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Carotenoids in bottom sediments of lake Shira as a paleoindicator for reconstruction of Lake States in Khakassiya, Russia / V. V. Zykov [et al.] // Contemporary Problems of Ecology. - 2012. - Vol. 5, Is. 4. - P434-442, DOI 10.1134/S199542551204018X . - ISSN 1995-4255
Кл.слова (ненормированные):
anaerobiosis -- bottom sediments -- holomixis -- meromixis -- okenone -- bacterium -- bioindicator -- biomarker -- carotenoid -- Holocene -- lacustrine deposit -- meromixis -- molecular analysis -- paleoclimate -- photoautotrophy -- reconstruction -- Khakassia -- Lake Shira -- Russian Federation
Аннотация: The concentrations of carotenoids buried in the bottom sediments of Lake Shira (Siberia, Khakassiya) have analyzed for the period of the last 2300 years. The bottom sediments were found to contain carotenoids, which are molecular markers of the corresponding groups of Phototrophic organisms. The bottom sediments of Lake Shira were shown to be a promising object for climate reconstructions of the Late Holocene in southern Siberia. В© 2012 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 5, build. 50, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, Svobodnyi pr. 79, Krasnoyarsk 660041, Russian Federation
Sobolev Institute of Geology and Mineralogy, Siberian Branch, Russian Academy of Sciences, Acedemika Koptyuga pr. 3, Novosibirsk 630090, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zykov, V.V.; Rogozin, D.Y.; Kalugin, I.A.; Dar'in, A.V.; Degermendzhi, A.G.

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Developing the control criterion for a continuous culture of microorganisms / V. V. Adamovich, D. Yu. Rogozin, A. G. Degermendzhi // Mikrobiologiya. - 2005. - Vol. 74, Is. 1. - С. 5-16 . - ISSN 0026-3656
Кл.слова (ненормированные):
Chemostat -- Control criterion -- Control factor -- Microorganism population -- Sensitivity coefficients -- algorithm -- bacterium -- biological model -- biomass -- culture medium -- ecosystem -- growth, development and aging -- methodology -- microbiological examination -- review -- Algorithms -- Bacteria -- Bacteriological Techniques -- Biomass -- Culture Media -- Ecosystem -- Models, Biological
Аннотация: A short survey and critical analysis of previously proposed criteria for growth control of populations of microorganisms in the chemostat are presented. Based on the analysis of a mathematical model of the steady-state of a microbial population in the chemostat, an adequate control criterion is suggested, along with a method to identify the corresponding regulating factors. The new control criterion is expressed as a product of the factor transformation coefficient and the biomass sensitivity coefficient (SC) with respect to the change of the factor at the chemostat inlet (referred to in the sequel as the biomass SC). The control criterion determines the strength of the control exerted by this or that factor. The method of determination of the regulating factors consists in experimental determination of the real SCs for factors and the biomass and in calculating on this basis the corresponding ideal SCs for constant factor transformation coefficients. The ideal SCs are shown to add up to an integer value, a constraint that we call "quantization" relationships. Such relationships are used to test the completeness of the drawn list of control factors. The proposed method was applied to our own and literature data.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Adamovich, V.V.; Rogozin, D.Yu.; Degermendzhi, A.G.

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Microbiological and isotopic-geochemical investigations of meromictic lakes in Khakasia in winter / A. S. Savvichev [и др.] // Mikrobiologiya. - 2005. - Vol. 74, Is. 4. - С. 552-561 . - ISSN 0026-3656
Кл.слова (ненормированные):
Meromictic water bodies -- Microbial production and oxidation of methane -- Photosynthesis -- Stable isotopes of carbon (? 13C) and sulfur (? 34S) -- Sulfate reduction -- Bacteria (microorganisms) -- Chlorobi -- Chromatiaceae -- Lamprocystis purpurea -- Pelodictyon luteolum -- Photobacteria -- carbon -- fresh water -- methane -- sulfate -- sulfur -- article -- bacterial phenomena and functions -- bacterium -- comparative study -- isolation and purification -- metabolism -- microbiology -- oxidation reduction reaction -- photosynthesis -- Russian Federation -- season -- species difference -- Bacteria -- Bacterial Physiology -- Carbon Isotopes -- Fresh Water -- Methane -- Oxidation-Reduction -- Photosynthesis -- Seasons -- Siberia -- Species Specificity -- Sulfates -- Sulfur Isotopes -- Water Microbiology
Аннотация: Microbiological and isotopic-geochemical investigations of the brackish meromictic lakes Shira and Shunet were performed in the steppe region of Khakasia in winter. Measurements made with a submersed sensor demonstrated that one-meter ice transmits light in a quantity sufficient for oxygenic and anoxygenic photosynthesis. As in the summer season, in the community of phototrophic bacteria found in Lake Shira, the purple sulfur bacteria Amoebobacter purpureus dominated, whereas, in Lake Shunet, the green sulfur bacteria Pelodictyon luteolum were predominant. Photosynthetic production, measured using the radioisotopic method, was several times lower than that in summer. The rates of sulfate reduction and production and oxidation of methane in the water column and bottom sediments were also lower than those recorded in summer. The process of anaerobic methane oxidation in the sediments was an exception, being more intense in winter than in summer. The data from radioisotopic measurements of the rates of microbial processes correlate well with the results of determination of the isotopic composition of organic and mineral carbon (? 13C) and hydrogen sulfide and sulfate (? 34S) and suggest considerable seasonal variations in the activity of the microbial community in the water bodies investigated.

Scopus
Держатели документа:
Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya, Moscow, 117811, Russian Federation
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk 36, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Savvichev, A.S.; Rusanov, I.I.; Rogozin, D.Yu.; Zakharova, E.E.; Lunina, O.N.; Bryantseva, I.A.; Yusupov, S.K.; Pimenov, N.V.; Degermendzhi, A.G.; Ivanov, M.V.

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Vertical stratification of physical, chemical and biological components in two saline lakes Shira and Shunet (South Siberia, Russia) / A. G. Degermendzhy [et al.] // Aquatic Ecology. - 2010. - Vol. 44, Is. 3. - P619-632, DOI 10.1007/s10452-010-9336-6 . - ISSN 1386-2588
Кл.слова (ненормированные):
Ciliates -- Cryptomonas -- Fishless lakes -- Gammarus -- Mathematical modelling -- Meromictic lakes -- Phytoplankton -- Stratification -- Sulphur bacteria -- amphipod -- bacterium -- biomass -- ciliate -- ecosystem modeling -- flagellate -- meromictic lake -- microbial community -- numerical model -- physicochemical property -- phytoplankton -- population density -- saline lake -- salinity -- stratification -- thermocline -- trophic interaction -- vertical profile -- zooplankton -- Khakassia -- Lake Shira -- Lake Shunet -- Russian Federation -- Siberia -- Amphipoda -- Bacteria (microorganisms) -- Ciliophora -- Copepoda -- Cryptomonas -- Cryptomonas sp. -- Gammaridae -- Gammarus -- Gammarus lacustris -- Phytomastigophorea -- Protista -- Rotifera
Аннотация: A feature of meromictic lakes is that several physicochemical and biological gradients affect the vertical distribution of different organisms. The vertical stratification of physical, chemical and biological components in saline, fishless meromictic lakes Shira and Shunet (Siberia, Russia) is quite different mainly because both mean depth and maximum depth of lakes differ as well as their salinity levels differ. The chemocline of the Lake Shira, as in many meromictic lakes, is inhabited by bacterial community consisting of purple sulphur and heterotrophic bacteria. As the depth of the chemocline is variable, the bacterial community does not attain high densities. The mixolimnion in Lake Shira, which is thermally stratified in summer, also creates different habitat for various species. The distribution of phytoplankton is non-uniform with its biomass peak in the metalimnion. The distribution of zooplankton is also heterogeneous with rotifers and juvenile copepods inhabiting the warmer epilimnion and older copepods found in the cold but oxic hypolimnion. The amphipod Gammarus lacustris which can be assigned to the higher trophic link in the fishless lake's ecosystem, such as Lake Shira, is also distributed non-uniformly, with its peak density generally observed in the thermocline region. The chemocline in Lake Shunet is located at the depth of 5 m, and unlike in Lake Shira, due to a sharp salinity gradient between the mixolimnion and monimolimnion, this depth is very stable. The mixolimnion in Lake Shunet is relatively shallow and the chemocline is inhabited by (1) an extremely dense bacterial community; (2) a population of Cryptomonas sp.; and (3) ciliate community comprising several species. As the mixolimnion of Lake Shunet is not thermally stratified for long period, the phytoplankton and zooplankton populations are not vertically stratified. The gammarids, however, tend to concentrate in a narrow layer located 1-2 m above the chemocline. We believe that in addition to vertical inhomogeneities of both physicochemical parameters, biological and physical factors also play a role in maintaining these inhomogeneities. We conclude that the stratified distributions of the major food web components will have several implications for ecosystem structure and dynamics. Trophic interactions as well as mass and energy flows can be significantly impacted by such heterogeneous distributions. Species spatially separated even by relatively short distances, say a few centimetres will not directly compete. Importantly, we demonstrate that not only bacteria, phytoflagellates and ciliate tend to concentrate in thin layers but also larger-sized species such Gammarus (amphipods) can also under certain environmental conditions have stratified distribution with maxima in relatively thin layer. As the vertical structure of the lake ecosystem is rather complex in such stratified lakes as ours, the strategy of research, including sampling techniques, should consider potentially variable and non-homogeneous distributions. В© 2010 The Author(s).

Scopus
Держатели документа:
Institute of Biophysics SB RAS, 660036 Krasnoyarsk, Akademgorodok, Russian Federation
Siberian Federal University, Svobodnyi 79, 660041 Krasnoyarsk, Russian Federation
Netherlands Environmental Assessment Agency (PBL), P.O. Box 303, 3720 AH Bilthoven, Netherlands
Department of Aquatic Ecology, Netherlands Institute of Ecology (NIOO-KNAW), 3631 AC Nieuwersluis, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Degermendzhy, A.G.; Zadereev, E.S.; Rogozin, D.Y.; Prokopkin, I.G.; Barkhatov, Y.V.; Tolomeev, A.P.; Khromechek, E.B.; Janse, J.H.; Mooij, W.M.; Gulati, R.D.

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Effect of winter conditions on distributions of anoxic phototrophic bacteria in two meromictic lakes in Siberia, Russia / D. Y. Rogozin [et al.] // Aquatic Ecology. - 2009. - Vol. 43, Is. 3. - P661-672, DOI 10.1007/s10452-009-9270-7 . - ISSN 1386-2588
Кл.слова (ненормированные):
Chemocline -- Green sulphur bacteria -- Purple sulphur bacteria -- Snow -- Winter -- anoxic conditions -- bacterium -- biomass -- ice cover -- light intensity -- meromictic lake -- photoautotrophy -- remote sensing -- vertical distribution -- Eurasia -- Khakassia -- Lake Shira -- Lake Shunet -- Russian Federation -- Siberia -- Bacteria (microorganisms) -- Photobacteria
Аннотация: The year-to-year variations of vertical distribution and biomass of anoxic phototrophic bacteria were studied during ice periods 2003-2005 and 2007-2008 in meromictic lakes Shira and Shunet (Southern Siberia, Russian Federation). The bacterial layers in chemocline of both lakes were sampled with a thin-layer hydraulic multi-syringe sampler. In winter, biomass of purple sulphur bacteria varied considerably depending on the amount of light penetrating into the chemocline through the ice and snow cover. In relatively weakly stratified, brackish Shira Lake, the depth of chemocline varied between winters, so that light intensity for purple sulphur bacteria inhabiting this zone differed. In Shira Lake, increased transparency of mixolimnion in winter, high chemocline position and absence of snow resulted in light intensity and biomass of purple sulphur bacteria exceeding the summer values in the chemocline of the lake. We could monitor snow cover at the lake surface using remote sensing and therefore estimate dynamics and amount of light under ice and its availability for phototrophic organisms. In Shunet Lake, the light intensities in the chemocline and biomasses of purple sulphur bacteria were always lower in winter than in summer, but the biomasses of green sulphur bacteria were similar. В© Springer Science+Business Media B.V. 2009.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok 50-50, 660036 Krasnoyarsk, Russian Federation
Siberian Federal University, Svobodny 79, 660071 Krasnoyarsk, Russian Federation
Center for Limnology, The Netherlands Institute of Ecology, Nieuwersluis, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Rogozin, D.Y.; Zykov, V.V.; Chernetsky, M.Y.; Degermendzhy, A.G.; Gulati, R.D.

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The "quantization" of sensitivity coefficients is preserved in microbial populations heterogeneous with respect to growth rate and age / V. M. Nekrasov, A. V. Chernychev, A. G. Degermendzhy // Doklady Biological Sciences. - 2006. - Vol. 406, Is. 1-6. - P91-93, DOI 10.1134/S0012496606010261 . - ISSN 0012-4966
Кл.слова (ненормированные):
algorithm -- article -- bacterium -- biodiversity -- growth, development and aging -- population dynamics -- theoretical model -- time -- Algorithms -- Bacteria -- Biodiversity -- Models, Theoretical -- Population Dynamics -- Time Factors

Scopus
Держатели документа:
Institute of Chemical Kinetics and Combustion, Siberian Division, Russian Academy of Sciences, Institutskaya ul. 3, Novosibirsk, 630090, Russian Federation
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Nekrasov, V.M.; Chernychev, A.V.; Degermendzhy, A.G.

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Cell growth and accumulation of polyhydroxyalkanoates from CO2 and H2 of a hydrogen-oxidizing bacterium, Cupriavidus eutrophus B-10646 / T. G. Volova [et al.] // Bioresource Technology. - 2013. - Vol. 146. - P215-222, DOI 10.1016/j.biortech.2013.07.070 . - ISSN 0960-8524
Кл.слова (ненормированные):
Autotrophic synthesis -- Cupriavidus eutrophus -- Polyhydroxyalkanoates
Аннотация: Synthesis of polyhydroxyalkanoates (PHAs) by a new strain of Cupriavidus - Cupriavidus eutrophus B-10646 - was investigated under autotrophic growth conditions. Under chemostat, at the specific flow rate D=0.1h-1, on sole carbon substrate (CO2), with nitrogen, sulfur, phosphorus, potassium, and manganese used as growth limiting elements, the highest poly(3-hydroxybutyrate) [P(3HB)] yields were obtained under nitrogen deficiency. In batch autotrophic culture, in the fermenter with oxygen mass transfer coefficient 0.460h-1, P(3HB) yields reached 85% of dry cell weight (DCW) and DCW reached 50g/l. Concentrations of supplementary PHA precursor substrates (valerate, hexanoate, ?-butyrolactone) and culture conditions were varied to produce, for the first time under autotrophic growth conditions, PHA ter- and tetra-polymers with widely varying major fractions of 3-hydroxybutyrate, 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate monomer units. Investigation of the high-purity PHA specimens showed significant differences in their physicochemical and physicomechanical properties. В© 2013 Elsevier Ltd.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
L.V. Kirenckii Institute of Physics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Institute of Chemistry and Chemical Technology of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kiselev, E.G.; Shishatskaya, E.I.; Zhila, N.O.; Boyandin, A.N.; Syrvacheva, D.A.; Vinogradova, O.N.; Kalacheva, G.S.; Vasiliev, A.D.; Peterson, I.V.

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Effects of intracellular poly(3-hydroxybutyrate) reserves on physiological-biochemical properties and growth of Ralstonia eutropha / T. G. Volova [et al.] // Research in Microbiology. - 2013. - Vol. 164, Is. 2. - P164-171, DOI 10.1016/j.resmic.2012.10.008 . - ISSN 0923-2508
Кл.слова (ненормированные):
Granules -- PHA cycle -- Poly(3-hydroxybutyrate) -- Ralstonia eutropha -- poly(3 hydroxybutyric acid) -- article -- bacterial growth -- bacterial strain -- bacterium culture -- cell division -- cell granule -- cell size -- controlled study -- Cupriavidus necator -- electron microscopy -- nonhuman -- polymer production -- priority journal -- Cupriavidus necator -- Cytoplasmic Granules -- Hydroxybutyrates -- Microscopy, Electron, Transmission -- Polyesters -- Bacteria (microorganisms) -- Cupriavidus necator
Аннотация: Microbial polyhydroxyalkanoates (PHAs), because of their well studied complex physiology and commercial potential, are vehicles for carbon and potential storage reduction for many microbial species. Even with the wealth of studies about microbial PHAs in the scientific literature, polymer accumulation and degradation are still not comprehensively understood. Poly(3-hydroxybutyrate) (P3HB) granule formation and polymer mobility were studied here in the bacterium Ralstonia eutropha strain B5786 in autotrophic cultures. Electron microscopy studies revealed decreasing cell size concomitant with enlargement of size and number of intracellular granules, and inhibition of cell division during intracellular polymer production. Activities of key P3HB biosynthetic enzymes demonstrated correlations with each other during polymer accumulation, suggesting an intricately regulated P3HB cycle in autotrophically grown R. eutropha cells. В© 2012 .

Scopus
Держатели документа:
Laboratory of Chemoautotrophic Biosynthesis, Institute of Biophysics of the Siberian Branch of The Russian Academy of Sciences, Akademgorodok 50, Krasnoyarsk 660036, Russian Federation
Department of Biotechnology, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Avenue, Krasnoyarsk 660041, Russian Federation
Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge 02139, MA, United States
Health Sciences Technology Division, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge 02139, MA, United States
Engineering Systems Division, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge 02139, MA, United States : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Zhila, N.O.; Kalacheva, G.S.; Brigham, C.J.; Sinskey, A.J.

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Biodegradation of polyhydroxyalkanoates (PHAs) in the South China Sea and identification of PHA-degrading bacteria / T. G. Volova [et al.] // Microbiology. - 2011. - Vol. 80, Is. 2. - P252-260, DOI 10.1134/S0026261711020184 . - ISSN 0026-2617
Кл.слова (ненормированные):
biodegradation in marine environments -- microbial PHA degraders -- PHA -- polyhydroxyalkanoates -- Bacillus (bacterium) -- Bacillus sp. -- Bacteria (microorganisms) -- Enterobacter -- Enterobacter cloacae -- Gracilibacillus -- Prospect Hill virus
Аннотация: The biodegradation patterns of two types of PHA, a 3-hydroxybutyrate (3-PHB) polymer and a 3-hydroxybutyrate and 3-hydroxyvalerate (3-PHB/3-PHV) copolymer, were studied in tropical marine environments (Dam Bay, South China Sea, Nha Trang, Vietnam). No reliable differences in the degradation of 3-PHB and 3-PHB/3-PHV were revealed. It was shown that the degradation process depended mainly on the shape of a polymer product and its production method: the degradation of polymer films was found to be more active than that of molded solids. A decrease in the molecular mass of both types of PHA was detected in the course of the degradation of PHA samples. However, the degree of PHA crystallinity did not change; that is, the levels of degradation of both the amorphous and crystalline phases of PHA were almost the same. Among microbial PHA degraders, three bacterial strains, Bacillus sp. IBP-V002, Enterobacter cloacae sp. IBP-V001, and Gracilibacillus sp. IBP-V003, were identified based on the results of morphological, biochemical, and molecular phylogenetic analyses. The ability of the representatives of the genera Gracilibacillus and Enterobacter to degrade PHA was revealed for the first time. В© 2011 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Kirensky Institute of Physics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, Russian Federation
Marine Department, Joint Russian-Vietnamese Tropical Research and Test Center, Nha Trang, Viet Nam : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Boyandin, A.N.; Vasil'ev, A.D.; Karpov, V.A.; Kozhevnikov, I.V.; Prudnikova, S.V.; Rudnev, V.P.; Xuan, B.B.; Dung, V.V.; Gitel'zon, I.I.

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10.

Biosynthesis of multi-component polyhydroxyalkanoates by the bacterium Wautersia eutropha / T. G. Volova, G. S. Kalacheva, A. Steinbuchel // Macromolecular Symposia. - 2008. - Vol. 269, Is. 1. - P1-7, DOI 10.1002/masy.200850901 . - ISSN 1022-1360
Кл.слова (ненормированные):
Autotrophic and mixotrophic growth -- Multi-component polyhydroxyalkanoates -- Wautersia eutropha -- Bacteriology -- Biochemical engineering -- Biochemistry -- Biopolymers -- Biotechnology -- Carboxylic acids -- Fatty acids -- Ketones -- Monomers -- Polymers -- Autotrophic and mixotrophic growth -- Carbon chains -- Carbon nutritions -- Even numbers -- Growth conditions -- Hydroxybutyrate -- Hydroxyvalerate -- Mixotrophic -- Multi-component polyhydroxyalkanoates -- Odd numbers -- Physico-chemical properties -- Polyhydroxyalkanoates -- Synthesis of -- Wautersia eutropha -- Wild types -- Acids
Аннотация: The study addresses the effect of different conditions of carbon nutrition on synthesis of polyhydroxyalkanoates by the bacterium Wautersia eutropha. In experiments with two wild type strains (H16 and 65786), it has been first found that under mixotrophic growth conditions - Co2 + co-substrate (alkanoic acids) - bacteria can synthesize multi-component PHAs, consisting of short- and medium-chainlength monomers with carbon chains containing 4 to 8 atoms. It has been shown that PHA composition is determined by the type of the co-substrate. Fatty acids with odd number of carbons induce bacteria to synthesize four- and five-component PHAs with hydroxybutyrate, hydroxyvalerate as major monomers and hydroxyhexanoate, hydroxyheptanoate and hydroxyoctanoate as minor, occasionally occurring, ones. Fatty acids with even number of carbons induce synthesis of not only their respective monomers (hydroxyhexanoate and hydroxyoctanoate) but also hydroxyvalerate, making possible synthesis of four-component PHAs, containing hydroxybutyrate and hydroxyhexanoate as major components (up to 18 mol%). A family of short- and medium-chain-length four- and five-component PHAs were synthesized and their physicochemical properties examined. Copyright В© 2008 WILEY-VCH Verlag GmbH & Co. KGaA.

Scopus
Держатели документа:
Institute of Biophysics, SB, RAS, 660036 Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Molecular Microbiology and Biotechnology, Munster, Germany : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Steinbuchel, A.

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11.

Luminous bacteria as potential producers of resorbed polyhydroxyalkanoate polyesters / A. N. Boyandin [et al.] // Doklady Biochemistry and Biophysics. - 2007. - Vol. 416, Is. 1-6. - P248-251, DOI 10.1134/S1607672907050067 . - ISSN 1607-6729
Кл.слова (ненормированные):
photoprotein -- polyester -- polyhydroxyalkanoic acid -- article -- bacterium -- chemistry -- chemoluminescence -- classification -- genetic procedures -- genetics -- metabolism -- Photobacterium -- phylogeny -- time -- Bacteria -- Biosensing Techniques -- Chemiluminescent Measurements -- Luminescent Proteins -- Photobacterium -- Phylogeny -- Polyesters -- Polyhydroxyalkanoates -- Time Factors -- Bacteria (microorganisms)

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Kalacheva, G.S.; Rodicheva, E.K.; Volova, T.G.

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12.

Physicochemical properties of multicomponent poly(hydroxyalkanoates) / T. G. Volova, P. V. Mironov, A. D. Vasil'ev // Biophysics. - 2007. - Vol. 52, Is. 3. - P293-297, DOI 10.1134/S0006350907030062 . - ISSN 0006-3509
Кл.слова (ненормированные):
Hydroxyhexanoate -- Hydroxyvalerate -- Poly(hydroxybutyrate) -- Bacteria (microorganisms) -- Cupriavidus necator -- Insectivora
Аннотация: The properties of new five-component poly(hydroxyalkanoates) (PHA) formed by short-and medium-chain monomers synthesized by the bacterium Wautersia eutropha B5786 were studied by X-ray diffraction, IR spectroscopy, differential thermal analysis, and viscometry. The degree of crystallinity of PHA decreased from 72 to 57% as the molar fraction of hydroxyhexanoate increased from 2.5 to 18.0 mol%. The melting temperature (T m) and decomposition temperature (T d) of the multicomponent PHA are lower than those for poly(hydroxybutyrate), whose T m and T d are 168-170 and 260-265В°C, respectively. Both parameters of the multicomponent PHA decrease to 156 and 252В°C, respectively, as the hydroxyhexanoate mole fraction is raised. The effect of hydroxyhexanoate on the physicochemical properties of the PHA is similar to that of hydroxyvalerate observed previously. В© 2007 Pleiades Publishing, Inc.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation
Siberian State Technological University, Krasnoyarsk 660049, Russian Federation
Kirenskii Institute of Physics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Mironov, P.V.; Vasil'ev, A.D.

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13.

Physiological-biochemical properties and the ability to synthesize polyhydroxyalkanoates of the glucose-utilizing strain of the hydrogen bacterium Ralstonia eutropha B8562 / T. G. Volova [et al.] // Applied Microbiology and Biotechnology. - 2006. - Vol. 73, Is. 3. - P429-433, DOI 10.1007/s00253-006-0460-0 . - ISSN 0175-7598
Кл.слова (ненормированные):
Biochemistry -- Carbon -- Genetic engineering -- Glucose -- Hydrogen -- Polyesters -- Cultural properties -- Glucose-utilizing mutant strain -- Nitrogen deficiency -- Polyhydroxyalkanoate (PHA) -- Bacteria -- 3 hydroxybutyric acid -- carbon dioxide -- fructose -- glucose -- hydrogen -- monomer -- polyhydroxyalkanoic acid -- sugar -- bacterium -- carbon -- comparative study -- energy -- ester -- experimental study -- glucose -- hydrogen -- physicochemical property -- article -- bacterial strain -- bacterium culture -- bacterium mutant -- biomass -- chemical analysis -- Cupriavidus necator -- energy consumption -- genetic analysis -- nonhuman -- synthesis -- Bacteria (microorganisms) -- Cupriavidus necator
Аннотация: Physiological-biochemical, genetic, and cultural properties of the glucose-utilizing mutant strain Ralstonia eutropha B8562 have been compared with those of its parent strain R. eutropha B5786. It has been shown that growth characteristics of the strain cultured on glucose as the sole carbon and energy source are comparable with those of the parent strain. Strain B8562 is characterized by high polyhydroxyalkanoate (PHA) yields on different carbon sources (CO2, fructose, and glucose). PHA accumulation in the strain batch cultured on glucose under nitrogen deficiency reaches 90 %. The major monomer in the PHA is ?-hydroxybutyric acid (more than 99 mol %); the identified minor components are ?-hydroxyvaleric acid (0.25-0.72 mol %) and ?-hydroxyhexanoic acid (0.08-1.5 mol %). The strain is a promising PHA producer on available sugar-containing media with glucose. В© Springer-Verlag 2006.

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Держатели документа:
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russian Federation
Krasnoyarsk State University, Svobodny av. 79, 660041 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Trusova, M.Y.; Kalacheva, G.S.; Kozhevnicov, I.V.

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14.

Physiological-biochemical properties and the ability to synthesize polyhydroxyalkanoates of the glucose-utilizing strain of the hydrogen bacterium Ralstonia eutropha B8562. / T. G. Volova [et al.] // Applied microbiology and biotechnology. - 2006. - Vol. 73, Is. 2. - P429-433, DOI 10.1007/s00253-006-0460-0 . - ISSN 0175-7598
Кл.слова (ненормированные):
beta hydroxyvaleric acid -- beta-hydroxyvaleric acid -- carbon dioxide -- ester -- fatty acid -- fructose -- glucose -- hexanoic acid derivative -- hydrogen -- hydroxyacid -- oxygen -- polymer -- ribosome RNA -- valeric acid derivative -- article -- biotechnology -- chemistry -- metabolism -- methodology -- Wautersia eutropha -- Biotechnology -- Carbon Dioxide -- Cupriavidus necator -- Esters -- Fatty Acids -- Fructose -- Glucose -- Hexanoic Acids -- Hydrogen -- Hydroxy Acids -- Oxygen -- Pentanoic Acids -- Polymers -- RNA, Ribosomal
Аннотация: Physiological-biochemical, genetic, and cultural properties of the glucose-utilizing mutant strain Ralstonia eutropha B8562 have been compared with those of its parent strain R. eutropha B5786. It has been shown that growth characteristics of the strain cultured on glucose as the sole carbon and energy source are comparable with those of the parent strain. Strain B8562 is characterized by high polyhydroxyalkanoate (PHA) yields on different carbon sources (CO(2), fructose, and glucose). PHA accumulation in the strain batch cultured on glucose under nitrogen deficiency reaches 90 %. The major monomer in the PHA is beta-hydroxybutyric acid (more than 99 mol %); the identified minor components are beta-hydroxyvaleric acid (0.25-0.72 mol %) and beta-hydroxyhexanoic acid (0.08-1.5 mol %). The strain is a promising PHA producer on available sugar-containing media with glucose.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Trusova, M.Y.; Kalacheva, G.S.; Kozhevnicov, I.V.

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15.

The synthesis of hydroxybutyrate and hydroxyvalerate copolymers by the bacterium Ralstonia eutropha / T. G. Volova, G. S. Kalacheva // Mikrobiologiya. - 2005. - Vol. 74, Is. 1. - С. 63-69 . - ISSN 0026-3656
Кл.слова (ненормированные):
?-ketothiolase -- Controlled synthesis -- Poly(hydroxybutyrate-co-hydroxyvalerate) -- Ralstonia eutropha -- Bacteria (microorganisms) -- Cupriavidus necator -- acetoacetyl coenzyme a -- acetyl coenzyme A acyltransferase -- acyl coenzyme A -- acyltransferase -- butyric acid derivative -- carbon dioxide -- fructose -- hydrogen -- poly(3 hydroxybutyrate) co (3 hydroxyvalerate) -- poly(3-hydroxyalkanoic acid) synthase -- poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate) -- polyester -- polyhydroxyalkanoate synthase -- valeric acid derivative -- article -- chemistry -- crystallization -- culture medium -- metabolism -- Wautersia eutropha -- Acetyl-CoA C-Acyltransferase -- Acyl Coenzyme A -- Acyltransferases -- Butyrates -- Carbon Dioxide -- Crystallization -- Culture Media -- Cupriavidus necator -- Fructose -- Hydrogen -- Polyesters -- Valerates
Аннотация: The paper deals with the study of the synthesis of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) copolymers by the bacterium Ralstonia eutropha B-5786 grown under different carbon nutrition conditions (growth on carbon dioxide, fructose, and CO2-valerate and fructose-valerate mixtures). The parameters to be analyzed included the yield of biomass, the yield, synthesis rate, and composition of copolymers, the activity of the key enzymes of polyhydroxyalkanoate (PHA) synthesis (?-ketothiolase, acetoacetyl-CoA reductase, and PHA synthase), the maximum tolerable concentration of valerate to the bacterium, and the conditions that govern the incorporation of hydroxyvalerate to copolymers. This allowed the relationship between cultivation conditions and the proportion of monomers in the copolymers to be deduced. We were able to synthesize a range of 3HB/3HV copolymers and found that the thermal characteristics and the degree of crystallinity of these copolymers depend on the molar fraction of 3HV.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.

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16.

The synthesis of hydroxybutyrate and hydroxyvalerate copolymers by the bacterium Ralstonia eutropha / T. G. Volova, G. S. Kalacheva // Microbiology. - 2005. - Vol. 74, Is. 1. - P54-59, DOI 10.1007/s11021-005-0028-5 . - ISSN 0026-2617
Кл.слова (ненормированные):
?-ketothiolase -- Controlled synthesis -- Poly(hydroxybutyrate-co-hydroxyvalerate) -- Ralstonia eutropha -- Bacteria (microorganisms) -- Ralstonia -- Wautersia eutropha
Аннотация: The paper deals with the study of the synthesis of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) copolymers by the bacterium Ralstonia eutropha B-5786 grown under different carbon nutrition conditions (growth on carbon dioxide, fructose, and CO2-valerate and fructose-valerate mixtures). The parameters to be analyzed included the yield of biomass; the yield, synthesis rate, and composition of copolymers; the activity of the key enzymes of polyhydroxyalkanoate (PHA) synthesis ?-ketothiolase, acetoacetyl-CoA reductase, and PHA synthase); the maximum tolerable concentration of valerate to the bacterium; and the conditions that govern the incorporation of hydroxyvalerate into copolymers. This allowed the relationship between cultivation conditions and the proportion of monomers in the copolymers to be deduced. We were able to synthesize a range of 3HB/3HV copolymers and found that the thermal characteristics and the degree of crystallinity of such copolymers depend on the molar fraction of 3HV. В© 2005 Pleiades Publishing, Inc.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.

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17.

Study of a Ralstonia eutropha culture producing polyhydroxyalkanoates on products of coal processing / T. G. Volova, N. A. Voinov // Applied Biochemistry and Microbiology. - 2004. - Vol. 40, Is. 3. - P249-252, DOI 10.1023/B:ABIM.0000025946.47013.03 . - ISSN 0003-6838
Кл.слова (ненормированные):
alkanoic acid -- carbon monoxide -- coal -- hydrogen -- lignite -- algorithm -- article -- bacterial strain -- bacterium culture -- coal gasification -- controlled study -- gas -- gas exchange -- mathematical computing -- nonhuman -- Ralstonia eutropha -- Bacteria (microorganisms) -- Ralstonia -- Wautersia eutropha
Аннотация: Kinetic indices of growth, polyhydroxyalkanoate (PHA) accumulation, and gas exchange were studied in a culture of the carbon monoxide-resistant hydrogen strain Ralstonia eutropha B-5786 grown on a gaseous substrate (GS) obtained by lignite gasification. The GS was shown to be suitable for PHA production. To increase the degree of GS consumption, various modes of gas supply to the culture were tested. Based on the results, an algorithm was developed for calculating and controlling gas-exchange parameters in the PHA-accumulating culture of Ralstonia eutropha, grown on a new GS allowing high polymer yields (up to 75%) and degrees of substrate utilization (up to 90%).

Scopus
Держатели документа:
Institute of Biophysics, Siberian Div. Russ. Acad. of Sci., Krasnoyarsk, 660036, Russian Federation
Siberian State Technol. University, Min. of Educ. of the Russ. Fed., Krasnoyarsk, 660049, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Voinov, N.A.

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18.

Dynamics of activity of the key enzymes of polyhydroxyalkanoate metabolism in Ralstonia eutropha B5786 / T. G. Volova [et al.] // Applied Biochemistry and Microbiology. - 2004. - Vol. 40, Is. 2. - P170-177, DOI 10.1023/B:ABIM.0000018921.04863.d5 . - ISSN 0003-6838
Кл.слова (ненормированные):
acetyl coenzyme A acyltransferase -- bacterial enzyme -- carbon -- carbon dioxide -- fructose -- hydrogen -- hydroxybutyrate dehydrogenase -- oxidoreductase -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- synthetase -- article -- bacterial metabolism -- carbon source -- catalysis -- controlled study -- degradation -- depolymerization -- enzyme activity -- enzyme analysis -- molecular dynamics -- nonhuman -- protein function -- Ralstonia eutropha -- recording -- statistical significance -- synthesis -- Bacteria (microorganisms) -- Ralstonia -- Wautersia eutropha
Аннотация: The dynamics of accumulation of polyhydroxybutyrate (PHB) and the activities of key enzymes of PHB metabolism (?-ketothiolase, acetoacetyl-CoA reductase, PHB synthase, D-hydroxybutyrate dehydrogenase, and PHB depolymerase) in the hydrogen bacterium Ralstonia eutropha B5786 were studied under various conditions of carbon nutrition and substrate availability. The highest activities of ?-ketothiolase, acetoacetyl-CoA reductase, and PHB synthase were recorded during acceleration of PHB synthesis. The activities of enzymes catalyzing PHB depolymerization (PHB depolymerase and D-hydroxybutyrate dehydrogenase) were low, being expressed only upon stimulated endogenous PHB degradation. The change of carbon source (CO2 or fructose) did not affect the time course of the enzyme activity significantly.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Gorbunova, O.V.; Zhila, N.O.

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19.

Dynamics of activity of the key enzymes of polyhydroxyalkanoate metabolism in Ralstonia eutropha / T. G. Volova [и др.] // Prikladnaia biokhimiia i mikrobiologiia. - 2004. - Vol. 40, Is. 2. - С. 201-209 . - ISSN 0555-1099
Кл.слова (ненормированные):
acetoacetyl coenzyme a reductase -- acetoacetyl-CoA reductase -- acetyl coenzyme A acyltransferase -- acyltransferase -- alcohol dehydrogenase -- carboxylesterase -- hydroxybutyrate dehydrogenase -- hydroxybutyric acid -- poly(3 hydroxyalkanoic acid) depolymerase -- poly(3-hydroxyalkanoic acid) depolymerase -- poly(3-hydroxyalkanoic acid) synthase -- polyhydroxyalkanoate synthase -- polymer -- article -- chemistry -- comparative study -- culture medium -- enzymology -- growth, development and aging -- metabolism -- Wautersia eutropha -- Acetyl-CoA C-Acyltransferase -- Acyltransferases -- Alcohol Oxidoreductases -- Carboxylic Ester Hydrolases -- Culture Media -- Cupriavidus necator -- Hydroxybutyrate Dehydrogenase -- Hydroxybutyrates -- Polymers
Аннотация: The dynamics of accumulation of polyhydroxybutyrate (PHB) and the activities of the key enzymes of PHB metabolism (beta-ketothiolase, acetoacetyl-CoA reductase, PHA synthase, D-hydroxybutyrate dehydrogenase, and PHA depolymerase) in the hydrogen bacterium Ralstonia eutropha B5786 were studied under various conditions of carbon nutrition and substrate availability. The highest activities of beta-ketothiolase, acetoacetyl-CoA reductase, and PHA synthase were recorded at the stage of acceleration of PHB synthesis. The activities of enzymes catalyzing PHB depolymerization (PHB depolymerase and D-hydroxybutyrate dehydrogenase) were low, being expressed only at stimulated endogenous PHB degradation. The change of carbon source (CO2 or fructose) did not cause any marked changes in the time course of enzyme activity.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Gorbunova, O.V.; Zhila, N.O.

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20.

Production of purified polyhydroxyalkanoates (PHAs) for applications in contact with blood / V. I. Sevastianov [et al.] // Journal of Biomaterials Science, Polymer Edition. - 2003. - Vol. 14, Is. 10. - P1029-1042, DOI 10.1163/156856203769231547 . - ISSN 0920-5063
Кл.слова (ненормированные):
?-hydroxy acids -- Endotoxins -- Hemocompatibility -- Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) -- Polyhydroxyalkanoates (PHAs) -- Polyhydroxybutyrate (PHB) -- bacterium lipopolysaccharide -- carbon -- complement -- copolymer -- hydroxyacid -- long chain fatty acid -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- valeric acid derivative -- adult -- article -- biofilm -- biotechnology -- blood analysis -- blood clotting -- blood compatibility -- cell function -- chemical analysis -- chemical composition -- complement activation -- concentration (parameters) -- controlled study -- gas chromatography -- hemostasis -- human -- human cell -- mass spectrometry -- micromorphology -- nonhuman -- priority journal -- purification -- quantitative analysis -- sampling -- synthesis -- thrombocyte adhesion -- Wautersia eutropha -- Biocompatible Materials -- Blood -- Blood Coagulation Tests -- Chromatography, Gas -- Complement Activation -- Cupriavidus necator -- Fatty Acids -- Humans -- Platelet Adhesiveness -- Polyesters -- Surface Properties
Аннотация: Samples of olyhydroxyalkanoates (PHAs), polyhydroxybutyrate (PHB) and copolymers poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) with 4 and 18 mol% hydroxyvalerate, synthesized by the bacteria Ralstonia eutropha B5786, were investigated. PHA films in contact with blood did not activate the hemostasis system at the level of cell response, but they did activate the coagulation system and the complement reaction. To detect biologically-active components in the PHAs, a detailed analysis of the composition of the polymers was conducted. Gas chromatography-mass spectrometry revealed long-chain fatty acids (FAs) in the tested PHAs. Their total concentration in the polymer ranged from tenths of mol% to 2-3 mol%, depending on the purification method. C16:0 constituted the largest proportion, up to 70%. Of the long-chain hydroxy acids, only ?-OH-C14:0 was detected and it did not exceed 0.06 mol%. The analysis of the hemocompatibility properties of the PHAs purified by a specialized procedure, including the quantitative and morphological estimation of platelets adherent to the surface of polymer films, the plasma recalcification time and complement activation studies, indicated that PHB and PHBV can be used in contact with blood. It has been found out that the lipopolysaccharides of bacteria producing PHAs, which contain mostly long-chain hydroxy acids, can be the factor activating the hemostasis systems. Thus, the technology of PHA purification must satisfy rather stringent specific requirements.

Scopus
Держатели документа:
Inst. of Transplantol. Artif. Organs, Russian Ministry of Health, Shchukinskaya 1, 123182 Moscow, Russian Federation
Inst. of Biophys. of Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sevastianov, V.I.; Perova, N.V.; Shishatskaya, E.I.; Kalacheva, G.S.; Volova, T.G.

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