ненормированные):
gold nanoparticles -- luminous marine bacteria -- bioassay -- hormesis -- tritium -- reactive oxygen species
Аннотация: Our current study aimed to adapt a bioluminescent bacteria-based bioassay to monitor the bioeffects of gold nanoparticles (AuNPs). Luminous marine bacteria Photobacterium phosphoreum and AuNPs modified with polyvinylpyrrolidone were employed; low-concentration (<= 10(-3) g/L) bioeffects of AuNPs were studied. Bioluminescence intensity was used as an indicator of physiological activity in bacteria. Two additional methods were used: reactive oxygen species (ROS) content was estimated with a chemiluminescent luminol method, and bacterial size was monitored using electron microscopy. The bacterial bioluminescent response to AuNPs corresponded to the "hormesis" model and involved time-dependent bioluminescence activation, as well as a pronounced increase in the number of enlarged bacteria. We found negative correlations between the time courses of bioluminescence and the ROS content in bacterial suspensions, demonstrating the relationship between bioluminescence activation and bacterial ROS consumption. The combined effects of AuNPs and a beta-emitting radionuclide, tritium, revealed suppression of bacterial bioluminescent activity (as compared to their individual effects) and a reduced percentage of enlarged bacteria. Therefore, we demonstrated that our bacteria-based bioluminescence assay is an appropriate tool to study the bioeffects of AuNPs; the bioeffects can be further classified within a unified framework for rapid bioassessment.

WOS
Держатели документа:
Siberian Fed Univ, Biophys Dept, Krasnoyarsk 660041, Russia.
RAS, Inst Phys SB, Fed Res Ctr, Krasnoyarsk Sci Ctr SB RAS, Krasnoyarsk 660036, Russia.
RAS, Inst Biophys SB, Fed Res Ctr, Krasnoyarsk Sci Ctr SB RAS, Krasnoyarsk 660036, Russia.
Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119991, Russia.

Доп.точки доступа:
Yehia, Moustafa R.; Smolyarova, Tatyana E.; Shabanov, Alexandr V.; Sushko, Ekaterina S.; Badun, Gennady A.; Kudryasheva, Nadezhda S.; Badun, Gennadii

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