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Growth and luminescence of luminous bacteria promoted by agents of microbial origin. / E. K. Rodicheva [et al.] // Journal of bioluminescence and chemiluminescence. - 1993. - Vol. 8, Is. 6. - P293-299 . - ISSN 0884-3996
Кл.слова (ненормированные):
amino acid -- carbohydrate -- folic acid -- luciferase -- nitrogen -- riboflavin -- article -- biosynthesis -- culture medium -- electron microscopy -- growth, development and aging -- kinetics -- luminescence -- metabolism -- Photobacterium -- physiology -- time -- ultrastructure -- Vibrio -- Amino Acids -- Carbohydrates -- Culture Media -- Folic Acid -- Kinetics -- Luciferase -- Luminescence -- Microscopy, Electron -- Nitrogen -- Photobacterium -- Riboflavin -- Time Factors -- Vibrio
Аннотация: The examination of four species of luminous bacteria Photobacterium leiognathi, Photobacterium phosphoreum, Vibrio fischeri and Vibrio harveyi has enabled us to reveal some nutrient medium components effecting growth, luminescence intensity and luciferase synthesis. These agents are nucleic components (nucleotides, nucleotides and amine bases), amino acids and vitamins, which are part of hydrolysates from the biomass of various lithotrophic microorganisms, hydrogen-oxidizing, iron-oxidizing and carboxydobacteria. The effect of promoting agents essentially alters the physiological state and ultrastructure of the cells of luminous bacteria and increases luciferase biosynthesis two- to three-fold compared to a control.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Krasnoyarsk. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Rodicheva, E.K.; Trubachev, I.N.; Medvedeva, S.E.; Egorova, O.I.; , U - Shitova LYu

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Model approaches to description of critical phenomena in forest ecosystems / A. S. Isaev, V. G. Soukhovolsky, R. G. Khlebopros // Contemporary Problems of Ecology. - 2011. - Vol. 4, Is. 7. - P699-705, DOI 10.1134/S1995425511070010 . - ISSN 1995-4255
Кл.слова (ненормированные):
critical phenomena -- ecosystem -- modeling -- phase transitions -- population -- population dynamics -- data set -- ecosystem modeling -- forest ecosystem -- forest fire -- insect -- optimization -- population dynamics -- succession -- Hexapoda
Аннотация: Methodological bases of optimization modeling approach are considered in regard to describing critical phenomena in forest ecosystems. Models of insects' outbreaks, forest successions, forest fires as second order phase transitions are proposed. It is shown that there is fair correlation between model calculations and observations data. В© 2011 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Center on the Problems of Ecology and Productivity of Forests, Russian Academy of Sciences, ul. Profsoyuznaya 84/32, Moscow 117997, Russian Federation
Sukachev Institute of Forest, Siberian Branch of the Russian Academy of Sciences, Akademgorodok 50/28, Krasnoyarsk 660036, Russian Federation
Intern Cntr 4 Rrch o Extr. St. of Organi w in Pres., of Krasnoyarsk Res Ctr of Sib Brnch of the Russ. Ac of Sc, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Isaev, A.S.; Soukhovolsky, V.G.; Khlebopros, R.G.

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Nonequilibrium capillary electrophoresis of equilibrium mixtures, mathematical model [Text] / V. . Okhonin, S. M. Krylova, S. N. Krylov // Anal. Chem. - 2004. - Vol. 76, Is. 5. - P. 1507-1512, DOI 10.1021/ac035259p. - Cited References: 24 . - ISSN 0003-2700

РУБ Chemistry, Analytical
Рубрики:
SURFACE-PLASMON RESONANCE
   PROTEIN-DNA INTERACTIONS
   FLUORESCENCE POLARIZATION
   COMPLEX-FORMATION
   BINDING
   RECOGNITION
   DERIVATIVES
   TOOL
Аннотация: We recently introduced a new electrophoretic method, nonequilibrium, capillary electrophoresis of equilibrium mixtures (NECEEM). NECEEM provides a unique way of finding kinetic and equilibrium parameters of the formation of intermolecular complexes from a single electropherogram and allows for the use of weak affinity probes in protein quantitation. In this work, we study theoretical bases of NECEEM by developing a mathematical model for the new method. By solving a system of partial differential equations with diffusion in linear approximation, we found the analytical solution for concentrations of components involved in complex formation as functions of time from the beginning of separation and position in the capillary. The nonnumerical nature of the solution makes it a powerful tool in studying the theoretical foundations of the NECEEM method and modeling experimental results. We demonstrate the use of the model for finding binding parameters of complex formation by nonlinear regression of NECEEM electropherograms obtained experimentally.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Okhonin, V...; Krylova, S.M.; Krylov, S.N.

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Distribution of heterotrophic bacteria in Lake Shira [Text] / T. I. Lobova, L. V. Listova, L. Y. Popova // Microbiology. - 2004. - Vol. 73, Is. 1. - P. 89-93, DOI 10.1023/B:MICI.0000016375.87884.fc. - Cited References: 12 . - ISSN 0026-2617

РУБ Microbiology

Кл.слова (ненормированные):
saline lakes -- halotolerance -- mesophilic and psychrotolerant heterotrophic bacteria -- autochthonous and allochthonous microorganisms
Аннотация: A study of the horizontal and vertical distribution of heterotrophic bacteria in brackish Lake Shira in summer periods showed that mesophilic bacteria dominated in all areas of the lake, whereas psychrotolerant bacteria dominated in the metalimnion and hypolimnion of its central part. Nonhalophilic bacteria were mostly mesophilic and dominated in coastal waters. Most psychrotolerant bacteria were able to grow in the presence of 5-10% NaCl. Heterotrophic bacteria isolated in different regions of the lake were identified to a generic level. The isolates were classified into autochthonous and allochthonous microorganisms on the bases of their distribution pattern in the lake water, halotolerance, and ability to grow at low temperatures.

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Держатели документа:
Russian Acad Sci, Siberian Div, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Lobova, T.I.; Listova, L.V.; Popova, L.Y.

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Nonspecific stress response to temperature increase in Gammarus lacustris Sars with respect to oxygen-limited thermal tolerance concept / K. Vereshchagina [et al.] // PeerJ. - 2018. - Vol. 6. - Ст. e5571, DOI 10.7717/peerj.5571. - Cited References:49. - The study was carried out with the main financial support of Russian Science Foundation grant 17-14-01063, with the partial financial support of Russian Foundation for Basic Research grants 16-34-00687, 16-34-60060, 17-34-50012, the base part of Goszadanie project 6.9654.2017/8.9, joint program of DAAD and Ministry of education and Science M. Lomonosov (6.12735.2018/12.2) and Lake Baikal Foundation (FOB_02-3/05). There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. . - ISSN 2167-8359

РУБ Multidisciplinary Sciences
Рубрики:
COPEPOD TIGRIOPUS-JAPONICUS
SHUNET SOUTH SIBERIA
HEAT-SHOCK PROTEINS
Кл.слова (ненормированные):
Gammarus lacustris -- Heat shock proteins 70 (HSP70) -- Nonspecific cellular -- stress-response (NCSR) -- Lactate dehydrogenase -- Diene conjugates -- Schiff -- bases -- Triene conjugates
Аннотация: The previously undescribed dynamics of the heat shock protein HSP70 and subsequent lipid peroxidation products have been assessed alongside lactate dehydrogenase activity for Gammarus lacustris Sars, an amphipod species from the saltwater Lake Shira (Republic of Khakassia). Individuals were exposed to a gradual temperature increase of 1 degrees C/hour (total exposure duration of 26 hours) starting from the mean annual temperature of their habitat (7 degrees C) up to 33 degrees C. A complex of biochemical reactions occurred when saltwater G. lactustris was exposed to the gradual changes in temperature. This was characterized by a decrease in lactate dehydrogenase activity and the launching of lipid peroxidation. The HSP70 level did not change significantly during the entire experiment. In agreement with the concept of oxygen-limited thermal tolerance, an accumulation of the most toxic lipid peroxides (triene conjugates and Schiff bases) in phospholipids occurred at the same time and temperature as the accumulation of lactate. The main criterion overriding the temperature threshold was, therefore, the transition to anaerobiosis, confirmed by the elevated lactate levels as observed in our previous associated study, and by the development of cellular stress, which was expressed by an accumulation of lipid peroxidation products. An earlier hypothesis, based on freshwater individuals of the same species, has been confirmed whereby the increased thermotolerance of G. lacustris from the saltwater lake was caused by differences in energy metabolism and energy supply of nonspecific cellular stress-response mechanisms. With the development of global climate change, these reactions could be advantageous for saltwater G. lacustris. The studied biochemical reactions can be used as biomarkers for the stress status of aquatic organisms when their habitat temperature changes.

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Scopus
Держатели документа:
Irkutsk State Univ, Inst Biol, Irkutsk, Russia.
Baikal Res Ctr, Irkutsk, Russia.
Belarusian State Univ, Int Sakharov Environm Inst, Minsk, BELARUS.
SB RAS, Inst Biophys, Krasnoyarsk Res Ctr, Krasnoyarsk, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.

Доп.точки доступа:
Vereshchagina, Kseniya; Kondrateva, Elizaveta; Axenov-Gribanov, Denis; Shatilina, Zhanna; Khomich, Andrey; Bedulina, Daria; Zadereev, Egor; Timofeyev, Maxim; Russian Science Foundation [17-14-01063]; Russian Foundation for Basic Research [16-34-00687, 16-34-60060, 17-34-50012]; Goszadanie project joint program of DAAD [6.9654.2017/8.9]; Ministry of education and Science M. Lomonosov [6.12735.2018/12.2]; Lake Baikal Foundation [FOB_02-3/05]

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The role of acidosis in the pathogenesis of severe forms of COVID-19 / Y. D. Nechipurenko, D. A. Semyonov, I. A. Lavrinenko [et al.] // Biology. - 2021. - Vol. 10, Is. 9. - Ст. 852, DOI 10.3390/biology10090852 . - ISSN 2079-7737
Кл.слова (ненормированные):
Acidosis -- Bohr effect -- COVID-19 -- Hypoxia -- Lactate -- PH -- SARS-CoV-2 -- Saturation
Аннотация: COVID-19 has specific characteristics that distinguish this disease from many other infec-tions. We suggest that the pathogenesis of severe forms of COVID-19 can be associated with acidosis. This review article discusses several mechanisms potentially linking the damaging effects of COVID-19 with acidosis and shows the existence of a vicious cycle between the development of hypoxia and acidosis in COVID-19 patients. At the early stages of the disease, inflammation, difficulty in gas exchange in the lungs and thrombosis collectively contribute to the onset of acidosis. In accordance with the Verigo-Bohr effect, a decrease in blood pH leads to a decrease in oxygen saturation, which contributes to the exacerbation of acidosis and results in a deterioration of the patient’s condition. A decrease in pH can also cause conformational changes in the S-protein of the virus and thus lead to a decrease in the affinity and avidity of protective antibodies. Hypoxia and acidosis lead to dysregu-lation of the immune system and multidirectional pro-and anti-inflammatory reactions, resulting in the development of a “cytokine storm”. In this review, we highlight the potential importance of supporting normal blood pH as an approach to COVID-19 therapy. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Scopus
Держатели документа:
Laboratory DNA-Protein Recognition, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russian Federation
Institute of Molecular Medicine and Pathobiochemistry, Voyno-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, 660022, Russian Federation
Institute of Biophysics Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Department of Human and Animal Physiology, Faculty of Medicine and Biology, Voronezh State University, Voronezh, 394018, Russian Federation
Department of Biological and Medical Physics, Moscow Institute of Physics and Technology, Moscow Region, Dolgoprudny, 141701, Russian Federation
Department of Biophysics, Faculty of Physics, Lomonosov Moscow State University, Moscow, 119991, Russian Federation
Laboratory of Medical Analytical Methods and Devices, Institute for Analytical Instrumentation of the Russian Academy of Sciences, St. Petersburg, 198095, Russian Federation
Sendai Viralytics LLC, Acton, MA 117261, United States
Laboratory of Cellular Bases for the Development of Malignant Diseases, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russian Federation

Доп.точки доступа:
Nechipurenko, Y. D.; Semyonov, D. A.; Lavrinenko, I. A.; Lagutkin, D. A.; Generalov, E. A.; Zaitceva, A. Y.; Matveeva, O. V.; Yegorov, Y. E.

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The Role of Acidosis in the Pathogenesis of Severe Forms of COVID-19 / Y. D. Nechipurenko, D. A. Semyonov, I. A. Lavrinenko [et al.] // Biology-Basel. - 2021. - Vol. 10, Is. 9. - Ст. 852, DOI 10.3390/biology10090852. - Cited References:86. - This research was funded by the Presidium of the Russian Academy of Sciences for Molecular and Cellular Biology and the Program of Fundamental Research for State Academies for years 2013-2020, project no. 01201363818. . - ISSN 2079-7737

РУБ Biology
Рубрики:
RESPIRATORY-ACIDOSIS
   LACTATE
   COAGULATION
   GLYCOLYSIS
   SECRETION
Кл.слова (ненормированные):
SARS-CoV-2 -- COVID-19 -- acidosis -- hypoxia -- saturation -- Bohr effect -- lactate -- pH
Аннотация: Simple Summary Recently, several studies have shown that acidosis, which is increased acidity in the blood and other body tissues, is often associated with severe COVID-19. In this article, we look at the mechanisms and consequences of acidosis that can lead to an exacerbation of COVID-19. We want to draw the attention of readers to the threshold values of such disease characteristics as hypoxia and acidosis, which are associated with a sharp deterioration in the patient's condition. Hypoxia and acidosis mutually reinforce each other according to the principle of a vicious cycle (that is, they are involved in a system of positive feedbacks). Elevated blood lactate (lactic acid) levels are associated with poor clinical outcomes in COVID patients. As a practical recommendation, we propose to pay more attention to the prevention of acidosis, including in the early stages of the disease, when the adjustment of homeostasis requires less effort and is less risky. COVID-19 has specific characteristics that distinguish this disease from many other infections. We suggest that the pathogenesis of severe forms of COVID-19 can be associated with acidosis. This review article discusses several mechanisms potentially linking the damaging effects of COVID-19 with acidosis and shows the existence of a vicious cycle between the development of hypoxia and acidosis in COVID-19 patients. At the early stages of the disease, inflammation, difficulty in gas exchange in the lungs and thrombosis collectively contribute to the onset of acidosis. In accordance with the Verigo-Bohr effect, a decrease in blood pH leads to a decrease in oxygen saturation, which contributes to the exacerbation of acidosis and results in a deterioration of the patient's condition. A decrease in pH can also cause conformational changes in the S-protein of the virus and thus lead to a decrease in the affinity and avidity of protective antibodies. Hypoxia and acidosis lead to dysregulation of the immune system and multidirectional pro- and anti-inflammatory reactions, resulting in the development of a "cytokine storm". In this review, we highlight the potential importance of supporting normal blood pH as an approach to COVID-19 therapy.

WOS
Держатели документа:
Russian Acad Sci, Engelhardt Inst Mol Biol, Lab DNA Prot Recognit, Moscow 119991, Russia.
Voyno Yasenetsky Krasnoyarsk State Med Univ, Inst Mol Med & Pathobiochem, Krasnoyarsk 660022, Russia.
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.
Voronezh State Univ, Fac Med & Biol, Dept Human & Anim Physiol, Voronezh 394018, Russia.
Moscow Inst Phys & Technol, Dept Biol & Med Phys, Dolgoprudnyi 141701, Russia.
Lomonosov Moscow State Univ, Fac Phys, Dept Biophys, Moscow 119991, Russia.
Russian Acad Sci, Lab Med Analyt Methods & Devices, Inst Analyt Instrumentat, St Petersburg 198095, Russia.
Sendai Viralyt LLC, Acton, MA USA.
Russian Acad Sci, Engelhardt Inst Mol Biol, Lab Cellular Bases Dev Malignant Dis, Moscow 119991, Russia.

Доп.точки доступа:
Nechipurenko, Yury D.; Semyonov, Denis A.; Lavrinenko, Igor A.; Lagutkin, Denis A.; Generalov, Evgenii A.; Zaitceva, Anna Y.; Matveeva, Olga, V; Yegorov, Yegor E.; Lagutkin, Denis; Presidium of the Russian Academy of Sciences for Molecular and Cellular Biology; Program of Fundamental Research for State Academies for years 2013-2020 [01201363818]

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