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Closed artificial ecosystems as a means of ecosystem studies for earth and space needs / N. S. Pechurkin, I. M. Shirobokova // Advances in Space Research. - 2001. - Vol. 27, Is. 9. - P1497-1504, DOI 10.1016/S0273-1177(01)00244-7 . - ISSN 0273-1177
Кл.слова (ненормированные):
artificial ecosystem -- bioremediation -- biosphere -- ecosystem -- environmental monitoring -- model -- Bioremediation -- Ecology -- Ecosystems -- Health -- Biosphere -- Space research -- artificial ecosystem -- Biodegradation, Environmental -- Earth (Planet) -- Ecological Systems, Closed -- Ecology -- Ecosystem -- Energy Transfer -- Environmental Microbiology -- Life Support Systems -- Population Dynamics -- Yeasts
Аннотация: Closed Artificial ecosystems (CAES) have good prospects for wide use as new means for quantitative studies of different types of both natural ecosystems and man-made ones. The paper deals with the discussion of three points of CAES applications. The first one is of importance for theoretical ecology development and is connected with bringing together В«holisticВ» and В«merologicalВ» approaches in ecosystems studies. Using CAES, we can combine both approaches, taking into account the biotic turnover of limiting substrates which few in number even for complicated natural ecosystems. The second CAES use concerns the development of В«ecosystems healthВ» concept and application of a key-factor-approach for the indication and measurement of healthy unhealthy state and functioning of ecosystems or their links. The third use is more of an applied nature, oriented to the intensification of bioremediation or biodepollution processes in different types of ecosystems, including the global biosphere. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, 660036, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pechurkin, N.S.; Shirobokova, I.M.

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Modelling of genetically engineered microorganisms introduction in closed artificial microcosms / N. S. Pechurkin [et al.] // Advances in Space Research. - 1999. - Vol. 24, Is. 3. - P335-341, DOI 10.1016/S0273-1177(99)00320-8 . - ISSN 0273-1177
Кл.слова (ненормированные):
aquatic environment -- artificial ecosystem -- ecological modeling -- genetically modified organism -- alga -- animal -- article -- bacterial count -- bacterial gene -- biological model -- biomass -- Escherichia coli -- feasibility study -- genetic engineering -- genetics -- growth, development and aging -- microbiology -- microclimate -- Photobacterium -- plasmid -- protozoon -- time -- yeast -- Algae -- Animals -- Biomass -- Colony Count, Microbial -- Ecological Systems, Closed -- Escherichia coli -- Feasibility Studies -- Genes, Bacterial -- Genetic Engineering -- Models, Biological -- Photobacterium -- Plasmids -- Protozoa -- Time Factors -- Water Microbiology -- Yeasts
Аннотация: The possibility of introducing genetically engineered microorganisms (GEM) into simple biotic cycles of laboratory water microcosms was investigated. The survival of the recombinant strain Escherichia coli Z905 (Ap(r), Lux+) in microcosms depends on the type of model ecosystems. During the absence of algae blooming in the model ecosystem, the part of plasmid-containing cells E.coli decreased fast, and the structure of the plasmid was also modified. In conditions of algae blooming (Ankistrodesmus sp.) an almost total maintenance of plasmid-containing cells was observed in E.coli population. A mathematics model of GEM's behavior in water ecosystems with different level of complexity has been formulated. Mechanisms causing the difference in luminescent exhibition of different species are discussed, and attempts are made to forecast the GEM's behavior in water ecosystems.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russian Federation
Krasnoyarsk State University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pechurkin, N.S.; Brilkov, A.V.; Ganusov, V.V.; Kargatova, T.V.; Maksimova, E.E.; Popova, L.Yu.

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Interaction of a mixed yeast culture in an "autotroph-heterotroph" system with a closed atmosphere cycle and spatially separated components / T. I. Pisman, L. A. Somova // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1751-1756, DOI 10.1016/S0273-1177(03)00116-9 . - ISSN 0273-1177
Кл.слова (ненормированные):
Metabolism -- Oxygen -- Substrates -- Yeast -- Closed atmosphere cycle -- Space research -- carbon dioxide -- oxygen -- biosphere -- anaerobic growth -- article -- biomass -- Candida -- Chlorella -- comparative study -- culture medium -- ecosystem -- growth, development and aging -- metabolism -- microclimate -- Anaerobiosis -- Biomass -- Candida -- Carbon Dioxide -- Chlorella -- Culture Media -- Ecological Systems, Closed -- Ecosystem -- Environment, Controlled -- Oxygen
Аннотация: The study considers an experimental model of the "autotroph-heterotroph" system with a closed atmosphere cycle, in which the heterotrophic link is a mixed yeast population. The autotrophic link is represented by the algae Chlorella vulgaris and the heterotrophic link by the yeasts Candida utilis and Candida guilliermondii. The controls are populations of Chlorella and the same yeasts isolated from the atmosphere. It has been shown that the outcome of competition in the heterotrophic link depends on the strategy of the yeast population towards the substrate and oxygen. The C. utilis population quickly utilizes the substrate as it is an r-strategist and is less sensitive to oxygen deficiency. The C. guilliermondii population consumes low concentrations of the substrate because it is a K-strategist, but it is more sensitive to oxygen deficiency. That is why, in the "autotroph-heterotroph" system with a closed gas cycle, after a considerable amount of the substrate has been consumed, the C. guilliermondii population becomes more competitive that the C. utilis population. In the culture of yeasts, isolated from the atmosphere, the C. utilis population finds itself in more favorable conditions due to oxygen deficiency. The system with a complex heterotrophic component survive longer than a system whose heterotrophic component is represented by only one yeast species. This is explained for by the positive metabolite interaction of yeasts and a more complete utilization of the substrate by a mixed culture of yeasts featuring different strategies towards the substrate. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Inst. of Biophysics Siberian Branch, Russian Academy Science, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pisman, T.I.; Somova, L.A.

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EXPERIMENTAL-MODEL OF A GAS-CLOSED AUTOTROPH HETEROTROPH ECOSYSTEM [Text] / T. I. PISMAN [et al.] // Microbiology. - 1995. - Vol. 64, Is. 5. - P560-562. - Cited References: 7 . - ISSN 0026-2617

РУБ Microbiology

Аннотация: The dynamics of components were studied in a gas-closed autotroph-heterotroph ecosystem with spatially separated components. Oxygen-producing green microscopic algae served as the autotrophic component, and carbon dioxide-producing yeasts served as the heterotrophic component. The longevity of the gas-closed autotroph-heterotroph system was two times greater than that of separately cultivated components. Making the system more closed by additing glucose (the limiting factor for heterotroph growth) prolonged the longevity of the system up to 25 days. Further system closing by complicating the heterotrophic component (comprised now of two yeast species differing in the rate of substrate consumption) further increased the longevity of the system and the biomass of both the autotrophic and heterotrophic components.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
PISMAN, T.I.; SOMOVA, L.A.; SARANGOVA, A.B.; PECHURKIN, N.S.

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Interaction of a mixed yeast culture in an "autotroph-heterotroph" system with a closed atmosphere cycle and spatially separated components [Text] / T. I. Pisman, L. A. Somova ; ed. M Nelson [et al.] // SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P. 1751-1756, DOI 10.1016/S0273-1177(03)00116-9. - Cited References: 10 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Ecology + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences

Аннотация: The study considers an experimental model of the "autotroph-heterotroph" system with a closed atmosphere cycle, in which the heterotrophic link is a mixed yeast population. The autotrophic link is represented by the algae Chlorella vulgaris and the heterotrophic link by the yeasts Candida utilis and Candida guilliermondii. The controls are populations of Chlorella and the same yeasts isolated from the atmosphere. It has been shown that the outcome of competition in the heterotrophic link depends on the strategy of the yeast population towards the substrate and oxygen. The C. utilis population quickly utilizes the substrate as it is an r-strategist and is less sensitive to oxygen deficiency. The C guilliermondii population consumes low concentrations of the substrate because it is a K-strategist, but it is more sensitive to oxygen deficiency. That is why, in the "autotroph-heterotroph" system with a closed gas cycle, after a considerable amount of the substrate has been consumed, the C. guilliermondii population becomes more competitive that the C utilis population. In the culture of yeasts, isolated from the atmosphere, the C utilis population finds itself in more favorable conditions due to oxygen deficiency. The system with a complex heterotrophic component survive longer than a system whose heterotrophic component is represented by only one yeast species. This is explained for by the positive metabolite interaction of yeasts and a more complete utilization of the substrate by a mixed culture of yeasts featuring different strategies towards the substrate. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pisman, T.I.; Somova, L.A.; Nelson, M \ed.\; Pechurkin, NS \ed.\; Dempster, WF \ed.\; Somova, LA \ed.\; Somo, , LA \ed.\

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