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Degradation of P(3HB) and P(3HB-co-3HV) in biological media / E. I. Shishatskaya [et al.] // Journal of Biomaterials Science, Polymer Edition. - 2005. - Vol. 16, Is. 5. - P643-657, DOI 10.1163/1568562053783678 . - ISSN 0920-5063
Кл.слова (ненормированные):
Degradation rate -- Fiber properties -- Morphology -- Poly(hydroxybutyrate-co-hydroxyvalerate) (P(3HB-co-3HV)) -- Polyhydroxyalkanoates(PHAs) -- Polyhydroxybutyrate (P(3HB)) -- Copolymers -- Degradation -- Differential scanning calorimetry -- Enzymes -- Morphology -- Scanning electron microscopy -- Tensile strength -- Tissue -- Transmission electron microscopy -- Degradation rate -- Fiber properties -- Polyhydroxyalkanoates (PHAs) -- Polyhydroxybutyrate (P(3HB)) -- Biopolymers -- buffer -- copolymer -- poly(3 hydroxybutyric acid) -- polyhydroxybutyrate hydroxyvalerate copolymer -- unclassified drug -- animal experiment -- animal model -- animal tissue -- article -- biodegradation -- controlled study -- crystal structure -- fiber -- giant cell -- in vitro study -- in vivo study -- macrophage -- morphology -- nonhuman -- pH -- priority journal -- rat -- structure analysis -- tensile strength -- tissue water -- weight reduction -- Animals -- Biodegradation, Environmental -- Buffers -- Humans -- Hydrogen-Ion Concentration -- Hydroxybutyrates -- Macrophages -- Microscopy, Electron, Scanning -- Microscopy, Electron, Transmission -- Muscle, Skeletal -- Polyesters -- Rats -- Rats, Wistar
Аннотация: The biodegradability of oriented fibers made of polyhydroxybutyrate (P(3HB)) and its co-polymer with ?-hydroxyvalerate (P(3HB-co-3HV)) was investigated in buffer solutions and in biological media in vitro and in vivo. The fibers of both polymer types demonstrated resistance to hydrolytic degradation in buffer solutions at 38В°C and pH from 4.5 to 7.0 (for up to 180 days). It has been found that the biodegradation of the fibers in vitro in blood and serum and in vivo is accompanied by weight losses and minor changes in the microstructure with no significant losses in the tensile strength over a long time (up to 180 days). The biodegradation rate of the less crystalline co-polymer P(3HB-co-3HV) fibers was 1.4-2.0-times higher than that of the homopolymer P(3HB). It has also been shown that the degradation of the fibers in vivo is influenced both by tissue fluid enzymes and cells (macrophages and foreign-body giant cells). The fibers were eroded on the surface only with no gross defects and no dramatic effects on their mechanical performance. В© VSP 2005.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 600326, Russian Federation
Department of Cardiac Surgery, University of Glasgow, Royal Infirmary, 10 Alexandra Parade, Glasgow G3, United Kingdom : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Gordeev, S.A.; Puzyr, A.P.

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Microencapculation of daunorubicin with biodegradable polymer matrix / E. I. Shishatskaya // Antibiotiki i Khimioterapiya. - 2007. - Vol. 52, Is. 9-10. - С. 3-8 . - ISSN 0235-2990
Кл.слова (ненормированные):
Daunorubicin -- Microencapsulation -- Polymer matrix -- antineoplastic antibiotic -- daunorubicin -- polyester -- animal -- article -- Bagg albino mouse -- chemistry -- kinetics -- microcapsule -- mouse -- particle size -- Animals -- Antibiotics, Antineoplastic -- Capsules -- Daunorubicin -- Kinetics -- Mice -- Mice, Inbred BALB C -- Particle Size -- Polyesters -- Animalia
Аннотация: Procedure for microencapsulation providing stable formation of high quality microspheres was designed. Conditions for deposition of the anthracycline antibiotic daunorubicin to polymer matrix were developed and microsheres loaded with various quantities of the drug were prepared. The kinetics of the in vitro and in vivo release of daunorubicin from the microsheres was studied. The rate of the rubomycin release to the medium in vitro (balanced phosphate buffer at 37.8В°C) in a 300-hour experiment directly depended on the quantity of the incorporated drug and averaged 0.81 В· 10 -4 to 2.3 В· 10 -4 mcg/mlВ·h. The experiment on laboratory animals with intraperitoneal administration of the rubomycin microsperes showed that the drug remained in the blood and abdominal liquid for a long time (up to 10 days). Possible control of the quantity of the rubomycin encapsulation to the polymer matrix, no sharp efflux of the drug at the early stages of the observation and low rate of the drug release to the medium allowed to conclude that the use of the biodegradable polymer microsperes as carriers of the high toxic antibiotic providing its prolonged action was prospective.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.

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Characterization of polymeric microparticles based on resorbable polyesters of oxyalkanoic acids as a platform for deposition and delivery of drugs / A. V. Goreva [et al.] // Polym. Sci. Ser. A. - 2012. - Vol. 54, Is. 2. - P94-105, DOI 10.1134/S0965545X12020022. - Cited References: 33. - This work was supported by the program for Support of Leading Scientific Schools of the Russian Federation (project no. 11.G34.31.0013.2010, Biotechnology of New Biomaterials) and the program of integrated studies of the Presidium of the Siberian Branch, Russian Academy of Sciences (project no. 93). . - 12. - ISSN 0965-545X

РУБ Polymer Science
Рубрики:
IN-VITRO RELEASE
   POLYHYDROXYBUTYRATE MICROSPHERES
   BLENDS
   RIFAMPICIN
   BIOCOMPATIBILITY
   DEGRADATION
   FORMULATION
   COMPOSITE
   CARRIERS
   MODEL
Аннотация: The effect of the preparation technique (chemical composition of a polymer, type and method of emulsion mixing, and molecular mass of a drug) on the yield, structure, and size of microparticles obtained from resorbable polyesters of microbiological origin, polyhydroxyalkanoates, is studied. It is found that the concentration of the polymer solution and the method of emulsion mixing are the most significant factors affecting the diameter of microparticles based on polyhydroxyalkanoates; the surface structure of particles depends to a higher extent on the chemical composition of the polymer. The family of microparticles from 100-200 nm to 50-70 mu m in diameter is synthesized. It is shown that the rate of drug release from microparticles in vitro into the medium is higher in the case of 3-hydroxybutyrate copolymers with 3-hydroxyvalerate than in the case of the homopolymer of 3-hydroxybutyrate. This parameter increases with the content of 3-hydroxyvalerate units in the copolymer and the porosity and mass fraction of the drug in particles with a decrease in their sizes. For in vitro systems containing a phosphate buffer, variation in the preparation parameters makes it possible to obtain microparticles with various characteristics suitable for deposition of drugs. For microparticles obtained from polyhydroxyalkanoates and having different diameters, the mathematical description of the kinetics of drug release from the polymer matrix is provided.

Держатели документа:
[Goreva, A. V.
Shishatskaya, E. I.
Volova, T. G.] Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
[Shishatskaya, E. I.
Volova, T. G.] Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Krasnoyarsk 660041, Russia
[Goreva, A. V.
Sinskey, A. J.] MIT, Cambridge, MA 02139 USA : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Goreva, A.V.; Shishatskaya, E.I.; Volova, T.G.; Sinskey, A.J.

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Ultrafast fluorescence relaxation spectroscopy of 6,7-dimethyl-(8-ribityl)-lumazine and riboflavin, free and bound to antenna proteins from bioluminescent bacteria / V. N. Petushkov [et al.] // Journal of Physical Chemistry B. - 2003. - Vol. 107, Is. 39. - P10934-10939 . - ISSN 1520-6106
Кл.слова (ненормированные):
Bacteria -- Bioluminescence -- Chemical relaxation -- Chromophores -- Dielectric properties -- Proteins -- Solvents -- Bioluminescent bacteria -- Dimethyl ribityl lumazine -- Photobacterium leiognathi -- Riboflavin -- Ultrafast fluorescence relaxation spectroscopy -- Fluorescence
Аннотация: The solvation dynamics of interesting bioluminescent chromophores have been determined, using subpicosecond and wavelength-resolved fluorescence spectroscopy, in combination with global analysis of the multidimensional data sets. The systems investigated comprise the free ligands 6,7-dimethyl-(8-ribityl)-lumazine (lumazine) and riboflavin in an aqueous buffer and both ligands when noncovalently bound to two bacterial bioluminescent antenna proteins: lumazine protein (from Photobacterium leiognathi) and the blue fluorescent protein (from Vibrio fischeri Y1). Fluorescence spectral relaxation of the free ligands is complete within a few picoseconds. Subsequently, the fluorescence intensity increases by ?7% on a time scale of 15-30 ps. Fluorescence spectral relaxation of the protein-bound ligands is largely complete within 1 ps but reveals a small red shift with a minor, but distinctly longer, relaxation time than that of the free ligands, which is tentatively assigned to the relaxation of protein-bound water in the vicinity of the excited chromophore.

Scopus
Держатели документа:
MicroSpectroscopy Centre, Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, Netherlands
Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit, De Boelelaan 1081, 1081 HV Amsterdam, Netherlands
Dept. of Biochem. and Molec. Biology, University of Georgia, Athens, GA 30602, United States
Department of Structural Biology, Faculty of Earth and Life Sciences, Vrije Universiteit, De Boelelaan 1087, 1081 HV Amsterdam, Netherlands
Institute of Biophysics, Academy of Sciences of Russia, Krasnoyarsk 660036, Russian Federation
IPMC, Universite de Lausanne, CH 1015 Lausanne, Switzerland : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V.N.; Van Stokkum, I.H.M.; Gobets, B.; Van Mourik, F.; Lee, J.; Van Grondelle, R.; Visser, A.J.W.G.

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INACTIVATION OF BACTERIAL LUCIFERASES BY N-ETHYLMALEIMIDE [Text] / T. P. SANDALOVA, N. A. TYULKOVA // Biochem.-Moscow. - 1992. - Vol. 57, Is. 6. - P. 552-558. - Cited References: 21 . - ISSN 0006-2979

РУБ Biochemistry & Molecular Biology
Рубрики:
AMINO-ACID SEQUENCE
   NUCLEOTIDE-SEQUENCE
   REACTIVE SULFHYDRYL
   PHOTOBACTERIUM-LEIOGNATHI
   VIBRIO-HARVEYI
   BIOLUMINESCENCE
   SUBUNIT
   REGION
   GENE
Кл.слова (ненормированные):
LUCIFERASE -- N-ETHYLMALEIMIDE
Аннотация: The kinetics of inactivation of luciferases from four species of luminescent bacteria by the thiol reagent N-ethylmaleimide were investigated The dependencies of inactivation on ionic strength differed among the enzymes. Increasing the molarity of the buffer increased the rate of inactivation of all luciferases except that of Vibrio harveyi. Modification of Photobacterium phosphoreum luciferase decreased the maximal intensity of bioluminescence, whereas modification of Photobacterium leiognathi and Vibrio fischeri luciferases in high ionic strength buffers decreased the maximal intensity of bioluminescence and changed the luminescence decay rate constant. High ionic strength apparently alters the conformational states of the luciferases.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
SANDALOVA, T.P.; TYULKOVA, N.A.

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Ultrafast fluorescence relaxation spectroscopy of 6,7-dimethyl-(8-ribityl)-lumazine and riboflavin, free and bound to antenna proteins from bioluminescent bacteria [Text] / V. N. Petushkov [et al.] // J. Phys. Chem. B. - 2003. - Vol. 107, Is. 39. - P. 10934-10939, DOI 10.1021/jp034266e. - Cited References: 52 . - ISSN 1520-6106

РУБ Chemistry, Physical
Рубрики:
TIME-RESOLVED FLUORESCENCE
   VIBRIO-FISCHERI Y1
   FEMTOSECOND SOLVATION DYNAMICS
   FLAVIN ADENINE-DINUCLEOTIDE
   PHOTOBACTERIUM-LEIOGNATHI
   BIOLOGICAL WATER
   SOLVENT DYNAMICS
   DIELECTRIC-RELAXATION
   MOLECULAR-DYNAMICS
   TRYPTOPHAN
Аннотация: The solvation dynamics of interesting bioluminescent chromophores have been determined, using subpicosecond and wavelength-resolved fluorescence spectroscopy, in combination with global analysis of the multidimensional data sets. The systems investigated comprise the free ligands 6,7-dimethyl-(8-ribityl)-lumazine (lumazine) and riboflavin in an aqueous buffer and both ligands when noncovalently bound to two bacterial bioluminescent antenna proteins: lumazine protein (from Photobacterium leiognathi) and the blue fluorescent protein (from Vibrio fischeri Y1). Fluorescence spectral relaxation of the free ligands is complete within a few picoseconds. Subsequently, the fluorescence intensity increases by similar to7% on a time scale of 15-30 ps. Fluorescence spectral relaxation of the protein-bound ligands is largely complete within 1 ps but reveals a small red shift with a minor, but distinctly longer, relaxation time than that of the free ligands, which is tentatively assigned to the relaxation of protein-bound water in the vicinity of the excited chromophore.

WOS
Держатели документа:
Univ Wageningen & Res Ctr, Biochem & Biophys Lab, MicroSpect Ctr, NL-6703 HA Wageningen, Netherlands
Vrije Univ Amsterdam, Fac Sci & Engn, Dept Phys & Astron, NL-1081 HV Amsterdam, Netherlands
Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA
Vrije Univ Amsterdam, Fac Earth & Life Sci, Dept Biol Struct, NL-1081 HV Amsterdam, Netherlands
Russian Acad Sci, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V.N.; van Stokkum, IHM; Gobets, B...; van Mourik, F...; Lee, J...; van Grondelle, R...; Visser, AJWG

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Bacteria of Lake Pyasino and Adjacent Rivers after an Accidental Diesel Spill in 2020 / O. V. Kolmakova, M. Y. Trusova, O. A. Baturina, M. R. Kabilov // Contemp. Probl. Ecol. - 2021. - Vol. 14, Is. 4. - P356-367, DOI 10.1134/S1995425521040053. - Cited References:22. - This work was supported by economic agreement no. 223-EP-2020/07 with the Siberian Branch of the Russian Academy of Sciences and the Project Office for the Development of the Arctic (PORA), and State Task of the Fundamental Research Program of the Russian Federation, topic no. 51.1.1. Bioinformatics analysis was supported by basic budget funding from the ICBFM SB RAS (AAAAA17-117020210021-7). . - ISSN 1995-4255. - ISSN 1995-4263

РУБ Ecology
Рубрики:
SP-NOV.
   GEN. NOV.
   OIL
   BACTERIOPLANKTON
   SEQUENCES
Кл.слова (ненормированные):
bacterioplankton -- sediments -- high-throughput sequencing -- oil spill -- Lake -- Pyasino -- 16S rRNA
Аннотация: The bacterial composition of water and sediments in Lake Pyasino and adjacent rivers has been studied by high-throughput sequencing for the first time. No hydrocarbon-degrading bacteria are found in the river sections exposed to the diesel spillage in May 2020. The Daldykan and Ambarnaya rivers, located downstream of the spill site, are dominated by bacteria that oxidize sulfur and heavy metals. At the same time, these bacteria are not found in Lake Pyasino, which is dominated by small cyanobacteria. Thus, the composition of bacteria in the water and sediments of the Norilsk-Pyasino lake-river system corresponds to the technogenic impact to which it is exposed and also demonstrates the buffer properties of Lake Pyasino as water flows into the Kara Sea.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci Ctr, Inst Biophys, Krasnoyarsk 660036, Akademgorodok, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Siberian Branch, Inst Chem Biol & Fundamental Med, Genom Core Facil, Novosibirsk 630090, Russia.

Доп.точки доступа:
Kolmakova, O. V.; Trusova, M. Yu.; Baturina, O. A.; Kabilov, M. R.; Siberian Branch of the Russian Academy of SciencesRussian Academy of Sciences [223-EP-2020/07]; Project Office for the Development of the Arctic (PORA); State Task of the Fundamental Research Program of the Russian Federation [51.1.1]; ICBFM SB RAS [AAAAA17-117020210021-7]

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